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[PMID]:28457531
[Au] Autor:Kaufman G; Skrtic D
[Ad] Endereço:Volpe Research Center, ADA Foundation, Gaithersburg, MD 20899, USA. Electronic address: gili.kaufman@nist.gov.
[Ti] Título:Spatial development of gingival fibroblasts and dental pulp cells: Effect of extracellular matrix.
[So] Source:Tissue Cell;49(3):401-409, 2017 Jun.
[Is] ISSN:1532-3072
[Cp] País de publicação:Scotland
[La] Idioma:eng
[Ab] Resumo:Cells sensing changes in their microenvironmental stiffness and composition alter their responses, accordingly. This study determines whether gingival fibroblasts (GFs) and dental pulp mesenchymal stem cells (DPMSCs) support the formation of continuous layers in vitro by mimicking the stiffness and protein composition of their native extracellular matrix (ECM). Immortalized cells were incubated with (i) 0-100% Matrigel-ECM (M-ECM) for 7-28d, and with (ii) collagen and fibrin matrices for 14d. Cultures were analyzed by phase-contrast, fluorescence and confocal microscopies. The diameters and surface areas were measured via ImageJ. Self-renewal markers were detected by RT-PCR and immunocytochemistry assays. GFs and DPMSCs developed spheroids interconnected by elongated cell bundles or layers, respectively, expressing the self-renewal markers. Increased matrix stiffness resulted in spheroids replacement by the interconnecting cells/layers. Both cells required 100% M-ECM to reduce their spheroid diameter. However, it reduced the surface area of the interconnecting layers. Those differences led to extended, spindle-shaped GFs vs. compact, ring-shaped DPMSCs constructs. Collagen and fibrin matrices developed continuous layers of tightly connected cells vs. distinctive scattered cell aggregates, respectively. The ability of GFs and DPMSCs to create tissue-like multicellular layers at various matrix conditions may be imprinted by cells' adaptation to mechanical forces and composition in vivo.
[Mh] Termos MeSH primário: Polpa Dentária/metabolismo
Matriz Extracelular/química
Fibroblastos/metabolismo
Gengiva/metabolismo
[Mh] Termos MeSH secundário: Animais
Linhagem Celular Transformada
Polpa Dentária/citologia
Matriz Extracelular/metabolismo
Fibroblastos/citologia
Gengiva/citologia
Camundongos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE


  2 / 15259 MEDLINE  
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[PMID]:29458668
[Au] Autor:Al-Ahmad A; Muzafferiy F; Anderson AC; Wölber JP; Ratka-Krüger P; Fretwurst T; Nelson K; Vach K; Hellwig E
[Ad] Endereço:1​Department of Operative Dentistry and Periodontology, Faculty of Medicine, Medical Center - University of Freiburg, Germany.
[Ti] Título:Shift of microbial composition of peri-implantitis-associated oral biofilm as revealed by 16S rRNA gene cloning.
[So] Source:J Med Microbiol;67(3):332-340, 2018 Mar.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PURPOSE: Micro-organisms are important triggers of peri-implant inflammation and analysing their diversity is necessary for peri-implantitis treatment. This study aimed to analyse and compare the microbiota associated with individuals with peri-implantitis, as well as clinically healthy implant sites. METHODOLOGY: Subgingival biofilm samples were taken from 10 individuals with peri-implantitis and from at least 1 clinically healthy implant. DNA was extracted and bacterial 16S rRNA genes were amplified using universal primers. After cloning the PCR-products, amplified inserts of positive clones were digested using restriction endonucleases, and the chosen clones were sequenced. The 16S rDNA-sequences were compared to those from the public sequence databases GenBank, EMBL and DDBJ to determine the corresponding taxa. RESULTS: Differing distributions of taxa belonging to the phyla Firmicutes, Bacteroidetes, Fusobacteria, Actinobacteria, Proteobacteria, Synergistetes, Spirochaetae and TM 7 were detected in both the healthy implant (HI) and the peri-implantitis (PI) groups. A significantly higher relative abundance of phylum Bacteroidetes, as well as of the species Fusobacterium nucleatum, were found in the PI group (P<0.05). The putative periodontal red complex (Porphyromonas gingivalis, Tannerella forsythia) was also detected at significantly higher levels in the PI group (P<0.05), whereas the yellow group, as well as the species Veillonella dispar, tended to be associated with the HI group. CONCLUSION: A shift in the healthy subgingival microbiota was shown in peri-implantitis-associated biofilm. Anaerobic Gram-negative periopathogens, including P. gingivalis and T. forsythia, seem to play an important role in peri-implantitis development and should be considered in treatment and prevention strategies.
[Mh] Termos MeSH primário: Bactérias/isolamento & purificação
Biofilmes
Microbiota/genética
Peri-Implantite/microbiologia
RNA Ribossômico 16S/genética
[Mh] Termos MeSH secundário: Idoso
Idoso de 80 Anos ou mais
Bactérias/classificação
Bactérias/genética
Carga Bacteriana
Fenômenos Fisiológicos Bacterianos
Bacteroides/genética
Bacteroides/isolamento & purificação
Feminino
Fusobacterium nucleatum/genética
Fusobacterium nucleatum/isolamento & purificação
Genes de RNAr
Gengiva/microbiologia
Seres Humanos
Masculino
Meia-Idade
Porphyromonas gingivalis/genética
Porphyromonas gingivalis/isolamento & purificação
Prevotella intermedia/genética
Prevotella intermedia/isolamento & purificação
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180221
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000682


  3 / 15259 MEDLINE  
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[PMID]:29366789
[Au] Autor:Kim MS; Shin DM; Kim MS
[Ad] Endereço:Center for Metabolic Function Regulation, Wonkwang University, School of Medicine, No. 460 Iksan-Daero, Iksan, Jeonbuk 54538, Republic of Korea.
[Ti] Título:Acidification induces OGR1/Ca /calpain signaling in gingival fibroblasts.
[So] Source:Biochem Biophys Res Commun;496(2):693-699, 2018 02 05.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Gingivitis, the mildest form of periodontitis, is generally considered a consequence of prolonged exposure of the gingiva to periodontal pathogens. On the other hand, several epidemiologic reports have suggested that other etiologic factors such as oral acidification may also increase the susceptibility of the periodontium to destruction. However, the pathologic mechanism underlying the effects of oral acidification on the gingiva is still largely unknown. In this study, we analyzed molecular pathways mediating the influence of the acidic environment on human gingival fibroblasts (HGFs). Acidic extracellular pH caused biphasic increase of intracellular Ca level ([Ca ] ) through activation of ovarian cancer G protein-coupled receptor 1, phospholipase C, and Ca release from the endoplasmic reticulum, but not through voltage-gated Ca channels or extracellular Ca influx via transient receptor potential cation channel subfamily V member 1. The acidic environment was also transiently cytotoxic for HGFs; however, the activation of pro-apoptotic proteins poly (ADP-ribose) polymerase-1 and BAX was not observed. Furthermore, we found that intracellular matrix metalloproteinase 1 was consistently upregulated in HGFs grown in regular medium, but significantly reduced in the acidic medium, which depended on [Ca ] increase, lysosomal pH homeostasis, and Ca -dependent protease calpain. Considering that HGFs, essential for oral wound healing, in the in vitro culture system are placed in wound repair-like conditions, our findings provide important insights into molecular mechanisms underlying HGF functional impairment and chronic damage to the gingiva caused by the acidic intraoral environment.
[Mh] Termos MeSH primário: Cálcio/metabolismo
Calpaína/metabolismo
Fibroblastos/citologia
Gengiva/citologia
Receptores Acoplados a Proteínas-G/metabolismo
Transdução de Sinais
[Mh] Termos MeSH secundário: Linhagem Celular
Fibroblastos/metabolismo
Gengiva/metabolismo
Seres Humanos
Concentração de Íons de Hidrogênio
Lisossomos/metabolismo
Metaloproteinase 1 da Matriz/metabolismo
Fosfolipases Tipo C/metabolismo
Cicatrização
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (GPR68 protein, human); 0 (Receptors, G-Protein-Coupled); EC 3.1.4.- (Type C Phospholipases); EC 3.4.22.- (Calpain); EC 3.4.24.7 (Matrix Metalloproteinase 1); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180126
[St] Status:MEDLINE


  4 / 15259 MEDLINE  
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[PMID]:29190775
[Au] Autor:Ahn SH; Chun S; Park C; Lee JH; Lee SW; Lee TH
[Ad] Endereço:Department of Oral Biochemistry, Dental Science Research Institute, Medical Research Center for Biomineralization Disorders, School of Dentistry, Chonnam National University, Gwangju, Republic of Korea.
[Ti] Título:Transcriptome profiling analysis of senescent gingival fibroblasts in response to Fusobacterium nucleatum infection.
[So] Source:PLoS One;12(11):e0188755, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Periodontal disease is caused by dental plaque biofilms. Fusobacterium nucleatum is an important periodontal pathogen involved in the development of bacterial complexity in dental plaque biofilms. Human gingival fibroblasts (GFs) act as the first line of defense against oral microorganisms and locally orchestrate immune responses by triggering the production of reactive oxygen species and pro-inflammatory cytokines (IL-6 and IL-8). The frequency and severity of periodontal diseases is known to increase in elderly subjects. However, despite several studies exploring the effects of aging in periodontal disease, the underlying mechanisms through which aging affects the interaction between F. nucleatum and human GFs remain unclear. To identify genes affected by infection, aging, or both, we performed an RNA-Seq analysis using GFs isolated from a single healthy donor that were passaged for a short period of time (P4) 'young GFs' or for longer period of time (P22) 'old GFs', and infected or not with F. nucleatum. Comparing F. nucleatum-infected and uninfected GF(P4) cells the differentially expressed genes (DEGs) were involved in host defense mechanisms (i.e., immune responses and defense responses), whereas comparing F. nucleatum-infected and uninfected GF(P22) cells the DEGs were involved in cell maintenance (i.e., TGF-ß signaling, skeletal development). Most DEGs in F. nucleatum-infected GF(P22) cells were downregulated (85%) and were significantly associated with host defense responses such as inflammatory responses, when compared to the DEGs in F. nucleatum-infected GF(P4) cells. Five genes (GADD45b, KLF10, CSRNP1, ID1, and TM4SF1) were upregulated in response to F. nucleatum infection; however, this effect was only seen in GF(P22) cells. The genes identified here appear to interact with each other in a network associated with free radical scavenging, cell cycle, and cancer; therefore, they could be potential candidates involved in the aged GF's response to F. nucleatum infection. Further studies are needed to confirm these observations.
[Mh] Termos MeSH primário: Fusobacterium nucleatum/patogenicidade
Perfilação da Expressão Gênica
Gengiva/metabolismo
Transcriptoma
[Mh] Termos MeSH secundário: Células Cultivadas
Senescência Celular
Fibroblastos/metabolismo
Fibroblastos/microbiologia
Gengiva/citologia
Gengiva/microbiologia
Seres Humanos
Análise de Sequência de RNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180213
[Lr] Data última revisão:
180213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171201
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0188755


  5 / 15259 MEDLINE  
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[PMID]:28468157
[Au] Autor:Vieira BS; de Oliveira AR; Rodas MR; Maia LP; Dos Santos PL; Silveira EMV
[Ad] Endereço:*Pinelli Henriques Center West School (COPH) †Sagrado Coração Univeristy (USC), Bauru ‡University of Western São Paulo (UNOESTE), Presidente Prudente, São Paulo, Brazil.
[Ti] Título:Comparison of Two Screw-Retained Free Gingival Grafting Techniques.
[So] Source:J Craniofac Surg;28(3):746-749, 2017 May.
[Is] ISSN:1536-3732
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Free gingival graft is a predictable technique for increasing the amount of attached gingiva and root coverage; however, its use is limited for cosmetic reasons. To overcome this issue, this study sought to compare 2 free gingival graft techniques that use oral screws to attach grafts. Free gingival graft was performed on teeth 44 to 46 using the traditional technique, while on the opposite side, on teeth 34 to 36, partly epithelialized free gingival grafts were performed. The partly epithelialized free gingival grafts were found to provide better cosmetic results relative to the completely epithelialized free gingival graft, and the use of stabilizing screws was found to be simple and effective.
[Mh] Termos MeSH primário: Parafusos Ósseos
Retalhos de Tecido Biológico
Gengiva/transplante
Retração Gengival/cirurgia
Procedimentos Cirúrgicos Bucais/métodos
Raiz Dentária/cirurgia
[Mh] Termos MeSH secundário: Adulto
Feminino
Seres Humanos
[Pt] Tipo de publicação:CASE REPORTS; COMPARATIVE STUDY; JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180209
[Lr] Data última revisão:
180209
[Sb] Subgrupo de revista:D
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1097/SCS.0000000000003460


  6 / 15259 MEDLINE  
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[PMID]:29267310
[Au] Autor:Kido D; Mizutani K; Takeda K; Mikami R; Matsuura T; Iwasaki K; Izumi Y
[Ad] Endereço:Department of Periodontology, Graduate school of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), Tokyo, Japan.
[Ti] Título:Impact of diabetes on gingival wound healing via oxidative stress.
[So] Source:PLoS One;12(12):e0189601, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The aim of this study is to investigate the mechanisms linking high glucose to gingival wound healing. Bilateral wounds were created in the palatal gingiva adjacent to maxillary molars of control rats and rats with streptozotocin-induced diabetes. After evaluating postsurgical wound closure by digital imaging, the maxillae including wounds were resected for histological examinations. mRNA expressions of angiogenesis, inflammation, and oxidative stress markers in the surgical sites were quantified by real-time polymerase chain reaction. Primary fibroblast culture from the gingiva of both rats was performed in high glucose and normal medium. In vitro wound healing and cell proliferation assays were performed. Oxidative stress marker mRNA expressions and reactive oxygen species production were measured. Insulin resistance was evaluated via PI3K/Akt and MAPK/Erk signaling following insulin stimulation using Western blotting. To clarify oxidative stress involvement in high glucose culture and cells of diabetic rats, cells underwent N-acetyl-L-cysteine treatment; subsequent Akt activity was measured. Wound healing in diabetic rats was significantly delayed compared with that in control rats. Nox1, Nox2, Nox4, p-47, and tumor necrosis factor-α mRNA levels were significantly higher at baseline in diabetic rats than in control rats. In vitro study showed that cell proliferation and migration significantly decreased in diabetic and high glucose culture groups compared with control groups. Nox1, Nox2, Nox4, and p47 expressions and reactive oxygen species production were significantly higher in diabetic and high glucose culture groups than in control groups. Akt phosphorylation decreased in the high glucose groups compared with the control groups. Erk1/2 phosphorylation increased in the high glucose groups, with or without insulin treatment, compared with the control groups. Impaired Akt phosphorylation partially normalized after antioxidant N-acetyl-L-cysteine treatment. Thus, delayed gingival wound healing in diabetic rats occurred because of impaired fibroblast proliferation and migration. Fibroblast dysfunction may occur owing to high glucose-induced insulin resistance via oxidative stress.
[Mh] Termos MeSH primário: Diabetes Mellitus Experimental/patologia
Gengiva/patologia
Estresse Oxidativo
Cicatrização
[Mh] Termos MeSH secundário: Acetilcisteína/farmacologia
Animais
Antioxidantes/farmacologia
Biomarcadores/metabolismo
Movimento Celular
Proliferação Celular
Células Cultivadas
Diabetes Mellitus Experimental/genética
Diabetes Mellitus Experimental/metabolismo
Expressão Gênica
Gengiva/efeitos dos fármacos
Insulina/metabolismo
Masculino
Ratos
Ratos Wistar
Espécies Reativas de Oxigênio/metabolismo
Cicatrização/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antioxidants); 0 (Biomarkers); 0 (Insulin); 0 (Reactive Oxygen Species); WYQ7N0BPYC (Acetylcysteine)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171222
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189601


  7 / 15259 MEDLINE  
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[PMID]:29235774
[Au] Autor:Javaheri DS
[Ti] Título:Achieving Natural Tissue Contours: A Predictable and Simplified Technique.
[So] Source:Dent Today;36(1):96, 98-9, 2017 Jan.
[Is] ISSN:8750-2186
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Implantação Dentária Endo-Óssea/métodos
Implantes Dentários para Um Único Dente
Estética Dentária
Gengiva/anatomia & histologia
[Mh] Termos MeSH secundário: Projeto do Implante Dentário-Pivô
Técnica de Moldagem Odontológica
Seres Humanos
Incisivo
Masculino
Maxila
Meia-Idade
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180115
[Lr] Data última revisão:
180115
[Sb] Subgrupo de revista:D
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE


  8 / 15259 MEDLINE  
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[PMID]:29235315
[Au] Autor:McArdle BF
[Ti] Título:Design Enhances Soft-Tissue Architecture.
[So] Source:Dent Today;36(4):84, 86, 88-9, 2017 Apr.
[Is] ISSN:8750-2186
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Implantação Dentária Endo-Óssea/métodos
Implantes Dentários
Planejamento de Prótese Dentária
Gengiva/cirurgia
[Mh] Termos MeSH secundário: Estética Dentária
Seres Humanos
Planejamento de Assistência ao Paciente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Dental Implants)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180115
[Lr] Data última revisão:
180115
[Sb] Subgrupo de revista:D
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE


  9 / 15259 MEDLINE  
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[PMID]:29231686
[Au] Autor:Soolari A; Kesten G; Soolari A
[Ti] Título:A Challenging Mandibular Anterior Implant Case.
[So] Source:Dent Today;36(5):90,92-3, 2017 May.
[Is] ISSN:8750-2186
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Periodontite Crônica/cirurgia
Implantação Dentária Endo-Óssea/métodos
Prótese Dentária Fixada por Implante
[Mh] Termos MeSH secundário: Idoso
Transplante Ósseo
Periodontite Crônica/diagnóstico por imagem
Tomografia Computadorizada de Feixe Cônico
Coroas
Implantes Dentários
Gengiva/transplante
Seres Humanos
Masculino
Mandíbula/diagnóstico por imagem
Mandíbula/cirurgia
Extração Dentária
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Dental Implants)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180115
[Lr] Data última revisão:
180115
[Sb] Subgrupo de revista:D
[Da] Data de entrada para processamento:171213
[St] Status:MEDLINE


  10 / 15259 MEDLINE  
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[PMID]:29231673
[Au] Autor:Winter RB
[Ti] Título:Practical Laser Applications in General Practice.
[So] Source:Dent Today;36(6):78, 80, 82-3, 2017 Jun.
[Is] ISSN:8750-2186
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Implantação Dentária Endo-Óssea
Odontologia Geral
Gengiva/cirurgia
Terapia a Laser
Lasers de Gás/uso terapêutico
Procedimentos Cirúrgicos Bucais/instrumentação
Alvéolo Dental/cirurgia
[Mh] Termos MeSH secundário: Dióxido de Carbono
Prótese Dentária Fixada por Implante
Feminino
Seres Humanos
Meia-Idade
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
142M471B3J (Carbon Dioxide)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180115
[Lr] Data última revisão:
180115
[Sb] Subgrupo de revista:D
[Da] Data de entrada para processamento:171213
[St] Status:MEDLINE



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