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Pesquisa : A14.549.167.900.720.255 [Categoria DeCS]
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[PMID]:28675007
[Au] Autor:Xi L; Guoqing C; Weidong T
[Ad] Endereço:State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Dept. of Oral and Maxillofacial Trauma and Plastic Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China;National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
[Ti] Título:[Effect of hypoxia on the biological characteristics of human dental follicle cells].
[So] Source:Hua Xi Kou Qiang Yi Xue Za Zhi;35(3):245-252, 2017 Jun 01.
[Is] ISSN:1000-1182
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:OBJECTIVE: This study aimed to investigate the effects of hypoxia on the characteristics of human dental follicle cells (hDFCs). METHODS: The tissue explant collagenase method was used to isolate hDFCs from young permanent teeth. The immunofluorescence technique was used to detect cell surface markers, and the multi-differentiation potential was detected by multilineage differentiation induction assay. Then, the hypoxic microenvironment was physically mimicked, and the cells were divided into the normoxia group (20%O2) and the hypoxia group (2%O2). The effects of hypoxia on cell migration and proliferation were examined by Transwell chamber test and CCK-8 assay, respectively. The gene and protein expression levels of stemness-related markers at both oxygen concentrations were measured by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot, respectively. After osteogenic induction of both groups, qRT-PCR was performed to evaluate the osteogenesis-related gene, and alizarin red staining was used to assess the formation of mineralized nodules. RESULTS: With the multi-differentiation capacity of osteogenic cells, adipogenic cells, and nerves, hDFCs demonstrate strong stem cell characteristics and possess the criteria of mesenchymal stem cells, which can meet the requirements of seed cells in dental tissue engineering. Hypoxia was conducive to the maintenance of hDFC stemness. Hypoxia promoted the migration and proliferation of hDFCs. The hDFCs were induced to osteogenic differentiation under hypoxic conditions, thereby enhancing osteogenesis. CONCLUSIONS: Hypoxic microenvironment plays an important role in maintaining the stemness and promoting the proliferation, migration, and differentiation of hDFCs. Thus, this microenvironment could also serve several important functions in future clinical applications.
[Mh] Termos MeSH primário: Hipóxia Celular
Saco Dentário
Osteogênese
[Mh] Termos MeSH secundário: Diferenciação Celular
Movimento Celular
Seres Humanos
Células Mesenquimais Estromais
Reação em Cadeia da Polimerase em Tempo Real
Células-Tronco
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170807
[Lr] Data última revisão:
170807
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:170705
[St] Status:MEDLINE
[do] DOI:10.7518/hxkq.2017.03.004


  2 / 569 MEDLINE  
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[PMID]:28541773
[Au] Autor:Xu QL; Furuhashi A; Zhang QZ; Jiang CM; Chang TH; Le AD
[Ad] Endereço:1 Department of Oral & Maxillofacial Surgery & Pharmacology, University of Pennsylvania School of Dental Medicine, Philadelphia, PA, USA.
[Ti] Título:Induction of Salivary Gland-Like Cells from Dental Follicle Epithelial Cells.
[So] Source:J Dent Res;96(9):1035-1043, 2017 Aug.
[Is] ISSN:1544-0591
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The dental follicle (DF), most often associated with unerupted teeth, is a condensation of ectomesenchymal cells that surrounds the tooth germ in early stages of tooth development. In the present study, we aim to isolate epithelial stem-like cells from the human DF and explore their potential differentiation into salivary gland (SG) cells. We demonstrated the expression of stem cell-related genes in the epithelial components of human DF tissues, and these epithelial progenitor cells could be isolated and ex vivo expanded in a reproducible manner. The human DF-derived epithelial cells possessed clonogenic and sphere-forming capabilities, as well as expressed a panel of epithelial stem cell-related genes, thus conferring stem cell properties (hDF-EpiSCs). When cultured under in vitro 3-dimensional induction conditions, hDF-EpiSCs were capable to differentiate into SG acinar and duct cells. Furthermore, transplantation of hDF-EpiSC-loaded native de-cellularized rat parotid gland scaffolds into the renal capsule of nude mice led to the differentiation of transplanted hDF-EpiSCs into salivary gland-like cells. These findings suggest that hDF-EpiSCs might be a promising source of epithelial stem cells for the development of stem cell-based therapy or bioengineering SG tissues to repair/regenerate SG dysfunction.
[Mh] Termos MeSH primário: Saco Dentário/citologia
Células Epiteliais/citologia
Glândulas Salivares/citologia
Engenharia Tecidual/métodos
[Mh] Termos MeSH secundário: Animais
Western Blotting
Técnicas de Cultura de Células
Diferenciação Celular
Células Cultivadas
Feminino
Seres Humanos
Imuno-Histoquímica
Camundongos
Camundongos Nus
Microscopia Confocal
Ratos
Ratos Sprague-Dawley
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:170526
[St] Status:MEDLINE
[do] DOI:10.1177/0022034517711146


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[PMID]:28495408
[Au] Autor:Hendek MK; Senses F; Kisa Ü; Aksoy N; Tekin U
[Ad] Endereço:Assistant Professor, Department of Periodontology, Faculty of Dentistry, Kirikkale University, Kirikkale, Turkey. Electronic address: mltmkrsyk@yahoo.com.
[Ti] Título:Is the Level of Nitric Oxide in the Dental Follicular Tissues of Impacted Third Molars With a History of Recurrent Pericoronitis a True Marker of Oxidative Stress?
[So] Source:J Oral Maxillofac Surg;75(10):2058-2062, 2017 Oct.
[Is] ISSN:1531-5053
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PURPOSE: Nitric oxide (NO) is an indicator of oxidative stress in several tissues. Its role in dental follicular (DF) tissues of impacted third molars with a history of recurrent pericoronitis is not well elucidated. The present study compared NO levels between inflamed and noninflamed DF tissues of impacted third molars with a history of recurrent pericoronitis. MATERIALS AND METHODS: A cross-sectional study was designed. The study sample included inflamed DF tissues (test group) with certain local inflammatory symptoms, such as pain, tenderness, swelling, and erythema and noninflamed DF tissues (control group) without local inflammatory symptoms of impacted mandibular third molars. Each patient contributed only 1 specimen to the samples. All tissues samples were biochemically investigated for NO levels as an indicator of oxidative stress. The primary predictor variable was inflammatory status; secondary predictor variables were age and gender. The primary outcome variable was NO level. Descriptive and comparative analyses were conducted. RESULTS: The test group consisted of 57 patients (28 men, 29 women; mean age, 23.28 ± 5.16 yr) and the control group consisted of 57 patients (30 men, 27 women; mean age, 23.02 ± 5.42 yr). No relevant intergroup differences were noted for demographic findings such as age and gender. NO levels were significantly higher in inflamed DF tissues of impacted third molars than in noninflamed DF tissues (P < .05). CONCLUSION: Results of this study showed that NO might be used as an indicator of oxidative stress and the necessity to remove impacted mandibular third molars with a history of recurrent pericoronitis.
[Mh] Termos MeSH primário: Saco Dentário/química
Saco Dentário/metabolismo
Dente Serotino
Óxido Nítrico/análise
Óxido Nítrico/biossíntese
Estresse Oxidativo
Pericoronite/metabolismo
Dente Impactado/metabolismo
[Mh] Termos MeSH secundário: Adolescente
Adulto
Biomarcadores/análise
Estudos Transversais
Feminino
Seres Humanos
Masculino
Recidiva
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 31C4KY9ESH (Nitric Oxide)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:AIM; D; IM
[Da] Data de entrada para processamento:170513
[St] Status:MEDLINE


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[PMID]:28259940
[Au] Autor:Zhang X; Du Y; Ling J; Li W; Liao Y; Wei X
[Ad] Endereço:Department of Prosthodontics, Guangdong Provincial Key Laboratory of Stomatology, Guanghua School of Stomatology, Sun Yat­sen University, Guangzhou, Guangdong 510055, P.R. China.
[Ti] Título:Dickkopf-related protein 3 negatively regulates the osteogenic differentiation of rat dental follicle cells.
[So] Source:Mol Med Rep;15(4):1673-1681, 2017 Apr.
[Is] ISSN:1791-3004
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:The present study aimed to investigate the effect of Dickkopf-related protein 3 (DKK3) on osteogenic differentiation of rat dental follicle cells (DFCs). A PCR array analysis of Wnt pathway activation in DFCs identified genes dysregulated by mineral induction. Among them, DKK3expression levels were decreased, and further experiments were conducted to investigate its role in DFC osteogenesis. By comparing DFCs grown in normal growth and mineral­induction media for 4 weeks, the present study confirmed that DKK3 was a potential target gene of osteogenesis through reverse transcription-quantitative polymerase chain reaction (RT­qPCR) and western blotting (WB). A short hairpin RNA (shRNA) was introduced into DFCs using a lentiviral vector to inhibit DKK3 expression. An alkaline phosphatase (ALP) activity assay and Alizarin Red staining were performed to observe the DKK3­shRNA DFCs. In addition, the osteogenic differentiation of DKK3­shRNA DFCs was analyzed by RT­qPCR and WB. In vivo, DKK3­shRNA DFCs seeded on hydroxyapatite/ß-tricalcium phosphate (HA/TCP) scaffolds were transplanted into the subcutaneous tissue of mice with severe combined immunodeficiency, followed by hematoxylin­eosin and Masson staining. The results confirmed that DKK3 expression was downregulated during mineral induction in rat DFCs. Lentivirus­mediated expression of DKK3 shRNA in DFCs promoted calcified­nodule formation, ALP activity and the expression of ß­catenin, runt­related transcription factor 2 and osteocalcin, compared with control cells. In vivo, the implanted section presented the majority of newly formed osteoid matrices and collagen, with limited space between the HA/TCP scaffolds and matrices. In conclusion, DKK3 expression negatively regulates the osteogenic differentiation of DFCs and, conversely, downregulation of DKK3 may enhance DFC osteogenesis.
[Mh] Termos MeSH primário: Diferenciação Celular
Saco Dentário/citologia
Saco Dentário/metabolismo
Peptídeos e Proteínas de Sinalização Intercelular/metabolismo
Osteogênese
[Mh] Termos MeSH secundário: Animais
Biomarcadores/metabolismo
Diferenciação Celular/efeitos dos fármacos
Células Cultivadas
Feminino
Masculino
Minerais/farmacologia
Osteogênese/efeitos dos fármacos
RNA Interferente Pequeno/metabolismo
Ratos Sprague-Dawley
Via de Sinalização Wnt/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Dkk3 protein, rat); 0 (Intercellular Signaling Peptides and Proteins); 0 (Minerals); 0 (RNA, Small Interfering)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170522
[Lr] Data última revisão:
170522
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170306
[St] Status:MEDLINE
[do] DOI:10.3892/mmr.2017.6165


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[PMID]:28199821
[Au] Autor:Desai RS; Momin YNA; Bansal S; Karjodkar FR
[Ad] Endereço:Professor and Head, Department of Oral Pathology, Nair Hospital Dental College, Mumbai, India. Electronic address: nansrd@hotmail.com.
[Ti] Título:Multiple Calcifying Hyperplastic Dental Follicles: A Case Report and Literature Review.
[So] Source:J Oral Maxillofac Surg;75(8):1702-1705, 2017 Aug.
[Is] ISSN:1531-5053
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Enlarged follicles associated with multiple unerupted teeth always comprise an area of considerable interest for oral and maxillofacial surgeons. The condition of multiple calcifying hyperplastic dental follicles is extremely rare and is characterized by multiple unerupted teeth with abundant calcifications and odontogenic epithelial rests in the enlarged dental follicles. We report an interesting case of multiple calcifying hyperplastic dental follicles in a 16-year-old healthy male patient.
[Mh] Termos MeSH primário: Calcinose/patologia
Saco Dentário/patologia
Dentição Permanente
Dente Decíduo/patologia
Dente não Erupcionado/patologia
[Mh] Termos MeSH secundário: Adolescente
Dente Canino/patologia
Cemento Dentário/patologia
Diagnóstico Diferencial
Seres Humanos
Hiperplasia
Masculino
Radiografia Panorâmica
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:AIM; D; IM
[Da] Data de entrada para processamento:170216
[St] Status:MEDLINE


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[PMID]:28116542
[Au] Autor:Morsczeck C; Reck A; Beck HC
[Ad] Endereço:Department of Cranio- and Maxillofacial Surgery, Hospital of the University of Regensburg, Franz-Josef-Strauss-Allee 11, 93053, Regensburg, Germany. christian.morsczeck@ukr.de.
[Ti] Título:The hedgehog-signaling pathway is repressed during the osteogenic differentiation of dental follicle cells.
[So] Source:Mol Cell Biochem;428(1-2):79-86, 2017 Apr.
[Is] ISSN:1573-4919
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Dental follicle stem cells (DFCs) are precursor cells of alveolar osteoblasts, and previous studies have shown that the growth factor bone morphogenetic protein (BMP)2 induces the osteogenic differentiation of DFCs. However, the molecular mechanism down-stream of the induction of the osteogenic differentiation by BMP2 remains elusive. We investigated therefore the phosphoproteome of DFCs after the induction of the osteogenic differentiation with BMP2. In this study, phosphoproteins of the hedgehog "off" state were differentially expressed. Further analyses revealed that BMP2 induced the expression of repressors of the hedgehog-signaling pathway such as Patched 1 (PTCH1), Suppressor of Fused (SUFU), and Parathyroid Hormone-Related Peptide (PTHrP). Previous studies suggested that hedgehog proteins induce the osteogenic differentiation of mesenchymal stem cells via a paracrine pathway. Indian hedgehog (IHH) induced the expression of the osteogenic transcription factor RUNX2. However, a supplementation of the BMP2-based osteogenic differentiation medium with IHH did not induce the expression of RUNX2. Moreover, IHH inhibited slightly the ALP activity and the mineralization of osteogenic-differentiated DFCs. In conclusion, our results suggest that BMP2 inhibits the hedgehog signaling after the induction of the osteogenic differentiation in DFCs.
[Mh] Termos MeSH primário: Proteína Morfogenética Óssea 2/farmacologia
Saco Dentário/citologia
Proteínas Hedgehog/metabolismo
Células-Tronco/citologia
[Mh] Termos MeSH secundário: Diferenciação Celular/efeitos dos fármacos
Células Cultivadas
Saco Dentário/metabolismo
Regulação da Expressão Gênica
Seres Humanos
Osteogênese/efeitos dos fármacos
Fosfoproteínas/metabolismo
Fosforilação
Proteômica/métodos
Transdução de Sinais/efeitos dos fármacos
Células-Tronco/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bone Morphogenetic Protein 2); 0 (Hedgehog Proteins); 0 (Phosphoproteins)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170125
[St] Status:MEDLINE
[do] DOI:10.1007/s11010-016-2918-4


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[PMID]:28087448
[Au] Autor:Ao C; Niu Y; Zhang X; He X; Zhang W; Lu C
[Ad] Endereço:State Key Laboratory of Polymer Materials Engineering, Polymer Research Institute at Sichuan University, Chengdu 610065, China.
[Ti] Título:Fabrication and characterization of electrospun cellulose/nano-hydroxyapatite nanofibers for bone tissue engineering.
[So] Source:Int J Biol Macromol;97:568-573, 2017 Apr.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Nanofibrous scaffolds from cotton cellulose and nano-hydroxyapatite (nano-HA) were electrospun for bone tissue engineering. The solution properties of cellulose/nano-HA spinning dopes and their associated electrospinnability were characterized. Morphological, thermal and mechanical properties of the electrospun cellulose/nano-HA nanocomposite nanofibers (ECHNN) were measured and the biocompatibility of ECHNN with human dental follicle cells (HDFCs) was evaluated. Scanning electron microscope (SEM) images indicated that the average diameter of ECHNN increased with a higher nano-HA loading and the fiber diameter distributions were well within the range of natural ECM (extra cellular matrix) fibers (50-500nm). The ECHNN exhibited extraordinary mechanical properties with a tensile strength and a Young's modulus up to 70.6MPa and 3.12GPa respectively. Moreover, it was discovered that the thermostability of the ECHNN could be enhanced with the incorporation of nano-HA. Cell culture experiments demonstrated that the ECHNN scaffolds were quite biocompatible for HDFCs attachment and proliferation, suggesting their great potentials as scaffold materials in bone tissue engineering.
[Mh] Termos MeSH primário: Materiais Biocompatíveis/química
Celulose/química
Durapatita/química
Eletricidade
Nanofibras/química
Engenharia Tecidual
Tecidos Suporte/química
[Mh] Termos MeSH secundário: Materiais Biocompatíveis/farmacologia
Adesão Celular/efeitos dos fármacos
Proliferação Celular/efeitos dos fármacos
Saco Dentário/citologia
Seres Humanos
Fenômenos Mecânicos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biocompatible Materials); 9004-34-6 (Cellulose); 91D9GV0Z28 (Durapatite)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170421
[Lr] Data última revisão:
170421
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170115
[St] Status:MEDLINE


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[PMID]:27888063
[Au] Autor:Chen X; Li S; Zeng Z; Gu Z; Yu Y; Zheng F; Zhou Y; Wang H
[Ad] Endereço:Department of Orthodontics, Hospital of Stomatology, Zhejiang University, Hangzhou, Zhejiang, China. Electronic address: cxp1979@163.com.
[Ti] Título:Notch1 signalling inhibits apoptosis of human dental follicle stem cells via both the cytoplasmic mitochondrial pathway and nuclear transcription regulation.
[So] Source:Int J Biochem Cell Biol;82:18-27, 2017 Jan.
[Is] ISSN:1878-5875
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Dental follicle stem cells (DFSCs) have been considered as promising candidate cells for periodontal tissue regeneration. Understanding the signalling pathways underlying the apoptosis of DFSCs will facilitate its biomedical application. Here we showed that Notch1 signalling could inhibit DFSCs apoptosis because the constitutive overexpression of the intracellular domain of Notch1 (ICN1) promoted proliferation and suppressed apoptosis by inhibiting cytoplasmic mitochondrial membrane depolarization, cytochrome c release and activation of caspase-9 and caspase-3. The survival-promoting effect of Notch1 was also accomplished by up-regulation of the anti-apoptotic proteins Bcl-2 and Mcl-1, down-regulation of the pro-apoptotic proteins Bax and Bad, and blockade of Bax multimerization. Moreover, p-Akt (S473) was significantly increased after ectopic Notch 1 activation. The expression of p53 was also inhibited in Notch1-overexpressing DFSCs, while the ectopic expression of p53 promoted apoptosis even when Notch1 was overexpressed. Meanwhile, all of the opposite phenomena were observed in Notch1 shRNA-silenced DFSCs. Our data strongly suggested that Notch1 signalling inhibited the apoptosis of DFSCs via the cytoplasmic mitochondrial pathway and ICN-Akt signalling pathway, together with nuclear gene expression regulation. These findings would provide molecular cues for the further medical application of DFSCs.
[Mh] Termos MeSH primário: Apoptose
Núcleo Celular/metabolismo
Saco Dentário/metabolismo
Regulação da Expressão Gênica
Receptor Notch1/agonistas
Transdução de Sinais
Células-Tronco/metabolismo
[Mh] Termos MeSH secundário: Adolescente
Biomarcadores/metabolismo
Proliferação Celular
Células Cultivadas
Saco Dentário/citologia
Feminino
Genes Reporter
Células HEK293
Seres Humanos
Células K562
Masculino
Fragmentos de Peptídeos/antagonistas & inibidores
Fragmentos de Peptídeos/genética
Fragmentos de Peptídeos/metabolismo
Domínios e Motivos de Interação entre Proteínas
Interferência de RNA
Receptor Notch1/antagonistas & inibidores
Receptor Notch1/genética
Receptor Notch1/metabolismo
Proteínas Recombinantes/química
Proteínas Recombinantes/metabolismo
Células-Tronco/citologia
Proteína Supressora de Tumor p53/genética
Proteína Supressora de Tumor p53/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (NOTCH1 protein, human); 0 (Peptide Fragments); 0 (Receptor, Notch1); 0 (Recombinant Proteins); 0 (TP53 protein, human); 0 (Tumor Suppressor Protein p53)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161127
[St] Status:MEDLINE


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Bolognese, Ana Maria
Texto completo
[PMID]:27764680
[Au] Autor:Lima RL; Holanda-Afonso RC; Moura-Neto V; Bolognese AM; DosSantos MF; Souza MM
[Ad] Endereço:Department of Orthodontics, Faculty of Dentistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil. Electronic address: rolopeslima@gmail.com.
[Ti] Título:Human dental follicle cells express embryonic, mesenchymal and neural stem cells markers.
[So] Source:Arch Oral Biol;73:121-128, 2017 Jan.
[Is] ISSN:1879-1506
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: This study was conducted to identify and characterize dental follicle stem cells (DFSCs) by analyzing expression of embryonic, mesenchymal and neural stem cells surface markers. Design Dental follicle cells (DFCs) were evaluated by immunocytochemistry using embryonic stem cells markers (OCT4 and SOX2), mesenchmal stem cells (MSCs) markers (Notch1, active Notch1, STRO, CD44, HLA-ABC, CD90), neural stem cells markers (Nestin and ß-III-tubulin), neural crest stem cells (NCSCs) markers (p75 and HNK1) and a glial cells marker (GFAP). RT-PCR was performed to identify the expression of OCT4 and NANOG in DFCs and dental follicle tissue. RESULTS: Immunocytochemistry and RT-PCR analysis revealed that a significant proportion of the DFCs evaluated expressed human embryonic stem cells marker OCT4 (75%) whereas NANOG was weakly expressed. A considerable amount of MSCs (90%) expressed Notch1, STRO, CD44 and HLA-ABC. However, they were weakly positive for CD90. Moreover, it was possible to demonstrate that dental follicle contains a significant proportion of neural stem/progenitors cells, expressing ß-III-tubulin (90%) and nestin (70%). Interestingly, immunocytochemistry showed DFCs positive for p75 (50%), HNK1 (<10%) and a small proportion (<20%) of GFAP-positive cells. This is the first study reporting the presence of NCSCs and glial-like cells in the dental follicle. CONCLUSIONS: The results of the present study suggest the occurrence of heterogeneous populations of stem cells, particularly neural stem/progenitor cells, in the dental follicle, Therefore, the human dental follicle might be a promising source of adult stem cells for regenerative purposes.
[Mh] Termos MeSH primário: Saco Dentário/citologia
Células Mesenquimais Estromais/metabolismo
Crista Neural/metabolismo
Células-Tronco Neurais/metabolismo
Proteínas/metabolismo
[Mh] Termos MeSH secundário: Adolescente
Técnicas de Cultura de Células
Voluntários Saudáveis
Seres Humanos
Imuno-Histoquímica
Microscopia Confocal
Reação em Cadeia da Polimerase em Tempo Real
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Proteins)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170324
[Lr] Data última revisão:
170324
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:161021
[St] Status:MEDLINE


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[PMID]:28317348
[Au] Autor:Liping W; Boqi L; Qi W; Xiaomin T; Yishan L
[Ad] Endereço:Dept. of Pediatric Dentistry and Preventive Dentistry, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830000, China.
[Ti] Título:[Expression and significance of wingless-type MMTV integration site family, member 5A/receptor tyrosine kinase-like orphan receptor 2 in the rat dental follicle].
[So] Source:Hua Xi Kou Qiang Yi Xue Za Zhi;34(4):341-345, 2016 Aug 01.
[Is] ISSN:1000-1182
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:OBJECTIVE: To observe the expression of wingless-type MMTV integration site family, member 5A (Wnt5A)/receptor tyrosine kinase-like orphan receptor 2 (Ror2) signal in the dental follicle cells during the normal eruption of the teeth as well as to explore the relationship between the expression of dental follicle cells and the formation of mature osteoclasts and eruption of the teeth. METHODS: The mandibulars of 1-13 d old SD rats were separated to observe the growth and develop-ment of the teeth and alveolar bone through hematoxylin-eosin(HE) staining. Ror2 and Wnt5A expressions in rat dental follicle were also observed through immunohistochemistry. Dental follicle cells from the lower first intact molar germs of 5-6-day old SD rats were separated and cultured. RESULTS: On the second day after birth, the dental follicle began to differentiate into periodontal tissues, but no obvious changes were observed in the alveolar bone one to three days after birth. On the fourth day, the number of osteoclasts increased significantly. The results of immunohistochemistry showed that Wnt5A was not significantly expressed in rat dental follicle tissues before the fourth day, but positive expression was expressed in the next day and continued to express to thirteenth days. Ror2 was expressed in the rat dental follicle at postnatal days 1-3, but weak expression was found in days 4-13. CONCLUSIONS: Wnt5A and Ror2 expressions in the process of tooth eruption have specific time distributions, suggesting that these expressions may participate in the regulation of the eruption of the teeth.
[Mh] Termos MeSH primário: Saco Dentário
[Mh] Termos MeSH secundário: Animais
Dente Molar
Osteoclastos
Periodonto
Ratos
Ratos Sprague-Dawley
Receptores Órfãos Semelhantes a Receptor Tirosina Quinase
Erupção Dentária
Proteínas Wnt
Proteína Wnt-5a
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (WNT5A protein, human); 0 (Wnt Proteins); 0 (Wnt-5a Protein); 0 (Wnt5a protein, rat); EC 2.7.10.1 (Receptor Tyrosine Kinase-like Orphan Receptors)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170403
[Lr] Data última revisão:
170403
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:170321
[St] Status:MEDLINE
[do] DOI:10.7518/hxkq.2016.04.004



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