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[PMID]:29023573
[Au] Autor:Wamboga C; Matovu E; Bessell PR; Picado A; Biéler S; Ndung'u JM
[Ad] Endereço:Ministry of Health, Kampala, Uganda.
[Ti] Título:Enhanced passive screening and diagnosis for gambiense human African trypanosomiasis in north-western Uganda - Moving towards elimination.
[So] Source:PLoS One;12(10):e0186429, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: The incidence of gambiense human African trypanosomiasis (gHAT) in Uganda has been declining, from 198 cases in 2008, to only 20 in 2012. Interruption of transmission of the disease by early diagnosis and treatment is core to the control and eventual elimination of gHAT. Until recently, the format of available screening tests had restricted screening and diagnosis to central health facilities (passive screening). We describe a novel strategy that is contributing to elimination of gHAT in Uganda through expansion of passive screening to the entire population at risk. METHODOLOGY / PRINCIPAL FINDINGS: In this strategy, patients who are clinically suspected of having gHAT at primary health facilities are screened using a rapid diagnostic test (RDT), followed by parasitological confirmation at strategically located microscopy centres. For patients who are positive with the RDT and negative by microscopy, blood samples undergo further testing using loop-mediated isothermal amplification (LAMP), a molecular test that detects parasite DNA. LAMP positive patients are considered strong suspects, and are re-evaluated by microscopy. Location and upgrading of facilities to perform microscopy and LAMP was informed by results of georeferencing and characterization of all public healthcare facilities in the 7 gHAT endemic districts in Uganda. Three facilities were upgraded to perform RDTs, microscopy and LAMP, 9 to perform RDTs and microscopy, and 200 to screen patients with RDTs. This reduced the distance that a sick person must travel to be screened for gHAT to a median distance of 2.5km compared to 23km previously. In this strategy, 9 gHAT cases were diagnosed in 2014, and 4 in 2015. CONCLUSIONS: This enhanced passive screening strategy for gHAT has enabled full coverage of the population at risk, and is being replicated in other gHAT endemic countries. The improvement in case detection is making elimination of the disease in Uganda an imminent possibility.
[Mh] Termos MeSH primário: Tripanossomíase Africana/diagnóstico
[Mh] Termos MeSH secundário: Buffy Coat/parasitologia
DNA de Protozoário/metabolismo
Instalações de Saúde
Seres Humanos
Incidência
Microscopia
Técnicas de Amplificação de Ácido Nucleico
Trypanosoma brucei gambiense/isolamento & purificação
Tripanossomíase Africana/epidemiologia
Tripanossomíase Africana/parasitologia
Uganda/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Protozoan)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171013
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186429


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[PMID]:28120426
[Au] Autor:van de Geer A; Gazendam RP; Tool AT; van Hamme JL; de Korte D; van den Berg TK; Zeerleder SS; Kuijpers TW
[Ad] Endereço:Department of Blood Cell Research, Sanquin Research, Amsterdam, The Netherlands.
[Ti] Título:Characterization of buffy coat-derived granulocytes for clinical use: a comparison with granulocyte colony-stimulating factor/dexamethasone-pretreated donor-derived products.
[So] Source:Vox Sang;112(2):173-182, 2017 Feb.
[Is] ISSN:1423-0410
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND AND OBJECTIVES: Buffy coat-derived granulocytes have been described as an alternative to the apheresis product from donors pretreated with dexamethasone and granulocyte colony-stimulating factor (G-CSF). The latter is - dependent on the local and national settings - obtained following a demanding and time-consuming procedure, which is undesirable in critically ill septic patients. In contrast, buffy coat-derived products have a large volume and are often heavily contaminated with red cells and platelets. We developed a new pooled buffy coat-derived product with high purity and small volume, and performed a comprehensive functional characterization of these granulocytes. MATERIALS AND METHODS: We pooled ten buffy coats following the production of platelet concentrates. Saline 0·9% was added to decrease the viscosity and the product was split into plasma, red cells and a 'super' buffy coat. Functional data of the granulocytes were compared to those obtained with granulocytes from healthy controls and G-CSF/dexamethasone-pretreated donors. RESULTS: Buffy coat-derived granulocytes showed adhesion, chemotaxis, reactive oxygen species production, degranulation, NETosis and in vitro killing of Staphylococcus aureus, Escherichia coli and Aspergillus species comparable to control and G-CSF/dexamethasone-derived granulocytes. Candida killing was superior compared to G-CSF/dexamethasone-derived granulocytes. Immunophenotyping was normal; especially no signs of activation in the buffy coat-derived granulocytes were seen. Viability was reduced. Buffy coats are readily available in the regular blood production process and would take away the concerns around the apheresis product. CONCLUSION: The product described appears a promising alternative for transfusion purposes.
[Mh] Termos MeSH primário: Buffy Coat/citologia
Dexametasona/farmacologia
Fator Estimulador de Colônias de Granulócitos/farmacologia
Granulócitos/efeitos dos fármacos
[Mh] Termos MeSH secundário: Adulto
Antígenos de Superfície/metabolismo
Remoção de Componentes Sanguíneos
Doadores de Sangue
Plaquetas/citologia
Adesão Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Quimiotaxia/efeitos dos fármacos
Granulócitos/citologia
Granulócitos/imunologia
Granulócitos/metabolismo
Seres Humanos
Imunofenotipagem
Contagem de Leucócitos
Masculino
NADPH Oxidases/metabolismo
Espécies Reativas de Oxigênio/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Surface); 0 (Reactive Oxygen Species); 143011-72-7 (Granulocyte Colony-Stimulating Factor); 7S5I7G3JQL (Dexamethasone); EC 1.6.3.- (NADPH Oxidases)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170126
[St] Status:MEDLINE
[do] DOI:10.1111/vox.12481


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[PMID]:27887865
[Au] Autor:Glovinski PV; Herly M; Mathiasen AB; Svalgaard JD; Borup R; Talman MM; Elberg JJ; Kølle ST; Drzewiecki KT; Fischer-Nielsen A
[Ad] Endereço:Department of Plastic Surgery, Breast Surgery & Burns, Copenhagen University Hospital Rigshospitalet, Copenhagen, Denmark. Electronic address: peter.viktor.glovinski.01@regionh.dk.
[Ti] Título:Overcoming the bottleneck of platelet lysate supply in large-scale clinical expansion of adipose-derived stem cells: A comparison of fresh versus three types of platelet lysates from outdated buffy coat-derived platelet concentrates.
[So] Source:Cytotherapy;19(2):222-234, 2017 Feb.
[Is] ISSN:1477-2566
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Platelet lysates (PL) represent a promising replacement for xenogenic growth supplement for adipose-derived stem cell (ASC) expansions. However, fresh platelets from human blood donors are not clinically feasible for large-scale cell expansion based on their limited supply. Therefore, we tested PLs prepared via three methods from outdated buffy coat-derived platelet concentrates (PCs) to establish an efficient and feasible expansion of ASCs for clinical use. METHODS: PLs were prepared by the freeze-thaw method from freshly drawn platelets or from outdated buffy coat-derived PCs stored in the platelet additive solution, InterSol. Three types of PLs were prepared from outdated PCs with platelets suspended in either (1) InterSol (not manipulated), (2) InterSol + supplemented with plasma or (3) plasma alone (InterSol removed). Using these PLs, we compared ASC population doubling time, cell yield, differentiation potential and cell surface markers. Gene expression profiles were analyzed using microarray assays, and growth factor concentrations in the cell culture medium were measured using enzyme-linked immunosorbent assay (ELISA). RESULTS: Of the three PL compositions produced from outdated PCs, removal of Intersol and resuspension in plasma prior to the first freezing process was overall the best. This specific outdated PL induced ASC growth kinetics, surface markers, plastic adherence and differentiation potentials comparable with PL from fresh platelets. ASCs expanded in PL from fresh versus outdated PCs exhibited different expressions of 17 overlapping genes, of which 10 were involved in cellular proliferation, although not significantly reflected by cell growth. Only minor differences in growth factor turnover were observed. CONCLUSION: PLs from outdated platelets may be an efficient and reliable source of human growth supplement allowing for large-scale ASC expansion for clinical use.
[Mh] Termos MeSH primário: Tecido Adiposo/citologia
Células-Tronco Adultas/citologia
Buffy Coat/citologia
Plaquetas/citologia
Preservação de Sangue/métodos
Técnicas de Cultura de Células/métodos
Extratos Celulares/provisão & distribuição
[Mh] Termos MeSH secundário: Adulto
Células-Tronco Adultas/fisiologia
Buffy Coat/transplante
Plaquetas/química
Coleta de Amostras Sanguíneas/métodos
Proliferação Celular
Separação Celular
Meios de Cultura/metabolismo
Feminino
Congelamento
Seres Humanos
Plasma/citologia
Transfusão de Plaquetas/métodos
Plasma Rico em Plaquetas/citologia
Refrigeração
Fatores de Tempo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cell Extracts); 0 (Culture Media)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170802
[Lr] Data última revisão:
170802
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161127
[St] Status:MEDLINE


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[PMID]:27628164
[Au] Autor:Tam IYS; Ng CW; Tam SY; Lau HYA
[Ad] Endereço:School of Biomedical Sciences, Faculty of Medicine, Chinese University of Hong Kong, Shatin, New Territories, Hong Kong.
[Ti] Título:Novel six-week protocol for generating functional human connective tissue-type (MC ) mast cells from buffy coats.
[So] Source:Inflamm Res;66(1):25-37, 2017 Jan.
[Is] ISSN:1420-908X
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: The aim of this study was to develop a novel protocol for generating large populations of fully mature and functional human mast cells (HMC) from CD34 hematopoietic stem cells which require less culturing time than previously reported methods. METHODS: CD34 cells isolated from fresh human buffy coats were sequentially cultured with different combinations of SCF, IL-6, IL-3, IL-9 and IL-4 under selected culturing conditions and time periods. Cells were then harvested for immunohistochemical characterization of morphological phenotypes and were functionally characterized by assessing their responses to IgE-dependent and -independent stimuli by measuring the release of inflammatory mediators and cytokines. Moreover, the pharmacological profiles of several classes of anti-inflammatory drugs in inhibiting the activation of these HMC were also characterized. RESULTS: We have developed a novel protocol that can generate large homogenous populations of mature and functional HMC in 6 weeks. These cells expressed both tryptase and chymase and were activated by anti-IgE, cationic peptides and calcium ionophores. Moreover, IgE-dependent activation of these cells was significantly inhibited by anti-inflammatory drugs. The morphological and functional characteristics of these mast cells resembled those of MC type or connective tissue-type HMC. DISCUSSION: Our protocol represents a novel time-saving and economical approach for generating large numbers of primary HMC for functional studies of mast cell biology and for profiling novel anti-inflammatory therapeutic agents with mast cell-inhibitory properties in humans.
[Mh] Termos MeSH primário: Buffy Coat/citologia
Técnicas de Cultura de Células
Mastócitos
[Mh] Termos MeSH secundário: Adulto
Anti-Inflamatórios/farmacologia
Anticorpos/farmacologia
Antígenos CD34
Movimento Celular
Células Cultivadas
Quimases/metabolismo
Tecido Conjuntivo
Células-Tronco Hematopoéticas/citologia
Liberação de Histamina
Seres Humanos
Imunoglobulina E/imunologia
Interleucina-8/metabolismo
Leucotrienos/metabolismo
Mastócitos/efeitos dos fármacos
Mastócitos/metabolismo
Mastócitos/fisiologia
Oxigênio
Prostaglandina D2/metabolismo
Triptases/metabolismo
Fator de Necrose Tumoral alfa/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Antibodies); 0 (Antigens, CD34); 0 (Interleukin-8); 0 (Leukotrienes); 0 (Tumor Necrosis Factor-alpha); 37341-29-0 (Immunoglobulin E); EC 3.4.21.39 (Chymases); EC 3.4.21.59 (Tryptases); RXY07S6CZ2 (Prostaglandin D2); S88TT14065 (Oxygen)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171114
[Lr] Data última revisão:
171114
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160916
[St] Status:MEDLINE
[do] DOI:10.1007/s00011-016-0989-z


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[PMID]:26939709
[Au] Autor:Grabmer C; Schlager S; Mayer G; Streif D; Lener T; Schallmoser K; Rohde E
[Ad] Endereço:Department for Blood Group Serology and Transfusion Medicine, SALK - Paracelsus Medical University (PMU), Salzburg, Austria.
[Ti] Título:An alternative mini buffy coat preparation method for adult patients with extracorporeal photopheresis contraindications.
[So] Source:J Clin Apher;32(1):12-15, 2017 Feb.
[Is] ISSN:1098-1101
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Extracorporeal photopheresis (ECP) is an important cell-based therapy for various diseases but is limited to patients eligible for apheresis. We developed an alternative mini buffy coat (BC) preparation method using the Spectra Optia® apheresis system and compared its efficacy of white blood cell (WBC) recovery with the standard mini BC preparation method already established for pediatric patients. METHODS: Whole blood (450 ± 45 mL) samples were collected from 30 randomly selected healthy volunteer blood donors and divided into two groups. In the first group, WBCs were separated with a fully automated separator device (Compomat G4 ). In the second group, BCs were separated with the bone marrow processing program of the Spectra Optia apheresis system. RESULTS: There were no significant differences in total leukocyte counts per product between the two groups. In contrast, lymphocyte counts per product were significantly higher (P < 0.001) in BCs separated from apheresis. CONCLUSION: Our novel technique resulted in similar WBC yields but higher lymphocyte yields than the standard mini BC preparation method. This method can serve as an alternative to WBC collection in conventional ECP for adult patients with apheresis contraindications. J. Clin. Apheresis 32:12-15, 2017. © 2016 Wiley Periodicals, Inc.
[Mh] Termos MeSH primário: Buffy Coat/citologia
Fotoferese/métodos
[Mh] Termos MeSH secundário: Adulto
Remoção de Componentes Sanguíneos/métodos
Remoção de Componentes Sanguíneos/normas
Separação Celular/métodos
Seres Humanos
Contagem de Leucócitos
Leucócitos/citologia
Contagem de Linfócitos
Fotoferese/normas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170309
[Lr] Data última revisão:
170309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160305
[St] Status:MEDLINE
[do] DOI:10.1002/jca.21455


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[PMID]:27882772
[Au] Autor:Lizarraga D; Huen K; Combs M; Escudero-Fung M; Eskenazi B; Holland N
[Ad] Endereço:School of Public Health, Center for Environmental Research on Children's Health (CERCH), University of California, Berkeley, CA 94720, USA.
[Ti] Título:miRNAs differentially expressed by next-generation sequencing in cord blood buffy coat samples of boys and girls.
[So] Source:Epigenomics;8(12):1619-1635, 2016 Dec.
[Is] ISSN:1750-192X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:AIM: Differences in children's development and susceptibility to diseases and exposures have been observed by sex, yet human studies of sex differences in miRNAs are limited. MATERIALS & METHODS: The genome-wide miRNA expression was characterized by sequencing-based EdgeSeq assay in cord blood buffy coats from 89 newborns, and 564 miRNAs were further analyzed. RESULTS: Differential expression of most miRNAs was higher in boys. Neurodevelopment, RNA metabolism and metabolic ontology terms were enriched among miRNA targets. The majority of upregulated miRNAs (86%) validated by nCounter maintained positive-fold change values; however, only 21% reached statistical significance by false discovery rate. CONCLUSION: Accounting for host factors like sex may improve the sensitivity of epigenetic analyses for epidemiological studies in early childhood.
[Mh] Termos MeSH primário: MicroRNAs/genética
[Mh] Termos MeSH secundário: Buffy Coat/metabolismo
Feminino
Sangue Fetal/metabolismo
Seres Humanos
Recém-Nascido
Masculino
Análise de Sequência de RNA
Fatores Sexuais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (MicroRNAs)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161125
[St] Status:MEDLINE


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[PMID]:27658589
[Au] Autor:Mohapatra S; Ghosh A; Singh R; Singh DP; Sharma B; Samantaray JC; Deb M; Gaind R
[Ad] Endereço:Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India.
[Ti] Título:Hemozoin Pigment: An Important Tool for Low Parasitemic Malarial Diagnosis.
[So] Source:Korean J Parasitol;54(4):393-7, 2016 Aug.
[Is] ISSN:1738-0006
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:Low parasitemic condition in malaria remains a diagnostic challenge; as the available diagnostic methods failed to detect. Currently, hemozoin (Hz) pigment is gaining attention in the diagnosis of malaria. The major drawback is ease of detection of Hz in routine practice. A pilot study was conducted to evaluate the role of Hz pigment and to compare the performance of quantitative buffy coat assay (QBC) and PCR in such conditions. Clinically suspected cases of malaria were examined by both Giemsa stain and immunochromatographic test (ICT). Samples positive by ICT and negative by Giemsa stain were further examined by nested PCR targeting 18S rRNA and QBC for the presence of malaria parasites and pigments. Thirty blood samples fulfilled the inclusion criteria out of which 23 were Plasmodium vivax (Pv), 4 Plasmodium falciparum (Pf), and 3 mixed (Pv and Pf) by immunochromatographic test. Twenty-one out of 30 (70%) were positive by nested PCR in comparison to 25/30 (83%) by QBC. Samples containing both malaria parasites and Hz pigment by QBC completely showed concordance with the PCR result. However, 61% of total samples containing only Hz pigment were observed positive by PCR. Hz pigment remains an important tool for malaria diagnosis. Identification of leukocytes containing pigments by QBC not only indicates recent malarial infections but also puts light on severity of the disease. QBC assay is a rapid, highly sensitive, and cost-effective method to detect malaria parasites and Hz pigment especially in low parasitemic conditions.
[Mh] Termos MeSH primário: Buffy Coat/química
Buffy Coat/parasitologia
Hemeproteínas/análise
Imunocromatografia/métodos
Malária/diagnóstico
Reação em Cadeia da Polimerase/métodos
RNA de Protozoário/genética
[Mh] Termos MeSH secundário: Seres Humanos
Projetos Piloto
RNA Ribossômico 18S/genética
Sensibilidade e Especificidade
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hemeproteins); 0 (RNA, Protozoan); 0 (RNA, Ribosomal, 18S); 39404-00-7 (hemozoin)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170404
[Lr] Data última revisão:
170404
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160924
[St] Status:MEDLINE
[do] DOI:10.3347/kjp.2016.54.4.393


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[PMID]:27526671
[Au] Autor:Crimmins D; Flanagan P; Charlewood R; Ruggiero K
[Ad] Endereço:Clinical Components Development, New Zealand Blood Service, Auckland, New Zealand.
[Ti] Título:In vitro comparison between gamma-irradiated cryopreserved and Day 7 liquid-stored buffy coat-derived platelet components.
[So] Source:Transfusion;56(11):2799-2807, 2016 Nov.
[Is] ISSN:1537-2995
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Cryopreserved platelet (PLT) components stored at -80°C in 5% to 6% dimethyl sulfoxide (DMSO) demonstrate enhanced hemostatic activity. Alterations in PLT surface glycoprotein expression and release of procoagulant microparticles during the freeze/thaw cycle result in PLT activation. Nothing is known of the effect of gamma irradiation on the in vitro quality of reconstituted cryopreserved PLTs. STUDY DESIGN AND METHODS: Gamma-irradiated (25-50 Gy) buffy coat-derived PLT components were either stored at room temperature for 7 days (the current expiry in New Zealand) or cryopreserved at -80°C using 5% to 6% DMSO. Cryopreserved PLTs were thawed at 37°C and reconstituted in ABO-identical plasma or PAS-E and compared to Day 7 gamma-irradiated liquid-stored PLTs. In vitro assays were performed to assess glycoprotein expression, PLT functionality and soluble cytokine release. RESULTS: Cryopreserved PLTs after thawing and reconstitution in ABO-matched plasma or PAS-E displayed differing recoveries (82.7 and 75.9%, respectively). Key expression levels of glycoproteins GPIbα (CD42b) and GPIIb (CD41a) were reduced. Cryopreserved PLTs retained the ability to form an effective functional clot, while showing accelerated initiation of clot formation (R-time) compared to Day 7 gamma-irradiated liquid-stored PLTs. CONCLUSION: Gamma-irradiated buffy coat-derived liquid-stored and cryopreserved PLTs have distinctly differing phenotypes. Cryopreserved PLTs reconstituted in ABO plasma have enhanced clot strength driven by coagulation factors and fibrinogen levels not present in PAS-E. Irradiated cryopreserved PLTs maintain a similar in vitro quality profile and hemostatic behavior to previously published, nonirradiated cryopreserved PLTs.
[Mh] Termos MeSH primário: Plaquetas/efeitos da radiação
Preservação de Sangue/métodos
Raios gama
[Mh] Termos MeSH secundário: Sistema do Grupo Sanguíneo ABO
Buffy Coat/efeitos da radiação
Preservação de Sangue/normas
Criopreservação/métodos
Criopreservação/normas
Seres Humanos
Complexo Glicoproteico GPIb-IX de Plaquetas/metabolismo
Glicoproteína IIb da Membrana de Plaquetas/metabolismo
Temperatura Ambiente
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (ABO Blood-Group System); 0 (Platelet Glycoprotein GPIb-IX Complex); 0 (Platelet Membrane Glycoprotein IIb)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170525
[Lr] Data última revisão:
170525
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160817
[St] Status:MEDLINE
[do] DOI:10.1111/trf.13763


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[PMID]:27432557
[Au] Autor:Taha M; Kalab M; Yi QL; Maurer E; Jenkins C; Schubert P; Ramirez-Arcos S
[Ad] Endereço:Canadian Blood Services, Ottawa, ON, Canada.
[Ti] Título:Bacterial survival and distribution during buffy coat platelet production.
[So] Source:Vox Sang;111(4):333-340, 2016 Nov.
[Is] ISSN:1423-0410
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND AND OBJECTIVES: At Canadian Blood Services, buffy coat (BC) platelet concentrates (BC-PCs) show a generally lower bacterial contamination rate than apheresis PCs. This study investigated whether the PC production method contributes to this observation. MATERIALS AND METHODS: Whole blood (WB) inoculated with eight bacterial strains was processed using the BC method. Bacteria were enumerated throughout BC-PC production and subsequent PC storage. Endotoxin production and bacterial adhesion to PC bags were evaluated during PC storage. PC quality was monitored by CD62P expression (flow cytometry) and changes in dynamic light scattering (ThromboLUX ). RESULTS: During overnight WB hold, Staphylococcus epidermidis titres remained unchanged, commercial Escherichia coli and Klebsiella pneumoniae were eliminated and the remaining organisms proliferated to high concentrations. Through BC-PC production, bacteria segregated preferentially towards the cellular fractions compared to plasma (P < 0·05). During PC storage, most bacteria adhered to the PC bags and Gram negatives produced clinically significant endotoxin levels. Changes in CD62P expression or ThromboLUX scoring did not consistently reflect bacterial contamination in BC-PCs. CONCLUSION: WB hold during BC-PC production does not have a broad-spectrum bactericidal effect, and therefore, other factors contribute to low rates of contamination in BC-PCs.
[Mh] Termos MeSH primário: Plaquetas/microbiologia
Segurança do Sangue
Plasma Rico em Plaquetas/microbiologia
[Mh] Termos MeSH secundário: Buffy Coat/microbiologia
Plaquetas/metabolismo
Escherichia coli/fisiologia
Citometria de Fluxo
Seres Humanos
Klebsiella pneumoniae/fisiologia
Viabilidade Microbiana
Selectina-P/metabolismo
Plaquetoferese
Serratia marcescens/fisiologia
Staphylococcus epidermidis/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (P-Selectin); 0 (SELP protein, human)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170116
[Lr] Data última revisão:
170116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160720
[St] Status:MEDLINE
[do] DOI:10.1111/vox.12427


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[PMID]:27071934
[Au] Autor:Ryder JR; O'Connell MJ; Rudser KD; Fox CK; Solovey AN; Hebbel RP; Kelly AS
[Ad] Endereço:Department of Pediatrics, University of Minnesota Medical School, Minneapolis, MN 55455, USA.
[Ti] Título:Reproducibility of circulating endothelial cell enumeration and activation in children and adolescents.
[So] Source:Biomark Med;10(5):463-71, 2016 May.
[Is] ISSN:1752-0371
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: We examined the reproducibility of circulating endothelial cells (CEC) enumeration and activation among youth. MATERIALS AND METHODS: CECs from 151 youth were measured at baseline and 1 week follow-up. Enumeration of CEC in fresh whole blood was determined by direct assessment of buffy coat smears (CD146+ nucleated cells) and activated CEC (%VCAM-1 expression) was determined after immunomagnetic enrichment and co-staining of nuclei, plus positivity for P1H12 and VCAM-1. RESULTS: No statistically significant difference in CEC enumeration (1.2 ± 2.5 vs 1.3 ± 2.2 CEC/milliliter of whole blood, p = 0.745) or activated CEC (57.1 ± 24.4 vs 58.0 ± 21.3 %VCAM-1, p = 0.592) between baseline and 1 week follow-up. CONCLUSION: On a cohort basis, CEC enumeration and activation are reproducible in youth. Relatively high individual biological variability may limit its clinical utility.
[Mh] Termos MeSH primário: Biomarcadores/sangue
Células Endoteliais/citologia
[Mh] Termos MeSH secundário: Adolescente
Buffy Coat/citologia
Índice de Massa Corporal
Antígeno CD146/metabolismo
Criança
Células Endoteliais/metabolismo
Feminino
Citometria de Fluxo
Seguimentos
Seres Humanos
Masculino
Obesidade/metabolismo
Obesidade/patologia
Fenótipo
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (CD146 Antigen)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160414
[St] Status:MEDLINE
[do] DOI:10.2217/bmm-2015-0051



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