Base de dados : MEDLINE
Pesquisa : A16 [Categoria DeCS]
Referências encontradas : 199 [refinar]
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  1 / 199 MEDLINE  
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[PMID]:17908929
[Au] Autor:Srinivasan RS; Dillard ME; Lagutin OV; Lin FJ; Tsai S; Tsai MJ; Samokhvalov IM; Oliver G
[Ad] Endereço:Department of Genetics and Tumor Cell Biology, St. Jude Children's Hospital, Memphis, Tennessee 38105, USA.
[Ti] Título:Lineage tracing demonstrates the venous origin of the mammalian lymphatic vasculature.
[So] Source:Genes Dev;21(19):2422-32, 2007 Oct 01.
[Is] ISSN:0890-9369
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The origin of the mammalian lymphatic vasculature has been debated for more than 100 years. Whether lymphatic endothelial cells have a single or dual, venous or mesenchymal origin remains controversial. To resolve this debate, we performed Cre/loxP-based lineage-tracing studies using mouse strains expressing Cre recombinase under the control of the Tie2, Runx1, or Prox1 promoter elements. These studies, together with the analysis of Runx1-mutant embryos lacking definitive hematopoiesis, conclusively determined that from venous-derived lymph sacs, lymphatic endothelial cells sprouted, proliferated, and migrated to give rise to the entire lymphatic vasculature, and that hematopoietic cells did not contribute to the developing lymph sacs. We conclude that the mammalian lymphatic system has a solely venous origin.
[Mh] Termos MeSH primário: Linhagem da Célula
Células Endoteliais/citologia
Vasos Linfáticos/embriologia
Veias/citologia
[Mh] Termos MeSH secundário: Animais
Movimento Celular
Proliferação Celular
Subunidade alfa 2 de Fator de Ligação ao Core/análise
Subunidade alfa 2 de Fator de Ligação ao Core/genética
Estruturas Embrionárias/química
Estruturas Embrionárias/citologia
Estruturas Embrionárias/efeitos dos fármacos
Células Endoteliais/fisiologia
Células-Tronco Hematopoéticas/fisiologia
Proteínas de Homeodomínio/análise
Proteínas de Homeodomínio/genética
Integrases/genética
Camundongos
Camundongos Transgênicos
Receptor TIE-2/análise
Receptor TIE-2/genética
Tamoxifeno/farmacologia
Proteínas Supressoras de Tumor/análise
Proteínas Supressoras de Tumor/genética
Veias/embriologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Core Binding Factor Alpha 2 Subunit); 0 (Homeodomain Proteins); 0 (Tumor Suppressor Proteins); 0 (prospero-related homeobox 1 protein); 094ZI81Y45 (Tamoxifen); EC 2.7.10.1 (Receptor, TIE-2); EC 2.7.7.- (Cre recombinase); EC 2.7.7.- (Integrases)
[Em] Mês de entrada:0711
[Cu] Atualização por classe:161019
[Lr] Data última revisão:
161019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:071003
[St] Status:MEDLINE


  2 / 199 MEDLINE  
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[PMID]:17850793
[Au] Autor:Kato S; Mohri Y; Matsuo T; Ogawa E; Umezawa A; Okuyama R; Nishimori K
[Ad] Endereço:Laboratory of Molecular Biology, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan.
[Ti] Título:Eye-open at birth phenotype with reduced keratinocyte motility in LGR4 null mice.
[So] Source:FEBS Lett;581(24):4685-90, 2007 Oct 02.
[Is] ISSN:0014-5793
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:We observed a consistent eye-open at birth (EOB) phenotype in mouse pups homozygous for a leucine-rich repeat containing G-protein coupled receptor 4 (Lgr4) allele deleting the whole transmembrane domain coding region. An in vitro wound-healing scratch assay showed notably reduced keratinocyte motility in the null mice. Phalloidin staining of F-actin in the eyelid epidermis was also reduced. We also generated keratinocyte-specific Lgr4 deficient mice, circumventing the embryonic/neonatal lethality and kidney abnormalities. Most of the conditional Lgr4 knockout mice showed the EOB phenotype. Thus, Lgr4 might be a novel gene class regulating cell motility.
[Mh] Termos MeSH primário: Anormalidades do Olho/genética
Anormalidades do Olho/fisiopatologia
Olho/fisiopatologia
Queratinócitos/citologia
Queratinócitos/metabolismo
Receptores Acoplados a Proteínas-G/deficiência
Receptores Acoplados a Proteínas-G/metabolismo
[Mh] Termos MeSH secundário: Animais
Animais Recém-Nascidos
Sobrevivência Celular
Células Cultivadas
Estruturas Embrionárias/embriologia
Estruturas Embrionárias/metabolismo
Olho/embriologia
Olho/metabolismo
Regulação da Expressão Gênica no Desenvolvimento
Camundongos
Camundongos Knockout
Fenótipo
Receptores Acoplados a Proteínas-G/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (LGR4 protein, mouse); 0 (Receptors, G-Protein-Coupled)
[Em] Mês de entrada:0711
[Cu] Atualização por classe:070924
[Lr] Data última revisão:
070924
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:070914
[St] Status:MEDLINE


  3 / 199 MEDLINE  
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[PMID]:17846422
[Au] Autor:Magnúsdóttir E; Kalachikov S; Mizukoshi K; Savitsky D; Ishida-Yamamoto A; Panteleyev AA; Calame K
[Ad] Endereço:Department of Biological Sciences, Columbia University, New York, NY 10027, USA.
[Ti] Título:Epidermal terminal differentiation depends on B lymphocyte-induced maturation protein-1.
[So] Source:Proc Natl Acad Sci U S A;104(38):14988-93, 2007 Sep 18.
[Is] ISSN:0027-8424
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The cornified layer is a compacted lattice of lipid-embedded corneocytes that provides an organism's barrier to the external environment. Cornification is the final differentiative step for epidermal keratinocytes and involves dramatic cell condensation before death. Using conditional gene deletion in mice, we identified the transcriptional repressor Blimp-1 (B lymphocyte-induced maturation protein-1) as an important regulator of keratinocyte transition from the granular to the cornified layer. More than 250 genes are misregulated in conditional knockout epidermis, including those encoding transcription factors, signal transduction components, proteinases, and enzymes involved in lipid metabolism. Steady-state mRNA and ChIP analyses of a subset of these genes provide evidence that nfat5, fos, prdm1, and dusp16 are novel direct targets of Blimp-1. Identifying nfat5 as a target of Blimp-1 repression indicates that cornification involves suppression of normal osmotic regulation in granular cells. Consistently, conditional knockout mice have delayed barrier formation as embryos, enlarged granular layer cells and corneocytes, and a morphologically abnormal cornified layer. These studies provide insight into cornification, identifying transcriptional regulatory circuitry and indicating the importance of blocking osmotic homeostasis.
[Mh] Termos MeSH primário: Diferenciação Celular
Epiderme/citologia
Epiderme/embriologia
Proteínas Repressoras/metabolismo
Fatores de Transcrição/metabolismo
[Mh] Termos MeSH secundário: Animais
Fosfatases de Especificidade Dupla/genética
Fosfatases de Especificidade Dupla/metabolismo
Estruturas Embrionárias/metabolismo
Epiderme/metabolismo
Deleção de Genes
Seres Humanos
Queratinócitos/metabolismo
Camundongos
Camundongos Knockout
Fosfatases da Proteína Quinase Ativada por Mitógeno/genética
Fosfatases da Proteína Quinase Ativada por Mitógeno/metabolismo
Fatores de Transcrição NFATC/genética
Fatores de Transcrição NFATC/metabolismo
Análise de Sequência com Séries de Oligonucleotídeos
Fenótipo
Fator 1 de Ligação ao Domínio I Regulador Positivo
Proteínas Proto-Oncogênicas c-fos/genética
Proteínas Proto-Oncogênicas c-fos/metabolismo
RNA Mensageiro/metabolismo
Proteínas Repressoras/genética
Fatores de Transcrição/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (NFATC Transcription Factors); 0 (Prdm1 protein, mouse); 0 (Proto-Oncogene Proteins c-fos); 0 (RNA, Messenger); 0 (Repressor Proteins); 0 (Transcription Factors); 138415-26-6 (PRDM1 protein, human); EC 2.1.1.- (Positive Regulatory Domain I-Binding Factor 1); EC 3.1.3.16 (Mitogen-Activated Protein Kinase Phosphatases); EC 3.1.3.48 (DUSP16 protein, human); EC 3.1.3.48 (Dual-Specificity Phosphatases)
[Em] Mês de entrada:0711
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:070912
[St] Status:MEDLINE


  4 / 199 MEDLINE  
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[PMID]:17846168
[Au] Autor:Benetti R; Gonzalo S; Jaco I; Schotta G; Klatt P; Jenuwein T; Blasco MA
[Ad] Endereço:Telomeres and Telomerase Group, Molecular Oncology Program, Spanish National Cancer Centre, Madrid, Spain.
[Ti] Título:Suv4-20h deficiency results in telomere elongation and derepression of telomere recombination.
[So] Source:J Cell Biol;178(6):925-36, 2007 Sep 10.
[Is] ISSN:0021-9525
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mammalian telomeres have heterochromatic features, including trimethylated histone H3 at lysine 9 (H3K9me3) and trimethylated histone H4 at lysine 20 (H4K20me3). In addition, subtelomeric DNA is hypermethylated. The enzymatic activities responsible for these modifications at telomeres are beginning to be characterized. In particular, H4K20me3 at telomeres could be catalyzed by the novel Suv4-20h1 and Suv4-20h2 histone methyltransferases (HMTases). In this study, we demonstrate that the Suv4-20h enzymes are responsible for this histone modification at telomeres. Cells deficient for Suv4-20h2 or for both Suv4-20h1 and Suv4-20h2 show decreased levels of H4K20me3 at telomeres and subtelomeres in the absence of changes in H3K9me3. These epigenetic alterations are accompanied by telomere elongation, indicating a role for Suv4-20h HMTases in telomere length control. Finally, cells lacking either the Suv4-20h or Suv39h HMTases show increased frequencies of telomere recombination in the absence of changes in subtelomeric DNA methylation. These results demonstrate the importance of chromatin architecture in the maintenance of telomere length homeostasis and reveal a novel role for histone lysine methylation in controlling telomere recombination.
[Mh] Termos MeSH primário: Metilação de DNA
Histona-Lisina N-Metiltransferase/metabolismo
Recombinação Genética
Telômero/metabolismo
[Mh] Termos MeSH secundário: Animais
Cromossomos de Mamíferos/genética
Cromossomos de Mamíferos/metabolismo
Células-Tronco Embrionárias/metabolismo
Estruturas Embrionárias/citologia
Epigênese Genética
Fibroblastos/metabolismo
Histona-Lisina N-Metiltransferase/genética
Histonas/genética
Histonas/metabolismo
Técnicas In Vitro
Metilação
Camundongos
Telômero/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Histones); EC 2.1.1.43 (Histone-Lysine N-Methyltransferase)
[Em] Mês de entrada:0711
[Cu] Atualização por classe:141120
[Lr] Data última revisão:
141120
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:070912
[St] Status:MEDLINE


  5 / 199 MEDLINE  
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[PMID]:17825046
[Au] Autor:Ruhul Amin AR; Uddin Biswas MH; Senga T; Feng GS; Kannagi R; Agarwal ML; Hamaguchi M
[Ad] Endereço:Division of Cancer Biology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan.
[Ti] Título:A role for SHPS-1/SIRPalpha in Concanavalin A-dependent production of MMP-9.
[So] Source:Genes Cells;12(9):1023-33, 2007 Sep.
[Is] ISSN:1356-9597
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:SHPS-1/SIRPalpha1 is a transmembrane glycoprotein that belongs to the immunoglobulin (Ig) super family. In the present study, we show that SHPS-1 strongly associates with Concanavalin A (Con A), a plant lectin obtained from jack beans. Further studies with SHPS-1 mutants reveal that the extracellular domain of SHPS-1 containing the Ig sequence is responsible for its association with Con A. Con A treatment induces cross-linking and multimerization of the SHPS-1 protein in the plasma membrane, accompanied by its tyrosine phosphorylation and recruitment of SHP-2. In contrast, Ricinus communis agglutinin (RCA), another lectin obtained from castor bean, does not bind or activate tyrosine phosphorylation of SHPS-1. Moreover, Con A activates Akt in a SHP-2-dependent manner. Treatment of mouse embryonic fibroblasts (MEFs) with Con A induces secretion of matrix metalloproteinase (MMP)-9, a phenomenon that is inhibited in cells expressing YF mutant of SHPS-1, a dominant negative form of Akt or in cells pre-treated with an Akt inhibitor, LY294002 or extracellular-signal regulated kinase (Erk) inhibitor, U0126. In addition, expression of the YF mutant of SHPS-1 inhibits Con A-dependent activation of Akt and Erk kinases. Taken together, our results suggest that SHPS-1 is a receptor for Con A that mediates Con A-dependent MMP-9 secretion through SHP-2-promoted activation of both Akt and Erk pathways.
[Mh] Termos MeSH primário: Concanavalina A/farmacologia
Metaloproteinase 9 da Matriz/biossíntese
Receptores Imunológicos/fisiologia
[Mh] Termos MeSH secundário: Animais
Sítios de Ligação
Células COS
Membrana Celular/metabolismo
Células Cultivadas
Cercopithecus aethiops
Concanavalina A/metabolismo
Estruturas Embrionárias/metabolismo
MAP Quinases Reguladas por Sinal Extracelular/metabolismo
Fibroblastos/metabolismo
Camundongos
Fosforilação
Lectinas de Plantas/metabolismo
Estrutura Terciária de Proteína
Proteína Tirosina Fosfatase não Receptora Tipo 11/metabolismo
Proteínas Proto-Oncogênicas c-akt/metabolismo
Receptores Imunológicos/genética
Transdução de Sinais
Transfecção
Tirosina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Plant Lectins); 0 (Ptpns1 protein, mouse); 0 (Receptors, Immunologic); 0 (Ricinus communis agglutinin-1); 11028-71-0 (Concanavalin A); 42HK56048U (Tyrosine); EC 2.7.11.1 (Proto-Oncogene Proteins c-akt); EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases); EC 3.1.3.48 (Protein Tyrosine Phosphatase, Non-Receptor Type 11); EC 3.1.3.48 (Ptpn11 protein, mouse); EC 3.4.24.35 (Matrix Metalloproteinase 9)
[Em] Mês de entrada:0711
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:070911
[St] Status:MEDLINE


  6 / 199 MEDLINE  
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[PMID]:17804628
[Au] Autor:Pasqualetti M; Díaz C; Renaud JS; Rijli FM; Glover JC
[Ad] Endereço:Institut de Génétique et de Biologie Moléculaire et Cellulaire, Centre National de la Recherche Scientifique/Inserm/Université Louis Pasteur, Unité Mixte de Recherche 7104, Commanaute Urbaine de Strasbourg, 67404 Illkirch Cedex, France.
[Ti] Título:Fate-mapping the mammalian hindbrain: segmental origins of vestibular projection neurons assessed using rhombomere-specific Hoxa2 enhancer elements in the mouse embryo.
[So] Source:J Neurosci;27(36):9670-81, 2007 Sep 05.
[Is] ISSN:1529-2401
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:As a step toward generating a fate map of identified neuron populations in the mammalian hindbrain, we assessed the contributions of individual rhombomeres to the vestibular nuclear complex, a major sensorimotor area that spans the entire rhombencephalon. Transgenic mice harboring either the lacZ or the enhanced green fluorescent protein reporter genes under the transcriptional control of rhombomere-specific Hoxa2 enhancer elements were used to visualize rhombomere-derived domains. We labeled functionally identifiable vestibular projection neuron groups retrogradely with conjugated dextran-amines at successive embryonic stages and obtained developmental fate maps through direct comparison with the rhombomere-derived domains in the same embryos. The fate maps show that each vestibular neuron group derives from a unique rostrocaudal domain that is relatively stable developmentally, suggesting that anteroposterior migration is not a major contributor to the rostrocaudal patterning of the vestibular system. Most of the groups are multisegmental in origin, and each rhombomere is fated to give rise to two or more vestibular projection neuron types, in a complex pattern that is not segmentally iterated. Comparison with studies in the chicken embryo shows that the rostrocaudal patterning of identified vestibular projection neuron groups is generally well conserved between avians and mammalians but that significant species-specific differences exist in the rostrocaudal limits of particular groups. This mammalian hindbrain fate map can be used as the basis for targeting genetic manipulation to specific subpopulations of vestibular projection neurons.
[Mh] Termos MeSH primário: Estruturas Embrionárias/embriologia
Elementos Facilitadores Genéticos/genética
Proteínas de Homeodomínio/genética
Neurônios/citologia
Rombencéfalo/embriologia
Núcleos Vestibulares/embriologia
[Mh] Termos MeSH secundário: Animais
Linhagem da Célula
Embrião de Mamíferos
Estruturas Embrionárias/citologia
Genes Reporter
Camundongos
Camundongos Transgênicos
Neurônios/classificação
Neurônios/metabolismo
Rombencéfalo/citologia
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Homeodomain Proteins); 0 (Hoxa2 protein, mouse)
[Em] Mês de entrada:0710
[Cu] Atualização por classe:081121
[Lr] Data última revisão:
081121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:070907
[St] Status:MEDLINE


  7 / 199 MEDLINE  
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[PMID]:17761889
[Au] Autor:Waclawik A; Ziecik AJ
[Ad] Endereço:Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Tuwima 10, 10-747 Olsztyn, Poland.
[Ti] Título:Differential expression of prostaglandin (PG) synthesis enzymes in conceptus during peri-implantation period and endometrial expression of carbonyl reductase/PG 9-ketoreductase in the pig.
[So] Source:J Endocrinol;194(3):499-510, 2007 Sep.
[Is] ISSN:0022-0795
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Prostaglandins (PGs) play a pivotal role in luteolysis, maternal recognition of pregnancy, and implantation. In many species, including pigs, both conceptus (embryo and associated membranes) and endometrium synthesize PGE(2), which may antagonize PGF(2alpha) by playing a luteotropic/antiluteolytic role. Previously, we have reported expression profiles of PG G/H synthases (PGHS-1 and PGHS-2), PGE synthase (mPGES-1), and PGF synthase (PGFS) in the endometrium of cyclic and pregnant pigs. In the present study, expression of above-mentioned PG synthesis enzymes and PG 9-ketoreductase (CBR1), which converts PGE(2) into PGF(2alpha), and the PGE(2)/PGF(2alpha) ratios were investigated in porcine peri- and post-implantation conceptuses. Furthermore, expression of CBR1 was examined in the endometrium. PGHS-2 and mPGES-1 were upregulated, and PGHS-1, PGFS, and CBR1 were downregulated in conceptuses during trophoblastic elongation. A second increase of mPGES-1 mRNA occurred after days 20-21 of pregnancy. After initiation of implantation, expression of PGHS-1, PGFS, and CBR1 in conceptuses increased and remained higher until days 24-25 of pregnancy. Comparison of the endometrial CBR1 protein expression in cyclic and pregnant gilts revealed upregulation on days 16-17 of the cycle and downregulation on days 10-11 of pregnancy. In conclusion, reciprocal expression of PGHS-2, mPGES-1, PGFS, and CBR1 in day 10-13 conceptuses and decrease of endometrial CBR1 may be important in increasing the PGE(2)/PGF(2alpha) ratio during maternal recognition of pregnancy. This study indicates that PGE(2) produced via PGHS-2 and mPGES-1 in conceptus may be involved in corpus luteum control. Moreover, high expression of conceptus PGHS-1, mPGES-1, PGFS, and CBR1 after initiation of implantation suggests their significant role in placentation.
[Mh] Termos MeSH primário: Oxirredutases do Álcool/análise
Endométrio/enzimologia
Hidroxiprostaglandina Desidrogenases/análise
Oxirredutases Intramoleculares/análise
Prenhez/metabolismo
Prostaglandina-Endoperóxido Sintases/análise
[Mh] Termos MeSH secundário: Oxirredutases do Álcool/genética
Aldeído Redutase
Aldo-Ceto Redutases
Animais
Western Blotting/métodos
Dinoprosta/análogos & derivados
Dinoprosta/análise
Dinoprostona/análise
Implantação do Embrião/fisiologia
Estruturas Embrionárias/química
Endométrio/química
Ciclo Estral
Feminino
Idade Gestacional
Hidroxiprostaglandina Desidrogenases/genética
Oxirredutases Intramoleculares/genética
Gravidez
Prostaglandina-E Sintases
Prostaglandina-Endoperóxido Sintases/genética
RNA Mensageiro/análise
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Suínos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Messenger); 27376-76-7 (15-keto-13,14-dihydroprostaglandin F2alpha); B7IN85G1HY (Dinoprost); EC 1.1.- (Alcohol Oxidoreductases); EC 1.1.1.- (Aldo-Keto Reductases); EC 1.1.1.- (Hydroxyprostaglandin Dehydrogenases); EC 1.1.1.188 (prostaglandin-F synthase); EC 1.1.1.21 (Aldehyde Reductase); EC 1.14.99.1 (Prostaglandin-Endoperoxide Synthases); EC 5.3.- (Intramolecular Oxidoreductases); EC 5.3.99.3 (Prostaglandin-E Synthases); K7Q1JQR04M (Dinoprostone)
[Em] Mês de entrada:0711
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:070901
[St] Status:MEDLINE


  8 / 199 MEDLINE  
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[PMID]:17761886
[Au] Autor:Fiúza UM; Arias AM
[Ad] Endereço:Department of Genetics, University of Cambridge, Cambridge CB2 3EH, UK. umf20@cam.ac.uk
[Ti] Título:Cell and molecular biology of Notch.
[So] Source:J Endocrinol;194(3):459-74, 2007 Sep.
[Is] ISSN:0022-0795
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Notch signalling is a cell-cell communication process, which allows the establishment of patterns of gene expression and differentiation, regulates binary cell fate choice and the maintenance of stem cell populations. So far, the data published has elucidated the main players in the Notch signalling pathway. However, its regulatory mechanisms are exhibiting an increasing complexity which could account for the multitude of roles it has during development and in adult organisms. In this review, we will describe the multiple roles of Notch and how various factors can regulate Notch signalling.
[Mh] Termos MeSH primário: Estruturas Embrionárias/metabolismo
Regulação da Expressão Gênica no Desenvolvimento
Receptores Notch/metabolismo
Transdução de Sinais/fisiologia
[Mh] Termos MeSH secundário: Animais
Proteínas de Ligação ao Cálcio/metabolismo
Diferenciação Celular/fisiologia
Drosophila/metabolismo
Proteínas de Drosophila
Sistema Endócrino/embriologia
Sistema Endócrino/metabolismo
Seres Humanos
Peptídeos e Proteínas de Sinalização Intercelular/metabolismo
Peptídeos e Proteínas de Sinalização Intracelular
Proteína Jagged-1
Proteínas de Membrana/metabolismo
Receptores Notch/genética
Proteínas Serrate-Jagged
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Nm] Nome de substância:
0 (Calcium-Binding Proteins); 0 (Drosophila Proteins); 0 (Intercellular Signaling Peptides and Proteins); 0 (Intracellular Signaling Peptides and Proteins); 0 (Jagged-1 Protein); 0 (Membrane Proteins); 0 (Receptors, Notch); 0 (Ser protein, Drosophila); 0 (Serrate-Jagged Proteins); 0 (delta protein)
[Em] Mês de entrada:0711
[Cu] Atualização por classe:161124
[Lr] Data última revisão:
161124
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:070901
[St] Status:MEDLINE


  9 / 199 MEDLINE  
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[PMID]:17726275
[Au] Autor:Gaillard F
[Ad] Endereço:Institute of Physiology and Cellular Biology, UMR 6187 CNRS, Faculty of Sciences, 40 avenue du Recteur Pineau, F-86022 Poitiers, France. Frederic.Gaillard@univ-poitiers.fr
[Ti] Título:Heterotopic, not homotopic, fetal occipital allografts in adult hosts project to visual-related extracortical targets.
[So] Source:Restor Neurol Neurosci;25(2):161-75, 2007.
[Is] ISSN:0922-6028
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:PURPOSE: Fetal occipital allografts implanted into the posterior cortex of adult mice project massively throughout the ipsilateral pallium of the host, but rarely outside this domain (Gaillard et al., 2004). The present study was undertaken to examine in detail whether this pattern is specific to graft location. METHODS: Cortical fragments corresponding to presumptive occipital areas were harvested from E15 mice fetuses expressing ubiquitously the eGFP protein, and implanted in correct (homotopic) and incorrect (heterotopic) cortical loci in wild-type adults. Two months later, efferents were detected by immunohistochemistry and quantified on selected DAB-treated sections. RESULTS: The present findings show (i) that robust projections are present in the ipsilateral host cortex regardless of the graft location; (ii) that 55% the grafts located in parietal and frontal cortices have obvious but sparse callosal and subcortical projections; and (iii) that grafts placed in occipital areas never contact ipsilateral subcortical targets, likely because graft-related axons are unable to cross obliquely the thalamocortical fascicles in the underlying white matter. CONCLUSIONS: These puzzling results question the use of transplantation strategies for repairing damaged networks in adults where rewiring involves complex white matter trajectories.
[Mh] Termos MeSH primário: Encéfalo/fisiologia
Transplante de Tecido Fetal
Lobo Occipital/embriologia
Transmissão Sináptica
Transplante Heterotópico
Vias Visuais/fisiologia
[Mh] Termos MeSH secundário: Animais
Biotina/análogos & derivados
Dextranos
Estruturas Embrionárias/fisiologia
Corantes Fluorescentes
Lobo Frontal/fisiologia
Proteínas de Fluorescência Verde
Imuno-Histoquímica
Técnicas In Vitro
Camundongos
Lobo Parietal/fisiologia
Transplante Homólogo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Dextrans); 0 (Fluorescent Dyes); 0 (biotinylated dextran amine); 0 (enhanced green fluorescent protein); 147336-22-9 (Green Fluorescent Proteins); 6SO6U10H04 (Biotin)
[Em] Mês de entrada:0711
[Cu] Atualização por classe:161124
[Lr] Data última revisão:
161124
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:070830
[St] Status:MEDLINE


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Fotocópia
[PMID]:17726269
[Au] Autor:Watanabe K; Nakamura M; Okano H; Toyama Y
[Ad] Endereço:Department of Orthopaedic Surgery, Keio University 35 Shinanomachi, Shinjuku, Tokyo 160-8582, Japan.
[Ti] Título:Establishment of three-dimensional culture of neural stem/progenitor cells in collagen Type-1 Gel.
[So] Source:Restor Neurol Neurosci;25(2):109-17, 2007.
[Is] ISSN:0922-6028
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:PURPOSE: Recent studies demonstrated that transplanting neural stem/precursor cells (NSPCs) into an injured spinal cord of adult rat promotes functional recovery. The functional recovery from spinal cord injury might be enhanced by transplanting NSPCs with a scaffold that fills the cavity, entraps the NSPCs in the cavity, and acts as an attachment for neurite extension. We recently focused on collagen type-1 as a scaffold for NSPC transplantation into the injured spinal cord. In the present study, we determined the optimal conditions for culturing NSPCs in 3D collagen type-1 gel with respect to cell survival and cell migration. We, then, evaluated the ability of NSPCs to differentiate under the optimal condition. METHODS: NSPCs were derived from the striatum of rat embryos. To determine the optimal cell density and collagen concentration for 3D collagen gel culture for NSPC, we performed viability assay and migration assay. Then, we examined the proportion of phenotypes differentiated from NSPC in that optimal condition. RESULTS: In viability assay, the viability rate increased as the NSPC density increased, and peaked at 1 x 10(7) to 5 x 10(7) cell/ml. For collagen concentration, the viability rate increased as the collagen concentration decreased. In migration assay, cell migration was most extensive at collagen concentrations between 0.3 and 0.75 mg/ml. Migration distances gradually declined as collagen concentrations increased. In the optimal condition, NSPCs differentiated into neurons (40.1%), astrocytes (53.1%), and oligodendrocytes (5.3%) in 3D collagen gel culture. CONCLUSION: The optimal conditions for NSPC culture in 3D collagen gel was a cell density between 1 x 10(7) and 5 x 10(7) cells/ml and a collagen concentration between 0.5 and 0.75 mg/ml. Under the condition, NSPCs could differentiate into neurons, astrocytes, and oligodendrocytes.
[Mh] Termos MeSH primário: Colágeno Tipo I
Técnicas Citológicas
Neurônios/citologia
Esferoides Celulares
Células-Tronco/citologia
[Mh] Termos MeSH secundário: Animais
Animais Geneticamente Modificados
Astrócitos/citologia
Contagem de Células
Diferenciação Celular
Movimento Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Colágeno Tipo I/administração & dosagem
Colágeno Tipo I/farmacologia
Relação Dose-Resposta a Droga
Estruturas Embrionárias
Géis
Proteínas de Fluorescência Verde/genética
Neurônios/fisiologia
Oligodendroglia/citologia
Concentração Osmolar
Ratos
Ratos Sprague-Dawley
Células-Tronco/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Collagen Type I); 0 (Gels); 0 (enhanced green fluorescent protein); 147336-22-9 (Green Fluorescent Proteins)
[Em] Mês de entrada:0711
[Cu] Atualização por classe:140729
[Lr] Data última revisão:
140729
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:070830
[St] Status:MEDLINE



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