Base de dados : MEDLINE
Pesquisa : A16.331.042 [Categoria DeCS]
Referências encontradas : 25 [refinar]
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  1 / 25 MEDLINE  
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[PMID]:27235683
[Au] Autor:Sakaguchi N; Maeda K
[Ad] Endereço:WPI Immunology Frontier Research Center (IFReC), Osaka University, Suita, Osaka, Japan; Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan. Electronic address: nobusaka@ifrec.osaka-u.ac.jp.
[Ti] Título:Germinal Center B-Cell-Associated Nuclear Protein (GANP) Involved in RNA Metabolism for B Cell Maturation.
[So] Source:Adv Immunol;131:135-86, 2016.
[Is] ISSN:1557-8445
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Germinal center B-cell-associated nuclear protein (GANP) is upregulated in germinal center B cells against T-cell-dependent antigens in mice and humans. In mice, GANP depletion in B cells impairs antibody affinity maturation. Conversely, its transgenic overexpression augments the generation of high-affinity antigen-specific B cells. GANP associates with AID in the cytoplasm, shepherds AID into the nucleus, and augments its access to the rearranged immunoglobulin (Ig) variable (V) region of the genome in B cells, thereby precipitating the somatic hypermutation of V region genes. GANP is also upregulated in human CD4(+) T cells and is associated with APOBEC3G (A3G). GANP interacts with A3G and escorts it to the virion cores to potentiate its antiretroviral activity by inactivating HIV-1 genomic cDNA. Thus, GANP is characterized as a cofactor associated with AID/APOBEC cytidine deaminase family molecules in generating diversity of the IgV region of the genome and genetic alterations of exogenously introduced viral targets. GANP, encoded by human chromosome 21, as well as its mouse equivalent on chromosome 10, contains a region homologous to Saccharomyces Sac3 that was characterized as a component of the transcription/export 2 (TREX-2) complex and was predicted to be involved in RNA export and metabolism in mammalian cells. The metabolism of RNA during its maturation, from the transcription site at the chromosome within the nucleus to the cytoplasmic translation apparatus, needs to be elaborated with regard to acquired and innate immunity. In this review, we summarize the current knowledge on GANP as a component of TREX-2 in mammalian cells.
[Mh] Termos MeSH primário: Acetiltransferases/metabolismo
Linfócitos B/fisiologia
Blastodisco/imunologia
Linfócitos T CD4-Positivos/fisiologia
Peptídeos e Proteínas de Sinalização Intracelular/metabolismo
RNA/metabolismo
[Mh] Termos MeSH secundário: Desaminase APOBEC-3G/metabolismo
Acetiltransferases/genética
Animais
Afinidade de Anticorpos
Formação de Anticorpos
Diferenciação Celular
Exodesoxirribonucleases/metabolismo
Seres Humanos
Peptídeos e Proteínas de Sinalização Intracelular/genética
Fosfoproteínas/metabolismo
RNA/genética
Hipermutação Somática de Imunoglobulina
Transcrição Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Intracellular Signaling Peptides and Proteins); 0 (Phosphoproteins); 63231-63-0 (RNA); EC 2.3.1.- (Acetyltransferases); EC 2.3.1.- (MCM3AP protein, human); EC 3.1.- (Exodeoxyribonucleases); EC 3.1.16.- (TREX2 protein, human); EC 3.5.4.5 (APOBEC-3G Deaminase)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170803
[Lr] Data última revisão:
170803
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160529
[St] Status:MEDLINE


  2 / 25 MEDLINE  
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[PMID]:26957633
[Au] Autor:Bakst MR; Welch GR; Camp MJ
[Ad] Endereço:Animal Biosciences and Biotechnology Laboratory murray.bakst@ars.usda.gov.
[Ti] Título:Observations of turkey eggs stored up to 27 days and incubated for 8 days: embryo developmental stage and weight differences and the differentiation of fertilized from unfertilized germinal discs.
[So] Source:Poult Sci;95(5):1165-72, 2016 May.
[Is] ISSN:0032-5791
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:For logistical reasons, egg storage prior to incubation is a growing practice in the commercial turkey industry. Yet the consequence of increasing egg storage over 7 d is a progressive increase in embryo mortality. The objective of this study was to provide the information necessary to differentiate an early dead embryo from an unfertilized egg after 8 days of incubation (DOI). Five groups of eggs each from inseminated and virgin hens were stored for progressively increasing periods of time (5-d or less, 6 to 10 d, 11 to 15 d, 16 to 20 d, and 21 to 27 d) and incubated. At 8 DOI, eggs were examined and the stage of development (Hamburger and Hamilton, 1951) and embryo weights in normally developed eggs were determined. There was a significant negative correlation between the stage of development and embryo weight with increasing storage periods. All remaining eggs from the inseminated and virgin hens were broken-out and the appearance of the yolk and the fertilized and unfertilized germinal discs examined. The yolks of both hen groups with unfertilized ova maintained a homogeneous uniform yellow-orange color. In contrast, yolks of ova that had been fertilized, with or without early-dead embryos, and yolks from virgin hens that showed evidence of parthenogenetic development (3%) had a heterogeneous appearance. Using fluorescence microscopy, the heterogeneous appearance was due to sheets of aberrant cells and less frequently dispersed cells and folds of the perivitelline layer. It was concluded that clear egg breakouts need to be performed to more accurately assess the impact of egg storage on embryonic mortality. Furthermore, such breakouts should be performed with a high intensity light directed across the surface of the germinal disc to clearly differentiate the subtle differences between an early-dead embryo and an unfertilized germinal disc.
[Mh] Termos MeSH primário: Criação de Animais Domésticos/métodos
Blastodisco/fisiologia
Embrião não Mamífero/fisiologia
Perus/embriologia
[Mh] Termos MeSH secundário: Animais
Partenogênese
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1606
[Cu] Atualização por classe:160409
[Lr] Data última revisão:
160409
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160310
[St] Status:MEDLINE
[do] DOI:10.3382/ps/pew010


  3 / 25 MEDLINE  
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[PMID]:25972012
[Au] Autor:Aslam MA; Schokker D; Groothuis TG; de Wit AA; Smits MA; Woelders H
[Ad] Endereço:Animal Breeding and Genomics Centre, Wageningen UR Livestock Research, Lelystad, The Netherlands.
[Ti] Título:Association of Egg Mass and Egg Sex: Gene Expression Analysis from Maternal RNA in the Germinal Disc Region of Layer Hens (Gallus gallus).
[So] Source:Biol Reprod;92(6):157, 2015 Jun.
[Is] ISSN:1529-7268
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Female birds have been shown to manipulate offspring sex ratio. However, mechanisms of sex ratio bias are not well understood. Reduced feed availability and change in body condition can affect the mass of eggs in birds that could lead to a skew in sex ratio. We employed feed restriction in laying chickens (Gallus gallus) to induce a decrease in body condition and egg mass using 45 chicken hens in treatment and control groups. Feed restriction led to an overall decline of egg mass. In the second period of treatment (Days 9-18) with more severe feed restriction and a steeper decline of egg mass, the sex ratio per hen (proportion of male eggs) had a significant negative association with mean egg mass per hen. Based on this association, two groups of hens were selected from feed restriction group, that is, hens producing male bias with low egg mass and hens producing female bias with high egg mass with overall sex ratios of 0.71 and 0.44 respectively. Genomewide transcriptome analysis on the germinal disks of F1 preovulatory follicles collected at the time of occurrence of meiosis-I was performed. We did not find significantly differentially expressed genes in these two groups of hens. However, gene set enrichment analysis showed that a number of cellular processes related to cell cycle progression, mitotic/meiotic apparatus, and chromosomal movement were enriched in female-biased hens or high mean egg mass as compared with male-biased hens or low mean egg mass. The differentially expressed gene sets may be involved in meiotic drive regulating sex ratio in the chicken.
[Mh] Termos MeSH primário: Fenômenos Fisiológicos da Nutrição Animal
Blastodisco/metabolismo
Peso Corporal/fisiologia
Privação de Alimentos/fisiologia
Óvulo/metabolismo
[Mh] Termos MeSH secundário: Animais
Galinhas
Feminino
Razão de Masculinidade
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1603
[Cu] Atualização por classe:150626
[Lr] Data última revisão:
150626
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150515
[St] Status:MEDLINE
[do] DOI:10.1095/biolreprod.114.123380


  4 / 25 MEDLINE  
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[PMID]:25253730
[Au] Autor:Nishio S; Kohno Y; Iwata Y; Arai M; Okumura H; Oshima K; Nadano D; Matsuda T
[Ad] Endereço:Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya, Japan.
[Ti] Título:Glycosylated chicken ZP2 accumulates in the egg coat of immature oocytes and remains localized to the germinal disc region of mature eggs.
[So] Source:Biol Reprod;91(5):107, 2014 Nov.
[Is] ISSN:1529-7268
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Vertebrate eggs are surrounded by an egg coat, which is a specific extracellular egg matrix consisting of several glycoproteins with a conserved zona pellucida (ZP) domain. Two mammalian egg coat subunits, ZP2 and ZP3, have been suggested to act as sperm receptors. In bird eggs, however, ZP2 has never been identified in the egg coat of mature oocytes and ovulated eggs. Here we report that chicken ZP2 is expressed in immature small follicles and remains as an egg-coat component locally in the germinal disc region of mature eggs. RT-PCR analysis indicated marked expression of the ZP2 and ZP4 genes in the granulosa cells of immature white follicles, whereas the ZP3 and ZPD genes showed marked expression in the cells of maturing yellow follicles. ZP2 was identified in the egg coat isolated from immature follicles as a heavily N-glycosylated glycoprotein of ∼200 kDa, which was enzymatically converted to a 70-kDa deglycosylated form. Immunoblotting and immunohistological analyses showed that ZP2 was localized around the germinal disc region of mature follicles. ZP2 was accumulated in the egg coat of immature white follicles at the earlier stages of oocyte development and became a minor component in the egg coat of maturing yellow follicles, except for the germinal disc region. Localization of ZP2 in the germinal disc region of mature eggs, where sperm bind to the egg coat at high density, suggests some role for ZP2 in the preferential binding and penetration of sperm in the germinal disc region of bird eggs.
[Mh] Termos MeSH primário: Blastodisco/metabolismo
Membrana Celular/metabolismo
Galinhas
Proteínas do Ovo/metabolismo
Glicoproteínas de Membrana/metabolismo
Oócitos/metabolismo
Óvulo/metabolismo
Receptores de Superfície Celular/metabolismo
[Mh] Termos MeSH secundário: Animais
Galinhas/genética
Galinhas/metabolismo
Proteínas do Ovo/genética
Casca de Ovo/metabolismo
Feminino
Expressão Gênica
Glicosilação
Glicoproteínas de Membrana/genética
Oogênese/fisiologia
Receptores de Superfície Celular/genética
Interações Espermatozoide-Óvulo/genética
Distribuição Tecidual
Glicoproteínas da Zona Pelúcida
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Egg Proteins); 0 (Membrane Glycoproteins); 0 (Receptors, Cell Surface); 0 (Zona Pellucida Glycoproteins)
[Em] Mês de entrada:1508
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140926
[St] Status:MEDLINE
[do] DOI:10.1095/biolreprod.114.119826


  5 / 25 MEDLINE  
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[PMID]:23912483
[Au] Autor:Wang MY; Guo QH; Du XZ; Zhou L; Luo Q; Zeng QH; Wang JL; Zhao HB; Wang YF
[Ad] Endereço:Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Science, Central China Normal University, Wuhan, 430079, People's Republic of China.
[Ti] Título:HIRA is essential for the development of gibel carp.
[So] Source:Fish Physiol Biochem;40(1):235-44, 2014 Feb.
[Is] ISSN:1573-5168
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:HIRA is one of the chaperones of histone H3.3. Mutation of Hira results in embryonic lethality in mice, suggesting a critical role in embryogenesis. However, Hira-mutated Drosophila may survive to adults, indicating that it is dispensable in Drosophila development. The role of Hira in fish development is unknown. In this study we first investigated the expression of Hira during embryogenesis of gibel carp (Carassius auratus gibelio) by whole-mount in situ hybridization. We found that Hira signal appeared ubiquitously in the early embryos. After gastrulation, it appeared mainly along the anterior-posterior axis, including the tail bud. In hatching period, the signal was detected in head, heart, and the endoderm region on the back of yolk. Then by microinjection with morpholino-HIRA at the beginning of development, we observed delayed gastrulation and abnormal somitogenesis in gibel carp embryos. The HIRA morphants exhibited short trunk, limited yolk extension, and twisted tail. Most of the mutants died during embryogenesis or shortly after hatching. The rest of the HIRA morphants could survive to larvae but with severe defects in organogenesis. These data suggest that HIRA may be essential for the development of gibel carp, and this function is conserved in vertebrates.
[Mh] Termos MeSH primário: Desenvolvimento Embrionário
Carpa Dourada/embriologia
Chaperonas de Histonas/fisiologia
[Mh] Termos MeSH secundário: Animais
Blastodisco/metabolismo
Carpas/genética
Feminino
Carpa Dourada/genética
Hibridização In Situ
Masculino
Mutação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Histone Chaperones)
[Em] Mês de entrada:1410
[Cu] Atualização por classe:171020
[Lr] Data última revisão:
171020
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130806
[St] Status:MEDLINE
[do] DOI:10.1007/s10695-013-9839-x


  6 / 25 MEDLINE  
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[PMID]:23452600
[Au] Autor:Maître JL; Berthoumieux H; Gabriel Krens SF; Salbreux G; Jülicher F; Paluch E; Heisenberg CP
[Ti] Título:[Cell adhesion mechanics of zebrafish gastrulation].
[Ti] Título:La mécanique de l'adhésion cellulaire dans la gastrulation du poisson zèbre..
[So] Source:Med Sci (Paris);29(2):147-50, 2013 Feb.
[Is] ISSN:0767-0974
[Cp] País de publicação:France
[La] Idioma:fre
[Mh] Termos MeSH primário: Gastrulação/fisiologia
Peixe-Zebra/embriologia
[Mh] Termos MeSH secundário: Animais
Fenômenos Biomecânicos/fisiologia
Blastodisco/citologia
Blastodisco/embriologia
Adesão Celular/fisiologia
Comunicação Celular/fisiologia
Embrião não Mamífero
Modelos Biológicos
[Pt] Tipo de publicação:NEWS
[Em] Mês de entrada:1306
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130305
[St] Status:MEDLINE
[do] DOI:10.1051/medsci/2013292011


  7 / 25 MEDLINE  
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[PMID]:23063361
[Au] Autor:Jory A; Estella C; Giorgianni MW; Slattery M; Laverty TR; Rubin GM; Mann RS
[Ad] Endereço:Department of Biochemistry and Molecular Biophysics, Columbia University Medical Center, 701 W. 168th Street, HHSC 1104, New York, NY 10032, USA.
[Ti] Título:A survey of 6,300 genomic fragments for cis-regulatory activity in the imaginal discs of Drosophila melanogaster.
[So] Source:Cell Rep;2(4):1014-24, 2012 Oct 25.
[Is] ISSN:2211-1247
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Over 6,000 fragments from the genome of Drosophila melanogaster were analyzed for their ability to drive expression of GAL4 reporter genes in the third-instar larval imaginal discs. About 1,200 reporter genes drove expression in the eye, antenna, leg, wing, haltere, or genital imaginal discs. The patterns ranged from large regions to individual cells. About 75% of the active fragments drove expression in multiple discs; 20% were expressed in ventral, but not dorsal, discs (legs, genital, and antenna), whereas ∼23% were expressed in dorsal but not ventral discs (wing, haltere, and eye). Several patterns, for example, within the leg chordotonal organ, appeared a surprisingly large number of times. Unbiased searches for DNA sequence motifs suggest candidate transcription factors that may regulate enhancers with shared activities. Together, these expression patterns provide a valuable resource to the community and offer a broad overview of how transcriptional regulatory information is distributed in the Drosophila genome.
[Mh] Termos MeSH primário: Proteínas de Drosophila/metabolismo
Drosophila melanogaster/metabolismo
Genoma
Discos Imaginais/metabolismo
Fatores de Transcrição/metabolismo
[Mh] Termos MeSH secundário: Animais
Animais Geneticamente Modificados
Antenas de Artrópodes/metabolismo
Blastodisco/metabolismo
Bases de Dados Factuais
Proteínas de Drosophila/genética
Drosophila melanogaster/genética
Olho/metabolismo
Genes Reporter
Elementos Reguladores de Transcrição
Fatores de Transcrição/genética
Asas de Animais/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Drosophila Proteins); 0 (GAL4 protein, Drosophila); 0 (Transcription Factors)
[Em] Mês de entrada:1303
[Cu] Atualização por classe:161019
[Lr] Data última revisão:
161019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:121016
[St] Status:MEDLINE


  8 / 25 MEDLINE  
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[PMID]:22627133
[Au] Autor:Yoshitome S; Furuno N; Prigent C; Hashimoto E
[Ad] Endereço:Faculty of Pharmacy, Iwaki Meisei University, Iwaki 970-8551, Japan. yoshitom@iwakimu.ac.jp
[Ti] Título:The subcellular localization of cyclin B2 is required for bipolar spindle formation during Xenopus oocyte maturation.
[So] Source:Biochem Biophys Res Commun;422(4):770-5, 2012 Jun 15.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cyclins B1 and B2 are subtypes of cyclin B, a regulatory subunit of a maturation/M-phase promoting factor, and they are also highly conserved in many vertebrate species. Cyclin B1 is essential for mitosis, whereas cyclin B2 is regarded as dispensable. However, the overexpression of the cyclin B2 N-terminus containing the cytoplasmic retention signal, but not cyclin B1, inhibits bipolar spindle formation in Xenopus oocytes and embryos. Here we show that endogenous cyclin B2 was localized in and around the germinal vesicle. The perinuclear localization of cyclin B2 was perturbed by the overexpression of its N-terminus containing the cytoplasmic retention signal, which resulted in a spindle defect. This spindle defect was rescued by the overexpression of bipolar kinesin Eg5, which is located at the perinuclear region in the proximity of endogenous cyclin B2. These results demonstrate that the proper localization of cyclin B2 is essential for bipolar spindle formation in Xenopus oocytes.
[Mh] Termos MeSH primário: Blastodisco/metabolismo
Ciclina B2/metabolismo
Oócitos/crescimento & desenvolvimento
Fuso Acromático/metabolismo
Proteínas de Xenopus/metabolismo
Xenopus laevis/embriologia
[Mh] Termos MeSH secundário: Animais
Feminino
Meiose
Oócitos/citologia
Oócitos/metabolismo
Xenopus laevis/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cyclin B2); 0 (Xenopus Proteins); 0 (ccnb2 protein, Xenopus)
[Em] Mês de entrada:1209
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120526
[St] Status:MEDLINE
[do] DOI:10.1016/j.bbrc.2012.05.080


  9 / 25 MEDLINE  
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[PMID]:22336033
[Au] Autor:Aslam MA; Hulst M; Hoving-Bolink RA; de Wit AA; Smits MA; Woelders H
[Ad] Endereço:Animal Breeding and Genomics Centre, Wageningen UR Livestock Research, PO Box 65, 8200 AB Lelystad, The Netherlands.
[Ti] Título:A reliable method for sexing unincubated bird eggs for studying primary sex ratio.
[So] Source:Mol Ecol Resour;12(3):421-7, 2012 May.
[Is] ISSN:1755-0998
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In birds, offspring sex ratio manipulation by mothers is now well established with potentially important consequences for evolution and animal breeding. In most studies on primary sex ratio of birds, eggs are sexed after incubation by the use of PCR methods targeted to the sex-linked CHD1 genes. Sexing of unincubated eggs would be preferred, but as fertile and infertile blastodiscs cannot be distinguished macroscopically, errors could arise from PCR amplifications of parental DNA associated with the vitelline membrane of infertile eggs. In this study, we stained blastodiscs without the vitelline membrane with Hoechst 33342. This allowed unequivocal distinction between fertile and infertile blastodiscs. Fertile blastodiscs contained thousands of fluorescent nuclei, whereas no nuclei were seen in infertile eggs. In addition, after nucleic acid analysis, fertile blastodiscs yielded much stronger chromosomal DNA and CHD1-targeted PCR bands on agarose gels compared with infertile blastodiscs. These findings indicate that fertile blastodiscs contain much more embryonic DNA than parental DNA, allowing reliable sexing of the fertile eggs. The differences between fertile and infertile blastodiscs in chromosomal DNA and CHD1 PCR banding intensities alone could also be used to distinguish fertile from infertile eggs without using Hoechst staining. We conclude that identifying fertile blastodiscs either by Hoechst staining or by analyzing the yield of chromosomal DNA and CHD1-PCR products, combined with CHD1-targeted PCR amplification, presents an easy and reliable method to sex unincubated eggs.
[Mh] Termos MeSH primário: Aves/embriologia
Blastodisco/metabolismo
Análise para Determinação do Sexo/métodos
Razão de Masculinidade
Coloração e Rotulagem/métodos
[Mh] Termos MeSH secundário: Animais
Proteínas Aviárias/genética
Benzimidazóis/metabolismo
Proteínas de Ligação a DNA/genética
Corantes Fluorescentes/metabolismo
Reação em Cadeia da Polimerase/métodos
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Avian Proteins); 0 (Benzimidazoles); 0 (CHD-W protein, Gallus gallus); 0 (DNA-Binding Proteins); 0 (Fluorescent Dyes); P976261J69 (bisbenzimide ethoxide trihydrochloride)
[Em] Mês de entrada:1207
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120217
[St] Status:MEDLINE
[do] DOI:10.1111/j.1755-0998.2012.03120.x


  10 / 25 MEDLINE  
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[PMID]:22213374
[Au] Autor:Mohanty BK; Gupta BL
[Ad] Endereço:Department of Zoology, Cambridge University, Cambridge, UK. basant_colorado_edu@yahoo.com
[Ti] Título:A marked animal-vegetal polarity in the localization of Na(+),K(+) -ATPase activity and its down-regulation following progesterone-induced maturation.
[So] Source:Mol Reprod Dev;79(2):138-60, 2012 Feb.
[Is] ISSN:1098-2795
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The stage-VI Xenopus oocyte has a very distinct animal-vegetal polarity with structural and functional asymmetry. In this study, we show the expression and distribution pattern of Na(+),K(+) -ATPase in stage-VI oocytes, and its changes following progesterone-induced maturation. Using enzyme-specific electron microscopy phosphatase histochemistry, [(3) H]-ouabain autoradiography, and immunofluorescence cytochemistry at light microscopic level, we find that Na(+),K(+) -ATPase activity is mainly confined to the animal hemisphere. Electron microscopy histochemical results also suggest that polarized distribution of Na(+),K(+) -ATPase activity persists following progesterone-induced maturation, and it becomes gradually more polarized towards the animal pole. The time course following progesterone-induced maturation suggests that there is an initial up-regulation and then gradual down-regulation of Na(+),K(+) -ATPase activity leading to germinal vesicle breakdown (GVBD). By GVBD, the Na(+),K(+) -ATPase activity is completely down-regulated due to endocytotic removal of pump molecules from the plasma membrane into the sub-cortical region of the oocyte. This study provides the first direct evidence for a marked asymmetric localization of Na(+),K(+) -ATPase activity in any vertebrate oocyte. Here, we propose that such asymmetry in Na(+),K(+) -ATPase activity in stage-VI oocytes, and their down-regulation following progesterone-induced maturation, is likely to have a role in the active state of the germinal vesicle in stage-VI oocytes and chromosomal condensation after GVBD.
[Mh] Termos MeSH primário: Polaridade Celular
Oócitos/citologia
Oogênese/efeitos dos fármacos
Progesterona/farmacologia
ATPase Trocadora de Sódio-Potássio/metabolismo
[Mh] Termos MeSH secundário: Animais
Blastodisco/metabolismo
Blastodisco/ultraestrutura
Núcleo Celular/metabolismo
Núcleo Celular/ultraestrutura
Polaridade Celular/efeitos dos fármacos
Fase de Clivagem do Zigoto/citologia
Fase de Clivagem do Zigoto/efeitos dos fármacos
Fase de Clivagem do Zigoto/metabolismo
Fase de Clivagem do Zigoto/fisiologia
Regulação para Baixo/efeitos dos fármacos
Feminino
Microscopia Eletrônica de Transmissão
Modelos Biológicos
Oócitos/efeitos dos fármacos
Oócitos/metabolismo
Oócitos/ultraestrutura
Receptores de Progesterona/metabolismo
Distribuição Tecidual
Xenopus laevis
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Receptors, Progesterone); 4G7DS2Q64Y (Progesterone); EC 3.6.3.9 (Sodium-Potassium-Exchanging ATPase)
[Em] Mês de entrada:1205
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120104
[St] Status:MEDLINE
[do] DOI:10.1002/mrd.22012



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