Base de dados : MEDLINE
Pesquisa : A16.441 [Categoria DeCS]
Referências encontradas : 3252 [refinar]
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  1 / 3252 MEDLINE  
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[PMID]:29033362
[Au] Autor:Barone V; Lang M; Krens SFG; Pradhan SJ; Shamipour S; Sako K; Sikora M; Guet CC; Heisenberg CP
[Ad] Endereço:Institute of Science and Technology Austria, 3400 Klosterneuburg, Austria.
[Ti] Título:An Effective Feedback Loop between Cell-Cell Contact Duration and Morphogen Signaling Determines Cell Fate.
[So] Source:Dev Cell;43(2):198-211.e12, 2017 Oct 23.
[Is] ISSN:1878-1551
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cell-cell contact formation constitutes an essential step in evolution, leading to the differentiation of specialized cell types. However, remarkably little is known about whether and how the interplay between contact formation and fate specification affects development. Here, we identify a positive feedback loop between cell-cell contact duration, morphogen signaling, and mesendoderm cell-fate specification during zebrafish gastrulation. We show that long-lasting cell-cell contacts enhance the competence of prechordal plate (ppl) progenitor cells to respond to Nodal signaling, required for ppl cell-fate specification. We further show that Nodal signaling promotes ppl cell-cell contact duration, generating a positive feedback loop between ppl cell-cell contact duration and cell-fate specification. Finally, by combining mathematical modeling and experimentation, we show that this feedback determines whether anterior axial mesendoderm cells become ppl or, instead, turn into endoderm. Thus, the interdependent activities of cell-cell signaling and contact formation control fate diversification within the developing embryo.
[Mh] Termos MeSH primário: Comunicação Celular
Linhagem da Célula
Retroalimentação Fisiológica
Gástrula/metabolismo
Morfogênese/fisiologia
Proteínas de Peixe-Zebra/metabolismo
Peixe-Zebra/metabolismo
[Mh] Termos MeSH secundário: Animais
Padronização Corporal
Diferenciação Celular
Embrião não Mamífero/citologia
Embrião não Mamífero/metabolismo
Desenvolvimento Embrionário
Gástrula/crescimento & desenvolvimento
Gastrulação/fisiologia
Regulação da Expressão Gênica no Desenvolvimento
Modelos Teóricos
Proteína Nodal/genética
Proteína Nodal/metabolismo
Transdução de Sinais
Células-Tronco/citologia
Células-Tronco/metabolismo
Peixe-Zebra/embriologia
Proteínas de Peixe-Zebra/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Nodal Protein); 0 (Zebrafish Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171017
[St] Status:MEDLINE


  2 / 3252 MEDLINE  
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[PMID]:28935526
[Au] Autor:Akahoshi T; Hotta K; Oka K
[Ad] Endereço:Department of Bioscience and Informatics, Faculty of Science and Technology, Keio University, Yokohama 223-8522, Japan.
[Ti] Título:Characterization of calcium transients during early embryogenesis in ascidians Ciona robusta (Ciona intestinalis type A) and Ciona savignyi.
[So] Source:Dev Biol;431(2):205-214, 2017 11 15.
[Is] ISSN:1095-564X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The calcium ion (Ca ) is an important second messenger, and a rapid increase in Ca level (Ca transient) is involved in various aspects of embryogenesis. Although Ca transients play an important role in early developmental stages, little is known about their dynamics throughout embryogenesis. Here, Ca transients were characterized by visualizing Ca dynamics in developing chordate embryos using a fluorescent protein-based Ca indicator, GCaMP6s in combination with finely tuned microscopy. Ca transients were detected in precursors of muscle cells in the late gastrula stage. In the neurula stage, repetitive Ca transients were observed in left and right neurogenic cells, including visceral ganglion (VG) precursors, and the duration of Ca transients was 39±4s. In the early tailbud stage, Ca transients were observed in differentiating precursors of nerve cord neurons. A small population of VG precursors showed rhythmical Ca transients with a duration of 22±4s, suggesting a central pattern generator (CPG) origin. At the mid tailbud stage, Ca transients were observed in a wide area of epidermal cells and named CTECs. The number and frequency of CTECs increased drastically in late tailbud stages, and the timing of the increase coincided with that of the relaxation of the tail bending. The experiment using Ca chelator showed that the CTECs were largely depending on the extracellular Ca . The waveform analysis of Ca transients revealed different features according to duration and frequency. The comprehensive characterization of Ca transients during early ascidian embryogenesis will help our understanding of the role of Ca signaling in chordate embryogenesis.
[Mh] Termos MeSH primário: Sinalização do Cálcio
Embrião não Mamífero/metabolismo
Desenvolvimento Embrionário
Urocordados/embriologia
Urocordados/metabolismo
[Mh] Termos MeSH secundário: Animais
Ciona intestinalis/embriologia
Ciona intestinalis/metabolismo
Gástrula/embriologia
Gástrula/metabolismo
Cauda/embriologia
Imagem com Lapso de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171111
[Lr] Data última revisão:
171111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170923
[St] Status:MEDLINE


  3 / 3252 MEDLINE  
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[PMID]:28668387
[Au] Autor:Li YL; Shao M; Shi DL
[Ad] Endereço:School of Life Sciences, Shandong University, 27, Shanda Nan Road, Jinan 250100, China.
[Ti] Título:Rac1 signalling coordinates epiboly movement by differential regulation of actin cytoskeleton in zebrafish.
[So] Source:Biochem Biophys Res Commun;490(3):1059-1065, 2017 Aug 26.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Dynamic cytoskeleton organization is essential for polarized cell behaviours in a wide variety of morphogenetic events. In zebrafish, epiboly involves coordinated cell shape changes and expansion of cell layers to close the blastopore, but many important regulatory aspects are still unclear. Especially, the spatio-temporal regulation and function of actin structures remain to be determined for a better understanding of the mechanisms that coordinate epiboly movement. Here we show that Rac1 signalling, likely functions downstream of phosphatiditylinositol-3 kinase, is required for F-actin organization during epiboly progression in zebtafish. Using a dominant negative mutant of Rac1 and specific inhibitors to block the activation of this pathway, we find that marginal contractile actin ring is sensitive to inhibition of Rac1 signalling. In particular, we identify a novel function for this actin structure in retaining the external yolk syncytial nuclei within the margin of enveloping layer for coordinated movement toward the vegetal pole. Furthermore, we find that F-actin bundles, progressively formed in the vegetal cortex of the yolk cell, act in concert with marginal actin ring and play an active role in pulling external yolk syncytial nuclei toward the vegetal pole direction. This study uncovers novel roles of different actin structures in orchestrating epiboly movement. It helps to provide insight into the mechanisms regulating cellular polarization during early development.
[Mh] Termos MeSH primário: Citoesqueleto de Actina/metabolismo
Transdução de Sinais
Proteínas de Peixe-Zebra/metabolismo
Peixe-Zebra/embriologia
Peixe-Zebra/metabolismo
Proteínas rac1 de Ligação ao GTP/metabolismo
[Mh] Termos MeSH secundário: Citoesqueleto de Actina/ultraestrutura
Animais
Forma Celular
Embrião não Mamífero/citologia
Embrião não Mamífero/embriologia
Embrião não Mamífero/metabolismo
Embrião não Mamífero/ultraestrutura
Gástrula/citologia
Gástrula/embriologia
Gástrula/metabolismo
Gástrula/ultraestrutura
Gastrulação
Fosfatidilinositol 3-Quinases/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Zebrafish Proteins); EC 2.7.1.- (Phosphatidylinositol 3-Kinases); EC 3.6.5.2 (rac1 GTP-Binding Protein)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170808
[Lr] Data última revisão:
170808
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170703
[St] Status:MEDLINE


  4 / 3252 MEDLINE  
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[PMID]:28623229
[Au] Autor:Dong X; Li J; He L; Gu C; Jia W; Yue Y; Li J; Zhang Q; Chu L; Zhao Q
[Ad] Endereço:From the Ministry of Education Key Laboratory of Model Animal for Disease Study, Model Animal Research Center, Nanjing University, Nanjing 210061, China.
[Ti] Título:Zebrafish Znfl1 proteins control the expression of gene in the posterior neuroectoderm by acting upstream of and genes.
[So] Source:J Biol Chem;292(31):13045-13055, 2017 Aug 04.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Transcription factors play crucial roles in patterning posterior neuroectoderm. Previously, zinc finger transcription factor was reported to be expressed in the posterior neuroectoderm of zebrafish embryos. However, its roles remain unknown. Here, we report that there are 13 copies of in the zebrafish genome, and all the paralogues share highly identical protein sequences and cDNA sequences. When are knocked down using a morpholino to inhibit their translation or dCas9-Eve to inhibit their transcription, the zebrafish gastrula displays reduced expression of , the marker gene for the posterior neuroectoderm. Further analyses reveal that diminishing produces the decreased expressions of , whereas overexpression of effectively rescues the reduced expression of in the posterior neuroectoderm. Additionally, knocking down causes the reduced expression of , a direct regulator of , in the posterior neuroectoderm, and overexpression of rescues the expression of in the knockdown embryos. In contrast, knocking down either or does not affect the expressions of Taken together, our results demonstrate that zebrafish control the expression of in the posterior neuroectoderm by acting upstream of and .
[Mh] Termos MeSH primário: Regulação da Expressão Gênica no Desenvolvimento
Proteínas de Homeodomínio/metabolismo
Proteínas do Tecido Nervoso/metabolismo
Placa Neural/metabolismo
Fator 3 de Transcrição de Octâmero/metabolismo
Fatores de Transcrição/metabolismo
Proteínas de Peixe-Zebra/metabolismo
[Mh] Termos MeSH secundário: Animais
Biomarcadores/metabolismo
Biologia Computacional
Gástrula/efeitos dos fármacos
Gástrula/metabolismo
Dosagem de Genes
Regulação da Expressão Gênica no Desenvolvimento/efeitos dos fármacos
Proteínas de Homeodomínio/genética
Hibridização In Situ
Microinjeções
Morfolinos/farmacologia
Proteínas do Tecido Nervoso/antagonistas & inibidores
Proteínas do Tecido Nervoso/genética
Placa Neural/efeitos dos fármacos
Placa Neural/embriologia
Neurogênese/efeitos dos fármacos
Fator 3 de Transcrição de Octâmero/antagonistas & inibidores
Fator 3 de Transcrição de Octâmero/genética
Interferência de RNA
RNA Antissenso/farmacologia
RNA Mensageiro/antagonistas & inibidores
RNA Mensageiro/metabolismo
Fatores de Transcrição/química
Fatores de Transcrição/genética
Peixe-Zebra
Proteínas de Peixe-Zebra/antagonistas & inibidores
Proteínas de Peixe-Zebra/química
Proteínas de Peixe-Zebra/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (HOXB1 homeodomain protein); 0 (Homeodomain Proteins); 0 (Morpholinos); 0 (Nerve Tissue Proteins); 0 (Octamer Transcription Factor-3); 0 (RNA, Antisense); 0 (RNA, Messenger); 0 (Sall4 protein, zebrafish); 0 (Transcription Factors); 0 (Zebrafish Proteins); 0 (Znfl1 protein, zebrafish); 0 (pou5f1 protein, zebrafish)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170825
[Lr] Data última revisão:
170825
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170618
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M117.777094


  5 / 3252 MEDLINE  
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[PMID]:28544452
[Au] Autor:Erkenbrack EM; Petsios E
[Ad] Endereço:Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California.
[Ti] Título:A Conserved Role for VEGF Signaling in Specification of Homologous Mesenchymal Cell Types Positioned at Spatially Distinct Developmental Addresses in Early Development of Sea Urchins.
[So] Source:J Exp Zool B Mol Dev Evol;328(5):423-432, 2017 Jul.
[Is] ISSN:1552-5015
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Comparative studies of early development in echinoderms are revealing the tempo and mode of alterations to developmental gene regulatory networks and to the cell types they specify. In euechinoid sea urchins, skeletogenic mesenchyme (SM) ingresses prior to gastrulation at the vegetal pole and aligns into a ring-like array with two bilateral pockets of cells, the sites where spiculogenesis will later occur. In cidaroid sea urchins, the anciently diverged sister clade to euechinoid sea urchins, a homologous SM cell type ingresses later in development, after gastrulation has commenced, and consequently at a distinct developmental address. Thus, a heterochronic shift of ingression of the SM cell type occurred in one of the echinoid lineages. In euechinoids, specification and migration of SM are facilitated by vascular endothelial growth factor (VEGF) signaling. We describe spatiotemporal expression of vegf and vegfr and experimental manipulations targeting VEGF signaling in the cidaroid Eucidaris tribuloides. Spatially, vegf and vegfr mRNA localizes similarly as in euechinoids, suggesting conserved deployment in echinoids despite their spatially distinct development addresses of ingression. Inhibition of VEGF signaling in E. tribuloides suggests its role in SM specification is conserved in echinoids. Temporal discrepancies between the onset of vegf expression and SM ingression likely result in previous observations of SM "random wandering" behavior. Our results indicate that, although the SM cell type in echinoids ingresses into distinct developmental landscapes, it retains a signaling mechanism that restricts their spatial localization to a conserved developmental address where spiculogenesis later occurs.
[Mh] Termos MeSH primário: Células Mesenquimais Estromais/classificação
Strongylocentrotus/metabolismo
Fator A de Crescimento do Endotélio Vascular/metabolismo
[Mh] Termos MeSH secundário: Animais
Evolução Biológica
Gástrula/metabolismo
Regulação da Expressão Gênica no Desenvolvimento
Larva/metabolismo
Células Mesenquimais Estromais/metabolismo
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Transdução de Sinais/fisiologia
Strongylocentrotus/genética
Fatores de Tempo
Fator A de Crescimento do Endotélio Vascular/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Messenger); 0 (Vascular Endothelial Growth Factor A)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170526
[St] Status:MEDLINE
[do] DOI:10.1002/jez.b.22743


  6 / 3252 MEDLINE  
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[PMID]:28504700
[Au] Autor:Khoueiry R; Sohni A; Thienpont B; Luo X; Velde JV; Bartoccetti M; Boeckx B; Zwijsen A; Rao A; Lambrechts D; Koh KP
[Ad] Endereço:KU Leuven Department of Development and Regeneration, Stem Cell Institute Leuven, Leuven, Belgium.
[Ti] Título:Lineage-specific functions of TET1 in the postimplantation mouse embryo.
[So] Source:Nat Genet;49(7):1061-1072, 2017 Jul.
[Is] ISSN:1546-1718
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The mammalian TET enzymes catalyze DNA demethylation. While they have been intensely studied as major epigenetic regulators, little is known about their physiological roles and the extent of functional redundancy following embryo implantation. Here we define non-redundant roles for TET1 at an early postimplantation stage of the mouse embryo, when its paralogs Tet2 and Tet3 are not detectably expressed. TET1 regulates numerous genes defining differentiation programs in the epiblast and extraembryonic ectoderm. In epiblast cells, TET1 demethylates gene promoters via hydroxymethylation and maintains telomere stability. Surprisingly, TET1 represses a majority of epiblast target genes independently of methylation changes, in part through regulation of the gene encoding the transcriptional repressor JMJD8. Dysregulated gene expression in the absence of TET1 causes embryonic defects, which are partially penetrant in an inbred strain but fully lethal in non-inbred mice. Collectively, our study highlights an interplay between the catalytic and non-catalytic activities of TET1 that is essential for normal development.
[Mh] Termos MeSH primário: Proteínas de Ligação a DNA/fisiologia
Desenvolvimento Embrionário/genética
Proteínas Proto-Oncogênicas/fisiologia
[Mh] Termos MeSH secundário: Animais
Encéfalo/embriologia
Encéfalo/metabolismo
Catálise
Linhagem da Célula
Cruzamentos Genéticos
Metilação de DNA/fisiologia
Proteínas de Ligação a DNA/deficiência
Proteínas de Ligação a DNA/genética
Ectoderma/metabolismo
Gástrula/metabolismo
Dosagem de Genes
Regulação da Expressão Gênica no Desenvolvimento/genética
Técnicas de Inativação de Genes
Camadas Germinativas/metabolismo
Idade Gestacional
Histona Desmetilases com o Domínio Jumonji/biossíntese
Histona Desmetilases com o Domínio Jumonji/genética
Camundongos
Camundongos Endogâmicos C57BL
Células-Tronco Pluripotentes/metabolismo
Proteínas Proto-Oncogênicas/deficiência
Proteínas Proto-Oncogênicas/genética
Deleção de Sequência
Homeostase do Telômero/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA-Binding Proteins); 0 (Proto-Oncogene Proteins); 0 (TET1 protein, mouse); EC 1.14.11.- (JMJD8 protein, mouse); EC 1.14.11.- (Jumonji Domain-Containing Histone Demethylases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170516
[St] Status:MEDLINE
[do] DOI:10.1038/ng.3868


  7 / 3252 MEDLINE  
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[PMID]:28432215
[Au] Autor:Heer NC; Miller PW; Chanet S; Stoop N; Dunkel J; Martin AC
[Ad] Endereço:Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142, USA.
[Ti] Título:Actomyosin-based tissue folding requires a multicellular myosin gradient.
[So] Source:Development;144(10):1876-1886, 2017 05 15.
[Is] ISSN:1477-9129
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Tissue folding promotes three-dimensional (3D) form during development. In many cases, folding is associated with myosin accumulation at the apical surface of epithelial cells, as seen in the vertebrate neural tube and the ventral furrow. This type of folding is characterized by constriction of apical cell surfaces, and the resulting cell shape change is thought to cause tissue folding. Here, we use quantitative microscopy to measure the pattern of transcription, signaling, myosin activation and cell shape in the mesoderm. We found that cells within the ventral domain accumulate different amounts of active apical non-muscle myosin 2 depending on the distance from the ventral midline. This gradient in active myosin depends on a newly quantified gradient in upstream signaling proteins. A 3D continuum model of the embryo with induced contractility demonstrates that contractility gradients, but not contractility per se, promote changes to surface curvature and folding. As predicted by the model, experimental broadening of the myosin domain disrupts tissue curvature where myosin is uniform. Our data argue that apical contractility gradients are important for tissue folding.
[Mh] Termos MeSH primário: Actomiosina/fisiologia
Gástrula/citologia
Gástrula/metabolismo
Gastrulação
Morfogênese/fisiologia
Miosinas/metabolismo
[Mh] Termos MeSH secundário: Citoesqueleto de Actina/metabolismo
Actomiosina/metabolismo
Animais
Animais Geneticamente Modificados
Forma Celular
Drosophila/embriologia
Drosophila/genética
Drosophila/metabolismo
Embrião não Mamífero
Gastrulação/genética
Miosinas/química
Concentração Osmolar
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
9013-26-7 (Actomyosin); EC 3.6.4.1 (Myosins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171126
[Lr] Data última revisão:
171126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170423
[St] Status:MEDLINE
[do] DOI:10.1242/dev.146761


  8 / 3252 MEDLINE  
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[PMID]:28389228
[Au] Autor:Rodriguez AM; Jin DX; Wolfe AD; Mikedis MM; Wierenga L; Hashmi MP; Viebahn C; Downs KM
[Ad] Endereço:Department of Cell and Regenerative Biology, University of Wisconsin-Madison School of Medicine and Public Health, Madison, WI, USA.
[Ti] Título:Brachyury drives formation of a distinct vascular branchpoint critical for fetal-placental arterial union in the mouse gastrula.
[So] Source:Dev Biol;425(2):208-222, 2017 05 15.
[Is] ISSN:1095-564X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:How the fetal-placental arterial connection is made and positioned relative to the embryonic body axis, thereby ensuring efficient and directed blood flow to and from the mother during gestation, is not known. Here we use a combination of genetics, timed pharmacological inhibition in living mouse embryos, and three-dimensional modeling to link two novel architectural features that, at present, have no status in embryological atlases. The allantoic core domain (ACD) is the extraembryonic extension of the primitive streak into the allantois, or pre-umbilical tissue; the vessel of confluence (VOC), situated adjacent to the ACD, is an extraembryonic vessel that marks the site of fetal-placental arterial union. We show that genesis of the fetal-placental connection involves the ACD and VOC in a series of steps, each one dependent upon the last. In the first, Brachyury (T) ensures adequate extension of the primitive streak into the allantois, which in turn designates the allantoic-yolk sac junction. Next, the streak-derived ACD organizes allantoic angioblasts to the axial junction; upon signaling from Fibroblast Growth Factor Receptor-1 (FGFR1), these endothelialize and branch, forming a sprouting VOC that unites the umbilical and omphalomesenteric arteries with the fetal dorsal aortae. Arterial union is followed by the appearance of the medial umbilical roots within the VOC, which in turn designate the correct axial placement of the lateral umbilical roots/common iliac arteries. In addition, we show that the ACD and VOC are conserved across Placentalia, including humans, underscoring their fundamental importance in mammalian biology. We conclude that T is required for correct axial positioning of the VOC via the primitive streak/ACD, while FGFR1, through its role in endothelialization and branching, further patterns it. Together, these genetic, molecular and structural elements safeguard the fetus against adverse outcomes that can result from vascular mispatterning of the fetal-placental arterial connection.
[Mh] Termos MeSH primário: Artérias/embriologia
Proteínas Fetais/metabolismo
Feto/embriologia
Gástrula/irrigação sanguínea
Gástrula/metabolismo
Morfogênese
Placenta/embriologia
Proteínas com Domínio T-Box/metabolismo
[Mh] Termos MeSH secundário: Alantoide/embriologia
Alantoide/metabolismo
Animais
Artérias/metabolismo
Endotélio Vascular/metabolismo
Feminino
Feto/metabolismo
Gástrula/embriologia
Camundongos
Modelos Biológicos
Placenta/metabolismo
Gravidez
Linha Primitiva/embriologia
Linha Primitiva/metabolismo
Receptor Tipo 1 de Fator de Crescimento de Fibroblastos/metabolismo
Artérias Umbilicais/embriologia
Artérias Umbilicais/metabolismo
Remodelação Vascular
Saco Vitelino/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Brachyury protein); 0 (Fetal Proteins); 0 (T-Box Domain Proteins); EC 2.7.10.1 (Receptor, Fibroblast Growth Factor, Type 1)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171110
[Lr] Data última revisão:
171110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170409
[St] Status:MEDLINE


  9 / 3252 MEDLINE  
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[PMID]:28322735
[Au] Autor:Wolfe AD; Rodriguez AM; Downs KM
[Ad] Endereço:Department of Pediatrics, Division of Pediatric Hematology, Oncology & Bone Marrow Transplant, University of Wisconsin-Madison School of Medicine and Public Health, 1111 Highland Avenue, 4105 WIMR, Madison, WI 53705, United States.
[Ti] Título:STELLA collaborates in distinct mesendodermal cell subpopulations at the fetal-placental interface in the mouse gastrula.
[So] Source:Dev Biol;425(1):44-57, 2017 05 01.
[Is] ISSN:1095-564X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The allantois-derived umbilical component of the chorio-allantoic placenta shuttles fetal blood to and from the chorion, thereby ensuring fetal-maternal exchange. The progenitor populations that establish and supply the fetal-umbilical interface lie, in part, within the base of the allantois, where the germ line is claimed to segregate from the soma. Results of recent studies in the mouse have reported that STELLA (DPPA-3, PGC7) co-localizes with PRDM1 (BLIMP1), the bimolecular signature of putative primordial germ cells (PGCs) throughout the fetal-placental interface. Thus, if PGCs form extragonadally within the posterior region of the mammal, they cannot be distinguished from the soma on the basis of these proteins. We used immunohistochemistry, immunofluorescence, and confocal microscopy of the mouse gastrula to co-localize STELLA with a variety of gene products, including pluripotency factor OCT-3/4, mesendoderm-associated T and MIXl1, mesendoderm- and endoderm-associated FOXa2 and hematopoietic factor Runx1. While a subpopulation of cells localizing OCT-3/4 was always found independently of STELLA, STELLA always co-localized with OCT-3/4. Despite previous reports that T is involved in specification of the germ line, co-localization of STELLA and T was detected only in a small subset of cells in the base of the allantois. Slightly later in the hindgut lip, STELLA+/(OCT-3/4+) co-localized with FOXa2, as well as with RUNX1, indicative of definitive endoderm and hemangioblasts, respectively. STELLA was never found with MIXl1. On the basis of these and previous results, we conclude that STELLA identifies at least five distinct cell subpopulations within the allantois and hindgut, where they may be involved in mesendodermal differentiation and hematopoiesis at the posterior embryonic-extraembryonic interface. These data provide a new point of departure for understanding STELLA's potential roles in building the fetal-placental connection.
[Mh] Termos MeSH primário: Embrião de Mamíferos/metabolismo
Endoderma/metabolismo
Gástrula/metabolismo
Proteínas Repressoras/metabolismo
[Mh] Termos MeSH secundário: Alantoide/citologia
Alantoide/embriologia
Alantoide/metabolismo
Animais
Subunidade alfa 2 de Fator de Ligação ao Core/metabolismo
Embrião de Mamíferos/citologia
Embrião de Mamíferos/embriologia
Endoderma/citologia
Endoderma/embriologia
Feminino
Proteínas Fetais/metabolismo
Feto/embriologia
Feto/metabolismo
Gástrula/embriologia
Fator 3-beta Nuclear de Hepatócito/metabolismo
Proteínas de Homeodomínio/metabolismo
Imuno-Histoquímica
Camundongos
Microscopia Confocal
Fator 3 de Transcrição de Octâmero/metabolismo
Placenta/embriologia
Placenta/metabolismo
Fator 1 de Ligação ao Domínio I Regulador Positivo
Gravidez
Proteínas com Domínio T-Box/metabolismo
Fatores de Transcrição/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Brachyury protein); 0 (Core Binding Factor Alpha 2 Subunit); 0 (Dppa3 protein, mouse); 0 (Fetal Proteins); 0 (Foxa2 protein, mouse); 0 (Homeodomain Proteins); 0 (Mixl1 protein, mouse); 0 (Octamer Transcription Factor-3); 0 (Prdm1 protein, mouse); 0 (Repressor Proteins); 0 (Runx1 protein, mouse); 0 (T-Box Domain Proteins); 0 (Transcription Factors); 135845-92-0 (Hepatocyte Nuclear Factor 3-beta); EC 2.1.1.- (Positive Regulatory Domain I-Binding Factor 1)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170322
[St] Status:MEDLINE


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[PMID]:28292844
[Au] Autor:Simunovic M; Brivanlou AH
[Ad] Endereço:Center for Studies in Physics and Biology, The Rockefeller University, New York, NY 10065, USA.
[Ti] Título:Embryoids, organoids and gastruloids: new approaches to understanding embryogenesis.
[So] Source:Development;144(6):976-985, 2017 03 15.
[Is] ISSN:1477-9129
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Cells have an intrinsic ability to self-assemble and self-organize into complex and functional tissues and organs. By taking advantage of this ability, embryoids, organoids and gastruloids have recently been generated , providing a unique opportunity to explore complex embryological events in a detailed and highly quantitative manner. Here, we examine how such approaches are being used to answer fundamental questions in embryology, such as how cells self-organize and assemble, how the embryo breaks symmetry, and what controls timing and size in development. We also highlight how further improvements to these exciting technologies, based on the development of quantitative platforms to precisely follow and measure subcellular and molecular events, are paving the way for a more complete understanding of the complex events that help build the human embryo.
[Mh] Termos MeSH primário: Corpos Embrioides/fisiologia
Desenvolvimento Embrionário
Gástrula/embriologia
Organoides/embriologia
[Mh] Termos MeSH secundário: Animais
Tamanho Corporal
Seres Humanos
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171126
[Lr] Data última revisão:
171126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170316
[St] Status:MEDLINE
[do] DOI:10.1242/dev.143529



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