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Pesquisa : A16.567 [Categoria DeCS]
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  1 / 1081 MEDLINE  
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[PMID]:29273679
[Au] Autor:Rodríguez-Carballo E; Lopez-Delisle L; Zhan Y; Fabre PJ; Beccari L; El-Idrissi I; Huynh THN; Ozadam H; Dekker J; Duboule D
[Ad] Endereço:Department of Genetics and Evolution, University of Geneva, 1205 Geneva, Switzerland.
[Ti] Título:The cluster is a dynamic and resilient TAD boundary controlling the segregation of antagonistic regulatory landscapes.
[So] Source:Genes Dev;31(22):2264-2281, 2017 11 15.
[Is] ISSN:1549-5477
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The mammalian cluster lies between two topologically associating domains (TADs) matching distinct enhancer-rich regulatory landscapes. During limb development, the telomeric TAD controls the early transcription of genes in forearm cells, whereas the centromeric TAD subsequently regulates more posterior genes in digit cells. Therefore, the TAD boundary prevents the terminal gene from responding to forearm enhancers, thereby allowing proper limb patterning. To assess the nature and function of this CTCF-rich DNA region in embryos we compared chromatin interaction profiles between proximal and distal limb bud cells isolated from mutant stocks where various parts of this boundary region were removed. The resulting progressive release in boundary effect triggered inter-TAD contacts, favored by the activity of the newly accessed enhancers. However, the boundary was highly resilient, and only a 400-kb deletion, including the whole-gene cluster, was eventually able to merge the neighboring TADs into a single structure. In this unified TAD, both proximal and distal limb enhancers nevertheless continued to work independently over a targeted transgenic reporter construct. We propose that the whole cluster is a dynamic TAD border and that the exact boundary position varies depending on both the transcriptional status and the developmental context.
[Mh] Termos MeSH primário: Genes Homeobox
Família Multigênica
Sequências Reguladoras de Ácido Nucleico
[Mh] Termos MeSH secundário: Animais
Fator de Ligação a CCCTC/metabolismo
Proteínas de Ciclo Celular/metabolismo
Proteínas Cromossômicas não Histona/metabolismo
Elementos Facilitadores Genéticos
Botões de Extremidades/metabolismo
Camundongos
Deleção de Sequência
Transcrição Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (CCCTC-Binding Factor); 0 (Cell Cycle Proteins); 0 (Chromosomal Proteins, Non-Histone); 0 (Ctcf protein, mouse); 0 (cohesins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180202
[Lr] Data última revisão:
180202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171224
[St] Status:MEDLINE
[do] DOI:10.1101/gad.307769.117


  2 / 1081 MEDLINE  
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[PMID]:27770257
[Au] Autor:Saha A; Rolfe R; Carroll S; Kelly DJ; Murphy P
[Ad] Endereço:Department of Zoology, School of Natural Sciences, Trinity College, Dublin, Ireland.
[Ti] Título:Chondrogenesis of embryonic limb bud cells in micromass culture progresses rapidly to hypertrophy and is modulated by hydrostatic pressure.
[So] Source:Cell Tissue Res;368(1):47-59, 2017 04.
[Is] ISSN:1432-0878
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Chondrogenesis in vivo is precisely controlled in time and space. The entire limb skeleton forms from cells at the core of the early limb bud that condense and undergo chondrogenic differentiation. Whether they form stable cartilage at the articular surface of the joint or transient cartilage that progresses to hypertrophy as endochondral bone, replacing the cartilage template of the skeletal rudiment, is spatially controlled over several days in the embryo. Here, we follow the differentiation of cells taken from the early limb bud (embryonic day 11.5), grown in high-density micromass culture and show that a self-organising pattern of evenly spaced cartilage nodules occurs spontaneously in growth medium. Although chondrogenesis is enhanced by addition of BMP6 to the medium, the spatial pattern of nodule formation is disrupted. We show rapid progression of the entire nodule to hypertrophy in culture and therefore loss of the local signals required to direct formation of stable cartilage. Dynamic hydrostatic pressure, which we have previously predicted to be a feature of the forming embryonic joint region, had a stabilising effect on chondrogenesis, reducing expression of hypertrophic marker genes. This demonstrates the use of micromass culture as a relatively simple assay to compare the effect of both biophysical and molecular signals on spatial and temporal control of chondrogenesis that could be used to examine the response of different types of progenitor cell, both adult- and embryo-derived.
[Mh] Termos MeSH primário: Técnicas de Cultura de Células/métodos
Condrogênese
Pressão Hidrostática
Botões de Extremidades/citologia
Botões de Extremidades/embriologia
[Mh] Termos MeSH secundário: Animais
Diferenciação Celular/genética
Células Cultivadas
Condrogênese/genética
Regulação da Expressão Gênica no Desenvolvimento
Hipertrofia
Camundongos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1705
[Cu] Atualização por classe:180123
[Lr] Data última revisão:
180123
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161023
[St] Status:MEDLINE
[do] DOI:10.1007/s00441-016-2512-9


  3 / 1081 MEDLINE  
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[PMID]:28467430
[Au] Autor:Yokoyama S; Furukawa S; Kitada S; Mori M; Saito T; Kawakami K; Belmonte JCI; Kawakami Y; Ito Y; Sato T; Asahara H
[Ad] Endereço:Department of Systems Biomedicine, National Institute of Child Health and Development, Setagaya, Tokyo, Japan.
[Ti] Título:Analysis of transcription factors expressed at the anterior mouse limb bud.
[So] Source:PLoS One;12(5):e0175673, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Limb bud patterning, outgrowth, and differentiation are precisely regulated in a spatio-temporal manner through integrated networks of transcription factors, signaling molecules, and downstream genes. However, the exact mechanisms that orchestrate morphogenesis of the limb remain to be elucidated. Previously, we have established EMBRYS, a whole-mount in situ hybridization database of transcription factors. Based on the findings from EMBRYS, we focused our expression pattern analysis on a selection of transcription factor genes that exhibit spatially localized and temporally dynamic expression patterns with respect to the anterior-posterior axis in the E9.5-E11.5 limb bud. Among these genes, Irx3 showed a posteriorly expanded expression domain in Shh-/- limb buds and an anteriorly reduced expression domain in Gli3-/- limb buds, suggesting their importance in anterior-posterior patterning. To assess the stepwise EMBRYS-based screening system for anterior regulators, we generated Irx3 transgenic mice in which Irx3 was expressed in the entire limb mesenchyme under the Prrx1 regulatory element. The Irx3 gain-of-function model displayed complex phenotypes in the autopods, including digit loss, radial flexion, and fusion of the metacarpal bones, suggesting that Irx3 may contribute to the regulation of limb patterning, especially in the autopods. Our results demonstrate that gene expression analysis based on EMBRYS could contribute to the identification of genes that play a role in patterning of the limb mesenchyme.
[Mh] Termos MeSH primário: Botões de Extremidades/metabolismo
Fatores de Transcrição/metabolismo
[Mh] Termos MeSH secundário: Animais
Perfilação da Expressão Gênica
Hibridização In Situ
Camundongos
Camundongos Transgênicos
Reação em Cadeia da Polimerase em Tempo Real
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Transcription Factors)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171222
[Lr] Data última revisão:
171222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0175673


  4 / 1081 MEDLINE  
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[PMID]:28743862
[Au] Autor:Farlie PG; Davidson NM; Baker NL; Raabus M; Roeszler KN; Hirst C; Major A; Mariette MM; Lambert DM; Oshlack A; Smith CA
[Ad] Endereço:Murdoch Children's Research Institute, Royal Children's Hospital, Parkville, VIC, 3052, Australia. peter.farlie@mcri.edu.au.
[Ti] Título:Co-option of the cardiac transcription factor Nkx2.5 during development of the emu wing.
[So] Source:Nat Commun;8(1):132, 2017 07 25.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The ratites are a distinctive clade of flightless birds, typified by the emu and ostrich that have acquired a range of unique anatomical characteristics since diverging from basal Aves at least 100 million years ago. The emu possesses a vestigial wing with a single digit and greatly reduced forelimb musculature. However, the embryological basis of wing reduction and other anatomical changes associated with loss of flight are unclear. Here we report a previously unknown co-option of the cardiac transcription factor Nkx2.5 to the forelimb in the emu embryo, but not in ostrich, or chicken and zebra finch, which have fully developed wings. Nkx2.5 is expressed in emu limb bud mesenchyme and maturing wing muscle, and mis-expression of Nkx2.5 throughout the limb bud in chick results in wing reductions. We propose that Nkx2.5 functions to inhibit early limb bud expansion and later muscle growth during development of the vestigial emu wing.The transcription factor Nkx2.5 is essential for heart development. Here, the authors identify a previously unknown expression domain for Nkx2.5 in the emu wing and explore its role in diminished wing bud development in the flightless emu, compared with three other birds that have functional wings.
[Mh] Termos MeSH primário: Proteínas Aviárias/genética
Proteína Homeobox Nkx-2.5/genética
Fatores de Transcrição/genética
Asas de Animais/metabolismo
[Mh] Termos MeSH secundário: Animais
Proteínas Aviárias/metabolismo
Dromaiidae
Membro Anterior/embriologia
Membro Anterior/metabolismo
Perfilação da Expressão Gênica/métodos
Regulação da Expressão Gênica no Desenvolvimento
Hibridização In Situ
Botões de Extremidades/embriologia
Botões de Extremidades/metabolismo
Mesoderma/embriologia
Mesoderma/metabolismo
Músculo Esquelético/embriologia
Músculo Esquelético/metabolismo
Miocárdio/metabolismo
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Asas de Animais/embriologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Avian Proteins); 0 (Homeobox Protein Nkx-2.5); 0 (Transcription Factors)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-00112-7


  5 / 1081 MEDLINE  
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[PMID]:28669818
[Au] Autor:Hopyan S
[Ad] Endereço:Program in Developmental and Stem Cell Biology, Research Institute, The Hospital for Sick Children, Toronto, ON, Canada M5G 0A4; Department of Molecular Genetics, University of Toronto, Canada M5S 1A8; Division of Orthopaedic Surgery, Hospital for Sick Children and University of Toronto, Canada M5G 1X8. Electronic address: sevan.hopyan@sickkids.ca.
[Ti] Título:Biophysical regulation of early limb bud morphogenesis.
[So] Source:Dev Biol;429(2):429-433, 2017 09 15.
[Is] ISSN:1095-564X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The physical basis of morphogenesis is a fascinating concern that has been a longstanding interest of developmental biologists. In this review, I attempt to incorporate earlier and recent biophysical concepts and data to explain basic features of early limb bud morphogenesis. In particular, I discuss the influence of mesenchymal cohesion and physical properties that might contribute to phase separation of the bud from the lateral plate, the possibility that the early dorsoventral limb bud axis is moulded by the surface ectoderm, and endogenous electric fields that might contribute to oriented cell movements which generate the early limb bud. A combination of quantitative biophysical experimentation and modelling will likely advance this field.
[Mh] Termos MeSH primário: Fenômenos Biofísicos
Botões de Extremidades/embriologia
Botões de Extremidades/fisiologia
Morfogênese
[Mh] Termos MeSH secundário: Animais
Movimento Celular
Polaridade Celular
Eletricidade
Botões de Extremidades/citologia
Mesoderma/citologia
Mesoderma/embriologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171111
[Lr] Data última revisão:
171111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170704
[St] Status:MEDLINE


  6 / 1081 MEDLINE  
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[PMID]:28576769
[Au] Autor:Firulli BA; Milliar H; Toolan KP; Harkin J; Fuchs RK; Robling AG; Firulli AB
[Ad] Endereço:Riley Heart Research Center, Herman B Wells Center for Pediatric Research, Departments of Anatomy and Cell Biology, Biochemistry, Medical and Molecular Genetics, Indiana University School of Medicine.
[Ti] Título:Defective Hand1 phosphoregulation uncovers essential roles for Hand1 in limb morphogenesis.
[So] Source:Development;144(13):2480-2489, 2017 07 01.
[Is] ISSN:1477-9129
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The morphogenesis of the vertebrate limbs is a complex process in which cell signaling and transcriptional regulation coordinate diverse structural adaptations in diverse species. In this study, we examine the consequences of altering Hand1 dimer choice regulation within developing vertebrate limbs. Although deletion via the limb-specific reveals a non-essential role for Hand1 in mouse limb morphogenesis, altering Hand1 phosphoregulation, and consequently Hand1 dimerization affinities, results in a severe truncation of proximal-anterior limb elements. Molecular analysis reveals a non-cell-autonomous mechanism that causes widespread cell death within the embryonic limb bud. In addition, we observe changes in proximal-anterior gene regulation, including a reduction in the expression of , , and , all of which are upregulated in limb conditional knockouts. A reduction of and gene dosage improves the integrity of anterior limb structures, validating the importance of the Twist-family bHLH dimer pool in limb morphogenesis.
[Mh] Termos MeSH primário: Fatores de Transcrição Hélice-Alça-Hélice Básicos/metabolismo
Botões de Extremidades/embriologia
Botões de Extremidades/metabolismo
Morfogênese
[Mh] Termos MeSH secundário: Alelos
Animais
Fatores de Transcrição Hélice-Alça-Hélice Básicos/genética
Padronização Corporal/genética
Morte Celular/genética
Feminino
Deleção de Genes
Dosagem de Genes
Regulação da Expressão Gênica no Desenvolvimento
Proteínas Hedgehog/metabolismo
Proteínas de Homeodomínio/metabolismo
Integrases/metabolismo
Masculino
Mesoderma/metabolismo
Camundongos
Mutação/genética
Fenótipo
Fosforilação
Transdução de Sinais/genética
Transcrição Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Basic Helix-Loop-Helix Transcription Factors); 0 (Hand1 protein, mouse); 0 (Hand2 protein, mouse); 0 (Hedgehog Proteins); 0 (Homeodomain Proteins); 0 (Prrx1 protein, mouse); 0 (Shh protein, mouse); EC 2.7.7.- (Cre recombinase); EC 2.7.7.- (Integrases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171126
[Lr] Data última revisão:
171126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170604
[St] Status:MEDLINE
[do] DOI:10.1242/dev.149963


  7 / 1081 MEDLINE  
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[PMID]:28333962
[Au] Autor:Kawata M; Taniguchi Y; Mori D; Yano F; Ohba S; Chung UI; Shimogori T; Mills AA; Tanaka S; Saito T
[Ad] Endereço:Sensory & Motor System Medicine, Faculty of Medicine, The University of Tokyo. 7-3-1 Hongo, Bunkyo-ku, Tokyo, Japan.
[Ti] Título:Different regulation of limb development by p63 transcript variants.
[So] Source:PLoS One;12(3):e0174122, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The apical ectodermal ridge (AER), located at the distal end of each limb bud, is a key signaling center which controls outgrowth and patterning of the proximal-distal axis of the limb through secretion of various molecules. Fibroblast growth factors (FGFs), particularly Fgf8 and Fgf4, are representative molecules produced by AER cells, and essential to maintain the AER and cell proliferation in the underlying mesenchyme, meanwhile Jag2-Notch pathway negatively regulates the AER and limb development. p63, a transcription factor of the p53 family, is expressed in the AER and indispensable for limb formation. However, the underlying mechanisms and specific roles of p63 variants are unknown. Here, we quantified the expression of p63 variants in mouse limbs from embryonic day (E) 10.5 to E12.5, and found that ΔNp63γ was strongly expressed in limbs at all stages, while TAp63γ expression was rapidly increased in the later stages. Fluorescence-activated cell sorting analysis of limb bud cells from reporter mouse embryos at E11.5 revealed that all variants were abundantly expressed in AER cells, and their expression was very low in mesenchymal cells. We then generated AER-specific p63 knockout mice by mating mice with a null and a flox allele of p63, and Msx2-Cre mice (Msx2-Cre;p63Δ/fl). Msx2-Cre;p63Δ/fl neonates showed limb malformation that was more obvious in distal elements. Expression of various AER-related genes was decreased in Msx2-Cre;p63Δ/fl limb buds and embryoid bodies formed by p63-knockdown induced pluripotent stem cells. Promoter analyses and chromatin immunoprecipitation assays demonstrated Fgf8 and Fgf4 as transcriptional targets of ΔNp63γ, and Jag2 as that of TAp63γ. Furthermore, TAp63γ overexpression exacerbated the phenotype of Msx2-Cre;p63Δ/fl mice. These data indicate that ΔNp63 and TAp63 control limb development through transcriptional regulation of different target molecules with different roles in the AER. Our findings contribute to further understanding of the molecular network of limb development.
[Mh] Termos MeSH primário: Botões de Extremidades/crescimento & desenvolvimento
Fosfoproteínas/fisiologia
Transativadores/fisiologia
[Mh] Termos MeSH secundário: Animais
Animais Recém-Nascidos
Fator 4 de Crescimento de Fibroblastos/fisiologia
Fator 8 de Crescimento de Fibroblasto/fisiologia
Regulação da Expressão Gênica no Desenvolvimento/fisiologia
Botões de Extremidades/fisiologia
Camundongos/embriologia
Camundongos Endogâmicos C57BL
Camundongos Knockout
Fosfoproteínas/genética
Reação em Cadeia da Polimerase em Tempo Real
Transativadores/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fgf4 protein, mouse); 0 (Fgf8 protein, mouse); 0 (Fibroblast Growth Factor 4); 0 (Phosphoproteins); 0 (Trans-Activators); 0 (Trp63 protein, mouse); 148997-75-5 (Fibroblast Growth Factor 8)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170822
[Lr] Data última revisão:
170822
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170324
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0174122


  8 / 1081 MEDLINE  
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[PMID]:28235582
[Au] Autor:Bryant DM; Sousounis K; Farkas JE; Bryant S; Thao N; Guzikowski AR; Monaghan JR; Levin M; Whited JL
[Ad] Endereço:Harvard Medical School, the Harvard stem Cell Institute, and the Department of Orthopedic Surgery, Brigham & Women's Hospital, Cambridge, MA 02139, USA.
[Ti] Título:Repeated removal of developing limb buds permanently reduces appendage size in the highly-regenerative axolotl.
[So] Source:Dev Biol;424(1):1-9, 2017 04 01.
[Is] ISSN:1095-564X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Matching appendage size to body size is fundamental to animal function. Generating an appropriately-sized appendage is a robust process executed during development which is also critical for regeneration. When challenged, larger animals are programmed to regenerate larger limbs than smaller animals within a single species. Understanding this process has important implications for regenerative medicine. To approach this complex question, models with altered appendage size:body size ratios are required. We hypothesized that repeatedly challenging axolotls to regrow limb buds would affect their developmental program resulting in altered target morphology. We discovered that after 10 months following this experimental procedure, limbs that developed were permanently miniaturized. This altered target morphology was preserved upon amputation and regeneration. Future experiments using this platform should provide critical information about how target limb size is encoded within limb progenitors.
[Mh] Termos MeSH primário: Ambystoma mexicanum/embriologia
Amputação
Botões de Extremidades/embriologia
Botões de Extremidades/patologia
[Mh] Termos MeSH secundário: Animais
Ectromelia/patologia
Botões de Extremidades/anormalidades
Botões de Extremidades/inervação
Tecido Nervoso/patologia
Tamanho do Órgão
Regeneração
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171110
[Lr] Data última revisão:
171110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170226
[St] Status:MEDLINE


  9 / 1081 MEDLINE  
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[PMID]:28205287
[Au] Autor:Matsubara H; Saito D; Abe G; Yokoyama H; Suzuki T; Tamura K
[Ad] Endereço:Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University, Aobayama Aoba-ku, Sendai, 980-8578, Japan.
[Ti] Título:Upstream regulation for initiation of restricted Shh expression in the chick limb bud.
[So] Source:Dev Dyn;246(5):417-430, 2017 May.
[Is] ISSN:1097-0177
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The organizing center, which serves as a morphogen source, has crucial functions in morphogenesis in animal development. The center is necessarily located in a certain restricted area in the morphogenetic field, and there are several ways in which an organizing center can be restricted. The organizing center for limb morphogenesis, the ZPA (zone of polarizing activity), specifically expresses the Shh gene and is restricted to the posterior region of the developing limb bud. RESULTS: The pre-pattern along the limb anteroposterior axis, provided by anterior Gli3 expression and posterior Hand2 expression, seems insufficient for the initiation of Shh expression restricted to a narrow, small spot in the posterior limb field. Comparison of the spatiotemporal patterns of gene expression between Shh and some candidate genes (Fgf8, Hoxd10, Hoxd11, Tbx2, and Alx4) upstream of Shh expression suggested that a combination of these genes' expression provides the restricted initiation of Shh expression. CONCLUSIONS: Taken together with results of functional assays, we propose a model in which positive and negative transcriptional regulatory networks accumulate their functions in the intersection area of their expression regions to provide a restricted spot for the ZPA, the source of morphogen, Shh. Developmental Dynamics 246:417-430, 2017. © 2017 Wiley Periodicals, Inc.
[Mh] Termos MeSH primário: Regulação da Expressão Gênica no Desenvolvimento
Redes Reguladoras de Genes/fisiologia
Proteínas Hedgehog/genética
Botões de Extremidades/metabolismo
[Mh] Termos MeSH secundário: Animais
Embrião de Galinha
Proteínas Hedgehog/fisiologia
Morfogênese
Organizadores Embrionários
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hedgehog Proteins)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170526
[Lr] Data última revisão:
170526
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170217
[St] Status:MEDLINE
[do] DOI:10.1002/dvdy.24493


  10 / 1081 MEDLINE  
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[PMID]:28192166
[Au] Autor:Saad K; Theis S; Otto A; Luke G; Patel K
[Ad] Endereço:School of Biological Sciences, University of Reading, Reading, UK.
[Ti] Título:Detailed expression profile of the six Glypicans and their modifying enzyme, Notum during chick limb and feather development.
[So] Source:Gene;610:71-79, 2017 Apr 30.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The development of vertebrate appendages, especially the limb and feather buds are orchestrated by numerous secreted signalling molecules including Sonic Hedgehog, Bone Morphogenetic Proteins, Fibroblast Growth Factors and Wnts. These proteins coordinate the growth and patterning of ectodermal and mesenchymal cells. The influence of signalling molecules is affected over large distances by their concentration (morphogen activity) but also at local levels by the presence of proteins that either attenuate or promote their activity. Glypicans are cell surface molecules that regulate the activity of the major secreted signalling molecules expressed in the limb and feather bud. Here we investigated the expression of all Glypicans during chick limb and feather development. In addition we profiled the expression of Notum, an enzyme that regulates Glypican activity. We show that five of the six Glypicans and Notum are expressed in a dynamic manner during the development of limbs and feathers. We also investigated the expression of key Glypicans and show that they are controlled by signalling molecules highlighting the presence of feedback loops. Lastly we show that Glypicans and Notum are expressed in a tissue specific manner in adult chicken tissues. Our results strongly suggest that the Glypicans and Notum have many as yet undiscovered roles to play during the development of vertebrate appendages.
[Mh] Termos MeSH primário: Embrião de Galinha/metabolismo
Esterases/metabolismo
Glipicanas/metabolismo
[Mh] Termos MeSH secundário: Animais
Proteínas Morfogenéticas Ósseas/metabolismo
Embrião de Galinha/enzimologia
Galinhas
Extremidades/crescimento & desenvolvimento
Plumas/crescimento & desenvolvimento
Retroalimentação
Feminino
Fatores de Crescimento de Fibroblastos/metabolismo
Botões de Extremidades/metabolismo
Mesoderma/metabolismo
Transdução de Sinais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bone Morphogenetic Proteins); 0 (Glypicans); 62031-54-3 (Fibroblast Growth Factors); EC 3.1.- (Esterases)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170315
[Lr] Data última revisão:
170315
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170214
[St] Status:MEDLINE



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