Base de dados : MEDLINE
Pesquisa : A16.675 [Categoria DeCS]
Referências encontradas : 375 [refinar]
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[PMID]:28887017
[Au] Autor:Henry JQ; Lyons DC; Perry KJ; Osborne CC
[Ad] Endereço:University of Illinois, Department of Cell&Developmental Biology, 601 S. Goodwin Ave., Urbana, IL 61801, United States. Electronic address: j-henry4@illinois.edu.
[Ti] Título:Establishment and activity of the D quadrant organizer in the marine gastropod Crepidula fornicata.
[So] Source:Dev Biol;431(2):282-296, 2017 11 15.
[Is] ISSN:1095-564X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:During development in metazoan embryos, the fundamental embryonic axes are established by organizing centers that influence the fates of nearby cells. Among the spiralians, a large and diverse branch of protostome metazoans, studies have shown that an organizer sets up the dorsal-ventral axis, which arises from one of the four basic cell quadrants during development (the dorsal, D quadrant). Studies in a few species have also revealed variation in terms of how and when the D quadrant and the organizer are established. In some species the D quadrant is specified conditionally, via cell-cell interactions, while in others it is specified autonomously, via asymmetric cell divisions (such as those involving the formation of polar lobes). The third quartet macromere (3D) typically serves as the spiralian organizer; however, other cells born earlier or later in the D quadrant lineage can serve as the organizer, such as the 2d micromere in the annelid Capitella teleta or the 4d micromere in the mollusc Crepidula fornicata. Here we present work carried out in the snail C. fornicata to show that establishment of a single D quadrant appears to rely on a combination of both autonomous (via inheritance of the polar lobe) and conditional mechanisms (involving induction via the progeny of the first quartet micromeres). Through systematic ablation of cells, we show that D quadrant identity is established between 5th and 6th cleavage stages, as it is in other spiralians that use conditional specification. Subsequently, following the next cell cycle, organizer activity takes place soon after the birth of the 4d micromere. Therefore, unlike the case in other spiralians that use conditional specification, the specification of the D quadrant and the activity of the dorso-ventral organizer are temporally and spatially uncoupled. We also present data on organizer function in naturally-occurring and experimentally-induced twin embryos, which possess multiple D quadrants. We show that supernumerary D quadrants can arise in C. fornicata (either spontaneously or following polar lobe removal); when multiple D quadrants are present these do not exhibit effective organizer activity. We conclude that the polar lobe is not required for D quadrant specification, though it could play a role in effective organizer activity. We also tested whether the inheritance of the small polar lobe by the D quadrant is associated with the ability to laterally inhibit neighboring quadrants by direct contact in order to normally prevent supernumerary organizers from arising. Finally, we discuss the variation of spiralian organizers in a phylogenetic context.
[Mh] Termos MeSH primário: Organismos Aquáticos/citologia
Organismos Aquáticos/crescimento & desenvolvimento
Gastrópodes/citologia
Gastrópodes/embriologia
Organizadores Embrionários/citologia
Organizadores Embrionários/embriologia
[Mh] Termos MeSH secundário: Animais
Fase de Clivagem do Zigoto/citologia
Embrião não Mamífero/citologia
Embrião não Mamífero/metabolismo
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171111
[Lr] Data última revisão:
171111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170910
[St] Status:MEDLINE


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[PMID]:28302747
[Au] Autor:Rong X; Zhou Y; Liu Y; Zhao B; Wang B; Wang C; Gong X; Tang P; Lu L; Li Y; Zhao C; Zhou J
[Ad] Endereço:Key Laboratory of Marine Drugs (Ocean University of China), Chinese Ministry of Education, and School of Medicine and Pharmacy, Ocean University of China, 5 Yushan Road, Qingdao 266003, China.
[Ti] Título:Glutathione peroxidase 4 inhibits Wnt/ß-catenin signaling and regulates dorsal organizer formation in zebrafish embryos.
[So] Source:Development;144(9):1687-1697, 2017 05 01.
[Is] ISSN:1477-9129
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The Wnt/ß-catenin signaling pathway plays pivotal roles in axis formation during embryogenesis and in adult tissue homeostasis. Glutathione peroxidase 4 (GPX4) is a selenoenzyme and participates in the reduction of peroxides. Its synthesis depends on the availability of the element selenium. However, the roles of GPX4 in vertebrate embryonic development and underlying mechanisms are largely unknown. Here, we show that maternal loss of zebrafish promotes embryonic dorsal organizer formation, whereas overexpression of inhibits the development of the dorsal organizer. Depletion of human and zebrafish ( / ) increases, while / overexpression decreases, Wnt/ß-catenin signaling and Functional and epistatic studies showed that GPX4 functions at the Tcf/Lef level, independently of selenocysteine activation. Mechanistically, GPX4 interacts with Tcf/Lefs and inhibits Wnt activity by preventing the binding of Tcf/Lefs to the promoters of Wnt target genes, resulting in inhibitory action in the presence of Wnt/ß-catenin signaling. Our findings unravel GPX4 as a suppressor of Wnt/ß-catenin signals, suggesting a possible relationship between the Wnt/ß-catenin pathway and selenium via the association of Tcf/Lef family proteins with GPX4.
[Mh] Termos MeSH primário: Embrião não Mamífero/enzimologia
Glutationa Peroxidase/metabolismo
Organizadores Embrionários/enzimologia
Via de Sinalização Wnt
Proteínas de Peixe-Zebra/metabolismo
Peixe-Zebra/embriologia
Peixe-Zebra/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Sequência de Bases
Sistemas CRISPR-Cas/genética
Embrião não Mamífero/citologia
Evolução Molecular
Regulação da Expressão Gênica no Desenvolvimento
Glutationa Peroxidase/química
Glutationa Peroxidase/deficiência
Células HEK293
Seres Humanos
Fenótipo
Regiões Promotoras Genéticas/genética
Ligação Proteica/genética
Selênio/metabolismo
Transdução de Sinais/genética
Transcrição Genética
Proteínas de Peixe-Zebra/química
Proteínas de Peixe-Zebra/genética
Zigoto/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Zebrafish Proteins); EC 1.11.1.12 (phospholipid-hydroperoxide glutathione peroxidase); EC 1.11.1.9 (Glutathione Peroxidase); H6241UJ22B (Selenium)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171126
[Lr] Data última revisão:
171126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170318
[St] Status:MEDLINE
[do] DOI:10.1242/dev.144261


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[PMID]:28205287
[Au] Autor:Matsubara H; Saito D; Abe G; Yokoyama H; Suzuki T; Tamura K
[Ad] Endereço:Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University, Aobayama Aoba-ku, Sendai, 980-8578, Japan.
[Ti] Título:Upstream regulation for initiation of restricted Shh expression in the chick limb bud.
[So] Source:Dev Dyn;246(5):417-430, 2017 May.
[Is] ISSN:1097-0177
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The organizing center, which serves as a morphogen source, has crucial functions in morphogenesis in animal development. The center is necessarily located in a certain restricted area in the morphogenetic field, and there are several ways in which an organizing center can be restricted. The organizing center for limb morphogenesis, the ZPA (zone of polarizing activity), specifically expresses the Shh gene and is restricted to the posterior region of the developing limb bud. RESULTS: The pre-pattern along the limb anteroposterior axis, provided by anterior Gli3 expression and posterior Hand2 expression, seems insufficient for the initiation of Shh expression restricted to a narrow, small spot in the posterior limb field. Comparison of the spatiotemporal patterns of gene expression between Shh and some candidate genes (Fgf8, Hoxd10, Hoxd11, Tbx2, and Alx4) upstream of Shh expression suggested that a combination of these genes' expression provides the restricted initiation of Shh expression. CONCLUSIONS: Taken together with results of functional assays, we propose a model in which positive and negative transcriptional regulatory networks accumulate their functions in the intersection area of their expression regions to provide a restricted spot for the ZPA, the source of morphogen, Shh. Developmental Dynamics 246:417-430, 2017. © 2017 Wiley Periodicals, Inc.
[Mh] Termos MeSH primário: Regulação da Expressão Gênica no Desenvolvimento
Redes Reguladoras de Genes/fisiologia
Proteínas Hedgehog/genética
Botões de Extremidades/metabolismo
[Mh] Termos MeSH secundário: Animais
Embrião de Galinha
Proteínas Hedgehog/fisiologia
Morfogênese
Organizadores Embrionários
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hedgehog Proteins)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170526
[Lr] Data última revisão:
170526
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170217
[St] Status:MEDLINE
[do] DOI:10.1002/dvdy.24493


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[PMID]:27919666
[Au] Autor:De Almeida I; Oliveira NM; Randall RA; Hill CS; McCoy JM; Stern CD
[Ad] Endereço:Department of Cell & Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK.
[Ti] Título:Calreticulin is a secreted BMP antagonist, expressed in Hensen's node during neural induction.
[So] Source:Dev Biol;421(2):161-170, 2017 Jan 15.
[Is] ISSN:1095-564X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Hensen's node is the "organizer" of the avian and mammalian early embryo. It has many functions, including neural induction and patterning of the ectoderm and mesoderm. Some of the signals responsible for these activities are known but these do not explain the full complexity of organizer activity. Here we undertake a functional screen to discover new secreted factors expressed by the node at this time of development. Using a Signal Sequence Trap in yeast, we identify several candidates. Here we focus on Calreticulin. We show that in addition to its known functions in intracellular Calcium regulation and protein folding, Calreticulin is secreted, it can bind to BMP4 and act as a BMP antagonist in vivo and in vitro. Calreticulin is not sufficient to account for all organizer functions but may contribute to the complexity of its activity.
[Mh] Termos MeSH primário: Proteínas Morfogenéticas Ósseas/antagonistas & inibidores
Calreticulina/metabolismo
Indução Embrionária
Tecido Nervoso/embriologia
Tecido Nervoso/metabolismo
Organizadores Embrionários/metabolismo
[Mh] Termos MeSH secundário: Animais
Proteínas Morfogenéticas Ósseas/metabolismo
Calnexina/metabolismo
Galinhas
Fatores de Crescimento de Fibroblastos/antagonistas & inibidores
Fatores de Crescimento de Fibroblastos/metabolismo
Células HEK293
Seres Humanos
Placa Neural/embriologia
Placa Neural/metabolismo
Transdução de Sinais
Solubilidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bone Morphogenetic Proteins); 0 (Calreticulin); 139873-08-8 (Calnexin); 62031-54-3 (Fibroblast Growth Factors)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161207
[St] Status:MEDLINE


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[PMID]:27914912
[Au] Autor:Stauber M; Weidemann M; Dittrich-Breiholz O; Lobschat K; Alten L; Mai M; Beckers A; Kracht M; Gossler A
[Ad] Endereço:Institut für Molekularbiologie, OE 5250, Medizinische Hochschule Hannover, Carl-Neuberg-Str. 1, 30625 Hannover, Germany. Electronic address: Stauber.Michael@mh-hannover.de.
[Ti] Título:Identification of FOXJ1 effectors during ciliogenesis in the foetal respiratory epithelium and embryonic left-right organiser of the mouse.
[So] Source:Dev Biol;423(2):170-188, 2017 03 15.
[Is] ISSN:1095-564X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Formation of motile cilia in vertebrate embryos is essential for proper development and tissue function. Key regulators of motile ciliogenesis are the transcription factors FOXJ1 and NOTO, which are conserved throughout vertebrates. Downstream target genes of FOXJ1 have been identified in a variety of species, organs and cultured cell lines; in murine embryonic and foetal tissues, however, FOXJ1 and NOTO effectors have not been comprehensively analysed and our knowledge of the downstream genetic programme driving motile ciliogenesis in the mammalian lung and ventral node is fragmentary. We compared genome-wide expression profiles of undifferentiated E14.5 vs. abundantly ciliated E18.5 micro-dissected airway epithelia as well as Foxj1 vs. Foxj1-deficient foetal (E16.5) lungs of the mouse using microarray hybridisation. 326 genes deregulated in both screens are candidates for FOXJ1-dependent, ciliogenesis-associated factors at the endogenous onset of motile ciliogenesis in the lung, including 123 genes that have not been linked to ciliogenesis before; 46% of these novel factors lack known homologues outside mammals. Microarray screening of Noto vs. Noto null early headfold embryos (E7.75) identified 59 of the lung candidates as NOTO/FOXJ1-dependent factors in the embryonic left-right organiser that carries a different subtype of motile cilia. For several uncharacterised factors from this small overlap - including 1700012B09Rik, 1700026L06Rik and Fam183b - we provide extended experimental evidence for a ciliary function.
[Mh] Termos MeSH primário: Cílios/metabolismo
Feto/metabolismo
Fatores de Transcrição Forkhead/metabolismo
Organizadores Embrionários/metabolismo
Organogênese
Mucosa Respiratória/embriologia
[Mh] Termos MeSH secundário: Animais
Diferenciação Celular/genética
Linhagem Celular
Regulação para Baixo/genética
Regulação da Expressão Gênica no Desenvolvimento
Ontologia Genética
Estudos de Associação Genética
Genoma
Proteínas de Fluorescência Verde/metabolismo
Pulmão/embriologia
Pulmão/metabolismo
Camundongos
Especificidade de Órgãos/genética
Organogênese/genética
Reprodutibilidade dos Testes
Mucosa Respiratória/citologia
Frações Subcelulares/metabolismo
Transcriptoma/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (FOXJ1 protein, mouse); 0 (Forkhead Transcription Factors); 147336-22-9 (Green Fluorescent Proteins)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171110
[Lr] Data última revisão:
171110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161205
[St] Status:MEDLINE


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[PMID]:27522305
[Au] Autor:Haramoto Y; Saijyo T; Tanaka T; Furuno N; Suzuki A; Ito Y; Kondo M; Taira M; Takahashi S
[Ad] Endereço:Biotechnology Research Institute for Drug Discovery, National Institute of Advanced Industrial Science and Technology (AIST), Central 5, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
[Ti] Título:Identification and comparative analyses of Siamois cluster genes in Xenopus laevis and tropicalis.
[So] Source:Dev Biol;426(2):374-383, 2017 06 15.
[Is] ISSN:1095-564X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Two siamois-related homeobox genes siamois (sia1) and twin (sia2), have been reported in Xenopus laevis. These genes are expressed in the blastula chordin- and noggin-expressing (BCNE) center and the Nieuwkoop center, and have complete secondary axis-inducing activity when over-expressed on the ventral side of the embryo. Using whole genome sequences of X. tropicalis and X. laevis, we identified two additional siamois-related genes, which are tandemly duplicated near sia1 and sia2 to form the siamois gene cluster. Four siamois genes in X. tropicalis are transcribed at blastula to gastrula stages. In X. laevis, the siamois gene cluster is present on both homeologous chromosomes, XLA3L and XLA3S. Transcripts from seven siamois genes (three on XLA3L and four on XLA3S) in X. laevis were detected at blastula to gastrula stages. A transcribed gene, sia1p. S, encodes an inactive protein without a homeodomain. When over-expressed ventrally, all siamois-related genes tested in this study except for sia1p. S induced a complete secondary axis, indicating that X. tropicalis and X. laevis have four and six active siamois-related genes, respectively. Of note, each gene required different amounts of mRNA for full activity. These results suggest the possibility that siamois cluster genes have functional redundancy to endow robustness and quickness to organizer formation in Xenopus species.
[Mh] Termos MeSH primário: Proteínas de Homeodomínio/genética
Família Multigênica
Proteínas de Xenopus/genética
Xenopus/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Blástula/metabolismo
Padronização Corporal/genética
Mapeamento Cromossômico
Sequência Conservada
Diploide
Embrião não Mamífero/metabolismo
Gástrula/metabolismo
Dosagem de Genes
Regulação da Expressão Gênica no Desenvolvimento
Organizadores Embrionários
Alinhamento de Sequência
Homologia de Sequência de Aminoácidos
Especificidade da Espécie
Tetraploidia
Via de Sinalização Wnt
Xenopus/embriologia
Xenopus laevis/embriologia
Xenopus laevis/genética
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Homeodomain Proteins); 0 (Xenopus Proteins); 0 (siamois protein, Xenopus)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171110
[Lr] Data última revisão:
171110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160815
[St] Status:MEDLINE


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[PMID]:27209239
[Au] Autor:Popov IK; Kwon T; Crossman DK; Crowley MR; Wallingford JB; Chang C
[Ad] Endereço:Department of Cell, Developmental and Integrative Biology, University of Alabama at Birmingham, Birmingham, AL 35294, United States.
[Ti] Título:Identification of new regulators of embryonic patterning and morphogenesis in Xenopus gastrulae by RNA sequencing.
[So] Source:Dev Biol;426(2):429-441, 2017 06 15.
[Is] ISSN:1095-564X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:During early vertebrate embryogenesis, cell fate specification is often coupled with cell acquisition of specific adhesive, polar and/or motile behaviors. In Xenopus gastrulae, tissues fated to form different axial structures display distinct motility. The cells in the early organizer move collectively and directionally toward the animal pole and contribute to anterior mesendoderm, whereas the dorsal and the ventral-posterior trunk tissues surrounding the blastopore of mid-gastrula embryos undergo convergent extension and convergent thickening movements, respectively. While factors regulating cell lineage specification have been described in some detail, the molecular machinery that controls cell motility is not understood in depth. To gain insight into the gene battery that regulates both cell fates and motility in particular embryonic tissues, we performed RNA sequencing (RNA-seq) to investigate differentially expressed genes in the early organizer, the dorsal and the ventral marginal zone of Xenopus gastrulae. We uncovered many known signaling and transcription factors that have been reported to play roles in embryonic patterning during gastrulation. We also identified many uncharacterized genes as well as genes that encoded extracellular matrix (ECM) proteins or potential regulators of actin cytoskeleton. Co-expression of a selected subset of the differentially expressed genes with activin in animal caps revealed that they had distinct ability to block activin-induced animal cap elongation. Most of these factors did not interfere with mesodermal induction by activin, but an ECM protein, EFEMP2, inhibited activin signaling and acted downstream of the activated type I receptor. By focusing on a secreted protein kinase PKDCC1, we showed with overexpression and knockdown experiments that PKDCC1 regulated gastrulation movements as well as anterior neural patterning during early Xenopus development. Overall, our studies identify many differentially expressed signaling and cytoskeleton regulators in different embryonic regions of Xenopus gastrulae and imply their functions in regulating cell fates and/or behaviors during gastrulation.
[Mh] Termos MeSH primário: Padronização Corporal/genética
Gástrula/metabolismo
Regulação da Expressão Gênica no Desenvolvimento/genética
Análise de Sequência de RNA
Xenopus/genética
[Mh] Termos MeSH secundário: Ativinas/fisiologia
Animais
Embrião não Mamífero/metabolismo
Embrião não Mamífero/ultraestrutura
Proteínas da Matriz Extracelular/fisiologia
Gástrula/ultraestrutura
Camadas Germinativas/metabolismo
Morfogênese/genética
Organizadores Embrionários
Proteínas Tirosina Quinases/fisiologia
RNA Mensageiro/biossíntese
RNA Mensageiro/genética
Xenopus/embriologia
Proteínas de Xenopus/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Extracellular Matrix Proteins); 0 (RNA, Messenger); 0 (Xenopus Proteins); 104625-48-1 (Activins); EC 2.7.10.1 (Pkdcc1 protein, Xenopus); EC 2.7.10.1 (Protein-Tyrosine Kinases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171110
[Lr] Data última revisão:
171110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160523
[St] Status:MEDLINE


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[PMID]:27016259
[Au] Autor:Ding Y; Colozza G; Zhang K; Moriyama Y; Ploper D; Sosa EA; Benitez MDJ; De Robertis EM
[Ad] Endereço:Howard Hughes Medical Institute and Department of Biological Chemistry, University of California, Los Angeles, CA 90095-1662, USA.
[Ti] Título:Genome-wide analysis of dorsal and ventral transcriptomes of the Xenopus laevis gastrula.
[So] Source:Dev Biol;426(2):176-187, 2017 06 15.
[Is] ISSN:1095-564X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:RNA sequencing has allowed high-throughput screening of differential gene expression in many tissues and organisms. Xenopus laevis is a classical embryological and cell-free extract model system, but its genomic sequence had been lacking due to difficulties arising from allotetraploidy. There is currently much excitement surrounding the release of the completed X. laevis genome (version 9.1) by the Joint Genome Institute (JGI), which provides a platform for genome-wide studies. Here we present a deep RNA-seq dataset of transcripts expressed in dorsal and ventral lips of the early Xenopus gastrula embryo using the new genomic information, which was further annotated by blast searches against the human proteome. Overall, our findings confirm previous results from differential screenings using other methods that uncovered classical dorsal genes such as Chordin, Noggin and Cerberus, as well as ventral genes such as Sizzled, Ventx, Wnt8 and Bambi. Complete transcriptome-wide tables of mRNAs suitable for data mining are presented, which include many novel dorsal- and ventral-specific genes. RNA-seq was very quantitative and reproducible, and allowed us to define dorsal and ventral signatures useful for gene set expression analyses (GSEA). As an example of a new gene, we present here data on an organizer-specific secreted protein tyrosine kinase known as Pkdcc (protein kinase domain containing, cytoplasmic) or Vlk (vertebrate lonesome kinase). Overexpression experiments indicate that Pkdcc can act as a negative regulator of Wnt/ ß-catenin signaling independently of its kinase activity. We conclude that RNA-Seq in combination with the X. laevis complete genome now available provides a powerful tool for unraveling cell-cell signaling pathways during embryonic induction.
[Mh] Termos MeSH primário: Padronização Corporal/genética
Gástrula/metabolismo
Regulação da Expressão Gênica no Desenvolvimento
Transcriptoma
Xenopus laevis/embriologia
[Mh] Termos MeSH secundário: Animais
Embrião não Mamífero/metabolismo
Etiquetas de Sequências Expressas
Biblioteca Gênica
Cabeça/embriologia
Microinjeções
Organizadores Embrionários/metabolismo
Proteínas Tirosina Quinases/biossíntese
Proteínas Tirosina Quinases/genética
RNA/genética
RNA Mensageiro/biossíntese
RNA Mensageiro/genética
Análise de Sequência de RNA
Via de Sinalização Wnt
Proteínas de Xenopus/biossíntese
Proteínas de Xenopus/genética
Xenopus laevis/genética
Xenopus laevis/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Messenger); 0 (Xenopus Proteins); 63231-63-0 (RNA); EC 2.7.10.1 (Pkdcc1 protein, Xenopus); EC 2.7.10.1 (Pkdcc2 protein, Xenopus); EC 2.7.10.1 (Protein-Tyrosine Kinases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171110
[Lr] Data última revisão:
171110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160327
[St] Status:MEDLINE


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[PMID]:27287807
[Au] Autor:Zhang J; Jiang Z; Liu X; Meng A
[Ad] Endereço:State Key Laboratory of Membrane Biology, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China.
[Ti] Título:Eph/ephrin signaling maintains the boundary of dorsal forerunner cell cluster during morphogenesis of the zebrafish embryonic left-right organizer.
[So] Source:Development;143(14):2603-15, 2016 07 15.
[Is] ISSN:1477-9129
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The Kupffer's vesicle (KV) is the so-called left-right organizer in teleost fishes. KV is formed from dorsal forerunner cells (DFCs) and generates asymmetrical signals for breaking symmetry of embryos. It is unclear how DFCs or KV cells are prevented from intermingling with adjacent cells. In this study, we show that the Eph receptor gene ephb4b is highly expressed in DFCs whereas ephrin ligand genes, including efnb2b, are expressed in cells next to the DFC cluster during zebrafish gastrulation. ephb4b knockdown or mutation and efnb2b knockdown cause dispersal of DFCs, a smaller KV and randomization of laterality organs. DFCs often dynamically form lamellipodium-like, bleb-like and filopodium-like membrane protrusions at the interface, which attempt to invade but are bounced back by adjacent non-DFC cells during gastrulation. Upon inhibition of Eph/ephrin signaling, however, the repulsion between DFCs and non-DFC cells is weakened or lost, allowing DFCs to migrate away. Ephb4b/Efnb2b signaling by activating RhoA activity mediates contact and repulsion between DFCs and neighboring cells during gastrulation, preventing intermingling of different cell populations. Therefore, our data uncover an important role of Eph/ephrin signaling in maintaining DFC cluster boundary and KV boundary for normal left-right asymmetrical development.
[Mh] Termos MeSH primário: Padronização Corporal
Embrião não Mamífero/citologia
Efrinas/metabolismo
Morfogênese
Organizadores Embrionários/citologia
Receptor EphB4/metabolismo
Transdução de Sinais
Proteínas de Peixe-Zebra/metabolismo
Peixe-Zebra/embriologia
[Mh] Termos MeSH secundário: Animais
Agregação Celular
Comunicação Celular
Movimento Celular
Embrião não Mamífero/metabolismo
Lateralidade Funcional
Técnicas de Inativação de Genes
Mesoderma/citologia
Mutação/genética
Organizadores Embrionários/metabolismo
Proteínas rho de Ligação ao GTP/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Ephrins); 0 (Zebrafish Proteins); EC 2.7.10.1 (Receptor, EphB4); EC 3.6.5.2 (rho GTP-Binding Proteins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171125
[Lr] Data última revisão:
171125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160612
[St] Status:MEDLINE
[do] DOI:10.1242/dev.132969


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[PMID]:27171818
[Au] Autor:Kim S; Zhao Y; Lee JM; Kim WR; Gorivodsky M; Westphal H; Geum D
[Ad] Endereço:1 Department of Biomedical Sciences, Korea University Medical School , Seoul, South Korea .
[Ti] Título:Ldb1 Is Essential for the Development of Isthmic Organizer and Midbrain Dopaminergic Neurons.
[So] Source:Stem Cells Dev;25(13):986-94, 2016 Jul 01.
[Is] ISSN:1557-8534
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:LIM domain-binding protein 1 (Ldb1) is a nuclear cofactor that interacts with LIM homeodomain proteins to form multiprotein complexes that are important for transcription regulation. Ldb1 has been shown to play essential roles in various processes during mouse embryogenesis. To determine the role of Ldb1 in mid- and hindbrain development, we have generated a conditional mutant with a specific deletion of the Ldb1 in the Engrailed-1-expressing region of the developing mid- and hindbrain. Our study showed that the deletion impaired the expression of signaling molecules, such as fibroblast growth factor 8 (FGF8) and Wnt1, in the isthmic organizer and the expression of Shh in the ventral midbrain. The midbrain and the cerebellum were severely reduced in size, and the midbrain dopaminergic (mDA) neurons were missing in the mutant. These defects are identical to the phenotype that has been observed previously in mice with a deletion of the LIM homeodomain gene Lmx1b. Our results thus provide genetic evidence supporting that Ldb1 and Lmx1b function cooperatively to regulate mid- and hindbrain development. In addition, we found that mouse embryonic stem cells lacking Ldb1 failed to generate several types of differentiated neurons, including the mDA neurons, serotonergic neurons, cholinergic neurons, and olfactory bulb neurons, indicating an essential cell-autonomous role for Ldb1 in the development of these neurons.
[Mh] Termos MeSH primário: Proteínas de Ligação a DNA/metabolismo
Neurônios Dopaminérgicos/citologia
Neurônios Dopaminérgicos/metabolismo
Proteínas com Domínio LIM/metabolismo
Mesencéfalo/citologia
Organizadores Embrionários/embriologia
Organizadores Embrionários/metabolismo
[Mh] Termos MeSH secundário: Animais
Biomarcadores/metabolismo
Diferenciação Celular
Cerebelo/embriologia
Embrião de Mamíferos/metabolismo
Regulação da Expressão Gênica no Desenvolvimento
Mesencéfalo/embriologia
Camundongos
Células-Tronco Embrionárias Murinas/citologia
Células-Tronco Embrionárias Murinas/metabolismo
Mutação/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (DNA-Binding Proteins); 0 (LIM Domain Proteins); 0 (Ldb1 protein, mouse)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170911
[Lr] Data última revisão:
170911
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160513
[St] Status:MEDLINE
[do] DOI:10.1089/scd.2015.0307



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