Base de dados : MEDLINE
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  1 / 323 MEDLINE  
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[PMID]:28939335
[Au] Autor:Gonçalves WG; Fernandes KM; Santana WC; Martins GF; Zanuncio JC; Serrão JE
[Ad] Endereço:Departamento de Biologia Geral, Universidade Federal de Viçosa, 36570-900 Viçosa, Minas Gerais, Brazil. Electronic address: wagner.gonzaga@ufv.br.
[Ti] Título:Post-embryonic changes in the hindgut of honeybee Apis mellifera workers: Morphology, cuticle deposition, apoptosis, and cell proliferation.
[So] Source:Dev Biol;431(2):194-204, 2017 11 15.
[Is] ISSN:1095-564X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In insects, the hindgut is a homeostatic region of the digestive tract, divided into pylorus, ileum, and rectum, that reabsorbs water, ions, and small molecules produced during hemolymph filtration. The hindgut anatomy in bee larvae is different from that of adult workers. This study reports the morphological changes and cellular events that occur in the hindgut during the metamorphosis of the honeybee Apis mellifera. We describe the occurrence of autophagosomes and the ultrastructure of the epithelial cells and cuticle, suggesting that cuticular degradation begins in prepupae, with the cuticle being reabsorbed and recycled by autophagosomes in white- and pink-eyed pupae, followed by the deposition of new cuticle in light-brown-eyed pupae. In L5S larvae and prepupae, the hindgut undergoes cell proliferation in the anterior and posterior ends. In the pupae, the pylorus, ileum, and rectum regions are differentiated, and cell proliferation ceases in dark-brown-eyed pupae. Apoptosis occurs in the hindgut from the L5S larval to the pink-eyed pupal stage. In light-brown- and dark-brown-eyed pupae, the ileum epithelium changes from pseudostratified to simple only after the production of the basal lamina, whereas the rectal epithelium is always flattened. In black-eyed pupae, ileum epithelial cells have large vacuoles and subcuticular spaces, while in adult forager workers these cells have long invaginations in the cell apex and many mitochondria, indicating a role in the transport of compounds. Our findings show that hindgut morphogenesis is a dynamic process, with tissue remodeling and cellular events taking place for the formation of different regions of the organ, the reconstruction of a new cuticle, and the remodeling of visceral muscles.
[Mh] Termos MeSH primário: Apoptose
Abelhas/anatomia & histologia
Abelhas/embriologia
Sistema Digestório/citologia
Sistema Digestório/embriologia
Hierarquia Social
Tegumento Comum/anatomia & histologia
[Mh] Termos MeSH secundário: Animais
Autofagia
Abelhas/ultraestrutura
Caspase 3/metabolismo
Proliferação Celular
Sistema Digestório/ultraestrutura
Histonas/metabolismo
Larva/citologia
Larva/ultraestrutura
Pupa/citologia
Pupa/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Histones); EC 3.4.22.- (Caspase 3)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171111
[Lr] Data última revisão:
171111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170924
[St] Status:MEDLINE


  2 / 323 MEDLINE  
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[PMID]:28873446
[Au] Autor:Vanícková L; Nagy R; Pompeiano A; Kalinová B
[Ad] Endereço:Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Brno, Czech Republic.
[Ti] Título:Epicuticular chemistry reinforces the new taxonomic classification of the Bactrocera dorsalis species complex (Diptera: Tephritidae, Dacinae).
[So] Source:PLoS One;12(9):e0184102, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bactrocera invadens Drew, Tsuruta & White, Bactrocera papayae Drew & Hancock, and Bactrocera philippinensis Drew & Hancock, key pest species within the Bactrocera dorsalis species complex, have been recently synonymized under the name Bactrocera dorsalis (Hendel). The closely related Bactrocera carambolae Drew & Hancock remains as a discrete taxonomic entity. Although the synonymizations have been accepted by most researchers, debate about the species limits remains. Because of the economic importance of this group of taxa, any new information available to support or deny the synonymizations is valuable. We investigated the chemical epicuticle composition of males and females of B. dorsalis, B. invadens, B. papayae, B. philippinensis, and B. carambolae by means of one- and two-dimensional gas chromatography-mass spectrometry, followed by multiple factor analyses and principal component analysis. Clear segregation of complex cuticule profiles of both B. carambolae sexes from B. dorsalis (Hendel) was observed. In addition to cuticular hydrocarbons, abundant complex mixtures of sex-specific oxygenated lipids (three fatty acids and 22 fatty acid esters) with so far unknown function were identified in epicuticle extracts from females of all species. The data obtained supports both taxonomic synonymization of B. invadens, B. papayae, and B. philippinensis with B. dorsalis, as well as the exclusion of B. carambolae from B. dorsalis.
[Mh] Termos MeSH primário: Tegumento Comum/fisiologia
Tephritidae/química
Tephritidae/classificação
[Mh] Termos MeSH secundário: Animais
Análise Fatorial
Cromatografia Gasosa-Espectrometria de Massas
Hidrocarbonetos/análise
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hydrocarbons)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170906
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184102


  3 / 323 MEDLINE  
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[PMID]:28441468
[Au] Autor:Plochocki JH; Ruiz S; Rodriguez-Sosa JR; Hall MI
[Ad] Endereço:Department of Anatomy, Midwestern University, Glendale, Arizona, United States of America.
[Ti] Título:Histological study of white rhinoceros integument.
[So] Source:PLoS One;12(4):e0176327, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In this study, we report findings from a microscopic analysis of the white rhinoceros (Ceratotherium simum) integumentary ultrastructure. Skin samples from the cheek, shoulder, flank and rump were taken from a 46-year-old female southern white rhinoceros and examined using H&E and elastic histological stains. The epidermis was thickest in the flank (1.003 mm) followed by the rump, cheek and shoulder. The stratum corneum comprised more than half the epidermal thickness. Numerous melanin granules were found in the basal and spinosum layers. The epidermal-dermal junction was characterized by abundant papillary folds increasing surface contact between integument layers. Most of the dermal thickness consisted of organized collagen bundles with scattered elastic fibers. Collagen fiber bundles were thickest in the flank (210.9 µm) followed by shoulder, rump and cheek. Simple coiled sweat glands were present in the dermis, but hair and sebaceous glands were absent. Together, these data suggest the white rhinoceros has a unique integumentary system among large terrestrial herbivores.
[Mh] Termos MeSH primário: Tegumento Comum/anatomia & histologia
Perissodáctilos/anatomia & histologia
Pele/anatomia & histologia
Glândulas Sudoríparas/anatomia & histologia
[Mh] Termos MeSH secundário: Animais
Colágeno/ultraestrutura
Epiderme/anatomia & histologia
Epiderme/ultraestrutura
Feminino
Pele/ultraestrutura
Glândulas Sudoríparas/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9007-34-5 (Collagen)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170426
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0176327


  4 / 323 MEDLINE  
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[PMID]:28030348
[Au] Autor:Salmani S; Haseli M
[Ti] Título:Prochristianella clarkeae Beveridge, 1990 (Eutetrarhynchidae): a species complex or a species with intraspecific variation in the distribution of its tegumental microtriches?
[So] Source:Acta Parasitol;62(1):69-75, 2017 Mar 01.
[Is] ISSN:1896-1851
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:The microthrix pattern of common forms of the trypanorhynch cestode Prochristianella clarkeae Beveridge, 1990 on which the microscopically visible microtriches almost extended to the posterior end of the scolex was described using SEM and compared to other forms for which this pattern was different. The proximal bothrial surface and the scolex peduncle of all the examined specimens were covered with palmate spinitriches and papilliform filitriches. Unlike the common specimens of P. clarkeae, on the surface of those specimens with unusual distributions of microtriches as well as those on which the microscopically visible microtriches were not observable, palmate spinitriches extended to the mid-pars vaginalis and not to the posterior end of the scolex. Statistical tests did not detect any significant difference in the measurements of the specimens possessing different microthrix patterns. Furthermore, the partial 28S rDNA confirmed that the different forms were conspecific and that P. clarkeae possesses an intraspecific variation in the distribution of palmate spinitriches. Pastinachus sephen as a new host record was also established for Prochristianella clarkeae reported for the first time from the Persian Gulf.
[Mh] Termos MeSH primário: Cestoides/classificação
Cestoides/ultraestrutura
Tegumento Comum/anatomia & histologia
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
DNA Ribossômico/genética
RNA Ribossômico 28S/genética
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Ribosomal); 0 (RNA, Ribosomal, 28S)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170517
[Lr] Data última revisão:
170517
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161229
[St] Status:MEDLINE


  5 / 323 MEDLINE  
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[PMID]:27856284
[Au] Autor:Agez G; Bayon C; Mitov M
[Ad] Endereço:Centre d'Elaboration de Matériaux et d'Etudes Structurales, CEMES, Centre National de la Recherche Scientifique, University Paul-Sabatier, CNRS, UPR 8011, F-31055 Toulouse Cedex 4, France.
[Ti] Título:Multiwavelength micromirrors in the cuticle of scarab beetle Chrysina gloriosa.
[So] Source:Acta Biomater;48:357-367, 2017 Jan 15.
[Is] ISSN:1878-7568
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Beetles from the genus Chrysina show vivid reflections from bright green to metallic silver-gold as a consequence of the cholesteric liquid crystal organization of chitin molecules. Particularly, the cuticle of Chrysina gloriosa exhibits green and silver stripes. By combining confocal microscopy and spectrophotometry, scanning electron microscopy and numerical simulations, the relationship between the reflectance and the structural parameters for both stripes at the micro- and nanoscales are established. Over the visible and near IR spectra, polygonal cells in tessellated green stripes behave as multiwavelength selective micro-mirrors and the silver stripes as specular broadband mirrors. Thermoregulation, conspecifics or intra-species communication, or camouflage against predators are discussed as possible functions. As a prerequisite to bio-inspired artificial replicas, the physical characteristics of the polygonal texture in Chrysina gloriosa cuticle are compared to their equivalents in synthetic cholesteric oligomers and their fundamental differences are ascertained. It is shown that the cuticle has concave cells whereas the artificial films have convex cells, contrary to expectation and assumption in the literature. The present results may provide inspiration for fabricating multiwavelength selective micromirrors or spatial wavelength-specific light modulators. STATEMENT OF SIGNIFICANCE: Many insects own a tessellated carapace with bumps, pits or indentations. Little is known on the physical properties of these geometric variations and biological functions are unknown or still debated. We show that the polygonal cells in scarab beetle Chrysina gloriosa behave as multiwavelength selective micromirrors over the visible and infrared spectra, with a variety of spatial patterns. In the context of biomimetic materials, we demonstrate that the carapace has concave cells whereas the artificial films have convex cells, contrary to expectation in the literature. Thermoregulation, communication or camouflage are discussed as advanced functions. Results may provide inspiration for fabricating spatial wavelength-specific light modulators and optical packet switching in routing technologies.
[Mh] Termos MeSH primário: Coleópteros/anatomia & histologia
Tegumento Comum/anatomia & histologia
Luz
Óptica e Fotônica
[Mh] Termos MeSH secundário: Animais
Coleópteros/ultraestrutura
Simulação por Computador
Pigmentação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161119
[St] Status:MEDLINE


  6 / 323 MEDLINE  
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[PMID]:26931091
[Au] Autor:Chen Y; Dallara PL; Nelson LJ; Coleman TW; Hishinuma SM; Carrillo D; Seybold SJ
[Ad] Endereço:Department of Entomology and Nematology, University of California, Davis, USA.
[Ti] Título:Comparative morphometric and chemical analyses of phenotypes of two invasive ambrosia beetles (Euwallacea spp.) in the United States.
[So] Source:Insect Sci;24(4):647-662, 2017 Aug.
[Is] ISSN:1744-7917
[Cp] País de publicação:Australia
[La] Idioma:eng
[Ab] Resumo:The polyphagous shot hole borer (PSHB), Euwallacea sp., was first detected in 2003 in Los Angeles County, California, USA. Recently, this invasive species has become a major pest of many hardwood trees in urban and wildland forests throughout southern California. PSHB is nearly identical in morphology and life history to the tea shot hole borer (TSHB), Euwallacea fornicatus, an invasive pest of hardwoods in Florida, USA and many other parts of the world. However, molecular studies have suggested that the taxa are different species. We conducted morphometric and chemical analyses of the phenotypes of Euwallacea sp. collected in southern California (Los Angeles County) and E. fornicatus collected in Florida (Miami-Dade County). Our analyses indicated that PSHB has 3 larval instars. The third larval instar was separated from the first 2 instars by head capsule width with 0 probability of misclassification. The body length, head width, and pronotal width of PSHB adult males were significantly less than those of females. Head width and pronotal width of female PSHB were significantly less than those of female TSHB. In contrast, body length, and ratio of body length to pronotal width of female PSHB were significantly greater than those of female TSHB. However, females of these 2 species could not be separated completely by these 4 measurements because of the overlapping ranges. Cuticular hydrocarbons detected in both species were exclusively alkanes (i.e., n-alkanes, monomethylalkanes, dimethylalkanes, and trimethylalkanes). Cuticular hydrocarbon profiles of PSHB males and females were similar, but they both differed from that of TSHB females. Cuticular hydrocarbons of PSHB were predominantly internally branched dimethylalkanes with backbones of 31 and 33 carbons, whereas cuticular hydrocarbons of TSHB females were dominated by internally branched monomethylalkanes and dimethylalkanes with backbones of 28 and 29 carbons. Multiple compounds within these classes appear to be diagnostic for PSHB and TSHB, respectively.
[Mh] Termos MeSH primário: Hidrocarbonetos/análise
Hidrocarbonetos/química
Tegumento Comum
Gorgulhos/anatomia & histologia
Gorgulhos/química
[Mh] Termos MeSH secundário: Alcanos
Animais
California
Feminino
Florida
Espécies Introduzidas
Larva/anatomia & histologia
Larva/crescimento & desenvolvimento
Masculino
Fenótipo
Gorgulhos/classificação
Gorgulhos/crescimento & desenvolvimento
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkanes); 0 (Hydrocarbons)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160303
[St] Status:MEDLINE
[do] DOI:10.1111/1744-7917.12329


  7 / 323 MEDLINE  
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[PMID]:27658600
[Au] Autor:Eraky MA; Aly NS; Selem RF; El-Kholy AA; Rashed GA
[Ad] Endereço:Department Parasitology, Faculty of Medicine, Benha University, Benha 13518, Egypt.
[Ti] Título:In Vitro Schistosomicidal Activity of Phytol and Tegumental Alterations Induced in Juvenile and Adult Stages of Schistosoma haematobium.
[So] Source:Korean J Parasitol;54(4):477-84, 2016 Aug.
[Is] ISSN:1738-0006
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:There is renewed interest in natural products as a starting point for discovery of drugs for schistosomiasis. Recent studies have shown that phytol reveals interesting in vivo and in vitro antischistosomal properties against Schistosoma mansoni adult worms. Here, we report the in vitro antischistosomal activity of phytol against Schistosoma haematobium juvenile and adult worms and alterations on the tegumental surface of the worms by means of scanning electron microscopy. The assay, which was carried out with 6 concentrations (25, 50, 75, 100, 125, and 150 µg/ml) of phytol, has shown a promising activity in a dose and time-dependent manner. There was a significant decline in the motility of the worms and a mortality rate of 100% was found at 48 hr after they had been exposed to phytol in the concentration of 150 µg/ml. Male worms were more susceptible. On the ultrastructural level, phytol also induced tegumental peeling, disintegration of tubercles and spines in addition to morphological disfiguring of the oral and ventral suckers. This report provides the first evidence that phytol is able to kill S. haematobium of different ages, and emphasizes that it is a promising natural product that could be used for development of a new schistosomicidal agent.
[Mh] Termos MeSH primário: Anti-Helmínticos/farmacologia
Tegumento Comum
Fitol/farmacologia
Schistosoma haematobium/efeitos dos fármacos
Schistosoma haematobium/ultraestrutura
[Mh] Termos MeSH secundário: Animais
Relação Dose-Resposta a Droga
Feminino
Locomoção/efeitos dos fármacos
Masculino
Mesocricetus
Microscopia Eletrônica de Varredura
Schistosoma haematobium/fisiologia
Caramujos
Análise de Sobrevida
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthelmintics); 150-86-7 (Phytol)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170404
[Lr] Data última revisão:
170404
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160924
[St] Status:MEDLINE
[do] DOI:10.3347/kjp.2016.54.4.477


  8 / 323 MEDLINE  
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[PMID]:27506161
[Au] Autor:Alibardi L
[Ad] Endereço:Comparative Histolab and Department of Bigea, University of Bologna, Italy. lorenzo.alibardi@unibo.it.
[Ti] Título:Sauropsids Cornification is Based on Corneous Beta-Proteins, a Special Type of Keratin-Associated Corneous Proteins of the Epidermis.
[So] Source:J Exp Zool B Mol Dev Evol;326(6):338-351, 2016 Sep.
[Is] ISSN:1552-5015
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The evolution of the process of cornification in amniote epidermis from the general process of keratinization present in simple epithelia of anamniotes took place through the evolution of specialized intermediate filament (α) keratins, keratin-associated proteins (KAPs) and corneous proteins (CPs). The scanty information on the three-dimensional conformation of known KAPs and CPs indicate these proteins contain α-helix, random coiled, or beta sheets with different lengths and organizations. CP genes originated in a chromosome locus indicated as epidermal differentiation complex (EDC), and transformed the epidermal keratinization of anamniotes into the cornified epidermis and skin appendages of amniotes (claws, beaks, and feathers). In particular, peculiar genes encoding for small proteins with a central region of 34 amino acids conformed as beta sheets were originated in the EDC of sauropsids (reptiles and birds). These proteins were traditionally indicated as beta-keratins because they form filaments of 3-4 nm in diameter and show an X-ray beta pattern. Different from other proteins of the EDC, dimers of these corneous beta-proteins associate into long polymers of filamentous proteins utilized in sauropsids skin appendages, such as scales and feathers. Future challenges in this area of research will be the study on gene regulation and expression for these proteins, their origin and evolution in different lineages of sauropsids, and their role in determining the material properties of sauropsid scales and other skin appendages.
[Mh] Termos MeSH primário: Aves/metabolismo
Epiderme/metabolismo
Répteis/metabolismo
beta-Queratinas/metabolismo
[Mh] Termos MeSH secundário: Animais
Proteínas Aviárias/metabolismo
Evolução Biológica
Aves/anatomia & histologia
Epiderme/anatomia & histologia
Tegumento Comum
Queratinas/metabolismo
Répteis/anatomia & histologia
Proteínas de Répteis/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Avian Proteins); 0 (Reptilian Proteins); 0 (beta-Keratins); 68238-35-7 (Keratins)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160811
[St] Status:MEDLINE
[do] DOI:10.1002/jez.b.22689


  9 / 323 MEDLINE  
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[PMID]:27498800
[Au] Autor:Suzuki N; Sato M; Nassar HF; Abdel-Gawad FKh; Bassem SM; Yachiguchi K; Tabuchi Y; Endo M; Sekiguchi T; Urata M; Hattori A; Mishima H; Shimasaki Y; Oshima Y; Hong CS; Makino F; Tang N; Toriba A; Hayakawa K
[Ad] Endereço:1 Noto Marine Laboratory, Institute of Nature and Environmental Technology, Division of Marine Environmental Studies, Kanazawa University, Housu-gun, Ishikawa 927-0553, Japan.
[Ti] Título:Seawater Polluted with Highly Concentrated Polycyclic Aromatic Hydrocarbons Suppresses Osteoblastic Activity in the Scales of Goldfish, Carassius auratus.
[So] Source:Zoolog Sci;33(4):407-13, 2016 Aug.
[Is] ISSN:0289-0003
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:We have developed an original in vitro bioassay using teleost scale, that has osteoclasts, osteoblasts, and bone matrix as each marker: alkaline phosphatase (ALP) for osteoblasts and tartrate-resistant acid phosphatase (TRAP) for osteoclasts. Using this scale in vitro bioassay, we examined the effects of seawater polluted with highly concentrated polycyclic aromatic hydrocarbons (PAHs) and nitro-polycyclic aromatic hydrocarbons (NPAHs) on osteoblastic and osteoclastic activities in the present study. Polluted seawater was collected from two sites (the Alexandria site on the Mediterranean Sea and the Suez Canal site on the Red Sea). Total levels of PAHs in the seawater from the Alexandria and Suez Canal sites were 1364.59 and 992.56 ng/l, respectively. We were able to detect NPAHs in both seawater samples. Total levels of NPAHs were detected in the seawater of the Alexandria site (12.749 ng/l) and the Suez Canal site (3.914 ng/l). Each sample of polluted seawater was added to culture medium at dilution rates of 50, 100, and 500, and incubated with the goldfish scales for 6 hrs. Thereafter, ALP and TRAP activities were measured. ALP activity was significantly suppressed by both polluted seawater samples diluted at least 500 times, but TRAP activity did not change. In addition, mRNA expressions of osteoblastic markers (ALP, osteocalcin, and the receptor activator of the NF-κB ligand) decreased significantly, as did the ALP enzyme activity. In fact, ALP activity decreased on treatment with PAHs and NPAHs. We conclude that seawater polluted with highly concentrated PAHs and NPAHs influences bone metabolism in teleosts.
[Mh] Termos MeSH primário: Carpa Dourada
Tegumento Comum
Osteoblastos/efeitos dos fármacos
Hidrocarbonetos Aromáticos Policíclicos/toxicidade
Água do Mar/química
Poluentes Químicos da Água/toxicidade
[Mh] Termos MeSH secundário: Animais
Regulação da Expressão Gênica
Marcadores Genéticos
Hidrocarbonetos Aromáticos Policíclicos/química
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Poluentes Químicos da Água/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Genetic Markers); 0 (Polycyclic Aromatic Hydrocarbons); 0 (RNA, Messenger); 0 (Water Pollutants, Chemical)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170127
[Lr] Data última revisão:
170127
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160809
[St] Status:MEDLINE
[do] DOI:10.2108/zs150211


  10 / 323 MEDLINE  
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[PMID]:27307255
[Au] Autor:Duarte AP; Ferro M; Rodrigues A; Bacci M; Nagamoto NS; Forti LC; Pagnocca FC
[Ad] Endereço:Center for the Study of Social Insects, UNESP - São Paulo State University, Avenida 24-A, n. 1515, Bela Vista, Rio Claro, SP, 13.506-900, Brazil. ana.mirandaduarte@gmail.com.
[Ti] Título:Prevalence of the genus Cladosporium on the integument of leaf-cutting ants characterized by 454 pyrosequencing.
[So] Source:Antonie Van Leeuwenhoek;109(9):1235-43, 2016 Sep.
[Is] ISSN:1572-9699
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The relationship of attine ants with their mutualistic fungus and other microorganisms has been studied during the last two centuries. However, previous studies about the diversity of fungi in the ants' microenvironment are based mostly on culture-dependent approaches, lacking a broad characterization of the fungal ant-associated community. Here, we analysed the fungal diversity found on the integument of Atta capiguara and Atta laevigata alate ants using 454 pyrosequencing. We obtained 35,453 ITS reads grouped into 99 molecular operational taxonomic units (MOTUs). Data analysis revealed that A. capiguara drones had the highest diversity of MOTUs. Besides the occurrence of several uncultured fungi, the mycobiota analysis revealed that the most abundant taxa were the Cladosporium-complex, Cryptococcus laurentii and Epicoccum sp. Taxa in the genus Cladosporium were predominant in all samples, comprising 67.9 % of all reads. The remarkable presence of the genus Cladosporium on the integument of leaf-cutting ants alates from distinct ant species suggests that this fungus is favored in this microenvironment.
[Mh] Termos MeSH primário: Formigas/microbiologia
Cladosporium/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Biodiversidade
Brasil
Cladosporium/classificação
Cladosporium/genética
DNA Fúngico/genética
DNA Espaçador Ribossômico/genética
Tegumento Comum/microbiologia
Micobioma
Prevalência
Análise de Sequência de DNA
Pele/microbiologia
Simbiose
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Fungal); 0 (DNA, Ribosomal Spacer)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170309
[Lr] Data última revisão:
170309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160617
[St] Status:MEDLINE
[do] DOI:10.1007/s10482-016-0724-3



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