Base de dados : MEDLINE
Pesquisa : A17.815.180 [Categoria DeCS]
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[PMID]:29413990
[Au] Autor:Kelly J; Murphy JE
[Ad] Endereço:Mitochondrial Biology & Radiation Research Centre, Dept. of Life Sciences, Institute of Technology Sligo, Ash Lane, Sligo, Ireland. Electronic address: janiskelly@mail.itsligo.ie.
[Ti] Título:Mitochondrial gene expression changes in cultured human skin cells following simulated sunlight irradiation.
[So] Source:J Photochem Photobiol B;179:167-174, 2018 Feb.
[Is] ISSN:1873-2682
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Exposure of skin to simulated sunlight irradiation (SSI) has being extensively researched and shown to be the main cause for changes in the skin including changes in cellular function and generation of reactive oxygen species (ROS). This oxidative stress can subsequently exert downstream effects and the subcellular compartments most affected by this oxidative stress are mitochondria. The importance of functional mitochondrial morphology is apparent as morphological defects are related to many human diseases including diabetes mellitus, liver disease, neurodegenerative diseases, aging and cancer. OBJECTIVE: The main objective of this study was to evaluate solar radiation-induced changes in mitochondrial gene expression in human skin cells using a Q-Sun solar simulator to deliver a close match to the intensity of summer sunlight. METHODS: Spontaneously immortalised human skin epidermal keratinocytes (HaCaT) and Human Dermal Fibroblasts (HDFn) were divided into two groups. Group A were irradiated once and Group B twice 7days apart; following irradiation, mitochondrial gene expression was evaluated 1, 4 and 7days post primary exposure for group A and 1, 4, 7 and 14days post-secondary exposure for group B. RESULTS: Both the epidermal and dermal cells displayed significant reduced expression of the genes analysed for mitochondrial morphology and function; however, epidermal cells displayed this reduction post SSI earlier then dermal cells at multiple time points. CONCLUSION: The data presented here reinforces the fact that epidermal cells, while displaying a heightened sensitivity to sunlight, are less prone to changes in gene expression, while dermal cells, which appear to be more resilient are possibly more prone to genomic instability and mitochondrial damage.
[Mh] Termos MeSH primário: Expressão Gênica/efeitos da radiação
Mitocôndrias/genética
Luz Solar
[Mh] Termos MeSH secundário: ATPases Associadas a Diversas Atividades Celulares/genética
ATPases Associadas a Diversas Atividades Celulares/metabolismo
Linhagem Celular
Derme/citologia
Epiderme/citologia
GTP Fosfo-Hidrolases/genética
GTP Fosfo-Hidrolases/metabolismo
Seres Humanos
Queratinócitos/citologia
Queratinócitos/metabolismo
Queratinócitos/efeitos da radiação
Metaloendopeptidases/genética
Metaloendopeptidases/metabolismo
Mitocôndrias/efeitos da radiação
Proteínas de Transporte da Membrana Mitocondrial/genética
Proteínas de Transporte da Membrana Mitocondrial/metabolismo
Proteínas Mitocondriais/genética
Proteínas Mitocondriais/metabolismo
Espécies Reativas de Oxigênio/metabolismo
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Superóxido Dismutase/genética
Superóxido Dismutase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Mitochondrial Membrane Transport Proteins); 0 (Mitochondrial Proteins); 0 (Reactive Oxygen Species); EC 1.15.1.1 (Superoxide Dismutase); EC 1.15.1.1 (superoxide dismutase 2); EC 3.4.24.- (Metalloendopeptidases); EC 3.4.24.- (YME1L1 protein, human); EC 3.6.1.- (GTP Phosphohydrolases); EC 3.6.1.- (MFN2 protein, human); EC 3.6.1.- (OPA1 protein, human); EC 3.6.4.- (ATPases Associated with Diverse Cellular Activities); EC 3.6.5.- (Mfn1 protein, human)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180223
[Lr] Data última revisão:
180223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180208
[St] Status:MEDLINE


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[PMID]:29331373
[Au] Autor:Kwack MH; Yang JM; Won GH; Kim MK; Kim JC; Sung YK
[Ad] Endereço:Department of Immunology, School of Medicine, Kyungpook National University, Daegu, South Korea.
[Ti] Título:Establishment and characterization of five immortalized human scalp dermal papilla cell lines.
[So] Source:Biochem Biophys Res Commun;496(2):346-351, 2018 02 05.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Dermal papilla (DP) regulates the growth and cycling of hair follicles. Cultured DP cells are useful for the study of their role in relation to hair growth and regeneration. However, cultivation of human DP cells is tedious and difficult. In addition, cultured DP cells possess a relatively short replicative life span, requiring immortalized human DP cell lines. We previously established an immortalized human DP cell line, SV40T-hTERT-DPC, by introducing human telomerase reverse transcriptase (hTERT) gene into the transformed cell line, SV40T-DPC. In this study, we co-transfected the simian virus 40 large T antigen (SV40T-Ag) and hTERT into DP cells from scalp hair follicles from a male with androgenetic alopecia and established five immortalized DP cell lines and named KNU-101, KNU-102, KNU-103, KNU-201 and KNU-202. We then evaluated tumorigenicity, expression of DP markers, responses to androgen, Wnt3a and BMP4, and expression of DP signature genes. These cell lines displayed early passage morphology and maintained responses to androgen, Wnt and BMP. Furthermore, these cell lines expressed DP markers and DP signature genes. KNU cell lines established in this study are potentially useful sources for hair research.
[Mh] Termos MeSH primário: Alopecia/genética
Derme/metabolismo
Efeito Fundador
Folículo Piloso/metabolismo
[Mh] Termos MeSH secundário: Células A549
Alopecia/metabolismo
Alopecia/patologia
Animais
Biglicano/genética
Biglicano/metabolismo
Biomarcadores/metabolismo
Proteína Morfogenética Óssea 4/farmacologia
Carcinogênese/genética
Carcinogênese/metabolismo
Carcinogênese/patologia
Linhagem Celular Transformada
Derme/patologia
Di-Hidrotestosterona/farmacologia
Feminino
Expressão Gênica
Folículo Piloso/efeitos dos fármacos
Folículo Piloso/patologia
Seres Humanos
Queratina-8/genética
Queratina-8/metabolismo
Masculino
Camundongos
Camundongos Endogâmicos BALB C
Camundongos Nus
Receptores Androgênicos/genética
Receptores Androgênicos/metabolismo
Couro Cabeludo/metabolismo
Couro Cabeludo/patologia
Versicanas/genética
Versicanas/metabolismo
Proteína Wnt3A/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (BGN protein, human); 0 (BMP4 protein, human); 0 (Biglycan); 0 (Biomarkers); 0 (Bone Morphogenetic Protein 4); 0 (KRT8 protein, human); 0 (Keratin-8); 0 (Receptors, Androgen); 0 (VCAN protein, human); 0 (WNT3A protein, human); 0 (Wnt3A Protein); 08J2K08A3Y (Dihydrotestosterone); 126968-45-4 (Versicans)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180115
[St] Status:MEDLINE


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[PMID]:29288671
[Au] Autor:Zhang Y; Wang Y; Wu G; Zhang W; Wang X; Cai W; Zhang J; Han S; Li Y; Bai X; Shi J; Su L; Hu D
[Ad] Endereço:Department of Burns and Cutaneous Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi Province, 710032, China.
[Ti] Título:Prolonged skin grafts survival time by IFN-γ in allogeneic skin transplantation model during acute rejection through IFN-γ/STAT3/IDO pathway in epidermal layer.
[So] Source:Biochem Biophys Res Commun;496(2):436-442, 2018 02 05.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Allogeneic skin transplantation is the life-saving therapy for multiple diseases, including extensive burn, large-scale trauma and certain post-surgical complications. However, acute rejection impedes clinical application of allogeneic skin transplantation. Although a lot of novel immunosuppressant drugs have been developed, there is still great need for ideal therapy with less complication and more therapeutic effects. Here, we found interferon gamma (IFN-γ) as an immunomodulatory cytokine prolonged the survival time of allografts from (8.50 ±â€¯1.517) days to (14.83 ±â€¯2.714) days at best. Indoleamine-2, 3-dioxygenase (IDO) has been proposed to play key roles in induction of immune tolerance. Using in vitro tissue culture and primary keratinocytes and fibroblasts, we investigated the regulatory effects of IFN-γ on the IDO expression. IFN-γ upregulated IDO expression through STAT3 phosphorylation and this upregulation was reduced by abolition of STAT3 phosphorylation through a STAT3 phosphorylation inhibitor. Interestingly, IFN-γ induced IDO expression predominately in epidermis rather than dermis. In consistent with these results, IFN-γ significantly triggered IDO expression in keratinocytes but not fibroblasts. Taken together, this suggests that IFN-γ might be a potential immunomodulatory drug in acute rejection and keratinocytes in epidermis may play a main role in immune tolerance after allogeneic skin transplantation.
[Mh] Termos MeSH primário: Rejeição de Enxerto/prevenção & controle
Sobrevivência de Enxerto
Fatores Imunológicos/farmacologia
Indolamina-Pirrol 2,3,-Dioxigenase/genética
Interferon gama/farmacologia
Fator de Transcrição STAT3/genética
Transplante de Pele
[Mh] Termos MeSH secundário: Doença Aguda
Animais
Derme/citologia
Derme/efeitos dos fármacos
Derme/imunologia
Epiderme/citologia
Epiderme/efeitos dos fármacos
Epiderme/imunologia
Fibroblastos/citologia
Fibroblastos/efeitos dos fármacos
Fibroblastos/imunologia
Regulação da Expressão Gênica
Rejeição de Enxerto/imunologia
Rejeição de Enxerto/patologia
Tolerância Imunológica/efeitos dos fármacos
Indolamina-Pirrol 2,3,-Dioxigenase/imunologia
Queratinócitos/citologia
Queratinócitos/efeitos dos fármacos
Queratinócitos/imunologia
Masculino
Camundongos
Camundongos Endogâmicos BALB C
Camundongos Endogâmicos C57BL
Especificidade de Órgãos
Fosforilação
Fator de Transcrição STAT3/imunologia
Transplante Homólogo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (IDO1 protein, mouse); 0 (Immunologic Factors); 0 (Indoleamine-Pyrrole 2,3,-Dioxygenase); 0 (STAT3 Transcription Factor); 0 (Stat3 protein, mouse); 82115-62-6 (Interferon-gamma)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180213
[Lr] Data última revisão:
180213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171231
[St] Status:MEDLINE


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[PMID]:29369208
[Au] Autor:Gu C; Wang XX; Luo X; Liu F; Zhou XY; Yang J; Yang Q; Wang X
[Ad] Endereço:Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
[Ti] Título:An alternative strategy treated giant congenital melanocytic nevi with epidermis and superficial dermis of the lesions.
[So] Source:Medicine (Baltimore);97(4):e9725, 2018 Jan.
[Is] ISSN:1536-5964
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Giant congenital melanocytic nevi (GCMN) are defined as rare pigmented lesions that are believed to form between weeks 9 and 20 of gestation. It is difficult to reconstruct large defects after the removal of the lesions and it has posed a great challenge to the plastic surgeon and dermatologist.Given all those difficulty reconstructing the defects, we try to explore an alternative way to resurfacing the defect after removal of GCMN.Patients with GCMN received single-stage excision. Following the subcutaneous tissue and deep dermis were discarded, epidermis and superficial dermis were harvested as graft substitutes to reconstruct the defects in situ.All of the grafted tissue survived well and skin color in the surgical area gradually became lighter. During the periodicity of follow-up, neither hypertrophic scars nor recurrence were observed. Furthermore, histopathology examination demonstrated that there are no distinct melanocytes gathered in the postoperation lesions.For those GCMN which is difficult to reconstruct with traditional methods, resection of the lesion followed by reconstruction with epidermis skin and superficial dermis from the lesions in situ may be a feasible and alternative therapy method.
[Mh] Termos MeSH primário: Derme/transplante
Epiderme/transplante
Nevo Pigmentado/cirurgia
Neoplasias Cutâneas/cirurgia
[Mh] Termos MeSH secundário: Adolescente
Criança
Estudos de Viabilidade
Feminino
Seguimentos
Seres Humanos
Masculino
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180208
[Lr] Data última revisão:
180208
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:180126
[St] Status:MEDLINE
[do] DOI:10.1097/MD.0000000000009725


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[PMID]:29264903
[Au] Autor:Hazarika D; Pawar M
[Ad] Endereço:Department of Dermatology, Gauhati Medical College and Hospital, Guwahati, Assam, India.
[Ti] Título:Hyperkeratotic porokeratosis ptychotropica with satellite lesions: a rare presentation of an unusual variant of porokeratosis.
[So] Source:Acta Dermatovenerol Alp Pannonica Adriat;26(4):113-114, 2017 Dec.
[Is] ISSN:1581-2979
[Cp] País de publicação:Slovenia
[La] Idioma:eng
[Ab] Resumo:Since its description in 1995, porokeratosis ptychotropica (PP) has remained a less-recognized variant of porokeratosis (PK). The term porokeratosis ptychotropica was coined in reference to its characteristic of affecting body folds. It mimics many other dermatological diseases and is therefore often misdiagnosed. We report a patient with multiple hyperkeratotic, warty lesions across the buttocks that mimicked cutaneous tuberculosis (CTB), but histological examination confirmed the correct diagnosis of PP.
[Mh] Termos MeSH primário: Nádegas/patologia
Derme/patologia
Poroceratose/diagnóstico por imagem
Poroceratose/patologia
[Mh] Termos MeSH secundário: Adulto
Dermoscopia
Diagnóstico Diferencial
Feminino
Seres Humanos
Tuberculose Cutânea/diagnóstico
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180201
[Lr] Data última revisão:
180201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171222
[St] Status:MEDLINE


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[PMID]:29182510
[Au] Autor:Soe HJ; Khan AM; Manikam R; Samudi Raju C; Vanhoutte P; Sekaran SD
[Ad] Endereço:1​Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
[Ti] Título:High dengue virus load differentially modulates human microvascular endothelial barrier function during early infection.
[So] Source:J Gen Virol;98(12):2993-3007, 2017 Dec.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Plasma leakage is the main pathophysiological feature in severe dengue, resulting from altered vascular barrier function associated with an inappropriate immune response triggered upon infection. The present study investigated functional changes using an electric cell-substrate impedance sensing system in four (brain, dermal, pulmonary and retinal) human microvascular endothelial cell (MEC) lines infected with purified dengue virus, followed by assessment of cytokine profiles and the expression of inter-endothelial junctional proteins. Modelling of changes in electrical impedance suggests that vascular leakage in dengue-infected MECs is mostly due to the modulation of cell-to-cell interactions, while this loss of vascular barrier function observed in the infected MECs varied between cell lines and DENV serotypes. High levels of inflammatory cytokines (IL-6 and TNF-α), chemokines (CXCL1, CXCL5, CXCL11, CX3CL1, CCL2 and CCL20) and adhesion molecules (VCAM-1) were differentially produced in the four infected MECs. Further, the tight junctional protein, ZO-1, was down-regulated in both the DENV-1-infected brain and pulmonary MECs, while claudin-1, PECAM-1 and VE-cadherin were differentially expressed in these two MECs after infection. Non-purified virus stock was also studied to investigate the impact of virus stock purity on dengue-specific immune responses, and the results suggest that virus stock propagated through cell culture may include factors that mask or alter the DENV-specific immune responses of the MECs. The findings of the present study show that high DENV load differentially modulates human microvascular endothelial barrier function and disrupts the function of inter-endothelial junctional proteins during early infection with organ-specific cytokine production.
[Mh] Termos MeSH primário: Células Endoteliais/virologia
Endotélio Vascular/virologia
Interações Hospedeiro-Patógeno
Carga Viral/imunologia
[Mh] Termos MeSH secundário: Antígenos CD/genética
Antígenos CD/imunologia
Encéfalo/citologia
Encéfalo/imunologia
Encéfalo/virologia
Caderinas/genética
Caderinas/imunologia
Linhagem Celular
Quimiocina CCL2/genética
Quimiocina CCL2/imunologia
Quimiocina CCL20/genética
Quimiocina CCL20/imunologia
Quimiocina CX3CL1/genética
Quimiocina CX3CL1/imunologia
Quimiocinas CXC/genética
Quimiocinas CXC/imunologia
Claudina-1/genética
Claudina-1/imunologia
Vírus da Dengue/genética
Vírus da Dengue/crescimento & desenvolvimento
Vírus da Dengue/imunologia
Derme/citologia
Derme/imunologia
Derme/virologia
Impedância Elétrica
Células Endoteliais/citologia
Células Endoteliais/imunologia
Endotélio Vascular/citologia
Endotélio Vascular/imunologia
Regulação da Expressão Gênica
Seres Humanos
Interleucina-6/genética
Interleucina-6/imunologia
Pulmão/citologia
Pulmão/imunologia
Pulmão/virologia
Especificidade de Órgãos
Permeabilidade
Molécula-1 de Adesão Celular Endotelial de Plaquetas/genética
Molécula-1 de Adesão Celular Endotelial de Plaquetas/imunologia
Retina/citologia
Retina/imunologia
Retina/virologia
Transdução de Sinais
Fator de Necrose Tumoral alfa/genética
Fator de Necrose Tumoral alfa/imunologia
Molécula 1 de Adesão de Célula Vascular/genética
Molécula 1 de Adesão de Célula Vascular/imunologia
Internalização do Vírus
Proteína da Zônula de Oclusão-1/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, CD); 0 (CCL2 protein, human); 0 (CCL20 protein, human); 0 (CX3CL1 protein, human); 0 (Cadherins); 0 (Chemokine CCL2); 0 (Chemokine CCL20); 0 (Chemokine CX3CL1); 0 (Chemokines, CXC); 0 (Claudin-1); 0 (IL6 protein, human); 0 (Interleukin-6); 0 (Platelet Endothelial Cell Adhesion Molecule-1); 0 (TJP1 protein, human); 0 (Tumor Necrosis Factor-alpha); 0 (Vascular Cell Adhesion Molecule-1); 0 (Zonula Occludens-1 Protein); 0 (cadherin 5)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171213
[Lr] Data última revisão:
171213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171129
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000981


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[PMID]:28743501
[Au] Autor:Cui C; Lin H; Shi Y; Pan R
[Ad] Endereço:Department of Aesthetic Plastic Surgery and Laser Medicine, Beijing Anzhen Hospital, Capital Medical University, Anzhen Road #2, Chaoyang District, Beijing 100029, China. Electronic address: 15501008735@163.com.
[Ti] Título:Hypoxic postconditioning attenuates apoptosis via inactivation of adenosine A receptor through NDRG3-Raf-ERK pathway.
[So] Source:Biochem Biophys Res Commun;491(2):277-284, 2017 09 16.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: In recent years, many studies have demonstrated that endogenous adenosine induced by ischemia postconditioning reduces apoptosis in animal and cell models, but no study has clearly elucidated the effects of hypoxia postconditioning (HPC) in human dermal microvascular endothelial cells (HDMECs) of flaps, and the subtype of adenosine receptors involved remains unknown. In our study, we sought to identify the roles of adenosine A receptor, NDRG3 (N-myc downstream-regulated gene 3) and Raf-ERK pathway in the anti-apoptotic effects of hypoxia postconditioning. METHODS: Human dermal microvascular endothelial cells were put into a hypoxic incubator (94% N + 5% CO + 1% O ) for 8 h (hypoxia), and followed 24 h of normoxic culture with 95% air and 5% CO (reoxygenation). Hypoxia postconditioning model of HDMECs was achieved as follows: Before HDMECs were put into a normoxic incubator, HDMECs were treated by three cycles of 5 min of brief reoxygenation and 5 min of re-hypoxia. Opening level of mitochondrial permeability transition pore and change of mitochondrial membrane potential were detected with related Kit. The protein expressions of mitochondrion apoptosis, adenosine A receptor and NDRG3-Raf-ERK pathway were measured by western blot. RESULT: Hypoxia/reoxygenation (H/R) resulted in injury in HDMRCs as evidenced by an increase in apoptosis percentage, mitochondrial membrane permeability and an increase in expression of pro-apoptosis proteins (Bax, c-caspase-3 and cytochrom C), meanwhile, hypoxia/reoxygenation increased expression of A receptor, NDRG3, p-c-Raf, p-ERK, which was significantly attenuated by hypoxia postconditioning treatment. Moreover, Hypoxia/reoxygenation (H/R) resulted in a decrease in expression of anti-apoptotic protein (Bcl-2). However, the protective effect of hypoxia postconditioning treatment could be inhibited by adding CGS21680, a selective adenosine A receptor agonist (all P values < 0.05).
[Mh] Termos MeSH primário: Apoptose/efeitos dos fármacos
Células Endoteliais/efeitos dos fármacos
Pós-Condicionamento Isquêmico
Proteínas do Tecido Nervoso/genética
Oxigênio/farmacologia
Receptor A2A de Adenosina/genética
Quinases raf/genética
[Mh] Termos MeSH secundário: Adenosina/análogos & derivados
Adenosina/farmacologia
Agonistas do Receptor A2 de Adenosina/farmacologia
Apoptose/genética
Caspase 3/genética
Caspase 3/metabolismo
Hipóxia Celular
Derme/irrigação sanguínea
Derme/citologia
Derme/efeitos dos fármacos
Células Endoteliais/citologia
Células Endoteliais/metabolismo
Regulação da Expressão Gênica
Seres Humanos
Sistema de Sinalização das MAP Quinases/efeitos dos fármacos
Potencial da Membrana Mitocondrial
Mitocôndrias/efeitos dos fármacos
Mitocôndrias/metabolismo
Proteínas de Transporte da Membrana Mitocondrial/genética
Proteínas de Transporte da Membrana Mitocondrial/metabolismo
Modelos Biológicos
Proteínas do Tecido Nervoso/metabolismo
Fenetilaminas/farmacologia
Cultura Primária de Células
Proteínas Proto-Oncogênicas c-bcl-2/genética
Proteínas Proto-Oncogênicas c-bcl-2/metabolismo
Receptor A2A de Adenosina/metabolismo
Retalhos Cirúrgicos/irrigação sanguínea
Proteína X Associada a bcl-2/genética
Proteína X Associada a bcl-2/metabolismo
Quinases raf/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Adenosine A2 Receptor Agonists); 0 (BAX protein, human); 0 (BCL2 protein, human); 0 (Mitochondrial Membrane Transport Proteins); 0 (NDRG3 protein, human); 0 (Nerve Tissue Proteins); 0 (Phenethylamines); 0 (Proto-Oncogene Proteins c-bcl-2); 0 (Receptor, Adenosine A2A); 0 (bcl-2-Associated X Protein); 0 (mitochondrial permeability transition pore); 120225-54-9 (2-(4-(2-carboxyethyl)phenethylamino)-5'-N-ethylcarboxamidoadenosine); EC 2.7.11.1 (raf Kinases); EC 3.4.22.- (CASP3 protein, human); EC 3.4.22.- (Caspase 3); K72T3FS567 (Adenosine); S88TT14065 (Oxygen)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171129
[Lr] Data última revisão:
171129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE


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[PMID]:28590053
[Au] Autor:Khan TK; Wender PA; Alkon DL
[Ad] Endereço:Center for Neurodegenerative Diseases, Blanchette Rockefeller Neurosciences Institute at West Virginia University, Morgantown, West Virginia.
[Ti] Título:Bryostatin and its synthetic analog, picolog rescue dermal fibroblasts from prolonged stress and contribute to survival and rejuvenation of human skin equivalents.
[So] Source:J Cell Physiol;233(2):1523-1534, 2018 Feb.
[Is] ISSN:1097-4652
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Skin health is associated with the day-to-day activity of fibroblasts. The primary function of fibroblasts is to synthesize structural proteins, such as collagen, extracellular matrix proteins, and other proteins that support the structural integrity of the skin and are associated with younger, firmer, and more elastic skin that is better able to resist and recover from injury. At sub-nanomolar concentrations (0.03-0.3 nM), bryostatin-1 and its synthetic analog, picolog (0.1-10 nM) sustained the survival and activation of human dermal fibroblasts cultured under the stressful condition of prolonged serum deprivation. Bryostatin-1 treatment stabilized human skin equivalents (HSEs), a bioengineered combination of primary human skin cells (keratinocytes and dermal fibroblasts) on an extracellular matrix composed of mainly collagen. Fibroblasts activated by bryostatin-1 protected the structural integrity of HSEs. Bryostatin-1 and picolog prolonged activation of Erk in fibroblasts to promote cell survival. Chronic stress promotes the progression of apoptosis. Dermal fibroblasts constitutively express all components of Fas associated apoptosis, including caspase-8, an initiator enzyme of apoptosis. Prolong bryostatin-1 treatment reduced apoptosis by decreasing caspase-8 and protected dermal fibroblasts. Our data suggest that bryostatin-1 and picolog could be useful in anti-aging skincare, and could have applications in tissue engineering and regenerative medicine.
[Mh] Termos MeSH primário: Briostatinas/farmacologia
Derme/efeitos dos fármacos
Fibroblastos/efeitos dos fármacos
Rejuvenescimento
Estresse Fisiológico
Engenharia Tecidual/métodos
[Mh] Termos MeSH secundário: Adulto
Idoso
Apoptose/efeitos dos fármacos
Proteínas Reguladoras de Apoptose/metabolismo
Briostatinas/síntese química
Linhagem Celular
Sobrevivência Celular/efeitos dos fármacos
Técnicas de Cocultura
Colágeno/metabolismo
Meios de Cultura Livres de Soro/metabolismo
Derme/metabolismo
Derme/patologia
Relação Dose-Resposta a Droga
Ativação Enzimática
MAP Quinases Reguladas por Sinal Extracelular/metabolismo
Feminino
Fibroblastos/metabolismo
Fibroblastos/patologia
Seres Humanos
Queratinócitos/metabolismo
Masculino
Meia-Idade
Transdução de Sinais/efeitos dos fármacos
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Apoptosis Regulatory Proteins); 0 (Bryostatins); 0 (Culture Media, Serum-Free); 0 (picolog); 9007-34-5 (Collagen); EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170608
[St] Status:MEDLINE
[do] DOI:10.1002/jcp.26043


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[PMID]:29055410
[Au] Autor:Lei M; Yang L; Chuong CM
[Ad] Endereço:"111" Project Laboratory of Biomechanics and Tissue Repair and Key Laboratory of Biorheological Science and Technology of Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, China; Department of Pathology, Keck School of Medicine, University of Southern California, Los Angeles, California, USA; Integrative Stem Cell Center, China Medical University Hospital, China Medical University, Taichung, Taiwan; Institute of New Drug Development, College of Biopharmaceutical and Food Sciences, China Medical University, Taichung, Taiwan.
[Ti] Título:Getting to the Core of the Dermal Papilla.
[So] Source:J Invest Dermatol;137(11):2250-2253, 2017 Nov.
[Is] ISSN:1523-1747
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Through coordinated and reciprocal interactions between the epithelium and mesenchyme, diverse integumentary organs form and undergo cyclic renewal. The hair follicle has become the main model to understand this extraordinary regenerative behavior. At the core is the dermal papilla, the organizing center, and the epithelial stem cells that respond to dermal papilla signaling. Two recent papers by Telerman et al. and Yang et al. unravel new molecular landscapes within the dermal papilla.
[Mh] Termos MeSH primário: Epitélio/fisiologia
Folículo Piloso/fisiologia
Mesoderma/fisiologia
Regeneração/genética
Via de Sinalização Wnt/genética
[Mh] Termos MeSH secundário: Células Cultivadas
Derme/fisiologia
Células Germinativas/metabolismo
Células Germinativas/fisiologia
Cabelo/crescimento & desenvolvimento
Seres Humanos
Sensibilidade e Especificidade
Transdução de Sinais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171023
[St] Status:MEDLINE


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[PMID]:28954103
[Au] Autor:Almeida HL; Almeida MG; Jorge VM; Abreu LB
[Ad] Endereço:Department of Dermatology, Universidade Federal de Pelotas (UFPel) - Pelotas (RS), Brazil.
[Ti] Título:Ultrastructural aspects of pseudoxanthoma elasticum.
[So] Source:An Bras Dermatol;92(4):527-530, 2017 Jul-Aug.
[Is] ISSN:1806-4841
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:We report the ultrastructural findings in a case of a 72-year-old black woman with confluent yellowish papules in the cervical region. She had no comorbidities. Ophthalmological examination, electrocardiogram, and echocardiogram were normal. Hematoxylin-eosin staining of the affected skin showed strong alterations in the mid-dermis with irregular clumps of eosinophilic material and loss of the normal parallel arrangement of collagen bundles. Orcein staining revealed that the elastic fibers lost their normal linear configuration, showing clump fragmentation, sometimes forming square structures. Transmission electron microscopy showed aberrant elastic fibers with an irregular outline and heterogenic inner structures. We also observed small elastic fibers. Collagen fibers showed a normal structure with irregular distribution. Scanning electron microscopy revealed important disorganization of collagen fibers and small stone-like deposits measuring around 5 µm associated with bigger structures ranging from 10-16 µm. Higher magnification revealed that these small stone-like structures were sometimes polyhedral-shaped or squared.
[Mh] Termos MeSH primário: Derme/ultraestrutura
Tecido Elástico/ultraestrutura
Pseudoxantoma Elástico/patologia
[Mh] Termos MeSH secundário: Idoso
Colágeno/ultraestrutura
Feminino
Seres Humanos
Microscopia Eletrônica de Varredura
Microscopia Eletrônica de Transmissão
Pele/patologia
Coluna Vertebral
Coloração e Rotulagem
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
9007-34-5 (Collagen)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170928
[St] Status:MEDLINE



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