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[PMID]: 29437642 [Au] Autor: Davies JM; Thien F; Hew M [Ad] Endereço: Office of Research, Institute of Health and Biomedical Innovation, Queensland University of Technology, Queensland, Australia, and Metro North Hospital and Health Service, Brisbane, Queensland, Australia. [Ti] Título: Thunderstorm asthma: controlling (deadly) grass pollen allergy. [So] Source: BMJ;360:k432, 2018 02 06. [Is] ISSN: 1756-1833 [Cp] País de publicação: England [La] Idioma: eng [Mh] Termos MeSH primário: Asma Rinite Alérgica Sazonal [Mh] Termos MeSH secundário: Alérgenos Seres Humanos Poaceae/imunologia Pólen/imunologia [Pt] Tipo de publicação: LETTER; RESEARCH SUPPORT, NON-U.S. GOV'T; COMMENT [Nm] Nome de substância: 0 (Allergens) [Em] Mês de entrada: 1803 [Cu] Atualização por classe: 180305 [Lr] Data última revisão: 180305 [Sb] Subgrupo de revista: AIM; IM [Da] Data de entrada para processamento: 180214 [St] Status: MEDLINE [do] DOI: 10.1136/bmj.k432

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[PMID]: 29382326 [Au] Autor: Chen X; Wu RZ; Zhu YQ; Ren ZM; Tong YL; Yang F; Dai GH [Ad] Endereço: Institute of Basic Medicine, Zhejiang Academy of Traditional Chinese Medicine, No. 132, Tianmushan Road, Xihu District, Hangzhou, Zhejiang, China. [Ti] Título: Study on the inhibition of Mfn1 by plant-derived miR5338 mediating the treatment of BPH with rape bee pollen. [So] Source: BMC Complement Altern Med;18(1):38, 2018 Jan 30. [Is] ISSN: 1472-6882 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: BACKGROUND: Recent studies have found that plant derived microRNA can cross-kingdom regulate the expression of genes in humans and other mammals, thereby resisting diseases. Can exogenous miRNAs cross the blood-prostate barrier and entry prostate then participate in prostate disease treatment? METHODS: Using HiSeq sequencing and RT-qPCR technology, we detected plant miRNAs that enriched in the prostates of rats among the normal group, BPH model group and rape bee pollen group. To forecast the functions of these miRNAs, the psRobot software and TargetFinder software were used to predict their candidate target genes in rat genome. The qRT-PCR technology was used to validate the expression of candidate target genes. RESULTS: Plant miR5338 was enriched in the posterior lobes of prostate gland of rats fed with rape bee pollen, which was accompanied by the improvement of BPH. Among the predicted target genes of miR5338, Mfn1 was significantly lower in posterior lobes of prostates of rats in the rape bee pollen group than control groups. Further experiments suggested that Mfn1 was highly related to BPH. CONCLUSIONS: These results suggesting that plant-derived miR5338 may involve in treatment of rat BPH through inhibiting Mfn1 in prostate. These results will provide more evidence for plant miRNAs cross-kingdom regulation of animal gene, and will provide preliminary theoretical and experimental basis for development of rape bee pollen into innovative health care product or medicine for the treatment of BPH. [Mh] Termos MeSH primário: Proteínas de Membrana/antagonistas & inibidores MicroRNAs/farmacologia Proteínas Mitocondriais/antagonistas & inibidores Pólen Próstata/efeitos dos fármacos Hiperplasia Prostática/metabolismo RNA de Plantas/farmacologia [Mh] Termos MeSH secundário: Animais Abelhas Peso Corporal/efeitos dos fármacos Masculino Proteínas de Membrana/metabolismo Proteínas Mitocondriais/metabolismo Tamanho do Órgão/efeitos dos fármacos RNA de Plantas/farmacocinética Ratos [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Membrane Proteins); 0 (MicroRNAs); 0 (Mitochondrial Proteins); 0 (RNA, Plant); 0 (mitofusin 1 protein, rat) [Em] Mês de entrada: 1803 [Cu] Atualização por classe: 180301 [Lr] Data última revisão: 180301 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180201 [St] Status: MEDLINE [do] DOI: 10.1186/s12906-018-2107-y

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[PMID]: 29324841 [Au] Autor: Corby-Harris V; Snyder L; Meador C; Ayotte T [Ad] Endereço: Carl Hayden Bee Research Center, USDA-ARS, Tucson, Arizona, United States of America. [Ti] Título: Honey bee (Apis mellifera) nurses do not consume pollens based on their nutritional quality. [So] Source: PLoS One;13(1):e0191050, 2018. [Is] ISSN: 1932-6203 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Honey bee workers (Apis mellifera) consume a variety of pollens to meet the majority of their requirements for protein and lipids. Recent work indicates that honey bees prefer diets that reflect the proper ratio of nutrients necessary for optimal survival and homeostasis. This idea relies on the precept that honey bees evaluate the nutritional composition of the foods provided to them. While this has been shown in bumble bees, the data for honey bees are mixed. Further, there is controversy as to whether foragers can evaluate the nutritional value of pollens, especially if they do not consume it. Here, we focused on nurse workers, who eat most of the pollen coming into the hive. We tested the hypothesis that nurses prefer diets with higher nutritional value. We first determined the nutritional profile, number of plant taxa (richness), and degree of hypopharyngeal gland (HG) growth conferred by three honey bee collected pollens. We then presented nurses with these same three pollens in paired choice assays and measured consumption. To further test whether nutrition influenced preference, we also presented bees with natural pollens supplemented with protein or lipids and liquid diets with protein and lipid ratios equal to the natural pollens. Different pollens conferred different degrees of HG growth, but despite these differences, nurse bees did not always prefer the most nutritious pollens. Adding protein and/or lipids to less desirable pollens minimally increased pollen attractiveness, and nurses did not exhibit a strong preference for any of the three liquid diets. We conclude that different pollens provide different nutritional benefits, but that nurses either cannot or do not assess pollen nutritional value. This implies that the nurses may not be able to communicate information about pollen quality to the foragers, who regulate the pollens coming into the hive. [Mh] Termos MeSH primário: Abelhas/fisiologia Valor Nutritivo Pólen [Mh] Termos MeSH secundário: Animais [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180223 [Lr] Data última revisão: 180223 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180112 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pone.0191050

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[PMID]: 29222859 [Au] Autor: Tyliszczak B; Drabczyk A; Kudlacik-Kramarczyk S; Grabowska B; Kedzierska M [Ad] Endereço: Department of Chemistry and Technology of Polymers, Cracow University of Technology, Kraków, Poland. [Ti] Título: Physicochemical properties and cytotoxicity of hydrogels based on Beetosan® containing sage and bee pollen. [So] Source: Acta Biochim Pol;64(4):709-712, 2017. [Is] ISSN: 1734-154X [Cp] País de publicação: Poland [La] Idioma: eng [Ab] Resumo: Currently, increasing attention is being paid to issues related to environmental protection, waste management, as well as to the development of polymers with useful properties. The research presented here involved preparation of hydrogels based on Beetosan® - a chitosan derived from the multi-stage processing of dead bees. Moreover, hydrogels were additionally modified with natural substances - i.e. bee pollen and extract of Salvia officinalis (sage) that are well known for the presence of many compounds with beneficial properties from a medical point of view. Materials have been first obtained by photopolymerization. Then, their surface morphology, wettability and cytotoxicity to selected cell lines have been determined. It can be stated that such combination of Beetosan® hydrogel matrix and the mentioned additives resulted in a preparation of polymers characterized by negative impact on cancer cells. Impact of hydrogels with sage is slightly more intense due to the presence of substances such as ursalic or rosmaric acid that are characterized to have anticancer activity. Such negative impact has not been observed in case of studies using fibroblasts. Furthermore, addition of natural substances into hydrogels resulted in a more homogeneous surface and in the decrease of wettability angle of the tested polymers. It can be concluded that the use of natural-derived reagents and synthesis of polymers using these reagents (as a result of environmentally friendly photopolymerization) yields materials with interesting properties for medical purposes, with particular emphasis on antitumor activity, and without significant negative impact on fibroblasts. [Mh] Termos MeSH primário: Hidrogéis/química Hidrogéis/toxicidade Pólen/química [Mh] Termos MeSH secundário: Animais Abelhas Quitosana/química Seres Humanos Células Jurkat Microscopia Eletrônica de Varredura Salvia officinalis Testes de Toxicidade [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Hydrogels); 9012-76-4 (Chitosan) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180223 [Lr] Data última revisão: 180223 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171210 [St] Status: MEDLINE [do] DOI: 10.18388/abp.2017_2319

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[PMID]: 27778095 [Au] Autor: He S; Mou Z; Peng L; Chen J [Ad] Endereço: Department of Otorhinolaryngology, Shanghai Children's Medical Center, affiliated with Shanghai Jiaotong University School of Medicine, Shanghai, 200127, China. [Ti] Título: Impacts of meteorological and environmental factors on allergic rhinitis in children. [So] Source: Int J Biometeorol;61(5):797-806, 2017 May. [Is] ISSN: 1432-1254 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Meteorological and environmental factors influence the pathogenesis of allergic rhinitis (AR). An understanding of the risk factors will facilitate the development of diagnostic and preventative tools for AR children and improve their quality of life. However, research on the impact of these factors on subjective symptoms in AR children remains scarce. This study explored the relationships between subjective symptoms in pollen and dust mite positive AR children, and meteorological and environmental factors. Using a linear mixed effect model, we analyzed the correlations between monthly data on the subjective symptoms of 351 AR children (from the Shanghai Children's Medical Center) and meteorological and environmental factors during 2013. The monthly meteorological and environmental data were provided by the Shanghai Meteorological Service and Shanghai Environmental Protection Bureau. Temperature and humidity were negatively correlated with the subjective symptom score, with a 0.04 point increase observed for every 1 °C decrease in temperature (P < 0.0001) or 10 % decline in humidity (P = 0.0412). The particulate matter (PM) 10 and PM2.5 concentrations were positively correlated with the subjective symptom score, with a 10 µg/m increase in PM10 and PM2.5 yielding a 0.02 (P = 0.0235) and 0.03 (P = 0.0281) increase in the subjective symptom score, respectively. In conclusion, meteorological and environmental factors were correlated with subjective symptoms in AR children. Low temperatures, lower humidity, and high PM10 and PM2.5 concentrations aggravated subjective symptoms in AR children. [Mh] Termos MeSH primário: Rinite Alérgica/etiologia [Mh] Termos MeSH secundário: Adolescente Poluentes Atmosféricos Alérgenos/imunologia Animais Criança Pré-Escolar China/epidemiologia Feminino Seres Humanos Umidade Modelos Lineares Masculino Material Particulado Pólen/imunologia Pyroglyphidae/imunologia Rinite Alérgica/epidemiologia Fatores de Risco Temperatura Ambiente [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Air Pollutants); 0 (Allergens); 0 (Particulate Matter) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180222 [Lr] Data última revisão: 180222 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161026 [St] Status: MEDLINE [do] DOI: 10.1007/s00484-016-1257-1

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[PMID]: 29346453 [Au] Autor: Flo V; Bosch J; Arnan X; Primante C; Martín González AM; Barril-Graells H; Rodrigo A [Ad] Endereço: CREAF, Center for Ecological Research and Forestry Applications, Campus UAB, Bellaterra, Spain. [Ti] Título: Yearly fluctuations of flower landscape in a Mediterranean scrubland: Consequences for floral resource availability. [So] Source: PLoS One;13(1):e0191268, 2018. [Is] ISSN: 1932-6203 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Species flower production and flowering phenology vary from year to year due to extrinsic factors. Inter-annual variability in flowering patterns may have important consequences for attractiveness to pollinators, and ultimately, plant reproductive output. To understand the consequences of flowering pattern variability, a community approach is necessary because pollinator flower choice is highly dependent on flower context. Our objectives were: 1) To quantify yearly variability in flower density and phenology; 2) To evaluate whether changes in flowering patterns result in significant changes in pollen/nectar composition. We monitored weekly flowering patterns in a Mediterranean scrubland community (23 species) over 8 years. Floral resource availability was estimated based on field measures of pollen and nectar production per flower. We analysed inter-annual variation in flowering phenology (duration and date of peak bloom) and flower production, and inter-annual and monthly variability in flower, pollen and nectar species composition. We also investigated potential phylogenetic effects on inter-annual variability of flowering patterns. We found dramatic variation in yearly flower production both at the species and community levels. There was also substantial variation in flowering phenology. Importantly, yearly fluctuations were far from synchronous across species, and resulted in significant changes in floral resources availability and composition at the community level. Changes were especially pronounced late in the season, at a time when flowers are scarce and pollinator visitation rates are particularly high. We discuss the consequences of our findings for pollinator visitation and plant reproductive success in the current scenario of climate change. [Mh] Termos MeSH primário: Flores [Mh] Termos MeSH secundário: Animais Ecossistema Flores/crescimento & desenvolvimento Flores/fisiologia Região do Mediterrâneo Filogenia Néctar de Plantas/biossíntese Pólen/crescimento & desenvolvimento Polinização Reprodução Estações do Ano Espanha Especificidade da Espécie [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Plant Nectar) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180220 [Lr] Data última revisão: 180220 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180119 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pone.0191268

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[PMID]: 29313353 [Au] Autor: Zhou W; Yan Y; Mi J; Zhang H; Lu L; Luo Q; Li X; Zeng X; Cao Y [Ad] Endereço: College of Food Science and Technology, Nanjing Agricultural University , Nanjing 210095, People's Republic of China. [Ti] Título: Simulated Digestion and Fermentation in Vitro by Human Gut Microbiota of Polysaccharides from Bee Collected Pollen of Chinese Wolfberry. [So] Source: J Agric Food Chem;66(4):898-907, 2018 Jan 31. [Is] ISSN: 1520-5118 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Digestion and fermentation in vitro of polysaccharides from bee collected pollen of Chinese wolfberry (WBPPS) were investigated in the present study. It was found that WBPPS mainly consisted of mannose, ribose, rhamnose, galacturonic acid, glucose, galactose, xylose, and arabinose in a molar ratio of 0.38:0.09:0.17:0.64:0.22:0.67:0.08:1.03, respectively. WBPPS was not affected by human saliva. The fraction A (molecular weight 1340 kDa) of WBPPS was not broken down in simulated gastric and small intestinal juices, while the small fraction B (molecular weight 523 kDa) of WBPPS was degraded. Moreover, fermentation in vitro revealed that WBPPS could significantly enhance the production of short-chain fatty acids and modulate gut microbiota composition via increasing the relative abundances of genera Prevotella, Dialister, Megamonas, Faecalibacterium, and Alloprevotella and decreasing the numbers of genera Bacteroides, Clostridium XlVa, Parabacteroides, Escherichia/Shigella, Phascolarctobacterium, Parasutterella, Clostridium sensu stricto, and Fusobacterium. [Mh] Termos MeSH primário: Abelhas Digestão Fermentação Microbioma Gastrointestinal/fisiologia Lycium Pólen/química Polissacarídeos/metabolismo [Mh] Termos MeSH secundário: Animais Bactérias/isolamento & purificação Bactérias/metabolismo China Ácidos Graxos Voláteis/biossíntese Fezes/microbiologia Seres Humanos Saliva/enzimologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Fatty Acids, Volatile); 0 (Polysaccharides) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180212 [Lr] Data última revisão: 180212 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180110 [St] Status: MEDLINE [do] DOI: 10.1021/acs.jafc.7b05546

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[PMID]: 29401893 [Au] Autor: Kim Y; Warren S; Favero F; Stone J; Clegg J; Neil M; Paterson C; Knight J; French P; Dunsby C [Ti] Título: Semi-random multicore fibre design for adaptive multiphoton endoscopy. [So] Source: Opt Express;26(3):3661-3673, 2018 Feb 05. [Is] ISSN: 1094-4087 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: This paper reports the development, modelling and application of a semi-random multicore fibre (MCF) design for adaptive multiphoton endoscopy. The MCF was constructed from 55 sub-units, each comprising 7 single mode cores, in a hexagonally close-packed lattice where each sub-unit had a random angular orientation. The resulting fibre had 385 single mode cores and was double-clad for proximal detection of multiphoton excited fluorescence. The random orientation of each sub-unit in the fibre reduces the symmetry of the positions of the cores in the MCF, reducing the intensity of higher diffracted orders away from the central focal spot formed at the distal tip of the fibre and increasing the maximum size of object that can be imaged. The performance of the MCF was demonstrated by imaging fluorescently labelled beads with both distal and proximal fluorescence detection and pollen grains with distal fluorescence detection. We estimate that the number of independent resolution elements in the final image - measured as the half-maximum area of the two-photon point spread function divided by the area imaged - to be ~3200. [Mh] Termos MeSH primário: Endoscópios Endoscopia/instrumentação Microscopia de Fluorescência por Excitação Multifotônica Fibras Ópticas [Mh] Termos MeSH secundário: Desenho de Equipamento Microesferas Pólen [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180208 [Lr] Data última revisão: 180208 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180207 [St] Status: MEDLINE [do] DOI: 10.1364/OE.26.003661

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[PMID]: 29401892 [Au] Autor: Swanson BE; Huffman JA [Ti] Título: Development and characterization of an inexpensive single-particle fluorescence spectrometer for bioaerosol monitoring. [So] Source: Opt Express;26(3):3646-3660, 2018 Feb 05. [Is] ISSN: 1094-4087 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Laser-induced fluorescence (LIF) techniques to analyze atmospheric aerosols are commonly applied for research and human exposure monitoring, but are very expensive or offer poor spectral resolution. Here, we discuss how a recently proposed instrument can acquire resolved fluorescence spectra from many individual particles in a single camera image using four excitation wavelengths matched with common biological fluorophores for particle discrimination at lower cost. We discuss emission intensity calibration and demonstrate spectral differentiation among four species of pollen. These data provide context for how the instrument could be developed for pollen and mold-spore detection or for use by citizen scientists. [Mh] Termos MeSH primário: Fungos Pólen Espectrometria de Fluorescência/instrumentação [Mh] Termos MeSH secundário: Calibragem Seres Humanos Lasers [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180208 [Lr] Data última revisão: 180208 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180207 [St] Status: MEDLINE [do] DOI: 10.1364/OE.26.003646

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[PMID]: 29217674 [Au] Autor: Losada JM; Hormaza JI; Lora J [Ad] Endereço: Arnold Arboretum of Harvard University, 1300 Centre Street, Boston, Massachusetts 02131 USA. [Ti] Título: Pollen-pistil interaction in pawpaw ( ), the northernmost species of the mainly tropical family Annonaceae. [So] Source: Am J Bot;104(12):1891-1903, 2017 Dec. [Is] ISSN: 1537-2197 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: PREMISE OF THE STUDY: The pawpaw, , is an underutilized fruit crop native to North America that belongs to the mainly tropical, early-divergent family Annonaceae. is the only genus within the Annonaceae with species adapted to cold climates. A thorough analysis of its reproductive biology, specifically pollen-pistil interaction during the progamic phase, is essential to understand both its adaptation to cold climates and how to optimize its fertilization and fruit set. METHODS: We characterized pollen-pistil interaction in , including the floral cycle and anatomy, stigmatic receptivity, and the pollen tube pathway. We used a combination of histological, cytological, and immunolocalization approaches. KEY RESULTS: has a gynoecium formed by plicate carpels with a short stylar canal. Unicellular papillae form a continuous tissue covered by a copious secretion from the stigma to the ovary, which is most prominent on the stigma surface where it forms an extragynoecial compitum. Compared to the stigmas of other species in the Annonaceae, the stigmas of show a long stigmatic receptivity associated with a long flowering cycle. Stigmatic receptivity is concomitant with the secretion of cell-wall-related arabinogalactan proteins (AGPs). CONCLUSIONS: A long female phase with a long period of stigmatic receptivity is unusual among protogynous flowers of the magnoliid clade, suggesting a derived condition of within the Annonaceae. This phase further correlates with the presence of cell-wall-related arabinogalactan proteins in the secretion, which may indicate the conservation of these glycoproteins during stigmatic receptivity and pollen tube growth in angiosperms. [Mh] Termos MeSH primário: Asimina/fisiologia Flores Pólen Polinização/fisiologia [Mh] Termos MeSH secundário: Demografia [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180207 [Lr] Data última revisão: 180207 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171209 [St] Status: MEDLINE [do] DOI: 10.3732/ajb.1700319

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