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Pesquisa : A18.024.500.750.333 [Categoria DeCS]
Referências encontradas : 1685 [refinar]
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  1 / 1685 MEDLINE  
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[PMID]:28465411
[Au] Autor:Xiong Q; Ma B; Lu X; Huang YH; He SJ; Yang C; Yin CC; Zhao H; Zhou Y; Zhang WK; Wang WS; Li ZK; Chen SY; Zhang JS
[Ad] Endereço:State Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
[Ti] Título:Ethylene-Inhibited Jasmonic Acid Biosynthesis Promotes Mesocotyl/Coleoptile Elongation of Etiolated Rice Seedlings.
[So] Source:Plant Cell;29(5):1053-1072, 2017 May.
[Is] ISSN:1532-298X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Elongation of the mesocotyl and coleoptile facilitates the emergence of rice ( ) seedlings from soil and is affected by various genetic and environment factors. The regulatory mechanism underlying this process remains largely unclear. Here, we examined the regulation of mesocotyl and coleoptile growth by characterizing a ( ) mutant that exhibits a longer mesocotyl and longer coleoptile than its original variety of rice. was identified through map-based cloning and encodes a PLA -type phospholipase that localizes in chloroplasts. GY1 functions at the initial step of jasmonic acid (JA) biosynthesis to repress mesocotyl and coleoptile elongation in etiolated rice seedlings. Ethylene inhibits the expression of and other genes in the JA biosynthesis pathway to reduce JA levels and enhance mesocotyl and coleoptile growth by promoting cell elongation. Genetically, acts downstream of the OsEIN2-mediated ethylene signaling pathway to regulate mesocotyl/coleoptile growth. Through analysis of the resequencing data from 3000 rice accessions, we identified a single natural variation of the gene, , which contributes to mesocotyl elongation in rice varieties. Our study reveals novel insights into the regulatory mechanism of mesocotyl/coleoptile elongation and should have practical applications in rice breeding programs.
[Mh] Termos MeSH primário: Ciclopentanos/metabolismo
Etilenos/farmacologia
Oryza/efeitos dos fármacos
Oryza/metabolismo
Oxilipinas/metabolismo
Plântulas/efeitos dos fármacos
Plântulas/metabolismo
[Mh] Termos MeSH secundário: Cotilédone/efeitos dos fármacos
Cotilédone/genética
Cotilédone/metabolismo
Regulação da Expressão Gênica de Plantas/efeitos dos fármacos
Regulação da Expressão Gênica de Plantas/genética
Oryza/genética
Proteínas de Plantas/genética
Proteínas de Plantas/metabolismo
Plântulas/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cyclopentanes); 0 (Ethylenes); 0 (Oxylipins); 0 (Plant Proteins); 6RI5N05OWW (jasmonic acid); 91GW059KN7 (ethylene)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171212
[Lr] Data última revisão:
171212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1105/tpc.16.00981


  2 / 1685 MEDLINE  
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[PMID]:28981522
[Au] Autor:Karmous I; Trevisan R; El Ferjani E; Chaoui A; Sheehan D
[Ad] Endereço:Plant Toxicology and Molecular Biology of Microorganisms, Faculty of Sciences of Bizerta, Zarzouna, Tunisia.
[Ti] Título:Redox biology response in germinating Phaseolus vulgaris seeds exposed to copper: Evidence for differential redox buffering in seedlings and cotyledon.
[So] Source:PLoS One;12(10):e0184396, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In agriculture, heavy metal contamination of soil interferes with processes associated with plant growth, development and productivity. Here, we describe oxidative and redox changes, and deleterious injury within cotyledons and seedlings caused by exposure of germinating (Phaseolus vulgaris L. var. soisson nain hâtif) seeds to copper (Cu). Cu induced a marked delay in seedling growth, and was associated with biochemical disturbances in terms of intracellular oxidative status, redox regulation and energy metabolism. In response to these alterations, modulation of activities of antioxidant proteins (thioredoxin and glutathione reductase, peroxiredoxin) occurred, thus preventing oxidative damage. In addition, oxidative modification of proteins was detected in both cotyledons and seedlings by one- and two-dimensional electrophoresis. These modified proteins may play roles in redox buffering. The changes in activities of redox proteins underline their fundamental roles in controlling redox homeostasis. However, observed differential redox responses in cotyledon and seedling tissues showed a major capacity of the seedlings' redox systems to protect the reduced status of protein thiols, thus suggesting quantitatively greater antioxidant protection of proteins in seedlings compared to cotyledon. To our knowledge, this is the first comprehensive redox biology investigation of the effect of Cu on seed germination.
[Mh] Termos MeSH primário: Cobre/farmacologia
Cotilédone/efeitos dos fármacos
Germinação/efeitos dos fármacos
Phaseolus/efeitos dos fármacos
Plântulas/efeitos dos fármacos
[Mh] Termos MeSH secundário: Cotilédone/metabolismo
Germinação/fisiologia
Oxirredução
Phaseolus/metabolismo
Plântulas/metabolismo
Solo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Soil); 789U1901C5 (Copper)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171006
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184396


  3 / 1685 MEDLINE  
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[PMID]:28961247
[Au] Autor:Ke X; Zou W; Ren Y; Wang Z; Li J; Wu X; Zhao J
[Ad] Endereço:State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, China.
[Ti] Título:Functional divergence of chloroplast Cpn60α subunits during Arabidopsis embryo development.
[So] Source:PLoS Genet;13(9):e1007036, 2017 Sep.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Chaperonins are a class of molecular chaperones that assist in the folding and assembly of a wide range of substrates. In plants, chloroplast chaperonins are composed of two different types of subunits, Cpn60α and Cpn60ß, and duplication of Cpn60α and Cpn60ß genes occurs in a high proportion of plants. However, the importance of multiple Cpn60α and Cpn60ß genes in plants is poorly understood. In this study, we found that loss-of-function of CPNA2 (AtCpn60α2), a gene encoding the minor Cpn60α subunit in Arabidopsis thaliana, resulted in arrested embryo development at the globular stage, whereas the other AtCpn60α gene encoding the dominant Cpn60α subunit, CPNA1 (AtCpn60α1), mainly affected embryonic cotyledon development at the torpedo stage and thereafter. Further studies demonstrated that CPNA2 can form a functional chaperonin with CPNB2 (AtCpn60ß2) and CPNB3 (AtCpn60ß3), while the functional partners of CPNA1 are CPNB1 (AtCpn60ß1) and CPNB2. We also revealed that the functional chaperonin containing CPNA2 could assist the folding of a specific substrate, KASI (ß-ketoacyl-[acyl carrier protein] synthase I), and that the KASI protein level was remarkably reduced due to loss-of-function of CPNA2. Furthermore, the reduction in the KASI protein level was shown to be the possible cause for the arrest of cpna2 embryos. Our findings indicate that the two Cpn60α subunits in Arabidopsis play different roles during embryo development through forming distinct chaperonins with specific AtCpn60ß to assist the folding of particular substrates, thus providing novel insights into functional divergence of Cpn60α subunits in plants.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/metabolismo
Arabidopsis/genética
Chaperoninas/metabolismo
Regulação da Expressão Gênica de Plantas
Genes de Plantas
[Mh] Termos MeSH secundário: 3-Oxoacil-(Proteína de Transporte de Acila) Sintase/genética
3-Oxoacil-(Proteína de Transporte de Acila) Sintase/metabolismo
Proteína de Transporte de Acila/genética
Proteína de Transporte de Acila/metabolismo
Sequência de Aminoácidos
Arabidopsis/embriologia
Arabidopsis/crescimento & desenvolvimento
Proteínas de Arabidopsis/genética
Chaperoninas/genética
Cloroplastos/genética
Cloroplastos/metabolismo
Clonagem Molecular
Cotilédone/embriologia
Cotilédone/genética
Duplicação Gênica
Conformação Proteica
Plântulas/embriologia
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acyl Carrier Protein); 0 (Arabidopsis Proteins); EC 2.3.1.41 (3-Oxoacyl-(Acyl-Carrier-Protein) Synthase); EC 3.6.1.- (Chaperonins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170930
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1007036


  4 / 1685 MEDLINE  
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[PMID]:28715427
[Au] Autor:Dou L; Jia X; Wei H; Fan S; Wang H; Guo Y; Duan S; Pang C; Yu S
[Ad] Endereço:State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang, Henan, P. R. China.
[Ti] Título:Global analysis of DNA methylation in young (J1) and senescent (J2) Gossypium hirsutum L. cotyledons by MeDIP-Seq.
[So] Source:PLoS One;12(7):e0179141, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:DNA methylation is an important epigenetic modification regulating gene expression, genomic imprinting, transposon silencing and chromatin structure in plants and plays an important role in leaf senescence. However, the DNA methylation pattern during Gossypium hirsutum L. cotyledon senescence is poorly understood. In this study, global DNA methylation patterns were compared between two cotyledon development stages, young (J1) and senescence (J2), using methylated DNA immunoprecipitation (MeDIP-Seq). Methylated cytosine occurred mostly in repeat elements, especially LTR/Gypsy in both J1 and J2. When comparing J1 against J2, there were 1222 down-methylated genes and 623 up-methylated genes. Methylated genes were significantly enriched in carbohydrate metabolism, biosynthesis of other secondary metabolites and amino acid metabolism pathways. The global DNA methylation level decreased from J1 to J2, especially in gene promoters, transcriptional termination regions and regions around CpG islands. We further investigated the expression patterns of 9 DNA methyltransferase-associated genes and 2 DNA demethyltransferase-associated genes from young to senescent cotyledons, which were down-regulated during cotyledon development. In this paper, we first reported that senescent cotton cotyledons exhibited lower DNA methylation levels, primarily due to decreased DNA methyltransferase activity and which also play important role in regulating secondary metabolite process.
[Mh] Termos MeSH primário: Cotilédone/genética
Metilação de DNA/genética
Gossypium/genética
Análise de Sequência de DNA
[Mh] Termos MeSH secundário: Metabolismo dos Carboidratos/genética
Cotilédone/crescimento & desenvolvimento
Cotilédone/metabolismo
Ilhas de CpG/genética
Regulação para Baixo
Ontologia Genética
Genes de Plantas/genética
Gossypium/crescimento & desenvolvimento
Gossypium/metabolismo
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170718
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0179141


  5 / 1685 MEDLINE  
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[PMID]:28551744
[Au] Autor:Huang Y; Kuang Z; Deng Z; Zhang R; Cao L
[Ad] Endereço:College of Fundamental Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, China.
[Ti] Título:Endophytic bacterial and fungal communities transmitted from cotyledons and germs in peanut (Arachis hypogaea L.) sprouts.
[So] Source:Environ Sci Pollut Res Int;24(19):16458-16464, 2017 Jul.
[Is] ISSN:1614-7499
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Seed-borne endophytes could be transmitted into sprouts. Whether this happened in peanuts and the difference between microbial taxa in peanut germs and cotyledons remain unknown. In this research, Illumina-based sequencing was employed to investigate the microbial taxa in peanut germs, cotyledons, and sprouts. Sulfur-oxidizing bacteria was isolated and inoculated into peanut sprouts, and then, the growth of peanut seedlings was measured. The results illustrated that diverse bacteria and fungi were detected in peanut germs, cotyledons, and sprouts. The number of bacterial OTUs declined with the germination from germs and cotyledons to sprouts. However, the number of fungal OTUs increased during the seedling procedure. Seed-borne dominant bacterial genera Halothiobacillus and Synechococcus and fungal genera Humicola, Emericella, and Penicillium were detected in sprouts. Based on the endophytic community information, the Halothiobacillus strains were isolated from sprouts. Pot experiments that illustrated the growth of peanut seedlings inoculated with the strain were promoted. These results provide new understanding into plant-microbe interactions in peanut and suggest that the selection for biocontrol agents based on mycobiome and bacteriome analysis is reliable and feasible compared with the present greenhouse selection.
[Mh] Termos MeSH primário: Arachis
Cotilédone
Endófitos
[Mh] Termos MeSH secundário: Germinação
Plântulas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170529
[St] Status:MEDLINE
[do] DOI:10.1007/s11356-017-9254-4


  6 / 1685 MEDLINE  
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[PMID]:28545103
[Au] Autor:Shi W; Bloomberg M; Li G; Su S; Jia L
[Ad] Endereço:Key Laboratory for Silviculture and Conservation, Ministry of Education, Beijing Forestry University, Beijing, China.
[Ti] Título:Combined effects of cotyledon excision and nursery fertilization on root growth, nutrient status and outplanting performance of Quercus variabilis container seedlings.
[So] Source:PLoS One;12(5):e0177002, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Artificial excision of the distal part of acorns in order to promote germination is well researched in oak seedling cultivation studies. However, studies of combined effects of cotyledon excision and nursery fertilization on container seedlings are lacking, especially for seedling root growth and outplanting performance. This study aimed to explore the main effects of cotyledon excision on Quercus variabilis seedling emergence characteristics and demonstrated the combined effects of cotyledon excision and nursery fertilization on seedling quality to improve Quercus variabilis seedling outplanting performance. Four cotyledon excision treatments and two classes of nursery fertilization were implemented. Seedling emergence was noted every week after sowing. Seedling dry mass, morphology, and nutrient status were assessed at the end of the nursery season. After the first outplanting season, the aforementioned measurements along with seedling survival were determined once again. The results showed that cotyledon excision generally induced greater and more rapid seedling emergence, but did not affect shoot emergence synchronicity. The highest total emergence and emergence rate occurred with Intermediate excision (1/2 of the distal end of acorn was excised). Effects of nutrient loss due to cotyledon excision on seedling quality and outplanting performance were somewhat compensated by nursery fertilization. Nursery fertilization promoted dry mass increment (the net increment from T0 to T2 for dry mass) for excised seedlings after outplanting, resulting in better performance for Slight (1/3 of the distal end of acorn was excised) and Intermediate excision treatments in the field. Thus we conclude Intermediate excision combined with reasonable nursery fertilization can be recommended for production of nursery grown seedlings for afforestation.
[Mh] Termos MeSH primário: Cotilédone/crescimento & desenvolvimento
Fertilizantes
Raízes de Plantas/crescimento & desenvolvimento
Quercus/efeitos dos fármacos
Quercus/crescimento & desenvolvimento
Plântulas/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Biomassa
Cotilédone/efeitos dos fármacos
Cotilédone/metabolismo
Raízes de Plantas/efeitos dos fármacos
Raízes de Plantas/metabolismo
Quercus/metabolismo
Plântulas/efeitos dos fármacos
Plântulas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fertilizers)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170908
[Lr] Data última revisão:
170908
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170526
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0177002


  7 / 1685 MEDLINE  
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[PMID]:28542293
[Au] Autor:Chen L; Lu D; Wang T; Li Z; Zhao Y; Jiang Y; Zhang Q; Cao Q; Fang K; Xing Y; Qin L
[Ad] Endereço:College of Plant Science and Technology, Beijing Collaborative Innovation Center for Eco-Environmental Improvement with Forestry and Fruit Trees, Beijing University of Agriculture, Beijing, China.
[Ti] Título:Identification and expression analysis of starch branching enzymes involved in starch synthesis during the development of chestnut (Castanea mollissima Blume) cotyledons.
[So] Source:PLoS One;12(5):e0177792, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Chinese chestnut (Castanea mollissima Blume) is native to China and distributes widely in arid and semi-arid mountain area with barren soil. As a perennial crop, chestnut is an alternative food source and acts as an important commercial nut tree in China. Starch is the major metabolite in nuts, accounting for 46 ~ 64% of the chestnut dry weight. The accumulation of total starch and amylopectin showed a similar increasing trend during the development of nut. Amylopectin contributed up to 76% of the total starch content at 80 days after pollination (DAP). The increase of total starch mainly results from amylopectin synthesis. Among genes associated with starch biosynthesis, CmSBEs (starch branching enzyme) showed significant increase during nut development. Two starch branching enzyme isoforms, CmSBE I and CmSBE II, were identified from chestnut cotyledon using zymogram analysis. CmSBE I and CmSBE II showed similar patterns of expression during nut development. The accumulations of CmSBE transcripts and proteins in developing cotyledons were characterized. The expressions of two CmSBE genes increased from 64 DAP and reached the highest levels at 77 DAP, and SBE activity reached its peak at 74 DAP. These results suggested that the CmSBE enzymes mainly contributed to amylopectin synthesis and influenced the amylopectin content in the developing cotyledon, which would be beneficial to chestnut germplasm selection and breeding.
[Mh] Termos MeSH primário: Cotilédone/enzimologia
Cotilédone/crescimento & desenvolvimento
Fagaceae/enzimologia
Fagaceae/crescimento & desenvolvimento
Proteínas de Plantas/metabolismo
Amido/biossíntese
[Mh] Termos MeSH secundário: Western Blotting
China
Eletroforese em Gel de Poliacrilamida
Perfilação da Expressão Gênica
Regulação da Expressão Gênica no Desenvolvimento
Regulação da Expressão Gênica de Plantas
Isoenzimas
Espectrometria de Massas
Polinização
Reação em Cadeia da Polimerase em Tempo Real
Amido/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Isoenzymes); 0 (Plant Proteins); 9005-25-8 (Starch)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170526
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0177792


  8 / 1685 MEDLINE  
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[PMID]:28235891
[Au] Autor:Marmon S; Sturtevant D; Herrfurth C; Chapman K; Stymne S; Feussner I
[Ad] Endereço:Albrecht-von-Haller Institute for Plant Sciences (S.M., C.H., I.F.) and Göttingen Center for Molecular Biosciences (I.F.), Department of Plant Biochemistry, Georg-August-University, 37077 Goettingen, Germany; sofia.marmon@slu.se.
[Ti] Título:Two Acyltransferases Contribute Differently to Linolenic Acid Levels in Seed Oil.
[So] Source:Plant Physiol;173(4):2081-2095, 2017 Apr.
[Is] ISSN:1532-2548
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Acyltransferases are key contributors to triacylglycerol (TAG) synthesis and, thus, are of great importance for seed oil quality. The effects of increased or decreased expression of ( ) or ( ) on seed lipid composition were assessed in several lines. Furthermore, in vitro assays of acyltransferases in microsomal fractions prepared from developing seeds of some of these lines were performed. Decreased expression of led to an increased percentage of 18:3 -3 without any change in total lipid content of the seed. The tri-18:3 TAG increase occurred predominantly in the cotyledon, as determined with matrix-assisted laser desorption/ionization-mass spectrometry, whereas species with two 18:3 -3 acyl groups were elevated in both cotyledon and embryonal axis. overexpression led to a relative increase of 18:2 -6 at the expense of 18:3 -3, also without affecting the total lipid content. Differential distributions of TAG species also were observed in different parts of the seed. The microsomal assays revealed that seeds have very high activity of diacylglycerol-phosphatidylcholine interconversion. The combination of analytical and biochemical data suggests that the higher 18:2 -6 content in the seed oil of the overexpressors is due to the channeling of fatty acids from phosphatidylcholine into TAG before being desaturated to 18:3 -3, caused by the high activity of PDAT in general and by PDAT specificity for 18:2 -6. The higher levels of 18:3 -3 in -silencing lines are likely due to the compensatory activity of a TAG-synthesizing enzyme with specificity for this acyl group and more desaturation of acyl groups occurring on phosphatidylcholine.
[Mh] Termos MeSH primário: Aciltransferases/metabolismo
Óleos Vegetais/metabolismo
Proteínas de Plantas/metabolismo
Sementes/metabolismo
Ácido alfa-Linolênico/metabolismo
[Mh] Termos MeSH secundário: Acil Coenzima A/metabolismo
Aciltransferases/genética
Brassicaceae/enzimologia
Brassicaceae/genética
Brassicaceae/metabolismo
Cotilédone/enzimologia
Cotilédone/genética
Cotilédone/metabolismo
Ácidos Graxos/análise
Ácidos Graxos/metabolismo
Regulação da Expressão Gênica de Plantas
Inativação Gênica
Isoenzimas/genética
Isoenzimas/metabolismo
Lipídeos/análise
Proteínas de Plantas/genética
Plantas Geneticamente Modificadas
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Sementes/enzimologia
Sementes/genética
Espectrometria de Massas por Ionização por Electrospray
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
Triglicerídeos/análise
Triglicerídeos/biossíntese
Ácido alfa-Linolênico/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acyl Coenzyme A); 0 (Fatty Acids); 0 (Isoenzymes); 0 (Lipids); 0 (Plant Oils); 0 (Plant Proteins); 0 (Triglycerides); 0RBV727H71 (alpha-Linolenic Acid); EC 2.3.- (Acyltransferases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170226
[St] Status:MEDLINE
[do] DOI:10.1104/pp.16.01865


  9 / 1685 MEDLINE  
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[PMID]:28235890
[Au] Autor:Kohzuma K; Sato Y; Ito H; Okuzaki A; Watanabe M; Kobayashi H; Nakano M; Yamatani H; Masuda Y; Nagashima Y; Fukuoka H; Yamada T; Kanazawa A; Kitamura K; Tabei Y; Ikeuchi M; Sakamoto W; Tanaka A; Kusaba M
[Ad] Endereço:Graduate School of Science, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8526, Japan (K.K., M.N., H.Y., Y.M., Y.N., M.K.).
[Ti] Título:The Non-Mendelian Green Cotyledon Gene in Soybean Encodes a Small Subunit of Photosystem II.
[So] Source:Plant Physiol;173(4):2138-2147, 2017 Apr.
[Is] ISSN:1532-2548
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Chlorophyll degradation plays important roles in leaf senescence including regulation of degradation of chlorophyll-binding proteins. Although most genes encoding enzymes of the chlorophyll degradation pathway have been identified, the regulation of their activity has not been fully understood. Green cotyledon mutants in legume are stay-green mutants, in which chlorophyll degradation is impaired during leaf senescence and seed maturation. Among them, the soybean ( ) green cotyledon gene is unique because it is maternally inherited. To isolate , we extensively sequenced the soybean chloroplast genome, and detected a 5-bp insertion causing a frame-shift in , which encodes one of the small subunits of photosystem II. Mutant tobacco plants ( ) with a disrupted generated using a chloroplast transformation technique had green senescent leaves, confirming that encodes PsbM. The phenotype of was very similar to that of mutant of chlorophyll reductase catalyzing the first step of chlorophyll degradation. In fact, chlorophyll -degrading activity in dark-grown and -knockout seedlings was significantly lower than that of wild-type plants. Our results suggest that PsbM is a unique protein linking photosynthesis in presenescent leaves with chlorophyll degradation during leaf senescence and seed maturation. Additionally, we discuss the origin of , which may have been selected during domestication of soybean.
[Mh] Termos MeSH primário: Cotilédone/genética
Complexo de Proteína do Fotossistema II/genética
Proteínas de Plantas/genética
Feijão de Soja/genética
[Mh] Termos MeSH secundário: Oxirredutases do Álcool/genética
Oxirredutases do Álcool/metabolismo
Sequência de Bases
Biocatálise
Western Blotting
Clorofila/metabolismo
Cloroplastos/genética
Cloroplastos/metabolismo
Cloroplastos/ultraestrutura
Cotilédone/metabolismo
Escuridão
Regulação da Expressão Gênica de Plantas
Microscopia Eletrônica de Transmissão
Mutação
Fenótipo
Complexo de Proteína do Fotossistema II/metabolismo
Folhas de Planta/genética
Folhas de Planta/metabolismo
Proteínas de Plantas/metabolismo
Subunidades Proteicas/genética
Subunidades Proteicas/metabolismo
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Homologia de Sequência do Ácido Nucleico
Feijão de Soja/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Photosystem II Protein Complex); 0 (Plant Proteins); 0 (Protein Subunits); 1406-65-1 (Chlorophyll); 5712ZB110R (chlorophyll b); EC 1.1.- (Alcohol Oxidoreductases); EC 1.1.1.- (chlorophyll b reductase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170226
[St] Status:MEDLINE
[do] DOI:10.1104/pp.16.01589


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[PMID]:28224915
[Au] Autor:Power IL; Dang PM; Sobolev VS; Orner VA; Powell JL; Lamb MC; Arias RS
[Ad] Endereço:United States Department of Agriculture, Agricultural Research Service, National Peanut Research Laboratory (NPRL), 1011 Forrester Dr. S. E., Dawson, GA 39842, USA. Electronic address: imanapower@yahoo.com.
[Ti] Título:Characterization of small RNA populations in non-transgenic and aflatoxin-reducing-transformed peanut.
[So] Source:Plant Sci;257:106-125, 2017 Apr.
[Is] ISSN:1873-2259
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:Aflatoxin contamination is a major constraint in food production worldwide. In peanut (Arachis hypogaea L.), these toxic and carcinogenic aflatoxins are mainly produced by Aspergillus flavus Link and A. parasiticus Speare. The use of RNA interference (RNAi) is a promising method to reduce or prevent the accumulation of aflatoxin in peanut seed. In this study, we performed high-throughput sequencing of small RNA populations in a control line and in two transformed peanut lines that expressed an inverted repeat targeting five genes involved in the aflatoxin-biosynthesis pathway and that showed up to 100% less aflatoxin B than the controls. The objective was to determine the putative involvement of the small RNA populations in aflatoxin reduction. In total, 41 known microRNA (miRNA) families and many novel miRNAs were identified. Among those, 89 known and 10 novel miRNAs were differentially expressed in the transformed lines. We furthermore found two small interfering RNAs derived from the inverted repeat, and 39 sRNAs that mapped without mismatches to the genome of A. flavus and were present only in the transformed lines. This information will increase our understanding of the effectiveness of RNAi and enable the possible improvement of the RNAi technology for the control of aflatoxins.
[Mh] Termos MeSH primário: Aflatoxinas/metabolismo
Arachis/genética
RNA de Plantas/genética
Transformação Genética
[Mh] Termos MeSH secundário: Sequência de Bases
Cotilédone/metabolismo
Perfilação da Expressão Gênica
Regulação da Expressão Gênica de Plantas
Marcadores Genéticos
Sequenciamento de Nucleotídeos em Larga Escala
MicroRNAs/genética
MicroRNAs/metabolismo
Plantas Geneticamente Modificadas
Interferência de RNA
RNA de Plantas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aflatoxins); 0 (Genetic Markers); 0 (MicroRNAs); 0 (RNA, Plant)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170320
[Lr] Data última revisão:
170320
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170223
[St] Status:MEDLINE



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