Base de dados : MEDLINE
Pesquisa : A18.024.875.750 [Categoria DeCS]
Referências encontradas : 1473 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 148 ir para página                         

  1 / 1473 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29317207
[Au] Autor:Imamura T; Takagi H; Miyazato A; Ohki S; Mizukoshi H; Mori M
[Ad] Endereço:Ishikawa Prefectural University, Nonoichi, Ishikawa, 921-8836, Japan. Electronic address: timamura@ishikawa-up.ac.jp.
[Ti] Título:Isolation and characterization of the betalain biosynthesis gene involved in hypocotyl pigmentation of the allotetraploid Chenopodium quinoa.
[So] Source:Biochem Biophys Res Commun;496(2):280-286, 2018 02 05.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In quinoa seedlings, the pigment betalain accumulates in the hypocotyl. To isolate the genes involved in betalain biosynthesis in the hypocotyl, we performed ethyl methanesulfonate (EMS) mutagenesis on the CQ127 variety of quinoa seedlings. While putative amaranthin and celosianin II primarily accumulate in the hypocotyls, this process produced a green hypocotyl mutant (ghy). This MutMap+ method using the quinoa draft genome revealed that the causative gene of the mutant is CqCYP76AD1-1. Our results indicated that the expression of CqCYP76AD1-1 was light-dependent. In addition, the transient expression of CqCYP76AD1-1 in Nicotiana benthamiana leaves resulted in the accumulation of betanin but not isobetanin, and the presence of a polymorphism in CqCYP76A1-2 in the CQ127 variety was shown to have resulted in its loss of function. These findings suggested that CqCYP76AD1-1 is involved in betalain biosynthesis during the hypocotyl pigmentation process in quinoa. To our knowledge, CqCYP76AD1-1 is the first quinoa gene identified by EMS mutagenesis using a draft gene sequence.
[Mh] Termos MeSH primário: O-Dealquilase 7-Alcoxicumarina/genética
Betalaínas/biossíntese
Chenopodium quinoa/genética
Regulação da Expressão Gênica de Plantas
Genes de Plantas
Hipocótilo/genética
[Mh] Termos MeSH secundário: O-Dealquilase 7-Alcoxicumarina/metabolismo
Sequência de Bases
Betacianinas/biossíntese
Chenopodium quinoa/efeitos dos fármacos
Chenopodium quinoa/crescimento & desenvolvimento
Chenopodium quinoa/metabolismo
Metanossulfonato de Etila/farmacologia
Hipocótilo/efeitos dos fármacos
Hipocótilo/crescimento & desenvolvimento
Hipocótilo/metabolismo
Luz
Mutagênese
Mutagênicos/farmacologia
Pigmentação
Folhas de Planta/efeitos dos fármacos
Folhas de Planta/genética
Folhas de Planta/crescimento & desenvolvimento
Folhas de Planta/metabolismo
Polimorfismo Genético
Tabaco/genética
Tabaco/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Betacyanins); 0 (Mutagens); 15167-84-7 (amaranthin betacyanin); 37279-84-8 (Betalains); 5YJC992ZP6 (betanin); 9H154DI0UP (Ethyl Methanesulfonate); EC 1.14.13.- (7-Alkoxycoumarin O-Dealkylase); EC 1.14.13.- (cytochrome P-450 CYP76B1 (Helianthus tuberosus))
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180111
[St] Status:MEDLINE


  2 / 1473 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29248583
[Au] Autor:Li Y; Zhang Y; Li M; Luo Q; Mallano AI; Jing Y; Zhang Y; Zhao L; Li W
[Ad] Endereço:Key Laboratory of Soybean Biology in Chinese Education Ministry (Northeastern Key Laboratory of Soybean Biology and Genetics & Breeding in Chinese Ministry of Agriculture), Northeast Agricultural University, Harbin 150030, China.
[Ti] Título:GmPLP1, a PAS/LOV protein, functions as a possible new type of blue light photoreceptor in soybean.
[So] Source:Gene;645:170-178, 2018 Mar 01.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Light is one of the most important environmental factors for the growth and development of plants. To adapt to changes in day length, the photoreception and transmission of the light signals in plants mainly depend on the various light receptor proteins. The PAS/LOV protein (PLP) has a PAS domain in the N-terminal and LOV domain in the C-terminal and has been confirmed as a new type of blue light receptor in Arabidopsis thaliana. However, the role of its counterpart in soybean remains largely unclear. In this study, the expression pattern of the GmPLP1 under different light qualities was determined by real-time RT-PCR analysis using the cultivar 'DongNong 42', a photosensitive soybean cultivar, suggesting that GmPLP1 was affected by the circadian clock and was a dark-induced gene. Moreover, the mRNA abundance increased significantly under blue light. Further analysis revealed that overexpression of GmPLP1 displayed the inhibition of hypocotyl elongation under blue light, and the expression of CRY1, CRY2, CKL3, CKL4, BIT1, and HY5 were simultaneously increased in GmPLP1-transgenic Arabidopsis, suggesting that the shortened hypocotyl was associated with the up-regulation of these genes. Taken together, our results suggest that GmPLP1, which is a new possible type of blue light photoreceptor in soybean, plays an important role in the blue light signaling pathway.
[Mh] Termos MeSH primário: Criptocromos/genética
Criptocromos/metabolismo
Feijão de Soja/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Ritmo Circadiano
Regulação da Expressão Gênica de Plantas
Hipocótilo/crescimento & desenvolvimento
Mutação
Proteínas de Plantas/genética
Proteínas de Plantas/metabolismo
Transdução de Sinais
Feijão de Soja/genética
Feijão de Soja/metabolismo
Regulação para Cima
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cryptochromes); 0 (Plant Proteins)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171218
[St] Status:MEDLINE


  3 / 1473 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28466186
[Au] Autor:Tai F; Lv S; Jiang P; Wang J; Feng J; Li Y
[Ad] Endereço:Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, No. 20 Nanxincun, Xiangshan, Beijing, 100093, China.
[Ti] Título:Establishment of a gene function analysis system for the euhalophyte Salicornia europaea L.
[So] Source:Plant Cell Rep;36(8):1251-1261, 2017 Aug.
[Is] ISSN:1432-203X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:KEY MESSAGE: A Salicornia europaea L. in vitro cell transformation system was developed and further applied to SeNHX1 function investigation. The exploration of salt-tolerant genes from halophyte has seriously been limited by the lack of self-dependent transformation system. Here, an Agrobacterium tumefaciens-mediated in vitro cell transformation system of euhalophyte Salicornia europaea L. was developed. Calli derived from hypocotyl of S. europaea were co-cultured for 3 days with Agrobacterium at OD ranging from 1.0 to 1.5 and then selected with 25 mg/L hygromycin (Hyg). The transformed cells were identified from Hyg positive calli by GUS assay and qRT-PCR, and the transformation efficiency was up to 74.4%. The practicality of this system was further tested via genetic manipulation of S. europaea Na /H antiporter 1 (SeNHX1) gene by creating the overexpressing, silencing, and empty vector cells. Survival ratio and Na distribution under salt treatment showed obvious differences in SeNHX1-overexpressing, -silencing, and empty vector cells, indicating the feasibility of this system to analyze gene function. This investigation is enlightening for studies in other non-model plants lacking of self-dependent transformation system.
[Mh] Termos MeSH primário: Chenopodiaceae/metabolismo
[Mh] Termos MeSH secundário: Agrobacterium tumefaciens/genética
Chenopodiaceae/efeitos dos fármacos
Chenopodiaceae/genética
Hipocótilo/efeitos dos fármacos
Hipocótilo/genética
Hipocótilo/metabolismo
Proteínas de Plantas/genética
Proteínas de Plantas/metabolismo
Plantas Geneticamente Modificadas/efeitos dos fármacos
Plantas Geneticamente Modificadas/genética
Plantas Geneticamente Modificadas/metabolismo
Tolerância a Sal/genética
Plantas Tolerantes a Sal/efeitos dos fármacos
Plantas Tolerantes a Sal/genética
Plantas Tolerantes a Sal/metabolismo
Cloreto de Sódio/farmacologia
Trocadores de Sódio-Hidrogênio/genética
Trocadores de Sódio-Hidrogênio/metabolismo
Transformação Genética/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Proteins); 0 (Sodium-Hydrogen Exchangers); 451W47IQ8X (Sodium Chloride)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180102
[Lr] Data última revisão:
180102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1007/s00299-017-2150-z


  4 / 1473 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28747176
[Au] Autor:Xu X; Chen M; Ji J; Xu Q; Qi X; Chen X
[Ad] Endereço:Department of horticulture, School of Horticulture and Plant Protection, Yangzhou University, 48 wenhui eastroad, Yangzhou, Jiangsu, 225009, China.
[Ti] Título:Comparative RNA-seq based transcriptome profiling of waterlogging response in cucumber hypocotyls reveals novel insights into the de novo adventitious root primordia initiation.
[So] Source:BMC Plant Biol;17(1):129, 2017 07 26.
[Is] ISSN:1471-2229
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Waterlogging is a serious abiotic stress to plant growth because it results in the decline in the supplement of oxygen to submerged tissues. Although cucumber (Cucumis sativus L.) is sensitive to waterlogging, its ability to generate adventitious roots (ARs) facilitates gas diffusion and increases plant survival when the oxygen concentration is decreased. To gain a better understanding of the molecular mechanisms that enable de novo AR primordia emergence upon waterlogging, the RNA sequencing-based transcriptomic responses of two contrasting cucumber genotypes, Zaoer-N (waterlogging tolerant) and Pepino (waterlogging sensitive), which differed in their abilities to form AR were compared. RESULTS: More than 27,000 transcripts were detected in cucumber hypocotyls, from which 1494 and 1766 genes in 'Zaoer-N' and 'Pepino', respectively, were differentially expressed 2 days after waterlogging. The significant positive correlation between RNA sequencing data and a qPCR analysis indicated that the identified genes were credible. A comparative analysis revealed that genes functioning in carbohydrate mobilization, nitrate assimilation, hormone production and signaling pathways, transcription factors and cell division might contribute to the waterlogging-triggered AR primordia initiation. Ethylene was determined to be an important plant hormone responsible for the cucumber ARs initiation. Additionally, genes encoding cytochrome P450, ankyrin repeat-containing proteins and sulfite oxidases were determined as important in waterlogging acclimation. CONCLUSION: This research broadens our understanding of the mechanism underlying waterlogging-triggered ARs emergence, and provides valuable information for the breeding of cucumber with enhanced waterlogging tolerance.
[Mh] Termos MeSH primário: Cucumis sativus/genética
Hipocótilo/fisiologia
Raízes de Plantas/crescimento & desenvolvimento
RNA de Plantas
[Mh] Termos MeSH secundário: Cucumis sativus/crescimento & desenvolvimento
Perfilação da Expressão Gênica
Regulação da Expressão Gênica de Plantas
Reguladores de Crescimento de Planta/fisiologia
Proteínas de Plantas/metabolismo
Estresse Fisiológico/genética
Fatores de Transcrição/metabolismo
Água
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Plant Growth Regulators); 0 (Plant Proteins); 0 (RNA, Plant); 0 (Transcription Factors); 059QF0KO0R (Water)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171220
[Lr] Data última revisão:
171220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170728
[St] Status:MEDLINE
[do] DOI:10.1186/s12870-017-1081-8


  5 / 1473 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27770560
[Au] Autor:Zhang Y; Yu Q; Jiang N; Yan X; Wang C; Wang Q; Liu J; Zhu M; Bednarek SY; Xu J; Pan J
[Ad] Endereço:College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua, 321004, China.
[Ti] Título:Clathrin regulates blue light-triggered lateral auxin distribution and hypocotyl phototropism in Arabidopsis.
[So] Source:Plant Cell Environ;40(1):165-176, 2017 Jan.
[Is] ISSN:1365-3040
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Phototropism is the process by which plants grow towards light in order to maximize the capture of light for photosynthesis, which is particularly important for germinating seedlings. In Arabidopsis, hypocotyl phototropism is predominantly triggered by blue light (BL), which has a profound effect on the establishment of asymmetric auxin distribution, essential for hypocotyl phototropism. Two auxin efflux transporters ATP-binding cassette B19 (ABCB19) and PIN-formed 3 (PIN3) are known to mediate the effect of BL on auxin distribution in the hypocotyl, but the details for how BL triggers PIN3 lateralization remain poorly understood. Here, we report a critical role for clathrin in BL-triggered, PIN3-mediated asymmetric auxin distribution in hypocotyl phototropism. We show that unilateral BL induces relocalization of clathrin in the hypocotyl. Loss of clathrin light chain 2 (CLC2) and CLC3 affects endocytosis and lateral distribution of PIN3 thereby impairing BL-triggered establishment of asymmetric auxin distribution and consequently, phototropic bending. Conversely, auxin efflux inhibitors N-1-naphthylphthalamic acid and 2,3,5-triiodobenzoic acid affect BL-induced relocalization of clathrin, endocytosis and lateralization of PIN3 as well as asymmetric distribution of auxin. These results together demonstrate an important interplay between auxin and clathrin function that dynamically regulates BL-triggered hypocotyl phototropism in Arabidopsis.
[Mh] Termos MeSH primário: Arabidopsis/fisiologia
Arabidopsis/efeitos da radiação
Clatrina/metabolismo
Hipocótilo/fisiologia
Ácidos Indolacéticos/metabolismo
Luz
Fototropismo/efeitos da radiação
[Mh] Termos MeSH secundário: Proteínas de Arabidopsis/metabolismo
Endocitose/efeitos da radiação
Hipocótilo/efeitos da radiação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (Clathrin); 0 (Indoleacetic Acids)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171212
[Lr] Data última revisão:
171212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161023
[St] Status:MEDLINE
[do] DOI:10.1111/pce.12854


  6 / 1473 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28981578
[Au] Autor:Fry SC
[Ad] Endereço:The Edinburgh Cell Wall Group, Institute of Molecular Plant Sciences, The University of Edinburgh, Daniel Rutherford Building, The King's Buildings, Max Born Crescent, Edinburgh EH9 3BF, UK.
[Ti] Título:Potassium, not lepidimoide, is the principal 'allelochemical' of cress-seed exudate that promotes amaranth hypocotyl elongation.
[So] Source:Ann Bot;120(4):511-520, 2017 Oct 17.
[Is] ISSN:1095-8290
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Background and Aims: Imbibed cress ( Lepidium sativum L.) seeds exude 'allelochemicals' that promote excessive hypocotyl elongation and inhibit root growth in neighbouring competitors, e.g. amaranth ( Amaranthus caudatus L.) seedlings. The major hypocotyl promoter has recently been shown not to be the previously suggested acidic disaccharide, lepidimoic acid (LMA), a fragment of the pectic polysaccharide domain rhamnogalacturonan-I. The nature of the hypocotyl promoter has now been re-assessed. Methods: Low-molecular weight cress-seed exudate (LCSE) was fractionated by high-voltage electrophoresis, and components with different charge:mass ratios were tested for effects on dark-grown amaranth seedlings. Further samples of LCSE were size-fractionated by gel permeation chromatography, and active fractions were analysed electrophoretically. Key Results: The LCSE strongly promoted amaranth hypocotyl elongation. The active principle was hydrophilic and, unlike LMA, stable to hot acid. After electrophoresis at pH 6·5, the only fractions that strongly promoted hypocotyl elongation were those with a very high positive charge:mass ratio, migrating towards the cathode 3-4 times faster than glucosamine. Among numerous naturally occurring cations tested, the only one with such a high mobility was potassium. K + was present in LCSE at approx. 4 m m , and pure KCl (1-10 m m ) strongly promoted amaranth hypocotyl elongation. No other cation tested (including Na + , spermidine and putrescine) had this effect. The peak of bioactivity from a gel permeation chromatography column exactly coincided with the peak of K + . Conclusions: The major 'allelopathic' substance present in cress-seed exudate that stimulates hypocotyl elongation in neighbouring seedlings is the inorganic cation, K + , not the oligosaccharin LMA.
[Mh] Termos MeSH primário: Amaranthus/crescimento & desenvolvimento
Dissacarídeos/fisiologia
Exsudatos e Transudatos/fisiologia
Hipocótilo/crescimento & desenvolvimento
Lepidium sativum/fisiologia
Potássio/fisiologia
Sementes/metabolismo
[Mh] Termos MeSH secundário: Amaranthus/metabolismo
Cromatografia em Gel
Eletroforese/métodos
Exsudatos e Transudatos/química
Hipocótilo/metabolismo
Lepidium sativum/metabolismo
Sementes/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Disaccharides); 145039-76-5 (lepidimoide); RWP5GA015D (Potassium)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171006
[St] Status:MEDLINE
[do] DOI:10.1093/aob/mcx081


  7 / 1473 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28827312
[Au] Autor:Roy Choudhury S; Pandey S
[Ad] Endereço:From the Donald Danforth Plant Science Center, St. Louis, Missouri 63132.
[Ti] Título:Recently duplicated plant heterotrimeric Gα proteins with subtle biochemical differences influence specific outcomes of signal-response coupling.
[So] Source:J Biol Chem;292(39):16188-16198, 2017 Sep 29.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Heterotrimeric G-proteins, comprising Gα, Gß, and Gγ subunits, regulate key signaling processes in eukaryotes. The Gα subunit determines the status of signaling by switching between inactive GDP-bound and active GTP-bound forms. Unlike animal systems, in which multiple Gα proteins with variable biochemical properties exist, plants have fewer, highly similar Gα subunits that have resulted from recent genome duplications. These proteins exhibit subtle differences in their GTP-binding, GDP/GTP-exchange, and GTP-hydrolysis activities, but the extent to which these differences contribute to affect plant signaling and development remains unknown. To evaluate this, we expressed native and engineered Gα proteins from soybean in an Gα-null background and studied their effects on modulating a range of developmental and hormonal signaling phenotypes. Our results indicated that inherent biochemical differences in these highly similar Gα proteins are biologically relevant, and some proteins are more flexible than others in influencing the outcomes of specific signals. These observations suggest that alterations in the rate of the G-protein cycle itself may contribute to the specificity of response regulation in plants by affecting the duration of active signaling and/or by the formation of distinct protein-protein complexes. In species such as having a single canonical Gα, this rate could be affected by regulatory proteins in the presence of specific signals, whereas in plants with multiple Gα proteins, an even more complex regulation may exist, which likely contributes to the specificity of signal-response coupling.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/metabolismo
Arabidopsis/metabolismo
Subunidades alfa de Proteínas de Ligação ao GTP/metabolismo
Subunidades beta da Proteína de Ligação ao GTP/metabolismo
Plantas Geneticamente Modificadas/metabolismo
Transdução de Sinais
Proteínas de Soja/metabolismo
Feijão de Soja/enzimologia
[Mh] Termos MeSH secundário: Substituição de Aminoácidos
Arabidopsis/genética
Arabidopsis/crescimento & desenvolvimento
Proteínas de Arabidopsis/química
Proteínas de Arabidopsis/genética
Subunidades alfa de Proteínas de Ligação ao GTP/química
Subunidades alfa de Proteínas de Ligação ao GTP/genética
Subunidades beta da Proteína de Ligação ao GTP/química
Subunidades beta da Proteína de Ligação ao GTP/genética
Duplicação Gênica
Técnicas de Inativação de Genes
Hipocótilo/genética
Hipocótilo/crescimento & desenvolvimento
Hipocótilo/metabolismo
Isoenzimas/química
Isoenzimas/genética
Isoenzimas/metabolismo
Mutagênese Sítio-Dirigida
Mutação
Folhas de Planta/genética
Folhas de Planta/crescimento & desenvolvimento
Folhas de Planta/metabolismo
Plantas Geneticamente Modificadas/genética
Plantas Geneticamente Modificadas/crescimento & desenvolvimento
Regiões Promotoras Genéticas
Multimerização Proteica
Proteínas Recombinantes/química
Proteínas Recombinantes/metabolismo
Proteínas de Soja/química
Proteínas de Soja/genética
Feijão de Soja/genética
Técnicas do Sistema de Duplo-Híbrido
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (AGB1 protein, Arabidopsis); 0 (Arabidopsis Proteins); 0 (GTP-Binding Protein alpha Subunits); 0 (GTP-Binding Protein beta Subunits); 0 (Isoenzymes); 0 (Recombinant Proteins); 0 (Soybean Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170823
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M117.793380


  8 / 1473 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28683099
[Au] Autor:Zhang Y; Li C; Zhang J; Wang J; Yang J; Lv Y; Yang N; Liu J; Wang X; Palfalvi G; Wang G; Zheng L
[Ad] Endereço:Laboratory of Medicinal Plant, School of Basic Medicine, Hubei University of Medicine, Shiyan, China.
[Ti] Título:Dissection of HY5/HYH expression in Arabidopsis reveals a root-autonomous HY5-mediated photomorphogenic pathway.
[So] Source:PLoS One;12(7):e0180449, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:ELONGATED HYPOCOTYL 5 (HY5), a member of the bZIP gene family, is a positive regulator of the light signaling pathway in Arabidopsis thaliana. Whereas the hy5 mutant exhibits an elongated hypocotyl when grown in the light, the hy5 homolog (hyh) mutant does not. Although the functions of HY5 and HYH in light-mediated seedling development have been revealed, the tissue-specific expression patterns of HY5 and HYH and their interconnected regulation are largely unknown. Here, we report that HY5 regulates HYH expression in roots and contributes to root growth under different light conditions. We generated HY5 and HYH transcriptional and translational fusion reporter lines to investigate their expression patterns. HY5 was constitutively expressed in all root tissues, while HYH was predominantly expressed in root xylem cells. Root growth after a dark-to-light transition was perturbed in the hy5 and hy5hyh mutant lines, but not in the hyh mutant line, indicating that HY5 plays a major role in light-regulated root growth. Light-induced HY5/HYH expression occurred autonomously in roots. HYH expression in roots was decreased in the hy5 mutant, suggesting that HY5 regulates HYH expression. Collectively, these results indicate that an organ-specific HY5-mediated pathway controls root photomorphogenic development independently of light signaling in the shoot.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/genética
Arabidopsis/genética
Fatores de Transcrição de Zíper de Leucina Básica/genética
Proteínas de Transporte/genética
Regulação da Expressão Gênica de Plantas
Transdução de Sinal Luminoso
Proteínas Nucleares/genética
Raízes de Plantas/genética
[Mh] Termos MeSH secundário: Arabidopsis/metabolismo
Proteínas de Arabidopsis/metabolismo
Fatores de Transcrição de Zíper de Leucina Básica/metabolismo
Proteínas de Transporte/metabolismo
Genes Reporter
Glucuronidase/genética
Glucuronidase/metabolismo
Proteínas de Fluorescência Verde/genética
Proteínas de Fluorescência Verde/metabolismo
Hipocótilo/genética
Hipocótilo/metabolismo
Luz
Mutação
Proteínas Nucleares/metabolismo
Especificidade de Órgãos
Fotossíntese/genética
Raízes de Plantas/metabolismo
Biossíntese de Proteínas
Proteínas Recombinantes de Fusão/genética
Proteínas Recombinantes de Fusão/metabolismo
Plântulas/genética
Plântulas/metabolismo
Transcrição Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (Basic-Leucine Zipper Transcription Factors); 0 (Carrier Proteins); 0 (HY5 protein, Arabidopsis); 0 (HYH protein, Arabidopsis); 0 (Nuclear Proteins); 0 (Recombinant Fusion Proteins); 147336-22-9 (Green Fluorescent Proteins); EC 3.2.1.31 (Glucuronidase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170707
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0180449


  9 / 1473 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28671250
[Au] Autor:Carneiro VQ; Silva GN; Cruz CD; Carneiro PCS; Nascimento M; Carneiro JES
[Ad] Endereço:Departamento de Biologia Geral, Universidade Federal de Viçosa, Viçosa, MG, Brasil vqcarneiro@gmail.com.
[Ti] Título:Artificial neural networks as auxiliary tools for the improvement of bean plant architecture.
[So] Source:Genet Mol Res;16(2), 2017 Jun 29.
[Is] ISSN:1676-5680
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:Classification using a scale of visual notes is a strategy used to select erect bean plants in order to improve bean plant architectures. Use of morphological traits associated with the phenotypic expression of bean architecture in classification procedures may enhance selection. The objective of this study was to evaluate the potential of artificial neural networks (ANNs) as auxiliary tools in the improvement of bean plant architecture. Data from 19 lines were evaluated for 22 traits, in 2007 and 2009 winter crops. Hypocotyl diameter and plant height were selected for analysis through ANNs. For classification purposes, these lines were separated into two groups, determined by the plant architecture notes. The predictive ability of ANNs was evaluated according to two scenarios to predict the plant architecture - training with 2007 data and validating in 2009 data (scenario 1), and vice versa (scenario 2). For this, ANNs were trained and validated using data from replicates of the evaluated lines for hypocotyl diameter individually, or together with the mean height of plants in the plot. In each scenario, the use of data from replicates or line means was evaluated for prediction through previously trained and validated ANNs. In both scenarios, ANNs based on hypocotyl diameter and mean height of plants were superior, since the error rates obtained were lower than those obtained using hypocotyl diameter only. Lower apparent error rates were verified in both scenarios for prediction when data on the means of the evaluated traits were submitted to better trained and validated ANNs.
[Mh] Termos MeSH primário: Redes Neurais (Computação)
Fenótipo
Melhoramento Vegetal/métodos
Feijão de Soja/genética
[Mh] Termos MeSH secundário: Hipocótilo/genética
Hipocótilo/crescimento & desenvolvimento
Modelos Genéticos
Característica Quantitativa Herdável
Feijão de Soja/anatomia & histologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170704
[St] Status:MEDLINE
[do] DOI:10.4238/gmr16029500


  10 / 1473 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28542545
[Au] Autor:Diniz I; Figueiredo A; Loureiro A; Batista D; Azinheira H; Várzea V; Pereira AP; Gichuru E; Moncada P; Guerra-Guimarães L; Oliveira H; Silva MDC
[Ad] Endereço:Centro de Investigação das Ferrugens do Cafeeiro (CIFC), Instituto Superior de Agronomia (ISA), Universidade de Lisboa, Oeiras, Portugal.
[Ti] Título:A first insight into the involvement of phytohormones pathways in coffee resistance and susceptibility to Colletotrichum kahawae.
[So] Source:PLoS One;12(5):e0178159, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Understanding the molecular mechanisms underlying coffee-pathogen interactions are of key importance to aid disease resistance breeding efforts. In this work the expression of genes involved in salicylic acid (SA), jasmonic acid (JA) and ethylene (ET) pathways were studied in hypocotyls of two coffee varieties challenged with the hemibiotrophic fungus Colletotrichum kahawae, the causal agent of Coffee Berry Disease. Based on a cytological analysis, key time-points of the infection process were selected and qPCR was used to evaluate the expression of phytohormones biosynthesis, reception and responsive-related genes. The resistance to C. kahawae was characterized by restricted fungal growth associated with early accumulation of phenolic compounds in the cell walls and cytoplasmic contents, and deployment of hypersensitive reaction. Similar responses were detected in the susceptible variety, but in a significantly lower percentage of infection sites and with no apparent effect on disease development. Gene expression analysis suggests a more relevant involvement of JA and ET phytohormones than SA in this pathosystem. An earlier and stronger activation of the JA pathway observed in the resistant variety, when compared with the susceptible one, seems to be responsible for the successful activation of defense responses and inhibition of fungal growth. For the ET pathway, the down or non-regulation of ET receptors in the resistant variety, together with a moderate expression of the responsive-related gene ERF1, indicates that this phytohormone may be related with other functions besides the resistance response. However, in the susceptible variety, the stronger activation of ERF1 gene at the beginning of the necrotrophic phase, suggests the involvement of ET in tissue senescence. As far as we know, this is the first attempt to unveil the role of phytohormones in coffee-C. kahawae interactions, thus contributing to deepen our understanding on the complex mechanisms of plant signaling and defense.
[Mh] Termos MeSH primário: Café/genética
Café/microbiologia
Regulação da Expressão Gênica de Plantas
Genes de Plantas
Doenças das Plantas/genética
Doenças das Plantas/microbiologia
Reguladores de Crescimento de Planta/genética
[Mh] Termos MeSH secundário: Café/metabolismo
Colletotrichum/fisiologia
Resistência à Doença
Seres Humanos
Hipocótilo/genética
Hipocótilo/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Coffee); 0 (Plant Growth Regulators)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170526
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0178159



página 1 de 148 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde