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[PMID]:28457165
[Au] Autor:de Simone A; Hubbard R; de la Torre NV; Velappan Y; Wilson M; Considine MJ; Soppe WJJ; Foyer CH
[Ad] Endereço:1 Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds , Leeds, United Kingdom .
[Ti] Título:Redox Changes During the Cell Cycle in the Embryonic Root Meristem of Arabidopsis thaliana.
[So] Source:Antioxid Redox Signal;27(18):1505-1519, 2017 Dec 20.
[Is] ISSN:1557-7716
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:AIMS: The aim of this study was to characterize redox changes in the nuclei and cytosol occurring during the mitotic cell cycle in the embryonic roots of germinating Arabidopsis seedlings, and to determine how redox cycling was modified in mutants with a decreased capacity for ascorbate synthesis. RESULTS: Using an in vivo reduction-oxidation (redox) reporter (roGFP2), we show that transient oxidation of the cytosol and the nuclei occurred at G1 in the synchronized dividing cells of the Arabidopsis root apical meristem, with reduction at G2 and mitosis. This redox cycle was absent from low ascorbate mutants in which nuclei were significantly more oxidized than controls. The cell cycle-dependent increase in nuclear size was impaired in the ascorbate-deficient mutants, which had fewer cells per unit area in the root proliferation zone. The transcript profile of the dry seeds and size of the imbibed seeds was strongly influenced by low ascorbate but germination, dormancy release and seed aging characteristics were unaffected. INNOVATION: These data demonstrate the presence of a redox cycle within the plant cell cycle and that the redox state of the nuclei is an important factor in cell cycle progression. CONCLUSIONS: Controlled oxidation is a key feature of the early stages of the plant cell cycle. However, sustained mild oxidation restricts nuclear functions and impairs progression through the cell cycle leading to fewer cells in the root apical meristem. Antioxid. Redox Signal. 27, 1505-1519.
[Mh] Termos MeSH primário: Arabidopsis/crescimento & desenvolvimento
Meristema/embriologia
Oxirredução
Proteínas de Plantas/genética
[Mh] Termos MeSH secundário: Arabidopsis/embriologia
Arabidopsis/genética
Ciclo Celular
Núcleo Celular/genética
Núcleo Celular/metabolismo
Citosol/metabolismo
Regulação da Expressão Gênica no Desenvolvimento
Regulação da Expressão Gênica de Plantas
Germinação
Meristema/genética
Raízes de Plantas/embriologia
Raízes de Plantas/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Proteins)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE
[do] DOI:10.1089/ars.2016.6959


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[PMID]:28460115
[Au] Autor:Fan Z; Cai Z; Shan J; Yang J
[Ad] Endereço:State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, and Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering, Guangxi University, Nanning 530004, China.
[Ti] Título:Letter to the Editor: Bud Position and Carbohydrate Play a More Significant Role than Light Condition in the Developmental Transition between Rhizome Buds and Aerial Shoot Buds of Oryza longistaminata.
[So] Source:Plant Cell Physiol;58(8):1281-1282, 2017 08 01.
[Is] ISSN:1471-9053
[Cp] País de publicação:Japan
[La] Idioma:eng
[Mh] Termos MeSH primário: Oryza
Rizoma
[Mh] Termos MeSH secundário: Carboidratos
Regulação da Expressão Gênica de Plantas
Meristema
Brotos de Planta
[Pt] Tipo de publicação:LETTER; RESEARCH SUPPORT, NON-U.S. GOV'T; COMMENT
[Nm] Nome de substância:
0 (Carbohydrates)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE
[do] DOI:10.1093/pcp/pcx061


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[PMID]:29287997
[Au] Autor:Machado KDC; Sousa LQ; Lima DJB; Soares BM; Cavalcanti BC; Maranhão SS; Noronha JDC; Rodrigues DJ; Militão GCG; Chaves MH; Vieira-Júnior GM; Pessoa C; Moraes MO; Sousa JMCE; Melo-Cavalcante AAC; Ferreira PMP
[Ad] Endereço:Postgraduate Program in Pharmaceutical Sciences, Federal University of Piauí, Teresina, Brazil.
[Ti] Título:Marinobufagin, a molecule from poisonous frogs, causes biochemical, morphological and cell cycle changes in human neoplasms and vegetal cells.
[So] Source:Toxicol Lett;285:121-131, 2018 Mar 15.
[Is] ISSN:1879-3169
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Skin toad secretion present physiologically active molecules to protect them against microorganisms, predators and infections. This work detailed the antiproliferative action of marinobufagin on tumor and normal lines, investigate its mechanism on HL-60 leukemia cells and its toxic effects on Allium cepa meristematic cells. Initially, cytotoxic action was assessed by colorimetric assays. Next, HL-60 cells were analyzed by morphological and flow cytometry techniques and growing A. cepa roots were examined after 72 h exposure. Marinobufagin presented high antiproliferative action against all human tumor lines [IC values ranging from 0.15 (leukemia) to 7.35 (larynx) µM] and it failed against human erythrocytes and murine lines. Human normal peripheral blood mononuclear cells (PBMC) were up to 72.5-fold less sensitive [IC 10.88 µM] to marinobufagin than HL-60 line, but DNA strand breaks were no detected. Leukemia treaded cells exhibited cell viability reduction, DNA fragmentation, phosphatidylserine externalization, binucleation, nuclear condensation and cytoplasmic vacuoles. Marinobufagin also reduced the growth of A. cepa roots (EC : 7.5 µM) and mitotic index, caused cell cycle arrest and chromosomal alterations (micronuclei, delays and C-metaphases) in meristematic cells. So, to find out partially targeted natural molecules on human leukemia cells, like marinobufagin, is an amazing and stimulating way to continue the battle against cancer.
[Mh] Termos MeSH primário: Antineoplásicos/farmacologia
Bufanolídeos/farmacologia
Ciclo Celular/efeitos dos fármacos
Quebras de DNA
Cebolas/efeitos dos fármacos
[Mh] Termos MeSH secundário: Adolescente
Adulto
Animais
Antineoplásicos/isolamento & purificação
Antineoplásicos/toxicidade
Bufanolídeos/isolamento & purificação
Bufanolídeos/toxicidade
Bufonidae/metabolismo
Sobrevivência Celular/efeitos dos fármacos
Ensaio Cometa
Relação Dose-Resposta a Droga
Eritrócitos/efeitos dos fármacos
Células HL-60
Voluntários Saudáveis
Hemólise/efeitos dos fármacos
Seres Humanos
Leucócitos Mononucleares/efeitos dos fármacos
Meristema/citologia
Meristema/efeitos dos fármacos
Meristema/genética
Micronúcleos com Defeito Cromossômico/induzido quimicamente
Cebolas/citologia
Cebolas/genética
Pele/secreção
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Bufanolides); 3KBT25GV2B (marinobufagenin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180219
[Lr] Data última revisão:
180219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171231
[St] Status:MEDLINE


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[PMID]:29269486
[Au] Autor:Barghetti A; Sjögren L; Floris M; Paredes EB; Wenkel S; Brodersen P
[Ad] Endereço:Department of Biology, University of Copenhagen, DK-2200 Copenhagen N, Denmark.
[Ti] Título:Heat-shock protein 40 is the key farnesylation target in meristem size control, abscisic acid signaling, and drought resistance.
[So] Source:Genes Dev;31(22):2282-2295, 2017 11 15.
[Is] ISSN:1549-5477
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Protein farnesylation is central to molecular cell biology. In plants, protein farnesyl transferase mutants are pleiotropic and exhibit defective meristem organization, hypersensitivity to the hormone abscisic acid, and increased drought resistance. The precise functions of protein farnesylation in plants remain incompletely understood because few relevant farnesylated targets have been identified. Here, we show that defective farnesylation of a single factor-heat-shock protein 40 (HSP40), encoded by the and genes-is sufficient to confer ABA hypersensitivity, drought resistance, late flowering, and enlarged meristems, indicating that altered function of chaperone client proteins underlies most farnesyl transferase mutant phenotypes. We also show that expression of an abiotic stress-related microRNA (miRNA) regulon controlled by the transcription factor SPL7 requires HSP40 farnesylation. Expression of a truncated SPL7 form mimicking its activated proteolysis fragment of the membrane-bound SPL7 precursor partially restores accumulation of SPL7-dependent miRNAs in farnesyl transferase mutants. These results implicate the pathway directing SPL7 activation from its membrane-bound precursor as an important target of farnesylated HSP40, consistent with our demonstration that HSP40 farnesylation facilitates its membrane association. The results also suggest that altered gene regulation via select miRNAs contributes to abiotic stress-related phenotypes of farnesyl transferase mutants.
[Mh] Termos MeSH primário: Ácido Abscísico/fisiologia
Proteínas de Arabidopsis/metabolismo
Proteínas de Choque Térmico HSP40/metabolismo
Meristema/metabolismo
[Mh] Termos MeSH secundário: Arabidopsis/anatomia & histologia
Arabidopsis/genética
Arabidopsis/crescimento & desenvolvimento
Arabidopsis/metabolismo
Proteínas de Arabidopsis/genética
Membrana Celular/metabolismo
Proteínas de Ligação a DNA/metabolismo
Secas
Farnesiltranstransferase/genética
Proteínas de Choque Térmico HSP90/genética
Meristema/anatomia & histologia
MicroRNAs/metabolismo
Mutação
Prenilação
Transdução de Sinais
Fatores de Transcrição/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (DNA-Binding Proteins); 0 (ERA1 protein, Arabidopsis); 0 (HSP40 Heat-Shock Proteins); 0 (HSP90 Heat-Shock Proteins); 0 (MicroRNAs); 0 (SPL7 protein, Arabidopsis); 0 (Transcription Factors); 72S9A8J5GW (Abscisic Acid); EC 2.5.1.29 (Farnesyltranstransferase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180202
[Lr] Data última revisão:
180202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171223
[St] Status:MEDLINE
[do] DOI:10.1101/gad.301242.117


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[PMID]:28981580
[Au] Autor:Velappan Y; Signorelli S; Considine MJ
[Ad] Endereço:The UWA Institute of Agriculture, The University of Western Australia, Perth, WA 6009, Australia.
[Ti] Título:Cell cycle arrest in plants: what distinguishes quiescence, dormancy and differentiated G1?
[So] Source:Ann Bot;120(4):495-509, 2017 Oct 17.
[Is] ISSN:1095-8290
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Background: Quiescence is a fundamental feature of plant life, which enables plasticity, renewal and fidelity of the somatic cell line. Cellular quiescence is defined by arrest in a particular phase of the cell cycle, typically G1 or G2; however, the regulation of quiescence and proliferation can also be considered across wider scales in space and time. As such, quiescence is a defining feature of plant development and phenology, from meristematic stem cell progenitors to terminally differentiated cells, as well as dormant or suppressed seeds and buds. While the physiology of each of these states differs considerably, each is referred to as 'cell cycle arrest' or 'G1 arrest'. Scope: Here the physiology and molecular regulation of (1) meristematic quiescence, (2) dormancy and (3) terminal differentiation (cell cycle exit) are considered in order to determine whether and how the molecular decisions guiding these nuclear states are distinct. A brief overview of the canonical cell cycle regulators is provided, and the genetic and genomic, as well as physiological, evidence is considered regarding two primary questions: (1) Are the canonical cell cycle regulators superior or subordinate in the regulation of quiescence? (2) Are these three modes of quiescence governed by distinct molecular controls? Conclusion: Meristematic quiescence, dormancy and terminal differentiation are each predominantly characterized by G1 arrest but regulated distinctly, at a level largely superior to the canonical cell cycle. Meristematic quiescence is intrinsically linked to non-cell-autonomous regulation of meristem cell identity, and particularly through the influence of ubiquitin-dependent proteolysis, in partnership with reactive oxygen species, abscisic acid and auxin. The regulation of terminal differentiation shares analogous features with meristematic quiescence, albeit with specific activators and a greater role for cytokinin signalling. Dormancy meanwhile appears to be regulated at the level of chromatin accessibility, by Polycomb group-type histone modifications of particular dormancy genes.
[Mh] Termos MeSH primário: Pontos de Checagem do Ciclo Celular/fisiologia
Desenvolvimento Vegetal/fisiologia
Dormência de Plantas/fisiologia
[Mh] Termos MeSH secundário: Fase G1/fisiologia
Meristema/crescimento & desenvolvimento
Meristema/fisiologia
Raízes de Plantas/crescimento & desenvolvimento
Raízes de Plantas/fisiologia
Brotos de Planta/crescimento & desenvolvimento
Brotos de Planta/fisiologia
Plantas
Fase de Repouso do Ciclo Celular/fisiologia
Fase S/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171006
[St] Status:MEDLINE
[do] DOI:10.1093/aob/mcx082


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[PMID]:28902851
[Au] Autor:Fuller AW; Young P; Pierce BD; Kitson-Finuff J; Jain P; Schneider K; Lazar S; Taran O; Palmer AG; Lynn DG
[Ad] Endereço:Departments of Biology and Chemistry, Emory University, Atlanta, GA, United States of America.
[Ti] Título:Redox-mediated quorum sensing in plants.
[So] Source:PLoS One;12(9):e0182655, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The rhizosphere, the narrow zone of soil around plant roots, is a complex network of interactions between plants, bacteria, and a variety of other organisms. The absolute dependence on host-derived signals, or xenognosins, to regulate critical developmental checkpoints for host commitment in the obligate parasitic plants provides a window into the rhizosphere's chemical dynamics. These sessile intruders use H2O2 in a process known as semagenesis to chemically modify the mature root surfaces of proximal host plants and generate p-benzoquinones (BQs). The resulting redox-active signaling network regulates the spatial and temporal commitments necessary for host attachment. Recent evidence from non-parasites, including Arabidopsis thaliana, establishes that reactive oxygen species (ROS) production regulates similar redox circuits related to root recognition, broadening xenognosins' role beyond the parasites. Here we compare responses to the xenognosin dimethoxybenzoquinone (DMBQ) between the parasitic plant Striga asiatica and the non-parasitic A. thaliana. Exposure to DMBQ simulates the proximity of a mature root surface, stimulating an increase in cytoplasmic Ca2+ concentration in both plants, but leads to remarkably different phenotypic responses in the parasite and non-parasite. In S. asiatica, DMBQ induces development of the host attachment organ, the haustorium, and decreases ROS production at the root tip, while in A. thaliana, ROS production increases and further growth of the root tip is arrested. Obstruction of Ca2+ channels and the addition of antioxidants both lead to a decrease in the DMBQ response in both parasitic and non-parasitic plants. These results are consistent with Ca2+ regulating the activity of NADPH oxidases, which in turn sustain the autocatalytic production of ROS via an external quinone/hydroquinone redox cycle. Mechanistically, this chemistry is similar to black and white photography with the emerging dynamic reaction-diffusion network laying the foundation for the precise temporal and spatial control underlying rhizosphere architecture.
[Mh] Termos MeSH primário: Arabidopsis
Interações Hospedeiro-Parasita
Fenômenos Fisiológicos Vegetais
Percepção de Quorum/fisiologia
[Mh] Termos MeSH secundário: Arabidopsis/efeitos dos fármacos
Arabidopsis/crescimento & desenvolvimento
Arabidopsis/metabolismo
Arabidopsis/parasitologia
Benzoquinonas/farmacologia
Sinalização do Cálcio/efeitos dos fármacos
Interações Hospedeiro-Parasita/efeitos dos fármacos
Peróxido de Hidrogênio/metabolismo
Peróxido de Hidrogênio/farmacologia
Meristema/efeitos dos fármacos
Meristema/crescimento & desenvolvimento
Meristema/metabolismo
Meristema/parasitologia
Oxirredução
Raízes de Plantas/efeitos dos fármacos
Raízes de Plantas/crescimento & desenvolvimento
Raízes de Plantas/metabolismo
Raízes de Plantas/parasitologia
Espécies Reativas de Oxigênio/metabolismo
Striga/efeitos dos fármacos
Striga/crescimento & desenvolvimento
Striga/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Benzoquinones); 0 (Reactive Oxygen Species); BBX060AN9V (Hydrogen Peroxide)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170914
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182655


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[PMID]:28726096
[Au] Autor:Shchennikova AV; Shulga OA; Beletsky AV; Filyushin MA; Kochieva EZ; Ravin NV; Skryabin KG
[Ad] Endereço:Institute of Bioengineering, Research Center of Biotechnology, Russian Academy of Sciences, Moscow, 119071, Russia. shchennikova@yandex.ru.
[Ti] Título:Identification and characterization of the flower meristem identity gene MhyLFY in mycoheterotrophic plant Monotropa hypopitys.
[So] Source:Dokl Biochem Biophys;474(1):204-208, 2017 May.
[Is] ISSN:1608-3091
[Cp] País de publicação:Russia (Federation)
[La] Idioma:eng
[Ab] Resumo:The gene encoding the transcription factor LEAFY was identified in the genome of the mycoheterotrophic plant, pinesap Monotropa hypopitys. In the transcriptomes of roots, bracts, and flowers of flowering pinesaps, the MhyLFY gene expression was absent. These data suggest the conservativeness of the LFY-dependent mechanism of flower meristem identity and flower formation in heterotrophic species with some differences associated to the specificity of development and the structure of such plants. The pinesap flowering under the control of the transcription factor MhyLFY may be initiated either in an embryonic inflorescence during spring dormancy release of adventitious root buds or in an inflorescence of a growing reproductive stem after photoperiodic induction.
[Mh] Termos MeSH primário: Ericaceae/genética
Flores/genética
Meristema/genética
Proteínas de Plantas/genética
Proteínas de Plantas/metabolismo
Fatores de Transcrição/genética
Fatores de Transcrição/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Evolução Molecular
Filogenia
Proteínas de Plantas/química
Fatores de Transcrição/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Proteins); 0 (Transcription Factors)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170721
[St] Status:MEDLINE
[do] DOI:10.1134/S1607672917030103


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[PMID]:28710125
[Au] Autor:Wen F; Curlango-Rivera G; Huskey DA; Xiong Z; Hawes MC
[Ad] Endereço:Department of Soil, Water and Environmental Science, 429 Shantz Building #38, The University of Arizona, Tucson, Arizona 85721, USA.
[Ti] Título:Visualization of extracellular DNA released during border cell separation from the root cap.
[So] Source:Am J Bot;104(7):970-978, 2017 Jul.
[Is] ISSN:1537-2197
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PREMISE OF THE STUDY: Root border cells are programmed to separate from the root cap as it penetrates the soil environment, where the cells actively secrete >100 extracellular proteins into the surrounding mucilage. The detached cells function in defense of the root tip by an extracellular trapping process that also requires DNA, as in mammalian white blood cells. Trapping in animals and plants is reversed by treatment with DNase, which results in increased infection. The goal of this study was to evaluate the role of DNA in the structural integrity of extracellular structures released as border cells disperse from the root tip upon contact with water. METHODS: DNA stains including crystal violet, toluidine blue, Hoechst 33342, DAPI, and SYTOX green were added to root tips to visualize the extracellular mucilage as it absorbed water and border cell populations dispersed. DNase I was used to assess structural changes occurring when extracellular DNA was degraded. KEY RESULTS: Complex masses associated with living border cells were immediately evident in response to each stain, including those that are specific for DNA. Treating with DNase I dramatically altered the appearance of the extracellular structures and their association with border cells. No extracellular DNA was found in association with border cells killed by freezing or high-speed centrifugation. This observation is consistent with the hypothesis that, as with border cell extracellular proteins, DNA is secreted by living cells. CONCLUSION: DNA is an integral component of border cell extracellular traps.
[Mh] Termos MeSH primário: DNA de Plantas/química
Meristema/citologia
Ervilhas/citologia
Raízes de Plantas/citologia
Zea mays/citologia
[Mh] Termos MeSH secundário: Meristema/crescimento & desenvolvimento
Ervilhas/crescimento & desenvolvimento
Raízes de Plantas/crescimento & desenvolvimento
Zea mays/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Plant)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170716
[St] Status:MEDLINE
[do] DOI:10.3732/ajb.1700142


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[PMID]:28594957
[Au] Autor:Maniga A; Ghisaura S; Perrotta L; Marche MG; Cella R; Albani D
[Ad] Endereço:Department of Agriculture, University of Sassari, Sassari, Italy.
[Ti] Título:Distinctive features and differential regulation of the DRTS genes of Arabidopsis thaliana.
[So] Source:PLoS One;12(6):e0179338, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In plants and protists, dihydrofolate reductase (DHFR) and thymidylate synthase (TS) are part of a bifunctional enzyme (DRTS) that allows efficient recycling of the dihydrofolate resulting from TS activity. Arabidopsis thaliana possesses three DRTS genes, called AtDRTS1, AtDRTS2 and AtDRTS3, that are located downstream of three members of the sec14-like SFH gene family. In this study, a characterization of the AtDRTS genes identified alternatively spliced transcripts coding for AtDRTS isoforms which may account for monofunctional DHFR enzymes supporting pathways unrelated to DNA synthesis. Moreover, we discovered a complex differential regulation of the AtDRTS genes that confirms the expected involvement of the AtDRTS genes in cell proliferation and endoreduplication, but indicates also functions related to other cellular activities. AtDRTS1 is widely expressed in both meristematic and differentiated tissues, whereas AtDRTS2 expression is almost exclusively limited to the apical meristems and AtDRTS3 is preferentially expressed in the shoot apex, in stipules and in root cap cells. The differential regulation of the AtDRTS genes is associated to distinctive promoter architectures and the expression of AtDRTS1 in the apical meristems is strictly dependent on the presence of an intragenic region that includes the second intron of the gene. Upon activation of cell proliferation in germinating seeds, the activity of the AtDRTS1 and AtDRTS2 promoters in meristematic cells appears to be maximal at the G1/S phase of the cell cycle. In addition, the promoters of AtDRTS2 and AtDRTS3 are negatively regulated through E2F cis-acting elements and both genes, but not AtDRTS1, are downregulated in plants overexpressing the AtE2Fa factor. Our study provides new information concerning the function and the regulation of plant DRTS genes and opens the way to further investigations addressing the importance of folate synthesis with respect to specific cellular activities.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/genética
Arabidopsis/enzimologia
Arabidopsis/genética
Regulação da Expressão Gênica de Plantas
Genes de Plantas
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Arabidopsis/citologia
Arabidopsis/efeitos dos fármacos
Proteínas de Arabidopsis/química
Proteínas de Arabidopsis/metabolismo
Ciclo Celular/efeitos dos fármacos
Ciclo Celular/genética
Proliferação Celular/efeitos dos fármacos
Proliferação Celular/genética
Simulação por Computador
Citocininas/farmacologia
Regulação para Baixo/efeitos dos fármacos
Regulação para Baixo/genética
Perfilação da Expressão Gênica
Regulação da Expressão Gênica de Plantas/efeitos dos fármacos
Germinação/efeitos dos fármacos
Germinação/genética
Glucuronidase/metabolismo
Ácidos Indolacéticos/farmacologia
Íntrons/genética
Isoenzimas/química
Isoenzimas/genética
Isoenzimas/metabolismo
Meristema/efeitos dos fármacos
Meristema/genética
Fases de Leitura Aberta/genética
Plantas Geneticamente Modificadas
Regiões Promotoras Genéticas
Sementes/efeitos dos fármacos
Sementes/genética
Sementes/crescimento & desenvolvimento
Frações Subcelulares/efeitos dos fármacos
Frações Subcelulares/enzimologia
Transformação Genética/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (Cytokinins); 0 (Indoleacetic Acids); 0 (Isoenzymes); EC 3.2.1.31 (Glucuronidase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170609
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0179338


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[PMID]:28586647
[Au] Autor:Gutiérrez-Alanís D; Yong-Villalobos L; Jiménez-Sandoval P; Alatorre-Cobos F; Oropeza-Aburto A; Mora-Macías J; Sánchez-Rodríguez F; Cruz-Ramírez A; Herrera-Estrella L
[Ad] Endereço:Metabolic Engineering Group, Unidad de Genómica Avanzada, Laboratorio Nacional de Genómica para la Biodiversidad (LANGEBIO) del Centro de Investigación y Estudios Avanzados, Km. 9.6 Libramiento Norte Carr. Irapuato-León, 36821 Irapuato, Guanajuato, Mexico; Instituto de Biotecnología, Universidad Nac
[Ti] Título:Phosphate Starvation-Dependent Iron Mobilization Induces CLE14 Expression to Trigger Root Meristem Differentiation through CLV2/PEPR2 Signaling.
[So] Source:Dev Cell;41(5):555-570.e3, 2017 Jun 05.
[Is] ISSN:1878-1551
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Low inorganic phosphate (Pi) availability causes terminal differentiation of the root apical meristem (RAM), a phenomenon known as root meristem exhaustion or determined growth. Here, we report that the CLE14 peptide acts as a key player in this process. Low Pi stress induces iron mobilization in the RAM through the action of LPR1/LPR2, causing expression of CLE14 in the proximal meristem region. CLV2 and PEPR2 receptors perceive CLE14 and trigger RAM differentiation, with concomitant downregulation of SHR/SCR and PIN/AUXIN pathway. Our results reveal multiple steps of the molecular mechanism of one of the most physiologically important root nutrient responses.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/metabolismo
Arabidopsis/crescimento & desenvolvimento
Diferenciação Celular
Ferro/metabolismo
Proteínas de Membrana/metabolismo
Meristema/crescimento & desenvolvimento
Fosfatos/deficiência
Raízes de Plantas/crescimento & desenvolvimento
Proteínas Serina-Treonina Quinases/metabolismo
[Mh] Termos MeSH secundário: Arabidopsis/metabolismo
Proteínas de Arabidopsis/genética
Regulação da Expressão Gênica de Plantas
Proteínas de Membrana/genética
Meristema/metabolismo
Raízes de Plantas/metabolismo
Proteínas Serina-Treonina Quinases/genética
Transdução de Sinais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (CLE14 protein, Arabidopsis); 0 (CLV2 protein, Arabidopsis); 0 (Membrane Proteins); 0 (Phosphates); E1UOL152H7 (Iron); EC 2.7.11.1 (PEPR2 protein, Arabidopsis); EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170912
[Lr] Data última revisão:
170912
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170607
[St] Status:MEDLINE



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