Base de dados : MEDLINE
Pesquisa : A18.024.875.875.500 [Categoria DeCS]
Referências encontradas : 134 [refinar]
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  1 / 134 MEDLINE  
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[PMID]:28453813
[Au] Autor:Zhou D; Du Q; Chen J; Wang Q; Zhang D
[Ad] Endereço:Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, P.R. China.
[Ti] Título:Identification and allelic dissection uncover roles of lncRNAs in secondary growth of Populus tomentosa.
[So] Source:DNA Res;24(5):473-486, 2017 Oct 01.
[Is] ISSN:1756-1663
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Long non-coding RNAs (lncRNAs) function in various biological processes. However, their roles in secondary growth of plants remain poorly understood. Here, 15,691 lncRNAs were identified from vascular cambium, developing xylem, and mature xylem of Populus tomentosa with high and low biomass using RNA-seq, including 1,994 lncRNAs that were differentially expressed (DE) among the six libraries. 3,569 cis-regulated and 3,297 trans-regulated protein-coding genes were predicted as potential target genes (PTGs) of the DE lncRNAs to participate in biological regulation. Then, 476 and 28 lncRNAs were identified as putative targets and endogenous target mimics (eTMs) of Populus known microRNAs (miRNAs), respectively. Genome re-sequencing of 435 individuals from a natural population of P. tomentosa found 34,015 single nucleotide polymorphisms (SNPs) within 178 lncRNA loci and 522 PTGs. Single-SNP associations analysis detected 2,993 associations with 10 growth and wood-property traits under additive and dominance model. Epistasis analysis identified 17,656 epistatic SNP pairs, providing evidence for potential regulatory interactions between lncRNAs and their PTGs. Furthermore, a reconstructed epistatic network, representing interactions of 8 lncRNAs and 15 PTGs, might enrich regulation roles of genes in the phenylpropanoid pathway. These findings may enhance our understanding of non-coding genes in plants.
[Mh] Termos MeSH primário: Regulação da Expressão Gênica de Plantas
Polimorfismo de Nucleotídeo Único
Populus/metabolismo
Característica Quantitativa Herdável
RNA Longo não Codificante/fisiologia
[Mh] Termos MeSH secundário: Câmbio/genética
Câmbio/crescimento & desenvolvimento
Câmbio/metabolismo
Epistasia Genética
Estudos de Associação Genética
Populus/genética
Populus/crescimento & desenvolvimento
RNA Longo não Codificante/genética
RNA de Plantas/genética
RNA de Plantas/fisiologia
Análise de Sequência de DNA
Análise de Sequência de RNA
Transcriptoma
Xilema/genética
Xilema/crescimento & desenvolvimento
Xilema/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Long Noncoding); 0 (RNA, Plant)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:180109
[Lr] Data última revisão:
180109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1093/dnares/dsx018


  2 / 134 MEDLINE  
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[PMID]:28406933
[Au] Autor:Zhang YF; Li GL; Wang XF; Sun YQ; Zhang SY
[Ad] Endereço:Sugar Beet Physiological Research Institute, Inner Mongolia Agricultural University, Hohhot, China.
[Ti] Título:Transcriptomic profiling of taproot growth and sucrose accumulation in sugar beet (Beta vulgaris L.) at different developmental stages.
[So] Source:PLoS One;12(4):e0175454, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In sugar beet (Beta vulgaris L.), taproot weight and sucrose content are the important determinants of yield and quality. However, high yield and low sucrose content are two tightly bound agronomic traits. The advances in next-generation sequencing technology and the publication of sugar beet genome have provided a method for the study of molecular mechanism underlying the regulation of these two agronomic traits. In this work, we performed comparative transcriptomic analyses in the high taproot yield cultivar SD13829 and the high sucrose content cultivar BS02 at five developmental stages. More than 50,000,000 pair-end clean reads for each library were generated. When taproot turned into the rapid growth stage at the growth stage of 82 days after emergence (DAE), eighteen enriched gene ontology (GO) terms, including cell wall, cytoskeleton, and enzyme linked receptor protein signaling pathway, occurred in both cultivars. Differentially expressed genes (DEGs) of paired comparison in both cultivars were enriched in the cell wall GO term. For pathway enrichment analyses of DEGs that were respectively generated at 82 DAE compared to 59 DAE (the earlier developmental stage before taproot turning into the rapid growth stage), plant hormone signal transduction pathway was enriched. At 82 DAE, the rapid enlarging stage of taproot, several transcription factor family members were up-regulated in both cultivars. An antagonistic expression of brassinosteroid- and auxin-related genes was also detected. In SD13829, the growth strategy was relatively focused on cell enlargement promoted by brassinosteroid signaling, whereas in BS02, it was relatively focused on secondarily cambial cell division regulated by cytokinin, auxin and brassinosteroid signaling. Taken together, our data demonstrate that the weight and sucrose content of taproot rely on its growth strategy, which is controlled by brassinosteroid, auxin, cytokinin, and gibberellin.
[Mh] Termos MeSH primário: Beta vulgaris/crescimento & desenvolvimento
Regulação da Expressão Gênica de Plantas/fisiologia
Proteínas de Plantas/biossíntese
Raízes de Plantas/crescimento & desenvolvimento
Sacarose/metabolismo
Transcriptoma/fisiologia
[Mh] Termos MeSH secundário: Câmbio/metabolismo
Perfilação da Expressão Gênica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Proteins); 57-50-1 (Sucrose)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170504
[Lr] Data última revisão:
170504
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170414
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0175454


  3 / 134 MEDLINE  
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[PMID]:28218997
[Au] Autor:Denis E; Kbiri N; Mary V; Claisse G; Conde E Silva N; Kreis M; Deveaux Y
[Ad] Endereço:Saclay Plant Science, Institut de Biologie des Plantes, Univ. Paris-Sud, CNRS, Orsay, 91405, France.
[Ti] Título:WOX14 promotes bioactive gibberellin synthesis and vascular cell differentiation in Arabidopsis.
[So] Source:Plant J;90(3):560-572, 2017 May.
[Is] ISSN:1365-313X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Procambial and cambial stem cells provide the initial cells that allow the formation of vascular tissues. WOX4 and WOX14 have been shown to act redundantly to promote procambial cell proliferation and differentiation. Gibberellins (GAs), which have an important role in wood formation, also stimulate cambial cell division. Here we show that the loss of WOX14 function phenocopies some traits of GA-deficient mutants that can be complemented by exogenous GA application, whereas WOX14 overexpression stimulates the expression of GA3ox anabolism genes and represses GA2ox catabolism genes, promoting the accumulation of bioactive GA. More importantly, our data clearly indicate that WOX14 but not WOX4 promotes vascular cell differentiation and lignification in inflorescence stems of Arabidopsis.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/metabolismo
Arabidopsis/metabolismo
Giberelinas/metabolismo
Proteínas de Homeodomínio/metabolismo
[Mh] Termos MeSH secundário: Arabidopsis/citologia
Arabidopsis/genética
Proteínas de Arabidopsis/genética
Câmbio/metabolismo
Diferenciação Celular/efeitos dos fármacos
Regulação da Expressão Gênica de Plantas/genética
Regulação da Expressão Gênica de Plantas/fisiologia
Proteínas de Homeodomínio/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (Gibberellins); 0 (Homeodomain Proteins); 0 (WOX14 protein, Arabidopsis)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170628
[Lr] Data última revisão:
170628
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170221
[St] Status:MEDLINE
[do] DOI:10.1111/tpj.13513


  4 / 134 MEDLINE  
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[PMID]:28187161
[Au] Autor:Oles V; Panchenko A; Smertenko A
[Ad] Endereço:Department of Mathematics, Washington State University, Pullman, Washington, United States of America.
[Ti] Título:Modeling hormonal control of cambium proliferation.
[So] Source:PLoS One;12(2):e0171927, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Rise of atmospheric CO2 is one of the main causes of global warming. Catastrophic climate change can be avoided by reducing emissions and increasing sequestration of CO2. Trees are known to sequester CO2 during photosynthesis, and then store it as wood biomass. Thus, breeding of trees with higher wood yield would mitigate global warming as well as augment production of renewable construction materials, energy, and industrial feedstock. Wood is made of cellulose-rich xylem cells produced through proliferation of a specialized stem cell niche called cambium. Importance of cambium in xylem cells production makes it an ideal target for the tree breeding programs; however our knowledge about control of cambium proliferation remains limited. The morphology and regulation of cambium are different from those of stem cell niches that control axial growth. For this reason, translating the knowledge about axial growth to radial growth has limited use. Furthermore, genetic approaches cannot be easily applied because overlaying tissues conceal cambium from direct observation and complicate identification of mutants. To overcome the paucity of experimental tools in cambium biology, we constructed a Boolean network CARENET (CAmbium REgulation gene NETwork) for modelling cambium activity, which includes the key transcription factors WOX4 and HD-ZIP III as well as their potential regulators. Our simulations predict that: (1) auxin, cytokinin, gibberellin, and brassinosteroids act cooperatively in promoting transcription of WOX4 and HD-ZIP III; (2) auxin and cytokinin pathways negatively regulate each other; (3) hormonal pathways act redundantly in sustaining cambium activity; (4) individual cambium cells can have diverse molecular identities. CARENET can be extended to include components of other signalling pathways and be integrated with models of xylem and phloem differentiation. Such extended models would facilitate breeding trees with higher wood yield.
[Mh] Termos MeSH primário: Câmbio/crescimento & desenvolvimento
Proliferação Celular
Citocininas/metabolismo
Giberelinas/metabolismo
Ácidos Indolacéticos/metabolismo
Modelos Biológicos
[Mh] Termos MeSH secundário: Câmbio/citologia
Câmbio/metabolismo
Proteínas de Plantas/genética
Proteínas de Plantas/metabolismo
Fatores de Transcrição/genética
Fatores de Transcrição/metabolismo
Árvores/crescimento & desenvolvimento
Árvores/metabolismo
Árvores/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokinins); 0 (Gibberellins); 0 (Indoleacetic Acids); 0 (Plant Proteins); 0 (Transcription Factors)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170809
[Lr] Data última revisão:
170809
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170211
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0171927


  5 / 134 MEDLINE  
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[PMID]:27900471
[Au] Autor:Siebers T; Catarino B; Agusti J
[Ad] Endereço:Department of Plant Sciences, University of Oxford, South Parks Road, Oxford, OX1 3RB, UK.
[Ti] Título:Identification and expression analyses of new potential regulators of xylem development and cambium activity in cassava (Manihot esculenta).
[So] Source:Planta;245(3):539-548, 2017 Mar.
[Is] ISSN:1432-2048
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:MAIN CONCLUSIONS: We have identified new potential regulators of xylem cell-type determination and cellular proliferation in cassava and studied their expression in roots. Results are highly relevant for cassava biotechnology. Cassava's root system is composed of two types of root that coexist in every individual: the fibrous and the storage roots. Whether a root becomes fibrous or storage depends on the xylem cell types that it develops: fibrous roots develop xylem fibres and vessels while storage roots develop parenchyma xylem, the starch-storing tissue. A crucial question in cassava root development is how the specific xylem cell types differentiate and proliferate in the fibrous and storage roots. Using phylogenetic, protein sequence and synteny analyses we identified (1) MeVND6, MeVND7.1, MeVND7.2, MeNST3.1 and MeNST3.2 as the potential cassava orthologues of the Arabidopsis regulators of xylem cell type determination AtVND6, AtVND7 and AtNST3; and (2) MeWOX4.1 and MeWOX4.2 as the potential cassava orthologues of the Arabidopsis cambium regulator AtWOX4. Fibrous and storage roots were anatomically characterised and tested for the expression of the identified genes. Results revealed that (1) MeVND7.1 and MeVND7.2 are expressed in the fibrous but not in the storage roots; (2) MeVND6 shows low expression in both root types; (3) MeNST3.1 is not expressed in the fibrous or storage roots, while MeNST3.2 is highly expressed in both root-types and (4) MeWOX4.1 and, to a higher level, MeWOX4.2 are expressed in both the fibrous and storage roots. Results open new avenues for research in cassava root development and for food security-oriented biotechnology programmes.
[Mh] Termos MeSH primário: Câmbio/crescimento & desenvolvimento
Câmbio/genética
Regulação da Expressão Gênica de Plantas
Manihot/crescimento & desenvolvimento
Manihot/genética
Xilema/crescimento & desenvolvimento
Xilema/genética
[Mh] Termos MeSH secundário: Genes de Plantas
Filogenia
Proteínas de Plantas/genética
Proteínas de Plantas/metabolismo
Raízes de Plantas/anatomia & histologia
Homologia de Sequência de Aminoácidos
Sintenia/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Proteins)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171005
[Lr] Data última revisão:
171005
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161201
[St] Status:MEDLINE
[do] DOI:10.1007/s00425-016-2623-2


  6 / 134 MEDLINE  
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[PMID]:27889518
[Au] Autor:Jin F; Li J; Ding Q; Wang QS; He XQ
[Ad] Endereço:Northeast Agricultural University, Harbin 150040, China.
[Ti] Título:Proteomic analysis provides insights into changes in the central metabolism of the cambium during dormancy release in poplar.
[So] Source:J Plant Physiol;208:26-39, 2017 Jan.
[Is] ISSN:1618-1328
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Seasonal cycling of growth and dormancy is an important feature for the woody plants growing in temperate zone, and dormancy is an effective strategy for surviving the winter stress. But the mechanisms of dormancy maintenance and its release are still not clear, especially little information is available with regard to the changes of proteome during the process. A better understanding in the function of proteins and their related metabolic pathways would expand our knowledge of the mechanisms of dormancy maintenance and its release in trees. In this study, we employed the isobaric tags for relative and absolute quantification (iTRAQ) approach with LC-MS/MS analysis to investigate the protein profile changes during dormancy release in poplar. In addition, the change of lipid, total insoluble carbohydrates and starch granules in the cambium was investigated by histochemical methods. A total of 3789 proteins were identified in poplar cambial tissues, 1996 of them were significantly altered during the dormancy release. Most of the altered proteins involved in signaling, phytohormone, energy metabolism, stress and secondary metabolism by functional analysis. Our data shows that the lipid metabolism proteins changed significantly both in the release stage of eco- and endodormancy, while the changes of carbohydrate metabolism proteins were mainly in endo-dormancy release stage. Moreover, histochemical results were consistent with the proteomic data. Our results reveal diverse stage-specific metabolism changes during the dormancy-release process induced by chilling in poplar, which provided new information regarding the regulation mechanisms of dormancy maintenance and its release in trees.
[Mh] Termos MeSH primário: Câmbio/fisiologia
Regulação da Expressão Gênica de Plantas
Populus/fisiologia
Proteômica
Transdução de Sinais
[Mh] Termos MeSH secundário: Vias Biossintéticas
Metabolismo dos Carboidratos
Parede Celular/metabolismo
Metabolismo Energético
Metabolismo dos Lipídeos
Oxirredução
Reguladores de Crescimento de Planta/metabolismo
Proteínas de Plantas/metabolismo
Polissacarídeos/metabolismo
Populus/citologia
Plântulas/citologia
Plântulas/fisiologia
Estresse Fisiológico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Growth Regulators); 0 (Plant Proteins); 0 (Polysaccharides)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170425
[Lr] Data última revisão:
170425
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161128
[St] Status:MEDLINE


  7 / 134 MEDLINE  
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[PMID]:26739992
[Au] Autor:de Vasconcellos TJ; Da Cunha M; Callado CH
[Ad] Endereço:Programa de Pós-graduação em Biologia Vegetal, Instituto de Biologia Roberto Alcantara Gomes, Laboratório de Anatomia Vegetal, Universidade do Estado do Rio de Janeiro, Rua São Francisco Xavier, 524-PHLC, sala 224, 20550-013, Rio de Janeiro, RJ, Brazil.
[Ti] Título:A comparative study of cambium histology of Ceiba speciosa (A. St.-Hil.) Ravenna (Malvaceae) under urban pollution.
[So] Source:Environ Sci Pollut Res Int;24(13):12049-12062, 2017 May.
[Is] ISSN:1614-7499
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Air pollution is considered to be one of the main causes of forest decline. The cambium is responsible for increase in tree girth, and its functioning is determined by environmental pressures. This study compared cambium histology of Ceiba speciosa (A. St.-Hil.) Ravenna (Malvaceae) in polluted and preserved sites in the Atlantic Rainforest domain. Samples were obtained during periods of cambial activity and dormancy and were processed and examined according to standard light microscopy techniques. In addition to differences typically observed in cambium during periods of activity and dormancy, the fusiform initials were shorter in trees of the polluted site. Furthermore, cambial rays were shorter, but larger, in the polluted site. It should be noted that all parameters related to cambial rays showed significant differences between the study sites. This is the first report of the effects of pollution on cambial activity in a South American species. The results suggest a tolerance of C. speciosa to pollution and reveal this species to be an important biomarker for environmental monitoring studies.
[Mh] Termos MeSH primário: Câmbio/anatomia & histologia
Ceiba/efeitos dos fármacos
Poluição Ambiental
[Mh] Termos MeSH secundário: Brasil
Câmbio/efeitos dos fármacos
Ceiba/anatomia & histologia
Cidades
Monitoramento Ambiental
Árvores
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171104
[Lr] Data última revisão:
171104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160108
[St] Status:MEDLINE
[do] DOI:10.1007/s11356-015-6012-3


  8 / 134 MEDLINE  
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[PMID]:27322968
[Au] Autor:Kim H; Kojima M; Choi D; Park S; Matsui M; Sakakibara H; Hwang I
[Ad] Endereço:Department of Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Korea.
[Ti] Título:Overexpression of INCREASED CAMBIAL ACTIVITY, a putative methyltransferase, increases cambial activity and plant growth.
[So] Source:J Integr Plant Biol;58(11):874-889, 2016 Nov.
[Is] ISSN:1744-7909
[Cp] País de publicação:China (Republic : 1949- )
[La] Idioma:eng
[Ab] Resumo:Cambial activity is a prerequisite for secondary growth in plants; however, regulatory factors controlling the activity of the secondary meristem in radial growth remain elusive. Here, we identified INCREASED CAMBIAL ACTIVITY (ICA), a gene encoding a putative pectin methyltransferase, which could function as a modulator for the meristematic activity of fascicular and interfascicular cambium in Arabidopsis. An overexpressing transgenic line, 35S::ICA, showed accelerated stem elongation and radial thickening, resulting in increased accumulation of biomass, and increased levels of cytokinins (CKs) and gibberellins (GAs). Expression of genes encoding pectin methylesterases involved in pectin modification together with pectin methyltransferases was highly induced in 35S::ICA, which might contribute to an increase of methanol emission as a byproduct in 35S::ICA. Methanol treatment induced the expression of GA- or CK-responsive genes and stimulated plant growth. Overall, we propose that ectopic expression of ICA increases cambial activity by regulating CK and GA homeostasis, and methanol emission, eventually leading to stem elongation and radial growth in the inflorescence stem.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/genética
Proteínas de Arabidopsis/metabolismo
Arabidopsis
Expressão Gênica
Metiltransferases/genética
Metiltransferases/metabolismo
[Mh] Termos MeSH secundário: Arabidopsis/enzimologia
Arabidopsis/genética
Arabidopsis/crescimento & desenvolvimento
Arabidopsis/metabolismo
Câmbio/genética
Câmbio/crescimento & desenvolvimento
Citocininas/metabolismo
Giberelinas/metabolismo
Plantas Geneticamente Modificadas/genética
Plantas Geneticamente Modificadas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (Cytokinins); 0 (Gibberellins); EC 2.1.1.- (Methyltransferases)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170510
[Lr] Data última revisão:
170510
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160621
[St] Status:MEDLINE
[do] DOI:10.1111/jipb.12486


  9 / 134 MEDLINE  
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[PMID]:27317969
[Au] Autor:Xu H; Cao D; Feng J; Wu H; Lin J; Wang Y
[Ad] Endereço:Key Laboratory for Genetics and Breeding of Forest Trees and Ornamental Plants of Ministry of Education, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China.
[Ti] Título:Transcriptional regulation of vascular cambium activity during the transition from juvenile to mature stages in Cunninghamia lanceolata.
[So] Source:J Plant Physiol;200:7-17, 2016 Aug 01.
[Is] ISSN:1618-1328
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Cunninghamia lanceolata (Lamb.) Hook., an evergreen conifer distributed in southern China, has been recognized as the most commercially important timber species due to its rapid growth. However, the molecular mechanisms underlying growth alternation due to vascular cambium activity are poorly understood. Here, we used cryosectioning to isolate the vascular cambium tissue of C. lanceolata at three stages, namely, juvenile, transition and mature (3-, 13-, and 35-year-old trees respectively) for transcriptome-wide analysis. Through assembling and annotation of transcripts, 108,767 unigenes and some potential growth-regulated genes were identified. A total of 5213, 4873 and 2541 differentially expressed genes (DEGs) were identified in the three stages. DEGs related to cambial activity, cell division and cell wall modification were detected at various developmental stages of the vascular cambium. In addition, some putative genes involved in plant hormone biosynthesis were also differentially regulated. These results indicate that various cambium-related molecular activities result in alterations in the growth of C. lanceolata, particularly during the transition from juvenile to mature stages. The findings of the present study improve our understanding of cambium development and may aid in studies of the molecular mechanisms of wood production and provide fundamental insights into the establishment of the optimal rotation period for silvicultural trees.
[Mh] Termos MeSH primário: Câmbio/genética
Cunninghamia/crescimento & desenvolvimento
Cunninghamia/genética
Perfilação da Expressão Gênica
Regulação da Expressão Gênica de Plantas
Feixe Vascular de Plantas/genética
[Mh] Termos MeSH secundário: Cunninghamia/anatomia & histologia
Regulação da Expressão Gênica no Desenvolvimento
Ontologia Genética
Anotação de Sequência Molecular
Reação em Cadeia da Polimerase em Tempo Real
Reprodutibilidade dos Testes
Análise de Sequência de RNA
Transdução de Sinais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170410
[Lr] Data última revisão:
170410
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160619
[St] Status:MEDLINE


  10 / 134 MEDLINE  
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[PMID]:27111502
[Au] Autor:Ding Q; Zeng J; He XQ
[Ad] Endereço:State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, China.
[Ti] Título:MiR169 and its target PagHAP2-6 regulated by ABA are involved in poplar cambium dormancy.
[So] Source:J Plant Physiol;198:1-9, 2016 Jul 01.
[Is] ISSN:1618-1328
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Dormancy is an effective strategy for perennial plants in temperate zones to survive the winter stress. MicroRNAs (miRNAs) have been well known as important regulators for various biological processes. In this study, we checked the expression of miR169 members in the cambium zone during dormancy and active growth in poplar and found that they had distinct expression patterns. We identified and characterized a dormancy-specific target gene of miR169, PagHAP2-6. 5' RACE assays confirmed the direct cleavage of PagHAP2-6 mRNA by miR169. The yeast functional complementation analysis showed that PagHAP2-6 was a homolog of Heme Activator Protein2 (HAP2)/Nuclear factor Y-A (NF-YA) transcription factor in poplar. qRT-PCR analysis indicated that PagHAP2-6 was highly expressed in the dormant stage, which was converse to the expression pattern of pag-miR169a, n, and r. In addition, the transcription of PagHAP2-6 was induced by exogenous abscisic acid (ABA), and both over-expression of PagHAP2-6 in Arabidopsis and transient co-expression assays in Nicotiana benthamiana indicated that PagHAP2-6 could increase the resistance to exogenous ABA. Taken together, the results suggested that miR169 and its target PagHAP2-6 regulated by ABA were involved in poplar cambium dormancy, which provided new insights into the regulatory mechanisms of tree dormancy-active growth transition.
[Mh] Termos MeSH primário: Ácido Abscísico/farmacologia
Câmbio/genética
Genes de Plantas
MicroRNAs/metabolismo
Dormência de Plantas/genética
Populus/genética
[Mh] Termos MeSH secundário: Sequência de Bases
Câmbio/efeitos dos fármacos
Clonagem Molecular
Regulação da Expressão Gênica de Plantas/efeitos dos fármacos
Teste de Complementação Genética
MicroRNAs/genética
Dormência de Plantas/efeitos dos fármacos
Folhas de Planta/efeitos dos fármacos
Folhas de Planta/genética
Folhas de Planta/crescimento & desenvolvimento
Proteínas de Plantas/química
Proteínas de Plantas/genética
Proteínas de Plantas/metabolismo
Populus/efeitos dos fármacos
Saccharomyces cerevisiae/metabolismo
Transcrição Genética/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (MicroRNAs); 0 (Plant Proteins); 72S9A8J5GW (Abscisic Acid)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170410
[Lr] Data última revisão:
170410
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160426
[St] Status:MEDLINE



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