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[PMID]: 29360842 [Au] Autor: Liu F; Yang Y; Gao J; Ma C; Bi Y [Ad] Endereço: College of Life Science, Shandong Normal University, Jinan, China. [Ti] Título: A comparative transcriptome analysis of a wild purple potato and its red mutant provides insight into the mechanism of anthocyanin transformation. [So] Source: PLoS One;13(1):e0191406, 2018. [Is] ISSN: 1932-6203 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: In this study, a red mutant was obtained through in vitro regeneration of a wild purple potato. High-performance liquid chromatography and Mass spectrometry analysis revealed that pelargonidin-3-O-glucoside and petunidin-3-O-glucoside were main anthocyanins in the mutant and wild type tubers, respectively. In order to thoroughly understand the mechanism of anthocyanin transformation in two materials, a comparative transcriptome analysis of the mutant and wild type was carried out through high-throughput RNA sequencing, and 295 differentially expressed genes (DEGs) were obtained. Real-time qRT-PCR validation of DEGs was consistent with the transcriptome date. The DEGs mainly influenced biological and metabolic pathways, including phenylpropanoid biosynthesis and translation, and biosynthesis of flavone and flavonol. In anthocyanin biosynthetic pathway, the analysis of structural genes expressions showed that three genes, one encoding phenylalanine ammonia-lyase, one encoding 4-coumarate-CoA ligase and one encoding flavonoid 3',5'-hydroxylasem were significantly down-regulated in the mutant; one gene encoding phenylalanine ammonia-lyase was significantly up-regulated. Moreover, the transcription factors, such as bZIP family, MYB family, LOB family, MADS family, zf-HD family and C2H2 family, were significantly regulated in anthocyanin transformation. Response proteins of hormone, such as gibberellin, abscisic acid and brassinosteroid, were also significantly regulated in anthocyanin transformation. The information contributes to discovering the candidate genes in anthocyanin transformation, which can serve as a comprehensive resource for molecular mechanism research of anthocyanin transformation in potatoes. [Mh] Termos MeSH primário: Antocianinas/biossíntese Antocianinas/genética Solanum tuberosum/genética Solanum tuberosum/metabolismo [Mh] Termos MeSH secundário: Vias Biossintéticas/genética Coenzima A Ligases/genética Sistema Enzimático do Citocromo P-450/genética Perfilação da Expressão Gênica Regulação da Expressão Gênica de Plantas Genes de Plantas Glucosídeos/biossíntese Glucosídeos/genética Sequenciamento de Nucleotídeos em Larga Escala Mutação Fenilalanina Amônia-Liase/genética Pigmentação/genética Reguladores de Crescimento de Planta/genética Proteínas de Plantas/genética Tubérculos/genética Tubérculos/metabolismo RNA de Plantas/genética Fatores de Transcrição/genética [Pt] Tipo de publicação: COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Anthocyanins); 0 (Glucosides); 0 (Plant Growth Regulators); 0 (Plant Proteins); 0 (RNA, Plant); 0 (Transcription Factors); 6988-81-4 (petunidin-3-glucoside); 8H1WZY9R6P (pelargonidin-3-glucoside); 9035-51-2 (Cytochrome P-450 Enzyme System); EC 1.14.- (flavonoid 3',5'-hydroxylase); EC 4.3.1.24 (Phenylalanine Ammonia-Lyase); EC 6.2.1.- (Coenzyme A Ligases); EC 6.2.1.12 (4-coumarate-CoA ligase) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180227 [Lr] Data última revisão: 180227 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180124 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pone.0191406

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[PMID]: 29328647 [Au] Autor: Hsieh HL; Lee CH; Lin KC [Ad] Endereço: Department of Life Science, National Dong Hwa University , Hualien County 974, Taiwan. [Ti] Título: Development of Yam Dioscorin-Loaded Nanoparticles for Paracellular Transport Across Human Intestinal Caco-2 Cell Monolayers. [So] Source: J Agric Food Chem;66(5):1175-1183, 2018 Feb 07. [Is] ISSN: 1520-5118 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Dioscorins, the major storage proteins of yam tubers, exert immunomodulatory activities. To improve oral bioavailability of dioscorins in the intestine, recombinant dioscorin (rDioscorin) was coated with N,N,N-trimethyl chitosan (TMC) and tripolyphosphate (TPP), resulting in the formation of TMC-rDio-TPP nanoparticles (NPs). The loading capacity and entrapment efficiency of rDioscorin in the NPs were 26 ± 0.7% and 61 ± 1.4%, respectively. The NPs demonstrated a substantial release profile in the pH environment of the jejunum. The rDioscorin released from the NPs stimulated proliferation and phagocytosis of the macrophage RAW264.7 and activated the gene expression of IL-1ß and IL-6. Incubation of the NPs in the Caco-2 cell monolayer led to a 5.2-fold increase of P compared with rDioscorin alone, suggesting that rDioscorin, with the assistance of TMC, can be promptly transported across the intestinal epithelia. These results demonstrate that the TMC-rDio-TPP NPs can be utilized for elucidating the immunopharmacological effects of dioscorins through oral delivery. [Mh] Termos MeSH primário: Dioscorea/química Intestinos/metabolismo Nanopartículas/metabolismo Proteínas de Plantas/metabolismo [Mh] Termos MeSH secundário: Administração Oral Disponibilidade Biológica Transporte Biológico Células CACO-2 Proliferação Celular/efeitos dos fármacos Quitosana Expressão Gênica/efeitos dos fármacos Seres Humanos Concentração de Íons de Hidrogênio Interleucina-1beta/genética Interleucina-6/genética Mucosa Intestinal/metabolismo Nanopartículas/administração & dosagem Fagocitose/efeitos dos fármacos Proteínas de Plantas/administração & dosagem Proteínas de Plantas/farmacocinética Tubérculos/química Polifosfatos [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Interleukin-1beta); 0 (Interleukin-6); 0 (N-trimethyl chitosan chloride); 0 (Plant Proteins); 0 (Polyphosphates); 0 (dioscorin protein, Dioscorea); 9012-76-4 (Chitosan); NU43IAG5BC (triphosphoric acid) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180226 [Lr] Data última revisão: 180226 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180113 [St] Status: MEDLINE [do] DOI: 10.1021/acs.jafc.7b04150

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[PMID]: 28742457 [Au] Autor: Buddenhagen CE; Hernandez Nopsa JF; Andersen KF; Andrade-Piedra J; Forbes GA; Kromann P; Thomas-Sharma S; Useche P; Garrett KA [Ad] Endereço: First, second, third, and ninth authors: Plant Pathology Department, Institute for Sustainable Food Systems, and Emerging Pathogens Institute, University of Florida, Gainesville 32611; fourth and fifth authors: International Potato Center, Lima, Peru; sixth author: International Potato Center, Quito [Ti] Título: Epidemic Network Analysis for Mitigation of Invasive Pathogens in Seed Systems: Potato in Ecuador. [So] Source: Phytopathology;107(10):1209-1218, 2017 10. [Is] ISSN: 0031-949X [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Seed systems have an important role in the distribution of high-quality seed and improved varieties. The structure of seed networks also helps to determine the epidemiological risk for seedborne disease. We present a new approach for evaluating the epidemiological role of nodes in seed networks, and apply it to a regional potato farmer consortium (Consorcio de Productores de Papa [CONPAPA]) in Ecuador. We surveyed farmers to estimate the structure of networks of farmer seed tuber and ware potato transactions, and farmer information sources about pest and disease management. Then, we simulated pathogen spread through seed transaction networks to identify priority nodes for disease detection. The likelihood of pathogen establishment was weighted based on the quality or quantity of information sources about disease management. CONPAPA staff and facilities, a market, and certain farms are priorities for disease management interventions such as training, monitoring, and variety dissemination. Advice from agrochemical store staff was common but assessed as significantly less reliable. Farmer access to information (reported number and quality of sources) was similar for both genders. However, women had a smaller amount of the market share for seed tubers and ware potato. Understanding seed system networks provides input for scenario analyses to evaluate potential system improvements. [Formula: see text] Copyright © 2017 The Author(s). This is an open access article distributed under the CC BY 4.0 International license . [Mh] Termos MeSH primário: Epidemias Espécies Introduzidas Doenças das Plantas/microbiologia Sementes/microbiologia Solanum tuberosum/microbiologia [Mh] Termos MeSH secundário: Simulação por Computador Produtos Agrícolas Equador Feminino Seres Humanos Masculino Modelos Teóricos Doenças das Plantas/estatística & dados numéricos Tubérculos/microbiologia [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S. [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180215 [Lr] Data última revisão: 180215 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170726 [St] Status: MEDLINE [do] DOI: 10.1094/PHYTO-03-17-0108-FI

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[PMID]: 29337064 [Au] Autor: Knollenberg BJ; Liu J; Yu S; Lin H; Tian L [Ad] Endereço: Department of Plant Sciences, University of California, Davis, CA, 95616, USA; The Huck Institutes of the Life Sciences, Pennsylvania State University, State College, PA, 16801, USA. [Ti] Título: Cloning and functional characterization of a p-coumaroyl quinate/shikimate 3'-hydroxylase from potato (Solanum tuberosum). [So] Source: Biochem Biophys Res Commun;496(2):462-467, 2018 02 05. [Is] ISSN: 1090-2104 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Chlorogenic acid (CGA) plays an important role in protecting plants against pathogens and promoting human health. Although CGA accumulates to high levels in potato tubers, the key enzyme p-coumaroyl quinate/shikimate 3'-hydroxylase (C3'H) for CGA biosynthesis has not been isolated and functionally characterized in potato. In this work, we cloned StC3'H from potato and showed that it catalyzed the formation of caffeoylshikimate and CGA (caffeoylquinate) from p-coumaroyl shikimate and p-coumaroyl quinate, respectively, but was inactive towards p-coumaric acid in in vitro enzyme assays. When the expression of StC3'H proteins was blocked through antisense (AS) inhibition under the control of a tuber-specific patatin promoter, moderate changes in tuber yield as well as phenolic metabolites in the core tuber tissue were observed for several AS lines. On the other hand, the AS and control potato lines exhibited similar responses to a bacterial pathogen Pectobacterium carotovorum. These results suggest that StC3'H is implicated in phenolic metabolism in potato. They also suggest that CGA accumulation in the core tissue of potato tubers is an intricately controlled process and that additional C3'H activity may also be involved in CGA biosynthesis in potato. [Mh] Termos MeSH primário: Ácido Clorogênico/metabolismo Oxigenases de Função Mista/genética Proteínas de Plantas/genética Tubérculos/enzimologia Solanum tuberosum/enzimologia [Mh] Termos MeSH secundário: Hidrolases de Éster Carboxílico/genética Hidrolases de Éster Carboxílico/metabolismo Ácido Clorogênico/análogos & derivados Clonagem Molecular Expressão Gênica Oxigenases de Função Mista/antagonistas & inibidores Oxigenases de Função Mista/metabolismo Oligonucleotídeos Antissenso/genética Oligonucleotídeos Antissenso/metabolismo Pectobacterium carotovorum/patogenicidade Pectobacterium carotovorum/fisiologia Pichia/genética Pichia/metabolismo Proteínas de Plantas/metabolismo Tubérculos/genética Tubérculos/microbiologia Plantas Geneticamente Modificadas Proteínas Recombinantes/genética Proteínas Recombinantes/metabolismo Ácido Chiquímico/análogos & derivados Ácido Chiquímico/metabolismo Solanum tuberosum/genética Solanum tuberosum/microbiologia [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S. [Nm] Nome de substância: 0 (4-coumaroylshikimic acid); 0 (Oligonucleotides, Antisense); 0 (Plant Proteins); 0 (Recombinant Proteins); 0 (patatin protein, Solanum tuberosum); 29MS2WI2NU (Shikimic Acid); 318ADP12RI (Chlorogenic Acid); 73263-62-4 (5-O-caffeoylshikimic acid); E57A0DKE0B (3,4-di-O-caffeoylquinic acid); EC 1.- (Mixed Function Oxygenases); EC 1.14.13.- (5-O-(4-coumaroyl)shikimate 3'-hydroxylase); EC 3.1.1.- (Carboxylic Ester Hydrolases) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180213 [Lr] Data última revisão: 180213 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180117 [St] Status: MEDLINE

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[PMID]: 28597466 [Au] Autor: Wiberley-Bradford AE; Bethke PC [Ad] Endereço: Department of Horticulture, University of Wisconsin-Madison, Madison, WI, USA. [Ti] Título: Suppression of the vacuolar invertase gene delays senescent sweetening in chipping potatoes. [So] Source: J Sci Food Agric;98(1):354-360, 2018 Jan. [Is] ISSN: 1097-0010 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: BACKGROUND: Potato chip processors require potato tubers that meet quality specifications for fried chip color, and color depends largely upon tuber sugar contents. At later times in storage, potatoes accumulate sucrose, glucose, and fructose. This developmental process, senescent sweetening, manifests as a blush of color near the center of the fried chip, becomes more severe with time, and limits the storage period. Vacuolar invertase (VInv) converts sucrose to glucose and fructose and is hypothesized to play a role in senescent sweetening. To test this hypothesis, senescent sweetening was quantified in multiple lines of potato with reduced VInv expression. RESULTS: Chip darkening from senescent sweetening was delayed by about 4 weeks for tubers with reduced VInv expression. A strong positive correlation between frequency of dark chips and tuber hexose content was observed. Tubers with reduced VInv expression had lower hexose to sucrose ratios than controls. CONCLUSION: VInv activity contributes to reducing sugar accumulation during senescent sweetening. Sucrose breakdown during frying may contribute to chip darkening. Suppressing VInv expression increases the storage period of the chipping potato crop, which is an important consideration, as potatoes with reduced VInv expression are entering commercial production in the USA. © 2017 Society of Chemical Industry. [Mh] Termos MeSH primário: Aromatizantes/metabolismo Proteínas de Plantas/genética Solanum tuberosum/enzimologia beta-Frutofuranosidase/genética [Mh] Termos MeSH secundário: Culinária Aromatizantes/química Seres Humanos Proteínas de Plantas/química Proteínas de Plantas/metabolismo Tubérculos/química Tubérculos/enzimologia Tubérculos/genética Solanum tuberosum/química Solanum tuberosum/genética Paladar beta-Frutofuranosidase/química beta-Frutofuranosidase/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Flavoring Agents); 0 (Plant Proteins); EC 3.2.1.26 (beta-Fructofuranosidase) [Em] Mês de entrada: 1712 [Cu] Atualização por classe: 171228 [Lr] Data última revisão: 171228 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170610 [St] Status: MEDLINE [do] DOI: 10.1002/jsfa.8478

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[PMID]: 28585268 [Au] Autor: Zhang Z; Guo C; Gao T; Fu H; Chen Q; Wang Y [Ad] Endereço: College of Food Science & Engineering, Northwest A&F University, Yangling, Shaanxi, China. [Ti] Título: Pilot-scale radiofrequency blanching of potato cuboids: heating uniformity. [So] Source: J Sci Food Agric;98(1):312-320, 2018 Jan. [Is] ISSN: 1097-0010 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: BACKGROUND: Traditional hot water blanching has a slow heat transfer rate, whereas radiofrequency (RF) heating has the advantages of a much faster heating rate and a higher penetration depth. In the present study, RF heating was applied to improve heating uniformity for subsequent blanching experiments involving potato cuboids. Potato cuboids were treated in a pilot-scale, RF heating system (27.12 MHz, 6 kW) under different operating conditions. RESULTS: The dielectric constant increased first and then decreased with temperature, whereas the loss factor increased as the temperature increased. The results of the present study reveal that the electrode gap, sample height and NaCl solution had significant effects (P < 0.05) on the temperature distribution and heating uniformity of the sample after RF heating. The optimum RF heating uniformity was obtained at an electrode gap of 120 mm, a sample height of 60 mm and when immersed in NaCl solution of 0.5 s m . The central heating pattern was presented in a sample. Cold spots were located at the edge of the top surface of the sample. CONCLUSION: The present study shows the great potential of RF heating for the blanching of vegetables. Future studies should aim to determine changes in the texture and nutrient contents of vegetables during RF heating. © 2017 Society of Chemical Industry. [Mh] Termos MeSH primário: Tubérculos/efeitos da radiação Solanum tuberosum/efeitos da radiação [Mh] Termos MeSH secundário: Manipulação de Alimentos Temperatura Alta Tubérculos/química Ondas de Rádio Solanum tuberosum/química [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1712 [Cu] Atualização por classe: 171228 [Lr] Data última revisão: 171228 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170607 [St] Status: MEDLINE [do] DOI: 10.1002/jsfa.8473

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[PMID]: 28873636 [Au] Autor: Romano A; Masi P; Aversano R; Carucci F; Palomba S; Carputo D [Ad] Endereço: Centre for Food Innovation and Development in the Food Industry, University of Naples Federico II, Via Università 100, 80055, Portici, Naples, Italy. Electronic address: annalisa.romano@unina.it. [Ti] Título: Microstructure and tuber properties of potato varieties with different genetic profiles. [So] Source: Food Chem;239:789-796, 2018 Jan 15. [Is] ISSN: 0308-8146 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: The objectives of this research were to study tuber starch characteristics and chemical - thermal properties of 21 potato varieties, and to determine their genetic diversity through SSR markers. Starch granular size varied among samples, with a wide diameter distribution (5-85µm), while granule shapes were similar. Differential Scanning Calorimeter analysis showed that the transition temperatures (69°C-74°C) and enthalpies of gelatinization (0.9J/g-3.8J/g) of tubers were also variety dependent. SSR analysis allowed the detection of 157 alleles across all varieties, with an average value of 6.8 alleles per locus. Variety-specific alleles were also identified. SSR-based cluster analysis revealed that varieties with interesting quality attributes were distributed among all clusters and sub-clusters, suggesting that the genetic basis of traits analyzed may differ among our varieties. The information obtained in this study may be useful to identify and develop varieties with slowly digestible starch. [Mh] Termos MeSH primário: Solanum tuberosum [Mh] Termos MeSH secundário: Alelos Fenótipo Tubérculos Amido [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 9005-25-8 (Starch) [Em] Mês de entrada: 1711 [Cu] Atualização por classe: 171128 [Lr] Data última revisão: 171128 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170907 [St] Status: MEDLINE

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[PMID]: 28867090 [Au] Autor: Judprasong K; Archeepsudcharit N; Chantapiriyapoon K; Tanaviyutpakdee P; Temviriyanukul P [Ad] Endereço: Institute of Nutrition, Mahidol University, Putthamonthon 4 Rd., Salaya, Phutthamonthon, Nakhon Pathom 73170, Thailand. Electronic address: Kunchit.jud@mahidol.ac.th. [Ti] Título: Nutrients and natural toxic substances in commonly consumed Jerusalem artichoke (Helianthus tuberosus L.) tuber. [So] Source: Food Chem;238:173-179, 2018 Jan 01. [Is] ISSN: 0308-8146 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: This study determined nutrients, chemical contaminants, (insecticide residues and heavy metals), and natural toxic substances (nitrate, nitrite, cyanide, oxalate, phytate, and trypsin inhibitor) in tubers of Jerusalem artichokes-Kaentawan in the Thai language-grown in four major provinces in Thailand. They were purchased, prepared, homogenized, and freeze-dried for further analysis using standard methods. All Kaentawan samples contained considerable amounts of fructans and dietary fiber (15.4±0.2gand3.2±0.8g/100gfresh weight [FW], respectively), as well as potassium and iron (339±61and0.32±0.05mg/100gFW, respectively). All samples had very low amounts of insecticide residues (37 compounds), cyanide, and trypsin inhibitor, as well as Pb, Cd, nitrate, and nitrite (0.82±0.09, 0.10±0.02, 1.9-17.5, and 0.01-0.24mgkg FW, respectively), in addition to oxalate and phytate (14±9and0.17±0.02mg/100gFW, respectively). This study's data can be used for food composition databases and for safety consumption. [Mh] Termos MeSH primário: Helianthus [Mh] Termos MeSH secundário: Alimentos Frutanos Tubérculos [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Fructans) [Em] Mês de entrada: 1711 [Cu] Atualização por classe: 171128 [Lr] Data última revisão: 171128 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170905 [St] Status: MEDLINE

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[PMID]: 28898255 [Au] Autor: Sulli M; Mandolino G; Sturaro M; Onofri C; Diretto G; Parisi B; Giuliano G [Ad] Endereço: ENEA, Casaccia Research Center, Via Anguillarese 301, Roma, Italy. [Ti] Título: Molecular and biochemical characterization of a potato collection with contrasting tuber carotenoid content. [So] Source: PLoS One;12(9):e0184143, 2017. [Is] ISSN: 1932-6203 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: After wheat and rice, potato is the third most important staple food worldwide. A collection of ten tetraploid (Solanum tuberosum) and diploid (S. phureja and S. chacoense) genotypes with contrasting carotenoid content was subjected to molecular characterization with respect to candidate carotenoid loci and metabolic profiling using LC-HRMS. Irrespective of ploidy and taxonomy, tubers of these genotypes fell into three groups: yellow-fleshed, characterized by high levels of epoxy-xanthophylls and xanthophyll esters and by the presence of at least one copy of a dominant allele of the ß-Carotene Hydroxylase 2 (CHY2) gene; white-fleshed, characterized by low carotenoid levels and by the presence of recessive chy2 alleles; and orange-fleshed, characterized by high levels of zeaxanthin but low levels of xanthophyll esters, and homozygosity for a Zeaxanthin Epoxidase (ZEP) recessive allele. Novel CHY2 and ZEP alleles were identified in the collection. Multivariate analysis identified several groups of co-regulated non-polar compounds, and resulted in the grouping of the genotypes according to flesh color, suggesting that extensive cross-talk exists between the carotenoid pathway and other metabolite pathways in tubers. Postharvest traits like tuber dormancy and weight loss during storage showed little correlation with tuber carotenoid content, with the exception of zeaxanthin and its esters. Other tuber metabolites, such as glucose, monogalactosyldiacyglycerol (a glycolipid), or suberin precursors, showed instead significant correlations with both traits. [Mh] Termos MeSH primário: Carotenoides/metabolismo Tubérculos/metabolismo Solanum tuberosum/genética Solanum tuberosum/metabolismo [Mh] Termos MeSH secundário: Alelos Carotenoides/análise Análise por Conglomerados Diploide Perfilação da Expressão Gênica Regulação da Expressão Gênica de Plantas Redes Reguladoras de Genes Genes de Plantas Estudos de Associação Genética Genótipo Metaboloma Metabolômica/métodos Fenótipo Locos de Características Quantitativas Tetraploidia Xantofilas/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Xanthophylls); 36-88-4 (Carotenoids) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171018 [Lr] Data última revisão: 171018 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170913 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pone.0184143

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[PMID]: 28867709 [Au] Autor: Kim YJ; Lim HS; Kim Y; Lee J; Kim BY; Jeong SJ [Ad] Endereço: Herbal Medicine Research Division, Korea Institute of Oriental Medicine. [Ti] Título: Neuroprotective Effect of Corydalis ternata Extract and Its Phytochemical Quantitative Analysis. [So] Source: Chem Pharm Bull (Tokyo);65(9):826-832, 2017. [Is] ISSN: 1347-5223 [Cp] País de publicação: Japan [La] Idioma: eng [Ab] Resumo: The tubers of Corydalis ternata have been used to treat cardiovascular diseases such as hypertension and cardiac arrhythmia. Its active components have anticholinesterase, antiamnesic, and anti-inflammatory activities, and analgesic effects. In the present study, we performed quantitative analyses of the two components of C. ternata, coptisine and berberine, using HPLC. A 70% ethanol extract of C. ternata was prepared and the two components were separated using a C-18 analytical column on a gradient solvent system of acetonitrile and 0.1% (v/v) aqueous trifluoroacetic acid. Recordings were performed at a UV wavelength of 265 nm for two standard components. The established analytical method showed high linearity (correlation coefficient (r)=1.0000) and proper precision (0.49-3.88%), accuracy (97.88-102.7%), and recovery (95.12-103.79%) for two standard components. The amount of the coptisine and berberine was 4.968±0.089 mg/g and 3.73±0.075 mg/g, respectively. In addition, we investigated the effects of coptisine and berberine on acetylcholinesterase activity and amyloid-ß aggregation, which are major biomarkers of dementia. Coptisine and berberine decreased acetylcholinesterase activity in a dose-dependent manner (IC =0.74 and 0.48 µM, respectively). The C. ternata extract exerted an antioxidant activity by stimulating the radical scavenging activity of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), but not 2,2-diphenyl-1-picrylhydrazyl (DPPH). Furthermore, the C. ternata extract reversed the hydrogen peroxide-induced death of HT22 hippocampal cells, indicating its neuroprotective effect. Our results suggest the potential of C. ternata as a therapeutic agent against dementia via the inhibition of acetylcholinesterase activity and neuronal cell death. [Mh] Termos MeSH primário: Corydalis/química Fármacos Neuroprotetores/química Fármacos Neuroprotetores/farmacologia Estresse Oxidativo/efeitos dos fármacos Extratos Vegetais/farmacologia [Mh] Termos MeSH secundário: Acetilcolinesterase/química Acetilcolinesterase/metabolismo Berberina/análogos & derivados Berberina/química Berberina/isolamento & purificação Linhagem Celular Tumoral Sobrevivência Celular/efeitos dos fármacos Cromatografia Líquida de Alta Pressão Corydalis/metabolismo Depuradores de Radicais Livres/química Depuradores de Radicais Livres/isolamento & purificação Seres Humanos Peróxido de Hidrogênio/toxicidade Fármacos Neuroprotetores/isolamento & purificação Extratos Vegetais/química Tubérculos/química Tubérculos/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Free Radical Scavengers); 0 (Neuroprotective Agents); 0 (Plant Extracts); 0GCL71VN14 (coptisine); 0I8Y3P32UF (Berberine); BBX060AN9V (Hydrogen Peroxide); EC 3.1.1.7 (Acetylcholinesterase) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171019 [Lr] Data última revisão: 171019 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170905 [St] Status: MEDLINE [do] DOI: 10.1248/cpb.c17-00300

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