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  1 / 1134 MEDLINE  
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[PMID]:28461402
[Au] Autor:Lakhssassi N; Colantonio V; Flowers ND; Zhou Z; Henry J; Liu S; Meksem K
[Ad] Endereço:Department of Plant, Soil, and Agricultural Systems, Southern Illinois University, Carbondale, Illinois 62901.
[Ti] Título:Stearoyl-Acyl Carrier Protein Desaturase Mutations Uncover an Impact of Stearic Acid in Leaf and Nodule Structure.
[So] Source:Plant Physiol;174(3):1531-1543, 2017 Jul.
[Is] ISSN:1532-2548
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Stearoyl-acyl carrier protein desaturase (SACPD-C) has been reported to control the accumulation of seed stearic acid; however, no study has previously reported its involvement in leaf stearic acid content and impact on leaf structure and morphology. A subset of an ethyl methanesulfonate mutagenized population of soybean ( ) 'Forrest' was screened to identify mutants within the gene. Using a forward genetics approach, one nonsense and four missense mutants were identified to have high levels of seed, nodule, and leaf stearic acid content. Homology modeling and in silico analysis of the GmSACPD-C enzyme revealed that most of these mutations were localized near or at conserved residues essential for diiron ion coordination. Soybeans carrying mutations at conserved residues showed the highest stearic acid content, and these mutations were found to have deleterious effects on nodule development and function. Interestingly, mutations at nonconserved residues show an increase in stearic acid content yet retain healthy nodules. Thus, random mutagenesis and mutational analysis allows for the achievement of high seed stearic acid content with no associated negative agronomic characteristics. Additionally, expression analysis demonstrates that nodule leghemoglobin transcripts were significantly more abundant in soybeans with deleterious mutations at conserved residues of GmSACPD-C. Finally, we report that mutations cause an increase in leaf stearic acid content and an alteration of leaf structure and morphology in addition to differences in nitrogen-fixing nodule structure.
[Mh] Termos MeSH primário: Oxigenases de Função Mista/metabolismo
Mutação/genética
Folhas de Planta/metabolismo
Proteínas de Plantas/metabolismo
Nódulos Radiculares de Plantas/metabolismo
Feijão de Soja/enzimologia
Ácidos Esteáricos/metabolismo
[Mh] Termos MeSH secundário: Alelos
Sequência de Aminoácidos
Sequência de Bases
Sequência Conservada
Análise Mutacional de DNA
Regulação da Expressão Gênica de Plantas
Testes Genéticos
Leghemoglobina/metabolismo
Oxigenases de Função Mista/química
Oxigenases de Função Mista/genética
Modelos Moleculares
Folhas de Planta/anatomia & histologia
Proteínas de Plantas/química
Proteínas de Plantas/genética
Sementes/metabolismo
Feijão de Soja/genética
Homologia Estrutural de Proteína
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Leghemoglobin); 0 (Plant Proteins); 0 (Stearic Acids); 4ELV7Z65AP (stearic acid); EC 1.- (Mixed Function Oxygenases); EC 1.14.19.2 (acyl-(acyl-carrier-protein)desaturase)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180115
[Lr] Data última revisão:
180115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1104/pp.16.01929


  2 / 1134 MEDLINE  
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[PMID]:28945783
[Au] Autor:Puozaa DK; Jaiswal SK; Dakora FD
[Ad] Endereço:Department of Crop Sciences, Tshwane, University of Technology, Pretoria, South Africa.
[Ti] Título:African origin of Bradyrhizobium populations nodulating Bambara groundnut (Vigna subterranea L. Verdc) in Ghanaian and South African soils.
[So] Source:PLoS One;12(9):e0184943, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Flavonoids secreted by legumes play a major role as signal molecules for attracting compatible rhizobia. The aim of this study was to assess and understand the diversity of microsymbionts nodulating Bambara groundnut (Vigna subterranea L. Verdc.) landraces of different seedcoat colours using restriction fragment length polymorphism and phylogenetic analysis. Seedcoat pigmentation of landraces had effect on the diversity of microsymbionts of Bambara groundnut. Even when planted together in one hole, nodulating bradyrhizobia clustered differently. For example, 16S rDNA-RFLP typing of rhizobial samples TUTVSBLM.I, TUTVSCRM.I and TUTVSRDM.I originating respectively from Black, Cream and Red landraces that were co-planted in the same hole at Manga in the Sudano-sahelian savanna, as well as TUTVSCRK.I and TUTVSRDK.I respectively from Cream and Red landraces co-planted at Kpalisogu in the Guinea savanna, revealed different 16S rDNA- RFLP types. Phylogenetic analysis of 16S rDNA, glnII, recA and atpD sequences showed that Vigna subterranea was nodulated specifically by a diverse group of Bradyrhizobium species (e.g. Bradyrhizobium vignae, and a novel group of Bradyrhizobium spp.) in soils from Ghana and South Africa. The recA gene phylogeny showed incongruency with the other housekeeping genes, indicating the possibility of lateral gene transfer and/or recombination events. The grouping of isolates according to symbiotic gene (nifH and nodD) phylogenies revealed inter- and intra-specific symbiotic plasmid transfer and different evolutionary history. The results also showed that a cropping history and physico-chemical environment of soils increased bradyrhizobial diversity in Ghana and South Africa.
[Mh] Termos MeSH primário: Bradyrhizobium
Nódulos Radiculares de Plantas/microbiologia
Microbiologia do Solo
Vigna/microbiologia
[Mh] Termos MeSH secundário: Bradyrhizobium/genética
Bradyrhizobium/fisiologia
Gana
Filogenia
Polimorfismo de Fragmento de Restrição/genética
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
África do Sul
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170926
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184943


  3 / 1134 MEDLINE  
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[PMID]:28700717
[Au] Autor:Ogden AJ; Gargouri M; Park J; Gang DR; Kahn ML
[Ad] Endereço:Molecular Plant Science Program, Washington State University, Pullman, Washington, United States of America.
[Ti] Título:Integrated analysis of zone-specific protein and metabolite profiles within nitrogen-fixing Medicago truncatula-Sinorhizobium medicae nodules.
[So] Source:PLoS One;12(7):e0180894, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Symbiotic nitrogen fixation (SNF) between rhizobia and legumes requires metabolic coordination within specialized root organs called nodules. Nodules formed in the symbiosis between S. medicae and barrel medic (M. truncatula) are indeterminate, cylindrical, and contain spatially distinct developmental zones. Bacteria in the infection zone II (ZII), interzone II-III (IZ), and nitrogen fixation zone III (ZIII) represent different stages in the metabolic progression from free-living bacteria into nitrogen fixing bacteroids. To better understand the coordination of plant and bacterial metabolism within the nodule, we used liquid and gas chromatography coupled to tandem mass spectrometry (MS) to observe protein and metabolite profiles representative of ZII, IZ, ZIII, whole-nodule, and primary root. Our MS-based approach confidently identified 361 S. medicae proteins and 888 M. truncatula proteins, as well as 160 metabolites from each tissue. The data are consistent with several organ- and zone-specific protein and metabolite localization patterns characterized previously. We used our comprehensive dataset to demonstrate how multiple branches of primary metabolism are coordinated between symbionts and zones, including central carbon, fatty acid, and amino acid metabolism. For example, M. truncatula glycolysis enzymes accumulate from zone I to zone III within the nodule, while equivalent S. medicae enzymes decrease in abundance. We also show the localization of S. medicae's transition to dicarboxylic acid-dependent carbon metabolism within the IZ. The spatial abundance patterns of S. medicae fatty acid (FA) biosynthesis enzymes indicate an increased demand for FA production in the IZ and ZIII as compared to ZI. These observations provide a resource for those seeking to understand coordinated physiological changes during the development of SNF.
[Mh] Termos MeSH primário: Medicago truncatula/metabolismo
Nitrogênio/metabolismo
Raízes de Plantas/microbiologia
[Mh] Termos MeSH secundário: Ácidos Graxos/metabolismo
Regulação da Expressão Gênica de Plantas/fisiologia
Fixação de Nitrogênio/fisiologia
Nódulos Radiculares de Plantas
Sinorhizobium/metabolismo
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fatty Acids); N762921K75 (Nitrogen)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171014
[Lr] Data última revisão:
171014
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170713
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0180894


  4 / 1134 MEDLINE  
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[PMID]:28693668
[Au] Autor:Marcos-García M; Menéndez E; Ramírez-Bahena MH; Mateos PF; Peix Á; Velazquez E; Rivas R
[Ad] Endereço:1​Departamento de Microbiología y Genética and Centro Hispanoluso de Investigaciones Agrarias (CIALE), Universidad de Salamanca, Salamanca, Spain.
[Ti] Título:Mesorhizobium helmanticense sp. nov., isolated from Lotus corniculatus nodules.
[So] Source:Int J Syst Evol Microbiol;67(7):2301-2305, 2017 Jul.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In this study, three strains belonging to the genus Mesorhizobium, CSLC115NT, CSLC19N and CSLC37N, isolated from Lotus corniculatus nodules in Spain, were characterized. Their 16S rRNA gene sequences were closely related to those of Mesorhizobium metallidurans STM 2683T, Mesorhizobium tianshanense A-1BST, Mesorhizobium tarimense CCBAU 83306T, Mesorhizobium gobiense CCBAU 83330T and Mesorhizobium caraganae CCBAU 11299T with similarity values higher than 99.7 %. The analysis of concatenated recA and glnII genes showed that the most closely related type strains were M. metallidurans STM 2683T, M. tianshanense A-1BST and M. tarimense CCBAU 83306T with 96, 95 and 94 % similarity values in the recA gene and 95, 94 and 94 % in the glnII gene, respectively. M. metallidurans LMG 24485T, M. tianshanense USDA 3592T and M. tarimense LMG 24338T showed means of 44, 41 and 42 % DNA-DNA relatedness, respectively, with respect to strain CSLC115NT. The major fatty acids were those from summed feature 8 (C18 : 1ω7c/C18 : 1ω6c), C16 : 0 and C18 : 1ω7c 11-methyl. The results of phenotypic characterization support that the L. corniculatus nodulating strains analysed in this work belong to a novel species of the genus Mesorhizobium for which the name Mesorhizobium helmanticense sp. nov. is proposed, and the type strain is CSLC115NT (= LMG 29734T=CECT 9168T).
[Mh] Termos MeSH primário: Loteae/microbiologia
Mesorhizobium/classificação
Filogenia
Nódulos Radiculares de Plantas/microbiologia
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Mesorhizobium/genética
Mesorhizobium/isolamento & purificação
Hibridização de Ácido Nucleico
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Espanha
Simbiose
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170913
[Lr] Data última revisão:
170913
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170712
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001942


  5 / 1134 MEDLINE  
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[PMID]:28671523
[Au] Autor:Araújo J; Flores-Félix JD; Igual JM; Peix A; González-Andrés F; Díaz-Alcántara CA; Velázquez E
[Ad] Endereço:1​Facultad de Ciencias Agronómicas y Veterinarias, Universidad Autónoma de Santo Domingo, Santo Domingo, Dominican Republic.
[Ti] Título:Bradyrhizobium cajani sp. nov. isolated from nodules of Cajanus cajan.
[So] Source:Int J Syst Evol Microbiol;67(7):2236-2241, 2017 Jul.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Two slow-growing strains, AMBPC1010T and AMBPC1011, were isolated from nodules of Cajanus cajan in the Dominican Republic. 16S rRNA gene analysis placed these strains within the genus Bradyrhizobium, being phylogenetically equidistant to several species of this genus. Analysis of the recA and atpD genes showed that the strains isolated belong to a cluster containing the strains Bradyrhizobium ottawaense OO99T, 'Bradyrhizobium americanum' CMVU44 and Bradyrhizobium daqingense CCBAU 15774T, and presented similarity values lower than 96 % for both genes with respect to the strains nodulating C. cajan. DNA-DNA hybridization analysis showed averages of 36, 40 and 39 % relatedness with respect to the representative strains of Bradyrhizobium ottawaense, 'Bradyrhizobium americanum' and Bradyrhizobium daqingense, respectively. Phenotypic characteristics also differed from those of the most closely related species of the genus Bradyrhizobium. Therefore, based on the data obtained in this study, we propose to classify the strains AMBPC1010T (=LMG 29967T=CECT 9227T) and AMBPC1011 into a novel species named Bradyrhizobium cajani sp. nov.
[Mh] Termos MeSH primário: Bradyrhizobium/classificação
Cajanus/microbiologia
Filogenia
Nódulos Radiculares de Plantas/microbiologia
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
Bradyrhizobium/genética
Bradyrhizobium/isolamento & purificação
DNA Bacteriano/genética
República Dominicana
Genes Bacterianos
Hibridização de Ácido Nucleico
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Simbiose
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170911
[Lr] Data última revisão:
170911
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170704
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001932


  6 / 1134 MEDLINE  
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[PMID]:28639930
[Au] Autor:Helene LCF; Delamuta JRM; Ribeiro RA; Hungria M
[Ad] Endereço:3​Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, SBN, Quadra 2, Bloco L, Lote 06, Edifício Capes, 70.040-020 Brasília, Distrito Federal, Brazil 2​Department of Microbiology, Universidade Estadual de Londrina, C.P. 10011, 86057-970 Londrina, Paraná, Brazil 1​Embrapa Soja, C.P. 231
[Ti] Título:Bradyrhizobium mercantei sp. nov., a nitrogen-fixing symbiont isolated from nodules of Deguelia costata (syn. Lonchocarpus costatus).
[So] Source:Int J Syst Evol Microbiol;67(6):1827-1834, 2017 Jun.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Some bacteria collectively known as rhizobia can establish symbiotic relationships and the N2-fixation process with several legumes used as green manure, in pastures and for wood production. Symbionts belonging to the genus Bradyrhizobium are predominant in the tropics, and an increasing number of studies report high genetic diversity within the genus. We performed a polyphasic study with two strains belonging to the genus Bradyrhizobium- SEMIA 6399T and SEMIA 6404-isolated from root nodules of Deguelia costata (syn. Lonchocarpus costatus), an important legume native to eastern Brazil. In general, sequences of the 16S rRNA gene were highly conserved in members of the genus Bradyrhizobium, and the two strains were positioned in the Bradyrhizobiumelkanii superclade, sharing 100 % nucleotide identity with Bradyrhizobiumembrapense, Bradyrhizobiumerythrophlei and Bradyrhizobiumviridifuturi. However, multilocus sequence analysis with four housekeeping genes (dnaK, glnII, gyrB and recA) confirmed that the two strains belong to a distinct clade, sharing from 87.7 to 96.1 % nucleotide identity with related species of the genus Bradyrhizobium, being most closely related to B. viridifuturi. Average nucleotide identity of genome sequences between SEMIA 6399T and related species was lower than 92 %, below the threshold of species circumscription. nifH phylogeny clustered the SEMIA strains in a clade separated from other species of the genus Bradyrhizobium, and the nodD phylogeny revealed that SEMIA 6399T presents a more divergent sequence. Other phenotypic and genotypic traits were determined for the new group, and our data support the description of the SEMIA strains as representatives of Bradyrhizobium mercantei sp. nov.; SEMIA 6399T (=CNPSo 1165T=BR 6010T=U675T=LMG 30031T) was chosen as the type strain.
[Mh] Termos MeSH primário: Bradyrhizobium/classificação
Fabaceae/microbiologia
Filogenia
Nódulos Radiculares de Plantas/microbiologia
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
Bradyrhizobium/genética
Bradyrhizobium/isolamento & purificação
Brasil
DNA Bacteriano/genética
Genes Bacterianos
Tipagem de Sequências Multilocus
Fixação de Nitrogênio
Hibridização de Ácido Nucleico
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170623
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001870


  7 / 1134 MEDLINE  
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[PMID]:28629499
[Au] Autor:Yan J; Li Y; Yan H; Chen WF; Zhang X; Wang ET; Han XZ; Xie ZH
[Ad] Endereço:1​Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, 150081, PR China.
[Ti] Título:Agrobacterium salinitolerans sp. nov., a saline-alkaline-tolerant bacterium isolated from root nodule of Sesbania cannabina.
[So] Source:Int J Syst Evol Microbiol;67(6):1906-1911, 2017 Jun.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Two Gram-staining-negative, aerobic bacteria (YIC 5082T and YIC4104) isolated from root nodules of Sesbania cannabina grown in a high-salt and alkaline environment were identified as a group in the genus Agrobacterium because they shared 100 and 99.7 % sequence similarities of 16S rRNA and recA+atpD genes, respectively. These two strains showed 99.2/100 % and 93.9/95.4 % 16S rRNA and recA+atpD gene sequence similarities to Agrobacterium radiobacter LMG140T and Agrobacterium. pusense NRCPB10T, respectively. The average nucleotide identities (ANI) of genome sequences were 89.95 % or lower between YIC 5082T and the species of the genus Agrobacterium examined. Moreover, these two test strains formed a unique nifH lineage deeply separated from other rhizobia. Although the nodC gene was not detected in YIC 5082T and YIC4104, they could form effective root nodules on S. cannabina plants. The main cellular fatty acids in YIC 5082T were summed feature 8 (C18 : 1ω7c/C18 : 1ω6c), C19 : 0cyclo ω8c, summed feature 2 (C12 : 0 aldehyde/unknown equivalent chain length 10.9525) and C16 : 0. The DNA G+C content of YIC 5082T was 59.3 mol%. The failure to utilize d-sorbitol as a carbon source distinguished YIC 5082T from the type strains of related species. YIC 5082T could grow in presence of 5.0 % (w/v) NaCl and at a pH of up to 10.0. Based on results regarding the genetic and phenotypic properties of YIC 5082T and YIC4104 the name Agrobacterium salinitolerans sp. nov. is proposed and YIC 5082T (=HAMBI 3646T=LMG 29287T) is designed as the type strain.
[Mh] Termos MeSH primário: Agrobacterium/classificação
Filogenia
Nódulos Radiculares de Plantas/microbiologia
Sesbania/microbiologia
[Mh] Termos MeSH secundário: Agrobacterium/genética
Agrobacterium/isolamento & purificação
Técnicas de Tipagem Bacteriana
Composição de Bases
China
DNA Bacteriano/genética
Ácidos Graxos/química
Genes Bacterianos
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170825
[Lr] Data última revisão:
170825
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170621
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001885


  8 / 1134 MEDLINE  
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Rey, Luís
Texto completo
[PMID]:28505340
[Au] Autor:Msaddak A; Rejili M; Durán D; Rey L; Imperial J; Palacios JM; Ruiz-Argüeso T; Mars M
[Ad] Endereço:Research Unit Biodiversity and Valorization of Arid Areas Bioresources (BVBAA)-Faculty of Sciences of Gabès, Erriadh, Zrig 6072, Tunisia.
[Ti] Título:Members of Microvirga and Bradyrhizobium genera are native endosymbiotic bacteria nodulating Lupinus luteus in Northern Tunisian soils.
[So] Source:FEMS Microbiol Ecol;93(6), 2017 Jun 01.
[Is] ISSN:1574-6941
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The genetic diversity of bacterial populations nodulating Lupinus luteus (yellow lupine) in Northern Tunisia was examined. Phylogenetic analyses of 43 isolates based on recA and gyrB partial sequences grouped them in three clusters, two of which belong to genus Bradyrhizobium (41 isolates) and one, remarkably, to Microvirga (2 isolates), a genus never previously described as microsymbiont of this lupine species. Representatives of the three clusters were analysed in-depth by multilocus sequence analysis of five housekeeping genes (rrs, recA, glnII, gyrB and dnaK). Surprisingly, the Bradyrhizobium cluster with the two isolates LluI4 and LluTb2 may constitute a new species defined by a separate position between Bradyrhizobium manausense and B. denitrificans. A nodC-based phylogeny identified only two groups: one formed by Bradyrhizobium strains included in the symbiovar genistearum and the other by the Microvirga strains. Symbiotic behaviour of representative isolates was tested, and among the seven legumes inoculated only a difference was observed i.e. the Bradyrhizobium strains nodulated Ornithopus compressus unlike the two strains of Microvirga. On the basis of these data, we conclude that L. luteus root nodule symbionts in Northern Tunisia are mostly strains within the B. canariense/B. lupini lineages, and the remaining strains belong to two groups not previously identified as L. luteus endosymbionts: one corresponding to a new clade of Bradyrhizobium and the other to the genus Microvirga.
[Mh] Termos MeSH primário: Bradyrhizobium/genética
Lupinus/microbiologia
Methylobacteriaceae/genética
Nódulos Radiculares de Plantas/microbiologia
Simbiose/fisiologia
[Mh] Termos MeSH secundário: Proteínas de Bactérias/genética
Bradyrhizobium/classificação
Bradyrhizobium/isolamento & purificação
DNA Girase/genética
DNA Bacteriano/genética
Genes Essenciais/genética
Methylobacteriaceae/classificação
Methylobacteriaceae/isolamento & purificação
Tipagem de Sequências Multilocus
Filogenia
Recombinases Rec A/genética
Análise de Sequência de DNA
Solo
Microbiologia do Solo
Simbiose/genética
Tunísia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (DNA, Bacterial); 0 (NodD protein, Bacteria); 0 (Soil); EC 2.7.7.- (Rec A Recombinases); EC 5.99.1.3 (DNA Gyrase)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170516
[St] Status:MEDLINE
[do] DOI:10.1093/femsec/fix068


  9 / 1134 MEDLINE  
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[PMID]:28321006
[Au] Autor:Habibi S; Ayubi AG; Ohkama-Ohtsu N; Sekimoto H; Yokoyama T
[Ad] Endereço:United Graduate School of Agriculture, Tokyo University of Agriculture and Technology.
[Ti] Título:Genetic Characterization of Soybean Rhizobia Isolated from Different Ecological Zones in North-Eastern Afghanistan.
[So] Source:Microbes Environ;32(1):71-79, 2017 Mar 31.
[Is] ISSN:1347-4405
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Seventy rhizobial isolates were obtained from the root nodules of two soybean (Glycine max) cultivars: Japanese cultivar Enrei and USA cultivar Stine3300, which were inoculated with different soil samples from Afghanistan. In order to study the genetic properties of the isolates, the DNA sequences of the 16S rRNA gene and symbiotic genes (nodD1 and nifD) were elucidated. Furthermore, the isolates were inoculated into the roots of two soybean cultivars, and root nodule numbers and nitrogen fixation abilities were subsequently evaluated in order to assess symbiotic performance. Based on 16S rRNA gene sequences, the Afghanistan isolates obtained from soybean root nodules were classified into two genera, Bradyrhizobium and Ensifer. Bradyrhizobium isolates accounted for 54.3% (38) of the isolates, and these isolates had a close relationship with Bradyrhizobium liaoningense and B. yuanmingense. Five out of the 38 Bradyrhizobium isolates showed a novel lineage for B. liaoningense and B. yuanmingense. Thirty-two out of the 70 isolates were identified as Ensifer fredii. An Ensifer isolate had identical nodD1 and nifD sequences to those in B. yuanmingense. This result indicated that the horizontal gene transfer of symbiotic genes occurred from Bradyrhizobium to Ensifer in Afghanistan soil. The symbiotic performance of the 14 tested isolates from the root nodules of the two soybean cultivars indicated that Bradyrhizobium isolates exhibited stronger acetylene reduction activities than Ensifer isolates. This is the first study to genetically characterize soybean-nodulating rhizobia in Afghanistan soil.
[Mh] Termos MeSH primário: Bradyrhizobium/classificação
Bradyrhizobium/isolamento & purificação
Rhizobiaceae/classificação
Rhizobiaceae/isolamento & purificação
Nódulos Radiculares de Plantas/microbiologia
Feijão de Soja/microbiologia
[Mh] Termos MeSH secundário: Afeganistão
Proteínas de Bactérias/genética
Bradyrhizobium/genética
Bradyrhizobium/fisiologia
Análise por Conglomerados
DNA Bacteriano/química
DNA Bacteriano/genética
DNA Ribossômico/química
DNA Ribossômico/genética
Fixação de Nitrogênio
Filogenia
RNA Ribossômico 16S/genética
Rhizobiaceae/genética
Rhizobiaceae/fisiologia
Análise de Sequência de DNA
Simbiose
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (DNA, Bacterial); 0 (DNA, Ribosomal); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170414
[Lr] Data última revisão:
170414
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170322
[St] Status:MEDLINE
[do] DOI:10.1264/jsme2.ME16119


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[PMID]:28260194
[Au] Autor:Pandit R; Patel R; Patel N; Bhatt V; Joshi C; Singh PK; Kunjadia A
[Ad] Endereço:Department of Animal Biotechnology, College of Veterinary Science and A.H., Anand Agricultural University, Anand, 388 001, Gujarat, India.
[Ti] Título:RNA-Seq reveals the molecular mechanism of trapping and killing of root-knot nematodes by nematode-trapping fungi.
[So] Source:World J Microbiol Biotechnol;33(4):65, 2017 Apr.
[Is] ISSN:1573-0972
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Nematode-trapping fungi are well known for their inherent potential to trap and kill nematodes using specialized trapping devices. However, the molecular mechanisms underlying the trapping and subsequent processes are still unclear. Therefore, in this study, we examined differential genes expression in two nematode-trapping fungi after baiting with nematode extracts. In Arthrobotrys conoides, 809 transcripts associated with diverse functions such as signal transduction, morphogenesis, stress response and peroxisomal proteins, proteases, chitinases and genes involved in the host-pathogen interaction showed differential expression with fold change (>±1.5 fold) in the presence of nematode extract with FDR (p-value < 0.001). G-proteins and mitogen activated protein kinases are considered crucial for signal transduction mechanism. Results of qRT-PCR of 20 genes further validated the sequencing data. Further, variations in gene expression among Duddingtonia flagrans and A. conoides showed septicity of nematode-trapping fungi for its host. The findings illustrate the molecular mechanism of fungal parasitism in A. conoides which may be helpful in developing a potential biocontrol agent against parasitic nematodes.
[Mh] Termos MeSH primário: Ascomicetos/fisiologia
Proteínas Fúngicas/genética
Nematoides/microbiologia
Nódulos Radiculares de Plantas/microbiologia
Análise de Sequência de RNA/métodos
[Mh] Termos MeSH secundário: Animais
Ascomicetos/genética
Ascomicetos/isolamento & purificação
Regulação Fúngica da Expressão Gênica
Interações Hospedeiro-Patógeno
Controle de Pragas
RNA Fúngico/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungal Proteins); 0 (RNA, Fungal)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170306
[St] Status:MEDLINE
[do] DOI:10.1007/s11274-017-2232-7



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