Base de dados : MEDLINE
Pesquisa : A19 [Categoria DeCS]
Referências encontradas : 71 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 8 ir para página                    

  1 / 71 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28250232
[Au] Autor:O'Toole ET; Giddings TH; Winey M
[Ad] Endereço:Boulder Laboratory for 3-D Electron Microscopy of Cells, Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309-0347 eileen.otoole@colorado.edu mark.winey@colorado.edu.
[Ti] Título:Building Cell Structures in Three Dimensions: Electron Tomography Methods for Budding Yeast.
[So] Source:Cold Spring Harb Protoc;2017(3):pdb.top077685, 2017 Mar 01.
[Is] ISSN:1559-6095
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:has been an important model system for numerous cellular, genetic, and molecular studies. However, this small eukaryote presents a challenge for imaging at the electron microscope level. Preparation of yeast using high-pressure freezing followed by freeze-substitution (HPF/FS) results in excellent preservation of cell structure in these difficult-to-fix samples. In particular, cells prepared by HPF/FS can be used for 3D electron tomography (ET) studies where optimum cell preservation is critical. Here, we discuss the advantages of using HPF/FS for ET and show examples of the utility of this method for building yeast cell structures in three dimensions.
[Mh] Termos MeSH primário: Tomografia com Microscopia Eletrônica/métodos
Estruturas Fúngicas/ultraestrutura
Imagem Tridimensional/métodos
Saccharomyces cerevisiae/ultraestrutura
[Mh] Termos MeSH secundário: Substituição ao Congelamento
Congelamento
Preservação Biológica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170303
[St] Status:MEDLINE
[do] DOI:10.1101/pdb.top077685


  2 / 71 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28250212
[Au] Autor:O'Toole ET; Giddings TH; Winey M
[Ad] Endereço:Boulder Laboratory for 3-D Electron Microscopy of Cells, Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309-0347 eileen.otoole@colorado.edu mark.winey@colorado.edu.
[Ti] Título:Cryopreparation and Electron Tomography of Yeast Cells.
[So] Source:Cold Spring Harb Protoc;2017(3):pdb.prot085589, 2017 Mar 01.
[Is] ISSN:1559-6095
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Three-dimensional imaging of cells using electron tomography enables analysis of cell structure at unprecedented resolution. The preparation of cells for tomography using rapid freezing followed by freeze-substitution is an essential first step to ensure the optimal preservation of the cell structure for 3D studies. This protocol outlines a method for obtaining well-preserved cells using high-pressure freezing followed by freeze-substitution. We have found that this method is particularly well suited for electron tomography studies and has the added bonus of preserving antigenicity for immuno-electron microscopy. The steps involved in imaging cells and performing tomographic analysis of cellular structures are also outlined.
[Mh] Termos MeSH primário: Tomografia com Microscopia Eletrônica/métodos
Congelamento
Preservação Biológica
Saccharomyces cerevisiae/ultraestrutura
[Mh] Termos MeSH secundário: Substituição ao Congelamento
Estruturas Fúngicas/ultraestrutura
Pressão Hidrostática
Imagem Tridimensional
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170303
[St] Status:MEDLINE
[do] DOI:10.1101/pdb.prot085589


  3 / 71 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27387217
[Au] Autor:Zhang X; Zhu Y; Bao L; Gao L; Yao G; Li Y; Yang Z; Li Z; Zhong Y; Li F; Yin H; Qu Y; Qin Y
[Ad] Endereço:National Glycoengineering Research Center and State Key Lab of Microbial Technology, Shandong University, Jinan 250100, China; Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong University, Jinan 250100, China. Electronic address: 1219464954@qq.com.
[Ti] Título:Putative methyltransferase LaeA and transcription factor CreA are necessary for proper asexual development and controlling secondary metabolic gene cluster expression.
[So] Source:Fungal Genet Biol;94:32-46, 2016 09.
[Is] ISSN:1096-0937
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The morphological development of fungi is a complex process and is often coupled with secondary metabolite production. In this study, we assessed the function of putative methyltransferase LaeA and transcription factor CreA in controlling asexual development and secondary metabolic gene cluster expression in Penicillium oxalicum. The deletion of laeA (ΔlaeA) impaired the conidiation in P. oxalicum, with a downregulated expression of brlA. Overexpression of P. oxalicum brlA in ΔlaeA could upregulate brlA and abaA remarkably, but could not rescue the conidiation defect; therefore, brlA and abaA expression were necessary but not sufficient for conidiation. Deletion of creA in ΔlaeA background (ΔlaeAΔcreA) blocked conidiation with a white fluffy phenotype. Nutrient-rich medium could not rescue developmental defects in ΔlaeAΔcreA mutant but could rescue defects in ΔlaeA. Expression of 10 genes, namely, albA/wA, abrB/yA, arpA, aygA, arpA-like, arpB, arpB-like, rodA, rodA-like, and rodB, for pigmentation and spore wall protein genes was silenced in ΔlaeAΔcreA, whereas only six of them were downregulated in ΔlaeA. Among the 28 secondary metabolism gene clusters in P. oxalicum, four secondary metabolism gene clusters were silenced in ΔlaeA and two were also silenced in ΔbrlA mutant. A total of 10 physically linked and coregulated genes were distributed over five chromosomes in ΔlaeA. Six of these genes were located in subtelomeric regions, thus demonstrating a positional bias for LaeA-regulated clusters toward subtelomeric regions. All of silenced clusters located in subtelomeric regions were derepressed in ΔlaeAΔcreA, hence showing that lack of CreA could remediate the repression of gene clusters in ΔlaeA background. Results show that both putative methyltransferase LaeA and transcription factor CreA are necessary for proper asexual development and controlling secondary metabolic gene cluster expression.
[Mh] Termos MeSH primário: Proteínas Fúngicas/metabolismo
Regulação Fúngica da Expressão Gênica
Genes Fúngicos
Metiltransferases/metabolismo
Família Multigênica
Penicillium/enzimologia
Fatores de Transcrição/metabolismo
[Mh] Termos MeSH secundário: Proteínas Fúngicas/genética
Estruturas Fúngicas
Deleção de Genes
Inativação Gênica
Metiltransferases/genética
Mutação
Penicillium/genética
Penicillium/crescimento & desenvolvimento
Fatores de Transcrição/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Fungal Proteins); 0 (Transcription Factors); EC 2.1.1.- (Methyltransferases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171117
[Lr] Data última revisão:
171117
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160709
[St] Status:MEDLINE


  4 / 71 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26280456
[Au] Autor:Teranishi K
[Ad] Endereço:Graduate School of Bioresources, Mie University, 1577 Kurimamachiya, Tsu, Mie, Japan.
[Ti] Título:Localization of the bioluminescence system in the pileus of Mycena chlorophos.
[So] Source:Luminescence;31(2):594-9, 2016 Mar.
[Is] ISSN:1522-7243
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Mycena chlorophos, which is primarily distributed in Southeast Asia, is a luminous fungus that emits a bright green light from its pileus for about 2 days at approximately 20°C and high relative humidity. The distribution of bioluminescent tissues in the whole pileus and its sections was heterogeneous. The light intensity in the cap and the upper region of the gill was greater than that in the lower region of the gill. At the microscopic level, the light was predominantly emitted from the membranes of hymenium and basidia cells on the gill. The emission was both cell and region specific. The luminescence system was localized in the cell membrane, and a part of the system was on the cell membrane surface.
[Mh] Termos MeSH primário: Basidiomycota/química
Estruturas Fúngicas/química
Medições Luminescentes
[Mh] Termos MeSH secundário: Carpóforos
Luminescência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170111
[Lr] Data última revisão:
170111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150818
[St] Status:MEDLINE
[do] DOI:10.1002/bio.3001


  5 / 71 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:25901357
[Au] Autor:Marroquin-Guzman M; Wilson RA
[Ad] Endereço:Department of Plant Pathology, University of Nebraska-Lincoln, Lincoln, Nebraska, United States of America.
[Ti] Título:GATA-Dependent Glutaminolysis Drives Appressorium Formation in Magnaporthe oryzae by Suppressing TOR Inhibition of cAMP/PKA Signaling.
[So] Source:PLoS Pathog;11(4):e1004851, 2015 Apr.
[Is] ISSN:1553-7374
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Fungal plant pathogens are persistent and global food security threats. To invade their hosts they often form highly specialized infection structures, known as appressoria. The cAMP/ PKA- and MAP kinase-signaling cascades have been functionally delineated as positive-acting pathways required for appressorium development. Negative-acting regulatory pathways that block appressorial development are not known. Here, we present the first detailed evidence that the conserved Target of Rapamycin (TOR) signaling pathway is a powerful inhibitor of appressorium formation by the rice blast fungus Magnaporthe oryzae. We determined TOR signaling was activated in an M. oryzae mutant strain lacking a functional copy of the GATA transcription factor-encoding gene ASD4. Δasd4 mutant strains could not form appressoria and expressed GLN1, a glutamine synthetase-encoding orthologue silenced in wild type. Inappropriate expression of GLN1 increased the intracellular steady-state levels of glutamine in Δasd4 mutant strains during axenic growth when compared to wild type. Deleting GLN1 lowered glutamine levels and promoted appressorium formation by Δasd4 strains. Furthermore, glutamine is an agonist of TOR. Treating Δasd4 mutant strains with the specific TOR kinase inhibitor rapamycin restored appressorium development. Rapamycin was also shown to induce appressorium formation by wild type and Δcpka mutant strains on non-inductive hydrophilic surfaces but had no effect on the MAP kinase mutant Δpmk1. When taken together, we implicate Asd4 in regulating intracellular glutamine levels in order to modulate TOR inhibition of appressorium formation downstream of cPKA. This study thus provides novel insight into the metabolic mechanisms that underpin the highly regulated process of appressorium development.
[Mh] Termos MeSH primário: Proteínas Quinases Dependentes de AMP Cíclico/metabolismo
Proteínas Fúngicas/metabolismo
Fator de Transcrição GATA4/metabolismo
Glutamato-Amônia Ligase/metabolismo
Magnaporthe/fisiologia
Transdução de Sinais
Serina-Treonina Quinases TOR/antagonistas & inibidores
[Mh] Termos MeSH secundário: Antifúngicos/farmacologia
Chaperoninas/genética
Chaperoninas/metabolismo
AMP Cíclico/metabolismo
Proteínas Quinases Dependentes de AMP Cíclico/genética
Ativação Enzimática/efeitos dos fármacos
Proteínas Fúngicas/agonistas
Proteínas Fúngicas/antagonistas & inibidores
Proteínas Fúngicas/genética
Estruturas Fúngicas/efeitos dos fármacos
Estruturas Fúngicas/enzimologia
Estruturas Fúngicas/fisiologia
Fator de Transcrição GATA4/genética
Deleção de Genes
Regulação Fúngica da Expressão Gênica/efeitos dos fármacos
Glutamato-Amônia Ligase/genética
Glutamina/metabolismo
Interações Hospedeiro-Patógeno/efeitos dos fármacos
Magnaporthe/efeitos dos fármacos
Magnaporthe/enzimologia
Mutação
Fixação de Nitrogênio/efeitos dos fármacos
Oryza/microbiologia
Inibidores de Proteínas Quinases/farmacologia
Subunidades Proteicas/genética
Subunidades Proteicas/metabolismo
Transdução de Sinais/efeitos dos fármacos
Sirolimo/farmacologia
Serina-Treonina Quinases TOR/química
Serina-Treonina Quinases TOR/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Fungal Proteins); 0 (GATA4 Transcription Factor); 0 (Protein Kinase Inhibitors); 0 (Protein Subunits); 0RH81L854J (Glutamine); E0399OZS9N (Cyclic AMP); EC 2.7.1.1 (TOR Serine-Threonine Kinases); EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases); EC 3.6.1.- (Chaperonins); EC 6.3.1.2 (Glutamate-Ammonia Ligase); W36ZG6FT64 (Sirolimus)
[Em] Mês de entrada:1603
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150423
[St] Status:MEDLINE
[do] DOI:10.1371/journal.ppat.1004851


  6 / 71 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:25830634
[Au] Autor:Zhang Q; Berkey R; Pan Z; Wang W; Zhang Y; Ma X; King H; Xiao S
[Ad] Endereço:a Institute for Bioscience and Biotechnology Research ; University of Maryland ; Rockville , MD USA.
[Ti] Título:Dominant negative RPW8.2 fusion proteins reveal the importance of haustorium-oriented protein trafficking for resistance against powdery mildew in Arabidopsis.
[So] Source:Plant Signal Behav;10(3):e989766, 2015.
[Is] ISSN:1559-2324
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Powdery mildew fungi form feeding structures called haustoria inside epidermal cells of host plants to extract photosynthates for their epiphytic growth and reproduction. The haustorium is encased by an interfacial membrane termed the extrahaustorial membrane (EHM). The atypical resistance protein RPW8.2 from Arabidopsis is specifically targeted to the EHM where RPW8.2 activates haustorium-targeted (thus broad-spectrum) resistance against powdery mildew fungi. EHM-specific localization of RPW8.2 suggests the existence of an EHM-oriented protein/membrane trafficking pathway during EHM biogenesis. However, the importance of this specific trafficking pathway for host defense has not been evaluated via a genetic approach without affecting other trafficking pathways. Here, we report that expression of EHM-oriented, nonfunctional RPW8.2 chimeric proteins exerts dominant negative effect over functional RPW8.2 and potentially over other EHM-localized defense proteins, thereby compromising both RPW8.2-mediated and basal resistance to powdery mildew. Thus, our results highlight the importance of the EHM-oriented protein/membrane trafficking pathway for host resistance against haustorium-forming pathogens such as powdery mildew fungi.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/metabolismo
Arabidopsis/metabolismo
Ascomicetos
Resistência à Doença
Estruturas Fúngicas/metabolismo
Interações Hospedeiro-Patógeno
Doenças das Plantas/microbiologia
[Mh] Termos MeSH secundário: Folhas de Planta
Plantas Geneticamente Modificadas
Transporte Proteico
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (RPW8.2 protein, Arabidopsis)
[Em] Mês de entrada:1512
[Cu] Atualização por classe:160217
[Lr] Data última revisão:
160217
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150402
[St] Status:MEDLINE
[do] DOI:10.4161/15592324.2014.989766


  7 / 71 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:25746619
[Au] Autor:Hung MC; Tsai CC; Hsu TH; Liang ZC; Lin FY; Chang SL; Ho WJ; Hsieh CW
[Ad] Endereço:Department of Medicinal Botanicals and Health Applications, Da-Yeh University, Datsuen, Changhua, 51591, Taiwan (R. O. C.).
[Ti] Título:Biological Activities of the Polysaccharides Produced from Different Sources of Xylaria nigripes (Ascomycetes), a Chinese Medicinal Fungus.
[So] Source:Int J Med Mushrooms;17(2):141-50, 2015.
[Is] ISSN:1940-4344
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Xylaria nigripes, a local rare medicinal fungus, has multi-antioxidant activities owing to its water extraction as shown by previous research. However, the main indicator causing the antioxidant effect was not clear, so this research focused on the antioxidant activities from different sources of X. nigripes such as fruiting body polysaccharides, mycelium intracellular polysaccharides, mycelium extracellular polysaccharides, and their deproteinization products. The mycelium intracellular polysaccharide (XnIPS-1) from X. nigripes showed the highest reducing power of antioxidant activity, since it revealed the lowest IC50 values in all the assayed methodologies. The IC50 values of chelating ferrous ion ability, ABTS radical scavenging activity, and DPPH free radical scavenging were 1412, 174.25, and 351.56 µg/mL, respectively. In addition to these results, this research also explored the mechanism between polysaccharides and antioxidants compared by FT-IR analysis. The spectrum shows that the X. nigripes polysaccharide structure changed after the proteins were removed.
[Mh] Termos MeSH primário: Antioxidantes/farmacologia
Produtos Biológicos/farmacologia
Polissacarídeos Fúngicos/farmacologia
Micélio/química
Xylariales/química
[Mh] Termos MeSH secundário: Benzotiazóis/metabolismo
Compostos de Bifenilo/metabolismo
Medicamentos de Ervas Chinesas/farmacologia
Estruturas Fúngicas/química
Concentração Inibidora 50
Estrutura Molecular
Picratos/metabolismo
Ácidos Sulfônicos/metabolismo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antioxidants); 0 (Benzothiazoles); 0 (Biological Products); 0 (Biphenyl Compounds); 0 (Drugs, Chinese Herbal); 0 (Fungal Polysaccharides); 0 (Picrates); 0 (Sulfonic Acids); 28752-68-3 (2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid); DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl)
[Em] Mês de entrada:1512
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150310
[St] Status:MEDLINE


  8 / 71 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:25065672
[Au] Autor:Yan F; Xu S; Guo J; Chen Q; Meng Q; Zheng X
[Ad] Endereço:Department of Food Science and Nutrition, Zhejiang University, Hangzhou, 310058, People's Republic of China; Fuli Institute of Food Science, Zhejiang University, Hangzhou, 310058, People's Republic of China; Zhejiang Key laboratory for agro-food processing, Zhejiang University, Hangzhou, 310058, People's Republic of China.
[Ti] Título:Biocontrol of post-harvest Alternaria alternata decay of cherry tomatoes with rhamnolipids and possible mechanisms of action.
[So] Source:J Sci Food Agric;95(7):1469-74, 2015 May.
[Is] ISSN:1097-0010
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Rhamnolipids were reported to have evident antifungal activity. The efficacy of rhamnolipids against Alternaria alternata and their possible mechanisms involved were investigated. RESULT: The decay incidences of A. alternata of cherry tomatoes (Lycopersicon esculentum) treated by rhamnolipids were significantly reduced. The in vitro assays showed that rhamnolipids inhibited fungal growth on solid medium and prevented spore germination and mycelium growth in liquid medium. In addition, the combination of rhamnolipids and essential oil had a synergistic effect leading to the decrease of fungicidal concentrations of laurel oil. Scanning electron microscopy and transmission electron microscopy observations of the pathogen revealed significant morphological and cell structural alterations in the hyphae. Compared to the control, the content of nucleic acid in supernatant of the suspension of A. alternata increased, while the content of DNA and protein of mycelium decreased, which was in agreement with electrolyte leakage experiments. CONCLUSION: Rhamnolipids could be an alternative to chemicals for controlling post-harvest phytopathogenic fungi on fruits and vegetables.
[Mh] Termos MeSH primário: Alternaria/efeitos dos fármacos
Antifúngicos/farmacologia
Conservação de Alimentos/métodos
Frutas/microbiologia
Fungicidas Industriais/farmacologia
Glicolipídeos/farmacologia
Lycopersicon esculentum/microbiologia
[Mh] Termos MeSH secundário: Alternaria/genética
Alternaria/crescimento & desenvolvimento
Microbiologia de Alimentos
Estruturas Fúngicas/efeitos dos fármacos
Estruturas Fúngicas/crescimento & desenvolvimento
Seres Humanos
Óleos Voláteis/farmacologia
Sesquiterpenos/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Fungicides, Industrial); 0 (Glycolipids); 0 (Oils, Volatile); 0 (Sesquiterpenes); 0 (rhamnolipid); 8002-41-3 (laurel oil)
[Em] Mês de entrada:1603
[Cu] Atualização por classe:150401
[Lr] Data última revisão:
150401
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140729
[St] Status:MEDLINE
[do] DOI:10.1002/jsfa.6845


  9 / 71 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:24528637
[Au] Autor:Manohar CS; Boekhout T; Müller WH; Stoeck T
[Ad] Endereço:CSIR-National Institute of Oceanography, Dona Paula, Goa 403 004, India; Ecology Group, University of Kaiserslautern, Kaiserslautern 67663, Germany. Electronic address: cathrine@nio.org.
[Ti] Título:Tritirachium candoliense sp. nov., a novel basidiomycetous fungus isolated from the anoxic zone of the Arabian Sea.
[So] Source:Fungal Biol;118(2):139-49, 2014 Feb.
[Is] ISSN:1878-6146
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A fungal culture (FCAS11) was isolated from coastal sediments of the Arabian Sea during the anoxic season. Multigene phylogenetic analyses confidentially place the organism as a novel species within the recently defined class Tritirachiomycetes, subphylum Pucciniomycotina, phylum Basidiomycota. We named the new species Tritirachium candoliense and provide the first description of a member of this class from a marine environment. DNA sequences and morphological characters distinguish T. candoliense from previously described Tritirachium species. Its growth characteristics, morphology, and ultrastructural features showed that under anoxic conditions the species grows slowly and produces mainly hyphae with only few blastoconidia. Electron microscopy revealed differences when the culture was exposed to anoxic stress. Notable ultrastructural changes occur for example in mitochondrial cristae, irregularly shaped fat globules and the presence of intracellular membrane invaginations. We assume that the growth characteristics and substrate utilization patterns are an adaptation to its source location, the seasonally anoxic environment of the Arabian Sea.
[Mh] Termos MeSH primário: Basidiomycota/classificação
Basidiomycota/isolamento & purificação
Sedimentos Geológicos/microbiologia
Água do Mar/microbiologia
[Mh] Termos MeSH secundário: Anaerobiose
Basidiomycota/crescimento & desenvolvimento
Basidiomycota/ultraestrutura
Análise por Conglomerados
DNA Fúngico/química
DNA Fúngico/genética
DNA Ribossômico/química
DNA Ribossômico/genética
DNA Espaçador Ribossômico/química
DNA Espaçador Ribossômico/genética
Proteínas Fúngicas/genética
Estruturas Fúngicas/ultraestrutura
Microscopia Eletrônica
Dados de Sequência Molecular
Filogenia
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Fungal); 0 (DNA, Ribosomal); 0 (DNA, Ribosomal Spacer); 0 (Fungal Proteins)
[Em] Mês de entrada:1410
[Cu] Atualização por classe:140217
[Lr] Data última revisão:
140217
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140218
[St] Status:MEDLINE


  10 / 71 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:24528636
[Au] Autor:Werth S; Cheenacharoen S; Scheidegger C
[Ad] Endereço:Biodiversity and Conservation Biology, Swiss Federal Research Institute WSL, Zürcherstrasse 111, CH-8903 Birmensdorf, Switzerland; Faculty of Life and Environmental Sciences, University of Iceland, Sturlugata 7, 101 Reykjavik, Iceland. Electronic address: silke.werth@wsl.ch.
[Ti] Título:Propagule size is not a good predictor for regional population subdivision or fine-scale spatial structure in lichenized fungi.
[So] Source:Fungal Biol;118(2):126-38, 2014 Feb.
[Is] ISSN:1878-6146
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Propagule size has important consequences on the genetic structure of wind-dispersed species, as species with small propagules have higher capability of long-distance dispersal. Here, we studied reproductive modes and compared local and regional population structures in three Macaronesian lichenized fungi differing in propagule size. First, we quantified size distribution of propagules in each species. Second, genotype simulations based on microsatellite data were used to infer the reproductive mode. Third, using spatial analysis and population genetic approaches, we quantified the local and regional scale genetic structures of the fungal species. The three species differed in size distributions of propagules. The majority of populations exhibited clonal reproductive mode. Identical reproductive modes occurred often across species in the same sites, implying a possible relationship between reproductive mode and local site conditions. Contrary to expectation, at the local scale, the species exhibited similar patterns of spatial autocorrelation in genotypes. However, in agreement with the expectation based on propagule size, the species with highest frequency of small vegetative propagules (L. pulmonaria) exhibited lowest regional genetic differentiation. Nevertheless, altogether, our results show that propagule size is not a good predictor of population subdivision in lichenized fungi, neither at local nor regional spatial scale.
[Mh] Termos MeSH primário: Estruturas Fúngicas/citologia
Fungos/citologia
Fungos/crescimento & desenvolvimento
Líquens/microbiologia
Esporos Fúngicos/citologia
[Mh] Termos MeSH secundário: Biota
Genética Populacional
Repetições de Microssatélites
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1410
[Cu] Atualização por classe:140217
[Lr] Data última revisão:
140217
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140218
[St] Status:MEDLINE



página 1 de 8 ir para página                    
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde