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[PMID]:29307563
[Au] Autor:Locatelli Dittrich R; Regidor-Cerrillo J; Ortega-Mora LM; Oliveira Koch M; Busch APB; Gonçalves KA; Cruz AA
[Ad] Endereço:Programa de Pós Graduação em Ciências Veterinárias, Setor de ciências Agrárias, Universidade Federal do Paraná, Rua dos Funcionários, 1540, CEP 80035-050, Curitiba, Paraná, Brazil. Electronic address: roslocdi@ufpr.br.
[Ti] Título:Isolation of Neospora caninum from kidney and brain of a bovine foetus and molecular characterization in Brazil.
[So] Source:Exp Parasitol;185:10-16, 2018 Feb.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bovine neosporosis has become a disease of international concern as it is among the main causes of abortion in cattle. Viable N. caninum has been isolated from brains of fetuses and neonatal calves, and there is no report of isolation of tachyzoites from kidney. Also, detailed information about the genetic diversity of N. caninum is scarce. N. caninum tachyzoites were isolated from the kidney and the brain of an aborted 4-month-old bovine foetus. The parasite was confirmed to be N. caninum by PCR. The tachyzoites of the new isolate, named BNC-PR4, were propagated in Vero cell cultures. Pathogenicity of the parasite was examined in BALB/c mice. Mice inoculated intraperitoneally with BNC-PR4 failed to yield clinical signs of disease and did not induce severe brain lesions, suggesting a bovine isolate with low virulence. The N. caninum-positive DNA sample was further analyzed by multilocus microsatellite (MS) genotyping for MS4, MS5, MS6A, MS6B, MS7, MS8, MS10, MS12, and MS21. Multilocus-microsatellite genotyping revealed a unique genetic profile that differed from previously reported isolates.
[Mh] Termos MeSH primário: Feto Abortado/parasitologia
Encéfalo/parasitologia
Doenças dos Bovinos/parasitologia
Coccidiose/veterinária
Rim/parasitologia
Neospora/isolamento & purificação
[Mh] Termos MeSH secundário: Aborto Animal/parasitologia
Animais
Encéfalo/embriologia
Brasil
Bovinos
Doenças dos Bovinos/embriologia
Cercopithecus aethiops
Coccidiose/embriologia
Coccidiose/parasitologia
DNA de Protozoário/química
DNA de Protozoário/isolamento & purificação
Indústria de Laticínios
Feminino
Rim/embriologia
Camundongos
Camundongos Endogâmicos BALB C
Neospora/genética
Neospora/patogenicidade
Células Vero
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Protozoan)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180109
[St] Status:MEDLINE


  2 / 1611 MEDLINE  
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[PMID]:29210523
[Au] Autor:Harmse R; Wong HN; Smit FJ; Müller J; Hemphill A; N'Da DD; Haynes RK
[Ad] Endereço:Centre of Excellence for Pharmaceutical Sciences, Faculty of Health Sciences, North-West University, Potchefstroom, 2520, South Africa.
[Ti] Título:Activities of 11-Azaartemisinin and N-Sulfonyl Derivatives against Neospora caninum and Comparative Cytotoxicities.
[So] Source:ChemMedChem;12(24):2094-2098, 2017 Dec 19.
[Is] ISSN:1860-7187
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Neosporosis caused by the apicomplexan parasite Neospora caninum is an economically important disease that induces abortion in dairy and beef cattle. There are no vaccines or drugs available on the market for control or treatment of the disease in bovines. The peroxide artemisinin and its derivatives used clinically for treatment of malaria are active against N. caninum and other apicomplexan parasites. We have now evaluated the activities of the readily accessible and chemically robust 11-azaartemisinin 5 and selected N-sulfonyl derivatives prepared as described in the accompanying paper against N. caninum tachyzoites grown in infected human foreskin fibroblasts. Azaartemisinin elicited an IC value of 150 nm, and the 2',5'-dichloro-3'-thienylsulfonyl-11-azaartemisinin 17 was found to be the most active, with an IC value of 40 nm. Comparison with normal human fetal lung fibroblasts HFLF WI-38 revealed relatively benign cytotoxicity. The compounds were also screened in vitro against TK-10 (renal), UACC-62 (melanoma) and MCF-7 (breast) cancer cell lines; overall, in line with activities against HFLF cells, most compounds in the series were found to be inactive.
[Mh] Termos MeSH primário: Antineoplásicos/farmacologia
Antiprotozoários/farmacologia
Artemisininas/farmacologia
Neospora/efeitos dos fármacos
Sulfonas/farmacologia
[Mh] Termos MeSH secundário: Antineoplásicos/síntese química
Antineoplásicos/química
Antiprotozoários/síntese química
Antiprotozoários/química
Artemisininas/síntese química
Artemisininas/química
Linhagem Celular Tumoral
Proliferação Celular/efeitos dos fármacos
Relação Dose-Resposta a Droga
Ensaios de Seleção de Medicamentos Antitumorais
Fibroblastos/efeitos dos fármacos
Prepúcio do Pênis/citologia
Seres Humanos
Masculino
Conformação Molecular
Testes de Sensibilidade Parasitária
Relação Estrutura-Atividade
Sulfonas/síntese química
Sulfonas/química
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Antiprotozoal Agents); 0 (Artemisinins); 0 (Sulfones)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171225
[Lr] Data última revisão:
171225
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171207
[St] Status:MEDLINE
[do] DOI:10.1002/cmdc.201700600


  3 / 1611 MEDLINE  
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[PMID]:28850580
[Au] Autor:Moreno-Torres KI; Pomeroy LW; Moritz M; Saville W; Wolfe B; Garabed R
[Ad] Endereço:The Ohio State University, Department of Veterinary Preventive Medicine, Columbus, Ohio, United States of America.
[Ti] Título:Host species heterogeneity in the epidemiology of Nesopora caninum.
[So] Source:PLoS One;12(8):e0183900, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Pathogen transmission across species drives disease emergence; however, mechanisms by which multi-host pathogens cross species boundaries are not well identified. This knowledge gap prevents integrated and targeted control in an epidemiologically continuous ecosystem. Our goal is to describe the impact of host species heterogeneity on the epidemiology of Neospora caninum circulating between livestock and wildlife in southeastern Ohio. We collected biological samples from Père David's deer (Elaphurus davidianus) located at an outdoor wildlife conservation center; from cattle raised at farms adjacent to the center; and from wild white-tailed deer that roamed across farm and center boundaries. We designed nested infectious disease models of competing hypotheses about transmission and used collected data to fit the models, thereby estimating important immunological and transmission quantities which describe the species-specific contribution to the persistence of this pathogen in the community. We applied these data and models to suggest appropriate species-specific disease control methods. Results show that immunity in cattle and Pére David's deer wanes over time, while in white-tailed deer immunity appears to be lifelong. Transmission quantities for cattle were estimated at values below the threshold for an outbreak (Rt < 1), meaning that chains of transmission are not maintained within this population and infections must occur due to reintroduction from an outside source. Pére David's deer and white-tailed deer both could maintain continuous chains of transmission within their group (Rt > 1). Therefore, we propose that control of contact with outside sources will be useful for disease control in cattle; boosting immunity with vaccines might be an avenue to prevent infection in cattle and Père David's deer. White-tailed deer are a potential maintenance host for infection and require further study to determine optimal control methods. Community-level investigations like this allow us to better evaluate heterogeneities in transmission processes that ultimately guide targeted control.
[Mh] Termos MeSH primário: Coccidiose/veterinária
Especificidade de Hospedeiro
[Mh] Termos MeSH secundário: Animais
Animais Selvagens
Bovinos
Coccidiose/epidemiologia
Coccidiose/transmissão
Cervos
Surtos de Doenças
Neospora
Ohio/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170830
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183900


  4 / 1611 MEDLINE  
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[PMID]:28601062
[Au] Autor:Razmi G; Naseri Z
[Ad] Endereço:Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Azadi Square, P.O. Box 91775-1793, Mashhad, Iran
[Ti] Título:Molecular detection of Neospora caninum infection in ovine aborted foetuses in the Mashhad area, Iran
[So] Source:Ann Parasitol;63(1):45-47, 2017.
[Is] ISSN:2299-0631
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:N. caninum could cause abortion in small ruminants. The aim of the study was to detect N. caninum infection in ovine aborted foetuses in the Mashhad area by PCR examination. During the period 2009 to 2013, 71 ovine aborted foetuses were collected and their brain samples examined by PCR. Of the 71 brains of the aborted foetuses, N. caninum DNA was detected in seven (9.8%) samples. In conclusion, it seems that N. caninum may act as a causative agent of abortion in sheep in the Mashhad area.
[Mh] Termos MeSH primário: Feto Abortado/parasitologia
Aborto Animal/parasitologia
Coccidiose/veterinária
Neospora
Complicações Parasitárias na Gravidez/veterinária
Doenças dos Ovinos/parasitologia
[Mh] Termos MeSH secundário: Animais
Coccidiose/epidemiologia
Coccidiose/parasitologia
Feminino
Irã (Geográfico)/epidemiologia
Gravidez
Complicações Parasitárias na Gravidez/epidemiologia
Complicações Parasitárias na Gravidez/parasitologia
Ovinos
Doenças dos Ovinos/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170706
[Lr] Data última revisão:
170706
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170612
[St] Status:MEDLINE
[do] DOI:10.17420/ap6301.84


  5 / 1611 MEDLINE  
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[PMID]:28527905
[Au] Autor:He X; Gong P; Wei Z; Liu W; Wang W; Li J; Yang Z; Zhang X
[Ad] Endereço:College of Veterinary Medicine, Jilin University, Changchun 130062, China.
[Ti] Título:Peroxisome proliferator-activated receptor-γ-mediated polarization of macrophages in Neospora caninum infection.
[So] Source:Exp Parasitol;178:37-44, 2017 Jul.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Neospora caninum is an apicomplexan parasite closely related Toxoplasma gondii, which causes neurological disease and abortion in multiple animal species. Macrophage polarization plays an important role in host immune responses to parasites infection, such as Toxoplasma gondii, Leishmania, Trypanosoma cruzi. However, the dynamics of macrophage polarization, as well as the possible mechanism that regulate macrophage polarization, during N. caninum infection remains unclear. METHODS: The M1 and M2-phenotypic markers of peritoneal macrophages from mice infected with tachyzoites of Nc-1 were analyzed by flow cytometry (FCM) analysis. Then J774A.1 cells were respectively treated with GW9662 and RGZ, and stimulated by tachyzoites of Nc-1. M1 and M2-phenotypic markers were determined by FCM and ELISA. And the activations of PPAR-γ and NF-κB were determined by Western blotting. RESULTS: In this study, our data showed that macrophages were preferentially differentiated into the M1 type during the acute stage of N. caninum infection, while the level of M2 macrophages significantly increased during the chronic stage of infection. In vitro study, compared with the GW9662 group and RGZ group, N. caninum can promote M2-polarized phenotype through up-regulate the activity of PPAR-γ and inhibting NF-κB activation. CONCLUSION: In conclusion, this study demonstrated that macrophages are plastic since M1 differentiated macrophages can express M2 markers with N. caninum infection through up-regulating the activity of PPAR-γ and inhibting NF-κB activation and may be providing new insights for the prevention and treatment of N. caninum infection.
[Mh] Termos MeSH primário: Coccidiose/parasitologia
Macrófagos Peritoneais/parasitologia
Neospora/fisiologia
PPAR gama/fisiologia
[Mh] Termos MeSH secundário: Animais
Arginase/metabolismo
Antígeno B7-1/metabolismo
Linhagem Celular
Cercopithecus aethiops
Coccidiose/metabolismo
Coccidiose/patologia
Citocinas/metabolismo
Citometria de Fluxo
Interleucina-10/metabolismo
Lectinas Tipo C/metabolismo
Ativação de Macrófagos
Macrófagos Peritoneais/imunologia
Lectinas de Ligação a Manose/metabolismo
Camundongos
Camundongos Endogâmicos BALB C
NF-kappa B/metabolismo
Receptores de Superfície Celular/metabolismo
Transdução de Sinais
Células Vero
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (B7-1 Antigen); 0 (Cytokines); 0 (Lectins, C-Type); 0 (Mannose-Binding Lectins); 0 (NF-kappa B); 0 (PPAR gamma); 0 (Receptors, Cell Surface); 0 (mannose receptor); 130068-27-8 (Interleukin-10); EC 3.5.3.1 (Arg1 protein, mouse); EC 3.5.3.1 (Arginase)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170522
[St] Status:MEDLINE


  6 / 1611 MEDLINE  
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[PMID]:28509641
[Au] Autor:James KE; Smith WA; Conrad PA; Packham AE; Guerrero L; Ng M; Pusterla N
[Ti] Título:Seroprevalences of anti-Sarcocystis neurona and anti-Neospora hughesi antibodies among healthy equids in the United States.
[So] Source:J Am Vet Med Assoc;250(11):1291-1301, 2017 Jun 01.
[Is] ISSN:1943-569X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE To describe the general seroprevalence of anti-Sarcocystis neurona and anti-Neospora hughesi antibodies among healthy equids by use of indirect fluorescent antibody tests and determine potential risk factors for seropositivity. DESIGN Cross-sectional study. SAMPLE Whole blood samples collected from 5,250 equids (1 sample/animal) across 18 states in the United States during October 2013. PROCEDURES Information regarding potential risk factors (geographic region, breed, primary use, sex, and age) was collected along with the blood samples. For each equid, an indirect fluorescent antibody test was used to determine serum titers of antibody against each of the 2 protozoal parasites. Mixed-effects logistic regression models were created to determine ORs for seropositivity. RESULTS The overall seroprevalence of anti-S neurona and anti-N hughesi antibodies in the tested equids was 78% and 34%, respectively. Of the equids, 31% were seropositive and 18% were seronegative for antibodies against both parasites. Factors associated with equids being seropositive for anti-S neurona antibodies were residence in the South, warmblood breed, and age > 5 years. Seroprevalence of anti-N hughesi antibodies did not differ among equids in different states across the country, but warmblood breed and age > 5 years were associated with seropositivity. CONCLUSIONS AND CLINICAL RELEVANCE With regard to risk factors for S neurona and N hughesi exposure and antibody response among tested equids, older age was not unexpected; however, the influences of warmblood breed and geographic location on seropositivity for anti-S neurona antibody but not for anti-N hughesi antibody deserve further investigation.
[Mh] Termos MeSH primário: Coccidiose/veterinária
Encefalomielite/veterinária
Doenças dos Cavalos/epidemiologia
Neospora/isolamento & purificação
Sarcocystis/isolamento & purificação
Sarcocistose/veterinária
[Mh] Termos MeSH secundário: Animais
Anticorpos Antiprotozoários/sangue
Coccidiose/epidemiologia
Coccidiose/parasitologia
Estudos Transversais
Encefalomielite/epidemiologia
Encefalomielite/parasitologia
Feminino
Doenças dos Cavalos/sangue
Doenças dos Cavalos/parasitologia
Cavalos
Masculino
Neospora/imunologia
Prevalência
Fatores de Risco
Sarcocystis/imunologia
Sarcocistose/epidemiologia
Sarcocistose/parasitologia
Estados Unidos/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Protozoan)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170517
[St] Status:MEDLINE
[do] DOI:10.2460/javma.250.11.1291


  7 / 1611 MEDLINE  
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[PMID]:28497227
[Au] Autor:Bártová E; Kobédová K; Lamka J; Kotrba R; Vodicka R; Sedlák K
[Ad] Endereço:Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackého tr. 1946/1, 612 42, Brno, Czech Republic. bartovae@vfu.cz.
[Ti] Título:Seroprevalence of Neospora caninum and Toxoplasma gondii in exotic ruminants and camelids in the Czech Republic.
[So] Source:Parasitol Res;116(7):1925-1929, 2017 Jul.
[Is] ISSN:1432-1955
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Neospora caninum and Toxoplasma gondii are the protozoan parasites with definitive hosts from order Carnivora. Due to vertical transmission, both parasites can cause abortions and neonatal mortality that lead to significant productive and economic losses in the domestic ruminants. The aim of this study was to describe N. caninum and T. gondii seroprevalence in the group of frequently farmed captive exotic ruminants (n = 184) including Bovidae (barbary sheep, bezoar goat, common eland, American bison, water buffalo, and yak) and Camelidae (bactrian camel, guanaco, llama, and alpaca). Antibodies were tested by indirect fluorescent antibody test (IFAT) and enzyme-linked immunosorbent assay (ELISA). Higher prevalence of T. gondii antibodies (31% in IFAT and 52% in ELISA) was detected compared to N. caninum (24% in IFAT and 17% in cELISA). Mixed infection was found in 18 (10%) and 22 (12%) animals by IFAT and ELISA, respectively. Higher seroprevalence of both N. caninum and T. gondii was found in Camelidae compared to Bovidae. To author knowledge, this is the first detection of T. gondii and N. caninum antibodies in common elands and bezoar goats.
[Mh] Termos MeSH primário: Camelidae/parasitologia
Coccidiose/veterinária
Neospora/imunologia
Ruminantes/parasitologia
Toxoplasma/imunologia
Toxoplasmose Animal/epidemiologia
[Mh] Termos MeSH secundário: Animais
Coccidiose/epidemiologia
Coccidiose/parasitologia
República Tcheca/epidemiologia
Ensaio de Imunoadsorção Enzimática/veterinária
Feminino
Técnica Indireta de Fluorescência para Anticorpo/veterinária
Masculino
Prevalência
Estudos Soroepidemiológicos
Toxoplasmose Animal/parasitologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170513
[St] Status:MEDLINE
[do] DOI:10.1007/s00436-017-5470-6


  8 / 1611 MEDLINE  
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[PMID]:28495194
[Au] Autor:Peters M; Osmann C; Wohlsein P; Schares G
[Ad] Endereço:Chemisches und Veterinäruntersuchungsamt Westfalen (CVUA) Westfalen, Zur Taubeneiche 10-12, D-59821 Arnsberg, Germany. Electronic address: martin.peters@cvua-westfalen.de.
[Ti] Título:Neospora caninum abortion in a Malayan tapir (Tapirus indicus).
[So] Source:Vet Parasitol;239:37-41, 2017 May 30.
[Is] ISSN:1873-2550
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A captive 17-year old female Malayan tapir (Tapirus indicus) aborted a fetus with a crown rump length of 19cm in early pregnancy. The fetus showed an early state of mummification. Histologically, a multifocal mononuclear encephalitis, myocarditis and periportal hepatitis was present indicating a possible protozoal cause of abortion. Although immunohistologically, Neospora (N.) caninum antigen could not be demonstrated, N. caninum DNA was detected by Polymerase Chain Reaction (PCR) in brain, heart, liver and lung of the fetus. N. caninum DNA was extracted from the aborted fetus and the microsatellite marker MS10 was amplified by PCR and sequenced. The obtained MS10 microsatellite pattern has not been described in Germany yet. Nevertheless, the MS10 pattern was very similar to those reported for N. caninum isolated from dogs and cattle in Germany. Because of the histological pattern and extent of the lesions, neosporosis was suspected as the cause of fetal death and abortion. This case report describes for the first time transplacental transmission of N. caninum and abortion due to neosporosis in a tapir.
[Mh] Termos MeSH primário: Aborto Animal/parasitologia
Coccidiose/veterinária
Neospora
Perissodáctilos
[Mh] Termos MeSH secundário: Feto Abortado
Animais
Animais de Zoológico
Coccidiose/parasitologia
Coccidiose/patologia
Feminino
Gravidez
Complicações Parasitárias na Gravidez/veterinária
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170513
[St] Status:MEDLINE


  9 / 1611 MEDLINE  
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[PMID]:28495191
[Au] Autor:Sinnott FA; Monte LG; Collares TF; Silveira RM; Borsuk S
[Ad] Endereço:Laboratório de Imunodiagnóstico, Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, Rio Grande do Sul, Brazil.
[Ti] Título:Review on the immunological and molecular diagnosis of neosporosis (years 2011-2016).
[So] Source:Vet Parasitol;239:19-25, 2017 May 30.
[Is] ISSN:1873-2550
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Neosporosis, caused by the apicomplexan protozoan Neospora caninum, is a disease which affects a wide range of mammalian hosts (mainly cattle and dogs). N. caninum infection is considered the major cause of livestock abortions worldwide, and therefore is responsible for great losses in the industry. Because there are no effective treatments or vaccines, diagnosis is essential for pathogen control. Studies of N. caninum mechanisms of pathogenesis have led to the identification of new antigens, including NcSRS2, NcSAG1, Ncp40, NcSUB1, NcMIC10, and NcGRAs; and a variety of molecular and immunological assays, based on these molecules, have been proposed to detect N. caninum in tissues or serum samples. We report advances achieved in the last five years in neosporosis control, based on the immunological and molecular diagnostic tests.
[Mh] Termos MeSH primário: Coccidiose/veterinária
Neospora/imunologia
[Mh] Termos MeSH secundário: Animais
Antígenos de Protozoários/sangue
Antígenos de Protozoários/imunologia
Coccidiose/diagnóstico
Feminino
Imunoensaio/veterinária
Gravidez
Testes Sorológicos/veterinária
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Antigens, Protozoan)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170513
[St] Status:MEDLINE


  10 / 1611 MEDLINE  
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[PMID]:28490374
[Au] Autor:Gutiérrez-Expósito D; García-Bocanegra I; Howe DK; Arenas-Montes A; Yeargan MR; Ness SL; Ortega-Mora LM; Álvarez-García G
[Ad] Endereço:SALUVET, Animal Health Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Ciudad Universitaria s/n, 28040, Madrid, Spain.
[Ti] Título:A serosurvey of selected cystogenic coccidia in Spanish equids: first detection of anti-Besnoitia spp. specific antibodies in Europe.
[So] Source:BMC Vet Res;13(1):128, 2017 May 10.
[Is] ISSN:1746-6148
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Equine besnoitiosis, caused by Besnoitia bennetti, and equine protozoal myeloencephalitis (EPM), caused by Sarcocystis neurona and Neospora hughesi are relevant equine diseases in the Americas that have been scarcely studied in Europe. Thus, a serosurvey of these cystogenic coccidia was carried out in Southern Spain. A cross-sectional study was performed and serum samples from horses (n = 553), donkeys (n = 85) and mules (n = 83) were included. An in-house enzyme-linked immunosorbent assay (ELISA) was employed to identify a Besnoitia spp. infection and positive results were confirmed by an a posteriori western blot. For Neospora spp. and Sarcocystis spp., infections were detected using in-house ELISAs based on the parasite surface antigens N. hughesi rNhSAG1 and S. neurona rSnSAG2/3/4. Risk factors associated with these protozoan infections were also investigated. RESULTS: Antibodies against Besnoitia spp., Neospora spp. and Sarcocystis spp. infections were detected in 51 (7.1%), 46 (6.4%) and 20 (2.8%) of 721 equids, respectively. The principal risk factors associated with a higher seroprevalence of Besnoitia spp. were the host species (mule or donkey), the absence of shelter and the absence of a rodent control programme. The presence of rodents was the only risk factor for Neospora spp. infection. CONCLUSIONS: This study was the first extensive serosurvey of Besnoitia spp. infection in European equids accomplished by two complementary tests and gives evidence of the presence of specific antibodies in these populations. However, the origin of the infection is still unclear. Further parasite detection and molecular genotyping are needed to identify the causative Besnoitia and Neospora species. Finally, cross-reactions with antibodies directed against other species of Sarcocystis might explain the positive reactions against the S. neurona antigens.
[Mh] Termos MeSH primário: Anticorpos Antiprotozoários/sangue
Coccídios
Coccidiose/veterinária
Doenças dos Cavalos/parasitologia
Sarcocystidae
[Mh] Termos MeSH secundário: Animais
Coccídios/imunologia
Coccídios/isolamento & purificação
Coccidiose/sangue
Coccidiose/imunologia
Estudos Transversais
Feminino
Doenças dos Cavalos/sangue
Doenças dos Cavalos/imunologia
Cavalos
Masculino
Neospora
Sarcocystidae/imunologia
Sarcocystidae/isolamento & purificação
Sarcocystis
Estudos Soroepidemiológicos
Espanha
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Protozoan)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170512
[St] Status:MEDLINE
[do] DOI:10.1186/s12917-017-1046-z



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