Base de dados : MEDLINE
Pesquisa : B01.043.075.600.580.070.100 [Categoria DeCS]
Referências encontradas : 481 [refinar]
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[PMID]:28985216
[Au] Autor:Hussein HE; Bastos RG; Schneider DA; Johnson WC; Adham FK; Davis WC; Laughery JM; Herndon DR; Alzan HF; Ueti MW; Suarez CE
[Ad] Endereço:Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, United States of America.
[Ti] Título:The Babesia bovis hap2 gene is not required for blood stage replication, but expressed upon in vitro sexual stage induction.
[So] Source:PLoS Negl Trop Dis;11(10):e0005965, 2017 Oct.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Babesia bovis, is a tick borne apicomplexan parasite responsible for important cattle losses globally. Babesia parasites have a complex life cycle including asexual replication in the mammalian host and sexual reproduction in the tick vector. Novel control strategies aimed at limiting transmission of the parasite are needed, but transmission blocking vaccine candidates remain undefined. Expression of HAP2 has been recognized as critical for the fertilization of parasites in the Babesia-related Plasmodium, and is a leading candidate for a transmission blocking vaccine against malaria. Hereby we identified the B. bovis hap2 gene and demonstrated that it is widely conserved and differentially transcribed during development within the tick midgut, but not by blood stage parasites. The hap2 gene was disrupted by transfecting B. bovis with a plasmid containing the flanking regions of the hap2 gene and the GPF-BSD gene under the control of the ef-1α-B promoter. Comparison of in vitro growth between a hap2-KO B. bovis clonal line and its parental wild type strain showed that HAP2 is not required for the development of B. bovis in erythrocytes. However, xanthurenic acid-in vitro induction experiments of sexual stages of parasites recovered after tick transmission resulted in surface expression of HAP2 exclusively in sexual stage induced parasites. In addition, hap2-KO parasites were not able to develop such sexual stages as defined both by morphology and by expression of the B. bovis sexual marker genes 6-Cys A and B. Together, the data strongly suggests that tick midgut stage differential expression of hap2 is associated with the development of B. bovis sexual forms. Overall these studies are consistent with a role of HAP2 in tick stages of the parasite and suggest that HAP2 is a potential candidate for a transmission blocking vaccine against bovine babesiosis.
[Mh] Termos MeSH primário: Vetores Aracnídeos/parasitologia
Babesia bovis/genética
Babesia bovis/fisiologia
Genes de Protozoários
Proteínas de Protozoários/genética
Proteínas de Protozoários/metabolismo
Rhipicephalus/parasitologia
[Mh] Termos MeSH secundário: Animais
Babesia bovis/efeitos dos fármacos
Babesia bovis/crescimento & desenvolvimento
Bovinos/parasitologia
Eritrócitos/parasitologia
Feminino
Estágios do Ciclo de Vida
Fator 1 de Elongação de Peptídeos/genética
Regiões Promotoras Genéticas
Reprodução/efeitos dos fármacos
Reprodução/genética
Xanturenatos/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Peptide Elongation Factor 1); 0 (Protozoan Proteins); 0 (Xanthurenates); 58LAB1BG8J (xanthurenic acid)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171007
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005965


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[PMID]:28655583
[Au] Autor:Rizk MA; El-Sayed SAE; AbouLaila M; Yokoyama N; Igarashi I
[Ad] Endereço:National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-Cho, Obihiro, Hokkaido, Japan; Department of Internal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt.
[Ti] Título:Evaluation of the inhibitory effect of N-acetyl-L-cysteine on Babesia and Theileria parasites.
[So] Source:Exp Parasitol;179:43-48, 2017 Aug.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:N-acetyl-L-cysteine is known to have antibacterial, antiviral, antimalarial, and antioxidant activities. Therefore, the in vitro inhibitory effect of this hit was evaluated in the present study on the growth of Babesia and Theileria parasites. The in vitro growth of Babesia bovis, Babesia bigemina, Babesia divergens, Theileria equi, and Babesia caballi that were tested was significantly inhibited (P < 0.05) by micromolar concentrations of N-acetyl-L-cysteine. The inhibitory effect of N-acetyl-L-cysteine was synergistically potentiated when used in combination with diminazene aceturate on B. bovis and B. caballi cultures. These results indicate that N-acetyl-L-cysteine might be used as a drug for the treatment of babesiosis, especially when used in combination with diminazene aceturate.
[Mh] Termos MeSH primário: Acetilcisteína/farmacologia
Antiprotozoários/farmacologia
Babesia/efeitos dos fármacos
Diminazena/análogos & derivados
Theileria/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Babesia/crescimento & desenvolvimento
Babesia bovis/efeitos dos fármacos
Babesia bovis/crescimento & desenvolvimento
Bovinos
Diminazena/farmacologia
Sinergismo Farmacológico
Eritrócitos/parasitologia
Cavalos
Concentração Inibidora 50
Espectrometria de Fluorescência
Theileria/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antiprotozoal Agents); JI8SAD85NO (diminazene aceturate); WYQ7N0BPYC (Acetylcysteine); Y5G36EEA5Z (Diminazene)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170726
[Lr] Data última revisão:
170726
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170629
[St] Status:MEDLINE


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[PMID]:28153804
[Au] Autor:Rojas-Martínez C; Rodríguez-Vivas RI; Figueroa Millán JV; Acosta Viana KY; Gutiérrez Ruiz EJ; Álvarez Martínez JA
[Ad] Endereço:CENID-Parasitología Veterinaria INIFAP, Carr. Fed. Cuernavaca-Cuautla No. 8534, Col. Progreso, C.P. 62550, Jiutepec, Morelos, Mexico; Campus de Ciencias Biológicas y Agropecuarias, FMVZ, Universidad Autónoma de Yucatán, km. 15.5 Carretera Mérida-Xmatkuil, Mérida, Yucatán, Mexico.
[Ti] Título:Putrescine: Essential factor for in vitro proliferation of Babesia bovis.
[So] Source:Exp Parasitol;175:79-84, 2017 Apr.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This study reports the effect of putrescine addition, either alone or in combination with insulin, transferrin and selenite (ITS), to serum-free Advanced DMEM/F12 (A-DMEM/F12) medium, on the in vitro culture of Babesia bovis and using a perfusion bioreactor to improve efficiency of the process. A B. bovis strain previously adapted to proliferate in serum-free medium (Bbovis-SF) was evaluated using eight increasing concentrations of putrescine. The percentage of parasitized erythrocytes (PPE) obtained from cultures supplemented with 0.101 mg/L was 6.23% compared with 2.3% for control cultures with M199 with Earle's salts (M199) and 40% serum. The combination of putrescine (0.101 mg/L) and a mixture of ITS (2000, 1100, and 1.34 mg/L, respectively) (Pu-ITS), in A-DMEM/F12 culture medium without serum yielded a maximum PPE of 17.26% compared to 2.58% in the control medium. This new formulation of culture medium, together with the use of a hollow-fiber perfusion bioreactor system (HFPBS), caused a substantial increase in the proliferation of B. bovis, yielding a maximum cumulative PPE of 118.8% after five days, compared to 58.6% in cultures treated with control medium M199 and 40% serum. We concluded that the addition of the ITS mixture and putrescine to the culture medium stimulated the proliferation of B. bovis in vitro. This new medium formulation, used in a HFPBS culture system, can be an effective, automated-prone system that can induce massive proliferation of B. bovis for use as a source of parasite antigens and immunogens.
[Mh] Termos MeSH primário: Babesia bovis/crescimento & desenvolvimento
Reatores Biológicos
Eritrócitos/parasitologia
Putrescina/metabolismo
[Mh] Termos MeSH secundário: Animais
Reatores Biológicos/parasitologia
Reatores Biológicos/veterinária
Bovinos
Criopreservação/veterinária
Meios de Cultura Livres de Soro
Insulina/metabolismo
Ácido Selenioso/metabolismo
Transferrina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Culture Media, Serum-Free); 0 (Insulin); 0 (Transferrin); F6A27P4Q4R (Selenious Acid); V10TVZ52E4 (Putrescine)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170328
[Lr] Data última revisão:
170328
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170204
[St] Status:MEDLINE


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[PMID]:28152313
[Au] Autor:Johnson WC; Taus NS; Reif KE; Bohaliga GA; Kappmeyer LS; Ueti MW
[Ad] Endereço:Animal Disease Research Unit, USDA-ARS , Pullman, Washington 99164, United States.
[Ti] Título:Analysis of Stage-Specific Protein Expression during Babesia Bovis Development within Female Rhipicephalus Microplus.
[So] Source:J Proteome Res;16(3):1327-1338, 2017 Mar 03.
[Is] ISSN:1535-3907
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Arthropod-borne protozoan pathogens have a complex life cycle that includes asexual reproduction of haploid stages in mammalian hosts and the development of diploid stages in invertebrate hosts. The ability of pathogens to invade, survive, and replicate within distinct cell types is required to maintain their life cycle. In this study, we describe a comparative proteomic analysis of a cattle pathogen, Babesia bovis, during its development within the mammalian and tick hosts with the goal of identifying cell-surface proteins expressed by B. bovis kinetes as potential targets for the development of a transmission blocking vaccine. To determine parasite tick-stage-specific cell-surface proteins, CyDye labeling was performed with B. bovis blood stages from the bovine host and kinetes from the tick vector. Cell-surface kinete-stage-specific proteins were identified using 2D difference in gel electrophoresis and analyzed by mass spectrometry. Ten proteins were identified as kinete-stage-specific, with orthologs found in closely related Apicomplexan pathogens. Transcriptional analysis revealed two genes were highly expressed by kinetes as compared with blood stages. Immunofluorescence using antibodies against the two proteins confirmed kinete-stage-specific expression. The identified cell-surface kinete proteins are potential candidates for the development of a B. bovis transmission blocking vaccine.
[Mh] Termos MeSH primário: Babesia bovis/química
Estágios do Ciclo de Vida/fisiologia
Proteômica/métodos
Rhipicephalus/microbiologia
[Mh] Termos MeSH secundário: Animais
Babesia bovis/crescimento & desenvolvimento
Bovinos
Feminino
Perfilação da Expressão Gênica
Espectrometria de Massas
Proteínas de Membrana/análise
Proteínas de Membrana/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Membrane Proteins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170203
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jproteome.6b00947


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Machado, Rosangela Zacarias
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[PMID]:28089650
[Au] Autor:Giglioti R; Oliveira HN; Ibelli AM; Bilhassi TB; Néo TA; Santana CH; Rabelo MD; Machado RZ; de Souza Chagas AC; de Sena Oliveira MC
[Ad] Endereço:Universidade Estadual Júlio de Mesquita Filho, Jaboticabal, SP, Brazil. Electronic address: gigliotirodrigo@gmail.com.
[Ti] Título:Neither quantification by qPCR nor quantitative Elisa can be used to discriminate Angus cattle for resistance/susceptibility to Babesia bovis.
[So] Source:Ticks Tick Borne Dis;8(3):335-340, 2017 Mar.
[Is] ISSN:1877-9603
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:With the aim of finding quantitative phenotypic traits that can be used to discriminate the levels of resistance/susceptibility to Babesia bovis, we estimated the repeatability and correlation between the level of infection, determined by the number of copies of a fragment of the gene that encodes cytochrome B (NC mt-cyB) of B. bovis, and the levels of the anti-B. bovis antibodies, in blood samples collected from 51 Angus cattle on two different occasions. Samples with the anticoagulant EDTA were used for DNA extraction and without anticoagulant for separation of the blood serum. The quantification of the NC mt-cyB of B. bovis was carried out by the quantitative PCR technique (qPCR), while the anti-B. bovis IgG antibody titers (S/P) were quantified by the ELISA method. The NC and S/P data were log10-transformed to improve the approximation to the normal distribution and were analyzed using mixed models. The correlations between NC mt-cyB and S/P were estimated, as well as the repeatability values for each trait. The results obtained showed the high sensitivity of the techniques, with 100% of the animals being positive for B. bovis, detected by both the serological and molecular tests. The correlations estimated between NC and S/P were low, 0.10 and 0.12, in the first and second collection, respectively. The repeatability estimated for NC was 0.06, whereas for the S/P it was 0.42. The low correlations between S/P and NC in the two collections demonstrated that the variation in the NC value is independent of the level of antibodies. This results indicated that animals with a higher levels of antibodies against B. bovis in the first collection continued to have a higher levels in the second one. However, the very low values for the repeatability value of NC, and for the correlations between S/P and NC, demonstrates that neither NC or S/P could be used to discriminate animals for resistance/susceptibility to B. bovis.
[Mh] Termos MeSH primário: Anticorpos Antiprotozoários/sangue
Babesia bovis/imunologia
Babesia bovis/isolamento & purificação
Babesiose/imunologia
Doenças dos Bovinos/imunologia
Ensaio de Imunoadsorção Enzimática
Reação em Cadeia da Polimerase em Tempo Real
[Mh] Termos MeSH secundário: Animais
Babesia bovis/genética
Babesiose/parasitologia
Bovinos/parasitologia
Doenças dos Bovinos/parasitologia
Citocromos b/genética
DNA de Protozoário/sangue
Resistência à Doença
Suscetibilidade a Doenças
Técnicas de Diagnóstico Molecular/métodos
Reação em Cadeia da Polimerase em Tempo Real/métodos
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Protozoan); 0 (DNA, Protozoan); 9035-37-4 (Cytochromes b)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170117
[St] Status:MEDLINE


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[PMID]:28028631
[Au] Autor:Jirapattharasate C; Adjou Moumouni PF; Cao S; Iguchi A; Liu M; Wang G; Zhou M; Vudriko P; Efstratiou A; Changbunjong T; Sungpradit S; Ratanakorn P; Moonarmart W; Sedwisai P; Weluwanarak T; Wongsawang W; Suzuki H; Xuan X
[Ad] Endereço:National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, 080-8555, Japan.
[Ti] Título:Molecular detection and genetic diversity of bovine Babesia spp., Theileria orientalis, and Anaplasma marginale in beef cattle in Thailand.
[So] Source:Parasitol Res;116(2):751-762, 2017 Feb.
[Is] ISSN:1432-1955
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Babesia spp., Theileria orientalis, and Anaplasma marginale are significant tick-borne pathogens that affect the health and productivity of cattle in tropical and subtropical areas. In this study, we used PCR to detect the presence of Babesia bovis, Babesia bigemina, and T. orientalis in 279 beef cattle from Western Thailand and A. marginale in 608 beef cattle from the north, northeastern, and western regions. The PCRs were performed using species-specific primers based on the B. bovis spherical body protein 2 (BboSBP2), B. bigemina rhoptry-associated protein 1a (BbiRAP-1a), T. orientalis major piroplasm surface protein (ToMPSP), and A. marginale major surface protein 4 (AmMSP4) genes. To determine the genetic diversity of the above parasites, amplicons of B. bovis and B. bigemina ITS1-5.8s rRNA gene-ITS2 regions (B. bovis ITS, B. bigemina ITS), ToMPSP, and AmMSP4 genes were sequenced for phylogenetic analysis. PCR results revealed that the prevalence of B. bovis, B. bigemina, T. orientalis, and A. marginale in the Western region was 11.1, 12.5, 7.8, and 39.1 %, respectively. Coinfections of two or three parasites were observed in 17.9 % of the animals sampled. The study revealed that the prevalence of A. marginale in the western region was higher than in the north and northeastern regions (7 %). Sequence analysis showed the BboSBP2 gene to be more conserved than B. bovis ITS in the different isolates and, similarly, the BbiRAP-1a was more conserved than B. bigemina ITS. In the phylogenetic analysis, T. orientalis MPSP sequences were classified into types 3, 5, and 7 as previously reported. A. marginale MSP4 gene sequences shared high identity and similarity with each other and clustered with isolates from other countries. This study provides information on the prevalence and genetic diversity of tick-borne pathogens in beef cattle and highlights the need for effective strategies to control these pathogens in Thailand.
[Mh] Termos MeSH primário: Anaplasmose/microbiologia
Babesiose/parasitologia
Doenças dos Bovinos
Variação Genética
Theileriose/parasitologia
[Mh] Termos MeSH secundário: Anaplasma marginale/genética
Anaplasma marginale/isolamento & purificação
Anaplasmose/epidemiologia
Animais
Babesia/genética
Babesia/isolamento & purificação
Babesia bovis/genética
Babesia bovis/isolamento & purificação
Babesiose/epidemiologia
Sequência de Bases
Bovinos
Doenças dos Bovinos/microbiologia
Doenças dos Bovinos/parasitologia
Primers do DNA/genética
DNA de Protozoário/química
DNA de Protozoário/genética
Geografia
Filogenia
Reação em Cadeia da Polimerase/veterinária
Alinhamento de Sequência/veterinária
Análise de Sequência de DNA/veterinária
Tailândia/epidemiologia
Theileria/genética
Theileria/isolamento & purificação
Theileriose/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Primers); 0 (DNA, Protozoan)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171007
[Lr] Data última revisão:
171007
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161229
[St] Status:MEDLINE
[do] DOI:10.1007/s00436-016-5345-2


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[PMID]:27942962
[Au] Autor:Zhao S; Liu J; Zhao H; Li Y; Xie J; Liu A; Hassan MA; Yin H; Guan G; Luo J
[Ad] Endereço:State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu, 730046, People's Republic of China.
[Ti] Título:Evaluating an indirect rMPSP enzyme-linked immunosorbent assay for the detection of bovine Theileria infection in China.
[So] Source:Parasitol Res;116(2):667-676, 2017 Feb.
[Is] ISSN:1432-1955
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Bovine theileriosis, a tick-borne protozoan disease caused by Theileria annulata, Theileria orientalis and Theileria sinensis, is widespread in China and is a serious economic problem for the Chinese livestock industry. In this study, recombinant major piroplasma surface proteins (MPSP) of T. annulata, T. orientalis and T. sinensis based on MPSP genes were expressed in Escherichia coli BL21(DE3). The immunogenicity and specificity of the three purified recombinant MPSP proteins were evaluated with the reference positive sera of T. annulata, T. orientalis, T. sinensis, Babesia bovis, B abesia bigemina, Babesia major, Babesia motasi, Theileria luwenshuni, Theileria uilenbergi and Anaplasma ovis using an enzyme-linked immunosorbent assay (ELISA) or western blotting. The results showed that all three of the rMPSP proteins had a strong reaction with the sera from cattle infected with T. annulata, T. orientalis and T. sinensis via western blotting but not with other piroplasma and Anaplasma species. Then, the rMPSP protein of T. sinensis was used to develop an iELISA for detecting the three Theileria species infections. The specificity and sensitivity were 95.7 and 95.5 %, respectively, with a threshold of 28.8 % of the specific mean antibody rate (AbR). Finally, 2473 field-collected bovine sera, from 42 prefectures of 17 provinces in China, were tested using the ELISA to evaluate the prevalence of bovine theileriosis, and the average positive rate was 43.6 %. The developed iELISA could be a suitable tool to detect the three bovine Theileria species, and the data also provided important information regarding the current prevalence of bovine theileriosis in China.
[Mh] Termos MeSH primário: Babesia bovis/genética
Doenças dos Bovinos/diagnóstico
Ensaio de Imunoadsorção Enzimática/métodos
Ensaio de Imunoadsorção Enzimática/veterinária
Proteínas de Membrana/análise
Theileria annulata/genética
Theileriose/diagnóstico
[Mh] Termos MeSH secundário: Animais
Babesia bovis/isolamento & purificação
Babesiose/diagnóstico
Babesiose/parasitologia
Western Blotting
Bovinos/parasitologia
Doenças dos Bovinos/epidemiologia
China/epidemiologia
Proteínas Recombinantes
Sensibilidade e Especificidade
Theileria annulata/isolamento & purificação
Theileriose/classificação
Theileriose/epidemiologia
Doenças Transmitidas por Carrapatos/parasitologia
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Membrane Proteins); 0 (Recombinant Proteins)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171007
[Lr] Data última revisão:
171007
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161213
[St] Status:MEDLINE
[do] DOI:10.1007/s00436-016-5332-7


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[PMID]:27911903
[Au] Autor:Oldiges DP; Laughery JM; Tagliari NJ; Leite Filho RV; Davis WC; da Silva Vaz I; Termignoni C; Knowles DP; Suarez CE
[Ad] Endereço:Centro de Biotecnologia Universidade Federal do Rio Grande do Sul, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
[Ti] Título:Transfected Babesia bovis Expressing a Tick GST as a Live Vector Vaccine.
[So] Source:PLoS Negl Trop Dis;10(12):e0005152, 2016 Dec.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The Rhipicephalus microplus tick is a notorious blood-feeding ectoparasite of livestock, especially cattle, responsible for massive losses in animal production. It is the main vector for transmission of pathogenic bacteria and parasites, including Babesia bovis, an intraerythrocytic apicomplexan protozoan parasite responsible for bovine Babesiosis. This study describes the development and testing of a live B. bovis vaccine expressing the protective tick antigen glutathione-S-transferase from Haemaphysalis longicornis (HlGST). The B. bovis S74-T3B parasites were electroporated with a plasmid containing the bidirectional Ef-1α (elongation factor 1 alpha) promoter of B. bovis controlling expression of two independent genes, the selectable marker GFP-BSD (green fluorescent protein-blasticidin deaminase), and HlGST fused to the MSA-1 (merozoite surface antigen 1) signal peptide from B. bovis. Electroporation followed by blasticidin selection resulted in the emergence of a mixed B. bovis transfected line (termed HlGST) in in vitro cultures, containing parasites with distinct patterns of insertion of both exogenous genes, either in or outside the Ef-1α locus. A B. bovis clonal line termed HlGST-Cln expressing intracellular GFP and HlGST in the surface of merozoites was then derived from the mixed parasite line HlGST using a fluorescent activated cell sorter. Two independent calf immunization trials were performed via intravenous inoculation of the HlGST-Cln and a previously described control consisting of an irrelevant transfected clonal line of B. bovis designated GFP-Cln. The control GFP-Cln line contains a copy of the GFP-BSD gene inserted into the Ef-1α locus of B. bovis in an identical fashion as the HIGST-Cln parasites. All animals inoculated with the HlGST-Cln and GFP-Cln transfected parasites developed mild babesiosis. Tick egg fertility and fully engorged female tick weight was reduced significantly in R. microplus feeding on HlGST-Cln-immunized calves. Collectively, these data show the efficacy of a transfected HlGST-Cln B. bovis parasite to induce detectable anti-glutathione-S-transferase antibodies and a reduction in tick size and fecundity of R. microplus feeding in experimentally inoculated animals.
[Mh] Termos MeSH primário: Proteínas de Artrópodes/genética
Babesia bovis/genética
Babesiose/imunologia
Doenças dos Bovinos/prevenção & controle
Glutationa Transferase/genética
Ixodidae/enzimologia
[Mh] Termos MeSH secundário: Animais
Proteínas de Artrópodes/imunologia
Babesia bovis/imunologia
Babesiose/parasitologia
Babesiose/prevenção & controle
Babesiose/transmissão
Bovinos
Doenças dos Bovinos/imunologia
Doenças dos Bovinos/parasitologia
Feminino
Glutationa Transferase/imunologia
Proteínas de Fluorescência Verde/genética
Masculino
Rhipicephalus/parasitologia
Transfecção
Vacinas/genética
Vacinas/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arthropod Proteins); 0 (Vaccines); 147336-22-9 (Green Fluorescent Proteins); EC 2.5.1.18 (Glutathione Transferase)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170613
[Lr] Data última revisão:
170613
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161203
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005152


  9 / 481 MEDLINE  
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[PMID]:27832060
[Au] Autor:Alzan HF; Knowles DP; Suarez CE
[Ad] Endereço:Parasitology and Animal Diseases Department, National Research Center, Dokki, Giza, Egypt.
[Ti] Título:Comparative Bioinformatics Analysis of Transcription Factor Genes Indicates Conservation of Key Regulatory Domains among Babesia bovis, Babesia microti, and Theileria equi.
[So] Source:PLoS Negl Trop Dis;10(11):e0004983, 2016 Nov.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Apicomplexa tick-borne hemoparasites, including Babesia bovis, Babesia microti, and Theileria equi are responsible for bovine and human babesiosis and equine theileriosis, respectively. These parasites of vast medical, epidemiological, and economic impact have complex life cycles in their vertebrate and tick hosts. Large gaps in knowledge concerning the mechanisms used by these parasites for gene regulation remain. Regulatory genes coding for DNA binding proteins such as members of the Api-AP2, HMG, and Myb families are known to play crucial roles as transcription factors. Although the repertoire of Api-AP2 has been defined and a HMG gene was previously identified in the B. bovis genome, these regulatory genes have not been described in detail in B. microti and T. equi. In this study, comparative bioinformatics was used to: (i) identify and map genes encoding for these transcription factors among three parasites' genomes; (ii) identify a previously unreported HMG gene in B. microti; (iii) define a repertoire of eight conserved Myb genes; and (iv) identify AP2 correlates among B. bovis and the better-studied Plasmodium parasites. Searching the available transcriptome of B. bovis defined patterns of transcription of these three gene families in B. bovis erythrocyte stage parasites. Sequence comparisons show conservation of functional domains and general architecture in the AP2, Myb, and HMG proteins, which may be significant for the regulation of common critical parasite life cycle transitions in B. bovis, B. microti, and T. equi. A detailed understanding of the role of gene families encoding DNA binding proteins will provide new tools for unraveling regulatory mechanisms involved in B. bovis, B. microti, and T. equi life cycles and environmental adaptive responses and potentially contributes to the development of novel convergent strategies for improved control of babesiosis and equine piroplasmosis.
[Mh] Termos MeSH primário: Babesia bovis/genética
Babesia microti/genética
Regulação da Expressão Gênica
Genoma de Protozoário
Theileria/genética
Fatores de Transcrição/genética
[Mh] Termos MeSH secundário: Animais
Babesiose/parasitologia
Sequência de Bases
Bovinos
Biologia Computacional/métodos
Proteínas de Ligação a DNA/genética
Perfilação da Expressão Gênica
Doenças dos Cavalos/parasitologia
Cavalos/parasitologia
Seres Humanos
Estágios do Ciclo de Vida/genética
Filogenia
Análise de Sequência de DNA
Theileriose/parasitologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (DNA-Binding Proteins); 0 (Transcription Factors)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170605
[Lr] Data última revisão:
170605
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161111
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0004983


  10 / 481 MEDLINE  
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[PMID]:27784333
[Au] Autor:Guan G; Korhonen PK; Young ND; Koehler AV; Wang T; Li Y; Liu Z; Luo J; Yin H; Gasser RB
[Ad] Endereço:State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, China.
[Ti] Título:Genomic resources for a unique, low-virulence Babesia taxon from China.
[So] Source:Parasit Vectors;9(1):564, 2016 Oct 27.
[Is] ISSN:1756-3305
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Babesiosis is a socioeconomically important tick-borne disease of animals (including humans) caused by haemoprotozoan parasites. The severity of babesiosis relates to host and parasite factors, particularly virulence/pathogenicity. Although Babesia bovis is a particularly pathogenic species of cattle, there are species of Babesia of ruminants that have limited pathogenicity. For instance, the operational taxonomic unit Babesia sp. Xinjiang (abbreviated here as Bx) of sheep from China is substantially less virulent/pathogenic than B. bovis is in cattle. Although the reason for this distinctiveness is presently unknown, it is possible that Bx has a reduced ability to adhere to cells or evade/suppress immune responses, which might relate to particular proteins, such as the variant erythrocyte surface antigens (VESAs). RESULTS: We sequenced and annotated the 8.4 Mb nuclear draft genome of Bx and compared it with those of B. bovis and B. bigemina by synteny analysis; we also investigated the genetic relationship of Bx with selected Babesia species and related apicomplexans for which genomic datasets are available, and explored the VESA complement in Bx. CONCLUSIONS: The availability of the Bx genome now provides unique opportunities to elucidate aspects of the molecular biology, biochemistry and physiology of Bx, and to explore the reason(s) for its limited virulence and/or apparent ability to evade immune attack by the host animal. Moreover, the present genomic resource and an in vitro culture system for Bx raises the prospect of establishing a functional genomic platform to explore essential genes as new intervention targets against babesiosis.
[Mh] Termos MeSH primário: Babesia/classificação
Babesia/genética
Genoma de Protozoário
[Mh] Termos MeSH secundário: Animais
Babesia/isolamento & purificação
Babesia/patogenicidade
Babesia bovis/genética
Babesiose/epidemiologia
Babesiose/parasitologia
Bovinos
Doenças dos Bovinos/epidemiologia
Doenças dos Bovinos/parasitologia
China/epidemiologia
DNA de Protozoário
Anotação de Sequência Molecular
Filogenia
Reação em Cadeia da Polimerase
Análise de Sequência de DNA
Ovinos
Doenças dos Ovinos/epidemiologia
Doenças dos Ovinos/parasitologia
Sintenia
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Protozoan)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171025
[Lr] Data última revisão:
171025
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161028
[St] Status:MEDLINE



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