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Pesquisa : B01.043.075.600.580.700 [Categoria DeCS]
Referências encontradas : 722 [refinar]
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[PMID]:28832147
[Au] Autor:Badral D; Odonbayar B; Murata T; Munkhjargal T; Tuvshintulga B; Igarashi I; Suganuma K; Inoue N; Brantner AH; Odontuya G; Sasaki K; Batkhuu J
[Ad] Endereço:School of Engineering and Applied Sciences, National University of Mongolia , Ulaanbaatar 14201, Mongolia.
[Ti] Título:Flavonoid and Galloyl Glycosides Isolated from Saxifraga spinulosa and Their Antioxidative and Inhibitory Activities against Species That Cause Piroplasmosis.
[So] Source:J Nat Prod;80(9):2416-2423, 2017 Sep 22.
[Is] ISSN:1520-6025
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Eight new flavonoid-based 3'-O-ß-d-glucopyranosides (1-8) and three new galloyl glucosides (9, 11, 12), were isolated from the aerial parts of Saxifraga spinulosa, along with 25 known compounds. The structures of the new compounds were elucidated by spectroscopic methods. Most of the isolated compounds exhibited potent DPPH radical-scavenging activities. Further, their inhibitory activities were evaluated against Babesia bovis, Babesia bigemina, Babesia caballi, and Theileria equi, protozoan parasites that cause piroplasmosis in livestock. The results indicated that several of these compounds showed growth-inhibitory effects on such organisms that cause piroplasmosis.
[Mh] Termos MeSH primário: Antioxidantes/farmacologia
Babesia/química
Flavonoides/farmacologia
Glicosídeos/farmacologia
Saxifragaceae/química
Theileria/química
[Mh] Termos MeSH secundário: Animais
Antioxidantes/química
Flavonoides/química
Glicosídeos/química
Estrutura Molecular
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Flavonoids); 0 (Glycosides)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170824
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jnatprod.7b00142


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[PMID]:28655583
[Au] Autor:Rizk MA; El-Sayed SAE; AbouLaila M; Yokoyama N; Igarashi I
[Ad] Endereço:National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-Cho, Obihiro, Hokkaido, Japan; Department of Internal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt.
[Ti] Título:Evaluation of the inhibitory effect of N-acetyl-L-cysteine on Babesia and Theileria parasites.
[So] Source:Exp Parasitol;179:43-48, 2017 Aug.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:N-acetyl-L-cysteine is known to have antibacterial, antiviral, antimalarial, and antioxidant activities. Therefore, the in vitro inhibitory effect of this hit was evaluated in the present study on the growth of Babesia and Theileria parasites. The in vitro growth of Babesia bovis, Babesia bigemina, Babesia divergens, Theileria equi, and Babesia caballi that were tested was significantly inhibited (P < 0.05) by micromolar concentrations of N-acetyl-L-cysteine. The inhibitory effect of N-acetyl-L-cysteine was synergistically potentiated when used in combination with diminazene aceturate on B. bovis and B. caballi cultures. These results indicate that N-acetyl-L-cysteine might be used as a drug for the treatment of babesiosis, especially when used in combination with diminazene aceturate.
[Mh] Termos MeSH primário: Acetilcisteína/farmacologia
Antiprotozoários/farmacologia
Babesia/efeitos dos fármacos
Diminazena/análogos & derivados
Theileria/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Babesia/crescimento & desenvolvimento
Babesia bovis/efeitos dos fármacos
Babesia bovis/crescimento & desenvolvimento
Bovinos
Diminazena/farmacologia
Sinergismo Farmacológico
Eritrócitos/parasitologia
Cavalos
Concentração Inibidora 50
Espectrometria de Fluorescência
Theileria/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antiprotozoal Agents); JI8SAD85NO (diminazene aceturate); WYQ7N0BPYC (Acetylcysteine); Y5G36EEA5Z (Diminazene)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170726
[Lr] Data última revisão:
170726
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170629
[St] Status:MEDLINE


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[PMID]:28605963
[Au] Autor:Davitkov D; Davitkov D; Vucicevic M; Stanisic L; Radakovic M; Glavinic U; Stanimirovic Z
[Ad] Endereço:Department of Biology, Faculty of Veterinary Medicine, University of Belgrade , Belgrade , Serbia.
[Ti] Título:A molecular and haematological study of Theileria equi in Balkan donkeys.
[So] Source:Acta Vet Hung;65(2):234-241, 2017 06.
[Is] ISSN:0236-6290
[Cp] País de publicação:Hungary
[La] Idioma:eng
[Ab] Resumo:Equine piroplasmosis in donkeys has been recognised as a serious problem of major economic importance. The present molecular study is the first investigation of the presence of Theileria equi and Babesia caballi in Balkan donkeys and of the possible haematological alterations related to it. A total of 70 apparently healthy donkeys from Serbia were included in this study. The overall prevalence of T. equi infection in donkeys tested with multiplex PCR was 50%. There was no B. caballi-positive sample. Infections in donkeys included in this study seem to be associated with decreased red blood cell count, haemoglobin concentration, haematocrit and platelet count, and with increased white blood cell count, mean corpuscular haemoglobin and mean corpuscular haemoglobin concentration. Altered haematological parameters in donkeys can lead to a decrease in working capacity and production performance. Further molecular research and long-term monitoring of equine piroplasmosis is needed in Serbia and throughout Europe.
[Mh] Termos MeSH primário: Equidae/parasitologia
Theileria/classificação
Theileriose/parasitologia
[Mh] Termos MeSH secundário: Animais
Babesiose/epidemiologia
Babesiose/parasitologia
Equidae/sangue
Sérvia/epidemiologia
Estudos Soroepidemiológicos
Theileria/isolamento & purificação
Theileriose/sangue
Theileriose/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170614
[St] Status:MEDLINE
[do] DOI:10.1556/004.2017.023


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[PMID]:28555954
[Au] Autor:Swilks E; Jenkins C; Poynting A; Collins D; Krebs GL
[Ad] Endereço:School of Animal and Veterinary Sciences, Charles Sturt University, Locked Bag 588, Wagga Wagga, New South Wales 2678, Australia.
[Ti] Título:Prevalence and effect of Theileria orientalis infection in homebred calves in the Gloucester region of New South Wales, Australia.
[So] Source:Aust Vet J;95(6):211-216, 2017 Jun.
[Is] ISSN:1751-0813
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: To examine the prevalence of Theileria orientalis in the Gloucester area of New South Wales and its effect on individual animals. METHODS: Blood samples (EDTA and clotted blood) were collected from a total of 55 calves and their dams from 6 properties over a 16-week period. A total of 202 and 190 blood samples were collected from the calves and dams, respectively, and were examined via blood film for the presence of intraerythrocytic T. orientalis piroplasms. Packed cell volume (PCV) was measured to determine infection resulting in anaemia. The presence of antibodies against the T. orientalis major piroplasm surface protein (MPSP) was tested using ELISA. RESULTS: The overall prevalence of T. orientalis infection in both dams and calves from all herds examined was 95%. Mean peak parasitaemia was observed in calves between 6 and 9 weeks of age (P = 0.051), coinciding with a decline in mean PCV. Only 3 (6%) of the blood samples collected from the dams were positive for Theileria-associated antibodies and no significant relationship (P > 0.05) was found between the presence of antibodies in the dams and PCV levels in the calves. There was no evidence that passive transfer of antibodies from dams to calves protected the calves against a decline in PCV. CONCLUSION: This study confirmed a high prevalence of low-level Theileria infection, but low MPSP seroconversion rates, in dams and calves in an area where the disease has been endemic for a number of years.
[Mh] Termos MeSH primário: Doenças dos Bovinos/epidemiologia
Doenças dos Bovinos/parasitologia
Theileriose/epidemiologia
[Mh] Termos MeSH secundário: Anemia/epidemiologia
Anemia/parasitologia
Anemia/veterinária
Animais
Anticorpos Antiprotozoários/sangue
Bovinos
Doenças dos Bovinos/sangue
Ensaio de Imunoadsorção Enzimática/veterinária
Feminino
Masculino
Merozoítos
New South Wales/epidemiologia
Reação em Cadeia da Polimerase/veterinária
Prevalência
Theileria/genética
Theileria/imunologia
Theileriose/sangue
Theileriose/complicações
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Protozoan)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171006
[Lr] Data última revisão:
171006
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170531
[St] Status:MEDLINE
[do] DOI:10.1111/avj.12593


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[PMID]:28495200
[Au] Autor:Ali AM; Salih DA; Njahira MN; Hassan SK; El Hussein AM; Liu Z; Yin H; Pelle R; Skilton RA
[Ad] Endereço:Biosciences eastern and central Africa-International Livestock Research Institute (BecA-ILRI) Hub, P.O. Box 30709-00100, Nairobi, Kenya; University of Khartoum, Faculty of Veterinary Medicine, Khartoum, Sudan. Electronic address: Awadia_mosa@hotmail.com.
[Ti] Título:Genotyping of Theileria lestoquardi from sheep and goats in Sudan to support control of Malignant Ovine Theileriosis.
[So] Source:Vet Parasitol;239:7-14, 2017 May 30.
[Is] ISSN:1873-2550
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Theileriosis, caused by parasitic protozoa of the genus Theileria parasites, are among the major tick-borne diseases of ruminant livestock. The largest economic losses are attributed in particular to those caused by the leukoproliferative species of Theileria: T. parva, T. annulata and T. lestoquardi. Theileria lestoquardi is transmitted by Hyalomma ticks and causes malignant ovine theileriosis (MOT), a disease that is particularly prevalent in Sudan. The disease is considered of a high economic importance in Sudan, where export of sheep is a major component of the national economy. A live vaccine based on a Sudanese isolate of T. lestoquardi (Atbara strain) was previously developed for the control of MOT in Sudan, but not yet deployed in the field. The present study aims to genetically characterize and compare samples of T. lestoquardi circulating in Sudan as well as the live vaccine isolate in order to understand vaccine breakthroughs and failure that may occur. Sheep and goats blood samples were collected from six regions in Sudan that are known to be endemic for T. lestoquardi infection or have experienced outbreaks of MOT. Blood samples infected with T. lestoquardi were identified by PCR or RLB. Genotyping was carried out by (1) sequencing the homologues of two T. parva CD8 T cell antigen genes, Tp1 and Tp2, and (2) using a panel of seven micro- and mini-satellite markers. A total of 100 T. lestoquardi positive field samples and the T. lestoquardi (Atbara) vaccine were genotyped. The results showed that all samples had mixed genotypes, with several alleles identified at one or more loci. The gene diversity ranged from 0.7840 (TS8) to 0.2133 (TS12) with mean values of 0.5470. PCA revealed three clusters of the parasite in Sudan; interestingly one independent cluster was clearly seen, corresponding to the vaccine isolate. The T. lestoquardi Tp1 homologue showed higher homology with T. annulata than with T. parva sequences included the defined single CD8 T cell target epitope region. The result indicates that multiple genotypes are a common feature of T. lestoquardi infection in Sudan. Both genotyping and the sequencing results clearly showed that the vaccine isolate is highly distinct from the field samples. This finding raised the question whether vaccination with the prepared lived vaccine will effectively protect animals against challenges by the field isolates of T. lestoquardi. The results of this work will inform on the best approach for controlling MOT in Sudan.
[Mh] Termos MeSH primário: Doenças das Cabras/parasitologia
Vacinas Protozoárias
Doenças dos Ovinos/parasitologia
Theileria/genética
Theileriose/parasitologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Regulação da Expressão Gênica/fisiologia
Técnicas de Genotipagem/métodos
Técnicas de Genotipagem/veterinária
Doenças das Cabras/epidemiologia
Doenças das Cabras/prevenção & controle
Cabras
Proteínas de Protozoários/genética
Proteínas de Protozoários/metabolismo
Ovinos
Doenças dos Ovinos/epidemiologia
Doenças dos Ovinos/prevenção & controle
Sudão/epidemiologia
Theileria/classificação
Theileria/imunologia
Theileriose/epidemiologia
Theileriose/prevenção & controle
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Protozoan Proteins); 0 (Protozoan Vaccines)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170513
[St] Status:MEDLINE


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[PMID]:28411879
[Au] Autor:Latré de Laté P; Pineda M; Harnett M; Harnett W; Besteiro S; Langsley G
[Ad] Endereço:Inserm U1016, Cnrs UMR8104, Cochin Institute, Paris, France; Comparative Cellbiology of Apicomplexan Parasites, Faculty of Medicine, Paris-Descartes University, Paris, France.
[Ti] Título:Apicomplexan autophagy and modulation of autophagy in parasite-infected host cells.
[So] Source:Biomed J;40(1):23-30, 2017 02.
[Is] ISSN:2320-2890
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Apicomplexan parasites are responsible for a number of important human pathologies. Obviously, as Eukaryotes they share a number of cellular features and pathways with their respective host cells. One of them is autophagy, a process involved in the degradation of the cell's own components. These intracellular parasites nonetheless seem to present a number of original features compared to their very evolutionarily distant host cells. In mammals and other metazoans, autophagy has been identified as an important contributor to the defence against microbial pathogens. Thus, host autophagy also likely plays a key role in the control of apicomplexan parasites, although its potential manipulation and subversion by intracellular parasites creates a complex interplay in the regulation of host and parasite autophagy. In this mini-review, we summarise current knowledge on autophagy in both parasites and their host cells, in the context of infection by three Apicomplexa: Plasmodium, Toxoplasma, and Theileria.
[Mh] Termos MeSH primário: Autofagia/fisiologia
Interações Hospedeiro-Parasita/fisiologia
Parasitos/isolamento & purificação
Plasmodium/microbiologia
[Mh] Termos MeSH secundário: Animais
Seres Humanos
Theileria/microbiologia
Toxoplasma/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171005
[Lr] Data última revisão:
171005
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170417
[St] Status:MEDLINE


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[PMID]:28355109
[Au] Autor:Abdallah MO; Niu Q; Yang J; Hassan MA; Yu P; Guan G; Chen Z; Liu G; Luo J; Yin H
[Ad] Endereço:State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Xujiaping 1, Lanzhou, Gansu, 730046, P. R. China. Correspondence should be sent to Hong Yin at: yinhong
[Ti] Título:Identification of 12 Piroplasms Infecting Ten Tick Species in China Using Reverse Line Blot Hybridization.
[So] Source:J Parasitol;103(3):221-227, 2017 Jun.
[Is] ISSN:1937-2345
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Piroplasmosis, a disease of domestic and wild animals, is caused by tick-borne protozoa in the genera of Theileria and Babesia. There is limited information available about the prevalence of piroplasmosis in ticks in China, and to assess the potential threat of piroplasmosis in China, we investigated the infections of ovine and bovine Babesia and Theileria species in ticks collected from cattle, yaks, sheep, horses, and camels in several regions of China where tick-borne diseases have been reported. In total, 652 ticks were collected from the animals in 6 provinces of China. Babesia spp. and Theileria spp. were detected with a PCR-RLB method and identified by sequencing. Overall, 157 ticks (24.1%) were infected with 5 Babesia and 4 Theileria species. Among tested tick samples, 134 (20.6%) were single infections with 1 of 7 piroplasm species, with Theileria annulata (118/652, 18.1%) being dominant. Only 23 (3.5%) tick samples were double or triple infected, Theileria luwenshuni and Theileria sinensis (18/652, 2.8%) were frequently observed in co-infections. Some piroplasm species were carried by ticks that were not previously reported to be vectors.
[Mh] Termos MeSH primário: Babesia/isolamento & purificação
Theileria/isolamento & purificação
Carrapatos/parasitologia
[Mh] Termos MeSH secundário: Animais
Babesia/classificação
Babesia/genética
Camelus
Bovinos
China
Cavalos
Reação em Cadeia da Polimerase/métodos
Reação em Cadeia da Polimerase/veterinária
Prevalência
RNA Ribossômico 18S/genética
Ovinos
Theileria/classificação
Theileria/genética
Theileria annulata/classificação
Theileria annulata/genética
Theileria annulata/isolamento & purificação
Infestações por Carrapato/parasitologia
Infestações por Carrapato/veterinária
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 18S)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170628
[Lr] Data última revisão:
170628
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170330
[St] Status:MEDLINE
[do] DOI:10.1645/16-161


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[PMID]:28288762
[Au] Autor:Zanet S; Bassano M; Trisciuoglio A; Taricco I; Ferroglio E
[Ad] Endereço:University of Turin, Dept. of Veterinary Sciences, Largo Braccini 2, 10095 Grugliasco, To, Italy.
[Ti] Título:Horses infected by Piroplasms different from Babesia caballi and Theileria equi: species identification and risk factors analysis in Italy.
[So] Source:Vet Parasitol;236:38-41, 2017 Mar 15.
[Is] ISSN:1873-2550
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Equine Piroplasmosis (EP) caused by Theileria equi and Babesia caballi is a disease affecting the health and the international movement of horses. In order to assess prevalence of Piroplasmid infection in the Northwestern part of Italy and to evaluate the associated risk factors, whole blood was collected from 135 horses from 7 different stables across the study area. PCR and sequencing were used to assess prevalence of infection and to identify detected Piroplasms to species level. A total of 23 horses (P=17.04%; CI95%: 10.70-23.38%) was found to be infected with Piroplasms and T. equi was the most prevalent species, found in 18 animals (P=13.33%; CI95%: 7.60%-19.07%). Although B. caballi was never detected, the presence of parasites belonging to the genus Babesia was confirmed by sequencing in 5 horses, 3 of which were infected with B. canis (P=2.22%; CI95% 0.76%-6.33%), and 2 with B. capreoli (P=1.48%; CI95% 0.41%-5.24%). The natural reservoir hosts of B. canis and B. capreoli are the domestic dog and roe deer Capreolus capreolus respectively. These findings pose attention to the need of considering in future epidemiological and clinical studies, other Apicomplexan species as able to infect horses.
[Mh] Termos MeSH primário: Babesia/isolamento & purificação
Babesiose/epidemiologia
Doenças dos Cavalos/epidemiologia
Doenças dos Cavalos/parasitologia
Theileria/isolamento & purificação
Theileriose/epidemiologia
[Mh] Termos MeSH secundário: Animais
Babesia/classificação
Babesia/genética
Babesiose/parasitologia
DNA de Protozoário/genética
Feminino
Cavalos
Itália/epidemiologia
Masculino
Filogenia
Prevalência
RNA Ribossômico 18S/genética
Fatores de Risco
Análise de Sequência de DNA/veterinária
Theileria/classificação
Theileria/genética
Theileriose/parasitologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Protozoan); 0 (RNA, Ribosomal, 18S)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170908
[Lr] Data última revisão:
170908
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170315
[St] Status:MEDLINE


  9 / 722 MEDLINE  
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[PMID]:28286324
[Au] Autor:Kamyingkird K; Cao S; Tuvshintulga B; Salama A; Mousa AA; Efstratiou A; Nishikawa Y; Yokoyama N; Igarashi I; Xuan X
[Ad] Endereço:National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, 080-8555, Japan; Department of Parasitology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, 10900, Thailand.
[Ti] Título:Effects of dihydroorotate dehydrogenase (DHODH) inhibitors on the growth of Theileria equi and Babesia caballi in vitro.
[So] Source:Exp Parasitol;176:59-65, 2017 May.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Theileria equi and Babesia caballi are the causative agents of equine piroplasmosis (EP), which affects equine production in various parts of the world. However, a safe and effective drug is not currently available for treatment of EP. Dihydroorotate dehydrogenase (DHODH) is the fourth enzyme in the de novo pyrimidine synthesis pathway and has been known as a novel drug target for several apicomplexan protozoan parasites. In this study, we evaluated four DHODH inhibitors; atovaquone (ATV), leflunomide (LFN), brequinar (Breq), and 7-hydroxy-5-[1,2,4] triazolo [1,5,a] pyrimidine (TAZ) on the growth of T. equi and B. caballi in vitro and compared them to diminacene aceturate (Di) as the control drug. The growth of T. equi and B. caballi was significantly hindered by all inhibitors except TAZ. The half maximal inhibitory concentration (IC ) of ATV, LFN, Breq and Di against T. equi was approximately 0.028, 109, 11 and 40 µM, respectively, whereas the IC of ATV, LFN, Breq and Di against B. caballi was approximately 0.128, 193, 5.2 and 16.2 µM, respectively. Using bioinformatics and Western blot analysis, we showed that TeDHODH was similar to other Babesia parasite DHODHs, and confirmed that targeting DHODHs could be useful for the development of novel chemotherapeutics for treatment of EP.
[Mh] Termos MeSH primário: Babesia/efeitos dos fármacos
Inibidores Enzimáticos/farmacologia
Oxirredutases atuantes sobre Doadores de Grupo CH-CH/antagonistas & inibidores
Theileria/efeitos dos fármacos
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Antiprotozoários/farmacologia
Atovaquona/farmacologia
Babesia/classificação
Babesia/crescimento & desenvolvimento
Babesiose/tratamento farmacológico
Babesiose/parasitologia
Compostos de Bifenilo/farmacologia
Biologia Computacional
Diminazena/análogos & derivados
Diminazena/farmacologia
Inibidores Enzimáticos/uso terapêutico
Doenças dos Cavalos/tratamento farmacológico
Doenças dos Cavalos/parasitologia
Cavalos
Concentração Inibidora 50
Isoxazóis/farmacologia
Camundongos
Peso Molecular
Oxirredutases atuantes sobre Doadores de Grupo CH-CH/química
Filogenia
Plasmodium berghei/efeitos dos fármacos
Plasmodium berghei/crescimento & desenvolvimento
Pirimidinas/farmacologia
Pirimidinas/uso terapêutico
Theileria/classificação
Theileria/crescimento & desenvolvimento
Theileriose/tratamento farmacológico
Theileriose/parasitologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antiprotozoal Agents); 0 (Biphenyl Compounds); 0 (Enzyme Inhibitors); 0 (Isoxazoles); 0 (Pyrimidines); 5XL19F49H6 (brequinar); EC 1.3.- (Oxidoreductases Acting on CH-CH Group Donors); EC 1.3.5.2 (dihydroorotate dehydrogenase); G162GK9U4W (leflunomide); JI8SAD85NO (diminazene aceturate); K8CXK5Q32L (pyrimidine); Y5G36EEA5Z (Diminazene); Y883P1Z2LT (Atovaquone)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170411
[Lr] Data última revisão:
170411
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170314
[St] Status:MEDLINE


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[PMID]:28169066
[Au] Autor:Sudan V; Shanker D; Jaiswal A; Singh A; Pandey V
[Ad] Endereço:College of Veterinary Sciences and Animal Husbandry, UP Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura 281001, India. Electronic address: viks.sudan@gmail.com.
[Ti] Título:Standardization and validation of simple PCR, duplex PCR and RAPD in comparison to blood smear examination for diagnosing bovine tropical theileriosis.
[So] Source:Biologicals;46:88-91, 2017 Mar.
[Is] ISSN:1095-8320
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Bovine Tropical Theileriosis (BTT) is an important vector-borne protozoan disease that imposing serious constraints on the health and productivity of domestic cattle. It is matter of common fact that following recovery from primary infection, cattle become persistent carriers and act as reservoirs of infection thereby, playing a critical role in disease epidemiology. The present study describes the comparative diagnostic efficiency of simplex PCR, duplex PCR and RAPD assays for detection of Theileria annulata in cattle. An optimized simple PCR and duplex PCR assay were established using TAMS F/R as primer sets encoding for 721 bp amplicon alongside a RAPD with arbitrary primer coding for 963 bp product of T. annulata. The simple PCR and duplex PCR detected pathogen with almost same level of sensitivity, irrespective of whether its DNA was amplified in isolation or together with DNA of another pathogen without nonspecific amplifications. RAPD failed to give comparable results and suffered from limitations of sensitivity as well as specificity. The developed assays may be seen as a good tool for epidemiological studies aiming at assessing the burden of chronic infections and improving control of the associated diseases in endemic regions.
[Mh] Termos MeSH primário: Doenças dos Bovinos/diagnóstico
Reação em Cadeia da Polimerase/métodos
Técnica de Amplificação ao Acaso de DNA Polimórfico/métodos
Theileria/genética
Theileriose/diagnóstico
[Mh] Termos MeSH secundário: Animais
Bovinos
Doenças dos Bovinos/sangue
Doenças dos Bovinos/parasitologia
DNA de Protozoário/genética
Eletroforese em Gel de Ágar
Reação em Cadeia da Polimerase/normas
Técnica de Amplificação ao Acaso de DNA Polimórfico/normas
Padrões de Referência
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
Theileria/fisiologia
Theileriose/sangue
Theileriose/parasitologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (DNA, Protozoan)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170418
[Lr] Data última revisão:
170418
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170208
[St] Status:MEDLINE



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