Base de dados : MEDLINE
Pesquisa : B01.043.185.650.375 [Categoria DeCS]
Referências encontradas : 130 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 13 ir para página                         

  1 / 130 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28528516
[Au] Autor:Fu YW; Wang B; Zhang QZ; Xu DH; Lin DJ; Yang XY; Zhu SQ; Pan JY; Deng Q; Liu YM; Zhou SY
[Ad] Endereço:Institute of Hydrobiology, Engineering Research Center of Tropical and Subtropical Aquatic Ecological Engineering Ministry of Education, Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, Jinan University, Guangzhou, 510632, People's Republic of China.
[Ti] Título:Combined effects of Chinese medicine feed and ginger extract bath on co-infection of Ichthyophthirius multifiliis and Dactylogyrus ctenopharyngodonid in grass carp.
[So] Source:Parasitol Res;116(7):2017-2025, 2017 Jul.
[Is] ISSN:1432-1955
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Dactylogyrus ctenopharyngodonid and Ichthyophthirius multifiliis are two important ectoparasites of freshwater fish. Co-infection by the two parasites leads to high fish mortality and results in heavy economic losses. This study aimed to evaluate the efficacy of medicated feed and a ginger extract bath against D. ctenopharyngodonid and I. multifiliis on grass carp and investigate the hematological response of grass carp co-infected by the two parasites. These results demonstrated that red blood cell (RBC) and thrombocyte percentage among leucocytes significantly decreased after grass carp were co-infected by D. ctenopharyngodonid and I. multifiliis. The monocyte and neutrophil percentages significantly increased with the increment of parasite mean intensities, while the lymphocyte percentage decreased. The activities of serum acid phosphatase (ACP), alkaline phosphatase (AKP), lysozyme (LZM), and superoxide dismutase (SOD) significantly increased after co-infection. When grass carp treated with medicated feed containing 4% of Astragalus membranaceus, Allium sativum, Morus alba, and Glycyrrhiza uralensis, the activities of ACP, AKP, LZM, and SOD were significantly enhanced, and the mean intensities of D. ctenopharyngodonid and I. multifiliis were significantly decreased. When grass carp was treated with medicated feed and a 4-mg/L ginger extract bath, all parasites were eliminated during 28 days. The bath of ginger extract at a concentration of 4 mg/L kept a low mean intensity of I. multifiliis and D. ctenopharyngodonid, then the two parasites were eliminated by oral administration of the medicated feed with an immunostimulant (Chinese medicine compound).
[Mh] Termos MeSH primário: Carpas/parasitologia
Infecções por Cilióforos/veterinária
Medicamentos de Ervas Chinesas/uso terapêutico
Doenças dos Peixes/parasitologia
Hymenostomatida
Infecções por Trematódeos/veterinária
[Mh] Termos MeSH secundário: Ração Animal
Animais
Infecções por Cilióforos/tratamento farmacológico
Coinfecção
Alho
Gengibre
Hymenostomatida/efeitos dos fármacos
Trematódeos
Infecções por Trematódeos/tratamento farmacológico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Drugs, Chinese Herbal)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171101
[Lr] Data última revisão:
171101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170522
[St] Status:MEDLINE
[do] DOI:10.1007/s00436-017-5507-x


  2 / 130 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28428147
[Au] Autor:Chi H; Taik P; Foley EJ; Racicot AC; Gray HM; Guzzetta KE; Lin HY; Song YL; Tung CH; Zenke K; Yoshinaga T; Cheng CY; Chang WJ; Gong H
[Ad] Endereço:Biotechnology Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China.
[Ti] Título:High genetic diversities between isolates of the fish parasite Cryptocaryon irritans (Ciliophora) suggest multiple cryptic species.
[So] Source:Mol Phylogenet Evol;112:47-52, 2017 Jul.
[Is] ISSN:1095-9513
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The ciliate protozoan Cryptocaryon irritans parasitizes marine fish and causes lethal white spot disease. Sporadic infections as well as large-scale outbreaks have been reported globally and the parasite's broad host range poses particular threat to the aquaculture and ornamental fish markets. In order to better understand C. irritans' population structure, we sequenced and compared mitochondrial cox-1, SSU rRNA, and ITS-1 sequences from 8 new isolates of C. irritans collected in China, Japan, and Taiwan. We detected two SSU rRNA haplotypes, which differ at three positions, separating the isolates into two main groups (I and II). Cox-1 sequences also support the division into two groups, and the cox-1 divergence between these two groups is unexpectedly high (9.28% for 1582 nucleotide positions). The divergence is much greater than that detected in Ichthyophthirius multifiliis, the ciliate protozoan causing freshwater white spot disease in fish, where intraspecies divergence on cox-1 sequence is only 1.95%. ITS-1 sequences derived from these eight isolates and from all other C. irritans isolates (deposited in the GenBank) not only support the two groups, but further suggest the presence of a third group with even greater sequence divergence. Finally, a small Ka/Ks ratio estimated from cox-1 sequences suggests that this gene in C. irritans remains under strong purifying selection. Taken together, the C. irritans species may consists of many subspecies and/or syngens. Further work is needed to determine if there is reproductive isolation between the groups we have defined.
[Mh] Termos MeSH primário: Variação Genética
Hymenostomatida/genética
[Mh] Termos MeSH secundário: Animais
Aquicultura
China
Peixes/parasitologia
Especiação Genética
Japão
Filogenia
Taiwan
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170422
[St] Status:MEDLINE


  3 / 130 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28291600
[Au] Autor:Bastos Gomes G; Miller TL; Vaughan DB; Jerry DR; McCowan C; Bradley TL; Hutson KS
[Ad] Endereço:Marine and Aquaculture Sciences, College of Science and Engineering and Centre for Sustainable Tropical Fisheries and Aquaculture, James Cook University, Townsville, 4811, QLD, Australia. Electronic address: giana.bastosgomes@my.jcu.edu.au.
[Ti] Título:Evidence of multiple species of Chilodonella (Protozoa, Ciliophora) infecting Australian farmed freshwater fishes.
[So] Source:Vet Parasitol;237:8-16, 2017 Apr 15.
[Is] ISSN:1873-2550
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Parasitic Chilodonella species, Chilodonella piscicola and Chilodonella hexasticha, cause considerable economic losses globally to freshwater farmed fish production. Some genetic studies of Chilodonella spp. have indicated that many species within the genus may form cryptic species complexes. To understand the diversity of Chilodonella spp. infecting Australian freshwater farmed fish, specimens were isolated from infected barramundi (Lates calcarifer) and Murray cod (Maccullochella peelii) from fish farms in tropical north Queensland (QLD), temperate Victoria (Vic) and New South Wales (NSW) for genetic and morphological analysis. Parasites were stained and measured for morphological description and comparative phylogenetic analyses were performed using the mitochondrial small subunit (mtSSU) rDNA marker. Morphological analyses revealed four distinct morphotypes of Chilodonella infecting farmed barramundi and Murray Cod. Three putative species were isolated from barramundi (Chilodonella hexasticha, C. acuta and C. uncinata) and one from Murray cod (C. piscicola). However, phylogenetic analyses detected only three distinct genotypes, with the putative C. hexasticha and C. piscicola sharing 100% sequence identity. This suggests that Australian isolates of C. hexasticha and C. piscicola could represent the same species and may exhibit phenotypic plasticity. Further molecular analysis, including isolates from the type localities, should be performed to support or refute the synonymy of these species.
[Mh] Termos MeSH primário: Infecções por Cilióforos/veterinária
Doenças dos Peixes/parasitologia
Hymenostomatida/classificação
[Mh] Termos MeSH secundário: Animais
Infecções por Cilióforos/parasitologia
Coinfecção/veterinária
DNA Mitocondrial/química
DNA Mitocondrial/genética
DNA de Protozoário/química
DNA de Protozoário/genética
DNA Ribossômico/química
DNA Ribossômico/genética
Hymenostomatida/genética
Hymenostomatida/isolamento & purificação
Hymenostomatida/ultraestrutura
New South Wales
Perciformes
Filogenia
Queensland
Vitória
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Mitochondrial); 0 (DNA, Protozoan); 0 (DNA, Ribosomal)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170315
[St] Status:MEDLINE


  4 / 130 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28288756
[Au] Autor:Liu YM; Zhang QZ; Xu DH; Fu YW; Lin DJ; Zhou SY; Li JP
[Ad] Endereço:Key Laboratory of Aquatic Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, Engineering Research Center of Tropical and Subtropical Aquatic Ecological Engineering Ministry of Education, Institute of Hydrobiology, Jinan University, West 601 Huangpu Avenue, Tianhe Distri
[Ti] Título:Antiparasitic efficacy of curcumin from Curcuma longa against Ichthyophthirius multifiliis in grass carp.
[So] Source:Vet Parasitol;236:128-136, 2017 Mar 15.
[Is] ISSN:1873-2550
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Ichthyophthirius multifiliis is a ciliated parasite that elicits great economic losses in aquaculture. In the present study, a polyphenol compound, curcumin, was obtained from the rhizome of Curcuma longa by bioassay-guided isolation based on the efficacy of anti-I. multifiliis theronts. Anti-I. multifiliis efficacy of curcumin was evaluated in vitro and in vivo. Curcumin resulted in 100% mortality of I. multifiliis theronts at a concentration of 1mg/L within 21.7±1.2min and killed all tomonts at 8mg/L within 31.0±1.0min. Curcumin at 4mg/L for 16h exposure can completely terminate the reproduction of tomonts. The pretreatment with curcumin at concentrations of 0.5, 0.25, and 0.125mg/L for 2h significantly reduced the infectivity of I. multifiliis theronts. Curcumin at 4mg/L completely cured the infected grass carp and protected naive fish from I. multifiliis infection after 10days exposure. The 4h median effective concentration (EC ) of curcumin to I. multifiliis theronts and the 5h EC of curcumin to I. multifiliis tomonts were 0.303mg/L and 2.891mg/L, respectively. The 96h median lethal concentration (LC ) of curcumin to grass carp was 56.8mg/L, which was approximately 187.4 times EC of curcumin to theronts and 19.6 times EC of curcumin to tomonts. The results demonstrated that curcumin has the potential to be a safe and effective therapeutant for controlling ichthyophthiriasis in aquaculture.
[Mh] Termos MeSH primário: Antiprotozoários/farmacologia
Carpas
Infecções por Cilióforos/veterinária
Curcumina/farmacologia
Doenças dos Peixes/tratamento farmacológico
Hymenostomatida/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Antiprotozoários/efeitos adversos
Infecções por Cilióforos/tratamento farmacológico
Infecções por Cilióforos/parasitologia
Curcumina/efeitos adversos
Relação Dose-Resposta a Droga
Doenças dos Peixes/parasitologia
Hymenostomatida/fisiologia
Distribuição Aleatória
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antiprotozoal Agents); IT942ZTH98 (Curcumin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170908
[Lr] Data última revisão:
170908
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170315
[St] Status:MEDLINE


  5 / 130 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27914115
[Au] Autor:Pan X; Shi Z; Wang C; Bourland WA; Chen Y; Song W
[Ad] Endereço:College of Life Science and Technology, Harbin Normal University, Harbin, 150025, China.
[Ti] Título:Molecular Phylogeny and Taxonomy of a New Freshwater Hymenostomatid from Northeastern China, with the Establishment of a New Genus Anteglaucoma gen. n. (Protista, Ciliophora, Oligohymenophorea).
[So] Source:J Eukaryot Microbiol;64(5):564-572, 2017 Sep.
[Is] ISSN:1550-7408
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The morphology, infraciliature and SSU rDNA sequence of a new freshwater hymenostomatid ciliate, Anteglaucoma harbinensis gen. nov., spec. nov., collected from a farmland pond in Harbin, China, were investigated. The new genus Anteglaucoma is characterized as follows: small to medium-sized Glaucomidae with oral apparatus in anterior one-third of cell; paroral membrane composed of almost longitudinally arranged dikinetids; three adoral membranelles nearly equal in length and arranged almost longitudinally in parallel; silverline pattern tetrahymenid. The improved diagnosis of family Glaucomidae Corliss 1971 is provided based on the previous and present work. The type species Anteglaucoma harbinensis spec. nov. is defined by having 32-35 somatic kineties; four or five postoral kineties; membranelle 1 and membranelle 2 having five or six kinetosomal rows, membranelle 3 having three kinetosomal rows; single macronuclear nodule; contractile vacuole on average 15% from posterior body end; locomotion characterized by crawling with a rather hectic jerking motion; freshwater habitat. Phylogenetic analyses show that Anteglaucoma clusters in the family Glaucomidae and groups with the genera Glaucoma. The molecular and morphological data indicate that Glaucomidae is related to the family Bromeliophryidae in the phylogenetic trees.
[Mh] Termos MeSH primário: Água Doce/parasitologia
Hymenostomatida/classificação
Análise de Sequência de DNA/métodos
[Mh] Termos MeSH secundário: China
DNA de Protozoário/genética
DNA Ribossômico/genética
Hymenostomatida/genética
Hymenostomatida/ultraestrutura
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Protozoan); 0 (DNA, Ribosomal)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170919
[Lr] Data última revisão:
170919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161204
[St] Status:MEDLINE
[do] DOI:10.1111/jeu.12382


  6 / 130 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27864673
[Au] Autor:Yao JY; Xu Y; Yuan XM; Yin WL; Yang GL; Lin LY; Pan XY; Wang CF; Shen JY
[Ad] Endereço:Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture, Key Laboratory of Fish Health and Nutrition of Zhejiang Province, Zhejiang Institute of Freshwater Fisheries, Huzhou, 313001, China.
[Ti] Título:Proteomic analysis of differentially expressed proteins in the two developmental stages of Ichthyophthirius multifiliis.
[So] Source:Parasitol Res;116(2):637-646, 2017 Feb.
[Is] ISSN:1432-1955
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Ichthyophthirius is a severe disease of farmed freshwater fish caused by the parasitic ciliate Ichthyophthirius multifiliis (Ich). This disease can lead to considerable economic loss, but the protein profiles in different developmental stages of the parasite remain unknown. In the present study, proteins from trophonts and theronts of Ich were identified by isobaric tags for relative and absolute quantitation (iTRAQ). A total of 2300 proteins were identified in the two developmental stages, of which 1520 proteins were differentially expressed. Among them, 84 proteins were uniquely expressed in the theronts stage, while 656 proteins were expressed only in trophonts. The differentially expressed proteins were catalogued (assorted) to various functions of Ich life cycle, including biological process, cellular component, and molecular function that occur at distinct stages. Using a 1.5-fold change in expression as a physiologically significant benchmark, a lot of differentially expressed proteins were reliably quantified by iTRAQ analysis. Two hundred forty upregulated and 57 downregulated proteins in the trophonts stage were identified as compared with theronts. The identified proteins were involved in various functions of the I. multifiliis life cycle, including binding, catalytic activity, structural molecule activity, and transporter activity. Further investigation of the transcriptional levels of periplasmic immunogenic protein, transketolase, zinc finger, isocitrate dehydrogenase, etc., from the different protein profiles using quantitative RT-PCR showed identical results to the iTRAQ analysis. This work provides an effective resource to further our understanding of Ich biology, and lays the groundwork for the identification of potential drug targets and vaccines candidates for the control of this devastating fish pathogen.
[Mh] Termos MeSH primário: Hymenostomatida/crescimento & desenvolvimento
Hymenostomatida/metabolismo
Proteômica/métodos
[Mh] Termos MeSH secundário: Animais
Carpas/parasitologia
Infecções por Cilióforos/parasitologia
Doenças dos Peixes/parasitologia
Regulação da Expressão Gênica
Estágios do Ciclo de Vida
Espectrometria de Massas em Tandem/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171007
[Lr] Data última revisão:
171007
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161120
[St] Status:MEDLINE
[do] DOI:10.1007/s00436-016-5328-3


  7 / 130 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27801984
[Au] Autor:Moreira GS; Shoemaker CA; Zhang D; Xu DH
[Ad] Endereço:Laboratory of Parasitology, College of Animal Science and Food Engineering, University of São Paulo (USP), Pirassununga, Brazil.
[Ti] Título:Expression of immune genes in skin of channel catfish immunized with live theronts of Ichthyophthirius multifiliis.
[So] Source:Parasite Immunol;39(1), 2017 Jan.
[Is] ISSN:1365-3024
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The objective of this study was to evaluate differential expression of innate and adaptive immune genes, including immunoglobulin, immune cell receptor, cytokine, inflammatory protein, toll-like receptors (TLR) and recombination-activating gene (RAG) in skin from channel catfish, Ictalurus punctatus after immunization with live theronts of Ichthyophthirius multifiliis (Ich) by intraperitoneal injection. The immunized catfish showed significantly higher survival rate (95%) than those of mock-immunized control fish (0% survival) after the theront challenge. The gene expression of innate immune system, such as cytokines (IL-1ß type a, IL-1ß type b, IFN-γ, TGF1-ß and TNF-α) and inflammatory proteins (NF-kB and iNOS 2), showed significant upregulation at day 1 (D1) post-immunization. Expression of TLR genes exhibited a rapid increase from hour 4 (h4) to D10 post-immunization. Genes of the adaptive response, such as the cell receptor MHC I, CD8 , CD4 and TCR-α, showed upregulation at D1, D6 and D10. The TCR-ß expression increased rapidly at h4 and remained upregulated until D10. Immunoglobulin IgM upregulation was detected from h4 until D2 while IgD expression was increased from D1 until D10. Rapid upregulation of innate and adaptive immune genes in skin of catfish following live theront vaccination was demonstrated in this study ultimately resulting in significant protection against Ich infection.
[Mh] Termos MeSH primário: Infecções por Cilióforos/veterinária
Doenças dos Peixes/imunologia
Hymenostomatida/imunologia
Ictaluridae/imunologia
Ictaluridae/parasitologia
Pele/imunologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Antiprotozoários/imunologia
Infecções por Cilióforos/imunologia
Doenças dos Peixes/parasitologia
Imunização/veterinária
Imunoglobulina M
NF-kappa B
Fator de Necrose Tumoral alfa
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Protozoan); 0 (Immunoglobulin M); 0 (NF-kappa B); 0 (Tumor Necrosis Factor-alpha)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170912
[Lr] Data última revisão:
170912
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161102
[St] Status:MEDLINE
[do] DOI:10.1111/pim.12397


  8 / 130 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:27726019
[Au] Autor:Wu Z; Ling F; Song C; Chen W; Wang G
[Ad] Endereço:College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, People's Republic of China.
[Ti] Título:Effects of oral administration of whole plants of Artemisia annua on Ichthyophthirius multifiliis and Aeromonas hydrophila after parasitism by I. multifiliis.
[So] Source:Parasitol Res;116(1):91-97, 2017 Jan.
[Is] ISSN:1432-1955
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Since the use of malachite green was banned, it is desirable to search for alternative drug to control Ichthyophthirius multifiliis infection. Moreover, route of administration which is easy to implement and less stressful to fish is important to treat infection of fish with this parasite. It is also important to enhance protective effect against Aeromonas hydrophila infection after parasitism by I. multifiliis. The present study evaluated the protective effect of diet supplemented with Artemisia annua against I. multifiliis and A. hydrophila infection after the parasitism. The results showed that oral administration with A. annua at a concentration of 20 g/kg feed for 45 days can provide strong protection against I. multifiliis infection. The incidence of infestation and mean number of trophonts on fish fins on day 3 post-challenge (15,000 theronts/fish) were significantly reduced compared with the control, and the survival rate of fish during 15-day period was also increased by nearly 30 %. But, this diet provided very limited protection against higher infection dose. Besides, this study demonstrated that this diet enhanced protective effect against A. hydrophila after the fish were infected with I. multifiliis. On the basis of the above results, oral administration with A. annua can be used as a potential strategy for control I. multifiliis infection and increasing fish survival after parasitism by I. multifiliis.
[Mh] Termos MeSH primário: Artemisia annua
Infecções por Cilióforos/veterinária
Doenças dos Peixes/dietoterapia
Infecções por Bactérias Gram-Negativas/veterinária
[Mh] Termos MeSH secundário: Administração Oral
Aeromonas hydrophila/fisiologia
Animais
Infecções por Cilióforos/dietoterapia
Peixes
Infecções por Bactérias Gram-Negativas/dietoterapia
Hymenostomatida/fisiologia
Análise de Sobrevida
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171007
[Lr] Data última revisão:
171007
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161012
[St] Status:MEDLINE


  9 / 130 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27663853
[Au] Autor:Yao JY; Yuan XM; Xu Y; Yin WL; Lin LY; Pan XY; Yang GL; Wang CF; Shen JY
[Ad] Endereço:Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture, Key Laboratory of Fish Health and Nutrition of Zhejiang Province, Zhejiang Institute of Freshwater Fisheries, Huzhou 313001, China. Electronic address: yaojiayun@126.com.
[Ti] Título:Live recombinant Lactococcus lactis vaccine expressing immobilization antigen (i-Ag) for protection against Ichthyophthirius multifiliis in goldfish.
[So] Source:Fish Shellfish Immunol;58:302-308, 2016 Nov.
[Is] ISSN:1095-9947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The parasite Ichthyophthirius multifiliis (Ich) has been reported in various freshwater fishes worldwide and results in severe losses to both food and aquarium fish production. Lactobacillus strains have a number of properties that make them attractive candidates as delivery vehicles for the presentation to the mucosa of compounds with pharmaceutical interest, in particular vaccines. Here, the present study was conducted to evaluate a live recombinant Lactococcus lactis vaccine expressing immobilization antigen (IAG-52X) in protection against I. multifiliis. A 1266 bp gene fragment containing a potential antigenic epitope of the 48 kDa immobilization antigen of I. multifiliis was assembled from six synthetic ohgonucleotides and cloned into pSIP409 and electrotransformed into Lactobacillus plantarum NC8. The recombinant vaccine candidate was then orally fed into goldfish. The expression of immune-related genes: complement component 3 (C3), MHC I, IgM gene in blood from goldfish at different time points after immunization were evaluated. Immunized fish were than challenged with a lethal dose of infectious I. multifiliis. The cumulative mortality and relative percentage survival (RPS) were also determined. Our results showed that the antibody level in the blood and skin of the immunized fish was statistically significant (P < 0.05) in relation to the control groups. Goldfish orally immunized with NC8-pSIP409- IAG-52X had high serum antibody titers that ranged from 32 to 256 after 28d post immunization, while fish fed with NC8-pSIP409 or PBS had no detectable immobilizing antibody response. Expression of IgM, C3, MHC I genes in the group immunized with IAG-52X were significantly (P < 0.05) up regulated as compared with control group, indicating that different immune cells were actively involved in cellular immune response. The results showed that the average survival rate of fish orally immunized with 10 and 10 NC8-pSIP409-IAG-52X was 60% and 50% respectively. Therefore, NC8-pSIP409-IAG-52X could become a promising oral vaccine candidate against I. multifiliis.
[Mh] Termos MeSH primário: Antígenos de Protozoários/imunologia
Infecções por Cilióforos/veterinária
Doenças dos Peixes/prevenção & controle
Carpa Dourada
Hymenostomatida/imunologia
Proteínas de Protozoários/imunologia
Vacinas Protozoárias/imunologia
Vacinação/veterinária
[Mh] Termos MeSH secundário: Animais
Anticorpos Antiprotozoários/sangue
Infecções por Cilióforos/imunologia
Infecções por Cilióforos/parasitologia
Infecções por Cilióforos/prevenção & controle
Doenças dos Peixes/imunologia
Doenças dos Peixes/parasitologia
Imunidade Celular
Lactococcus lactis/genética
Lactococcus lactis/imunologia
Organismos Geneticamente Modificados/genética
Organismos Geneticamente Modificados/imunologia
Vacinas Sintéticas/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Protozoan); 0 (Antigens, Protozoan); 0 (Protozoan Proteins); 0 (Protozoan Vaccines); 0 (Vaccines, Synthetic); 0 (immobilization antigens)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170923
[Lr] Data última revisão:
170923
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160925
[St] Status:MEDLINE


  10 / 130 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27567935
[Au] Autor:von Gersdorff Jørgensen L
[Ad] Endereço:Laboratory of Aquatic Pathobiology, Department of Veterinary Disease Biology, University of Copenhagen, Stigbøjlen 7, DK-1870, Frederiksberg C, Denmark. Electronic address: lvgj@sund.ku.dk.
[Ti] Título:Infection and immunity against Ichthyophthirius multifiliis in zebrafish (Danio rerio).
[So] Source:Fish Shellfish Immunol;57:335-339, 2016 Oct.
[Is] ISSN:1095-9947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Ichthyophthirius multifiliis, causing white spot disease, is a serious pathogen in aquaculture as well as for the ornamental fish industry. In carp, channel catfish and rainbow trout the immune responses against the parasite have been partly elucidated and these species are able to acquire a high level of immunity against the disease. Zebrafish are however, known to be more resilient towards the disease than channel catfish but the pathology and the ability to obtain protection is unknown. In this study a primary infection in the gills of zebrafish was described and the subsequent acquirement of immunity was evaluated. The parasites in the gills induced hyperplasia, an increase of mucus cells, adhesion and shortening of the secondary lamellae, narrowing of water channels and proliferation. The parasite burden was significantly lower in survivor fish and where all naive fish died within 6 days following secondary infection (challenge) only one of the survivor fish died. This study demonstrates that zebrafish are susceptible to I. multifiliis and that gill pathology is similar to the situation observed in rainbow trout. Furthermore, zebrafish are able to acquire immunity against white spot disease and may therefore be a suitable model organism to study innate and protective immunology and host/parasite interactions during I. multifiliis infections.
[Mh] Termos MeSH primário: Infecções por Cilióforos/veterinária
Doenças dos Peixes/imunologia
Hymenostomatida/fisiologia
Imunidade Inata
Peixe-Zebra
[Mh] Termos MeSH secundário: Animais
Infecções por Cilióforos/imunologia
Infecções por Cilióforos/mortalidade
Infecções por Cilióforos/parasitologia
Coinfecção/imunologia
Coinfecção/mortalidade
Coinfecção/parasitologia
Coinfecção/veterinária
Doenças dos Peixes/mortalidade
Doenças dos Peixes/parasitologia
Brânquias/parasitologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160829
[St] Status:MEDLINE



página 1 de 13 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde