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Pesquisa : B01.043.185.650.375.750 [Categoria DeCS]
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  1 / 20 MEDLINE  
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[PMID]:27593699
[Au] Autor:Pritchard JO; Porter AH; Montagnes DJ
[Ad] Endereço:Institute of Integrative Biology, University of Liverpool, BioSciences Building, Crown Street, Liverpool, L69 7ZB, United Kingdom.
[Ti] Título:Did Gause Have a Yeast Infection?
[So] Source:J Eukaryot Microbiol;63(5):552-7, 2016 Sep.
[Is] ISSN:1550-7408
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We planned to develop predator-prey models using Paramecium and yeast, but they have not been empirically examined since work by Gause in the 1930s. Therefore, we evaluated if Paramecium aurelia ingests and grows on eight yeasts. Recognising that it ingested yeasts but could not grow, we assessed if it might grow on other yeasts, by empirically parameterising a predator-prey model that relies on ingestion, not growth. Simulations were compared to P. aurelia-yeast time-series data, from Gause. We hypothesised that if the model simulated predator-prey dynamics that mimicked the original data, then possibly P. aurelia could grow on yeast; simulations did not mimic the original data. Reviewing works by Gause exposed two issues: experiments were undoubtedly contaminated with bacteria, allowing growth on bacteria, not yeast; and the population cycle data cannot be considered a self-sustaining time series, as they were manipulated by adding yeast and ciliates. We conclude that past and future work should not rely on this system, for either empirical or theoretical evaluations. Finally, although we show that P. aurelia, P. caudatum, Euplotes patella, and Blepharisma sp. cannot grow on yeast, Tetrahymena pyriformis and Colpidium striatum can; these may provide models to explore predator-prey dynamics.
[Mh] Termos MeSH primário: Paramecium/fisiologia
Paramecium/patogenicidade
Comportamento Predatório/fisiologia
Leveduras
[Mh] Termos MeSH secundário: Animais
Bactérias
Cilióforos/crescimento & desenvolvimento
Cilióforos/patogenicidade
Cilióforos/fisiologia
Euplotes/crescimento & desenvolvimento
Euplotes/patogenicidade
Modelos Biológicos
Modelos Teóricos
Mortalidade
Paramecium/crescimento & desenvolvimento
Dinâmica Populacional
Tetrahymena pyriformis
Tetrahymenina
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170828
[Lr] Data última revisão:
170828
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160906
[St] Status:MEDLINE
[do] DOI:10.1111/jeu.12299


  2 / 20 MEDLINE  
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[PMID]:23865693
[Au] Autor:Weisse T; Scheffel U; Stadler P; Foissner W
[Ad] Endereço:Research Institute for Limnology, University of Innsbruck, Herzog Odilostrasse 101, Mondsee, 5310, Austria.
[Ti] Título:Functional ecology of the ciliate Glaucomides bromelicola, and comparison with the sympatric species Bromeliothrix metopoides.
[So] Source:J Eukaryot Microbiol;60(6):578-87, 2013 Nov-Dec.
[Is] ISSN:1550-7408
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We investigated the ecology and life strategy of Glaucomides bromelicola (family Bromeliophryidae), a very common ciliate in the reservoirs (tanks) of bromeliads, assessing its response to food quality and quantity and pH. Further, we conducted competition experiments with the frequently coexisting species Bromeliothrix metopoides (family Colpodidae). In contrast to B. metopoides and many other colpodean ciliates, G. bromelicola does not form resting cysts, which jeopardizes this ciliate when its small aquatic habitats dry out. Both species form bactivorous microstomes and flagellate-feeding macrostomes. However, only G. bromelicola has a low feeding threshold and is able to adapt to different protist food. The higher affinity to the local bacterial and flagellate food renders it the superior competitor relative to B. metopoides. Continuous encystment and excystment of the latter may enable stable coexistence of both species in their natural habitat. Both are tolerant to a wide range of pH (4-9). These ciliates appear to be limited to tank bromeliads because they either lack resting cysts and vectors for long distance dispersal (G. bromelicola) and/or have highly specific food requirements (primarily B. metopoides).
[Mh] Termos MeSH primário: Bromelia/parasitologia
Ecologia
Tetrahymenina/fisiologia
Microbiologia da Água
[Mh] Termos MeSH secundário: Comportamento Alimentar
Concentração de Íons de Hidrogênio
Simpatria
Tetrahymenina/crescimento & desenvolvimento
Água/química
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
059QF0KO0R (Water)
[Em] Mês de entrada:1406
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130720
[St] Status:MEDLINE
[do] DOI:10.1111/jeu.12063


  3 / 20 MEDLINE  
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[PMID]:23347342
[Au] Autor:Foissner W
[Ad] Endereço:Department of Organismic Biology, University of Salzburg, Hellbrunnerstrasse 34, A-5020, Salzburg, Austria. wilhelm.foissner@sbg.ac.at
[Ti] Título:Description of Glaucomides bromelicola n. gen., n. sp. (Ciliophora, Tetrahymenida), a macrostome forming inhabitant of bromeliads (Bromeliaceae), including redescriptions of Glaucoma scintillans and G. reniformis.
[So] Source:J Eukaryot Microbiol;60(2):137-57, 2013 Mar-Apr.
[Is] ISSN:1550-7408
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Glaucomides bromelicola n. gen., n. sp. is a tetrahymenid ciliate common in tank bromeliads of Central and South America. The new genus is characterized by having a kinety fragment along the left mouth margin, an unciliated dorsolateral area, a tetrahymenid silverline pattern, and the ability to produce macrostomes when bacterial food is depleted. I provide a detailed description of the microstome and the macrostome morph, using several morphological methods. This showed that G. bromelicola does not belong to the Glaucomidae, but to the Bromeliophryidae. However, various morphological traits are highly similar to those of Glaucoma reniformis and G. scintillans, which are thus redescribed and compared with G. bromelicola. Most differences are inconspicuous, showing that new tetrahymenids must be described very carefully. The morphological and molecular data suggest a common ancestor for Glaucoma and Glaucomides, both performing their own radiation, the former in ordinary limnetic habitats, the latter in tank bromeliads.
[Mh] Termos MeSH primário: Bromeliaceae/parasitologia
Tetrahymenina/classificação
Tetrahymenina/isolamento & purificação
[Mh] Termos MeSH secundário: América Central
Microscopia
América do Sul
Tetrahymenina/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1308
[Cu] Atualização por classe:130304
[Lr] Data última revisão:
130304
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130126
[St] Status:MEDLINE
[do] DOI:10.1111/jeu.12016


  4 / 20 MEDLINE  
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[PMID]:21831194
[Au] Autor:Müller JP; Hauzy C; Hulot FD
[Ad] Endereço:UFR Sciences de la Vie, UPMC Univ Paris 06, Paris, France. jmuller@biologie.ens.fr
[Ti] Título:Ingredients for protist coexistence: competition, endosymbiosis and a pinch of biochemical interactions.
[So] Source:J Anim Ecol;81(1):222-32, 2012 Jan.
[Is] ISSN:1365-2656
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:1. The interaction between mutualism, facilitation or interference and exploitation competition is of major interest as it may govern species coexistence. However, the interplay of these mechanisms has received little attention. This issue dates back to Gause, who experimentally explored competition using protists as a model [Gause, G.F. (1935) Vérifications expérimentales de la théorie mathématique de la lutte pour la vie. Actualités Scientifiques et Industrielles, 277]. He showed the coexistence of Paramecium caudatum with a potentially allelopathic species, Paramecium bursaria. 2. Paramecium bursaria hosts the green algae Chlorella vulgaris. Therefore, P. bursaria may benefit from carbohydrates synthesised by the algae. Studying endosymbiosis with P. bursaria is possible as it can be freed of its endosymbiont. In addition, C. vulgaris is known to produce allelochemicals, and P. bursaria may benefit also from allelopathic compounds. 3. We designed an experiment to separate the effects of resource exploitation, endosymbiosis and allelopathy and to assess their relative importance for the coexistence of P. bursaria with a competitor that exploits the same resource, bacteria. The experiment was repeated with two competitors, Colpidium striatum or Tetrahymena pyriformis. 4. Results show that the presence of the endosymbiont enables the coexistence of competitors, while its loss leads to competitive exclusion. These results are in agreement with predictions based on resource equilibrium density of monocultures (R*) supporting the idea that P. bursaria's endosymbiont is a resource provider for its host. When P. bursaria and T. pyriformis coexist, the density of the latter shows large variation that match the effects of culture medium of P. bursaria. Our experiment suggests these effects are because of biochemicals produced in P. bursaria culture. 5. Our results expose the hidden diversity of mechanisms that underlie competitive interactions. They thus support Gauses's speculation (1935) that allelopathic effects might have been involved in his competition experiments. We discuss how a species engaged both in competition for a resource and in costly interference such as allelopathy may counterbalance these costs with a resource-provider endosymbiont.
[Mh] Termos MeSH primário: Chlorella vulgaris/fisiologia
Cadeia Alimentar
Paramecium/microbiologia
Paramecium/fisiologia
Simbiose
Tetrahymenina/fisiologia
[Mh] Termos MeSH secundário: Bactérias/crescimento & desenvolvimento
Densidade Demográfica
Especificidade da Espécie
Tetrahymena pyriformis/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1204
[Cu] Atualização por classe:111214
[Lr] Data última revisão:
111214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:110812
[St] Status:MEDLINE
[do] DOI:10.1111/j.1365-2656.2011.01894.x


  5 / 20 MEDLINE  
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[PMID]:21226710
[Au] Autor:Jiang L; Joshi H; Flakes SK; Jung Y
[Ad] Endereço:School of Biology, Georgia Institute of Technology, 310 Ferst Drive, Atlanta, GA 30332, USA. lin.jiang@biology.gatech.edu
[Ti] Título:Alternative community compositional and dynamical states: the dual consequences of assembly history.
[So] Source:J Anim Ecol;80(3):577-85, 2011 May.
[Is] ISSN:1365-2656
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:1. Much work on ecological consequences of community assembly history has focused on the formation of history-induced alternative stable equilibria. We hypothesize that assembly history may affect not only community composition but also population dynamics, with assembled communities differing in species composition potentially residing in different dynamical states. 2. We provided an empirical test of the aforementioned hypothesis using a laboratory microcosm experiment that manipulated both the colonization order of three bacterivorous protist species in the presence of a protist predator and environmental productivity. 3. Both priority effects and random divergence emerged, resulting in two different community compositional states: one characterized by the dominance of one prey species and the other by the extinction of the same prey. While communities in the former state exhibited noncyclic dynamics, the majority of communities in the latter state exhibited cyclic dynamics driven by the interaction between another prey and the predator. 4. Temporal variability of total prey community biovolume consequently differed among communities with different histories. 5. Changing productivity altered priority effects on the structure and dynamics of communities experiencing only certain histories. 6. Our results support the dual (compositional and dynamical) consequences of assembly history and emphasize the importance of incorporating the dynamical view into the field of community assembly.
[Mh] Termos MeSH primário: Biota
[Mh] Termos MeSH secundário: Animais
Cilióforos
Cadeia Alimentar
Densidade Demográfica
Dinâmica Populacional
Tetrahymena pyriformis
Tetrahymenina
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Em] Mês de entrada:1107
[Cu] Atualização por classe:110405
[Lr] Data última revisão:
110405
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:110114
[St] Status:MEDLINE
[do] DOI:10.1111/j.1365-2656.2010.01799.x


  6 / 20 MEDLINE  
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[PMID]:21186493
[Au] Autor:Zhao J; Zhang Q
[Ad] Endereço:Department of Ophthalmology,The First Affiliated Hospital of Liaoning Medical College,Jinzhou 121000,China.
[Ti] Título:[Ultrastructural changes of the trabecular meshwork in glucocorticoid induced glaucoma].
[So] Source:Yan Ke Xue Bao;25(2):119-24, 2010 Nov.
[Is] ISSN:1000-4432
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:PURPOSE: Steroid-induced glaucoma in rabbit ocular was established to observe the ultrastructural changes in trabecular meshwork,and to preliminarily study the mechanisms of increased resistance of aqueous outflow in steroid-induced glaucoma. METHODS: Forty New Zealand white rabbits were randomly divided into four groups, namely,control group (n = 10 rats),drops drug group(n = 10 rats), Injecting drug group(n = 10 rats) and drug combination group (n=10 rats). For three consecutive days before the experiment,using the Schitoz's tonometer to measure intraocular pressure(IOP) at 8am, 10am, and 12am each day,obtained the average,and finally calculated basis IOP of these rabbits. The rabbits eyes in drug combination group of were treated with dexamethasone sodium phosphate solution (0.5%)for 8 weeks,three times a day, and given injection of triamcinolone endure (3 mg) weekly. While the rabbits eyes in the control group were treated with sterile saline in the same period of experiment.After 4 days,IOP of the rabbit was measured by Schitoz's tonometer weekly( the IOP was measured at 8am, 10am, 12am each day for the average value).The rabbit whose IOP was more than 21.97 mmHg and continued for one week is regarded a successful animal model as Corticosteroid. After 8 weeks,the eyes of control group and Corticosteroid rabbits was made for Transmission electron microscopy(TEM) specimen which was used to study the ultrastructural of trabecular meshwork cell. RESULTS: 1.The Rabbit-based IOP was (18.082±2.398) mmHg(n=80),in the drug combination group The IOP of rabbit is up to (24.056±1.245) mmHg after three weeks,which was significantly higher compared with that of(18.254 ± 3.465) mmHg in the control group,the difference had significance(p<0.05),after six weeks, which reached the peak, up to (30.214±0.766) mmHg,in the eighth week, up to (29.144±0.685) mmHg, the IOP of 16 rabbit eyes from 20 rabbit eyes in the exprimental group increased.The positive rate was 80%.2. After treatment with dexamethasone,the abnormality of nucleus of the trabecular meshwork cell was increased, microfilament and microtubules among interstitial cells also increased ,cytoplasmic vacuolation, rough endoplasmic reticulum expansion, as well as an increase in intercellular amorphous material. CONCLUSION: 1.The glucocorticoid-induced high intraocular pressure rabbit model has been successfully established in drug combination group.2.A series of changes in the cytoskeleton observed under the transmission electron microscopy are an important part of glucocorticoid-induced elevation of intraocular pressure.
[Mh] Termos MeSH primário: Glaucoma
Malha Trabecular
[Mh] Termos MeSH secundário: Animais
Glucocorticoides
Pressão Intraocular
Tetrahymenina
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glucocorticoids)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:161020
[Lr] Data última revisão:
161020
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:101228
[St] Status:MEDLINE


  7 / 20 MEDLINE  
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[PMID]:20618643
[Au] Autor:Mercer DR; Washburn JO; Anderson JR
[Ad] Endereço:Department of Environmental Science, Policy, and Management, University of California, Berkeley, CA 94720, USA.
[Ti] Título:Vertical oviposition and Lambornella clarki (Ciliophora: Tetrahymenidae) dispersal by Aedes sierrensis (Diptera: Culicidae) in California.
[So] Source:J Vector Ecol;35(1):20-7, 2010 Jun.
[Is] ISSN:1948-7134
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Vertical stratification of treeholes used by mosquitoes may reflect resource quality or result from interspecific competition. Mosquitoes able to monopolize treeholes with optimal resources may be over-represented in the community. Aedes sierrensis, which is well adapted for the Mediterranean climate of California, has evolved in the absence of interspecific competition, so oviposition should reflect resource quality to a large extent. Artificial oviposition traps mounted at four canopy heights facing north or south on trees in a mixed-oak forest at four elevations of the Pacific Coastal Range were used to assess vertical ovipositional preferences by the western treehole mosquito. Natural dispersal of the ciliated protozoan parasite Lambornella clarki was similarly monitored. Gravid Ae. sierrensis showed no vertical stratification during egg laying in traps. Lambornella clarki were naturally dispersed at relatively low frequency into traps and persisted unless eliminated by larval predation. Aedes sierrensis is not currently constrained into occupying a subset of treeholes. However, invasion of its native range by competitive species may alter oviposition patterns.
[Mh] Termos MeSH primário: Aedes/fisiologia
Aedes/parasitologia
Oviposição/fisiologia
Tetrahymenina/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Animais
California
Feminino
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1012
[Cu] Atualização por classe:151006
[Lr] Data última revisão:
151006
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:100713
[St] Status:MEDLINE
[do] DOI:10.1111/j.1948-7134.2010.00023.x


  8 / 20 MEDLINE  
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[PMID]:20494563
[Au] Autor:Bourland WA; Strüder-Kypke MC
[Ad] Endereço:Department of Biological Sciences, Boise State University MS-1515, 1910 University Avenue, Boise, ID 83725-1515, USA. willbour@me.com
[Ti] Título:Agolohymena aspidocauda nov. gen., nov. spec., a histophagous freshwater tetrahymenid ciliate in the family Deltopylidae (Ciliophora, Hymenostomatia), from Idaho (northwest USA): morphology, ontogenesis and molecular phylogeny.
[So] Source:Eur J Protistol;46(3):221-42, 2010 Aug.
[Is] ISSN:1618-0429
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Morphology, ontogeny and the molecular phylogeny of Agolohymena aspidocauda nov. gen., nov. spec., a new freshwater tetrahymenid ciliate from Idaho, U.S.A, are described. The ontogeny and histophagous mode of nutrition are similar to those of Deltopylum rhabdoidesFauré-Fremiet and Mugard, 1946. The new genus is placed with Deltopylum in the resurrected family Deltopylidae Song and Wilbert, 1989. We emend the diagnostic features of the family to include division by polytomy, right and left somatic kineties extending into the preoral suture, crook-shaped or sigmoid adoral membranelles 1 and 2, markedly reduced adoral membranelle 3 and a tetrahymenid silverline pattern. The main diagnostic features of the new genus are a disc-shaped caudal ciliary array and formation of two types of resting cysts, one smooth and the other bearing tangled tubular or cylindrical lepidosomes. Nuclear small subunit ribosomal RNA gene and mitochondrial cytochrome oxidase subunit 1 gene sequences place the new genus basal within the order Tetrahymenida, well separated from members of the family Tetrahymenidae (Lambornella and Tetrahymena) and also from other tetrahymenids (Colpidium, Dexiostoma, Glaucoma). The genetic divergences between this species and other genera in Tetrahymenida are large enough to suggest placement of the new genus in a separate family. This corroborates the morphological data, since the elaborate caudal ciliary array and the lepidosome-covered resting cyst of this species are not found in other Tetrahymenidae.
[Mh] Termos MeSH primário: Água Doce/parasitologia
Tetrahymenina/classificação
Tetrahymenina/isolamento & purificação
[Mh] Termos MeSH secundário: Análise por Conglomerados
DNA de Protozoário/química
DNA de Protozoário/genética
DNA Ribossômico/química
DNA Ribossômico/genética
Complexo IV da Cadeia de Transporte de Elétrons/genética
Genes de RNAr
Idaho
Proteínas Mitocondriais/genética
Dados de Sequência Molecular
Organelas/ultraestrutura
Filogenia
Proteínas de Protozoários/genética
RNA de Protozoário/genética
RNA Ribossômico 18S/genética
Análise de Sequência de DNA
Tetrahymenina/citologia
Tetrahymenina/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Protozoan); 0 (DNA, Ribosomal); 0 (Mitochondrial Proteins); 0 (Protozoan Proteins); 0 (RNA, Protozoan); 0 (RNA, Ribosomal, 18S); EC 1.9.3.1 (Electron Transport Complex IV)
[Em] Mês de entrada:1010
[Cu] Atualização por classe:100708
[Lr] Data última revisão:
100708
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:100525
[St] Status:MEDLINE
[do] DOI:10.1016/j.ejop.2010.04.003


  9 / 20 MEDLINE  
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[PMID]:19199526
[Au] Autor:Jiang L; Joshi H; Patel SN
[Ad] Endereço:School of Biology, Georgia Institute of Technology, Atlanta, GA 30332, USA. lin.jiang@biology.gatech.edu
[Ti] Título:Predation alters relationships between biodiversity and temporal stability.
[So] Source:Am Nat;173(3):389-99, 2009 Mar.
[Is] ISSN:1537-5323
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Abstract: Ecologists disagree on how diversity affects stability. At the heart of the controversy is the relationship between diversity and population stability, with conflicting findings from both theoretical and empirical studies. To help reconcile these results, we propose that this relationship may depend on trophic complexity, such that positive relations tend to emerge in multitrophic but not single-trophic communities. This hypothesis is based on the premise that stabilizing weak trophic interactions restrain population oscillations associated with strong trophic interactions in diverse multitrophic communities. We tested this hypothesis using simple freshwater bacterivorous protist communities differing in diversity with and without a predatory protist species. Coupling weak and strong trophic interactions reduced population temporal variability of the strong-interacting species, supporting the stabilizing role of weak interactions. In keeping with our hypothesis, predation altered the overall effect of diversity on population temporal stability and, in particular, caused a reversal of the diversity-stability relationship (negative without predators and positive with predators) for the strong-interacting species. A similar role of predation was also observed when examining the relationship between diversity and temporal stability of community biomass. Together, these findings demonstrated strong interactive effects of trophic interactions and diversity on temporal stability of population and community properties.
[Mh] Termos MeSH primário: Biodiversidade
Comportamento Predatório
[Mh] Termos MeSH secundário: Animais
Cilióforos/fisiologia
Densidade Demográfica
Dinâmica Populacional
Tetrahymena/fisiologia
Tetrahymenina/fisiologia
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Em] Mês de entrada:1001
[Cu] Atualização por classe:091023
[Lr] Data última revisão:
091023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:090210
[St] Status:MEDLINE
[do] DOI:10.1086/596540


  10 / 20 MEDLINE  
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[PMID]:17911319
[Au] Autor:Chantangsi C; Lynn DH; Brandl MT; Cole JC; Hetrick N; Ikonomi P
[Ad] Endereço:Department of Integrative Biology, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
[Ti] Título:Barcoding ciliates: a comprehensive study of 75 isolates of the genus Tetrahymena.
[So] Source:Int J Syst Evol Microbiol;57(Pt 10):2412-25, 2007 Oct.
[Is] ISSN:1466-5026
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The mitochondrial cytochrome-c oxidase subunit 1 (cox1) gene has been proposed as a DNA barcode to identify animal species. To test the applicability of the cox1 gene in identifying ciliates, 75 isolates of the genus Tetrahymena and three non-Tetrahymena ciliates that are close relatives of Tetrahymena, Colpidium campylum, Colpidium colpoda and Glaucoma chattoni, were selected. All tetrahymenines of unproblematic species could be identified to the species level using 689 bp of the cox1 sequence, with about 11 % interspecific sequence divergence. Intraspecific isolates of Tetrahymena borealis, Tetrahymena lwoffi, Tetrahymena patula and Tetrahymena thermophila could be identified by their cox1 sequences, showing <0.65 % intraspecific sequence divergence. In addition, isolates of these species were clustered together on a cox1 neighbour-joining (NJ) tree. However, strains identified as Tetrahymena pyriformis and Tetrahymena tropicalis showed high intraspecific sequence divergence values of 5.01 and 9.07 %, respectively, and did not cluster together on a cox1 NJ tree. This may indicate the presence of cryptic species. The mean interspecific sequence divergence of Tetrahymena was about 11 times greater than the mean intraspecific sequence divergence, and this increased to 58 times when all isolates of species with high intraspecific sequence divergence were excluded. This result is similar to DNA barcoding studies on animals, indicating that congeneric sequence divergences are an order of magnitude greater than conspecific sequence divergences. Our analysis also demonstrated low sequence divergences of <1.0 % between some isolates of T. pyriformis and Tetrahymena setosa on the one hand and some isolates of Tetrahymena furgasoni and T. lwoffi on the other, suggesting that the latter species in each pair is a junior synonym of the former. Overall, our study demonstrates the feasibility of using the mitochondrial cox1 gene as a taxonomic marker for 'barcoding' and identifying Tetrahymena species and some other ciliated protists.
[Mh] Termos MeSH primário: Ciclo-Oxigenase 1/genética
Parasitologia/métodos
Tetrahymenina/classificação
Tetrahymenina/genética
[Mh] Termos MeSH secundário: Animais
Análise por Conglomerados
DNA de Protozoário/química
DNA de Protozoário/genética
Dados de Sequência Molecular
Filogenia
Análise de Sequência de DNA
Homologia de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Protozoan); EC 1.14.99.1 (Cyclooxygenase 1)
[Em] Mês de entrada:0801
[Cu] Atualização por classe:071003
[Lr] Data última revisão:
071003
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:071004
[St] Status:MEDLINE



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