Base de dados : MEDLINE
Pesquisa : B01.046.500.700 [Categoria DeCS]
Referências encontradas : 14 [refinar]
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  1 / 14 MEDLINE  
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[PMID]:26253648
[Au] Autor:Geisen S; Bonkowski M; Zhang J; De Jonckheere JF
[Ad] Endereço:Department of Terrestrial Ecology, Institute of Zoology, University of Cologne, 50674 Cologne, Germany. Electronic address: s.geisen@nioo.knaw.nl.
[Ti] Título:Heterogeneity in the genus Allovahlkampfia and the description of the new genus Parafumarolamoeba (Vahlkampfiidae; Heterolobosea).
[So] Source:Eur J Protistol;51(4):335-49, 2015 Aug.
[Is] ISSN:1618-0429
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Heterolobosean amoebae are common and diverse members of soil protist communities. In this study, we isolated seven strains of amoebae from soil samples taken in Tibet (at high altitude), Sardinia and the Netherlands, all resembling to belong to a similar heterolobosean morphospecies. However, sequences of the small subunit (SSU) rDNA and internal transcribed spacers, including the 5.8S rDNA, revealed a high heterogeneity in the genus Allovahlkampfia to which six of the isolates belong. Some unnamed strains, of which the sequences had been published before, are also included within the genus Allovahlkampfia. One Allovahlkampfia isolated in the Netherlands harbors a twin-ribozyme, containing a His-Cys box, similar to the one found in strain BA of Allovahlkampfia. The other SSU rDNA sequence grouped in phylogenetic analyses with sequences obtained in environmental sequencing studies as sister to the genus Fumarolamoeba. This phylogenetic placement was supported by analyses of the 5.8S rDNA leading us to describe it as a new genus Parafumarolamoeba.
[Mh] Termos MeSH primário: Filogenia
Schizopyrenida/classificação
Schizopyrenida/genética
[Mh] Termos MeSH secundário: DNA de Protozoário/genética
DNA Espaçador Ribossômico/genética
Heterogeneidade Genética
Itália
Dados de Sequência Molecular
Países Baixos
RNA Ribossômico 5,8S/genética
Schizopyrenida/citologia
Schizopyrenida/isolamento & purificação
Solo/parasitologia
Especificidade da Espécie
Tibet
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Protozoan); 0 (DNA, Ribosomal Spacer); 0 (RNA, Ribosomal, 5.8S); 0 (Soil)
[Em] Mês de entrada:1511
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150809
[St] Status:MEDLINE


  2 / 14 MEDLINE  
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[PMID]:25253612
[Au] Autor:Tyson JY; Vargas P; Cianciotto NP
[Ad] Endereço:Department of Microbiology and Immunology, Northwestern University Medical School, Chicago, IL 60611, USA.
[Ti] Título:The novel Legionella pneumophila type II secretion substrate NttC contributes to infection of amoebae Hartmannella vermiformis and Willaertia magna.
[So] Source:Microbiology;160(Pt 12):2732-44, 2014 Dec.
[Is] ISSN:1465-2080
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The type II protein secretion (T2S) system of Legionella pneumophila secretes over 25 proteins, including novel proteins that have no similarity to proteins of known function. T2S is also critical for the ability of L. pneumophila to grow within its natural amoebal hosts, including Acanthamoeba castellanii, Hartmannella vermiformis and Naegleria lovaniensis. Thus, T2S has an important role in the natural history of legionnaires' disease. Our previous work demonstrated that the novel T2S substrate NttA promotes intracellular infection of A. castellanii, whereas the secreted RNase SrnA, acyltransferase PlaC, and metalloprotease ProA all promote infection of H. vermiformis and N. lovaniensis. In this study, we determined that another novel T2S substrate that is specific to Legionella, designated NttC, is unique in being required for intracellular infection of H. vermiformis but not for infection of N. lovaniensis or A. castellanii. Expanding our repertoire of amoebal hosts, we determined that Willaertia magna is susceptible to infection by L. pneumophila strains 130b, Philadelphia-1 and Paris. Furthermore, T2S and, more specifically, NttA, NttC and PlaC were required for infection of W. magna. Taken together, these data demonstrate that the T2S system of L. pneumophila is critical for infection of at least four types of aquatic amoebae and that the importance of the individual T2S substrates varies in a host cell-specific fashion. Finally, it is now clear that novel T2S-dependent proteins that are specific to the genus Legionella are particularly important for L. pneumophila infection of key, environmental hosts.
[Mh] Termos MeSH primário: Proteínas de Bactérias/metabolismo
Hartmannella/microbiologia
Legionella pneumophila/fisiologia
Schizopyrenida/microbiologia
Fatores de Virulência/metabolismo
[Mh] Termos MeSH secundário: Proteínas de Bactérias/genética
Sistemas de Secreção Bacterianos
Legionella pneumophila/metabolismo
Fatores de Virulência/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Bacterial Secretion Systems); 0 (Virulence Factors)
[Em] Mês de entrada:1510
[Cu] Atualização por classe:161019
[Lr] Data última revisão:
161019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140926
[St] Status:MEDLINE
[do] DOI:10.1099/mic.0.082750-0


  3 / 14 MEDLINE  
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[PMID]:22929156
[Au] Autor:Arnalich-Montiel F; Lorenzo-Morales J; Irigoyen C; Morcillo-Laiz R; López-Vélez R; Muñoz-Negrete F; Piñero JE; Valladares B
[Ad] Endereço:Cornea Unit, Ophthalmology Department, Ramón y Cajal Hospital, Madrid, Spain. farnalich@gmail.com
[Ti] Título:Co-isolation of Vahlkampfia and acanthamoeba in acanthamoeba-like keratitis in a Spanish population.
[So] Source:Cornea;32(5):608-14, 2013 May.
[Is] ISSN:1536-4798
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PURPOSE: To report the co-isolation incidence of Acanthamoeba and Vahlkampfia in amoebic keratitis from a tertiary care institution in Madrid, Spain. METHODS: In this retrospective case series, 7 eyes of 7 consecutive patients with culture-proven or polymerase chain reaction-positive Acanthamoeba keratitis were seen at a tertiary care institution from January 2010 to April 2011, and their charts were reviewed. RESULTS: Two of 7 patients showed mixed Acanthamoeba and Vahlkampfia keratitis. Good clinical response to the treatment was strongly correlated with early diagnosis, whereas delayed diagnosis resulted in poor response to the treatment in single or mixed infection. CONCLUSIONS: Co-isolation of Vahlkampfia and Acanthamoeba in Acanthamoeba-like keratitis has recently been detected in our population. This finding should raise awareness of the existence of other amoeba different from Acanthamoeba causing keratitis. There are not enough cases yet to determine the impact of mixed amoebic keratitis in the prognosis of this disease.
[Mh] Termos MeSH primário: Ceratite por Acanthamoeba/parasitologia
Acanthamoeba/isolamento & purificação
Schizopyrenida/isolamento & purificação
[Mh] Termos MeSH secundário: Acanthamoeba/genética
Ceratite por Acanthamoeba/diagnóstico
Ceratite por Acanthamoeba/tratamento farmacológico
Adulto
Antifúngicos/uso terapêutico
Antiprotozoários/uso terapêutico
Benzamidinas/uso terapêutico
Biguanidas/uso terapêutico
Linhagem Celular
DNA de Protozoário/análise
Desinfetantes/uso terapêutico
Quimioterapia Combinada
Feminino
Amplificação de Genes
Técnicas de Genotipagem
Seres Humanos
Incidência
Masculino
Meia-Idade
Reação em Cadeia da Polimerase
Pirimidinas/uso terapêutico
RNA Ribossômico 18S/genética
Estudos Retrospectivos
Schizopyrenida/genética
Espanha
Triazóis/uso terapêutico
Voriconazol
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Antiprotozoal Agents); 0 (Benzamidines); 0 (Biguanides); 0 (DNA, Protozoan); 0 (Disinfectants); 0 (Pyrimidines); 0 (RNA, Ribosomal, 18S); 0 (Triazoles); 322U039GMF (polihexanide); G20G12V769 (propamidine); JFU09I87TR (Voriconazole)
[Em] Mês de entrada:1308
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120830
[St] Status:MEDLINE
[do] DOI:10.1097/ICO.0b013e31825697e6


  4 / 14 MEDLINE  
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[PMID]:22067209
[Au] Autor:González-Robles A; Salazar-Villatoro L; González-Lázaro M; Omaña-Molina M; Martínez-Palomo A
[Ad] Endereço:Department of Infectomic and Molecular Pathogenesis, Center for Research and Advanced Studies, Mexico City, Mexico. goroa@cinvestav.mx
[Ti] Título:Vahlkampfia sp: structural observations of cultured trophozoites.
[So] Source:Exp Parasitol;130(1):86-90, 2012 Jan.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Some structural observations on cultured Vahlkampfia sp. trophozoites are reported. Trophozoites are active and pleomorphic, producing large cell protrusions related to locomotion such as lamellipodia, filopodia and endocytic structures formed by hyaline cytoplasm, in which actin provides a framework that allows rapid changes in morphology. As observed by transmission electron microscopy, the cytoplasm is highly granular masking some cell organelles and the major cytoplasmic membrane systems. The structure of cell organelles such as the nucleus, endoplasmic reticulum, and digestive vacuoles is described. A common finding was the presence of 50 nm electron-dense round granules that are not limited by a membrane and that appear scattered in the cytoplasm, and whose function remains unknown. Apparently, the cell reserve material is glycogen, since complete trophozoites were positive to Schiff periodic-acid technique.
[Mh] Termos MeSH primário: Amebíase/parasitologia
Ceratite/parasitologia
Schizopyrenida/ultraestrutura
[Mh] Termos MeSH secundário: Seres Humanos
Microscopia Eletrônica de Varredura
Microscopia Eletrônica de Transmissão
Microscopia de Fluorescência
Microscopia de Contraste de Fase
Polissacarídeos/análise
Schizopyrenida/citologia
Schizopyrenida/crescimento & desenvolvimento
Trofozoítos/citologia
Trofozoítos/crescimento & desenvolvimento
Trofozoítos/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Polysaccharides)
[Em] Mês de entrada:1201
[Cu] Atualização por classe:111205
[Lr] Data última revisão:
111205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:111110
[St] Status:MEDLINE
[do] DOI:10.1016/j.exppara.2011.10.009


  5 / 14 MEDLINE  
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[PMID]:21365453
[Au] Autor:Lasjerdi Z; Niyyati M; Haghighi A; Shahabi S; Biderouni FT; Taghipour N; Eftekhar M; Nazemalhosseini Mojarad E
[Ad] Endereço:Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
[Ti] Título:Potentially pathogenic free-living amoebae isolated from hospital wards with immunodeficient patients in Tehran, Iran.
[So] Source:Parasitol Res;109(3):575-80, 2011 Sep.
[Is] ISSN:1432-1955
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:This study investigated the occurrence of free-living amoebae (FLA) in immunodeficiency wards of hospitals in Tehran, Iran. A total of 70 dust and biofilm samples from wards serving transplant, pediatric (malignancies), HIV, leukemia and oncology patients of five university hospitals were collected and examined for the presence of FLA using culturing and molecular approaches. Based on the morphology of the amoebae in plate cultures, primer sets were applied for molecular identification of Acanthamoeba, vahlkampfiid amoebae and Hartmannella. Out of 70 samples, 37 (52.9%) were positive for FLA. Acanthamoeba belonged to the T4 genotype was the most prevalent isolate. Presence of the T4 genotype on medical instruments, including an oxygen mask in an isolation room of an immunodeficiency pediatric ward, should be of concern for health authorities. Acanthamoeba T5 genotypes, Hartmannella vermiformis, and Vahlkampfia avara were also present. These results highlight a clear need for greater attention to improved disinfection, especially where susceptible patients, such as those who are immune-suppressed, are served. To our knowledge, this is the first report of these FLA in immunodeficiency wards in Iran, and also the first to identify Acanthamoeba T5, Hartmannella, and Vahlkampfia in moist habitats, such as biofilms, in this country.
[Mh] Termos MeSH primário: Acanthamoeba/isolamento & purificação
Microbiologia Ambiental
Hartmannella/isolamento & purificação
Schizopyrenida/isolamento & purificação
[Mh] Termos MeSH secundário: Acanthamoeba/classificação
Acanthamoeba/citologia
Acanthamoeba/genética
Biofilmes
DNA de Protozoário/química
DNA de Protozoário/genética
DNA Espaçador Ribossômico/química
DNA Espaçador Ribossômico/genética
Hartmannella/classificação
Hartmannella/citologia
Hartmannella/genética
Hospitais Universitários
Seres Humanos
Hospedeiro Imunocomprometido
Irã (Geográfico)
Dados de Sequência Molecular
Schizopyrenida/classificação
Schizopyrenida/citologia
Schizopyrenida/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Protozoan); 0 (DNA, Ribosomal Spacer)
[Em] Mês de entrada:1112
[Cu] Atualização por classe:171007
[Lr] Data última revisão:
171007
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:110303
[St] Status:MEDLINE
[do] DOI:10.1007/s00436-011-2288-5


  6 / 14 MEDLINE  
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[PMID]:20494561
[Au] Autor:Murase J; Kawasaki M; De Jonckheere JF
[Ad] Endereço:Graduate School of Bioagricultural Sciences, Nagoya University, 464-8601 Nagoya, Japan. murase@agr.nagoya-u.ac.jp
[Ti] Título:Isolation of a new heterolobosean amoeba from a rice field soil: Vrihiamoeba italica gen. nov., sp. nov.
[So] Source:Eur J Protistol;46(3):164-70, 2010 Aug.
[Is] ISSN:1618-0429
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:A heterolobosean amoeba strain 6_5F was isolated from an Italian rice field soil. Although 18S rRNA gene sequence analysis demonstrated that the new isolate was closely related to Stachyamoeba sp. ATCC 50324, further molecular analysis and morphological observation showed distinct differences amongst the two. The 5.8S rRNA gene was successfully amplified and sequenced for strain 6_5F but not for strain ATCC 50324. Trophozoites of strain ATCC 50324 transform into flagellate forms in the late stage of incubation before encystment, while strain 6_5F do not show flagellate forms under different conditions of the flagellation test. Light and electron microscopic observation showed the structural difference of cysts of strain 6_5F from strain ATCC 50324 and also from the type strain Stachyamoeba lipophora. The results show that the strain 6_5F is distinct from Stachyamoeba spp. and we propose a new genus and species for this isolate, Vrihiamoeba italica gen. nov., sp. nov.
[Mh] Termos MeSH primário: Amoeba/classificação
Amoeba/isolamento & purificação
Microbiologia do Solo
[Mh] Termos MeSH secundário: Amoeba/citologia
Amoeba/genética
Análise por Conglomerados
DNA de Protozoário/química
DNA de Protozoário/genética
DNA Ribossômico/química
DNA Ribossômico/genética
Flagelos
Genes de RNAr
Itália
Microscopia
Microscopia Eletrônica de Transmissão
Dados de Sequência Molecular
Oryza
Filogenia
RNA de Protozoário/genética
RNA Ribossômico 18S/genética
RNA Ribossômico 5,8S/genética
Schizopyrenida/citologia
Schizopyrenida/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Protozoan); 0 (DNA, Ribosomal); 0 (RNA, Protozoan); 0 (RNA, Ribosomal, 18S); 0 (RNA, Ribosomal, 5.8S)
[Em] Mês de entrada:1010
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:100525
[St] Status:MEDLINE
[do] DOI:10.1016/j.ejop.2010.04.002


  7 / 14 MEDLINE  
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[PMID]:19857491
[Au] Autor:Niyyati M; Lorenzo-Morales J; Rezaie S; Rahimi F; Martín-Navarro CM; Mohebali M; Maghsood AH; Farnia S; Valladares B; Rezaeian M
[Ad] Endereço:Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
[Ti] Título:First report of a mixed infection due to Acanthamoeba genotype T3 and Vahlkampfia in a cosmetic soft contact lens wearer in Iran.
[So] Source:Exp Parasitol;126(1):89-90, 2010 Sep.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Acanthamoeba keratitis cases have emerged in the recent years in Iran. In this case, an amoebic keratitis due to a mixed infection with Acanthamoeba and Vahlkampfia species is reported. Corneal scrapes, contact lenses and contact lens cases obtained from the patient were analysed and were positive for cysts of Acanthamoeba and Vahlkampfia genera. Genus-specific PCR was carried out for both genera, confirming the microscopic observations. To our knowledge, this is the first reported case of a possible mixed amoebic infection due to Acanthamoeba and Vahlkampfia and raises awareness within contact lens wearers in Iran.
[Mh] Termos MeSH primário: Ceratite por Acanthamoeba/parasitologia
Acanthamoeba/classificação
Amebíase/parasitologia
Lentes de Contato Hidrofílicas/parasitologia
Schizopyrenida/fisiologia
[Mh] Termos MeSH secundário: Acanthamoeba/genética
Ceratite por Acanthamoeba/diagnóstico
Ceratite por Acanthamoeba/tratamento farmacológico
Adulto
Amebíase/diagnóstico
Amebíase/tratamento farmacológico
Antiprotozoários/uso terapêutico
Benzamidinas/uso terapêutico
Córnea/parasitologia
Feminino
Genótipo
Seres Humanos
Irã (Geográfico)
Dados de Sequência Molecular
Schizopyrenida/classificação
Schizopyrenida/genética
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antiprotozoal Agents); 0 (Benzamidines); 7T9IJ84C42 (propamidine isethionate)
[Em] Mês de entrada:1008
[Cu] Atualização por classe:121115
[Lr] Data última revisão:
121115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:091028
[St] Status:MEDLINE
[do] DOI:10.1016/j.exppara.2009.10.009


  8 / 14 MEDLINE  
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[PMID]:21462554
[Au] Autor:Baumgartner M; Eberhardt S; De Jonckheere JF; Stetter KO
[Ad] Endereço:Lehrstuhl für Mikrobiologie, Universität Regensburg, D-93053 Regensburg, Germany.
[Ti] Título:Tetramitus thermacidophilus n. sp., an amoeboflagellate from acidic hot springs.
[So] Source:J Eukaryot Microbiol;56(2):201-6, 2009 Mar-Apr.
[Is] ISSN:1550-7408
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Tetramitus thermacidophilus n. sp. is a novel thermophilic and acidophilic amoeboflagellate isolated from acidic hot springs in the Caldera Uzon (Kamchatka, Russia) and in Pisciarelli Solfatara (Naples, Italy). We describe it based on physiological, morphological, and sequence data. It was grown in monoxenic culture on the archaeon Acidianus brierleyi as food. Tetramitus thermacidophilus multiplies in a pH range from 1.2 to 5 and in a temperature range from 28 °C to 54 °C. The shortest doubling time was 4.5 h at pH 3 at 45 °C. Its spindle-shaped biflagellated stage was only rarely found in culture. The amoeboid stage shows the typical locomotive form of vahlkampfiid amoebae. Sequence comparisons of the internal transcribed spacer sequences and the small subunit rRNA genes confirm that T. thermacidophilus is a novel species within the genus Tetramitus and that both isolates belong to that species.
[Mh] Termos MeSH primário: Água Doce/parasitologia
Fontes Termais/parasitologia
Schizopyrenida/classificação
Schizopyrenida/isolamento & purificação
[Mh] Termos MeSH secundário: Acidianus
Sequência de Bases
Dieta
Ecossistema
Temperatura Alta
Concentração de Íons de Hidrogênio
Itália
Dados de Sequência Molecular
Filogenia
RNA de Protozoário/genética
RNA Ribossômico/genética
RNA Ribossômico 16S/genética
Federação Russa
Schizopyrenida/citologia
Schizopyrenida/genética
Alinhamento de Sequência
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Protozoan); 0 (RNA, Ribosomal); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1106
[Cu] Atualização por classe:110404
[Lr] Data última revisão:
110404
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:110406
[St] Status:MEDLINE


  9 / 14 MEDLINE  
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[PMID]:19602081
[Au] Autor:Visvesvara GS; Sriram R; Qvarnstrom Y; Bandyopadhyay K; Da Silva AJ; Pieniazek NJ; Cabral GA
[Ad] Endereço:Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30341-3724, USA. gsv1@cdc.gov
[Ti] Título:Paravahlkampfia francinae n. sp. masquerading as an agent of primary amoebic meningoencephalitis.
[So] Source:J Eukaryot Microbiol;56(4):357-66, 2009 Jul-Aug.
[Is] ISSN:1550-7408
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Paravahlkampfia francinae n. sp., a new species of the free-living amoeba genus Paravahlkampfia, designated as CDC:V595, was isolated from the cerebrospinal fluid of a patient with headache, sore throat, and vomiting, typical symptoms of primary amoebic meningoencephalitis (PAM) caused by Naegleria fowleri. The isolate grew at 33 degrees C, 37 degrees C, 40 degrees C, and 42 degrees C and destroyed mammalian cell cultures. However, it did not kill young mice upon intranasal inoculation. P. francinae does not produce flagellates and does not grow on agar plates coated with Gram-negative bacteria such as Escherichia coli, the usual food source of Paravahlkampfia ustiana, the type species of the genus. The trophozoite at light microscopy exhibited eruptive locomotion and possessed a single vesicular nucleus. Ultrastructurally, the trophozoites had numerous mitochondria with discoidal cristae but did not have a Golgi apparatus. The trophozoites differentiated into cysts after consuming most of the monolayer. The cyst had an inner well-differentiated endocyst and an outer thin, wrinkled, and wavy ectocyst with no pores. During excystation trophozoites ruptured the cyst wall and emerged from the cysts. A unique feature seen in the cysts was the presence of bacterial endosymbionts, both in the endoplasm and within the cyst wall. Full-length sequencing analysis of the 18S and 5.8S RNA genes of P. francinae showed that they were distinct from those of other Paravahlkampfia species. The patient recovered within a few days indicating that some of the previously reported cases of PAM that survived may have been due to P. francinae.
[Mh] Termos MeSH primário: Infecções Protozoárias do Sistema Nervoso Central/parasitologia
Infecções por Protozoários/parasitologia
Schizopyrenida/fisiologia
Schizopyrenida/patogenicidade
Schizopyrenida/ultraestrutura
[Mh] Termos MeSH secundário: Adolescente
Anfotericina B/administração & dosagem
Animais
Antiprotozoários/administração & dosagem
DNA de Protozoário/análise
DNA de Protozoário/genética
Genes de RNAr
Seres Humanos
Masculino
Camundongos
Microscopia Eletrônica
Dados de Sequência Molecular
Filogenia
Schizopyrenida/efeitos dos fármacos
Especificidade da Espécie
Virulência
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Antiprotozoal Agents); 0 (DNA, Protozoan); 7XU7A7DROE (Amphotericin B)
[Em] Mês de entrada:0909
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:090716
[St] Status:MEDLINE
[do] DOI:10.1111/j.1550-7408.2009.00410.x


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[PMID]:15068271
[Au] Autor:Brugerolle G; Simpson AG
[Ad] Endereço:Biologie des Protistes, UMR CNRS 6023 and Université Blaise Pascal de Clermont-Ferrand, 63177 Aubière, France. Guy.Brugerolle@lbp.univ-bpclermont.fr
[Ti] Título:The flagellar apparatus of heteroloboseans.
[So] Source:J Eukaryot Microbiol;51(1):96-107, 2004 Jan-Feb.
[Is] ISSN:1066-5234
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The flagellar apparatus of four heterolobosean species Percolomonas descissus, Percolomonas sulcatus, Tetramitus rostratus, and Naegleria gruberi were examined. P. descissus lives in oxygen-poor water. It is a quadriflagellated cell with a ventral groove. The two pairs of basal bodies are connected to an apical structure from which the peripheral dorso-lateral microtubules and a short striated rhizoplast originate. There is one major microtubular root, R1, which originates from the posterior basal body pair and splits into left and right portions that support the sides of the ventral groove. The anterior pair of basal bodies is associated with a root of four to five microtubules that runs to the left of the groove. This organisation is similar to that previously reported for Psalteriomonas, Lyromonas, and Percolomonas cosmopolitus. Percolomonas sulcatus has two parallel pairs of basal bodies, each of which is associated with a well-developed R1 root. These roots divide to give two distinct left portions and one merged right portion that support the margins of the slit-like ventral groove. Tetramitus rostratus has two pairs of basal bodies, several rhizoplast fibres, and two R1 roots. Each R1 root supports one wall of the ventral groove. Naegleria gruberi may have two pairs of basal bodies, each associated with a microtubular root and one long rhizoplast fibre. From available data, a 'double bikont'-like organisation of the heterolobosean flagellar apparatus is inferred, where both of the eldest basal bodies have largely 'mature' complements of microtubular roots. The cytoskeletal organisation of heteroloboseans is compared to those of (other) excavates. Our structural data and existing molecular phylogenies weaken the case that Percolomonas, Psalteriomonas, and Lyromonas are phylogenetically separable from other heteroloboseans, undermining many of the highest-level taxa proposed for these organisms, including Percolozoa, Striatorhiza, Percolomonada, Percolomonadea, and Lyromonadea.
[Mh] Termos MeSH primário: Flagelos/fisiologia
Naegleria/fisiologia
Schizopyrenida/fisiologia
[Mh] Termos MeSH secundário: Animais
Austrália
Meio Ambiente
Flagelos/ultraestrutura
Microscopia Eletrônica
Naegleria/ultraestrutura
Schizopyrenida/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:0407
[Cu] Atualização por classe:040407
[Lr] Data última revisão:
040407
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:040408
[St] Status:MEDLINE



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