Base de dados : MEDLINE
Pesquisa : B01.046.550 [Categoria DeCS]
Referências encontradas : 8 [refinar]
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  1 / 8 MEDLINE  
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[PMID]:26411260
[Au] Autor:Swaminathan K; Stumpf M; Müller R; Horn AC; Schmidbauer J; Eichinger L; Müller-Taubenberger A; Faix J; Noegel AA
[Ad] Endereço:Center for Biochemistry, Medical Faculty, Center for Molecular Medicine Cologne (CMMC) and Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, 50931 Köln, Germany.
[Ti] Título:Coronin7 regulates WASP and SCAR through CRIB mediated interaction with Rac proteins.
[So] Source:Sci Rep;5:14437, 2015 Sep 28.
[Is] ISSN:2045-2322
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Coronin7 (CRN7) stabilizes F-actin and is a regulator of processes associated with the actin cytoskeleton. Its loss leads to defects in phagocytosis, motility and development. It harbors a CRIB (Cdc42- and Rac-interactive binding) domain in each of its WD repeat domains which bind to Rac GTPases preferably in their GDP-loaded forms. Expression of wild type CRN7 in CRN7 deficient cells rescued these defects, whereas proteins with mutations in the CRIB motifs which were associated with altered Rac binding were effective to varying degrees. The presence of one functional CRIB was sufficient to reestablish phagocytosis, cell motility and development. Furthermore, by molecular modeling and mutational analysis we identified the contact regions between CRN7 and the GTPases. We also identified WASP, SCAR and PAKa as downstream effectors in phagocytosis, development and cell surface adhesion, respectively, since ectopic expression rescued these functions.
[Mh] Termos MeSH primário: Proteínas dos Microfilamentos/metabolismo
Domínios e Motivos de Interação entre Proteínas
Proteínas de Protozoários/metabolismo
Proteína da Síndrome de Wiskott-Aldrich/metabolismo
Proteínas rac de Ligação ao GTP/metabolismo
[Mh] Termos MeSH secundário: Motivos de Aminoácidos
Sequência de Aminoácidos
Adesão Celular
Proteínas Quinases Dependentes de AMP Cíclico/metabolismo
Proteínas dos Microfilamentos/química
Modelos Moleculares
Dados de Sequência Molecular
Mutação
Micetozoários
Fagocitose
Ligação Proteica
Conformação Proteica
Domínios e Motivos de Interação entre Proteínas/genética
Alinhamento de Sequência
Relação Estrutura-Atividade
Proteínas rac de Ligação ao GTP/química
Proteínas rac de Ligação ao GTP/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Microfilament Proteins); 0 (Protozoan Proteins); 0 (SCAR protein, Dictyostelium); 0 (Wiskott-Aldrich Syndrome Protein); 145420-64-0 (coronin proteins); EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases); EC 3.6.5.2 (rac GTP-Binding Proteins)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150929
[St] Status:MEDLINE
[do] DOI:10.1038/srep14437


  2 / 8 MEDLINE  
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[PMID]:24604300
[Au] Autor:Song X; Zhong H; Wang Q; Li Y
[Ad] Endereço:Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun, 130118, People's Republic of China, sxx8866@163.com.
[Ti] Título:Morphogenesis and alpha-tubulin gene of plasmodium in Didymium megalosporum.
[So] Source:Arch Microbiol;196(5):369-74, 2014 May.
[Is] ISSN:1432-072X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The morphogenesis of plasmodium in Didymium megalosporum was observed for the first time by hanging drop culture and 3 % oat-agar culture under controlled conditions. The development of plasmodium was characteristic formation process of phaneroplasmodium. The mature plasmodium was white yellow or yellow green in color, and had an extending fan-like sheet at the front, followed by a network of veins. It could be easy to fuse into a bigger plasmodium during the formation and die at high temperature or starvation. In view of the important role of alpha-tubulin during the morphogenesis of plasmodium, we sequenced partial sequence of alpha-tubulin gene, a total length of 1,159 bp, in this plasmodium. It had an intron area from 177 to 235 bp, and the exon area had a similarity of 91 % relative to altA locus of alpha-tubulin gene in Physarum polycephalum, the translated amino acid sequence was identical (100 % match) between the two.
[Mh] Termos MeSH primário: Micetozoários/crescimento & desenvolvimento
Micetozoários/genética
Tubulina (Proteína)/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Sequência de Bases
Dados de Sequência Molecular
Morfogênese
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Tubulin)
[Em] Mês de entrada:1405
[Cu] Atualização por classe:140416
[Lr] Data última revisão:
140416
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140308
[St] Status:MEDLINE
[do] DOI:10.1007/s00203-014-0971-5


  3 / 8 MEDLINE  
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[PMID]:25509133
[Au] Autor:Chistiakova LV; Miteva OA; Frolov AO; Skarlato SO
[Ti] Título:[Comparative morphology of the subphilum Conosa Cavalier-Smith 1998].
[So] Source:Tsitologiia;55(11):778-87, 2013.
[Is] ISSN:0041-3771
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:Comparative analysis of archamoebae and slime molds morphology revealed that this organisms have a marked similarity in organization of locomotive forms, structure of glycocalix and also in organization of nuclear and flagellar apparatus. A possible scheme of formation the modern diversity of Conosa group was proposed.
[Mh] Termos MeSH primário: Archamoebae/ultraestrutura
Micetozoários/ultraestrutura
Filogenia
[Mh] Termos MeSH secundário: Archamoebae/classificação
Archamoebae/fisiologia
Evolução Biológica
Núcleo Celular/ultraestrutura
Flagelos/ultraestrutura
Glicocálix/ultraestrutura
Micetozoários/classificação
Micetozoários/fisiologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; ENGLISH ABSTRACT; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1412
[Cu] Atualização por classe:141211
[Lr] Data última revisão:
141211
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141217
[St] Status:MEDLINE


  4 / 8 MEDLINE  
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[PMID]:23842763
[Au] Autor:Carmarán CC; Rosenfeldt S; Skigin D; Inchaussandague M; Keller HW
[Ad] Endereço:Departamento de Biodiversidad y Biología Experimental, FCEN, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón II, C1428EHA, Buenos Aires, Argentina, carmaran@bg.fcen.uba.ar.
[Ti] Título:Autofluorescence and ultrastructure in the Myxomycete Diachea leucopodia (Physarales).
[So] Source:Curr Microbiol;67(6):674-8, 2013 Dec.
[Is] ISSN:1432-0991
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Autofluorescence is reported for the first time in Myxomycete fruiting bodies. Ultrastructure of stalked sporangia of Diachea leucopodia (Didymiaceae, Physarales) was studied using scanning and transmission electron microscopy, energy-dispersive X-ray microanalysis, and fluorescence microscopy. External and internal properties of the peridium that surround the spores and capillitium exhibit autofluorescence. The stalk is composed of calcareous granules and energy-dispersive X-ray microanalysis demonstrates that the elemental composition of the peridium, capillitium, and stalk has varying concentrations of calcium.
[Mh] Termos MeSH primário: Fenômenos Químicos
Micetozoários/química
Micetozoários/ultraestrutura
Esporângios/química
Esporângios/ultraestrutura
[Mh] Termos MeSH secundário: Fluorescência
Microscopia Eletrônica de Varredura
Microscopia Eletrônica de Transmissão
Microscopia de Fluorescência
Espectrometria por Raios X
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1405
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130712
[St] Status:MEDLINE
[do] DOI:10.1007/s00284-013-0418-2


  5 / 8 MEDLINE  
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[PMID]:20159952
[Au] Autor:Traphagen SJ; Dimarco MJ; Silliker ME
[Ad] Endereço:The English High School, Boston Public Schools, Boston, Massachusetts 02130, USA.
[Ti] Título:RNA editing of 10 Didymium iridis mitochondrial genes and comparison with the homologous genes in Physarum polycephalum.
[So] Source:RNA;16(4):828-38, 2010 Apr.
[Is] ISSN:1469-9001
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Regions of the Didymium iridis mitochondrial genome were identified with similarity to typical mitochondrial genes; however, these regions contained numerous stop codons. We used RT-PCR and DNA sequencing to determine whether, through RNA editing, these regions were transcribed into mRNAs that could encode functional proteins. Ten putative gene regions were examined: atp1, atp6, atp8, atp9, cox1, cox2, cytb, nad4L, nad6, and nad7. The cDNA sequences of each gene could encode a functional mitochondrial protein that was highly conserved compared with homologous genes. The type of editing events and editing sequence features were very similar to those observed in the homologous genes of Physarum polycephalum, though the actual editing locations showed a variable degree of conservation. Edited sites were compared with encoded sites in D. iridis and P. polycephalum for all 10 genes. Edited sequence for a portion of the cox1 gene was available for six myxomycetes, which, when compared, showed a high degree of conservation at the protein level. Different types of editing events showed varying degrees of site conservation with C-to-U base changes being the least conserved. Several aspects of single C insertion editing events led to the preferential creation of hydrophobic amino acid codons that may help to minimize adverse effects on the resulting protein structure.
[Mh] Termos MeSH primário: Genes Mitocondriais
Genes de Protozoários
Micetozoários/genética
Edição de RNA/genética
RNA de Protozoário/genética
[Mh] Termos MeSH secundário: Aminoácidos/química
Aminoácidos/metabolismo
Códon
Sequência Conservada
Physarum polycephalum/genética
Proteínas de Protozoários
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Amino Acids); 0 (Codon); 0 (Protozoan Proteins); 0 (RNA, Protozoan)
[Em] Mês de entrada:1005
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:100218
[St] Status:MEDLINE
[do] DOI:10.1261/rna.1989310


  6 / 8 MEDLINE  
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[PMID]:20113379
[Au] Autor:Fiore-Donno AM; Kamono A; Chao EE; Fukui M; Cavalier-Smith T
[Ad] Endereço:Zoology Department, University of Oxford, South Parks Road, OX13PS Oxford, United Kingdom. afiore-donno6@infomaniak.ch
[Ti] Título:Invalidation of Hyperamoeba by transferring its species to other genera of Myxogastria.
[So] Source:J Eukaryot Microbiol;57(2):189-96, 2010 Mar-Apr.
[Is] ISSN:1550-7408
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The genus Hyperamoeba Alexeieff, 1923 was established to accommodate an aerobic amoeba exhibiting three life stages-amoeba, flagellate, and cyst. As more species/strains were isolated, it became increasingly evident from small subunit (SSU) gene phylogenies and ultrastructure that Hyperamoeba is polyphyletic and its species occupy different positions within the class Myxogastria. To pinpoint Hyperamoeba strains within other myxogastrid genera we aligned numerous myxogastrid sequences: whole small subunit ribosomal (SSU or 18S rRNA) gene for 50 dark-spored (i.e. Stemonitida and Physarida) Myxogastria (including a new "Hyperamoeba"/Didymium sequence) and a approximately 400-bp SSU fragment for 147 isolates assigned to 10 genera of the order Physarida. Phylogenetic analyses show unambiguously that the type species Hyperamoeba flagellata is a Physarum (Physarum flagellatum comb. nov.) as it nests among other Physarum species as robust sister to Physarum didermoides. Our trees also allow the following allocations: five Hyperamoeba strains to the genus Stemonitis; Hyperamoeba dachnaya, Pseudodidymium cryptomastigophorum, and three other Hyperamoeba strains to the genus Didymium; and two further Hyperamoeba strains to the family Physaridae. We therefore abandon the polyphyletic and redundant genus Hyperamoeba. We discuss the implications for the ecology and evolution of Myxogastria, whose amoeboflagellates are more widespread than previous inventories supposed, being now found in freshwater and even marine environments.
[Mh] Termos MeSH primário: Micetozoários/classificação
Micetozoários/genética
[Mh] Termos MeSH secundário: Análise por Conglomerados
DNA de Protozoário/química
DNA de Protozoário/genética
DNA Ribossômico/química
DNA Ribossômico/genética
Genes de RNAr
Filogenia
RNA de Protozoário/genética
RNA Ribossômico 18S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Protozoan); 0 (DNA, Ribosomal); 0 (RNA, Protozoan); 0 (RNA, Ribosomal, 18S)
[Em] Mês de entrada:1008
[Cu] Atualização por classe:101110
[Lr] Data última revisão:
101110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:100202
[St] Status:MEDLINE
[do] DOI:10.1111/j.1550-7408.2009.00466.x


  7 / 8 MEDLINE  
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[PMID]:19656720
[Au] Autor:Fiore-Donno AM; Nikolaev SI; Nelson M; Pawlowski J; Cavalier-Smith T; Baldauf SL
[Ad] Endereço:University of Oxford, Department of Zoology, South Parks Road, Oxford OX1 3PS, UK. afiore-donno6@infomaniak.ch
[Ti] Título:Deep phylogeny and evolution of slime moulds (mycetozoa).
[So] Source:Protist;161(1):55-70, 2010 Jan.
[Is] ISSN:1618-0941
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Mycetozoa, characterized by spore-bearing fruiting bodies, are the most diverse Amoebozoa. They traditionally comprise three taxa: Myxogastria, Dictyostelia and Protostelia. Myxogastria and Dictyostelia typically have multispored fruiting bodies, but controversy exists whether they are related or arose independently from different unicellular ancestors. Protostelid slime moulds, with single-spored fruiting bodies, are possible evolutionary intermediates between them and typical amoebae, but have received almost no molecular study. Protostelid morphology is so varied that they might not be monophyletic. We therefore provide 38 new 18S rRNA and/or EF-1alpha gene sequences from Mycetozoa and related species, including four protostelids and the enigmatic Ceratiomyxa fruticulosa. Phylogenetic analyses support the monophyly of Dictyostelia, Myxogastria, and Ceratiomyxa (here collectively called "macromycetozoa") and show that protostelids are Amoebozoa, mostly related to non-fruiting amoebae of the class Variosea, but may not be monophyletic; some phylogenetic relationships remain poorly resolved. Ceratiomyxa fruticulosa, originally regarded as a myxogastrid, but in recent decades included in Protostelia, is a deeply diverging sister to Myxogastria. The protostelids studied here plus varipodid amoebae and the flagellates Phalansterium and Multicilia together probably form the outgroup to macromycetozoa plus Archamoebae. Thus protostelids and Variosea are especially significant for understanding the evolutionary transition from solitary amoebae to macromycetozoa.
[Mh] Termos MeSH primário: Evolução Molecular
Micetozoários/classificação
Micetozoários/genética
Filogenia
[Mh] Termos MeSH secundário: Animais
Análise por Conglomerados
DNA de Protozoário/química
DNA de Protozoário/genética
DNA Ribossômico/química
DNA Ribossômico/genética
Genes de RNAr
Dados de Sequência Molecular
Micetozoários/citologia
RNA de Protozoário/genética
RNA Ribossômico 18S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Protozoan); 0 (DNA, Ribosomal); 0 (RNA, Protozoan); 0 (RNA, Ribosomal, 18S)
[Em] Mês de entrada:1004
[Cu] Atualização por classe:100204
[Lr] Data última revisão:
100204
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:090807
[St] Status:MEDLINE
[do] DOI:10.1016/j.protis.2009.05.002


  8 / 8 MEDLINE  
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[PMID]:19707546
[Au] Autor:Shadwick LL; Spiegel FW; Shadwick JD; Brown MW; Silberman JD
[Ad] Endereço:Department of Biological Sciences, University of Arkansas, Fayetteville, Arkansas, USA. lora.lindley@gmail.com
[Ti] Título:Eumycetozoa = Amoebozoa?: SSUrDNA phylogeny of protosteloid slime molds and its significance for the amoebozoan supergroup.
[So] Source:PLoS One;4(8):e6754, 2009 Aug 25.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Amoebae that make fruiting bodies consisting of a stalk and spores and classified as closely related to the myxogastrids have classically been placed in the taxon Eumycetozoa. Traditionally, there are three groups comprising Eumycetozoa: myxogastrids, dictyostelids, and the so-called protostelids. Dictyostelids and myxogastrids both make multicellular fruiting bodies that may contain hundreds of spores. Protostelids are those amoebae that make simple fruiting bodies consisting of a stalk and one or a few spores. Protostelid-like organisms have been suggested as the progenitors of the myxogastrids and dictyostelids, and they have been used to formulate hypotheses on the evolution of fruiting within the group. Molecular phylogenies have been published for both myxogastrids and dictyostelids, but little molecular phylogenetic work has been done on the protostelids. Here we provide phylogenetic trees based on the small subunit ribosomal RNA gene (SSU) that include 21 protostelids along with publicly available sequences from a wide variety of amoebae and other eukaryotes. SSU trees recover seven well supported clades that contain protostelids but do not appear to be specifically related to one another and are often interspersed among established groups of amoebae that have never been reported to fruit. In fact, we show that at least two taxa unambiguously belong to amoebozoan lineages where fruiting has never been reported. These analyses indicate that we can reject a monophyletic Eumycetozoa, s.l. For this reason, we will hereafter refer to those slime molds with simple fruiting as protosteloid amoebae and/or protosteloid slime molds, not as protostelids. These results add to our understanding of amoebozoan biodiversity, and demonstrate that the paradigms for understanding both nonfruiting and sporulating amoebae must be integrated. Finally, we suggest strategies for future research on protosteloid amoebae and nonfruiting amoebae, and discuss the impact of this work for taxonomists and phylogenomicists.
[Mh] Termos MeSH primário: DNA Ribossômico/genética
Micetozoários/genética
Filogenia
[Mh] Termos MeSH secundário: Animais
Funções Verossimilhança
Micetozoários/classificação
Reação em Cadeia da Polimerase
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (DNA, Ribosomal)
[Em] Mês de entrada:1001
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:090827
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0006754



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