Base de dados : MEDLINE
Pesquisa : B01.046.550.200.300 [Categoria DeCS]
Referências encontradas : 6906 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 691 ir para página                         

  1 / 6906 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29286727
[Au] Autor:Segota I; Franck C
[Ad] Endereço:Laboratory of Atomic and Solid State Physics, Cornell University, Ithaca 14853, USA.
[Ti] Título:Extracellular Processing of Molecular Gradients by Eukaryotic Cells Can Improve Gradient Detection Accuracy.
[So] Source:Phys Rev Lett;119(24):248101, 2017 Dec 15.
[Is] ISSN:1079-7114
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Eukaryotic cells sense molecular gradients by measuring spatial concentration variation through the difference in the number of occupied receptors to which molecules can bind. They also secrete enzymes that degrade these molecules, and it is presently not well understood how this affects the local gradient perceived by cells. Numerical and analytical results show that these enzymes can substantially increase the signal-to-noise ratio of the receptor difference and allow cells to respond to a much broader range of molecular concentrations and gradients than they would without these enzymes.
[Mh] Termos MeSH primário: AMP Cíclico/metabolismo
Células Eucarióticas/metabolismo
Modelos Biológicos
Diester Fosfórico Hidrolases/metabolismo
[Mh] Termos MeSH secundário: Ácido Aspártico Endopeptidases/metabolismo
Quimiotaxia
Dictyostelium/enzimologia
Dictyostelium/metabolismo
Difusão
Células Eucarióticas/citologia
Células Eucarióticas/enzimologia
Saccharomyces cerevisiae/enzimologia
Saccharomyces cerevisiae/metabolismo
Proteínas de Saccharomyces cerevisiae/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Saccharomyces cerevisiae Proteins); E0399OZS9N (Cyclic AMP); EC 3.1.4.- (Phosphoric Diester Hydrolases); EC 3.4.23.- (Aspartic Acid Endopeptidases); EC 3.4.23.- (BAR1 protein, S cerevisiae)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180109
[Lr] Data última revisão:
180109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171230
[St] Status:MEDLINE
[do] DOI:10.1103/PhysRevLett.119.248101


  2 / 6906 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28760625
[Au] Autor:Lee HM; Seo JH; Kwak MK; Kang SO
[Ad] Endereço:Laboratory of Biophysics, School of Biological Sciences, and Institute of Microbiology, Seoul National University, Seoul 151-742, Republic of Korea.
[Ti] Título:Methylglyoxal upregulates Dictyostelium discoideum slug migration by triggering glutathione reductase and methylglyoxal reductase activity.
[So] Source:Int J Biochem Cell Biol;90:81-92, 2017 Sep.
[Is] ISSN:1878-5875
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Glutathione (GSH)-deprived Dictyostelium discoideum accumulates methylglyoxal (MG) and reactive oxygen species (ROS) during vegetative growth. However, the reciprocal effects of the production and regulation of these metabolites on differentiation and cell motility are unclear. Based on the inhibitory effects of γ-glutamylcysteine synthetase (gcsA) disruption and GSH reductase (gsr) overexpression on aggregation and culmination, respectively, we overexpressed GSH-related genes encoding superoxide dismutase (Sod2), catalase (CatA), and Gcs, in D. discoideum. Wild-type KAx3 and gcsA-overexpressing (gcsA ) slugs maintained GSH levels at levels of approximately 2.1-fold less than the reference GSH synthetase-overexpressing mutant; their GSH levels did not correlate with slug migration ability. Through prolonged KAx3 migration by treatment with MG and H O , we found that MG increased after the mound stage in this strain, with a 2.6-fold increase compared to early developmental stages; in contrast, ROS were maintained at high levels throughout development. While the migration-defective sod2- and catA-overexpressing mutant slugs (sod2 and catA ) decreased ROS levels by 50% and 53%, respectively, these slugs showed moderately decreased MG levels (36.2±5.8 and 40.7±1.6nmolg cells wet weight, P<0.05) compared to the parental strain (54.2±3.5nmolg ). Importantly, defects in the migration of gcsA slugs decreased MG considerably (13.8±4.2nmolg , P<0.01) along with a slight decrease in ROS. In contrast to the increase observed in migrating sod2 and catA slugs by treatment with MG and H O , the migration of gcsA slugs appeared unaffected. This behavior was caused by MG-triggered Gsr and NADPH-linked aldolase reductase activity, suggesting that GSH biosynthesis in gcsA slugs is specifically used for MG-scavenging activity. This is the first report showing that MG upregulates slug migration via MG-scavenging-mediated differentiation.
[Mh] Termos MeSH primário: Oxirredutases do Álcool/metabolismo
Dictyostelium/enzimologia
Dictyostelium/fisiologia
Glutationa Redutase/metabolismo
Aldeído Pirúvico/farmacologia
Regulação para Cima/efeitos dos fármacos
[Mh] Termos MeSH secundário: Oxirredutases do Álcool/genética
Dictyostelium/efeitos dos fármacos
Dictyostelium/genética
Glutationa/metabolismo
Glutationa Redutase/genética
Peróxido de Hidrogênio/farmacologia
Mutação
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Espécies Reativas de Oxigênio/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Messenger); 0 (Reactive Oxygen Species); 722KLD7415 (Pyruvaldehyde); BBX060AN9V (Hydrogen Peroxide); EC 1.1.- (Alcohol Oxidoreductases); EC 1.1.1.78 (D-lactaldehyde dehydrogenase); EC 1.8.1.7 (Glutathione Reductase); GAN16C9B8O (Glutathione)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170802
[St] Status:MEDLINE


  3 / 6906 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28727774
[Au] Autor:Ouertatani-Sakouhi H; Kicka S; Chiriano G; Harrison CF; Hilbi H; Scapozza L; Soldati T; Cosson P
[Ad] Endereço:Faculty of Medicine, Department of Cell Physiology and Metabolism, University of Geneva, Geneva, Switzerland.
[Ti] Título:Inhibitors of Mycobacterium marinum virulence identified in a Dictyostelium discoideum host model.
[So] Source:PLoS One;12(7):e0181121, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Tuberculosis remains one of the major threats to public health worldwide. Given the prevalence of multi drug resistance (MDR) in Mycobacterium tuberculosis strains, there is a strong need to develop new anti-mycobacterial drugs with modes of action distinct from classical antibiotics. Inhibitors of mycobacterial virulence might target new molecular processes and may represent a potential new therapeutic alternative. In this study, we used a Dictyostelium discoideum host model to assess virulence of Mycobacterium marinum and to identify compounds inhibiting mycobacterial virulence. Among 9995 chemical compounds, we selected 12 inhibitors of mycobacterial virulence that do not inhibit mycobacterial growth in synthetic medium. Further analyses revealed that 8 of them perturbed functions requiring an intact mycobacterial cell wall such as sliding motility, bacterial aggregation or cell wall permeability. Chemical analogs of two compounds were analyzed. Chemical modifications altered concomitantly their effect on sliding motility and on mycobacterial virulence, suggesting that the alteration of the mycobacterial cell wall caused the loss of virulence. We characterized further one of the selected compounds and found that it inhibited the ability of mycobacteria to replicate in infected cells. Together these results identify new antimycobacterial compounds that represent new tools to unravel the molecular mechanisms controlling mycobacterial pathogenicity. The isolation of compounds with anti-virulence activity is the first step towards developing new antibacterial treatments.
[Mh] Termos MeSH primário: Dictyostelium/microbiologia
Mycobacterium marinum/efeitos dos fármacos
Virulência/efeitos dos fármacos
[Mh] Termos MeSH secundário: Avaliação Pré-Clínica de Medicamentos/métodos
Mycobacterium marinum/patogenicidade
Mycobacterium marinum/fisiologia
Mycobacterium marinum/ultraestrutura
Bibliotecas de Moléculas Pequenas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Small Molecule Libraries)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170721
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0181121


  4 / 6906 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28711497
[Au] Autor:Ilacqua AN; Shettler JA; Wernke KM; Skalla JK; McQuade KJ
[Ad] Endereço:Department of Biological Sciences, Colorado Mesa University, Grand Junction, CO 81501, USA.
[Ti] Título:Theaflavins from black tea affect growth, development, and motility in Dictyostelium discoideum.
[So] Source:Biochem Biophys Res Commun;491(2):449-454, 2017 Sep 16.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Theaflavins, flavonoids found in black tea, exhibit a variety of health-promoting activities, but the mechanisms by which they act are not clear. Here, we assess the effects of black tea extract and isolated theaflavins on Dictyostelium discoideum, a model organism exhibiting an unusual life cycle relying on conserved pathways involved in human disease. Dictyostelium has been used to characterize the activities of numerous bioactive small molecules, including catechins, from which theaflavins are produced during the preparation of black tea. We show that theaflavins block growth, development, and motility in Dictyostelium, results that suggest catechins and theaflavins exert similar activities in this organism.
[Mh] Termos MeSH primário: Biflavonoides/farmacologia
Camellia sinensis/química
Catequina/farmacologia
Catecóis/farmacologia
Dictyostelium/efeitos dos fármacos
[Mh] Termos MeSH secundário: Cultura Axênica
Biflavonoides/química
Biflavonoides/isolamento & purificação
Catequina/química
Catequina/isolamento & purificação
Catecóis/química
Catecóis/isolamento & purificação
Movimento Celular/efeitos dos fármacos
Movimento Celular/fisiologia
Dictyostelium/crescimento & desenvolvimento
Extratos Vegetais/química
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biflavonoids); 0 (Catechols); 0 (Plant Extracts); 1IA46M0D13 (theaflavin); 8R1V1STN48 (Catechin)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170829
[Lr] Data última revisão:
170829
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170717
[St] Status:MEDLINE


  5 / 6906 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28665127
[Au] Autor:Mik V; Micková Z; Dolezal K; Frébort I; Pospísil T
[Ad] Endereço:Department of Chemical Biology and Genetics and ‡Department of Molecular Biology, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University , Olomouc 771 47, Czech Republic.
[Ti] Título:Activity of (+)-Discadenine as a Plant Cytokinin.
[So] Source:J Nat Prod;80(7):2136-2140, 2017 Jul 28.
[Is] ISSN:1520-6025
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Discadenine (1), a self-spore germination inhibitor from the cellular slim mold Dictyostelium discoideum, is structurally related to the plant hormone cytokinin. This compound was synthesized from l-aspartic acid, and its activities were confirmed by three classical cytokinin bioassays as well as by using binding and activation assays with the Arabidopsis cytokinin receptors AHK3 and CRE1/AHK4.
[Mh] Termos MeSH primário: Adenina/análogos & derivados
Arabidopsis/metabolismo
Dictyostelium/química
[Mh] Termos MeSH secundário: Adenina/síntese química
Adenina/química
Adenina/farmacologia
Ácido Aspártico/química
Citocininas/química
Citocininas/metabolismo
Estrutura Molecular
Estereoisomerismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokinins); 30KYC7MIAI (Aspartic Acid); 62061-49-8 (discadenine); JAC85A2161 (Adenine)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170701
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jnatprod.6b01165


  6 / 6906 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28636923
[Au] Autor:Copos CA; Walcott S; Del Álamo JC; Bastounis E; Mogilner A; Guy RD
[Ad] Endereço:Department of Mathematics, University of California Davis, Davis, California. Electronic address: ccopos@math.ucdavis.edu.
[Ti] Título:Mechanosensitive Adhesion Explains Stepping Motility in Amoeboid Cells.
[So] Source:Biophys J;112(12):2672-2682, 2017 Jun 20.
[Is] ISSN:1542-0086
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cells employing amoeboid motility exhibit repetitive cycles of rapid expansion and contraction and apply coordinated traction forces to their environment. Although aspects of this process are well studied, it is unclear how the cell controls the coordination of cell length changes with adhesion to the surface. Here, we develop a simple model to mechanistically explain the emergence of periodic changes in length and spatiotemporal dynamics of traction forces measured in chemotaxing unicellular amoeba, Dictyostelium discoideum. In contrast to the biochemical mechanisms that have been implicated in the coordination of some cellular processes, we show that many features of amoeboid locomotion emerge from a simple mechanochemical model. The mechanism for interaction with the environment in Dictyostelium is unknown and thus, we explore different cell-environment interaction models to reveal that mechanosensitive adhesions are necessary to reproduce the spatiotemporal adhesion patterns. In this modeling framework, we find that the other motility modes, such as smooth gliding, arise naturally with variations in the physical properties of the surface. Thus, our work highlights the prominent role of biomechanics in determining the emergent features of amoeboid locomotion.
[Mh] Termos MeSH primário: Adesão Celular/fisiologia
Dictyostelium/fisiologia
Mecanotransdução Celular/fisiologia
[Mh] Termos MeSH secundário: Actinas/metabolismo
Actomiosina/metabolismo
Membrana Celular/fisiologia
Citoesqueleto/fisiologia
Citosol/metabolismo
Meio Ambiente
Modelos Biológicos
Movimento/fisiologia
Polimerização
Propriedades de Superfície
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Actins); 9013-26-7 (Actomyosin)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170823
[Lr] Data última revisão:
170823
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170622
[St] Status:MEDLINE


  7 / 6906 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28628084
[Au] Autor:Kronenberg NM; Liehm P; Steude A; Knipper JA; Borger JG; Scarcelli G; Franze K; Powis SJ; Gather MC
[Ad] Endereço:SUPA, School of Physics and Astronomy, University of St Andrews, St Andrews KY16 9SS, UK.
[Ti] Título:Long-term imaging of cellular forces with high precision by elastic resonator interference stress microscopy.
[So] Source:Nat Cell Biol;19(7):864-872, 2017 Jul.
[Is] ISSN:1476-4679
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Cellular forces are crucial for many biological processes but current methods to image them have limitations with respect to data analysis, resolution and throughput. Here, we present a robust approach to measure mechanical cell-substrate interactions in diverse biological systems by interferometrically detecting deformations of an elastic micro-cavity. Elastic resonator interference stress microscopy (ERISM) yields stress maps with exceptional precision and large dynamic range (2 nm displacement resolution over a >1 µm range, translating into 1 pN force sensitivity). This enables investigation of minute vertical stresses (<1 Pa) involved in podosome protrusion, protein-specific cell-substrate interaction and amoeboid migration through spatial confinement in real time. ERISM requires no zero-force reference and avoids phototoxic effects, which facilitates force monitoring over multiple days and at high frame rates and eliminates the need to detach cells after measurements. This allows observation of slow processes such as differentiation and further investigation of cells, for example, by immunostaining.
[Mh] Termos MeSH primário: Movimento Celular
Dictyostelium/fisiologia
Fibroblastos/fisiologia
Macrófagos/fisiologia
Microscopia de Interferência/métodos
Podossomos/fisiologia
Linfócitos T/fisiologia
[Mh] Termos MeSH secundário: Células 3T3
Animais
Fenômenos Biomecânicos
Adesão Celular
Dictyostelium/metabolismo
Elasticidade
Matriz Extracelular/metabolismo
Fibroblastos/metabolismo
Seres Humanos
Macrófagos/metabolismo
Camundongos
Camundongos Endogâmicos BALB C
Camundongos Transgênicos
Microscopia de Vídeo
Podossomos/metabolismo
Estresse Mecânico
Linfócitos T/metabolismo
Fatores de Tempo
Imagem com Lapso de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; VIDEO-AUDIO MEDIA
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170913
[Lr] Data última revisão:
170913
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170620
[St] Status:MEDLINE
[do] DOI:10.1038/ncb3561


  8 / 6906 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28578698
[Au] Autor:Stajdohar M; Rosengarten RD; Kokosar J; Jeran L; Blenkus D; Shaulsky G; Zupan B
[Ad] Endereço:Genialis d.o.o., Trzaska cesta 315, Ljubljana, 1000, Slovenia.
[Ti] Título:dictyExpress: a web-based platform for sequence data management and analytics in Dictyostelium and beyond.
[So] Source:BMC Bioinformatics;18(1):291, 2017 Jun 02.
[Is] ISSN:1471-2105
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Dictyostelium discoideum, a soil-dwelling social amoeba, is a model for the study of numerous biological processes. Research in the field has benefited mightily from the adoption of next-generation sequencing for genomics and transcriptomics. Dictyostelium biologists now face the widespread challenges of analyzing and exploring high dimensional data sets to generate hypotheses and discovering novel insights. RESULTS: We present dictyExpress (2.0), a web application designed for exploratory analysis of gene expression data, as well as data from related experiments such as Chromatin Immunoprecipitation sequencing (ChIP-Seq). The application features visualization modules that include time course expression profiles, clustering, gene ontology enrichment analysis, differential expression analysis and comparison of experiments. All visualizations are interactive and interconnected, such that the selection of genes in one module propagates instantly to visualizations in other modules. dictyExpress currently stores the data from over 800 Dictyostelium experiments and is embedded within a general-purpose software framework for management of next-generation sequencing data. dictyExpress allows users to explore their data in a broader context by reciprocal linking with dictyBase-a repository of Dictyostelium genomic data. In addition, we introduce a companion application called GenBoard, an intuitive graphic user interface for data management and bioinformatics analysis. CONCLUSIONS: dictyExpress and GenBoard enable broad adoption of next generation sequencing based inquiries by the Dictyostelium research community. Labs without the means to undertake deep sequencing projects can mine the data available to the public. The entire information flow, from raw sequence data to hypothesis testing, can be accomplished in an efficient workspace. The software framework is generalizable and represents a useful approach for any research community. To encourage more wide usage, the backend is open-source, available for extension and further development by bioinformaticians and data scientists.
[Mh] Termos MeSH primário: Dictyostelium/metabolismo
Interface Usuário-Computador
[Mh] Termos MeSH secundário: Imunoprecipitação da Cromatina
Análise por Conglomerados
Dictyostelium/genética
Sequenciamento de Nucleotídeos em Larga Escala
Internet
Análise de Sequência de RNA
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170606
[St] Status:MEDLINE
[do] DOI:10.1186/s12859-017-1706-9


  9 / 6906 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28556908
[Au] Autor:Hykollari A; Malzl D; Yan S; Wilson IBH; Paschinger K
[Ad] Endereço:Department für Chemie, Universität für Bodenkultur, Wien, Austria.
[Ti] Título:Hydrophilic interaction anion exchange for separation of multiply modified neutral and anionic Dictyostelium N-glycans.
[So] Source:Electrophoresis;38(17):2175-2183, 2017 09.
[Is] ISSN:1522-2683
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The unusual nature of the N-glycans of the cellular slime mould Dictyostelium discoideum has been revealed by a number of studies, primarily based on examination of radiolabeled glycopeptides but more recently also by MS. The complexity of the N-glycomes of even glycosylation mutants is compounded by the occurrence of anionic modifications, which also present an analytical challenge. In this study, we have employed hydrophilic interaction anion exchange (HIAX) HPLC in combination with MALDI-TOF MS/MS to explore the anionic N-glycome of the M31 (modA) strain, which lacks endoplasmic reticulum α-glucosidase II, an enzyme conserved in most eukaryotes including Homo sapiens. Prefractionation with HIAX chromatography enabled the identification of N-glycans with unusual oligo-α1,2-mannose extensions as well as others with up to four anionic modifications. Due to the use of hydrofluoric acid treatment, we were able to discriminate isobaric glycans differing in the presence of sulphate or phosphate on intersected structures as opposed to those carrying GlcNAc-phosphodiesters. The latter represent biosynthetic intermediates during the pathway leading to formation of the methylphosphorylated mannose epitope, which may have a similar function in intracellular targeting of hydrolases as the mannose-6-phosphate modification of lysosomal enzymes in mammals. In conclusion, HIAX in combination with MS is a highly sensitive approach for both fine separation and definition of neutral and anionic N-glycan structures.
[Mh] Termos MeSH primário: Cromatografia por Troca Iônica/métodos
Dictyostelium/química
Glicômica/métodos
Polissacarídeos/análise
Polissacarídeos/química
[Mh] Termos MeSH secundário: Cromatografia Líquida de Alta Pressão/métodos
Dictyostelium/metabolismo
Hexoses/análise
Hexoses/química
Interações Hidrofóbicas e Hidrofílicas
Manose/análise
Manose/química
Fosfatos/análise
Fosfatos/química
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos
Sulfatos/análise
Sulfatos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Hexoses); 0 (Phosphates); 0 (Polysaccharides); 0 (Sulfates); PHA4727WTP (Mannose)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170531
[St] Status:MEDLINE
[do] DOI:10.1002/elps.201700073


  10 / 6906 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28512027
[Au] Autor:Verhasselt S; Stevens CV; Van den Broecke T; Bracke ME; Roman BI
[Ad] Endereço:SynBioC Research Group, Department of Sustainable Organic Chemistry and Technology, Campus Coupure, Ghent University, Coupure Links 653, 9000 Ghent, Belgium.
[Ti] Título:Insights into the myosin II inhibitory potency of A-ring-modified (S)-blebbistatin analogs.
[So] Source:Bioorg Med Chem Lett;27(13):2986-2989, 2017 07 01.
[Is] ISSN:1464-3405
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Myosin II is an interesting target for therapeutic intervention, as it is involved in a large number of motility-based diseases. (S)-Blebbistatin is a known micromolar inhibitor of this protein. A new series of (S)-blebbistatin derivatives with a modified A-ring was synthesized and the myosin II inhibitory properties were evaluated in vitro. In this way, we gained insight into the influence of structural modifications in this part of the scaffold on myosin II inhibitory potency. Our results indicate there are few possibilities for potency enhancement via ring A modification of the blebbistatin scaffold.
[Mh] Termos MeSH primário: Dictyostelium/enzimologia
Inibidores Enzimáticos/farmacologia
Compostos Heterocíclicos de 4 ou mais Anéis/farmacologia
Miosina Tipo II/antagonistas & inibidores
[Mh] Termos MeSH secundário: Relação Dose-Resposta a Droga
Inibidores Enzimáticos/síntese química
Inibidores Enzimáticos/química
Compostos Heterocíclicos de 4 ou mais Anéis/síntese química
Compostos Heterocíclicos de 4 ou mais Anéis/química
Estrutura Molecular
Miosina Tipo II/metabolismo
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Enzyme Inhibitors); 0 (Heterocyclic Compounds, 4 or More Rings); 20WC4J7CQ6 (blebbistatin); EC 3.6.1.- (Myosin Type II)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171124
[Lr] Data última revisão:
171124
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170518
[St] Status:MEDLINE



página 1 de 691 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde