Base de dados : MEDLINE
Pesquisa : B01.046.550.550.600 [Categoria DeCS]
Referências encontradas : 5 [refinar]
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[PMID]:21195500
[Au] Autor:Michalczyk D; Drozdowicz A; Pintscher S; Plonka PM
[Ad] Endereço:Department of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, ul Gronostajowa 7, 30-387 Kraków, Poland.
[Ti] Título:Mycetozoal bloom in a hydroponic culture of garden cress (Lepidium sativum L.).
[So] Source:Int J Food Microbiol;145(1):336-41, 2011 Jan 31.
[Is] ISSN:1879-3460
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The role and importance of the true slime moulds (mycetozoans, Mycetozoa, formerly Myxomycetes) for agriculture and food industry are poorly documented, most probably because of a low popularity of these "macroscopic microorganisms" among researchers in the past. Here we report probably for the first time the massive occurrence of true slime moulds in their vegetative, plasmodial form in a product intended for direct consumption, i.e. in a household hydroponic culture of garden cress (Lepidium sativum L.). The plasmodia gradually produced numerous, stalked or sessile sporangia and plasmodiocarps, which made it possible to identify them as Didymium species complex (the stalked sporangia) and Didymium difforme (Pers.) Gray (the sessile sporangia and plasmodiocarps). The mycetozoans were transferred to in vitro culture where they were maintained for several weeks on oat flour. We briefly discuss the importance of this observation from the point of view of biochemical interactions between the plant and the true slime moulds in a general context of slime mould biology. Our observation indicates that the presence of mycetozoan material in food products may be frequent, while its influence on food safety and quality remains unknown.
[Mh] Termos MeSH primário: Contaminação de Alimentos
Microbiologia de Alimentos
Lepidium sativum/microbiologia
Physarida/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Hidroponia
Physarida/isolamento & purificação
Esporângios/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1105
[Cu] Atualização por classe:110221
[Lr] Data última revisão:
110221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:110104
[St] Status:MEDLINE
[do] DOI:10.1016/j.ijfoodmicro.2010.11.042


  2 / 5 MEDLINE  
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[PMID]:17965965
[Au] Autor:Krzywda A; Petelenz E; Michalczyk D; Plonka PM
[Ad] Endereço:Department of Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, ul. Gronostajowa 7, 30-387, Kraków, Poland.
[Ti] Título:Sclerotia of the acellular (true) slime mould Fuligo septica as a model to study melanization and anabiosis.
[So] Source:Cell Mol Biol Lett;13(1):130-43, 2008.
[Is] ISSN:1689-1392
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Acellular (true) slime moulds (Myxomycetes) are capable of a transition to the stage of sclerotium - a dormant form of plasmodium produced under unfavourable environmental conditions. In this study, sclerotia of Fuligo septica were analyzed by means of electron paramagnetic resonance (EPR) spectroscopy. The moulds were cultivated in vitro on filter paper, fed with oat flour, and kept until the plasmodia began to produce sclerotia. The obtained sclerotia differed in colour from yellow through orange to dark-brown. The EPR spectra revealed a free radical, melanin-like signal correlated with the depth of the colour; it was strongest in the dark sclerotia. Sclerotization only took place when the plasmodia were starved and very slowly dried. Only the yellow sclerotia were able to regenerate into viable plasmodia. This suggests that myxomycete cytoplasm dehydration is an active process regulated metabolically. Plasmodial sclerotization may therefore serve as a convenient model system to study the regulation of cytoplasmatic water balance, and sclerotia as a convenient material for EPR measurements, combining the quality of plasmodia with the technical simplicity of the measurements characteristic of dry spores. Darkening of the sclerotia is most probably a pathological phenomenon connected with the impairment of water balance during sclerotization.
[Mh] Termos MeSH primário: Melaninas/metabolismo
Modelos Biológicos
Micélio/metabolismo
Physarida/metabolismo
Esporos de Protozoários/metabolismo
[Mh] Termos MeSH secundário: Animais
Espectroscopia de Ressonância de Spin Eletrônica
Melaninas/química
Micélio/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Melanins)
[Em] Mês de entrada:0806
[Cu] Atualização por classe:071231
[Lr] Data última revisão:
071231
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:071030
[St] Status:MEDLINE


  3 / 5 MEDLINE  
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[PMID]:18065011
[Au] Autor:Lado C; Mosquera J; Estrada-Torres A; Beltrán-Tejera E; de Basanta DW
[Ad] Endereço:Real Jardín Botánico, CSIC, Plaza de Murillo 2, 28014 Madrid, España. lado@rjb.csic.es
[Ti] Título:Description and culture of a new succulenticolous Didymium (Myxomycetes).
[So] Source:Mycologia;99(4):602-11, 2007 Jul-Aug.
[Is] ISSN:0027-5514
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A new succulenticolous Myxomycete species, Didymium wildpretii, found on decaying remains of various species of cacti, is described from two arid zones of the world. This species was collected from central Mexico, at the southern limit of the Chihuahuan Desert, and from the Canary Islands (Spain). The new species has small, pale yellow sporocarps, 0.1-0.7 mm high, that are sessile or have short, orange-yellow, calcareous stalks and small, uniformly warted spores. The stability of the taxonomic characters of the species was confirmed with both moist chamber cultures and spore-to-spore culture on agar. Life cycle events are described from germination to sporulation. Myxomycete specimens were examined with scanning electron microscopy and light microscopy, and micrographs of relevant morphological details are included.
[Mh] Termos MeSH primário: Cactaceae/microbiologia
Physarida/classificação
[Mh] Termos MeSH secundário: Ágar
Animais
Meios de Cultura
Clima Desértico
México
Microscopia Eletrônica de Varredura
Physarida/crescimento & desenvolvimento
Physarida/isolamento & purificação
Physarida/fisiologia
Espanha
Especificidade da Espécie
Esporos Fúngicos/fisiologia
Esporos Fúngicos/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Culture Media); 9002-18-0 (Agar)
[Em] Mês de entrada:0801
[Cu] Atualização por classe:071210
[Lr] Data última revisão:
071210
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:071211
[St] Status:MEDLINE


  4 / 5 MEDLINE  
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[PMID]:17361110
[Au] Autor:Johansen SD; Vader A; Sjøttem E; Nielsen H
[Ad] Endereço:Department of Molecular Biotechnology, Institute of Medical Biology, University of Tromsø, Tromsø, Norway. Steinar.Johansen@fagmed.uit.no
[Ti] Título:In vivo expression of a group I intron HEG from the antisense strand of Didymium ribosomal DNA.
[So] Source:RNA Biol;3(4):157-62, 2006 Oct.
[Is] ISSN:1555-8584
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Two different isolates of the myxomycete Didymium iridis harbour homing endonuclease genes that are expressed from group I introns inserted into identical sites within the small subunit ribosomal DNA. The homing endonuclease proteins are related in sequence, and their gene structures share similar features such as the presence of small spliceosomal introns and functional polyadenylation sites. However, they are transcribed from opposite strands of the ribosomal DNA and presumable by different RNA polymerases. We have previously described the in vivo expression of the I-DirI homing endonuclease from within the ribosomal RNA precursor. In this paper, we demonstrate the in vivo expression of the I-DirII homing endonuclease from the opposite strand of the Didymium rRNA gene. A comparison of the expression strategies of the two genes demonstrates the feasibility of antisense expression and provides insight into nucleolar gene expression.
[Mh] Termos MeSH primário: DNA Antissenso/genética
DNA Ribossômico/genética
Endodesoxirribonucleases/genética
Íntrons/genética
Physarida/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Sequência de Bases
Dados de Sequência Molecular
Physarida/enzimologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Antisense); 0 (DNA, Ribosomal); EC 3.1.- (Endodeoxyribonucleases)
[Em] Mês de entrada:0705
[Cu] Atualização por classe:100115
[Lr] Data última revisão:
100115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:070316
[St] Status:MEDLINE


  5 / 5 MEDLINE  
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[PMID]:16800304
[Au] Autor:Scheer MA; Silliker ME
[Ad] Endereço:Nova Southeastern University College of Osteopathic Medicine, Fort Lauderdale, Florida 33328, USA.
[Ti] Título:Mitochondrial inheritance patterns in Didymium iridis are not influenced by stage of mating competency.
[So] Source:Mycologia;98(1):51-6, 2006 Jan-Feb.
[Is] ISSN:0027-5514
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:To test whether the timing of transition to mating competency affected mitochondrial transmission patterns in D. iridis. Reciprocal crosses were made by combining mating compatible strains that differed in their competency to mate. The results were compared to crosses where both mating strains were competent at the time of combining and crosses where somatic fusion of plasmodia was allowed. The results show that the mating competency of the parental strains at the time of confronting a compatible mate does not affect mitochondrial transmission patterns, mating efficiency or the likelihood of biparental inheritance. However the timing of plasmodial formation is delayed when precompetent and competent strains are mated compared to when both strains are competent at the time of mixing. We also observed that somatic fusion of plasmodia did not appreciably increase the incidence of biparental inheritance compared to crosses where individual plasmodia were isolated. These results provide additional evidence of the variable nature of mitochondrial inheritance in D. iridis within crosses and between mating trials.
[Mh] Termos MeSH primário: Genes Mitocondriais
Physarida/genética
[Mh] Termos MeSH secundário: Animais
Cruzamentos Genéticos
DNA Mitocondrial/genética
DNA de Protozoário/genética
Mitocôndrias/fisiologia
Hibridização de Ácido Nucleico
Physarida/citologia
Physarida/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Mitochondrial); 0 (DNA, Protozoan)
[Em] Mês de entrada:0608
[Cu] Atualização por classe:061115
[Lr] Data última revisão:
061115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:060628
[St] Status:MEDLINE



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