Base de dados : MEDLINE
Pesquisa : B01.046.550.550.600.700 [Categoria DeCS]
Referências encontradas : 1017 [refinar]
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  1 / 1017 MEDLINE  
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[PMID]:28182547
[Au] Autor:Liu Y; Gao C; Zhang Z; Lu Y; Chen S; Liang M; Tao L
[Ti] Título:Solving NP-Hard Problems with Physarum-Based Ant Colony System.
[So] Source:IEEE/ACM Trans Comput Biol Bioinform;14(1):108-120, 2017 Jan-Feb.
[Is] ISSN:1557-9964
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:NP-hard problems exist in many real world applications. Ant colony optimization (ACO) algorithms can provide approximate solutions for those NP-hard problems, but the performance of ACO algorithms is significantly reduced due to premature convergence and weak robustness, etc. With these observations in mind, this paper proposes a Physarum-based pheromone matrix optimization strategy in ant colony system (ACS) for solving NP-hard problems such as traveling salesman problem (TSP) and 0/1 knapsack problem (0/1 KP). In the Physarum-inspired mathematical model, one of the unique characteristics is that critical tubes can be reserved in the process of network evolution. The optimized updating strategy employs the unique feature and accelerates the positive feedback process in ACS, which contributes to the quick convergence of the optimal solution. Some experiments were conducted using both benchmark and real datasets. The experimental results show that the optimized ACS outperforms other meta-heuristic algorithms in accuracy and robustness for solving TSPs. Meanwhile, the convergence rate and robustness for solving 0/1 KPs are better than those of classical ACS.
[Mh] Termos MeSH primário: Algoritmos
Formigas/fisiologia
Biomimética/métodos
Técnicas de Apoio para a Decisão
Feromônios/metabolismo
Physarum/fisiologia
[Mh] Termos MeSH secundário: Animais
Simulação por Computador
Modelos Biológicos
Modelos Estatísticos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Pheromones)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170210
[St] Status:MEDLINE
[do] DOI:10.1109/TCBB.2015.2462349


  2 / 1017 MEDLINE  
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[PMID]:27862633
[Au] Autor:Gao Y; Tao W; Yan SZ; Chen SL
[Ad] Endereço:College of Life Sciences, Nanjing Normal University, Nanjing, Jiangsu, 210023, China.
[Ti] Título:The Life Cycle of Didymium laxifilum and Physarum album on Oat Agar Culture.
[So] Source:J Eukaryot Microbiol;64(4):457-463, 2017 Jul.
[Is] ISSN:1550-7408
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The plasmodial slime molds is the largest group in the phylum Amoebozoa. Its life cycle includes the plasmodial trophic stage and the spore-bearing fruiting bodies. However, only a few species have their complete life cycle known in details so far. This study is the first reporting the morphogenesis of Didymium laxifilum and Physarum album. Spores, from field-collected sporangia, were incubated into hanging drop cultures for viewing germination and axenic oat agar plates for viewing plasmodial development and sporulation. The spores of D. laxifilum and P. album germinated by method of V-shape split and minute pore, respectively. The amoeboflagellates, released from spores, were observed in water film. The phaneroplasmodia of two species developed into a number of sporangia by subhypothallic type on oat agar culture. The main interspecific difference of morphogenesis was also discussed.
[Mh] Termos MeSH primário: Estágios do Ciclo de Vida/fisiologia
Mixomicetos/crescimento & desenvolvimento
Physarum/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Cultura Axênica
Microscopia Eletrônica de Varredura
Morfogênese
Mixomicetos/classificação
Physarum/classificação
Esporos de Protozoários/crescimento & desenvolvimento
Madeira/parasitologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170919
[Lr] Data última revisão:
170919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161119
[St] Status:MEDLINE
[do] DOI:10.1111/jeu.12383


  3 / 1017 MEDLINE  
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[PMID]:28105946
[Au] Autor:Sun Y; Hameed PN; Verspoor K; Halgamuge S
[Ad] Endereço:Department of Mechanical Engineering, University of Melbourne, Parkville, Melbourne, 3010, Australia.
[Ti] Título:A physarum-inspired prize-collecting steiner tree approach to identify subnetworks for drug repositioning.
[So] Source:BMC Syst Biol;10(Suppl 5):128, 2016 Dec 05.
[Is] ISSN:1752-0509
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Drug repositioning can reduce the time, costs and risks of drug development by identifying new therapeutic effects for known drugs. It is challenging to reposition drugs as pharmacological data is large and complex. Subnetwork identification has already been used to simplify the visualization and interpretation of biological data, but it has not been applied to drug repositioning so far. In this paper, we fill this gap by proposing a new Physarum-inspired Prize-Collecting Steiner Tree algorithm to identify subnetworks for drug repositioning. RESULTS: Drug Similarity Networks (DSN) are generated using the chemical, therapeutic, protein, and phenotype features of drugs. In DSNs, vertex prizes and edge costs represent the similarities and dissimilarities between drugs respectively, and terminals represent drugs in the cardiovascular class, as defined in the Anatomical Therapeutic Chemical classification system. A new Physarum-inspired Prize-Collecting Steiner Tree algorithm is proposed in this paper to identify subnetworks. We apply both the proposed algorithm and the widely-used GW algorithm to identify subnetworks in our 18 generated DSNs. In these DSNs, our proposed algorithm identifies subnetworks with an average Rand Index of 81.1%, while the GW algorithm can only identify subnetworks with an average Rand Index of 64.1%. We select 9 subnetworks with high Rand Index to find drug repositioning opportunities. 10 frequently occurring drugs in these subnetworks are identified as candidates to be repositioned for cardiovascular diseases. CONCLUSIONS: We find evidence to support previous discoveries that nitroglycerin, theophylline and acarbose may be able to be repositioned for cardiovascular diseases. Moreover, we identify seven previously unknown drug candidates that also may interact with the biological cardiovascular system. These discoveries show our proposed Prize-Collecting Steiner Tree approach as a promising strategy for drug repositioning.
[Mh] Termos MeSH primário: Algoritmos
Biomimética
Biologia Computacional/métodos
Reposicionamento de Medicamentos
Physarum
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170121
[St] Status:MEDLINE
[do] DOI:10.1186/s12918-016-0371-3


  4 / 1017 MEDLINE  
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[PMID]:27125976
[Au] Autor:Zhang Y; Kawamichi H; Kohama K; Nakamura A
[Ad] Endereço:Department of Molecular Physiology and Medical Bioregulation, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi 755-8505, Japan.
[Ti] Título:Calcium-mediated regulation of recombinant hybrids of full-length Physarum myosin heavy chain with Physarum/scallop myosin light chains.
[So] Source:Acta Biochim Biophys Sin (Shanghai);48(6):536-43, 2016 Jun.
[Is] ISSN:1745-7270
[Cp] País de publicação:China
[La] Idioma:eng
[Ab] Resumo:Physarum myosin is a Ca(2+)-binding protein and its activity is inhibited by Ca(2+) In the present study, to clarify the light chains (LCs) from the different species (Physarum and scallop) and to determine the specific Ca(2+)-regulated effects, we constructed hybrid myosins with a Physarum myosin heavy chain (Ph·HC) and Physarum and/or scallop myosin LCs, and examined Ca(2+)-mediated regulation of ATPases and motor activities. In these experiments, it was found that Ca(2+) inhibited motilities and ATPase activities of Physarum hybrid myosin with scallop regulatory light chain (ScRLC) and Physarum essential light chain (PhELC) but could not inhibit those of the Physarum hybrid myosin mutant Ph·HC/ScRLC/PhELC-3A which lacks Ca(2+)-binding ability, indicating that PhELC plays a critical role in Ca(2+)-mediated regulation of Physarum myosin. Furthermore, the effects of Ca(2+) on ATPase activities of Physarum myosin constructs are in the following order: Ph·HC/PhRLC/PhELC > Ph·HC/ScRLC/PhELC > Ph·HC/PhRLC/ScELC > Ph·HC/ScRLC/ScELC, suggesting that the presence of PhRLC and PhELC leads to the greatest Ca(2+) sensitivity of Physarum myosin. Although we did not observe the motilities of Physarum hybrid myosin Ph·HC/PhRLC/ScELC and Ph·HC/ScRLC/ScELC, our results suggest that Ca(2+)-binding to the PhELC may alter the flexibility of the regulatory domain and induce a 'closed' state, which may consequently prevent full activity and force generation.
[Mh] Termos MeSH primário: Cadeias Pesadas de Miosina/metabolismo
Cadeias Leves de Miosina/metabolismo
Pectinidae/metabolismo
Physarum/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Fenômenos Biofísicos
Cálcio/metabolismo
Modelos Moleculares
Movimento
Cadeias Pesadas de Miosina/química
Cadeias Pesadas de Miosina/genética
Cadeias Leves de Miosina/química
Cadeias Leves de Miosina/genética
Pectinidae/genética
Physarum/genética
Proteínas de Protozoários/química
Proteínas de Protozoários/genética
Proteínas de Protozoários/metabolismo
Proteínas Recombinantes de Fusão/química
Proteínas Recombinantes de Fusão/genética
Proteínas Recombinantes de Fusão/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Myosin Light Chains); 0 (Protozoan Proteins); 0 (Recombinant Fusion Proteins); EC 3.6.4.1 (Myosin Heavy Chains); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170601
[Lr] Data última revisão:
170601
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160430
[St] Status:MEDLINE
[do] DOI:10.1093/abbs/gmw031


  5 / 1017 MEDLINE  
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[PMID]:26751562
[Au] Autor:Zhang Z; Gao C; Lu Y; Liu Y; Liang M
[Ad] Endereço:College of Computer and Information Science & College of Software, Southwest University, Chongqing 400715, China.
[Ti] Título:Multi-Objective Ant Colony Optimization Based on the Physarum-Inspired Mathematical Model for Bi-Objective Traveling Salesman Problems.
[So] Source:PLoS One;11(1):e0146709, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bi-objective Traveling Salesman Problem (bTSP) is an important field in the operations research, its solutions can be widely applied in the real world. Many researches of Multi-objective Ant Colony Optimization (MOACOs) have been proposed to solve bTSPs. However, most of MOACOs suffer premature convergence. This paper proposes an optimization strategy for MOACOs by optimizing the initialization of pheromone matrix with the prior knowledge of Physarum-inspired Mathematical Model (PMM). PMM can find the shortest route between two nodes based on the positive feedback mechanism. The optimized algorithms, named as iPM-MOACOs, can enhance the pheromone in the short paths and promote the search ability of ants. A series of experiments are conducted and experimental results show that the proposed strategy can achieve a better compromise solution than the original MOACOs for solving bTSPs.
[Mh] Termos MeSH primário: Formigas/fisiologia
Physarum/metabolismo
[Mh] Termos MeSH secundário: Algoritmos
Animais
Comportamento Animal
Simulação por Computador
Modelos Biológicos
Modelos Teóricos
Feromônios/química
Resolução de Problemas
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Pheromones)
[Em] Mês de entrada:1607
[Cu] Atualização por classe:160116
[Lr] Data última revisão:
160116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160112
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0146709


  6 / 1017 MEDLINE  
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[PMID]:26518741
[Au] Autor:Stanley-Wall NR; Coulthurst SJ; Holland IB
[Ad] Endereço:Division of Molecular Microbiology of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom. Electronic address: N.R.Stanleywall@dundee.ac.uk.
[Ti] Título:A Snapshot of the Extraordinary World of Social Microbiology.
[So] Source:J Mol Biol;427(23):3625-7, 2015 Nov 20.
[Is] ISSN:1089-8638
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Consórcios Microbianos/fisiologia
[Mh] Termos MeSH secundário: Biofilmes
Flagelos
Seres Humanos
Boca/microbiologia
Myxococcales/fisiologia
Physarum/fisiologia
Pseudomonas aeruginosa/patogenicidade
Percepção de Quorum
[Pt] Tipo de publicação:EDITORIAL; INTRODUCTORY JOURNAL ARTICLE
[Em] Mês de entrada:1605
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151101
[St] Status:MEDLINE


  7 / 1017 MEDLINE  
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[PMID]:25311445
[Au] Autor:Michel R; Walochnik J; Scheid P
[Ad] Endereço:Central Institute of the Federal Armed Forces Medical Services, Department of Parasitology, Andernacher Straße 100, Koblenz D-56070, Germany. Electronic address: rolf.michel@email.de.
[Ti] Título:Article for the "Free-living amoebae special issue": Isolation and characterisation of various amoebophagous fungi and evaluation of their prey spectrum.
[So] Source:Exp Parasitol;145 Suppl:S131-6, 2014 Nov.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This article gives an overview on the isolation and characterisation of endoparasitic fungi invading free-living amoebae (FLA), including the ones forming thalli inside their hosts such as Cochlonema euryblastum and also the predatory fungi which capture amoebae by adhesive hyphae. Acaulopage spp. and Stylopage spp. trap, intrude, and exploit amoebal trophozoites. Previous phylogenetic studies proved Cochlonema to be a member of the Zoopagales. The genetic investigation of Acaulopage tetraceros demonstrated its close relationship to Cochlonema. Co-cultivation of A. tetraceros with a number of FLA revealed a great prey spectrum of this amoebophageous fungus. In addition it was shown that solitary amoebal stages of slime moulds such as Dictyostelium sp. and Physarum sp. are also suited as welcome prey amoebae.
[Mh] Termos MeSH primário: Amoeba/microbiologia
Fungos/isolamento & purificação
Fungos/fisiologia
[Mh] Termos MeSH secundário: Amoeba/ultraestrutura
Compostos Azo
Benzenossulfonatos
Corantes
DNA Fúngico/química
DNA Fúngico/isolamento & purificação
DNA Ribossômico/química
Dictyostelium/isolamento & purificação
Dictyostelium/fisiologia
Amarelo de Eosina-(YS)
Corantes Fluorescentes
Fungos/classificação
Fungos/ultraestrutura
Verde de Metila
Microscopia Eletrônica de Transmissão
Dados de Sequência Molecular
Filogenia
Physarum/isolamento & purificação
Physarum/fisiologia
RNA Ribossômico 18S/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Azo Compounds); 0 (Benzenesulfonates); 0 (Coloring Agents); 0 (DNA, Fungal); 0 (DNA, Ribosomal); 0 (Fluorescent Dyes); 0 (RNA, Ribosomal, 18S); 0 (trichrome stain); 7S9P0Y4313 (C.I. Fluorescent Brightening Agent 28); 82-94-0 (Methyl Green); TDQ283MPCW (Eosine Yellowish-(YS))
[Em] Mês de entrada:1502
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141015
[St] Status:MEDLINE


  8 / 1017 MEDLINE  
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[PMID]:25200281
[Au] Autor:Le Cigne A; Menil-Philippot V; Fleury F; Takahashi M; Thiriet C
[Ad] Endereço:Faculté des Sciences et des Techniques, UFIP UMR CNRS 6286 & Université de Nantes, 44322, Nantes Cedex 3, France; Division of Mechanism and Regulation of DNA Repair, Faculté des Sciences et des Techniques, UFIP UMR CNRS 6286 & Université de Nantes, 44322, Nantes Cedex 3, France; Division of Epigenetics: Proliferation and Differentiation, Faculté des Sciences et des Techniques, UFIP UMR CNRS 6286 & Université de Nantes, 44322, Nantes Cedex 3, France.
[Ti] Título:Transient expression of RAD51 in the late G2-phase is required for cell cycle progression in synchronous Physarum cells.
[So] Source:Genes Cells;19(10):755-65, 2014 Oct.
[Is] ISSN:1365-2443
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The homologous recombination factor RAD51 is highly conserved. This criterion enabled us to identify a RAD51 ortholog in Physarum polycephalum. We found that the Physarum protein presents a high homology to the human protein and cross-reacted with antibodies directed against the human RAD51. Taking advantage of the natural synchrony of millions of nuclei within a single cell of Physarum, we investigated the fluctuation of the amount of the PpRAD51 throughout the cell cycle. Our results showed that in the late G2-phase, RAD51 was transiently expressed in a large quantity. Furthermore, knocking-down RAD51 in the G2-phase abolished this transient expression before mitosis and affected cell cycle progression. These results support the idea that RAD51 plays a role in the progression of the cell cycle in the late G2-phase.
[Mh] Termos MeSH primário: Fase G2
Physarum/metabolismo
Rad51 Recombinase/metabolismo
[Mh] Termos MeSH secundário: Seres Humanos
Physarum/citologia
RNA Interferente Pequeno/metabolismo
Rad51 Recombinase/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Small Interfering); EC 2.7.7.- (Rad51 Recombinase)
[Em] Mês de entrada:1505
[Cu] Atualização por classe:140926
[Lr] Data última revisão:
140926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140910
[St] Status:MEDLINE
[do] DOI:10.1111/gtc.12174


  9 / 1017 MEDLINE  
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[PMID]:24586616
[Au] Autor:Watanabe S; Takamatsu A
[Ad] Endereço:Department of Electrical Engineering and Bioscience, Waseda University, Shinjuku-ku, Tokyo, Japan.
[Ti] Título:Transportation network with fluctuating input/output designed by the bio-inspired Physarum algorithm.
[So] Source:PLoS One;9(2):e89231, 2014.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In this paper, we propose designing transportation network topology and traffic distribution under fluctuating conditions using a bio-inspired algorithm. The algorithm is inspired by the adaptive behavior observed in an amoeba-like organism, plasmodial slime mold, more formally known as plasmodium of Physarum plycephalum. This organism forms a transportation network to distribute its protoplasm, the fluidic contents of its cell, throughout its large cell body. In this process, the diameter of the transportation tubes adapts to the flux of the protoplasm. The Physarum algorithm, which mimics this adaptive behavior, has been widely applied to complex problems, such as maze solving and designing the topology of railroad grids, under static conditions. However, in most situations, environmental conditions fluctuate; for example, in power grids, the consumption of electric power shows daily, weekly, and annual periodicity depending on the lifestyles or the business needs of the individual consumers. This paper studies the design of network topology and traffic distribution with oscillatory input and output traffic flows. The network topology proposed by the Physarum algorithm is controlled by a parameter of the adaptation process of the tubes. We observe various rich topologies such as complete mesh, partial mesh, Y-shaped, and V-shaped networks depending on this adaptation parameter and evaluate them on the basis of three performance functions: loss, cost, and vulnerability. Our results indicate that consideration of the oscillatory conditions and the phase-lags in the multiple outputs of the network is important: The building and/or maintenance cost of the network can be reduced by introducing the oscillating condition, and when the phase-lag among the outputs is large, the transportation loss can also be reduced. We use stability analysis to reveal how the system exhibits various topologies depending on the parameter.
[Mh] Termos MeSH primário: Algoritmos
Transporte Biológico
[Mh] Termos MeSH secundário: Physarum
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1501
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140304
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0089231


  10 / 1017 MEDLINE  
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[PMID]:24109969
[Au] Autor:Mizukawa Y; Iwasaka M
[Ti] Título:Magnetic field effects on mitochondrion-activity-related optical properties in slime mold and bone forming cells.
[So] Source:Conf Proc IEEE Eng Med Biol Soc;2013:1442-5, 2013.
[Is] ISSN:1557-170X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In the present study, a cellular level response of Cyto-aa3 oxidation was investigated in real time under both time-varying and strong static magnetic fields of 5 T. Two kinds of cells, a slime mold, Physarum polycephalum, and bone forming cells, MC-3T3-E1, were used for the experiments. The oxidation level of the Cyto-aa3 was calculated by optical absorptions at 690 nm, 780 nm and 830 nm. The sample, fiber-optics and an additional optical fiber for light stimulation were set in a solenoidal coil or the bore of a 5-T superconducting magnet. The solenoidal coil for time-varying magnetic fields produced sinusoidal magnetic fields of 6 mT. The slime mold showed a periodic change in Cyto-aa3 oxidation, and the oxidation-reduction cycle of Cyto-aa3 was apparently changed when visible-light irradiated the slime mold. Similarly to the case with light, time-varying magnetic stimulations changed the oxidation-reduction cycle during and after the stimulation for 10 minutes. The same phenomena were observed in the MC-3T3-E1 cell assembly, although their cycle rhythm was comparatively random. Finally, magnetic field exposure of up to 5 T exhibited a distinct suppression of Cyto-aa3 oscillation in the bone forming cells. Exposure up to 5 T was repeated five times, and the change in Cyto-aa3 oxidation reproducibly occurred.
[Mh] Termos MeSH primário: Osso e Ossos/metabolismo
Complexo IV da Cadeia de Transporte de Elétrons/química
Campos Magnéticos
Mitocôndrias/fisiologia
Óptica e Fotônica/instrumentação
Physarum/metabolismo
[Mh] Termos MeSH secundário: Células 3T3
Animais
Luz
Camundongos
Óptica e Fotônica/métodos
Oxigênio/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
EC 1.9.3.1 (Electron Transport Complex IV); S88TT14065 (Oxygen)
[Em] Mês de entrada:1508
[Cu] Atualização por classe:131010
[Lr] Data última revisão:
131010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131011
[St] Status:MEDLINE
[do] DOI:10.1109/EMBC.2013.6609782



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