Base de dados : MEDLINE
Pesquisa : B01.050.050.199 [Categoria DeCS]
Referências encontradas : 9324 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 933 ir para página                         

  1 / 9324 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29216000
[Au] Autor:Babenko VN; Bragin AO; Spitsina AM; Chadaeva IV; Galieva ER; Orlova GV; Medvedeva IV; Orlov YL
[Ad] Endereço:, www.bionet.nsc.ru.
[Ti] Título:Analysis of differential gene expression by RNA-seq data in brain areas of laboratory animal.
[So] Source:J Integr Bioinform;13(4):1-15, 2016 Oct 01.
[Is] ISSN:1613-4516
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Computer analysis of gene expression in the nervous system plays a fundamental role in biology, genetics, and neurosciences. We studied molecular and genetic mechanisms of enhanced aggressiveness in comparison with tolerant behaviour using experimental animal models developed at the Institute of Cytology and Genetics SB RAS. Grey rats (Rattus norvegicus) have been subjected to selection during several generations in two directions - friendly, tolerant behaviour towards man (tame grey rats) and increased aggressive behaviour. We used samples from hypothalamus, mesencephalic tegmentum and periaqueductum grey matter from brain areas of grey rats genetically selected by behaviour in many generations. The set of computer tools and data processing pipelines helped to find genes and gene regulation patterns related to behaviour patterns. RNAprofiling experiments revealed the lists of differentially expressed genes in the contrast samples as well as differentially spliced isoforms. The gene ontology categories of protein transport, phosphoproteins, and nucleotide binding are presented together with categories of transmission of nerve impulses and neuron development were identified. Differential alternative splicing events found in the brain areas studied are statistically significant. We discuss role of alternative splicing events for neurospecific genes in behaviour patterns as well as extension of brain transcriptomics profiling.
[Mh] Termos MeSH primário: Animais de Laboratório
Encéfalo/metabolismo
Regulação da Expressão Gênica
RNA/metabolismo
Análise de Sequência de RNA
[Mh] Termos MeSH secundário: Animais
Ratos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
63231-63-0 (RNA)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171208
[St] Status:MEDLINE


  2 / 9324 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:27779465
[Au] Autor:Bartlett DH; Silk SB
[Ad] Endereço:Office of Laboratory Animal Welfare, National Institutes of Health , Bethesda, Maryland.
[Ti] Título:Office of Laboratory Animal Welfare Comments.
[So] Source:Zebrafish;13(6):563-564, 2016 12.
[Is] ISSN:1557-8542
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In support of the new column in Zebrafish dedicated to initiating conversations about animal care policies and compliance for the zebrafish, the Office of Laboratory Animal Welfare (OLAW) has offered the following clarification and guidance.
[Mh] Termos MeSH primário: Bem-Estar do Animal/normas
Aquicultura/normas
Peixe-Zebra
[Mh] Termos MeSH secundário: Animais
Animais de Laboratório
Guias como Assunto
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161026
[St] Status:MEDLINE


  3 / 9324 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29240762
[Au] Autor:Bert B; Dörendahl A; Leich N; Vietze J; Steinfath M; Chmielewska J; Hensel A; Grune B; Schönfelder G
[Ad] Endereço:German Federal Institute for Risk Assessment (BfR), German Centre for the Protection of Laboratory Animals (Bf3R), Berlin, Germany.
[Ti] Título:Rethinking 3R strategies: Digging deeper into AnimalTestInfo promotes transparency in in vivo biomedical research.
[So] Source:PLoS Biol;15(12):e2003217, 2017 Dec.
[Is] ISSN:1545-7885
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In the European Union (EU), animal welfare is seen as a matter of great importance. However, with respect to animal experimentation, European citizens feel quite uninformed. The European Directive 2010/63/EU for the protection of laboratory animals aims for greater transparency and requires that a comprehensible, nontechnical summary (NTS) of each authorised research project involving animals is published by the respective Member State. However, the NTSs remain sleeping beauties if their contents are not easily and systematically accessible. The German web-based NTS database AnimalTestInfo is a unique channel for scientists to communicate their work, and provides the opportunity for large-scale analyses of planned animal studies to inform researchers and the public. For an in-depth meta-analysis, we classified the duly completed NTSs submitted to AnimalTestInfo in 2014 and 2015 according to the International Classification of Diseases and Related Health Problems (ICD) system. Indexing the NTSs with ICD codes provided a fine-grained overview of the prospective uses of experimental animals. Using this approach, transparency, especially for highly controversial animal research involving, for example, nonhuman primates, is fostered, as it enables pinpointing the envisaged beneficiary down to the level of the addressed disease. Moreover, research areas with many planned projects involving animals can be specified in detail. The development of 3R (replacement, reduction, and refinement) measures in these research areas may be most efficient, as a large number of experimental animals would benefit from it. Indexing NTSs with ICD codes can support governments and funding agencies in advancing target-oriented funding of 3R research. Data drawn from NTSs can provide a basis for the development, validation, and implementation of directed 3R strategies as well as guidance for rethinking the role of animal research models.
[Mh] Termos MeSH primário: Experimentação Animal
Bem-Estar do Animal
Pesquisa Biomédica
Projetos de Pesquisa/legislação & jurisprudência
Projetos de Pesquisa/normas
[Mh] Termos MeSH secundário: Experimentação Animal/ética
Experimentação Animal/legislação & jurisprudência
Experimentação Animal/normas
Experimentação Animal/estatística & dados numéricos
Bem-Estar do Animal/ética
Bem-Estar do Animal/legislação & jurisprudência
Bem-Estar do Animal/normas
Bem-Estar do Animal/estatística & dados numéricos
Animais
Animais Domésticos
Animais de Laboratório
Animais Selvagens
Bioética
Pesquisa Biomédica/ética
Pesquisa Biomédica/legislação & jurisprudência
Pesquisa Biomédica/normas
Pesquisa Biomédica/estatística & dados numéricos
Alemanha/epidemiologia
Seres Humanos
Estudos Prospectivos
Projetos de Pesquisa/estatística & dados numéricos
Pesquisadores/ética
Pesquisadores/legislação & jurisprudência
Pesquisadores/normas
Pesquisadores/estatística & dados numéricos
[Pt] Tipo de publicação:JOURNAL ARTICLE; META-ANALYSIS
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171226
[Lr] Data última revisão:
171226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171215
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pbio.2003217


  4 / 9324 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29088279
[Au] Autor:Schmidt K; Butt J; Mauter P; Vogel K; Erles-Kemna A; Pawlita M; Nicklas W
[Ad] Endereço:Microbiological Diagnostics, Center for Preclinical Research, German Cancer Research Center, Heidelberg, Germany.
[Ti] Título:Development of a multiplex serological assay reveals a worldwide distribution of murine astrovirus infections in laboratory mice.
[So] Source:PLoS One;12(10):e0187174, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Laboratory mice play a tremendous role in biomedical research in studies on immunology, infection, cancer and therapy. In the course of standardization of mice used in animal experiments, health monitoring constitutes an important instrument towards microbiological standardization. Infections with murine astroviruses (MuAstV) were only recently discovered and are, therefore, still relatively unknown in laboratory animal science. In rodent health monitoring viral infections within a population are commonly assessed in terms of specific antibodies by serological testing, as active infection and excretion of virus is often temporary and can easily be missed. So far only ongoing infections with astroviruses can be detected by PCR. The objective of this work was the development of a sensitive and specific MuAstV multiplex serological assay with a high-throughput capability to be used in routine testing of laboratory mice. Four different MuAstV proteins were recombinantly expressed and used as antigens. The best reacting antigen, the capsid spike protein VP27, was selected and tested with a panel of 400 sera of mice from units with a known MuAstV status. Assay sensitivity and specificity resulted in 98.5% and 100%, respectively, compared to RT-PCR results. Eventually this assay was used to test 5529 serum samples in total, during routine diagnostics at the German Cancer Research Center (DKFZ) in Heidelberg between 2015 and 2017. High sero-prevalence rates of up to 98% were detected in units with open cages indicating that the virus is highly infectious and circulates within these populations virtually infecting all animals regardless of the mouse strain. In addition, data collected from 312 mice purchased from commercial breeders and from 661 mice from 58 research institutes in 15 countries worldwide allowed the conclusion that MuAstV is widespread in contemporary laboratory mouse populations.
[Mh] Termos MeSH primário: Animais de Laboratório/virologia
Infecções por Astroviridae/veterinária
Astroviridae
Camundongos/virologia
Reação em Cadeia da Polimerase Multiplex
Testes Sorológicos/métodos
[Mh] Termos MeSH secundário: Animais
Anticorpos Antivirais/imunologia
Astroviridae/imunologia
Infecções por Astroviridae/epidemiologia
Infecções por Astroviridae/imunologia
Infecções por Astroviridae/virologia
Reação em Cadeia da Polimerase Multiplex/métodos
Reação em Cadeia da Polimerase Multiplex/veterinária
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Sensibilidade e Especificidade
Estudos Soroepidemiológicos
Testes Sorológicos/veterinária
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Viral)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171101
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0187174


  5 / 9324 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28973392
[Au] Autor:Chen YC; Greenbaum J; Shen H; Deng HW
[Ad] Endereço:Department of Endocrinology and Metabolism, The Third Affiliated Hospital of Southern Medical University, Guangzhou 510630, PR China.
[Ti] Título:Association Between Gut Microbiota and Bone Health: Potential Mechanisms and Prospective.
[So] Source:J Clin Endocrinol Metab;102(10):3635-3646, 2017 Oct 01.
[Is] ISSN:1945-7197
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Context: It has been well established that the human gut microbiome plays a critical role in the regulation of important biological processes and the mechanisms underlying numerous complex diseases. Although researchers have only recently begun to study the relationship between the gut microbiota and bone metabolism, early efforts have provided increased evidence to suggest an important association. Evidence Acquisition: In this study, we attempt to comprehensively summarize the relationship between the gut microbiota and bone metabolism by detailing the regulatory effects of the microbiome on various biological processes, including nutrient absorption and the intestinal mucosal barrier, immune system functionality, the gut-brain axis, and excretion of functional byproducts. In this review, we incorporate evidence from various types of studies, including observational, in vitro and in vivo animal experiments, as well as small efficacy clinic trails. Evidence Synthesis: We review the various potential mechanisms of influence for the gut microbiota on the regulation of bone metabolism and discuss the importance of further examining the potential effects of the gut microbiota on the risk of osteoporosis in humans. Furthermore, we outline some useful tools/approaches for metagenomics research and present some prominent examples of metagenomics association studies in humans. Conclusion: Current research efforts, although limited, clearly indicate that the gut microbiota may be implicated in bone metabolism, and therefore, further exploration of this relationship is a promising area of focus in bone health and osteoporosis research. Although most existing studies investigate this relationship using animal models, human studies are both needed and on the horizon.
[Mh] Termos MeSH primário: Osso e Ossos/metabolismo
Microbioma Gastrointestinal/fisiologia
[Mh] Termos MeSH secundário: Animais
Animais de Laboratório
Antibacterianos/farmacologia
Reabsorção Óssea/microbiologia
Osso e Ossos/efeitos dos fármacos
Vida Livre de Germes
Saúde
Seres Humanos
Prebióticos
Probióticos/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Prebiotics)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:171004
[St] Status:MEDLINE
[do] DOI:10.1210/jc.2017-00513


  6 / 9324 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28880300
[Au] Autor:Moher D; Shamseer L; Cobey KD; Lalu MM; Galipeau J; Avey MT; Ahmadzai N; Alabousi M; Barbeau P; Beck A; Daniel R; Frank R; Ghannad M; Hamel C; Hersi M; Hutton B; Isupov I; McGrath TA; McInnes MDF; Page MJ; Pratt M; Pussegoda K; Shea B; Srivastava A; Stevens A; Thavorn K; van Katwyk S; Ward R; Wolfe D; Yazdi F; Yu AM; Ziai H
[Ad] Endereço:Ottawa Hospital Research Institute, Ontario, Canada, and part of the Study Reporting in Predatory Journals Group.
[Ti] Título:Stop this waste of people, animals and money.
[So] Source:Nature;549(7670):23-25, 2017 09 06.
[Is] ISSN:1476-4687
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Animais de Laboratório
Pesquisa Biomédica/estatística & dados numéricos
Países Desenvolvidos/estatística & dados numéricos
Países em Desenvolvimento/estatística & dados numéricos
Publicações Periódicas como Assunto/ética
Editoração/ética
Pesquisadores/estatística & dados numéricos
[Mh] Termos MeSH secundário: Animais
Autoria
Aprendizagem da Esquiva
Pesquisa Biomédica/normas
Ensaios Clínicos como Assunto/utilização
Países Desenvolvidos/economia
Países em Desenvolvimento/economia
Guias como Assunto
Seres Humanos
Internacionalidade
National Institutes of Health (U.S.)/economia
Publicação de Acesso Aberto/economia
Publicação de Acesso Aberto/provisão & distribuição
Publicação de Acesso Aberto/utilização
Publicações Periódicas como Assunto/normas
Editoração/normas
Editoração/provisão & distribuição
Pesquisadores/educação
Pesquisadores/ética
Pesquisadores/normas
Apoio à Pesquisa como Assunto/normas
Apoio à Pesquisa como Assunto/estatística & dados numéricos
Literatura de Revisão como Assunto
Estados Unidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170908
[St] Status:MEDLINE
[do] DOI:10.1038/549023a


  7 / 9324 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28880297
[Au] Autor:Marris E
[Ti] Título:How labs are coping with Hurricane Harvey's devastating floods.
[So] Source:Nature;549(7670):13-14, 2017 08 31.
[Is] ISSN:1476-4687
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Adaptação Psicológica
Tempestades Ciclônicas
Inundações
Laboratórios/organização & administração
Pesquisadores/psicologia
Pesquisa/organização & administração
[Mh] Termos MeSH secundário: Animais
Animais de Laboratório
Tempestades Ciclônicas/história
Equipamentos e Provisões
Inundações/história
História do Século XXI
Chuvas
Socorro em Desastres
Pesquisa/instrumentação
Mídias Sociais
Astronave
Telescópios
Texas
[Pt] Tipo de publicação:HISTORICAL ARTICLE; JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170908
[St] Status:MEDLINE
[do] DOI:10.1038/nature.2017.22542


  8 / 9324 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28821637
[Au] Autor:Hansen BC; Gografe S; Pritt S; Jen KC; McWhirter CA; Barman SM; Comuzzie A; Greene M; McNulty JA; Michele DE; Moaddab N; Nelson RJ; Norris K; Uray KD; Banks R; Westlund KN; Yates BJ; Silverman J; Hansen KD; Redman B
[Ad] Endereço:Department of Internal Medicine and Pediatrics, University of South Florida, Tampa, Florida, USA; bhansen@health.usf.edu.
[Ti] Título:Ensuring due process in the IACUC and animal welfare setting: considerations in developing noncompliance policies and procedures for institutional animal care and use committees and institutional officials.
[So] Source:FASEB J;31(10):4216-4225, 2017 Oct.
[Is] ISSN:1530-6860
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Every institution that is involved in research with animals is expected to have in place policies and procedures for the management of allegations of noncompliance with the Animal Welfare Act and the U.S. Public Health Service Policy on the Humane Care and Use of Laboratory Animals. We present here a model set of recommendations for institutional animal care and use committees and institutional officials to ensure appropriate consideration of allegations of noncompliance with federal Animal Welfare Act regulations that carry a significant risk or specific threat to animal welfare. This guidance has 3 overarching aims: ) protecting the welfare of research animals; ) according fair treatment and due process to an individual accused of noncompliance; and ) ensuring compliance with federal regulations. Through this guidance, the present work seeks to advance the cause of scientific integrity, animal welfare, and the public trust while recognizing and supporting the critical importance of animal research for the betterment of the health of both humans and animals.-Hansen, B. C., Gografe, S., Pritt, S., Jen, K.-L. C., McWhirter, C. A., Barman, S. M., Comuzzie, A., Greene, M., McNulty, J. A., Michele, D. E., Moaddab, N., Nelson, R. J., Norris, K., Uray, K. D., Banks, R., Westlund, K. N., Yates, B. J., Silverman, J., Hansen, K. D., Redman, B. Ensuring due process in the IACUC and animal welfare setting: considerations in developing noncompliance policies and procedures for institutional animal care and use committees and institutional officials.
[Mh] Termos MeSH primário: Comitês de Cuidado Animal
Experimentação Animal
Bem-Estar do Animal
Animais de Laboratório
Direitos Civis
[Mh] Termos MeSH secundário: Experimentação Animal/normas
Bem-Estar do Animal/legislação & jurisprudência
Animais
DNA/metabolismo
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
9007-49-2 (DNA)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170820
[St] Status:MEDLINE
[do] DOI:10.1096/fj.201601250R


  9 / 9324 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28817724
[Au] Autor:Arriero E; Wanelik KM; Birtles RJ; Bradley JE; Jackson JA; Paterson S; Begon M
[Ad] Endereço:School of Life Sciences, University of Nottingham, Nottingham, United Kingdom.
[Ti] Título:From the animal house to the field: Are there consistent individual differences in immunological profile in wild populations of field voles (Microtus agrestis)?
[So] Source:PLoS One;12(8):e0183450, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Inbred mouse strains, living in simple laboratory environments far removed from nature, have been shown to vary consistently in their immune response. However, wildlife populations are typically outbreeding and face a multiplicity of challenges, parasitological and otherwise. In this study we seek evidence of consistent difference in immunological profile amongst individuals in the wild. We apply a novel method in this context, using longitudinal (repeated capture) data from natural populations of field voles, Microtus agrestis, on a range of life history and infection metrics, and on gene expression levels. We focus on three immune genes, IFN-γ, Gata3, and IL-10, representing respectively the Th1, Th2 and regulatory elements of the immune response. Our results show that there was clear evidence of consistent differences between individuals in their typical level of expression of at least one immune gene, and at most all three immune genes, after other measured sources of variation had been taken into account. Furthermore, individuals that responded to changing circumstances by increasing expression levels of Gata3 had a correlated increase in expression levels of IFN-γ. Our work stresses the importance of acknowledging immunological variation amongst individuals in studies of parasitological and infectious disease risk in wildlife populations.
[Mh] Termos MeSH primário: Animais de Laboratório
Animais Selvagens
Arvicolinae/imunologia
[Mh] Termos MeSH secundário: Animais
Arvicolinae/genética
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170818
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183450


  10 / 9324 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
[PMID]:28816055
[Au] Autor:Sneddon LU
[Ad] Endereço:University of Liverpool, Liverpool, UK.
[Ti] Título:Pain in laboratory animals: A possible confounding factor?
[So] Source:Altern Lab Anim;45(3):161-164, 2017 Jul.
[Is] ISSN:0261-1929
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Reducing pain in animals is an ethical and sometimes legal requirement, but how do we assess pain and does it confound data collection?
[Mh] Termos MeSH primário: Experimentação Animal/ética
Animais de Laboratório
Dor/prevenção & controle
[Mh] Termos MeSH secundário: Animais
Medição da Dor
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171003
[Lr] Data última revisão:
171003
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170818
[St] Status:MEDLINE



página 1 de 933 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde