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Pesquisa : B01.050.150.900.090.608.630.510 [Categoria DeCS]
Referências encontradas : 424 [refinar]
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[PMID]:27023006
[Au] Autor:Lavrov I; Fox L; Shen J; Han Y; Cheng J
[Ad] Endereço:Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Russia.
[Ti] Título:Gap Junctions Contribute to the Regulation of Walking-Like Activity in the Adult Mudpuppy (Necturus Maculatus).
[So] Source:PLoS One;11(3):e0152650, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Although gap junctions are widely expressed in the developing central nervous system, the role of electrical coupling of neurons and glial cells via gap junctions in the spinal cord in adults is largely unknown. We investigated whether gap junctions are expressed in the mature spinal cord of the mudpuppy and tested the effects of applying gap junction blocker on the walking-like activity induced by NMDA or glutamate in an in vitro mudpuppy preparation. We found that glial and neural cells in the mudpuppy spinal cord expressed different types of connexins that include connexin 32 (Cx32), connexin 36 (Cx36), connexin 37 (Cx37), and connexin 43 (Cx43). Application of a battery of gap junction blockers from three different structural classes (carbenexolone, flufenamic acid, and long chain alcohols) substantially and consistently altered the locomotor-like activity in a dose-dependent manner. In contrast, these blockers did not significantly change the amplitude of the dorsal root reflex, indicating that gap junction blockers did not inhibit neuronal excitability nonselectively in the spinal cord. Taken together, these results suggest that gap junctions play a significant modulatory role in the spinal neural networks responsible for the generation of walking-like activity in the adult mudpuppy.
[Mh] Termos MeSH primário: Junções Comunicantes/metabolismo
Rede Nervosa/fisiologia
Coluna Vertebral/metabolismo
Caminhada/fisiologia
[Mh] Termos MeSH secundário: Proteínas de Anfíbios/biossíntese
Animais
Conexinas/biossíntese
Ácido Glutâmico/metabolismo
N-Metilaspartato/metabolismo
Necturus
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Amphibian Proteins); 0 (Connexins); 3KX376GY7L (Glutamic Acid); 6384-92-5 (N-Methylaspartate)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:161019
[Lr] Data última revisão:
161019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160330
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0152650


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[PMID]:16371573
[Au] Autor:Zhang W; Delay RJ
[Ad] Endereço:104 Marsh Life Science Building, Department of Biology, University of Vermont, Burlington, VT 05405, USA.
[Ti] Título:Pulse stimulation with odors or IBMX/forskolin potentiates responses in isolated olfactory neurons.
[So] Source:Chem Senses;31(3):197-206, 2006 Mar.
[Is] ISSN:0379-864X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Many odor responses are mediated by the adenosine 3',5'-cyclic monophosphate (cAMP) pathway in which the cAMP-gated current is amplified by Ca2+-dependent Cl- current. In olfactory neurons, prolonged exposure to odors decreases the odor response and is an adaptive effect. Several studies suggest that odor adaptation is linked to elevated intracellular Ca2+. In the present study, using the perforated configuration of the patch clamp technique, we found that repetitive odor stimulation elicits a potentiation of the subsequent responses in olfactory neurons. This potentiation is mimicked by stimulating the cAMP pathway and does not appear to be related to phosphorylation of ion channels since protein kinase inhibitors could not block it. Our data suggest that local increases in [Ca2+]i via activation of the cAMP pathway mediate the pulse-elicited potentiation. In the first odor application, entry of Ca2+ through cyclic nucleotide-gated channels appears to be buffered. Repetitive stimulation allows local increases in [Ca2+]i, recruiting more Ca2+-dependent Cl- channels with each subsequent odor pulse.
[Mh] Termos MeSH primário: 1-Metil-3-Isobutilxantina/farmacologia
Cálcio/farmacologia
Cloretos/farmacologia
Colforsina/farmacologia
Neurônios Receptores Olfatórios/fisiologia
Olfato/fisiologia
[Mh] Termos MeSH secundário: Animais
Canais de Cálcio/fisiologia
Separação Celular
Células Cultivadas
Canais de Cloreto/fisiologia
Sinergismo Farmacológico
Eletrofisiologia
Feminino
Masculino
Modelos Biológicos
Necturus
Neurônios Receptores Olfatórios/efeitos dos fármacos
Técnicas de Patch-Clamp
Inibidores de Proteínas Quinases/farmacologia
Olfato/efeitos dos fármacos
Estimulação Química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Calcium Channels); 0 (Chloride Channels); 0 (Chlorides); 0 (Protein Kinase Inhibitors); 1F7A44V6OU (Colforsin); SY7Q814VUP (Calcium); TBT296U68M (1-Methyl-3-isobutylxanthine)
[Em] Mês de entrada:0609
[Cu] Atualização por classe:161019
[Lr] Data última revisão:
161019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:051224
[St] Status:MEDLINE


  3 / 424 MEDLINE  
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[PMID]:14980383
[Au] Autor:Barstow KL; Locknar SA; Merriam LA; Parsons RL
[Ad] Endereço:Department of Anatomy and Neurobiology, University of Vermont College of Medicine, 89 Beaumont Avenue, Burlington, VT 05405, USA.
[Ti] Título:The modulation of action potential generation by calcium-induced calcium release is enhanced by mitochondrial inhibitors in mudpuppy parasympathetic neurons.
[So] Source:Neuroscience;124(2):327-39, 2004.
[Is] ISSN:0306-4522
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Previously, we demonstrated that outward currents activated by calcium-induced calcium release (CICR) opposed depolarization-induced action potential (AP) generation in dissociated mudpuppy parasympathetic neurons [J Neurophysiol 88 (2002) 1119]. In the present study, we tested whether AP generation by depolarizing current ramps could be altered by dissipating the mitochondrial membrane potential and thus interrupting mitochondrial Ca2+ buffering. Exposure to the protonophore carbonyl cyanide m-chlorophenylhydrazone (CCCP; 2 microM) alone or in combination with the mitochondrial ATP synthase inhibitor oligomycin (8 microg/ml), increased the latency to AP generation. Exposure to the electron transport chain inhibitor rotenone (10 microM) alone or in combination with oligomycin (8 microg/ml) similarly increased the latency to AP generation. CCCP and oligomycin or rotenone and oligomycin treatment caused rhodamine 123 loss from mitochondria within a few minutes, confirming that the mitochondrial membrane potential was dissipated during drug exposure. Oligomycin alone had no effect on the latency to AP generation and did not cause loss of rhodamine 123 from mitochondria. The increase in latency induced by CCCP and oligomycin was similar when recordings were made with either the perforated patch or standard whole cell patch recording configuration. Exposure to the endoplasmic reticulum Ca-ATPase inhibitor thapsigargin (1 microM), decreased the latency to AP generation. In cells pretreated with thapsigargin to eliminate CICR, CCCP and oligomycin had no effect on AP latency. Pretreatment with iberiotoxin (IBX; 100 nM), an inhibitor of large conductance, calcium- and voltage-activated potassium channels, reduced the extent of the CCCP- and oligomycin-induced increase in latency to AP generation. These results indicate that treatment with CCCP or rotenone to dissipate the mitochondrial membrane potential, a condition which should minimize sequestration of Ca2+ by mitochondria, facilitated the Ca(2+)-induced Ca2+ release activation of IBX-sensitive and IBX-insensitive conductances that regulate AP generation.
[Mh] Termos MeSH primário: Potenciais de Ação/efeitos dos fármacos
Cálcio/metabolismo
Ácido Egtázico/análogos & derivados
Gânglios Parassimpáticos/citologia
Neurônios/efeitos dos fármacos
Oligomicinas/farmacologia
[Mh] Termos MeSH secundário: Potenciais de Ação/fisiologia
Compostos de Anilina/metabolismo
Animais
Carbonil Cianeto m-Clorofenil Hidrazona/farmacologia
Quelantes/farmacologia
Interações Medicamentosas
Ácido Egtázico/farmacologia
Inibidores Enzimáticos/farmacologia
Gânglios Parassimpáticos/efeitos dos fármacos
Iodobenzenos/farmacologia
Ionóforos/farmacologia
Potenciais da Membrana
Mitocôndrias/efeitos dos fármacos
Necturus
Neurônios/citologia
Técnicas de Cultura de Órgãos
Técnicas de Patch-Clamp/métodos
Tempo de Reação/efeitos dos fármacos
Rodamina 123/metabolismo
Rotenona/farmacologia
Desacopladores/farmacologia
Xantenos/metabolismo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.; RESEARCH SUPPORT, U.S. GOV'T, P.H.S.
[Nm] Nome de substância:
0 (Aniline Compounds); 0 (Chelating Agents); 0 (Enzyme Inhibitors); 0 (Iodobenzenes); 0 (Ionophores); 0 (Oligomycins); 0 (Uncoupling Agents); 0 (Xanthenes); 03L9OT429T (Rotenone); 1N3CZ14C5O (Rhodamine 123); 23D4W0B50Y (Fluo-3); 3K0C43POH0 (o-iodoxybenzoic acid); 526U7A2651 (Egtazic Acid); 555-60-2 (Carbonyl Cyanide m-Chlorophenyl Hydrazone); K22DDW77C0 (1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid); SY7Q814VUP (Calcium)
[Em] Mês de entrada:0406
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:040226
[St] Status:MEDLINE


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[PMID]:12887980
[Au] Autor:Bigiani A; Ghiaroni V; Fieni F
[Ad] Endereço:Dipartimento di Scienze Biomediche, Sezione di Fisiologia, Università di Modena e Reggio Emilia, via Campi 287, 41100 Modena, Italy. bigiani@unimore.it
[Ti] Título:Channels as taste receptors in vertebrates.
[So] Source:Prog Biophys Mol Biol;83(3):193-225, 2003 Nov.
[Is] ISSN:0079-6107
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Taste reception is fundamental for proper selection of food and beverages. Chemicals detected as taste stimuli by vertebrates include a large variety of substances, ranging from inorganic ions (e.g., Na(+), H(+)) to more complex molecules (e.g., sucrose, amino acids, alkaloids). Specialized epithelial cells, called taste receptor cells (TRCs), express specific membrane proteins that function as receptors for taste stimuli. Classical view of the early events in chemical detection was based on the assumption that taste substances bind to membrane receptors in TRCs without permeating the tissue. Although this model is still valid for some chemicals, such as sucrose, it does not hold for small ions, such as Na(+), that actually diffuse inside the taste tissue through ion channels. Electrophysiological, pharmacological, biochemical, and molecular biological studies have provided evidence that indeed TRCs use ion channels to reveal the presence of certain substances in foodstuff. In this review, we focus on the functional and molecular properties of ion channels that serve as receptors in taste transduction.
[Mh] Termos MeSH primário: Canais Iônicos/fisiologia
Células Receptoras Sensoriais/fisiologia
Papilas Gustativas/fisiologia
[Mh] Termos MeSH secundário: Animais
Anuros
Canais Epiteliais de Sódio
Seres Humanos
Potenciais da Membrana/fisiologia
Necturus
Canais de Potássio de Abertura Dependente da Tensão da Membrana/fisiologia
Transdução de Sinais/fisiologia
Canais de Sódio/fisiologia
Estimulação Química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Nm] Nome de substância:
0 (Epithelial Sodium Channels); 0 (Ion Channels); 0 (Potassium Channels, Voltage-Gated); 0 (Sodium Channels)
[Em] Mês de entrada:0405
[Cu] Atualização por classe:121115
[Lr] Data última revisão:
121115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:030731
[St] Status:MEDLINE


  5 / 424 MEDLINE  
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[PMID]:12789493
[Au] Autor:Yang SX; Ogmen H; Maguire G
[Ad] Endereço:School of Engineering, University of Guelph, Guelph, Ontario N1G 2W1, Canada. syang@uoguelph.ca
[Ti] Título:Neural computations in the tiger salamander and mudpuppy outer retinae and an analysis of GABA action from horizontal cells.
[So] Source:Biol Cybern;88(6):450-8, 2003 Jun.
[Is] ISSN:0340-1200
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:A neural network architecture based on the neural anatomy and function of retinal neurons in tiger salamander and mudpuppy retinae is proposed to study basic aspects of early visual information processing. The model predictions for the main response characteristics of retinal neurons are found to be in agreement with neurophysiological data, including the antagonistic role of horizontal cells in the outer plexiform layer. The examination of possible gamma-aminobutyric acid (GABA) action from horizontal cells suggests that GABA(A) alone, GABA(B) alone, or their weighted combination can generate the response characteristics observed in bipolar cells.
[Mh] Termos MeSH primário: Ambystoma/fisiologia
Modelos Neurológicos
Necturus/fisiologia
Rede Nervosa/fisiologia
Retina/citologia
Retina/fisiologia
Ácido gama-Aminobutírico/fisiologia
[Mh] Termos MeSH secundário: Ambystoma/anatomia & histologia
Animais
Necturus/anatomia & histologia
Redes Neurais (Computação)
Estimulação Luminosa/métodos
Células Fotorreceptoras/fisiologia
Células Ganglionares da Retina/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
56-12-2 (gamma-Aminobutyric Acid)
[Em] Mês de entrada:0402
[Cu] Atualização por classe:081121
[Lr] Data última revisão:
081121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:030606
[St] Status:MEDLINE


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[PMID]:12589907
[Au] Autor:Tresch MC; Saltiel P; d'Avella A; Bizzi E
[Ad] Endereço:Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
[Ti] Título:Coordination and localization in spinal motor systems.
[So] Source:Brain Res Brain Res Rev;40(1-3):66-79, 2002 Oct.
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:We review here experiments examining the hypothesis that vertebrate spinal motor systems produce movement through the flexible combination of a small number of units of motor output. Using a variety of preparations and techniques, these experiments provide evidence for such spinally generated units and for the localization of the networks responsible for producing them within different regions of the spinal cord. Such an organization might help to simplify the production of movement, reducing the degrees of freedom that need to be specified by providing a set of units involved in regulating features common to a range of behaviors.
[Mh] Termos MeSH primário: Neurônios Motores/fisiologia
Movimento/fisiologia
Medula Espinal/fisiologia
[Mh] Termos MeSH secundário: Animais
Anuros
Mamíferos
Necturus
Reflexo/fisiologia
Tartarugas
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, P.H.S.; REVIEW
[Em] Mês de entrada:0304
[Cu] Atualização por classe:071114
[Lr] Data última revisão:
071114
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:030219
[St] Status:MEDLINE


  7 / 424 MEDLINE  
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[PMID]:12110959
[Au] Autor:Cheng J; Jovanovic K; Aoyagi Y; Bennett DJ; Han Y; Stein RB
[Ad] Endereço:Department of Neurological Surgery, University of Louisville School of Medicine, Louisville, KY 40202, USA.
[Ti] Título:Differential distribution of interneurons in the neural networks that control walking in the mudpuppy (Necturus maculatus) spinal cord.
[So] Source:Exp Brain Res;145(2):190-8, 2002 Jul.
[Is] ISSN:0014-4819
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Locomotor behavior is believed to be produced by interneuronal networks that are intrinsically organized to generate the underlying complex spatiotemporal patterns. In order to study the temporal correlation between the firing of individual interneurons and the pattern of locomotion, we utilized the spinal cord-forelimb preparation from the mudpuppy, in which electrophysiological recordings of neuronal activity were achieved during walking-like movement of the forelimb induced by bath application of N-methyl- D-aspartate (NMDA). Intra- and extracellular recordings were made in the C2 and C3 segments of the spinal cord. These segments contain independent flexor and extensor centers for the forelimb movement about the elbow joint during walking. Among the 289 cells recorded in the intermediate gray matter (an area between the ventral and dorsal horns) of the C2 and C3 segments, approximately 40% of the cells fired rhythmically during "walking." The firing rates were 6.4+/-0.4 impulses/s (mean +/- SE). These rhythmically active cells were classified into four types based on their phase of activity during a normalized step cycle. About half the rhythmic cells fired in phase with either the flexor (F) or extensor (E) motoneurons. The rest fired in the transitions between the two phases (F-->E and E-->F). Longitudinal distributions of the four types of interneurons along the spinal cord were in agreement with observations that revealed distinct but overlapping flexor and extensor centers for walking. Some cells triggered short-latency responses in the elbow flexor or extensor muscles and may be last-order interneurons. These observations suggest that there is a differential distribution of phase-specific interneurons in the central pattern generator of the mudpuppy spinal cord for walking.
[Mh] Termos MeSH primário: Interneurônios/fisiologia
Rede Nervosa/fisiologia
Medula Espinal/fisiologia
Caminhada/fisiologia
[Mh] Termos MeSH secundário: Potenciais de Ação/fisiologia
Animais
Vértebras Cervicais/inervação
Vértebras Cervicais/fisiologia
Técnicas In Vitro
Interneurônios/classificação
Interneurônios/citologia
Necturus
Medula Espinal/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:0210
[Cu] Atualização por classe:141120
[Lr] Data última revisão:
141120
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:020712
[St] Status:MEDLINE


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[PMID]:12097737
[Au] Autor:Purkerson ML; Klahr S
[Ad] Endereço:Department of Medicine, Renal Division, Washington University School of Medicine, Box 8132, 660 S. Euclid Avenue, St. Louis, MO 63110-1093, USA. purkerm@msnotes.wustl.edu
[Ti] Título:Renal physiology between two wars: the contributions of Dr. Harvey Lester White.
[So] Source:Am J Nephrol;22(2-3):180-5, 2002 Jul.
[Is] ISSN:0250-8095
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Harvey Lester White (1896-1977) graduated from Washington University (St. Louis) School of Medicine and subsequently spent his entire professional career in the School's Department of Physiology. White's interest in the function of the kidney was evident early in his academic career when he pioneered research related to renal physiology. His first papers dealt with studies of renal tubular function (1923). He performed what appear to represent the first micropuncture studies in the kidney of Necturus maculosus (1926) and thus confirmed the observations of Wearn and Richards regarding glomerular filtration. Through these studies, he was the first to show that glucose reabsorption occurs in the proximal tubules of Necturus. It also appears that White et al. were the first to demonstrate that volume expansion increases bicarbonate excretion in dogs (1926). He studied the influence of posture on renal "activity" in man (1926). Intracapsular pressure determinations in Necturus kidney (1928) were done using micropuncture techniques. White and co-workers presented (1933) a comparison of clearances of creatinine and various sugars confirming the work of Jolliffe, Shannon and Smith (1932) who had proposed the clearances of non-metabolizable sugars to be a measure of glomerular filtration. Between 1932 and 1936, in an effort to better understand the process of glomerular filtration, he began a series of studies on streaming potentials, surface conductance, electro-endosmosis and other related topics. In 1937, White began work on yet another aspect of renal physiology--endocrine influences on renal function. He would immerse himself in these investigations throughout the remainder of his scientific career. We hope that this account will reveal at least a small dimension of the man and his contributions to renal physiology.
[Mh] Termos MeSH primário: Nefrologia/história
[Mh] Termos MeSH secundário: Animais
Cães
História do Século XX
Rim/fisiologia
Glomérulos Renais/fisiologia
Necturus
Estados Unidos
[Pt] Tipo de publicação:BIOGRAPHY; HISTORICAL ARTICLE; JOURNAL ARTICLE; PORTRAITS
[Ps] Nome de pessoa como assunto:White HL
[Em] Mês de entrada:0210
[Cu] Atualização por classe:171101
[Lr] Data última revisão:
171101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:020705
[St] Status:MEDLINE


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[PMID]:12037215
[Au] Autor:Ogura T; Margolskee RF; Kinnamon SC
[Ad] Endereço:Department of Anatomy and Neurobiology, Colorado State University, Fort Collins, Colorado 80523, USA. Tatsuya.Ogura@colostate.edu
[Ti] Título:Taste receptor cell responses to the bitter stimulus denatonium involve Ca2+ influx via store-operated channels.
[So] Source:J Neurophysiol;87(6):3152-5, 2002 Jun.
[Is] ISSN:0022-3077
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Previous studies in rat and mouse have shown that brief exposure to the bitter stimulus denatonium induces an increase in [Ca2+]i due to Ca2+ release from intracellular Ca2+ stores, rather than Ca2+ influx. We report here that prolonged exposure to denatonium induces sustained increases in [Ca2+]i that are dependent on Ca2+ influx. Similar results were obtained from taste cells of the mudpuppy, Necturus maculosus, as well as green fluorescent protein (GFP) tagged gustducin-expressing taste cells of transgenic mice. In a subset of mudpuppy taste cells, prolonged exposure to denatonium induced oscillatory Ca2+ responses. Depletion of Ca2+ stores by thapsigargin also induced Ca2+ influx, suggesting that Ca2+ store-operated channels (SOCs) are present in both mudpuppy taste cells and gustducin-expressing taste cells of mouse. Further, treatment with thapsigargin prevented subsequent responses to denatonium, suggesting that the SOCs were the source of the Ca2+ influx. These data suggest that SOCs may contribute to bitter taste transduction and to regulation of Ca2+ homeostasis in taste cells.
[Mh] Termos MeSH primário: Canais de Cálcio/fisiologia
Cálcio/metabolismo
Papilas Gustativas/fisiologia
Paladar/fisiologia
[Mh] Termos MeSH secundário: Animais
Cálcio/farmacologia
Inibidores Enzimáticos/farmacologia
Expressão Gênica/fisiologia
Camundongos
Camundongos Transgênicos
Necturus
Compostos de Amônio Quaternário
Estimulação Química
Tapsigargina/farmacologia
Transducina/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, P.H.S.
[Nm] Nome de substância:
0 (Calcium Channels); 0 (Enzyme Inhibitors); 0 (Quaternary Ammonium Compounds); 0W5171805N (denatonium chloride); 147979-21-3 (gustducin); 67526-95-8 (Thapsigargin); EC 3.6.5.1 (Transducin); SY7Q814VUP (Calcium)
[Em] Mês de entrada:0208
[Cu] Atualização por classe:161124
[Lr] Data última revisão:
161124
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:020531
[St] Status:MEDLINE


  10 / 424 MEDLINE  
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[PMID]:12037167
[Au] Autor:Ogura T
[Ad] Endereço:Department of Anatomy and Neurobiology, Colorado State University, Fort Collins 80523, USA. togura@lamar.colostate.edu
[Ti] Título:Acetylcholine increases intracellular Ca2+ in taste cells via activation of muscarinic receptors.
[So] Source:J Neurophysiol;87(6):2643-9, 2002 Jun.
[Is] ISSN:0022-3077
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Previous studies suggest that acetylcholine (ACh) is a transmitter released from taste cells as well as a transmitter in cholinergic efferent neurons innervating taste buds. However, the physiological effects on taste cells have not been established. I examined effects of ACh on taste-receptor cells by monitoring [Ca2+]i. ACh increased [Ca2+]i in both rat and mudpuppy taste cells. Atropine blocked the ACh response, but D-tubocurarine did not. U73122, a phospholipase C inhibitor, and thapsigargin, a Ca2+-ATPase inhibitor that depletes intracellular Ca2+ stores, blocked the ACh response. These results suggest that ACh binds to M1/M3/M5-like subtypes of muscarinic ACh receptors, causing an increase in inositol 1,4,5-trisphosphate and subsequent release of Ca2+ from the intracellular stores. A long incubation with ACh induced a transient response followed by a sustained phase of [Ca2+]i increase. In Ca2+-free solution, the sustained phases disappeared, suggesting that Ca2+ influx is involved in the sustained phase. Depletion of Ca2+ stores by thapsigargin alone induced Ca2+ influx. These findings suggest that Ca2+ store-operated channels may be present in taste cells and that they may participate in the sustained phase of [Ca2+]i increase. Immunocytochemical experiments indicated that the M1 subtype of muscarinic receptors is present in both rat and mudpuppy taste cells.
[Mh] Termos MeSH primário: Acetilcolina/farmacologia
Cálcio/metabolismo
Receptores Muscarínicos/metabolismo
Papilas Gustativas/efeitos dos fármacos
Papilas Gustativas/metabolismo
[Mh] Termos MeSH secundário: Animais
Anticorpos
Necturus
Ratos
Ratos Sprague-Dawley
Receptor Muscarínico M1
Receptor Muscarínico M2
Receptor Muscarínico M5
Receptores Muscarínicos/análise
Receptores Muscarínicos/imunologia
Papilas Gustativas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, P.H.S.
[Nm] Nome de substância:
0 (Antibodies); 0 (Receptor, Muscarinic M1); 0 (Receptor, Muscarinic M2); 0 (Receptor, Muscarinic M5); 0 (Receptors, Muscarinic); N9YNS0M02X (Acetylcholine); SY7Q814VUP (Calcium)
[Em] Mês de entrada:0208
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:020531
[St] Status:MEDLINE



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