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Pesquisa : B01.050.150.900.090.608.700.500 [Categoria DeCS]
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  1 / 16 MEDLINE  
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[PMID]:26912042
[Au] Autor:Rothermel BB; Miller DL; Travis ER; Gonynor McGuire JL; Jensen JB; Yabsley MJ
[Ad] Endereço:Archbold Biological Station, Venus, Florida 33960, USA.
[Ti] Título:Disease dynamics of red-spotted newts and their anuran prey in a montane pond community.
[So] Source:Dis Aquat Organ;118(2):113-27, 2016 Feb 25.
[Is] ISSN:0177-5103
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Long-term monitoring of amphibians is needed to clarify population-level effects of ranaviruses (Rv) and the fungal pathogen Batrachochytrium dendrobatidis (Bd). We investigated disease dynamics of co-occurring amphibian species and potential demographic consequences of Rv and Bd infections at a montane site in the Southern Appalachians, Georgia, USA. Our 3-yr study was unique in combining disease surveillance with intensive population monitoring at a site where both pathogens are present. We detected sub-clinical Bd infections in larval and adult red-spotted newts Notophthalmus viridescens viridescens, but found no effect of Bd on body condition of adult newts. Bd infections also occurred in larvae of 5 anuran species that bred in our fishless study pond, and we detected co-infections with Bd and Rv in adult newts and larval green frogs Lithobates clamitans. However, all mortality and clinical signs in adult newts and larval anurans were most consistent with ranaviral disease, including a die-off of larval wood frogs Lithobates sylvaticus in small fish ponds located near our main study pond. During 2 yr of drift fence monitoring, we documented high juvenile production in newts, green frogs and American bullfrogs L. catesbeianus, but saw no evidence of juvenile recruitment in wood frogs. Larvae of this susceptible species may have suffered high mortality in the presence of both Rv and predators. Our findings were generally consistent with results of Rv-exposure experiments and support the purported role of red-spotted newts, green frogs, and American bullfrogs as common reservoirs for Bd and/or Rv in permanent and semi-permanent wetlands.
[Mh] Termos MeSH primário: Notophthalmus/microbiologia
Notophthalmus/virologia
Tanques
Comportamento Predatório
Rana clamitans/microbiologia
Rana clamitans/virologia
[Mh] Termos MeSH secundário: Animais
Quitridiomicetos/imunologia
Infecções por Vírus de DNA/epidemiologia
Infecções por Vírus de DNA/veterinária
Infecções por Vírus de DNA/virologia
Georgia
Larva/microbiologia
Larva/virologia
Micoses/microbiologia
Micoses/transmissão
Micoses/veterinária
Rana clamitans/fisiologia
Ranavirus/isolamento & purificação
Estações do Ano
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Em] Mês de entrada:1605
[Cu] Atualização por classe:160225
[Lr] Data última revisão:
160225
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160226
[St] Status:MEDLINE
[do] DOI:10.3354/dao02965


  2 / 16 MEDLINE  
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[PMID]:22892678
[Au] Autor:Kaufmann R; Cremer C; Gall JG
[Ad] Endereço:Kirchhoff Institute for Physics (KIP), University of Heidelberg, Heidelberg, Germany.
[Ti] Título:Superresolution imaging of transcription units on newt lampbrush chromosomes.
[So] Source:Chromosome Res;20(8):1009-15, 2012 Dec.
[Is] ISSN:1573-6849
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:We have examined transcription loops on lampbrush chromosomes of the newt Notophthalmus by superresolution microscopy. Because of the favorable, essentially two-dimensional morphology of these loops, an average optical resolution in the x-y plane of about 50 nm was achieved. We analyzed the distribution of the multifunctional RNA-binding protein CELF1 on specific loops. CELF1 distribution is consistent with a model in which individual transcripts are tightly folded and hence closely packed against the loop axis.
[Mh] Termos MeSH primário: Cromossomos/genética
Microscopia de Fluorescência/métodos
Notophthalmus/genética
Transcrição Genética
[Mh] Termos MeSH secundário: Animais
Cromossomos/ultraestrutura
Feminino
RNA Polimerase II/genética
RNA Polimerase II/metabolismo
Proteínas de Ligação a RNA/genética
Proteínas de Ligação a RNA/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA-Binding Proteins); EC 2.7.7.- (RNA Polymerase II)
[Em] Mês de entrada:1307
[Cu] Atualização por classe:161019
[Lr] Data última revisão:
161019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120816
[St] Status:MEDLINE
[do] DOI:10.1007/s10577-012-9306-z


  3 / 16 MEDLINE  
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[PMID]:19383214
[Au] Autor:Koeller CA
[Ad] Endereço:Department of Clinical Investigation, Madigan Army Medical Center, Tacoma, Washington, USA. craig.koeller@us.army.mil
[Ti] Título:Comparison of buprenorphine and butorphanol analgesia in the eastern red-spotted newt (Notophthalmus viridescens).
[So] Source:J Am Assoc Lab Anim Sci;48(2):171-5, 2009 Mar.
[Is] ISSN:1559-6109
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The experimental use of amphibian models in biomedical research increases yearly, but there is a paucity of reports concerning analgesic use in many of these species. In this study, buprenorphine given by intracoelomic injection and butorphanol added to the tank water were compared for analgesic effect in the eastern red-spotted newt after bilateral forelimb amputations. Newts undergoing anesthesia but not surgery and newts having surgery but not given analgesia postoperatively were used as control groups. Animals were tested for food consumption, spontaneous movement, response to tapping on the tank, response to being touched, and body posture. Both buprenorphine by intracoelomic injection and butorphanol in tank water significantly promoted resumption of normal behavior after bilateral surgical amputation of the forelimbs. The difference between analgesic treatment and no analgesic treatment was maintained until 72 h after surgery.
[Mh] Termos MeSH primário: Analgesia/veterinária
Analgésicos Opioides/farmacologia
Buprenorfina/farmacologia
Butorfanol/farmacologia
Ciência dos Animais de Laboratório/métodos
Notophthalmus
[Mh] Termos MeSH secundário: Analgesia/métodos
Animais
Ingestão de Alimentos/efeitos dos fármacos
Atividade Motora/efeitos dos fármacos
Reflexo de Sobressalto/efeitos dos fármacos
Tato/efeitos dos fármacos
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Analgesics, Opioid); 40D3SCR4GZ (Buprenorphine); QV897JC36D (Butorphanol)
[Em] Mês de entrada:0905
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:090423
[St] Status:MEDLINE


  4 / 16 MEDLINE  
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[PMID]:16716618
[Au] Autor:Berner NJ; Bessay EP
[Ad] Endereço:Department of Biology, University of the South, Sewanee, TN, USA. nberner@sewanee.edu
[Ti] Título:Correlation of seasonal acclimatization in metabolic enzyme activity with preferred body temperature in the Eastern red spotted newt (Notophthalmus viridescens viridescens).
[So] Source:Comp Biochem Physiol A Mol Integr Physiol;144(4):429-36, 2006 Aug.
[Is] ISSN:1095-6433
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Eastern red spotted newts, as aquatic adults, are active year round. They are small and easy to handle, and thus lent themselves to a laboratory study of seasonal changes in preferred body temperature and biochemical acclimatization. We collected newts in summer (n=20), late fall (n=10) and winter (n=5). Ten each of the summer and late fall newts were subjected to an aquatic thermal gradient. Summer newts maintained higher cloacal temperatures than late fall newts (26.8+/-0.5 degrees C and 17.2+/-0.4 degrees C, respectively). In addition, the activity of three muscle metabolic enzymes (cytochrome c oxidase (CCO), citrate synthase (CS) and lactate dehydrogenase (LDH)) was studied in all newts collected. Newts compensated for lower late fall and winter temperatures by increasing the activity of CCO during those seasons over that in summer newts at all assay temperatures (8, 16 and 26 degrees C). The activity of CS was greater in winter over summer newts at 8 and 16 degrees C. No seasonal differences in LDH activity were demonstrated. These data in newts indicate that this amphibian modifies some muscle metabolic enzymes in relation to seasonal changes and can modify its behavioral in a way that correlates with those biochemical changes.
[Mh] Termos MeSH primário: Aclimatação
Temperatura Corporal
Proteínas Musculares/metabolismo
Músculo Esquelético/enzimologia
Notophthalmus/fisiologia
[Mh] Termos MeSH secundário: Animais
Citrato (si)-Sintase/análise
Citrato (si)-Sintase/metabolismo
Complexo IV da Cadeia de Transporte de Elétrons/análise
Complexo IV da Cadeia de Transporte de Elétrons/metabolismo
L-Lactato Desidrogenase/análise
L-Lactato Desidrogenase/metabolismo
Proteínas Musculares/análise
Notophthalmus/metabolismo
Estações do Ano
Regulação para Cima
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Muscle Proteins); EC 1.1.1.27 (L-Lactate Dehydrogenase); EC 1.9.3.1 (Electron Transport Complex IV); EC 2.3.3.1 (Citrate (si)-Synthase)
[Em] Mês de entrada:0609
[Cu] Atualização por classe:061115
[Lr] Data última revisão:
061115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:060524
[St] Status:MEDLINE


  5 / 16 MEDLINE  
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[PMID]:16161011
[Au] Autor:Yang EV; Wang L; Tassava RA
[Ad] Endereço:Department of Molecular Genetics, The Ohio State University, Columbus, Ohio 43210, USA.
[Ti] Título:Effects of exogenous FGF-1 treatment on regeneration of the lens and the neural retina in the newt, Notophthalmus viridescens.
[So] Source:J Exp Zool A Comp Exp Biol;303(10):837-44, 2005 Oct 01.
[Is] ISSN:1548-8969
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Experiments were designed to compare the effects of recombinant newt fibroblast growth factor-1 (rnFGF-1) and recombinant human glial growth factor (rhGGF) on lens and retina regeneration in the eyes of adult newts. Both eyes were retinectomized and lentectomized. Beginning 3 days after the operation, one eye was given either 0.1 microg of rnFGF-1 or 0.1 microg of rhGGF in 1 microl of phosphate-buffered saline (PBS) per injection, three per week. Contralateral operated eyes served as controls and were treated with PBS alone or were not injected. In eyes that were not injected, injected with PBS alone, or with PBS containing rhGGF, regeneration of both the retina and the lens proceeded normally as described in the literature. In these control eyes, the entire retinal pigmented epithelium (RPE) depigmented/dedifferentiated and a retina rudiment formed from which a new retina regenerated by the end of the experiment at day 41 post-operation. Likewise, only a small area of dorsal iris depigmented/dedifferentiated and formed a lens vesicle from which a lens subsequently regenerated. The vitreous remained relatively free of loose cells. In eyes given rnFGF-1, the RPE depigmented/dedifferentiated and formed what appeared to be a retina rudiment but a new retina did not regenerate. Instead, vesicles were seen associated with the retina rudiment. In eyes given rnFGF-1, both the dorsal iris and ventral iris depigmented/dedifferentiated and lens regeneration occurred but the new lenses had abnormal fiber cells and the lens epithelium was very thin or absent. In addition, ectopic lenses usually regenerated in rnFGF-1-treated eyes. An abundance of loose cells were present in the vitreous of rnFGF-1-treated eyes associated largely with the RPE and the dorsal and ventral irises. The results are consistent with the view that the timely expression of FGFs is involved in the depigmentation/dedifferentiation of the RPE and dorsal iris and is necessary for proper regeneration of the lens and neural retina. Continued presence of FGF results in continued and excessive dedifferentiation, resulting in the lack of retina regeneration and abnormal lens regeneration.
[Mh] Termos MeSH primário: Fator 1 de Crescimento de Fibroblastos/farmacologia
Cristalino/efeitos dos fármacos
Notophthalmus/fisiologia
Regeneração/efeitos dos fármacos
Retina/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Seres Humanos
Cristalino/fisiologia
Neuregulina-1/farmacologia
Regeneração/fisiologia
Retina/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Neuregulin-1); 104781-85-3 (Fibroblast Growth Factor 1)
[Em] Mês de entrada:0606
[Cu] Atualização por classe:091103
[Lr] Data última revisão:
091103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:050915
[St] Status:MEDLINE


  6 / 16 MEDLINE  
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[PMID]:11919697
[Au] Autor:Phillips JB; Freake MJ; Fischer JH; Borland C
[Ad] Endereço:Department of Biology, Indiana University, Bloomington, IN 47405, USA. jphillip@vt.edu
[Ti] Título:Behavioral titration of a magnetic map coordinate.
[So] Source:J Comp Physiol A Neuroethol Sens Neural Behav Physiol;188(2):157-60, 2002 Mar.
[Is] ISSN:0340-7594
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Spatial variation in the inclination of the geomagnetic field has been implicated in the map component of homing by eastern red-spotted newts Notophthalmus viridescens. Here we show that when newts are exposed to small changes in magnetic inclination, the most dramatic effects on homing orientation occur at values close to the 'home value', as predicted by the magnetic map hypothesis (Phillips 1996). Newts reverse the direction of homing orientation over a range of inclination of 0.5 degrees spanning the home value, providing further evidence that magnetic inclination or one of its components (i.e., vertical or horizontal intensity) is used to derive map information.
[Mh] Termos MeSH primário: Locomoção
Magnetismo
Notophthalmus/fisiologia
Orientação
[Mh] Termos MeSH secundário: Animais
Masculino
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Em] Mês de entrada:0209
[Cu] Atualização por classe:061115
[Lr] Data última revisão:
061115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:020329
[St] Status:MEDLINE


  7 / 16 MEDLINE  
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[PMID]:11351327
[Au] Autor:Park D; Propper CR
[Ad] Endereço:Department of Biological Sciences, Northern Arizona University, Flagstaff, Arizona 86011, USA. dp27@dana.ucc.nau.edu
[Ti] Título:Repellent function of male pheromones in the red-spotted newt.
[So] Source:J Exp Zool;289(6):404-8, 2001 May 01.
[Is] ISSN:0022-104X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Pheromones act as attractants and sexual stimulants in most vertebrates. For example, in red-spotted newts, Notophthalmus viridescens, female pheromones attract males, and male pheromones increase female receptivity. However, no studies have determined whether male vertebrates produce a pheromone that repels competing males. Through a series of olfactory mate selection tests, we found that sexually motivated male red-spotted newts produce a pheromone that functions to repel other approaching males. Our finding is the first report of a repelling function for pheromones in male vertebrates. The pheromones may act to increase both the sender's and receiver's mating success when the operational sex ratio (OSR) is male biased.
[Mh] Termos MeSH primário: Notophthalmus
Atrativos Sexuais
Comportamento Sexual Animal
[Mh] Termos MeSH secundário: Animais
Masculino
Movimento
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Sex Attractants)
[Em] Mês de entrada:0106
[Cu] Atualização por classe:061115
[Lr] Data última revisão:
061115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:010515
[St] Status:MEDLINE


  8 / 16 MEDLINE  
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[PMID]:9862713
[Au] Autor:Del Rio-Tsonis K; Tsonis PA; Zarkadis IK; Tsagas AG; Lambris JD
[Ad] Endereço:Department of Biology, University of Dayton, OH 45469, USA.
[Ti] Título:Expression of the third component of complement, C3, in regenerating limb blastema cells of urodeles.
[So] Source:J Immunol;161(12):6819-24, 1998 Dec 15.
[Is] ISSN:0022-1767
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In this study we have shown that complement component C3 is expressed in the regenerating tissue during urodele limb regeneration. C3 was expressed in the dedifferentiated regeneration blastema and in the redifferentiated limb tissues in the axolotl, Amblystoma mexicanum, and in Notophthalmus viridescens. This expression was verified by immunofluorescent staining using an Ab against axolotl C3 and by in situ hybridization with an axolotl C3 cDNA probe. In the early stages of regeneration C3 appeared to be equally present in all mesenchymal cells and in the wound epithelium, whereas in the later stages it was mainly expressed in the differentiating muscle cells. Since no expression was seen in the developing limb, it appears that the C3 expression was specific to the regeneration process. We then demonstrated by hybridization experiments that a blastema cell line of myogenic origin expresses C3. All these findings implicate C3 in the dedifferentiation process and may indicate a new role for this molecule in muscle differentiation.
[Mh] Termos MeSH primário: Ambystoma/fisiologia
Complemento C3/biossíntese
Extremidades/fisiologia
Regulação da Expressão Gênica no Desenvolvimento
Notophthalmus/fisiologia
Regeneração/genética
[Mh] Termos MeSH secundário: Ambystoma/genética
Sequência de Aminoácidos
Animais
Sequência de Bases
Diferenciação Celular
Células Cultivadas
Complemento C3/química
Complemento C3/genética
Extremidades/embriologia
Hibridização In Situ
Dados de Sequência Molecular
Proteínas Musculares/biossíntese
Proteínas Musculares/genética
Músculo Esquelético/metabolismo
Músculo Esquelético/fisiologia
Notophthalmus/genética
RNA Mensageiro/biossíntese
RNA Mensageiro/genética
Alinhamento de Sequência
Homologia de Sequência do Ácido Nucleico
Especificidade da Espécie
Xenopus laevis/genética
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, P.H.S.
[Nm] Nome de substância:
0 (Complement C3); 0 (Muscle Proteins); 0 (RNA, Messenger)
[Em] Mês de entrada:9901
[Cu] Atualização por classe:071114
[Lr] Data última revisão:
071114
[Sb] Subgrupo de revista:AIM; IM; S
[Da] Data de entrada para processamento:981223
[St] Status:MEDLINE


  9 / 16 MEDLINE  
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[PMID]:9695841
[Au] Autor:Gall J; Murphy C
[Ad] Endereço:Dept of Embryology, Carnegie Institute of Washington, Baltimore, MD 21210-3399, USA. gall@mail1.ciwemb.edu
[Ti] Título:Pictures in cell biology. Lampbrush chromosomes from sperm chromatin.
[So] Source:Trends Cell Biol;8(5):207, 1998 May.
[Is] ISSN:0962-8924
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Cromatina/genética
Cromossomos/genética
Espermatozoides/citologia
[Mh] Termos MeSH secundário: Animais
Feminino
Masculino
Notophthalmus/genética
Xenopus/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chromatin)
[Em] Mês de entrada:9808
[Cu] Atualização por classe:031114
[Lr] Data última revisão:
031114
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:980808
[St] Status:MEDLINE


  10 / 16 MEDLINE  
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[PMID]:9529374
[Au] Autor:Gall JG; Murphy C
[Ad] Endereço:Department of Embryology, Carnegie Institution, Baltimore, Maryland 21210, USA. gall@mail1.ciwemb.edu
[Ti] Título:Assembly of lampbrush chromosomes from sperm chromatin.
[So] Source:Mol Biol Cell;9(4):733-47, 1998 Apr.
[Is] ISSN:1059-1524
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We have examined the behavior of demembranated sperm heads when injected into the germinal vesicle (GV) of amphibian oocytes. Xenopus sperm heads injected into Xenopus GVs swelled immediately and within hours began to stain with an antibody against RNA polymerase II (Pol II). Over time each sperm head became a loose mass of chromosome-like threads, which by 24-48 h resolved into individually recognizable lampbrush chromosomes (LBCs). Although LBCs derived from sperm are unreplicated single chromatids, their morphology and immunofluorescent staining properties were strikingly similar to those of the endogenous lampbrush bivalents. They displayed typical transcriptionally active loops extending from an axis of condensed chromomeres, as well as locus-specific "landmarks. " Experiments with [3H]GTP and actinomycin D demonstrated that transcription was not necessary for the initial swelling of the sperm heads and acquisition of Pol II but was required for maintenance of the lampbrush loops. Splicing was not required at any stage during formation of sperm LBCs. When Xenopus sperm heads were injected into GVs of the newt Notophthalmus, the resulting sperm LBCs displayed very long loops with pronounced Pol II axes, like those of the endogenous newt LBCs; as expected, they stained with antibodies against newt-specific proteins. Other heterologous injections, including sperm heads of the frog Rana pipiens and the zebrafish Danio rerio in Xenopus GVs, confirm that LBCs can be derived from taxonomically distant organisms. The GV system should help identify both cis- and trans-acting factors needed to convert condensed chromatin into transcriptionally active LBCs. It may also be useful in producing cytologically analyzable chromosomes from organisms whose oocytes do not go through a typical lampbrush phase or cannot be manipulated by current techniques.
[Mh] Termos MeSH primário: Cromatina/genética
Cromossomos
Cabeça do Espermatozoide/fisiologia
[Mh] Termos MeSH secundário: Animais
Cromátides/genética
Cromatina/ultraestrutura
Dactinomicina/farmacologia
Feminino
Guanosina Trifosfato/metabolismo
Masculino
Camundongos
Notophthalmus
Oócitos/fisiologia
RNA Polimerase II/genética
RNA Polimerase II/metabolismo
Processamento de RNA
Cabeça do Espermatozoide/efeitos dos fármacos
Cabeça do Espermatozoide/ultraestrutura
Transcrição Genética/efeitos dos fármacos
Xenopus laevis
Peixe-Zebra
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, P.H.S.
[Nm] Nome de substância:
0 (Chromatin); 1CC1JFE158 (Dactinomycin); 86-01-1 (Guanosine Triphosphate); EC 2.7.7.- (RNA Polymerase II)
[Em] Mês de entrada:9805
[Cu] Atualização por classe:161019
[Lr] Data última revisão:
161019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:980516
[St] Status:MEDLINE



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