Base de dados : MEDLINE
Pesquisa : B01.050.150.900.248.350.650 [Categoria DeCS]
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[PMID]:29197784
[Au] Autor:Banerjee S; Chaturvedi CM
[Ad] Endereço:Department of Zoology, Banaras Hindu University, Varanasi 221005, India.
[Ti] Título:Simulated photoperiod influences testicular activity in quail via modulating local GnRHR-GnIHR, GH-R, Cnx-43 and 14-3-3.
[So] Source:J Photochem Photobiol B;178:412-423, 2018 Jan.
[Is] ISSN:1873-2682
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The hypothalamo-hypophyseal-gonadal axis mediated differential photosexual responses in quail kept under different simulated photoperiodic conditions have been studied in details. Local testicular GnRH-GnIH and their receptor system has been hypothesized to be modulated in quail showing different photo-sexual responses and thus influence the testicular activity and steroidogenesis through local (paracrine and autocrine) action. To validate this hypothesis, we studied the expression of gonadotropin releasing hormone receptor (GnRH-R), gonadotropin inhibiting hormone receptor (GnIH-R) mRNA, growth hormone receptor (GH-R), proliferating cell nuclear antigen (PCNA), 14-3-3, Connexin-43 (Cnx-43), steroidogenic factor-1 (SF-1), Steroidogenic Acute Regulatory protein (StAR), steroidogenic enzyme (3ß HSD) in testis as well as androgen receptor (AR) in testis and epididymis of photosensitive (PS), scotorefractory (SR), photorefractory (PR) and scotosensitive (SS) quail. Experimental findings clearly indicate the increased expression of GnIH-R mRNA and suppression of GnRH-R, GH-R, PCNA, 14-3-3, Connexin-43, SF-1, StAR, 3ß HSD in testis as well as AR in testis and epididymis of PR and SS quail, while PS and SR quail exhibited the opposite results i.e., significantly decreased expression of GnIH-R mRNA and increased expression of GnRH-R, GH-R, PCNA, 14-3-3, Cnx-43, SF-1, StAR, 3ß HSD in testis as well as AR in testis and epididymis. The significantly increased intra-testicular testosterone has been observed in the PS and SR quail while, PR and SS quail showed opposite results. Hence, we conclude that PS and SR quail showed significantly increased testicular activity and steroidogenesis while opposite pattern was observed in PR and SS quail.
[Mh] Termos MeSH primário: Proteínas 14-3-3/metabolismo
Conexina 43/metabolismo
Hormônio Liberador de Gonadotropina/metabolismo
Receptores LHRH/metabolismo
Testículo/metabolismo
[Mh] Termos MeSH secundário: Proteínas 14-3-3/genética
Animais
Conexina 43/genética
Epididimo/metabolismo
Epididimo/patologia
Hormônio Liberador de Gonadotropina/genética
Masculino
Microscopia Confocal
Fotoperíodo
Antígeno Nuclear de Célula em Proliferação/genética
Antígeno Nuclear de Célula em Proliferação/metabolismo
Codorniz
Receptores Androgênicos/genética
Receptores Androgênicos/metabolismo
Receptores LHRH/genética
Fator Esteroidogênico 1/genética
Fator Esteroidogênico 1/metabolismo
Testículo/patologia
Testosterona/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (14-3-3 Proteins); 0 (Connexin 43); 0 (Proliferating Cell Nuclear Antigen); 0 (Receptors, Androgen); 0 (Receptors, LHRH); 0 (Steroidogenic Factor 1); 33515-09-2 (Gonadotropin-Releasing Hormone); 3XMK78S47O (Testosterone)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171204
[St] Status:MEDLINE


  2 / 4211 MEDLINE  
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[PMID]:28465146
[Au] Autor:Tokunaga T; Yamamoto Y; Sakai M; Tomonaga K; Honda T
[Ad] Endereço:Laboratory of RNA Viruses, Department of Virus Research, Institute for Frontier Life and Medical Sciences (InFRONT), Kyoto University, Kyoto, Japan; Department of Mammalian Regulatory Network, Graduate School of Biostudies, Kyoto University, Kyoto, Japan.
[Ti] Título:Antiviral activity of favipiravir (T-705) against mammalian and avian bornaviruses.
[So] Source:Antiviral Res;143:237-245, 2017 07.
[Is] ISSN:1872-9096
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Bornaviruses, non-segmented, negative-strand RNA viruses, are emerging agents with the potential for causing various types of neurological symptoms. Previous studies have shown that ribavirin, a nucleic acid analog with broad-spectrum antiviral activity, has a potent antiviral effect on infections with a mammalian bornavirus, Borna disease virus (BoDV-1), as well as avian bornaviruses. However, ribavirin-based treatment does not eliminate bornaviruses from persistently infected cells and viral replication resumes after treatment cessation. Therefore, the development of a novel effective anti-bornavirus treatment is needed. To identify such agents, we screened nucleoside/nucleotide mimetics for agents with anti-bornavirus activity. We used Vero cells infected with recombinant BoDV-1 carrying Gaussia luciferase to monitor BoDV-1 replication and found that favipiravir (T-705) is a potent inhibitor of BoDV-1 replication. T-705 suppressed BoDV-1 replication in a dose- and time-dependent manner during the observation period of 4 weeks. Notably, no increase in luciferase activity or in the number of BoDV-1-positive cells was detected in the at least 4 weeks following T-705 removal. Finally, we demonstrated that T-705 effectively suppressed viral replication of both BoDV-1 and an avian bornavirus, suggesting that T-705 may have a strong antiviral activity against a broad range of bornaviruses. Our findings provide a novel and effective option for treating persistent bornavirus infection.
[Mh] Termos MeSH primário: Amidas/farmacologia
Antivirais/farmacologia
Bornaviridae/efeitos dos fármacos
Pirazinas/farmacologia
[Mh] Termos MeSH secundário: Amidas/administração & dosagem
Animais
Antivirais/administração & dosagem
Doenças das Aves/virologia
Doença de Borna/tratamento farmacológico
Doença de Borna/virologia
Vírus da Doença de Borna/genética
Bornaviridae/genética
Linhagem Celular
Cercopithecus aethiops
Testes de Sensibilidade Microbiana
Pirazinas/administração & dosagem
Codorniz
RNA Viral/análise
Fatores de Tempo
Células Vero
Carga Viral/efeitos dos fármacos
Replicação Viral/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Amides); 0 (Antiviral Agents); 0 (Pyrazines); 0 (RNA, Viral); EW5GL2X7E0 (favipiravir)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180213
[Lr] Data última revisão:
180213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE


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[PMID]:28926597
[Au] Autor:Reyna KS; Burggren WW
[Ad] Endereço:Developmental Integrative Biology Research Group, Department of Biological Sciences, University of North Texas, Denton, Texas, United States of America.
[Ti] Título:Altered embryonic development in northern bobwhite quail (Colinus virginianus) induced by pre-incubation oscillatory thermal stresses mimicking global warming predictions.
[So] Source:PLoS One;12(9):e0184670, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Global warming is likely to alter reproductive success of ground-nesting birds that lay eggs normally left unattended for days or even weeks before actual parental incubation, especially in already warm climates. The native North American bobwhite quail (Colinus virginianus) is such a species, and pre-incubation quail eggs may experience temperatures ≥45°C. Yet, almost nothing is known about embryonic survival after such high pre-incubation temperatures. Freshly laid bobwhite quail eggs were exposed during a 12 day pre-incubation period to one of five thermal regimes: low oscillating temperatures (25-40°C, mean = 28.9°C), high oscillating temperatures (30-45°C, mean = 33.9°C), low constant temperatures (28.85°C), high constant temperatures (mean = 33.9°C), or commercially employed pre-incubation temperatures (20°C). After treatment, eggs were then incubated at a standard 37.5°C to determine subsequent effects on embryonic development rate, survival, water loss, hatching, and embryonic oxygen consumption. Both quantity of heating degree hours during pre-incubation and specific thermal regime (oscillating vs. non-oscillating) profoundly affected important aspects of embryo survival and indices of development and growth Pre-incubation quail eggs showed a remarkable tolerance to constant high temperatures (up to 45°C), surviving for 4.5±0.3 days of subsequent incubation, but high oscillating pre-incubation temperature increased embryo survival (mean survival 12.2±1.8 days) and led to more rapid development than high constant temperature (maximum 38.5°C), even though both groups experienced the same total heating degree-hours. Oxygen consumption was ~200-300 µl O2.egg.min-1 at hatching in all groups, and was not affected by pre-incubation conditions. Oscillating temperatures, which are the norm for pre-incubation quail eggs in their natural habitat, thus enhanced survival at higher temperatures. However, a 5°C increase in pre-incubation temperature, which equates to the predicted long-term increases of 5°C or more, nonetheless reduced hatching rate by approximately 50%. Thus, while pre-incubation bobwhite eggs may be resiliant to moderate oscillating temperature increases, global warming will likely severely impact wild bobwhite quail populations, especially in their strongholds in southern latitudes.
[Mh] Termos MeSH primário: Codorniz/crescimento & desenvolvimento
Estresse Fisiológico
[Mh] Termos MeSH secundário: Animais
Embrião não Mamífero/metabolismo
Desenvolvimento Embrionário
Aquecimento Global
Estimativa de Kaplan-Meier
Consumo de Oxigênio
Codorniz/fisiologia
Temperatura Ambiente
Água/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
059QF0KO0R (Water)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171020
[Lr] Data última revisão:
171020
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170920
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184670


  4 / 4211 MEDLINE  
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[PMID]:28807901
[Au] Autor:Furutera T; Takechi M; Kitazawa T; Takei J; Yamada T; Vu Hoang T; Rijli FM; Kurihara H; Kuratani S; Iseki S
[Ad] Endereço:Section of Molecular Craniofacial Embryology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan.
[Ti] Título:Differing contributions of the first and second pharyngeal arches to tympanic membrane formation in the mouse and chick.
[So] Source:Development;144(18):3315-3324, 2017 09 15.
[Is] ISSN:1477-9129
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:We have proposed that independent origins of the tympanic membrane (TM), consisting of the external auditory meatus (EAM) and first pharyngeal pouch, are linked with distinctive middle ear structures in terms of dorsal-ventral patterning of the pharyngeal arches during amniote evolution. However, previous studies have suggested that the first pharyngeal arch (PA1) is crucial for TM formation in both mouse and chick. In this study, we compare TM formation along the anterior-posterior axis in these animals using expression as a marker of the second pharyngeal arch (PA2). In chick, the EAM begins to invaginate at the surface ectoderm of PA2, not at the first pharyngeal cleft, and the entire TM forms in PA2. Chick-quail chimera that have lost PA2 and duplicated PA1 suggest that TM formation is achieved by developmental interaction between a portion of the EAM and the columella auris in PA2, and that PA1 also contributes to formation of the remaining part of the EAM. By contrast, in mouse, TM formation is highly associated with an interdependent relationship between the EAM and tympanic ring in PA1.
[Mh] Termos MeSH primário: Região Branquial/embriologia
Membrana Timpânica/embriologia
[Mh] Termos MeSH secundário: Animais
Região Branquial/metabolismo
Embrião de Galinha
Galinhas
Meato Acústico Externo/embriologia
Orelha Média/embriologia
Embrião de Mamíferos/metabolismo
Proteínas de Fluorescência Verde/metabolismo
Proteínas de Homeodomínio/metabolismo
Camundongos
Camundongos Knockout
Modelos Biológicos
Fenótipo
Codorniz/embriologia
Membrana Timpânica/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Homeodomain Proteins); 0 (Hoxa2 protein, mouse); 0 (enhanced green fluorescent protein); 147336-22-9 (Green Fluorescent Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171126
[Lr] Data última revisão:
171126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170816
[St] Status:MEDLINE
[do] DOI:10.1242/dev.149765


  5 / 4211 MEDLINE  
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[PMID]:28795263
[Au] Autor:Fan L; Li Z; Huang J; Yang Z; Xiao S; Wang X; Dang R; Zhang S
[Ad] Endereço:College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, Shaanxi, China.
[Ti] Título:Dynamic distribution and tissue tropism of avian encephalomyelitis virus isolate XY/Q-1410 in experimentally infected Korean quail.
[So] Source:Arch Virol;162(11):3447-3458, 2017 Nov.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Avian encephalomyelitis (AE) is an important infectious poultry disease worldwide that is caused by avian encephalomyelitis virus (AEV). However, to date, the dynamic distribution of AEV in quails has not been well described. Quantitative real-time polymerase chain reaction (qPCR) and immunohistochemistry (IHC) assays were used to investigate the dynamic distribution and tissue tropism of AEV in experimentally infected Korean quail. AEV was detected in the cerebrum, cerebellum, proventriculus, intestine, liver, pancreas, spleen, bursa, lung and kidney as early as 3 days post-infection (dpi). The viral loads in the proventriculus, intestine, spleen and bursa were relatively higher than in other tissues. According to the qPCR results, AEV XY/Q-1410 infection lasted for at least 60 days in infected Korean quail. Immunohistochemistry-positive staining signals of AEV antigen were analysed by Image-Pro Plus software. A positive correlation between qPCR and IHC results was identified in most tissues. Our results provide an insight into the dynamic distribution of AEV in various tissues after infection. The distinct dynamic distribution of the viral genome in Korean quail in the early and late stages of infection suggests that AEV replication is affected by antibody levels and the maturity of the immune system of the host.
[Mh] Termos MeSH primário: Vírus da Encefalomielite Aviária/fisiologia
Infecções por Picornaviridae/veterinária
Doenças das Aves Domésticas/virologia
Codorniz
Tropismo Viral/fisiologia
[Mh] Termos MeSH secundário: Animais
Encéfalo/virologia
Bolsa de Fabricius/virologia
Intestinos/virologia
Fígado/virologia
Pâncreas/virologia
Infecções por Picornaviridae/virologia
Proventrículo/virologia
Baço/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171025
[Lr] Data última revisão:
171025
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170811
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3504-0


  6 / 4211 MEDLINE  
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[PMID]:28763502
[Au] Autor:Martín-Estebané M; Navascués J; Sierra-Martín A; Martín-Guerrero SM; Cuadros MA; Carrasco MC; Marín-Teva JL
[Ad] Endereço:Departamento de Biología Celular, Facultad de Ciencias, Universidad de Granada, Granada, Spain.
[Ti] Título:Onset of microglial entry into developing quail retina coincides with increased expression of active caspase-3 and is mediated by extracellular ATP and UDP.
[So] Source:PLoS One;12(8):e0182450, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Microglial cell precursors located in the area of the base of the pecten and the optic nerve head (BP/ONH) start to enter the retina of quail embryos at the 7th day of incubation (E7), subsequently colonizing the entire retina by central-to-peripheral tangential migration, as previously shown by our group. The present study demonstrates a precise chronological coincidence of the onset of microglial cell entry into the retina with a striking increase in death of retinal cells, as revealed by their active caspase-3 expression and TUNEL staining, in regions dorsal to the BP/ONH area, suggesting that dying retinal cells would contribute to the microglial cell inflow into the retina. However, the molecular mechanisms involved in this inflow are currently unclear. Extracellular nucleotides, such as ATP and UDP, have previously been shown to favor migration of microglia towards brain injuries because they are released by apoptotic cells and stimulate both chemotaxis and chemokinesis in microglial cells via signaling through purinergic receptors. Hence, we tested here the hypothesis that ATP and UDP play a role in the entry and migration of microglial precursors into the developing retina. For this purpose, we used an experimental model system based on organotypic cultures of E6.5 quail embryo retina explants, which mimics the entry and migration of microglial precursors in the in situ developing retina. Inhibition of purinergic signaling by treating retina explants with either apyrase, a nucleotide-hydrolyzing enzyme, or suramin, a broad spectrum antagonist of purinergic receptors, significantly prevents the entry of microglial cells into the retina. In addition, treatment of retina explants with either exogenous ATP or UDP results in significantly increased numbers of microglial cells entering the retina. In light of these findings, we conclude that purinergic signaling by extracellular ATP and UDP is necessary for the entry and migration of microglial cells into the embryonic retina by inducing chemokinesis in these cells.
[Mh] Termos MeSH primário: Trifosfato de Adenosina/metabolismo
Caspase 3/metabolismo
Regulação da Expressão Gênica no Desenvolvimento
Microglia/citologia
Retina/embriologia
Difosfato de Uridina/metabolismo
[Mh] Termos MeSH secundário: Animais
Sobrevivência Celular
Quimiotaxia
Ativação Enzimática
Microscopia Confocal
Nervo Óptico/patologia
Codorniz
Receptores Purinérgicos/metabolismo
Retina/fisiologia
Transdução de Sinais
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Receptors, Purinergic); 58-98-0 (Uridine Diphosphate); 8L70Q75FXE (Adenosine Triphosphate); EC 3.4.22.- (Caspase 3)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170802
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182450


  7 / 4211 MEDLINE  
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[PMID]:28684624
[Au] Autor:Hintze M; Prajapati RS; Tambalo M; Christophorou NAD; Anwar M; Grocott T; Streit A
[Ad] Endereço:Department of Craniofacial Development & Stem Cell Biology, King's College London, Dental Institute, London SE1 9RT, UK.
[Ti] Título:Cell interactions, signals and transcriptional hierarchy governing placode progenitor induction.
[So] Source:Development;144(15):2810-2823, 2017 08 01.
[Is] ISSN:1477-9129
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In vertebrates, cranial placodes contribute to all sense organs and sensory ganglia and arise from a common pool of Six1/Eya2+ progenitors. Here we dissect the events that specify ectodermal cells as placode progenitors using newly identified genes upstream of the Six/Eya complex. We show in chick that two different tissues, namely the lateral head mesoderm and the prechordal mesendoderm, gradually induce placode progenitors: cells pass through successive transcriptional states, each identified by distinct factors and controlled by different signals. Both tissues initiate a common transcriptional state but over time impart regional character, with the acquisition of anterior identity dependent on Shh signalling. Using a network inference approach we predict the regulatory relationships among newly identified transcription factors and verify predicted links in knockdown experiments. Based on this analysis we propose a new model for placode progenitor induction, in which the initial induction of a generic transcriptional state precedes regional divergence.
[Mh] Termos MeSH primário: Transdução de Sinais/fisiologia
Vertebrados/embriologia
[Mh] Termos MeSH secundário: Animais
Comunicação Celular/genética
Comunicação Celular/fisiologia
Embrião de Galinha
Galinhas
Ectoderma/citologia
Ectoderma/embriologia
Ectoderma/metabolismo
Eletroporação
Gânglios Sensitivos/citologia
Gânglios Sensitivos/embriologia
Gânglios Sensitivos/metabolismo
Regulação da Expressão Gênica no Desenvolvimento/genética
Regulação da Expressão Gênica no Desenvolvimento/fisiologia
Hibridização In Situ
Análise de Sequência com Séries de Oligonucleotídeos
Codorniz
Órgãos dos Sentidos/citologia
Órgãos dos Sentidos/embriologia
Órgãos dos Sentidos/metabolismo
Transdução de Sinais/genética
Fatores de Transcrição/genética
Fatores de Transcrição/metabolismo
Vertebrados/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Transcription Factors)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171126
[Lr] Data última revisão:
171126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170708
[St] Status:MEDLINE
[do] DOI:10.1242/dev.147942


  8 / 4211 MEDLINE  
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[PMID]:28418272
[Au] Autor:Marks C; Nickles NE; Wise T; Mavroidis S
[Ad] Endereço:Department of Biology, University of Mount Union, Alliance, Ohio.
[Ti] Título:Incubation Temperature Alters Temperature-Dependent Oxygen Consumption in Northern Bobwhite Quail Hatchlings (Colinus virginianus).
[So] Source:Physiol Biochem Zool;90(4):478-483, 2017 Jul/Aug.
[Is] ISSN:1537-5293
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This study investigated the effect of mismatching incubation and posthatch temperatures in northern bobwhite quail hatchlings. Quail embryos were incubated at 35.5° or 37.5°C. Metabolic rates were then measured in hatchlings acclimated to either the same or the opposite temperature treatment. While hatchlings expressed higher oxygen consumption when posthatch temperature did not match incubation temperature, the effect of mismatching temperatures was significant only when posthatch temperature was higher than incubation temperature. Our data suggest that bobwhite quail hatchlings may express increased metabolism due to mismatches between incubation and posthatch temperatures. More specifically, the nature or direction of the mismatch can determine the magnitude of the metabolic effect. These findings highlight the importance of considering the context of specific conditions experienced throughout ontogeny when observing phenotypic outcomes.
[Mh] Termos MeSH primário: Animais Recém-Nascidos/fisiologia
Regulação da Temperatura Corporal/fisiologia
Consumo de Oxigênio/fisiologia
Codorniz/fisiologia
[Mh] Termos MeSH secundário: Aclimatação
Animais
Óvulo/fisiologia
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170921
[Lr] Data última revisão:
170921
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170419
[St] Status:MEDLINE
[do] DOI:10.1086/692127


  9 / 4211 MEDLINE  
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[PMID]:28414820
[Au] Autor:Wang P; Qiu Z; Xia D; Tang S; Shen X; Zhao Q
[Ad] Endereço:School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu, China.
[Ti] Título:Transcriptome analysis of the epidermis of the purple quail-like (q-lp) mutant of silkworm, Bombyx mori.
[So] Source:PLoS One;12(4):e0175994, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A new purple quail-like (q-lp) mutant found from the plain silkworm strain 932VR has pigment dots on the epidermis similar to the pigment mutant quail (q). In addition, q-lp mutant larvae are inactive, consume little and grow slowly, with a high death rate and other developmental abnormalities. Pigmentation of the silkworm epidermis consists of melanin, ommochrome and pteridine. Silkworm development is regulated by ecdysone and juvenile hormone. In this study, we performed RNA-Seq on the epidermis of the q-lp mutant in the 4th instar during molting, with 932VR serving as the control. The results showed 515 differentially expressed genes, of which 234 were upregulated and 281 downregulated in q-lp. BLASTGO analysis indicated that the downregulated genes mainly encode protein-binding proteins, membrane components, oxidation/reduction enzymes, and proteolytic enzymes, whereas the upregulated genes largely encode cuticle structural constituents, membrane components, transport related proteins, and protein-binding proteins. Quantitative reverse transcription PCR was used to verify the accuracy of the RNA-Seq data, focusing on key genes for biosynthesis of the three pigments and chitin as well as genes encoding cuticular proteins and several related nuclear receptors, which are thought to play key roles in the q-lp mutant. We drew three conclusions based on the results: 1) melanin, ommochrome and pteridine pigments are all increased in the q-lp mutant; 2) more cuticle proteins are expressed in q-lp than in 932VR, and the number of upregulated cuticular genes is significantly greater than downregulated genes; 3) the downstream pathway regulated by ecdysone is blocked in the q-lp mutant. Our research findings lay the foundation for further research on the developmental changes responsible for the q-lp mutant.
[Mh] Termos MeSH primário: Bombyx/genética
Epiderme/metabolismo
Codorniz/genética
Transcriptoma/genética
[Mh] Termos MeSH secundário: Animais
Bombyx/metabolismo
Ecdisona/genética
Perfilação da Expressão Gênica/métodos
Genes de Insetos/genética
Proteínas de Insetos/genética
Larva/genética
Melaninas/genética
Mutação/genética
Fenotiazinas/metabolismo
Pigmentação/genética
Pteridinas/metabolismo
RNA/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insect Proteins); 0 (Melanins); 0 (Phenothiazines); 0 (Pteridines); 0 (ommochrome); 3604-87-3 (Ecdysone); 63231-63-0 (RNA)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170505
[Lr] Data última revisão:
170505
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170418
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0175994


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[PMID]:28413083
[Au] Autor:Du ZH; Xia J; Sun XC; Li XN; Zhang C; Zhao HS; Zhu SY; Li JL
[Ad] Endereço:College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China.
[Ti] Título:A novel nuclear xenobiotic receptors (AhR/PXR/CAR)-mediated mechanism of DEHP-induced cerebellar toxicity in quails (Coturnix japonica) via disrupting CYP enzyme system homeostasis.
[So] Source:Environ Pollut;226:435-443, 2017 Jul.
[Is] ISSN:1873-6424
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Di-(2-ethylhexyl)-phthalate (DEHP) is causing serious health hazard in wildlife animal and human through environment and food chain, including the effect of brain development and impacted neurobehavioral outcomes. However, DEHP exposure caused cerebellar toxicity in bird remains unclear. To evaluate DEHP-exerted potential neurotoxicity in cerebellum, male quails were exposed with 0, 250, 500 and 750 mg/kg BW/day DEHP by gavage treatment for 45 days. Neurobehavioral abnormality and cerebellar histopathological alternation were observed in DEHP-induced quails. DEHP exposure increased the contents of total Cytochrome P450s (CYPs) and Cytochrome b5 (Cyt b5) and the activities of NADPH-cytochrome c reductase (NCR) and aniline-4-hydeoxylase (AH) in quail cerebellum. The expression of nuclear xenobiotic receptors (NXRs) and the transcriptions of CYP enzyme isoforms were also influenced in cerebellum by DEHP exposure. These results suggested that DEHP exposure caused the toxic effects of quail cerebellum. DEHP exposure disrupted the cerebellar CYP enzyme system homeostasis via affecting the transcription of CYP enzyme isoforms. The cerebellar P450arom and CYP3A4 might be biomarkers in evaluating the neurotoxicity of DEHP in bird. Finally, this study provided new evidence that DEHP-induced toxic effect of quail cerebellum was associated with activating the NXRs responses and disrupting the CYP enzyme system homeostasis.
[Mh] Termos MeSH primário: Coturnix/fisiologia
Sistema Enzimático do Citocromo P-450/metabolismo
Dietilexilftalato/toxicidade
[Mh] Termos MeSH secundário: Animais
Aromatase/metabolismo
Homeostase/efeitos dos fármacos
Seres Humanos
Masculino
Codorniz/fisiologia
Receptores de Hidrocarboneto Arílico/metabolismo
Receptores Citoplasmáticos e Nucleares/metabolismo
Xenobióticos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Receptors, Aryl Hydrocarbon); 0 (Receptors, Cytoplasmic and Nuclear); 0 (Xenobiotics); 9035-51-2 (Cytochrome P-450 Enzyme System); C42K0PH13C (Diethylhexyl Phthalate); EC 1.14.14.1 (Aromatase)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170706
[Lr] Data última revisão:
170706
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170418
[St] Status:MEDLINE



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