Base de dados : MEDLINE
Pesquisa : B01.050.150.900.493.080.148 [Categoria DeCS]
Referências encontradas : 1268 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 127 ir para página                         

  1 / 1268 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27773705
[Au] Autor:Gao S; Liu S; Yao J; Li N; Yuan Z; Zhou T; Li Q; Liu Z
[Ad] Endereço:The Fish Molecular Genetics and Biotechnology Laboratory, Aquatic Genomics Unit, School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL 36849, USA.
[Ti] Título:Genomic organization and evolution of olfactory receptors and trace amine-associated receptors in channel catfish, Ictalurus punctatus.
[So] Source:Biochim Biophys Acta;1861(3):644-651, 2017 03.
[Is] ISSN:0006-3002
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Channel catfish (Ictalurus punctatus) live in turbid waters with limited visibility to chase prey within a certain distance. This can be compensated through detecting specific water-soluble substances by the olfactory receptors (ORs) and trace amine associated receptors (TAARs) expressed on the olfactory epithelium. METHODS: We identified the OR and TAAR repertoires in channel catfish, and characterized the genomic organizations of these two gene families by data mining available genomic resources. RESULTS: A total of 47 putative OR genes and 36 putative TAAR genes were identified in the channel catfish genome, including 27 functional OR genes and 28 functional TAAR genes. Phylogenetic and orthogroup analyses were conducted to illustrate the evolutionary dynamics of the vertebrate ORs and TAARs. Collinear analysis revealed the presence of two conserved orthologous blocks that contain OR genes between the catfish genome and zebrafish genome. The complete loss of a conserved motif in fish OR family H may contribute to the divergence of family H from other families. The dN/dS analysis indicated that the highest degree of selection pressure was imposed on TAAR subfamily 14 among all fish ORs and TAARs. CONCLUSIONS: The present study provides understanding of the evolutionary dynamics of the two gene families (OR and TAAR) associated with olfaction in channel catfish. GENERAL SIGNIFICANCE: This is the first systematic study of ORs and TAARs in catfish, which could provide valuable genomic resources for further investigation of olfactory mechanisms in teleost fish.
[Mh] Termos MeSH primário: Aminas/metabolismo
Peixes-Gato/genética
Genoma/genética
Ictaluridae/genética
Receptores Acoplados a Proteínas-G/genética
Receptores Odorantes/genética
[Mh] Termos MeSH secundário: Animais
Peixes-Gato/metabolismo
Evolução Molecular
Genômica/métodos
Ictaluridae/metabolismo
Neurônios Receptores Olfatórios/metabolismo
Filogenia
Peixe-Zebra/genética
Peixe-Zebra/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Amines); 0 (Receptors, G-Protein-Coupled); 0 (Receptors, Odorant)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171224
[Lr] Data última revisão:
171224
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


  2 / 1268 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28873591
[Au] Autor:Duan R; Zhang J; Liu L; Cui W; Regenstein JM
[Ad] Endereço:College of Marine Life and Fisheries, Huaihai Institute of Technology, 59 Cangwu Road, Haizhou, 222005, China; Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening, Huaihai Institute of Technology, 59 Cangwu Road, Haizhou, 222005, China. Electronic address: duanruihhit@126.com.
[Ti] Título:The functional properties and application of gelatin derived from the skin of channel catfish (Ictalurus punctatus).
[So] Source:Food Chem;239:464-469, 2018 Jan 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The objective of this work was to extract gelatin from skin of channel catfish (Ictalurus punctatus) and to study its functional properties and application in ice-cream and beer. SDS-PAGE patterns showed that channel catfish gelatin (CCG) had more high molecular weight components (ß and γ chains) than had calf bone gelatin (CBG). The viscosity of CCG was 42mPa.s at 10°C, which was three times more than that of CBG. Compared to CBG, CCG presented higher emulsion capacity and stability, as well as higher foaming stability. CCG conferred the ice-cream samples and beer with better mouth feel and clarification effect, respectively. The results indicated that CCG had great potential to be utilized in the food industry.
[Mh] Termos MeSH primário: Ictaluridae
[Mh] Termos MeSH secundário: Animais
Bovinos
Eletroforese em Gel de Poliacrilamida
Gelatina
Pele
Viscosidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9000-70-8 (Gelatin)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE


  3 / 1268 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28425428
[Au] Autor:Ourth DD; Marecaux E; Raghu D; Peterson BC
[Ad] Endereço:Department of Biological Sciences, The University of Memphis, Memphis, TN 38152, USA.
[Ti] Título:Innate immune response of channel catfish Ictalurus punctatus mannose-binding lectin to channel catfish virus (CCV).
[So] Source:Dis Aquat Organ;124(2):159-163, 2017 04 20.
[Is] ISSN:0177-5103
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The channel catfish virus (CCV) is a pathogenic herpesvirus that infects channel catfish Ictalurus punctatus in pond aquaculture in the southeastern USA. Mannose-binding lectin (MBL), an innate immune protein, could play an important role in the innate response of channel catfish by binding to CCV. Cell cultures of CCV were grown in channel catfish ovary cells (CCOC). A dot-immunoblot enzyme-linked immunosorbent assay was done to determine the binding ability of 5 mo old channel catfish serum MBL (26.2 µg ml-1) to CCOC infected with CCV. Two separate nitrocellulose membrane blotting techniques were done using uninfected and infected CCOC. The uninfected CCOC decreased by 29.3 and 33.4% in their binding of channel catfish MBL when compared with infected CCOC using the 2 membrane procedures. The combined average binding ability of channel catfish MBL towards infected CCOC was therefore 31.4% greater when comparing the infected and uninfected CCOC. Normalization equation values of MBL for the 5 mo old catfish were compared for the 2 membrane binding procedures. The 2 normalization values were very close (142 and 150) in binding ability of MBL to the infected CCOC. The 5 mo catfish serum had twice the concentration of MBL (26.2 µg ml-1) compared to 7 mo catfish serum (13.2 µg ml-1), and the binding percentage of 5 mo serum was 2.4 times greater in infected than in uninfected cells. This demonstrates that the binding of channel catfish serum MBL to CCV is concentration dependent and is related to serum concentrations of MBL.
[Mh] Termos MeSH primário: Infecções por Herpesviridae/veterinária
Ictaluridae/sangue
Ictalurivirus/imunologia
Imunidade Inata/fisiologia
Lectina de Ligação a Manose/fisiologia
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Ensaio de Imunoadsorção Enzimática
Feminino
Infecções por Herpesviridae/virologia
Immunoblotting
Lectina de Ligação a Manose/sangue
Ovário/citologia
Ligação Proteica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Mannose-Binding Lectin)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170629
[Lr] Data última revisão:
170629
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170421
[St] Status:MEDLINE
[do] DOI:10.3354/dao03109


  4 / 1268 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28340427
[Au] Autor:Zhu J; Wang H; Wang J; Wang X; Peng S; Geng Y; Wang K; Ouyang P; Li Z; Huang X; Chen D
[Ad] Endereço:Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, Wenjiang District, Chengdu 611130, China. Electronic address: jieyaozhu@yahoo.com.
[Ti] Título:Identification and characterization of a ß-defensin gene involved in the immune defense response of channel catfish, Ictalurus punctatus.
[So] Source:Mol Immunol;85:256-264, 2017 May.
[Is] ISSN:1872-9142
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Antimicrobial peptides are small peptides that play important roles in a host's innate immune response. As an important antimicrobial peptide, ß-defensin widely distribute in mammals, insects and plants with broad-spectrum antimicrobial activity. In this study, the ß-defensin gene of the channel catfish, Ictalurus punctatus, was cloned, sequenced, and subjected to a bioinformatic analysis. The ß-defensin gene of the channel catfish contains three exons and two introns, and encodes a precursor peptide consisting of two domains: a signal peptide of 24 amino acid residues and a mature peptide of 43 amino acid residues. The mature peptide is estimated to have a molecular mass of 7.1kDa and a theoretical isoelectric point of 8.21. Channel catfish ß-defensin (ccBD) has six conserved cysteine residues, forming three disulfide bridges at C1-C5, C2-C4, and C3-C6, and a ß-sheet in the predicted three-dimensional structure. A phylogenetic analysis suggests that ccBD belongs to the type 1 ß-defensins. Real-time quantitative PCR showed that channel catfish ß-defensin transcripts are constitutively expressed in various tissues in healthy fish, with highest expression in the skin. The expression of ccBD in vivo increased significantly in the head kidney (2.9-fold), gill (2.2-fold), and skin (6.6-fold) at 48h after bacterial (Edwardsiella ictaluri) challenge. In vitro, lipopolysaccharide (LPS), a bacterial mimic, induced significant changes in ccBD expression in leukocytes from the spleen (3.4-fold) and head kidney (3.9-fold) 24h after stimulation. Chemically synthesized ccBD displayed marked inhibitory activity against a broad range of bacteria. These results suggest that ccBD is involved in the innate antibacterial defenses of the channel catfish.
[Mh] Termos MeSH primário: Ictaluridae/genética
Ictaluridae/imunologia
beta-Defensinas/genética
beta-Defensinas/imunologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Sequência de Bases
Clonagem Molecular
Doenças dos Peixes/imunologia
Proteínas de Peixes/genética
Proteínas de Peixes/imunologia
Reação em Cadeia da Polimerase em Tempo Real
Alinhamento de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fish Proteins); 0 (beta-Defensins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170325
[St] Status:MEDLINE


  5 / 1268 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28289846
[Au] Autor:Li Y; Geng X; Bao L; Elaswad A; Huggins KW; Dunham R; Liu Z
[Ad] Endereço:Aquatic Genomics Unit, Fish Molecular Genetics and Biotechnology Laboratory, School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL, 36849, USA.
[Ti] Título:A deletion in the Hermansky-Pudlak syndrome 4 (Hps4) gene appears to be responsible for albinism in channel catfish.
[So] Source:Mol Genet Genomics;292(3):663-670, 2017 Jun.
[Is] ISSN:1617-4623
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Albinism is caused by a series of genetic abnormalities leading to reduction of melanin production. Albinism is quite frequent in catfish, but the causative gene and the molecular basis were unknown. In this study, we conducted a genome-wide association study (GWAS) using the 250 K SNP array. The GWAS analysis allowed mapping of the albino phenotype in the Hermansky-Pudlak syndrome 4 (Hps4) gene, which is known to be involved in melanosome biosynthesis. Sequencing analysis revealed that a 99-bp deletion was present in all analyzed albino catfish at the intron 2 and exon 3 junction. This deletion led to the skipping of the entire exon 3 which was confirmed by RT-PCR. Therefore, Hps4 was determined to be the candidate gene of the catfish albinism.
[Mh] Termos MeSH primário: Proteínas de Peixes/genética
Síndrome de Hermanski-Pudlak/genética
Ictaluridae/genética
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
Estudo de Associação Genômica Ampla
Genótipo
Melaninas/biossíntese
Fenótipo
Polimorfismo de Nucleotídeo Único/genética
Análise de Sequência de DNA
Deleção de Sequência/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fish Proteins); 0 (Melanins)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171007
[Lr] Data última revisão:
171007
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170315
[St] Status:MEDLINE
[do] DOI:10.1007/s00438-017-1302-8


  6 / 1268 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28288365
[Au] Autor:Tian C; Tan S; Bao L; Zeng Q; Liu S; Yang Y; Zhong X; Liu Z
[Ad] Endereço:The Fish Molecular Genetics and Biotechnology Laboratory, Aquatic Genomics Unit, School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL 36849, USA; College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
[Ti] Título:DExD/H-box RNA helicase genes are differentially expressed between males and females during the critical period of male sex differentiation in channel catfish.
[So] Source:Comp Biochem Physiol Part D Genomics Proteomics;22:109-119, 2017 Jun.
[Is] ISSN:1878-0407
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:DExD/H-box RNA helicases are motor proteins participating in nearly all aspects of cellular processes, especially in RNA metabolism. In this study, a total of 54 DExD/H-box RNA helicase genes including 37 DDX (DEAD-box) and 17 DHX (DEAH-box) genes were characterized in channel catfish (Ictalurus punctatus), and annotated through phylogenetic and syntenic analyses. All the catfish RNA helicases contained conserved helicase signature motifs, demonstrating that the RNA helicase gene family was highly conserved. Analysis of the relative rates of synonymous (dS) and nonsynonymous (dN) substitutions revealed that the RNA helicase genes were subjected to strong negative (purifying) selection. Meta-analysis was conducted to determine expression of the RNA helicase genes during the critical period (90-110days post-fertilization, dpf) of male gonad differentiation. At 90dpf, 24 RNA helicase genes were highly differentially expressed in the gonad tissues between the males and females; similarly, 24 and 18 RNA helicase genes were found highly differentially expressed in the gonad tissues between the males and females at 100 and 110dpf, respectively (p<0.01). In general, the vast majority of the RNA helicase genes (31) were expressed at higher levels in females than in males. In the male gonad, a set of 8 RNA helicases were expressed at a significantly higher level at 110dpf than at 90dpf. These findings suggested that RNA helicases may play important roles in sex development and differentiation in teleosts.
[Mh] Termos MeSH primário: RNA Helicases DEAD-box/genética
Ictaluridae/genética
Diferenciação Sexual/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
RNA Helicases DEAD-box/classificação
Feminino
Ictaluridae/crescimento & desenvolvimento
Masculino
Filogenia
Homologia de Sequência de Aminoácidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 3.6.4.13 (DEAD-box RNA Helicases)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170814
[Lr] Data última revisão:
170814
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170314
[St] Status:MEDLINE


  7 / 1268 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27919758
[Au] Autor:Fu Q; Zeng Q; Li Y; Yang Y; Li C; Liu S; Zhou T; Li N; Yao J; Jiang C; Li D; Liu Z
[Ad] Endereço:State Key Laboratory of Estuarine and Coastal Research, East China Normal University, Shanghai 200062, China; The Fish Molecular Genetics and Biotechnology Laboratory, Aquatic Genomics Unit, School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL 36849, USA.
[Ti] Título:The chemokinome superfamily in channel catfish: I. CXC subfamily and their involvement in disease defense and hypoxia responses.
[So] Source:Fish Shellfish Immunol;60:380-390, 2017 Jan.
[Is] ISSN:1095-9947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Chemokines are a superfamily of structurally related chemotactic cytokines exerting significant roles in regulating cell migration and activation. They are defined by the presence of four conserved cysteine residues and are divided into four subfamilies depending on the arrangement of the first two conserved cysteines residues: CXC, CC, C and CX3C. In this study, a complete set of 17 CXC chemokine ligand (CXCL) genes was systematically identified and characterized from channel catfish genome through data mining of existing genomic resources. Phylogenetic analysis allowed annotation of the 17 CXC chemokines. Extensive comparative genomic analyses supported their annotations and orthologies, revealing the existence of fish-specific CXC chemokines and the expansion of CXC chemokines in the teleost genomes. The analysis of gene expression after bacterial infection indicated the CXC chemokines were expressed in a gene-specific manner. CXCL11.3 and CXCL20.3 were expressed significantly higher in resistant fish than in susceptible fish after ESC infection, while CXCL20.2 were expressed significantly higher in resistant fish than in susceptible fish after columnaris infection. The expression of those CXC chemokines, therefore can be a useful indicator of disease resistance. A similar pattern of expression was observed between resistant and susceptible fish with biotic and abiotic stresses, ESC, columnaris and hypoxia, suggesting that high levels of expression of the majority of CXC chemokines, with exception of CXC11 and CXC20, are detrimental to the host.
[Mh] Termos MeSH primário: Quimiocinas CXC/genética
Infecções por Enterobacteriaceae/veterinária
Doenças dos Peixes/genética
Proteínas de Peixes/genética
Infecções por Flavobacteriaceae/veterinária
Expressão Gênica
Ictaluridae
[Mh] Termos MeSH secundário: Anaerobiose/genética
Animais
Quimiocinas CXC/química
Quimiocinas CXC/metabolismo
Resistência à Doença/genética
Edwardsiella ictaluri/fisiologia
Infecções por Enterobacteriaceae/genética
Infecções por Enterobacteriaceae/imunologia
Infecções por Enterobacteriaceae/microbiologia
Doenças dos Peixes/imunologia
Doenças dos Peixes/microbiologia
Proteínas de Peixes/química
Proteínas de Peixes/metabolismo
Infecções por Flavobacteriaceae/genética
Infecções por Flavobacteriaceae/imunologia
Infecções por Flavobacteriaceae/microbiologia
Flavobacterium/fisiologia
Filogenia
Análise de Sequência de DNA/veterinária
Sintenia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chemokines, CXC); 0 (Fish Proteins)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170327
[Lr] Data última revisão:
170327
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161207
[St] Status:MEDLINE


  8 / 1268 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27908977
[Au] Autor:Yoshida MA; Yamamoto D; Sato K
[Ad] Endereço:Atmosphere and Ocean Research Institute, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8564, Japan makoto.a.yoshida@gmail.com.
[Ti] Título:Physostomous channel catfish, , modify swimming mode and buoyancy based on flow conditions.
[So] Source:J Exp Biol;220(Pt 4):597-606, 2017 Feb 15.
[Is] ISSN:1477-9145
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The employment of gliding in aquatic animals as a means of conserving energy has been theoretically predicted and discussed for decades. Several studies have shown that some species glide, whereas others do not. Freshwater fish species that widely inhabit both lentic and lotic environments are thought to be able to adapt to fluctuating flow conditions in terms of locomotion. In adapting to the different functional demands of lentic and lotic environments on fish energetics, physostomous (open swim bladder) fish may optimise their locomotion and activity by controlling their net buoyancy; however, few buoyancy studies have been conducted on physostomous fish in the wild. We deployed accelerometers on free-ranging channel catfish, , in both lentic and lotic environments to quantify their swimming activity, and to determine their buoyancy condition preferences and whether gliding conserves energy. Individual comparisons of swimming efforts between ascent and descent phases revealed that all fish in the lentic environment had negative buoyancy. However, all individuals showed many descents without gliding phases, which was contrary to the behaviour predicted to minimise the cost of transport. The fact that significantly fewer gliding phases were observed in the lotic environment, together with the existence of neutrally buoyant fish, indicated that channel catfish seem to optimise their locomotion through buoyancy control based on flow conditions. The buoyancy optimisation of channel catfish relative to the flow conditions that they inhabit not only reflects differences in swimming behaviour but also provides new insights into the adaptation of physostome fish species to various freshwater environments.
[Mh] Termos MeSH primário: Ictaluridae/fisiologia
Natação
[Mh] Termos MeSH secundário: Animais
Fenômenos Biomecânicos
Feminino
Masculino
Modelos Biológicos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170822
[Lr] Data última revisão:
170822
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161203
[St] Status:MEDLINE
[do] DOI:10.1242/jeb.140202


  9 / 1268 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27818340
[Au] Autor:Zhang D; Beck BH; Lange M; Zhao H; Thongda W; Ye Z; Li C; Peatman E
[Ad] Endereço:School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL 36849, USA.
[Ti] Título:Impact of oral and waterborne administration of rhamnolipids on the susceptibility of channel catfish (Ictalurus punctatus) to Flavobacterium columnare infection.
[So] Source:Fish Shellfish Immunol;60:44-49, 2017 Jan.
[Is] ISSN:1095-9947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Flavobacterium columnare is the causative agent of columnaris disease and causes tremendous morbidity and mortality of farmed fish globally. Previously, we identified a potential lectin-mediator (a rhamnose-binding lectin; RBL1a) of F. columnare adhesion and showed higher RBL1a expression in susceptible channel catfish under basal conditions and following infection. Exposure of challenged fish to the carbohydrate ligand l-rhamnose just prior to a challenge substantially decreased columnaris mortality and pathogen adherence via the down-regulation of RBL1a. While highly effective in protecting fish from columnaris, l-rhamnose is prohibitively expensive, underscoring the need for alternative cost-effective sources of rhamnose for disease control. One such alternative may be microbially produced glycolipid compounds termed rhamnolipids (RLs), which feature abundant l-rhamnose moieties and are readily available from commercial sources. In the present study, we examined whether commercially available RLs (administered either by immersion or via feed) would function similarly to l-rhamnose in affording host protection against F. columnare. A four-week feeding trial with basal and RL top-coated diets (basal diet + RLs) was conducted in channel catfish fingerlings. Surprisingly, columnaris challenges revealed significantly lower survival following the 10 d challenge period in RL diet fed fish when compared with the basal treatment group (p < 0.001). In fish fed RLs, we observed a rapid and large-scale upregulation of RBL1a immediately after challenge combined with a suppression of mucin and lysozyme transcripts. Similarly, fish that were briefly pre-exposed to RLs by immersion and then challenged exhibited lower survival as compared to unexposed fish during a 4 d trial. In conclusion, RLs do not represent an alternative to rhamnose as an experimental treatment for protecting catfish from columnaris mortality. Further research is needed to find other affordable and efficacious alternative sources of l-rhamnose.
[Mh] Termos MeSH primário: Dieta/veterinária
Suscetibilidade a Doenças/veterinária
Doenças dos Peixes/imunologia
Infecções por Flavobacteriaceae/veterinária
Glicolipídeos/imunologia
Ictaluridae/imunologia
[Mh] Termos MeSH secundário: Administração Oral
Ração Animal/análise
Animais
Suscetibilidade a Doenças/imunologia
Suscetibilidade a Doenças/microbiologia
Doenças dos Peixes/microbiologia
Infecções por Flavobacteriaceae/imunologia
Infecções por Flavobacteriaceae/microbiologia
Flavobacterium/fisiologia
Glicolipídeos/administração & dosagem
Ictaluridae/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glycolipids); 0 (rhamnolipid)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170404
[Lr] Data última revisão:
170404
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161108
[St] Status:MEDLINE


  10 / 1268 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27806254
[Au] Autor:Gao S; Liu S; Yao J; Zhou T; Li N; Li Q; Dunham R; Liu Z
[Ad] Endereço:The Fish Molecular Genetics and Biotechnology Laboratory, Aquatic Genomics Unit, School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL 36849, USA.
[Ti] Título:Taste receptors and gustatory associated G proteins in channel catfish, Ictalurus punctatus.
[So] Source:Comp Biochem Physiol Part D Genomics Proteomics;21:1-9, 2017 Mar.
[Is] ISSN:1878-0407
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Taste sensation plays a pivotal role in nutrient identification and acquisition. This is particularly true for channel catfish (Ictalurus punctatus) that live in turbid waters with limited visibility. This biological process is mainly mediated by taste receptors expressed in taste buds that are distributed in several organs and tissues, including the barbels and skin. In the present study, we identified a complete repertoire of taste receptor and gustatory associated G protein genes in the channel catfish genome. A total of eight taste receptor genes were identified, including five type I and three type II taste receptor genes. Their genomic locations, phylogenetic relations, orthologies and expression were determined. Phylogenetic and collinear analyses provided understanding of the evolution dynamics of this gene family. Furthermore, the motif and dN/dS analyses indicated that selection pressures of different degrees were imposed on these receptors. Additionally, four genes of gustatory associated G proteins were also identified. It was indicated that expression patterns of catfish taste receptors and gustatory associated G proteins across organs mirror the distribution of taste buds across organs. Finally, the expression comparison between catfish and zebrafish organs provided evidence of potential roles of catfish skin and gill involved in taste sensation.
[Mh] Termos MeSH primário: Proteínas de Peixes/genética
Ictaluridae/genética
Receptores Acoplados a Proteínas-G/genética
Papilas Gustativas/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Sequenciamento de Nucleotídeos em Larga Escala/métodos
Ictaluridae/crescimento & desenvolvimento
Filogenia
RNA Mensageiro/genética
Reação em Cadeia da Polimerase em Tempo Real
Reação em Cadeia da Polimerase Via Transcriptase Reversa
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fish Proteins); 0 (RNA, Messenger); 0 (Receptors, G-Protein-Coupled); 0 (taste receptors, type 1); 0 (taste receptors, type 2)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170901
[Lr] Data última revisão:
170901
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161103
[St] Status:MEDLINE



página 1 de 127 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde