Base de dados : MEDLINE
Pesquisa : B01.050.150.900.493.200.244.248 [Categoria DeCS]
Referências encontradas : 6625 [refinar]
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[PMID]:29175396
[Au] Autor:Wang F; Liu F; Chen W; Xu R; Wang W
[Ad] Endereço:School of Biological Science, Luoyang Normal University, Luoyang, 471022, China; Cold Water Fish Breeding Engineering Technology Research Center of Henan Province, Luoyang, 471022, China. Electronic address: wangfan7677@163.com.
[Ti] Título:Effects of triclosan (TCS) on hormonal balance and genes of hypothalamus-pituitary- gonad axis of juvenile male Yellow River carp (Cyprinus carpio).
[So] Source:Chemosphere;193:695-701, 2018 Feb.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Triclosan (TCS) is a broad spectrum antimicrobial agent which has been widely dispersed and determinated in the aquatic environment. However, the effects of TCS on reproductive endocrine in male fish are poorly understood. In this study, male Yellow River carp (Cyprinus carpio) were exposed to 0, 1/5, 1/10 and 1/20 LC (96 h LC of TCS to carp) TCS under semi-static conditions for 42 d. Vitellogenin (Vtg), 17ß-estradiol (E ), testosterone(T), gonadotropin (GtH), and gonadotropin-releasing hormone (GnRH) levels were measured by enzyme-linked immunosorbent assay (ELISA). Meanwhile, we also examined the mRNA expressions of aromatase, GtHs-ß, GnRH, estrogen receptor (Er), and androgen receptor (Ar) by quantitative Real-time Polymerase Chain Reaction (qRT-PCR). TCS induced Vtg levels of hepatopancreas, E levels of serum, and inhibited Ar and Er mRNA levels, suggesting that the induction of Vtg production by TCS was indirectly caused by non-Er pathways. TCS-induced Vtg levels by interfering with the reproductive axis at plenty of latent loci of male carps: (a) TCS exposure increased the aromatase mRNA expression of hypothalamus and gonad aromatase, consequently increasing serum concentrations of E to induce Vtg in hepatopancreas; (b) TCS treatment changed GtH-ß and GnRH mRNA expression and secretion, causing the disturbance of reproductive endocrine; (c) TCS exposure decreased Ar mRNA levels, indicating potential Ar-mediated antiandrogen action. These mechanisms showed that TCS may induce Vtg production in male carp by non-Er-mediated pathways.
[Mh] Termos MeSH primário: Carpas/metabolismo
Triclosan/toxicidade
Poluentes Químicos da Água/toxicidade
[Mh] Termos MeSH secundário: Animais
Anti-Infecciosos/toxicidade
Aromatase/genética
Sistema Endócrino/efeitos dos fármacos
Ensaio de Imunoadsorção Enzimática
Estradiol/análise
Hormônio Liberador de Gonadotropina/análise
Hormônio Liberador de Gonadotropina/genética
Gônadas/enzimologia
Gônadas/metabolismo
Hepatopâncreas/metabolismo
Hormônios/metabolismo
Hipotálamo/metabolismo
Masculino
Hipófise/metabolismo
RNA Mensageiro/análise
Reação em Cadeia da Polimerase em Tempo Real
Receptores Estrogênicos/genética
Reprodução/efeitos dos fármacos
Testosterona/análise
Vitelogeninas/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Hormones); 0 (RNA, Messenger); 0 (Receptors, Estrogen); 0 (Vitellogenins); 0 (Water Pollutants, Chemical); 33515-09-2 (Gonadotropin-Releasing Hormone); 3XMK78S47O (Testosterone); 4NM5039Y5X (Triclosan); 4TI98Z838E (Estradiol); EC 1.14.14.1 (Aromatase); EC 1.14.14.1 (CYP19A1 protein, human)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE


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[PMID]:28841529
[Au] Autor:Ghelichpour M; Taheri Mirghaed A; Mirzargar SS; Joshaghani H; Ebrahimzadeh Mousavi H
[Ad] Endereço:Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
[Ti] Título:Modification of saltwater stress response in Cyprinus carpio (Linnaeus, 1758) pre-exposed to pesticide indoxacarb.
[So] Source:Ecotoxicol Environ Saf;147:139-143, 2018 Jan.
[Is] ISSN:1090-2414
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:To evaluate the effects of indoxacarb on saltwater stress response in Cyprinus carpio, the fish were pre-exposed to indoxacarb (0, 0.75, 1.5 and 3mg/L denoted as CP, 0.75IT, 1.5IT and 3IT, respectively) for 21 days and then released to saltwater. A negative control (CN) group was included (the fish were held in indoxacarb-free water for the entire experiment). The fish were sampled immediately (0h) and 24, 48 and 72h after the salinity exposure for the analysis of plasma cortisol, glucose and sodium, chloride, potassium and calcium levels. All fish pre-exposed to 3mg/L indoxacarb, died after the first day of salinity challenge. CP showed typical cortisol response after the salinity challenge, but, cortisol response of the fish pre-exposed to indoxacarb (0.75IT and 1.5IT) was blocked. Plasma glucose increased significantly in all groups compared to the CN; however, this elevation had no consistent trend in 0.75IT and 1.5IT which indicated interference in glucose response due to indoxacarb exposure. Plasma sodium increased (compared to CN) in all groups after the salinity challenge. However, elevation in plasma chloride and potassium was significantly different among the groups and the indoxacarb-treated fish showed slightly sooner ionic disturbance. The results clearly indicate that indoxacarb impairs stress response of C. carpio and the fish may not be able to respond normally to additional stressors, which threatens their survival.
[Mh] Termos MeSH primário: Carpas/metabolismo
Hidrocortisona/sangue
Osmorregulação/efeitos dos fármacos
Oxazinas/toxicidade
Praguicidas/toxicidade
Poluentes Químicos da Água/toxicidade
[Mh] Termos MeSH secundário: Animais
Carpas/sangue
Cloretos/sangue
Relação Dose-Resposta a Droga
Potássio/sangue
Salinidade
Sódio/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chlorides); 0 (Oxazines); 0 (Pesticides); 0 (Water Pollutants, Chemical); 52H0D26MWR (indoxacarb); 9NEZ333N27 (Sodium); RWP5GA015D (Potassium); WI4X0X7BPJ (Hydrocortisone)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170826
[St] Status:MEDLINE


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[PMID]:28841525
[Au] Autor:Chupani L; Niksirat H; Velísek J; Stará A; Hradilová S; Kolarík J; Panácek A; Zusková E
[Ad] Endereço:University of South Bohemia in Ceské Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Centre of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Vodnany, Czech Republic. Electronic address: lchupani@frov.jcu.cz.
[Ti] Título:Chronic dietary toxicity of zinc oxide nanoparticles in common carp (Cyprinus carpio L.): Tissue accumulation and physiological responses.
[So] Source:Ecotoxicol Environ Saf;147:110-116, 2018 Jan.
[Is] ISSN:1090-2414
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Concerns regarding the potential toxic effects of zinc oxide nanoparticles (ZnO NPs) on aquatic organisms are growing due to the fact that NPs may be released into aquatic ecosystems. This study aimed to investigate the effects of dietary exposure to ZnO NPs on juvenile common carp (Cyprinus carpio). Fish were fed a spiked diets at doses 50 and 500mg of ZnO NPs per kg of feed for 6 weeks followed by a 2-week recovery period. Fish were sampled every 2 weeks for haematology trends, blood biochemistry measures, histology analyses, and determination of the accumulation of zinc in tissues. At the end of the exposure and post-exposure periods, fish were sampled for an assessment of lipid peroxidation levels. Dietborne ZnO NPs had no effects on haematology, blood biochemistry, and lipid peroxidation levels during the exposure period. After the recovery period, aspartate aminotransferase activity significantly (p < 0.05) increased and alanine transferase activity significantly (p < 0.05) decreased in the higher exposure group. The level of lipid peroxidation significantly (p < 0.05) decreased in liver of treated fish after 2 weeks post-exposure period. A histological examination revealed mild histopathological changes in kidneys during exposure. Our results did not show a significant increase of zinc content at the end of experiment in any of tested organs. However, chronic dietary exposure to ZnO NPs might affect kidney and liver function.
[Mh] Termos MeSH primário: Carpas/fisiologia
Monitoramento Ambiental/métodos
Nanopartículas Metálicas/toxicidade
Estresse Oxidativo/efeitos dos fármacos
Poluentes Químicos da Água/toxicidade
Óxido de Zinco/toxicidade
[Mh] Termos MeSH secundário: Animais
Carpas/metabolismo
Dieta
Relação Dose-Resposta a Droga
Rim/efeitos dos fármacos
Rim/metabolismo
Peroxidação de Lipídeos/efeitos dos fármacos
Fígado/efeitos dos fármacos
Fígado/metabolismo
Fatores de Tempo
Distribuição Tecidual
Poluentes Químicos da Água/metabolismo
Óxido de Zinco/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Water Pollutants, Chemical); SOI2LOH54Z (Zinc Oxide)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170826
[St] Status:MEDLINE


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[PMID]:28450256
[Au] Autor:Dietrich MA; Irnazarow I; Ciereszko A
[Ad] Endereço:Department of Gametes and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-748 Olsztyn, Poland. Electronic address: m.dietrich@pan.olsztyn.pl.
[Ti] Título:Proteomic identification of seminal plasma proteins related to the freezability of carp semen.
[So] Source:J Proteomics;162:52-61, 2017 Jun 06.
[Is] ISSN:1876-7737
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The variation in sperm freezability among individuals within a fish species is a major factor justifying the identification of useful predictive indicators of cryopreservation success. It is unknown at present whether the protein composition of fish seminal plasma affects sperm freezability. Therefore, the aims of this study were to compare the proteome of carp seminal plasma from semen rated as good (GF) and poor (PF) freezability by two-dimensional difference gel electrophoresis followed by MALDI-TOF/TOF mass spectrometry. The semen was classified as GF and PF based on sperm motility assessment after freeze/thawing. Five spots representing three proteins were more abundant in GF, while ten spots representing seven proteins were more abundant in PF seminal plasma. The majority of proteins present in higher abundance in PF seminal plasma were associated with the innate immune response. On the other hand, higher freezability was associated with proteins involved in the maintenance of sperm membrane integrity and antioxidative protection. These results indicate that carp semen freezability levels may be related to different seminal plasma protein profiles. Lower usefulness of spermatozoa in cryopreservation may be related to previous infection or stress leading to sublethal changes to sperm structure. SIGNIFICANCE: Sperm quality parameters such as motility, viability and sperm concentration have been used as predictive tools of sperm cryopreservation potential in fish species However, the usefulness of initial motility parameters as indicators of freezability varies among fish species and between individuals within a species. Recent studies in mammals revealed that male-to-male variability in cryoresistance can be attributed to differences in seminal plasma protein composition. To the best of our knowledge, no proteomic studies linking the protein composition of fish seminal plasma and freezing resilience have been performed in fish. Our results indicate for the first time that factors regulating how carp semen tolerate cryopreservation may be related to the different protein profiles of carp seminal plasma. The obtained results provide new insight into understanding the molecular mechanisms underlying cryoresistance of carp semen and provide a tool for the improvement of a long-term sperm preservation procedure.
[Mh] Termos MeSH primário: Carpas
Congelamento
Sêmen/química
Proteínas de Plasma Seminal/análise
[Mh] Termos MeSH secundário: Animais
Criopreservação
Masculino
Proteômica/métodos
Sêmen/citologia
Espermatozoides/citologia
Espectrometria de Massas em Tandem
Eletroforese em Gel Diferencial Bidimensional
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Seminal Plasma Proteins)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE


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[PMID]:29220445
[Au] Autor:Chen Y; Shi M; Zhang W; Cheng Y; Wang Y; Xia XQ
[Ad] Endereço:Institute of Hydrobiology, The Chinese Academy of Sciences, Wuhan 430072, China.
[Ti] Título:The Grass Carp Genome Database (GCGD): an online platform for genome features and annotations.
[So] Source:Database (Oxford);2017, 2017 Jan 01.
[Is] ISSN:1758-0463
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Database URL: http://bioinfo.ihb.ac.cn/gcgd.
[Mh] Termos MeSH primário: Carpas/genética
Bases de Dados Genéticas
Genoma/genética
Anotação de Sequência Molecular/métodos
[Mh] Termos MeSH secundário: Animais
Repetições de Microssatélites/genética
Alinhamento de Sequência
Transcriptoma/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171209
[St] Status:MEDLINE
[do] DOI:10.1093/database/bax051


  6 / 6625 MEDLINE  
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[PMID]:29256279
[Au] Autor:Herczeg D; Sipos D; Dán Á; Loy C; Kallert DM; Eszterbauer E
[Ad] Endereço:1 Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences , Hungária krt. 21, H-1143 Budapest , Hungary.
[Ti] Título:The effect of dietary immunostimulants on the susceptibility of common carp (Cyprinus carpio) to the white spot parasite, Ichthyophthirius multifiliis.
[So] Source:Acta Vet Hung;65(4):517-530, 2017 12.
[Is] ISSN:0236-6290
[Cp] País de publicação:Hungary
[La] Idioma:eng
[Ab] Resumo:One of the main obstacles in freshwater aquaculture is the parasitic ciliate Ichthyophthirius multifiliis (Ich), the causative agent of white spot disease. The use of immunostimulants as feed additives may be a promising approach to control Ich infection. In the present study, we tested the prophylactic effect of orally administered ß-1,3/1,6-glucan and propolis extract E50 against Ich infection in common carp. In total, 122 fish were separated into three experimental groups fed with a control, 3% ß-glucan and 1% propolis diet for 40 consecutive days, respectively. On day 40, 16 fish per group were individually exposed to Ich theronts and the number of trophonts was counted 5 days post exposure. Relative gene expression of interleukin 1-ß (IL-1-ß) in common carp liver was examined by qPCR. Compared to control, the mean infection intensity was lower in the ß-glucan- and propolis-fed groups; however, the difference was not statistically significant. The relative expression of IL-1-ß significantly decreased in the propolis-fed group at day 10. In the ß-glucan-fed group, a significant IL-1-ß decrease was detected at day 15 compared to control. Although the Ich infection intensity was slightly decreased in both treated groups, and IL-1-ß was moderately down-regulated in the liver of common carp, our results suggest that the applied feeding regime is insufficient to prevent Ich outbreaks in common carp.
[Mh] Termos MeSH primário: Adjuvantes Imunológicos/administração & dosagem
Carpas
Infecções por Cilióforos/veterinária
Cilióforos
Suplementos Nutricionais
Doenças dos Peixes/parasitologia
[Mh] Termos MeSH secundário: Animais
Cilióforos/genética
Cilióforos/isolamento & purificação
Infecções por Cilióforos/parasitologia
Infecções por Cilióforos/prevenção & controle
RNA/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Adjuvants, Immunologic); 63231-63-0 (RNA)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171220
[St] Status:MEDLINE
[do] DOI:10.1556/004.2017.050


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[PMID]:29381694
[Au] Autor:Schultz MT
[Ad] Endereço:Environmental Laboratory, Engineer Research and Development Center, United States Army Corps of Engineers, Vicksburg, Mississippi, United States of America.
[Ti] Título:Inference of genetic marker concentrations from field surveys to detect environmental DNA using Bayesian updating.
[So] Source:PLoS One;13(1):e0190603, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Field studies to detect environmental DNA (eDNA) can be undertaken to infer the presence of a rare or cryptic species in a water body. These studies are implemented by collecting water samples from the water body, processing those samples to isolate genetic material contained in the water sample, and using a laboratory assay to find a species-specific genetic marker within a sample of the genetic material. To date, conventional polymerase chain reaction (PCR) has been one of the most widely used assays in field studies to detect eDNA. This assay is strictly a test for the presence of the genetic marker. It provides no estimate of the concentration of the target genetic marker in the sample or in the environment. Understanding the concentration of a target marker in the environment is a critical first step toward using the results of eDNA field surveys to support inferences about the location and strength of eDNA sources. In this study, the results of eDNA field surveys are combined with a model of the sensitivity of the field survey methods to estimate target marker concentrations using Bayesian updating. The method is demonstrated for Asian carp in the Chicago Area Waterway System (CAWS) using the results of field surveys for eDNA carried out during the period 2009 through 2012, a four-year period during which more than 5,800 two-liter water samples were collected and analyzed using PCR. Concentrations of bighead carp (Hypophthalmichthys nobilis) and silver carp (Hypophthalmichthys molitrix) eDNA are estimated for twenty hydrologic reaches of the CAWS. This study also assesses the sensitivity of these concentration estimates to evidentiary criteria that limit what evidence is used in Bayesian updating based on requirements for sampling intensity and frequency.
[Mh] Termos MeSH primário: Teorema de Bayes
Carpas/genética
DNA/isolamento & purificação
Marcadores Genéticos
[Mh] Termos MeSH secundário: Animais
Chicago
Ecossistema
Monitoramento Ambiental/métodos
Espécies Introduzidas
Inquéritos e Questionários
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Genetic Markers); 9007-49-2 (DNA)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180131
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190603


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[PMID]:29247645
[Au] Autor:Qin C; Yang L; Zheng W; Yan X; Lu R; Xie D; Nie G
[Ad] Endereço:Engineering Technology Research Center of Henan Province for Aquatic Animal Cultivation, College of Fisheries, Henan Normal University, No. 46 Jianshe Road, Xinxiang 453007, PR China.
[Ti] Título:Effects of dietary glucose and sodium chloride on intestinal glucose absorption of common carp (Cyprinus carpio L.).
[So] Source:Biochem Biophys Res Commun;495(2):1948-1955, 2018 01 08.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The co-transport of sodium and glucose is the first step for intestinal glucose absorption. Dietary glucose and sodium chloride (NaCl) may facilitate this physiological process in common carp (Cyprinus carpio L.). To test this hypothesis, we first investigated the feeding rhythm of intestinal glucose absorption. Carps were fed to satiety once a day (09:00 a.m.) for 1 month. Intestinal samples were collected at 01:00, 05:00, 09:00, 13:00, 17:00 and 21:00. Result showed that food intake greatly enhanced sodium/glucose cotransporter 1 (SGLT1) and glucose transporter type 2 (GLUT2) expressions, and improved glucose absorption, with highest levels at 09:00 a.m.. Then we designed iso-nitrogenous and iso-energetic diets with graded levels of glucose (10%, 20%, 30%, 40% and 50%) and NaCl (0%, 1%, 3% and 5%), and submitted to feeding trial for 10 weeks. The expressions of SGLT1 and GLUT2, brush border membrane vesicles (BBMVs) glucose transport and intestinal villus height were determined after the feeding trial. Increasing levels of dietary glucose and NaCl up-regulated mRNA and protein levels of SGLT1 and GLUT2, enhanced BBMVs glucose transport in the proximal, mid and distal intestine. As for histological adaptive response, however, high-glucose diet prolonged while high-NaCl diet shrank intestinal villus height. Furthermore, we also found that higher mRNA levels of SGLT1 and GLUT2, higher glucose transport capacity of BBMVs, and higher intestinal villus were detected in the proximal and mid intestine, compared to the distal part. Taken together, our study indicated that intestinal glucose absorption in carp was primarily occurred in the proximal and mid intestine, and increasing levels of dietary glucose and NaCl enhanced intestinal glucose absorption in carp.
[Mh] Termos MeSH primário: Carpas/metabolismo
Açúcares da Dieta/metabolismo
Ingestão de Alimentos/fisiologia
Glucose/metabolismo
Absorção Intestinal/fisiologia
Cloreto de Sódio na Dieta/metabolismo
[Mh] Termos MeSH secundário: Animais
Taxa de Depuração Metabólica
Distribuição Tecidual
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Dietary Sugars); 0 (Sodium Chloride, Dietary); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171217
[St] Status:MEDLINE


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[PMID]:29360831
[Au] Autor:Knytl M; Kalous L; Rylková K; Choleva L; Merilä J; Ráb P
[Ad] Endereço:Department of Cell Biology, Faculty of Science, Charles University, Prague, Czech Republic.
[Ti] Título:Morphologically indistinguishable hybrid Carassius female with 156 chromosomes: A threat for the threatened crucian carp, C. carassius, L.
[So] Source:PLoS One;13(1):e0190924, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The crucian carp Carassius carassius (Linnaeus, 1758), is native to many European freshwaters. Despite its wide distribution, the crucian carp is declining in both the number and sizes of populations across much of its range. Here we studied 30 individuals of a putative pure population from Helsinki, Finland. Despite clear external morphological features of C. carassius, an individual was of a higher ploidy level than the others. We therefore applied a set of molecular genetic (S7 nuclear and cytochrome b mitochondrial genes) and cytogenetic tools (sequential fluorescent 4', 6-diamidino-2-phenylindole [DAPI], Chromomycin A3 [CMA3], C-banding and in situ hybridization [FISH] with both 5S and 28S ribosomal DNA probes) to determine its origin. While all examined characteristics of a diploid representative male (CCAHe2Fi) clearly corresponded to those of C. carassius, a triploid individual (CCAHe1Fi) was more complex. Phylogenetic analysis revealed that the nuclear genome of CCAHe1Fi contained three haploid sets: two C. gibelio and one C. carassius. However the mitochondrial DNA was that of C. gibelio, demonstrating its hybrid origin. The FISH revealed three strong (more intensive) 5S rDNA loci, confirming the triploid status, and an additional 24 weak (less intensive) signals were observed in the chromosome complement of CCAHe1Fi. On the other hand, only two strong and 16 weak 5S rDNA signals were visible on the chromosomes of the CCAHe2Fi male. 28S rDNA FISH revealed four strong signals in both CCAHe1Fi and CCAHe2Fi individuals. CMA3 staining revealed four to six CMA3-positive bands of CCAHe1Fi, while that of diploids contained only two to four. The fact that a polyploid hybrid Carassius female with a strong invasive potential may share morphological characters typical for endangered C. carassius highlights a need to combine genetic investigations of Carassius cryptic diversity with conservation measures of C. carassius in Europe.
[Mh] Termos MeSH primário: Carpas/anatomia & histologia
Carpas/genética
Triploidia
[Mh] Termos MeSH secundário: Animais
Carpas/classificação
Bandeamento Cromossômico
Diploide
Espécies em Perigo de Extinção
Europa (Continente)
Feminino
Finlândia
Marcadores Genéticos
Variação Genética
Hibridização Genética
Hibridização in Situ Fluorescente
Cariótipo
Masculino
Filogenia
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Genetic Markers)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180206
[Lr] Data última revisão:
180206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180124
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190924


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[PMID]:28466816
[Au] Autor:Mise Yonar S; Yonar ME; Ural MS
[Ad] Endereço:Department of Aquaculture and Fish Diseases, Firat University, Fisheries Faculty, Elazig, Turkey.
[Ti] Título:Antioxidant effect of curcumin against exposure to malathion in Cyprinus carpio.
[So] Source:Cell Mol Biol (Noisy-le-grand);63(3):68-72, 2017 Mar 31.
[Is] ISSN:1165-158X
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to determine protective effects of curcumin on oxidant/antioxidant parameters in Cyprinus carpio exposed to malathion. The fish were exposed to two sublethal concentrations of malathion (0.5 and 1 mg/L), and curcumin (100 mg per kg of fish weight) was simultaneously administered for 14 days. Malondialdehyde level and superoxide dismutase, catalase, glutathione peroxidase, and glutathione-S-transferase  activities were monitored in liver, kidney and gills, which were collected at the end of the experiment. The results revealed a significant increase in the malondialdehyde levels of the groups that were exposed to malathion. Also, malathion exposure caused a significant increase in superoxide dismutase, catalase, and glutathione-S-transferase  activities and a significant decrease in glutathione peroxidase activity. Treatment with curcumin attenuated the malathion-induced oxidative stress by significantly decreasing the levels of malondialdehyde in the tissues. In addition, curcumin reversed the superoxide dismutase, catalase, glutathione peroxidase, and glutathione-S-transferase activities. In conclusion, this study demonstrated that malathion caused oxidative stress and negative alterations on the antioxidant enzyme activities of the fish. However, this toxic effect was neutralised by the administration of curcumin.
[Mh] Termos MeSH primário: Antioxidantes/farmacologia
Carpas/metabolismo
Curcumina/farmacologia
Malation/toxicidade
[Mh] Termos MeSH secundário: Animais
Comportamento Animal/efeitos dos fármacos
Catalase/metabolismo
Glutationa Peroxidase/metabolismo
Glutationa Transferase/metabolismo
Malondialdeído/metabolismo
Estresse Oxidativo/efeitos dos fármacos
Superóxido Dismutase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 4Y8F71G49Q (Malondialdehyde); EC 1.11.1.6 (Catalase); EC 1.11.1.9 (Glutathione Peroxidase); EC 1.15.1.1 (Superoxide Dismutase); EC 2.5.1.18 (Glutathione Transferase); IT942ZTH98 (Curcumin); U5N7SU872W (Malathion)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180115
[Lr] Data última revisão:
180115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.14715/cmb/2017.63.3.13



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