Base de dados : MEDLINE
Pesquisa : B01.050.150.900.493.370.853.392 [Categoria DeCS]
Referências encontradas : 1230 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 123 ir para página                         

  1 / 1230 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28214875
[Au] Autor:Pose-Méndez S; Rodríguez-Moldes I; Candal E; Mazan S; Anadón R
[Ad] Endereço:Departamento de Bioloxía Funcional, Centro de Investigación en Bioloxía (CIBUS), Universidade de Santiago de Compostela, Santiago de Compostela, Spain.
[Ti] Título:A Developmental Study of the Cerebellar Nucleus in the Catshark, a Basal Gnathostome.
[So] Source:Brain Behav Evol;89(1):1-14, 2017.
[Is] ISSN:1421-9743
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The output of the cerebellar cortex is mainly released via cerebellar nuclei which vary in number and complexity among gnathostomes, extant vertebrates with a cerebellum. Cartilaginous fishes, a basal gnathostome lineage, show a conspicuous, well-organized cerebellar nucleus, unlike ray-finned fishes. To gain insight into the evolution and development of the cerebellar nucleus, we analyzed in the shark Scyliorhinus canicula (a chondrichthyan model species) the developmental expression of several genes coding for transcription factors (ScLhx5,ScLhx9,ScTbr1, and ScEn2) and the distribution of the protein calbindin, since all appear to be involved in cerebellar nuclei patterning in other gnathostomes. Three regions (subventricular, medial or central, and lateral or superficial) became recognizable in the cerebellar nucleus of this shark during development. Present genoarchitectonic and neurochemical data in embryos provide insight into the origin of the cerebellar nucleus in chondrichthyans and support a tripartite mediolateral organization of the cerebellar nucleus, as previously described in adult sharks. Furthermore, the expression pattern of ScLhx5,ScLhx9, and ScTbr1 in this shark, together with that of markers of proliferation, migration, and early differentiation of neurons, is compatible with the hypothesis that, as in mammals, different subsets of cerebellar nucleus neurons are originated from progenitors of 2 different sources: the ventricular zone of the cerebellar plate and the rhombic lip. We also present suggestive evidence that Lhx9 expression is involved in cerebellar nuclei patterning early on in gnathostome evolution, rather than representing an evolutionary innovation of the dentate nucleus in mammals, as previously hypothesized.
[Mh] Termos MeSH primário: Evolução Biológica
Calbindinas/metabolismo
Núcleos Cerebelares
Cação (Peixe)
Proteínas de Peixes/metabolismo
Regulação da Expressão Gênica no Desenvolvimento/fisiologia
[Mh] Termos MeSH secundário: Animais
Calbindinas/genética
Núcleos Cerebelares/embriologia
Núcleos Cerebelares/metabolismo
Cação (Peixe)/embriologia
Cação (Peixe)/genética
Cação (Peixe)/metabolismo
Proteínas de Peixes/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Calbindins); 0 (Fish Proteins)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170719
[Lr] Data última revisão:
170719
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170220
[St] Status:MEDLINE
[do] DOI:10.1159/000453654


  2 / 1230 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:27802150
[Au] Autor:Wood CM; Giacomin M
[Ad] Endereço:Bamfield Marine Sciences Centre, Bamfield, BC, Canada V0R 1B0 woodcm@zoology.ubc.ca giacomin@zoology.ubc.ca.
[Ti] Título:Feeding through your gills and turning a toxicant into a resource: how the dogfish shark scavenges ammonia from its environment.
[So] Source:J Exp Biol;219(Pt 20):3218-3226, 2016 Oct 15.
[Is] ISSN:1477-9145
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Nitrogen (N) appears to be a limiting dietary resource for elasmobranchs, required not only for protein growth but also for urea-based osmoregulation. Building on recent evidence that the toxicant ammonia can be taken up actively at the gills of the shark and made into the valuable osmolyte urea, we demonstrate that the uptake exhibits classic Michaelis-Menten saturation kinetics with an affinity constant (K ) of 379 µmol l , resulting in net N retention at environmentally realistic ammonia concentrations (100-400 µmol l ) and net N loss through stimulated urea-N excretion at higher levels. Ammonia-N uptake rate increased or decreased with alterations in seawater pH, but the changes were much less than predicted by the associated changes in seawater P , and more closely paralleled changes in seawater NH concentration. Ammonia-N uptake rate was insensitive to amiloride (0.1 mmol l ) or to a 10-fold elevation in seawater K concentration (to 100 mmol l ), suggesting that the mechanism does not directly involve Na or K transporters, but was inhibited by blockade of glutamine synthetase, the enzyme that traps ammonia-N to fuel the ornithine-urea cycle. High seawater ammonia inhibited uptake of the ammonia analogue [ C]methylamine. The results suggest that branchial ammonia-N uptake may significantly supplement dietary N intake, amounting to about 31% of the nitrogen acquired from the diet. They further indicate the involvement of Rh glycoproteins (ammonia channels), which are expressed in dogfish gills, in normal ammonia-N uptake and retention.
[Mh] Termos MeSH primário: Amônia/metabolismo
Cação (Peixe)/fisiologia
Meio Ambiente
Brânquias/fisiologia
[Mh] Termos MeSH secundário: Amilorida/farmacologia
Animais
Radioisótopos de Carbono
Brânquias/efeitos dos fármacos
Concentração de Íons de Hidrogênio
Masculino
Metionina Sulfoximina/farmacologia
Metilaminas/metabolismo
Nitrogênio/metabolismo
Potássio/análise
Água do Mar/química
Ureia/metabolismo
Água/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carbon Radioisotopes); 0 (Methylamines); 059QF0KO0R (Water); 1982-67-8 (Methionine Sulfoximine); 7664-41-7 (Ammonia); 7DZO8EB0Z3 (Amiloride); 8W8T17847W (Urea); BSF23SJ79E (methylamine); N762921K75 (Nitrogen); RWP5GA015D (Potassium)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170809
[Lr] Data última revisão:
170809
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161102
[St] Status:MEDLINE


  3 / 1230 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27653983
[Au] Autor:Telles CJ; Decker SE; Motley WW; Peters AW; Mehr AP; Frizzell RA; Forrest JN
[Ad] Endereço:Nephrology Division, Department of Medicine, Yale University School of Medicine, New Haven, Connecticut.
[Ti] Título:Functional and molecular identification of a TASK-1 potassium channel regulating chloride secretion through CFTR channels in the shark rectal gland: implications for cystic fibrosis.
[So] Source:Am J Physiol Cell Physiol;311(6):C884-C894, 2016 Dec 01.
[Is] ISSN:1522-1563
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In the shark rectal gland (SRG), apical chloride secretion through CFTR channels is electrically coupled to a basolateral K conductance whose type and molecular identity are unknown. We performed studies in the perfused SRG with 17 K channel inhibitors to begin this search. Maximal chloride secretion was markedly inhibited by low-perfusate pH, bupivicaine, anandamide, zinc, quinidine, and quinine, consistent with the properties of an acid-sensitive, four-transmembrane, two-pore-domain K channel (4TM-K2P). Using PCR with degenerate primers to this family, we identified a TASK-1 fragment in shark rectal gland, brain, gill, and kidney. Using 5' and 3' rapid amplification of cDNA ends PCR and genomic walking, we cloned the full-length shark gene (1,282 bp), whose open reading frame encodes a protein of 375 amino acids that was 80% identical to the human TASK-1 protein. We expressed shark and human TASK-1 cRNA in Xenopus oocytes and characterized these channels using two-electrode voltage clamping. Both channels had identical current-voltage relationships (outward rectifying) and a reversal potential of -90 mV. Both were inhibited by quinine, bupivicaine, and acidic pH. The pKa for current inhibition was 7.75 for shark TASK-1 vs. 7.37 for human TASK-1, values similar to the arterial pH for each species. We identified this protein in SRG by Western blot and confocal immunofluorescent microscopy and detected the protein in SRG and human airway cells. Shark TASK-1 is the major K channel coupled to chloride secretion in the SRG, is the oldest 4TM 2P family member identified, and is the first TASK-1 channel identified to play a role in setting the driving force for chloride secretion in epithelia. The detection of this potassium channel in mammalian lung tissue has implications for human biology and disease.
[Mh] Termos MeSH primário: Cloretos/metabolismo
Regulador de Condutância Transmembrana em Fibrose Cística/metabolismo
Fibrose Cística/metabolismo
Proteínas do Tecido Nervoso/metabolismo
Canais de Potássio de Domínios Poros em Tandem/metabolismo
Canais de Potássio/metabolismo
Glândula de Sal/metabolismo
Tubarões/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
DNA Complementar/genética
Cação (Peixe)/metabolismo
Seres Humanos
Proteínas do Tecido Nervoso/genética
Oócitos/metabolismo
Canais de Potássio de Domínios Poros em Tandem/genética
Xenopus laevis/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chlorides); 0 (DNA, Complementary); 0 (Nerve Tissue Proteins); 0 (Potassium Channels); 0 (Potassium Channels, Tandem Pore Domain); 0 (potassium channel subfamily K member 3); 126880-72-6 (Cystic Fibrosis Transmembrane Conductance Regulator)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170605
[Lr] Data última revisão:
170605
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160923
[St] Status:MEDLINE
[do] DOI:10.1152/ajpcell.00030.2016


  4 / 1230 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27606943
[Au] Autor:Finucci B; Bustamante C; Jones EG; Dunn MR
[Ad] Endereço:School of Biological Sciences, Victoria University of Wellington, P. O. Box 600, Wellington, 6140, New Zealand. brit.finucci@vuw.ac.nz.
[Ti] Título:Reproductive biology and feeding habits of the prickly dogfish Oxynotus bruniensis.
[So] Source:J Fish Biol;89(5):2326-2344, 2016 Nov.
[Is] ISSN:1095-8649
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The reproductive biology and diet of prickly dogfish Oxynotus bruniensis, a deep-sea elasmobranch, endemic to the outer continental and insular shelves of southern Australia and New Zealand, and caught as by-catch in demersal fisheries, are described from specimens caught in New Zealand waters. A total of 53 specimens were obtained from research surveys and commercial fisheries, including juveniles and adults ranging in size from 33·5 to 75·6 cm total length (L ). Estimated size-at-maturity was 54·7 cm L in males and 64·0 cm L in females. Three gravid females (65·0, 67·5 and 71·2 cm L ) were observed, all with eight embryos. Size-at-birth was estimated to be 25-27 cm L . Vitellogenesis was not concurrent with embryo development. Analysis of diet from stomach contents, including DNA identification of prey using the mitochondrial genes cox1 and nadh2, revealed that O. bruniensis preys exclusively on the egg capsules of holocephalans, potentially making it the only known elasmobranch with a diet reliant solely upon other chondrichthyans. Based on spatial overlap with deep-sea fisheries, a highly specialized diet, and reproductive characteristics representative of a low productivity fish, the commercial fisheries by-catch of O. bruniensis may put this species at relatively high risk of overfishing.
[Mh] Termos MeSH primário: Dieta
Cação (Peixe)/fisiologia
Reprodução
[Mh] Termos MeSH secundário: Animais
Comportamento Alimentar
Feminino
Pesqueiros
Conteúdo Gastrointestinal
Masculino
Nova Zelândia
Tubarões
Austrália do Sul
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160909
[St] Status:MEDLINE
[do] DOI:10.1111/jfb.13116


  5 / 1230 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27451157
[Au] Autor:Oliveira A; Gonzalez MH; Queiroz HM; Cadore S
[Ad] Endereço:Federal University of Paraná - Chemistry Department, P.O. Box 19032, 81531-980 Curitiba, PR, Brazil. Electronic address: aoliveira@quimica.ufpr.br.
[Ti] Título:Fractionation of inorganic arsenic by adjusting hydrogen ion concentration.
[So] Source:Food Chem;213:76-82, 2016 Dec 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The inorganic fraction of arsenic species, iAs=∑[As(III)+As(V)] present in fish samples can be quantified in the presence of other arsenic species also found in fishes, such as: monomethylarsonic acid (MMA), dimethylarsinic acid (DMA) and arsenobetaine (AsB). The toxic arsenic fraction was selected taking into account the dissociation constants of these arsenic species in different hydrogen ions concentration leading to the arsine formation from iAs compounds detected as As(III) by HG AAS. For thus, a microwave assisted extraction was carried out using HCl 1molL(-1) in order to maintain the integrity of the arsenic species in this mild extraction media. Recovery experiments were done for iAs fraction, in the presence of other arsenic species. The recovery values obtained for iAs fraction added were quantitative about 87-107% (for N=3, RSD⩽3%). The limit of detection (LOD), and the limit of quantification (LOQ), were 5µgkg(-1) and 16µgkg(-1) respectively.
[Mh] Termos MeSH primário: Arsênico/química
Arsenicais/química
[Mh] Termos MeSH secundário: Animais
Fracionamento Químico
Cromatografia
Cação (Peixe)
Peixes
Inocuidade dos Alimentos/métodos
Hidrogênio/química
Concentração de Íons de Hidrogênio
Limite de Detecção
Micro-Ondas
Músculos/metabolismo
Medição de Risco
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arsenicals); 7YNJ3PO35Z (Hydrogen); J37VJ5709S (monomethylarsonic acid); N712M78A8G (Arsenic); UWC1LS4V3I (arsenobetaine); V1I29R0RJQ (arsine)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160725
[St] Status:MEDLINE


  6 / 1230 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27357054
[Au] Autor:O'Harte FP; Ng MT; Lynch AM; Conlon JM; Flatt PR
[Ad] Endereço:School of Biomedical Sciences, Saad Centre for Pharmacy and Diabetes, University of Ulster, Coleraine, UK. fpm.oharte@ulster.ac.uk.
[Ti] Título:Dogfish glucagon analogues counter hyperglycaemia and enhance both insulin secretion and action in diet-induced obese diabetic mice.
[So] Source:Diabetes Obes Metab;18(10):1013-24, 2016 Oct.
[Is] ISSN:1463-1326
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:AIMS: To investigate the antidiabetic actions of three dogfish glucagon peptide analogues [known glucagon-like peptide-1 and glucagon receptor co-agonists] after chronic administration in diet-induced high-fat-diet-fed diabetic mice. MATERIALS AND METHODS: National Institutes of Health Swiss mice were pre-conditioned to a high-fat diet (45% fat) for 100 days, and control mice were fed a normal diet (10% fat). Normal diet control and high-fat-fed control mice received twice-daily intraperitoneal (i.p.) saline injections, while the high-fat-fed treatment groups (n = 8) received twice-daily injections of exendin-4(1-39), [S2a]dogfish glucagon, [S2a]dogfish glucagon exendin-4(31-39) or [S2a]dogfish glucagon-Lys(30) -γ-glutamyl-PAL (25 nmol/kg body weight) for 51 days. RESULTS: After dogfish glucagon analogue treatment, there was a rapid and sustained decrease in non-fasting blood glucose and an associated insulinotropic effect (analysis of variance, p < .05 to <.001) compared with saline-treated high-fat-fed controls. All peptide treatments significantly improved i.p. and oral glucose tolerance with concomitant increased insulin secretion compared with saline-treated high-fat-fed controls (p <.05 to <.001). After chronic treatment, no receptor desensitization was observed but insulin sensitivity was enhanced for all peptide-treated groups (p < .01 to <.001) except [S2a]dogfish glucagon. Both exendin-4 and [S2a]dogfish glucagon exendin-4(31-39) significantly reduced plasma triglyceride concentrations compared with those found in lean controls (p = .0105 and p = .0048, respectively). Pancreatic insulin content was not affected by peptide treatments but [S2a]dogfish glucagon and [S2a]dogfish glucagon exendin-4(31-39) decreased pancreatic glucagon by 28%-34% (p = .0221 and p = .0075, respectively). The percentage of ß-cell area within islets was increased by exendin-4 and peptide analogue treatment groups compared with high-fat-fed controls and the ß-cell area decreased (p < .05 to <.01). CONCLUSIONS: Overall, dogfish glucagon co-agonist analogues had several beneficial metabolic effects, showing therapeutic potential for type 2 diabetes.
[Mh] Termos MeSH primário: Diabetes Mellitus Experimental/metabolismo
Glucagon/farmacologia
Hiperglicemia/prevenção & controle
Insulina/fisiologia
Insulina/secreção
Obesidade/metabolismo
[Mh] Termos MeSH secundário: Animais
Glicemia/efeitos dos fármacos
Glicemia/metabolismo
Diabetes Mellitus Experimental/complicações
Dieta Hiperlipídica
Cação (Peixe)/metabolismo
Glucagon/análogos & derivados
Glucagon/metabolismo
Teste de Tolerância a Glucose
Hiperglicemia/complicações
Resistência à Insulina
Células Secretoras de Insulina/efeitos dos fármacos
Células Secretoras de Insulina/metabolismo
Células Secretoras de Insulina/patologia
Masculino
Camundongos
Camundongos Obesos
Obesidade/etiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Blood Glucose); 0 (Insulin); 9007-92-5 (Glucagon)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160701
[St] Status:MEDLINE
[do] DOI:10.1111/dom.12713


  7 / 1230 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27179756
[Au] Autor:O'Harte FP; Ng MT; Lynch AM; Conlon JM; Flatt PR
[Ad] Endereço:The Saad Centre for Pharmacy & Diabetes, School of Biomedical Sciences, University of Ulster, Coleraine, Co. Derry, BT52 1SA, Northern Ireland, UK. Electronic address: fpm.oharte@ulster.ac.uk.
[Ti] Título:Novel dual agonist peptide analogues derived from dogfish glucagon show promising in vitro insulin releasing actions and antihyperglycaemic activity in mice.
[So] Source:Mol Cell Endocrinol;431:133-44, 2016 Aug 15.
[Is] ISSN:1872-8057
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:The antidiabetic potential of thirteen novel dogfish glucagon derived analogues were assessed in vitro and in acute in vivo studies. Stable peptide analogues enhanced insulin secretion from BRIN-BD11 ß-cells (p < 0.001) and reduced acute glycaemic responses following intraperitoneal glucose (25 nmol/kg) in healthy NIH Swiss mice (p < 0.05-p<0.001). The in vitro insulinotropic actions of [S2a]dogfish glucagon, [S2a]dogfish glucagon-exendin-4(31-39) and [S2a]dogfish glucagon-Lys(30)-γ-glutamyl-PAL, were blocked (p < 0.05-p<0.001) by the specific GLP-1 and glucagon receptor antagonists, exendin-4(9-39) and (desHis(1)Pro(4)Glu(9))glucagon amide but not by (Pro(3))GIP, indicating lack of GIP receptor involvement. These analogues dose-dependently stimulated cAMP production in GLP-1 and glucagon (p < 0.05-p<0.001) but not GIP-receptor transfected cells. They improved acute glycaemic and insulinotropic responses in high-fat fed diabetic mice and in wild-type C57BL/6J and GIPR-KO mice (p < 0.05-p<0.001), but not GLP-1R-KO mice, confirming action on GLP-1 but not GIP receptors. Overall, dogfish glucagon analogues have potential for diabetes therapy, exerting beneficial metabolic effects via GLP-1 and glucagon receptors.
[Mh] Termos MeSH primário: Cação (Peixe)/metabolismo
Glucagon/metabolismo
Hipoglicemiantes/farmacologia
Insulina/metabolismo
Peptídeos/farmacologia
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Cricetinae
Polipeptídeo Inibidor Gástrico/metabolismo
Peptídeo 1 Semelhante ao Glucagon/metabolismo
Glucose/metabolismo
Células HEK293
Seres Humanos
Células Secretoras de Insulina/efeitos dos fármacos
Células Secretoras de Insulina/metabolismo
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Knockout
Receptores dos Hormônios Gastrointestinais/metabolismo
Receptores de Glucagon/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hypoglycemic Agents); 0 (Insulin); 0 (Peptides); 0 (Receptors, Gastrointestinal Hormone); 0 (Receptors, Glucagon); 0 (gastric inhibitory polypeptide receptor); 59392-49-3 (Gastric Inhibitory Polypeptide); 89750-14-1 (Glucagon-Like Peptide 1); 9007-92-5 (Glucagon); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160516
[St] Status:MEDLINE


  8 / 1230 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26989170
[Au] Autor:Steventon B; Duarte F; Lagadec R; Mazan S; Nicolas JF; Hirsinger E
[Ad] Endereço:Department of Developmental and Stem Cell Biology, Institut Pasteur, 25 rue du Docteur Roux, Paris cedex 15 75724, France bjs57@cam.ac.uk.
[Ti] Título:Species-specific contribution of volumetric growth and tissue convergence to posterior body elongation in vertebrates.
[So] Source:Development;143(10):1732-41, 2016 05 15.
[Is] ISSN:1477-9129
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Posterior body elongation is a widespread mechanism propelling the generation of the metazoan body plan. The posterior growth model predicts that a posterior growth zone generates sufficient tissue volume to elongate the posterior body. However, there are energy supply-related differences between vertebrates in the degree to which growth occurs concomitantly with embryogenesis. By applying a multi-scalar morphometric analysis in zebrafish embryos, we show that posterior body elongation is generated by an influx of cells from lateral regions, by convergence-extension of cells as they exit the tailbud, and finally by a late volumetric growth in the spinal cord and notochord. Importantly, the unsegmented region does not generate additional tissue volume. Fibroblast growth factor inhibition blocks tissue convergence rather than volumetric growth, showing that a conserved molecular mechanism can control convergent morphogenesis through different cell behaviours. Finally, via a comparative morphometric analysis in lamprey, dogfish, zebrafish and mouse, we propose that elongation via posterior volumetric growth is linked to increased energy supply and is associated with an overall increase in volumetric growth and elongation.
[Mh] Termos MeSH primário: Padronização Corporal
Organogênese
Vertebrados/embriologia
[Mh] Termos MeSH secundário: Animais
Movimento Celular
Proliferação Celular
Cação (Peixe)/embriologia
Fatores de Crescimento de Fibroblastos/metabolismo
Lampreias/embriologia
Camundongos
Notocorda/embriologia
Transdução de Sinais
Especificidade da Espécie
Medula Espinal/embriologia
Cauda
Peixe-Zebra/embriologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
62031-54-3 (Fibroblast Growth Factors)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171124
[Lr] Data última revisão:
171124
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160319
[St] Status:MEDLINE
[do] DOI:10.1242/dev.126375


  9 / 1230 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26631425
[Au] Autor:Huang K; Xu K; Zhu W; Yang L; Hou X; Zheng C
[Ad] Endereço:Key Laboratory of Green Chemistry and Technology of MOE, College of Chemistry, Sichuan University , Chengdu, Sichuan 610064, China.
[Ti] Título:Hydride Generation for Headspace Solid-Phase Extraction with CdTe Quantum Dots Immobilized on Paper for Sensitive Visual Detection of Selenium.
[So] Source:Anal Chem;88(1):789-95, 2016 Jan 05.
[Is] ISSN:1520-6882
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A low-cost, simple, and highly selective analytical method was developed for sensitive visual detection of selenium in human urine both outdoors and at home, by coupling hydride generation with headspace solid-phase extraction using quantum dots (QDs) immobilized on paper. The visible fluorescence from the CdTe QDs immobilized on paper was quenched by H2Se from hydride generation reaction and headspace solid-phase extraction. The potential mechanism was investigated by using X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS) as well as Density Functional Theory (DFT). Potential interferences from coexisting ions, particularly Ag(+), Cu(2+), and Zn(2+), were eliminated. The selectivity was significantly increased because the selenium hydride was effectively separated from sample matrices by hydride generation. Moreover, due to the high sampling efficiency of hydride generation and headspace solid phase extraction, the sensitivity and the limit of detection (LOD) were significantly improved compared to conventional methods. A LOD of 0.1 µg L(-1) and a relative standard deviation (RSD, n = 7) of 2.4% at a concentration of 20 µg L(-1) were obtained when using a commercial spectrofluorometer as the detector. Furthermore, a visual assay based on the proposed method was developed for the detection of Se, 5 µg L(-1) of selenium in urine can be discriminated from the blank solution with the naked eye. The proposed method was validated by analysis of certified reference materials and human urine samples with satisfactory results.
[Mh] Termos MeSH primário: Compostos de Cádmio/química
Papel
Pontos Quânticos
Selênio/urina
Extração em Fase Sólida
Telúrio/química
[Mh] Termos MeSH secundário: Animais
Compostos de Cádmio/síntese química
Cação (Peixe)
Cabelo/química
Seres Humanos
Músculos/química
Teoria Quântica
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Cadmium Compounds); H6241UJ22B (Selenium); NQA0O090ZJ (Tellurium); STG188WO13 (cadmium telluride)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:161126
[Lr] Data última revisão:
161126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151204
[St] Status:MEDLINE
[do] DOI:10.1021/acs.analchem.5b03128


  10 / 1230 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26471575
[Au] Autor:Valdersnes S; Fecher P; Maage A; Julshamn K
[Ad] Endereço:National Institute of Nutrition and Seafood Research (NIFES), P.O. Box 2029, Nordnes, N-5817 Bergen, Norway. Electronic address: stig.valdersnes@nifes.no.
[Ti] Título:Collaborative study on determination of mono methylmercury in seafood.
[So] Source:Food Chem;194:424-31, 2016 Mar 01.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Eight laboratories participated in an inter-laboratory method-performance (collaborative) study of a method for the determination of mono methylmercury (MMHg) in foodstuffs of marine origin by gas chromatography inductively coupled plasma isotope dilution mass spectrometry (GC-ICP-IDMS) after dissolution, derivatisation and extraction of the species. The method was tested on seven seafood products covering both a wide concentration range and variations in the MMHg concentrations as well as matrix compositions. The samples were mussel tissue, squid muscle, crab claw meat, whale meat, cod muscle, Greenland halibut muscle and dogfish liver (NRCC DOLT-4), with MMHg concentrations ranging from 0.035 to 3.58mg/kg (as Hg) dry weight. Repeatability relative standard deviations (RSDr) for MMHg ranged from 2.1% to 8.7%. Reproducibility relative standard deviations (RSDR) ranged from 5.8% to 42%. All samples showed HorRat value below 1.0, except for the sample with the lowest MMHg content, mussel tissue, with a HorRat value of 1.6.
[Mh] Termos MeSH primário: Análise de Alimentos/métodos
Contaminação de Alimentos/análise
Mercúrio/análise
Compostos de Metilmercúrio/análise
Alimentos Marinhos/análise
[Mh] Termos MeSH secundário: Animais
Bivalves/química
Cromatografia Gasosa
Cação (Peixe)
Linguado
Concentração de Íons de Hidrogênio
Técnicas de Diluição do Indicador
Isótopos
Fígado/química
Espectrometria de Massas
Músculos/química
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Isotopes); 0 (Methylmercury Compounds); FXS1BY2PGL (Mercury)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:151016
[Lr] Data última revisão:
151016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151017
[St] Status:MEDLINE



página 1 de 123 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde