Base de dados : MEDLINE
Pesquisa : B01.050.150.900.493.418 [Categoria DeCS]
Referências encontradas : 1702 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 171 ir para página                         

  1 / 1702 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29203198
[Au] Autor:Roman-Padilla J; Rodríguez-Rúa A; Carballo C; Manchado M; Hachero-Cruzado I
[Ad] Endereço:IFAPA Centro El Toruño, Junta de Andalucía, Camino Tiro Pichón s/n, El Puerto de Santa María, Cádiz 11500, Spain.
[Ti] Título:Phylogeny and expression patterns of two apolipoprotein E genes in the flatfish Senegalese sole.
[So] Source:Gene;643:7-16, 2018 Feb 15.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The apolipoprotein E (ApoE) is a key component of several lipoproteins involved in lipid homeostasis. In this study, two cDNA sequences encoding ApoE (referred to as apoEa and apoEb) were characterized in the flatfish Solea senegalensis. The predicted peptides contained conserved structural blocks related with their capacity for lipid binding and lipoprotein receptor interaction. At genomic level, both genes contained five exons and four introns and they were organized into two tandem arrays with apoA-IV gene copies. The phylogenetic analysis clearly separated them into two well-supported clusters that matched with their organization in the genome of teleosts. Whole-mount in situ hybridization located the apoEa signal in the yolk syncytial layer (YSL) of lecitothrophic larval stages (0dph) and in the anterior intestine of exotrophic larvae and benthic fish. In the case of apoEb, hybridization signals were located in the YSL, tail bud, eyes and mouth at 0dph and in the otic vesicle, hindbrain, eyes, pharynx, mouth, heart and intestine at 1dph. In exotrophic larvae, apoEb was ubiquitously expressed in several tissues such as taste buds, brain, mouth, nostril, gills, intestine, liver and around the neuromasts and eyes. Quantification of mRNA levels in pools of whole larvae confirmed distinct expression patterns with a significant reduction of apoEa and an increase of apoEb mRNA levels throughout larval development. Moreover, only apoEa transcripts increased in response to food supply suggesting that this paralog mostly participates in the absorption and transport of dietary lipids and the apoEb in the redistribution of endogenous lipids as well as in neural tissue regeneration.
[Mh] Termos MeSH primário: Apolipoproteínas E/genética
Linguados/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos/genética
Animais
Apolipoproteínas E/metabolismo
Sequência Conservada/genética
Linguados/embriologia
Perfilação da Expressão Gênica/métodos
Regulação da Expressão Gênica no Desenvolvimento/genética
Genoma
Genômica
Hibridização In Situ
Larva/genética
Filogenia
RNA Mensageiro/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Apolipoproteins E); 0 (RNA, Messenger)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180123
[Lr] Data última revisão:
180123
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171206
[St] Status:MEDLINE


  2 / 1702 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29246535
[Au] Autor:Taboada X; Rey M; Bouza C; Viñas A
[Ad] Endereço:Departamento de Zoología, Genética y Antropología Física, Facultad de Biología, CIBUS, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain.
[Ti] Título:Cytogenomic analysis of several repetitive DNA elements in turbot (Scophthalmus maximus).
[So] Source:Gene;644:4-12, 2018 Feb 20.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Repetitive DNA plays a fundamental role in the organization, size and evolution of eukaryotic genomes. The sequencing of the turbot revealed a small and compact genome, as in all flatfish studied to date. The assembly of repetitive regions is still incomplete because it is difficult to correctly identify their position, number and array. The combination of classical cytogenetic techniques along with high quality sequencing is essential to increase the knowledge of the structure and composition of these sequences and, thus, of the structure and function of the whole genome. In this work, the in silico analysis of H1 histone, 5S rDNA, telomeric and Rex repetitive sequences, was compared to their chromosomal mapping by fluorescent in situ hybridization (FISH), providing a more comprehensive picture of these elements in the turbot genome. FISH assays confirmed the location of H1 in LG8; 5S rDNA in LG4 and LG6; telomeric sequences at the end of all chromosomes whereas Rex elements were dispersed along most chromosomes. The discrepancies found between both approaches could be related to the sequencing methodology applied in this species and also to the resolution limitations of the FISH technique. Turbot cytogenomic analyses have proven to add new chromosomal landmarks in the karyotype of this species, representing a powerful tool to investigate targeted genomic sequences or regions in the genetic and physical maps of this species.
[Mh] Termos MeSH primário: DNA/genética
Linguados/genética
Sequências Repetitivas de Ácido Nucleico/genética
[Mh] Termos MeSH secundário: Animais
Mapeamento Cromossômico/métodos
Análise Citogenética/métodos
Genoma/genética
Hibridização in Situ Fluorescente/métodos
Cariótipo
RNA Ribossômico 5S/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 5S); 9007-49-2 (DNA)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180119
[Lr] Data última revisão:
180119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171217
[St] Status:MEDLINE


  3 / 1702 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29053706
[Au] Autor:Riesco MF; Oliveira C; Soares F; Gavaia PJ; Dinis MT; Cabrita E
[Ad] Endereço:CCMAR, University of Algarve, Campus of Gambelas, Faro, Portugal.
[Ti] Título:Solea senegalensis sperm cryopreservation: New insights on sperm quality.
[So] Source:PLoS One;12(10):e0186542, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cryopreservation of Senegalese sole sperm can represent an alternative to overcome some reproductive problems of this species. However, it is important to guarantee the safe use of cryopreserved sperm by selecting an appropriate protocol according to a high demand quality need to be ensured. It has been demonstrated that traditional assays such as motility and viability do not provide enough information to identify specific damage caused by cryopreservation process (freezing and thawing). Specific tests, including lipid peroxidation and DNA damage, should be performed. In the present study, motility and lipid peroxidation were performed as specific tests allowing us to discard cryopreservation conditions such as methanol as internal cryoprotectant and bovine serum albumin as external cryoprotectant. In addition, a caspase 3/7 detection by flow cytometry was performed to analyze apoptosis activity in the best selected conditions. Moreover, new highly sensitive tests based on transcript number detection have recently been described in fish sperm cryopreservation. For this reason, a transcript level detection assay was performed on certain oxidative and chaperone genes related to fertilization ability and embryo development (hsp70, hsp90BB, hsp90AA, gpx) to select the best cryopreservation conditions. DMSO+ egg yolk proved to be the best cryoprotectant combination in terms of transcript level. This study describes an optimized cryopreservation protocol for Solea senegalensis sperm demonstrating for the first time that transcript degradation is the most sensitive predictor of cell status in this species after cryopreservation.
[Mh] Termos MeSH primário: Criopreservação/métodos
Linguados
Preservação do Sêmen/métodos
[Mh] Termos MeSH secundário: Animais
Masculino
Espermatozoides/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171021
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186542


  4 / 1702 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28946122
[Au] Autor:Cho SH; Park JA; Zheng W; Abd El-Aty AM; Kim SK; Choi JM; Yi H; Cho SM; Afifi NA; Shim JH; Chang BJ; Kim JS; Shin HC
[Ad] Endereço:Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea.
[Ti] Título:Quantification of bupivacaine hydrochloride and isoflupredone acetate residues in porcine muscle, beef, milk, egg, shrimp, flatfish, and eel using a simplified extraction method coupled with liquid chromatography-triple quadrupole tandem mass spectrometry.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1065-1066:29-34, 2017 Oct 15.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In this study, a simple analytical approach has been developed and validated for the determination of bupivacaine hydrochloride and isoflupredone acetate residues in porcine muscle, beef, milk, egg, shrimp, flatfish, and eel using liquid chromatography-tandem mass spectrometry (LC-MS/MS). A 0.1% solution of acetic acid in acetonitrile combined with n-hexane was used for deproteinization and defatting of all tested matrices and the target drugs were well separated on a Waters Xbridge™ C18 analytical column using a mobile phase consisting of 0.1% acetic acid (A) and 0.1% solution of acetic acid in methanol (B). The linearity estimated from six-point matrix-matched calibrations was good, with coefficients of determination ≥0.9873. The limits of quantification (LOQs) for bupivacaine hydrochloride and isoflupredone acetate were 1 and 2ngg , respectively. Recovery percentages in the ranges of 72.51-112.39% (bupivacaine hydrochloride) and 72.58-114.56% (isoflupredone acetate) were obtained from three different fortification concentrations with relative standard deviations (RSDs) of <15.14%. All samples for the experimental work and method application were collected from the local markets in Seoul, Republic of Korea, and none of them tested positive for the target drugs. In conclusion, a simple method using a 0.1% solution of acetic acid in acetonitrile and n-hexane followed by LC-MS/MS could effectively extract bupivacaine hydrochloride and isoflupredone acetate from porcine muscle, beef, milk, egg, shrimp, flatfish, and eel samples.
[Mh] Termos MeSH primário: Bupivacaína/análise
Resíduos de Drogas/análise
Fluprednisolona/análogos & derivados
Leite/química
Carne Vermelha/análise
Alimentos Marinhos/análise
[Mh] Termos MeSH secundário: Animais
Cromatografia Líquida/métodos
Enguias
Linguados
Fluprednisolona/análise
Modelos Lineares
Penaeidae
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
Suínos
Espectrometria de Massas em Tandem/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
55P9TUL75S (isoflupredone acetate); 9H05937G3X (Fluprednisolone); Y8335394RO (Bupivacaine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170926
[St] Status:MEDLINE


  5 / 1702 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28877259
[Au] Autor:Fatsini E; Rey S; Ibarra-Zatarain Z; Mackenzie S; Duncan NJ
[Ad] Endereço:IRTA, Sant Carles de la Ràpita, Tarragona, Spain.
[Ti] Título:Dominance behaviour in a non-aggressive flatfish, Senegalese sole (Solea senegalensis) and brain mRNA abundance of selected transcripts.
[So] Source:PLoS One;12(9):e0184283, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Dominance is defined as the preferential access to limited resources. The present study aimed to characterise dominance in a non-aggressive flatfish species, the Senegalese sole (Solea senegalensis) by 1) identifying dominance categories and associated behaviours and 2) linking dominance categories (dominant and subordinate) with the abundance of selected mRNA transcripts in the brain. Early juveniles (n = 74, 37 pairs) were subjected to a dyadic dominance test, related to feeding, and once behavioural phenotypes had been described the abundance of ten selected mRNAs related to dominance and aggressiveness was measured in the brain. Late juveniles were subjected to two dyadic dominance tests (n = 34, 17 pairs), related to feeding and territoriality and one group test (n = 24, 4 groups of 6 fish). Sole feeding first were categorized as dominant and sole feeding second or not feeding as subordinate. Three social behaviours (i. "Resting the head" on another fish, ii. "Approaching" another fish, iii. "Swimming above another" fish) were associated with dominance of feeding. Two other variables (i. Total time occupying the preferred area during the last 2 hours of the 24 h test, ii. Organisms occupying the preferred area when the test ended) were representative of dominance in the place preference test. In all tests, dominant fish compared to subordinate fish displayed a significantly higher number of the behaviours "Rest the head" and "Approaches". Moreover, dominant sole dominated the sand at the end of the test, and in the group test dominated the area close to the feed delivery point before feed was delivered. The mRNA abundance of the selected mRNAs related to neurogenesis (nrd2) and neuroplasticity (c-fos) in dominant sole compared to subordinate were significantly different. This is the first study to characterise dominance categories with associated behaviours and mRNA abundance in Senegalese sole and provides tools to study dominance related problems in feeding and reproduction in aquaculture.
[Mh] Termos MeSH primário: Comportamento Animal
Encéfalo/metabolismo
Linguados/fisiologia
Regulação da Expressão Gênica
Predomínio Social
[Mh] Termos MeSH secundário: Agressão
Animais
Comportamento Alimentar
Perfilação da Expressão Gênica
Neurogênese
Plasticidade Neuronal
Análise de Componente Principal
RNA Mensageiro/metabolismo
Natação
Territorialidade
Gravação em Vídeo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Messenger)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184283


  6 / 1702 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28837403
[Au] Autor:Holth TF; Storset A; Ribeiro AL; Ólafsdóttir Á; Halldórsson HP; Hylland K
[Ad] Endereço:a Department of Biosciences , University of Oslo , Blindern , Oslo , Norway.
[Ti] Título:Environmentally realistic exposure to weathered North Sea oil: Sublethal effects in Atlantic cod (Gadus morhua) and turbot (Scophthalmus maximus).
[So] Source:J Toxicol Environ Health A;80(16-18):895-906, 2017.
[Is] ISSN:1528-7394
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:With increasing oil and gas activities and transport in the Arctic, there is a need to understand how operational or accidental releases of substances affect marine organisms from a pristine environment. The aim of the current study was to describe and compare the responses of two marine fish species, Atlantic cod (Gadus morhua) and turbot (Scophthalmus maximus), following exposure to three levels (low, medium, high) of the water-soluble fraction of a North Sea crude oil for 16 days. The exposure system simulated environmental exposure by allowing clean seawater to percolate through gravel covered in weathered oil before being introduced to aquaria. Both polycyclic aromatic hydrocarbon (PAH) metabolite bile concentrations and cytochrome P4501A (CYP1A) levels and activity increased markedly in comparison with controls in both species, but there were no significant differences between the three exposures. Turbot possessed 4-5-fold higher concentrations of two PAH bile metabolites compared to Atlantic cod by day 8. In contrast, hepatic CYP1A activity in cod was consistently 2-6-fold higher than in turbot with increasing differences over the experimental period. Baseline DNA strand breaks in lymphocytes and kidney cells were low in both species, but was elevated for all treatments by day two. There were no marked indications of the treatments affecting immune functions in either species. This investigation demonstrated that there may be significant differences in responses between species receiving identical exposures and that DNA strand breaks in lymphocytes and kidney cells are sensitive to confinement stress. Data also indicate that some species, such as turbot, may adapt to treatments within days and weeks.
[Mh] Termos MeSH primário: Exposição Ambiental/efeitos adversos
Linguados/metabolismo
Gadus morhua/metabolismo
Petróleo/toxicidade
Água do Mar/química
Poluentes Químicos da Água/toxicidade
[Mh] Termos MeSH secundário: Animais
Hidrocarboneto de Aril Hidroxilases/metabolismo
Bile/química
Contaminação de Alimentos/análise
Leucócitos Mononucleares/efeitos dos fármacos
Leucócitos Mononucleares/metabolismo
Fígado/efeitos dos fármacos
Fígado/metabolismo
Mar do Norte
Hidrocarbonetos Aromáticos Policíclicos/toxicidade
Explosão Respiratória/efeitos dos fármacos
Alimentos Marinhos/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Petroleum); 0 (Polycyclic Aromatic Hydrocarbons); 0 (Water Pollutants, Chemical); EC 1.14.14.1 (Aryl Hydrocarbon Hydroxylases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170825
[St] Status:MEDLINE
[do] DOI:10.1080/15287394.2017.1352195


  7 / 1702 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28831954
[Au] Autor:Xu H; Cao L; Wei Y; Zhang Y; Liang M
[Ad] Endereço:1Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,106 Nanjing Road,Qingdao 266071,People's Republic of China.
[Ti] Título:Effects of different dietary DHA:EPA ratios on gonadal steroidogenesis in the marine teleost, tongue sole (Cynoglossus semilaevis).
[So] Source:Br J Nutr;118(3):179-188, 2017 Aug.
[Is] ISSN:1475-2662
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The present study was conducted to investigate the effects of dietary DHA and EPA on gonadal steroidogenesis in mature females and males, with a feeding trial on tongue sole, a typical marine teleost with sexual dimorphism. Three experimental diets differing basically in DHA:EPA ratio, that is, 0·68 (diet D:E-0·68), 1·09 (D:E-1·09) and 2·05 (D:E-2·05), were randomly assigned to nine tanks of 3-year-old tongue sole (ten females and fifteen males in each tank). The feeding trail lasted for 90 d before and during the spawning season. Fish were reared in a flowing seawater system and fed to apparent satiation twice daily. Compared with diet D:E-0·68, diet D:E-1·09 significantly enhanced the oestradiol production in females, whereas diet D:E-2·05 significantly enhanced the testosterone production in males. In ovaries, diet D:E-1·09 induced highest mRNA expression of follicle-stimulating hormone receptor (FSHR), steroidogenic acute regulatory protein, 17α-hydroxylase (P450c17) and 3ß-hydroxysteroid dehydrogenase (3ß-HSD). In testes, diet 2·05 resulted in highest mRNA expression of FSHR, cholesterol side-chain cleavage enzyme, P450c17 and 3ß-HSD. Fatty acid profiles in fish tissues reflected closely those of diets. Female fish had more gonadal EPA content but less DHA content than male fish, whereas there was a reverse observation in liver. In conclusion, the dietary DHA:EPA ratio, possibly combined with the dietary EPA:arachidonic acid ratio, differentially regulated sex steroid hormone synthesis in mature female and male tongue soles. Females seemed to require more EPA but less DHA for the gonadal steroidogenesis than males. The results are beneficial to sex-specific nutritive strategies in domestic teleost.
[Mh] Termos MeSH primário: Dieta/veterinária
Ácidos Docosa-Hexaenoicos/administração & dosagem
Ácido Eicosapentaenoico/administração & dosagem
Linguados/metabolismo
Hormônios Esteroides Gonadais/biossíntese
Gônadas/efeitos dos fármacos
[Mh] Termos MeSH secundário: 17-Hidroxiesteroide Desidrogenases/genética
17-Hidroxiesteroide Desidrogenases/metabolismo
Animais
Ácido Araquidônico/administração & dosagem
Ácido Araquidônico/análise
Ácidos Docosa-Hexaenoicos/análise
Ácido Eicosapentaenoico/análise
Estradiol/biossíntese
Estradiol/sangue
Feminino
Hormônios Esteroides Gonadais/sangue
Gônadas/metabolismo
Lipogênese/efeitos dos fármacos
Masculino
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Receptores do FSH/genética
Receptores do FSH/metabolismo
Esteroide 17-alfa-Hidroxilase/genética
Esteroide 17-alfa-Hidroxilase/metabolismo
Testosterona/biossíntese
Testosterona/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Gonadal Steroid Hormones); 0 (RNA, Messenger); 0 (Receptors, FSH); 25167-62-8 (Docosahexaenoic Acids); 27YG812J1I (Arachidonic Acid); 3XMK78S47O (Testosterone); 4TI98Z838E (Estradiol); AAN7QOV9EA (Eicosapentaenoic Acid); EC 1.1.- (17-Hydroxysteroid Dehydrogenases); EC 1.1.1.51 (3 (or 17)-beta-hydroxysteroid dehydrogenase); EC 1.14.14.19 (Steroid 17-alpha-Hydroxylase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170907
[Lr] Data última revisão:
170907
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170824
[St] Status:MEDLINE
[do] DOI:10.1017/S0007114517001891


  8 / 1702 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28570893
[Au] Autor:Pouil S; Oberhänsli F; Bustamante P; Metian M
[Ad] Endereço:International Atomic Energy Agency - Environment Laboratories (IAEA-EL), 4 Quai Antoine 1er, MC-98000, Monaco; Littoral Environnement et Sociétés (LIENSs), UMR 7266 CNRS - Université de La Rochelle, 2 rue Olympe de Gouges, F-17000 La Rochelle, France.
[Ti] Título:Dietary Zn and the subsequent organotropism in fish: No influence of food quality, frequency of feeding and environmental conditions (pH and temperature).
[So] Source:Chemosphere;183:503-509, 2017 Sep.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Trophic transfer of Zn in fish is affected by the type of food and environmental variables such as temperature. However, there is still a lack of knowledge regarding the effects of such factors on Zn organotropism. For this reason, a series of experimental studies have investigated how the distribution and the concentration of Zn is affected by some environmentally-relevant factors (food quality, food availability, water pH, and temperature) in turbot Scophthalmus maximus using radiotracer techniques. In three different experiments, Zn distribution in seven body compartments of juvenile turbot and the calculation of Zn concentration index (I ) for each compartment were compared. Its distribution as well as its concentration in the body compartments of juvenile turbots were not affected by the experimental conditions tested. This apparent consistency in the Zn organotropism can be explained by the ability of the fish to maintain Zn homeostasis at non-toxic Zn concentrations in their diet. These results are important to better understand the trophic transfer of Zn in fish under realistic environmental conditions.
[Mh] Termos MeSH primário: Dieta
Linguados/metabolismo
Poluentes Químicos da Água/metabolismo
Zinco/metabolismo
[Mh] Termos MeSH secundário: Ração Animal
Animais
Comportamento Alimentar
Cadeia Alimentar
Qualidade dos Alimentos
Concentração de Íons de Hidrogênio
Especificidade de Órgãos
Alimentos Marinhos
Temperatura Ambiente
Distribuição Tecidual
Poluentes Químicos da Água/farmacocinética
Poluentes Químicos da Água/toxicidade
Zinco/farmacocinética
Zinco/toxicidade
Radioisótopos de Zinco
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Water Pollutants, Chemical); 0 (Zinc Radioisotopes); J41CSQ7QDS (Zinc)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170602
[St] Status:MEDLINE


  9 / 1702 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28492177
[Au] Autor:López JR; Lorenzo L; Alcantara R; Navas JI
[Ad] Endereço:IFAPA Centro Agua del Pino, Junta de Andalucía, Carretera El Rompido-Punta Umbría km 3.8, CP21450 Cartaya, Huelva, Spain.
[Ti] Título:Characterization of Aliivibrio fischeri strains associated with disease outbreak in brill Scophthalmus rhombus.
[So] Source:Dis Aquat Organ;124(3):215-222, 2017 05 11.
[Is] ISSN:0177-5103
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Three bacterial isolates were recovered from a disease outbreak with high mortality affecting brill Scophthalmus rhombus (Linnaeus, 1758). Moribund fish showed no external signs of disease, but plentiful haemorrhages were observed in liver. On the basis of phenotypic and genotypic characterization, the isolates were identified as Aliivibrio fischeri. The phenotypic profile of the isolates was basically similar to that of the type strain of this species, although some discrepancies were observed, mainly in the BIOLOG GN profile. The main cellular fatty acids of strain a591 were also consistent with this species. The highest 16S rDNA sequence similarities were recorded with the type strain of A. fischeri (99.07%); other Aliivibrio species showed similarity values below 96%. The highest sequence similarities with gyrB, rpoD and recA genes were also recorded with A. fischeri type strain (99.31, 98.99 and 95.29% similarity, respectively). DNA-DNA hybridization assays confirmed that these isolates belong to A. fischeri; levels of DNA relatedness were 73.5 to 86.2% with isolate a591 (reciprocal values of 86.9 to 99.04%). Finally, a virulence evaluation of the isolates using Senegalese sole fry was also performed; significant mortalities (100% mortality within 5 d) were recorded by intraperitoneal injection, but only with high doses of bacteria (2 × 106 cfu g-1 body weight).
[Mh] Termos MeSH primário: Aliivibrio fischeri/genética
Surtos de Doenças/veterinária
Doenças dos Peixes/microbiologia
Linguados/microbiologia
Infecções por Bactérias Gram-Negativas/veterinária
[Mh] Termos MeSH secundário: Animais
Aquicultura
DNA Bacteriano/genética
Infecções por Bactérias Gram-Negativas/microbiologia
Filogenia
Reação em Cadeia da Polimerase
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Bacterial)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170803
[Lr] Data última revisão:
170803
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170512
[St] Status:MEDLINE
[do] DOI:10.3354/dao03123


  10 / 1702 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28489928
[Au] Autor:Zhu Y; Hu Q; Xu W; Li H; Guo H; Meng L; Wei M; Lu S; Shao C; Wang N; Yang G; Chen S
[Ad] Endereço:College of Marine Life Science, Ocean University of China, Qingdao, China.
[Ti] Título:Identification and analysis of the ß-catenin1 gene in half-smooth tongue sole (Cynoglossus semilaevis).
[So] Source:PLoS One;12(5):e0176122, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:ß-catenin is a key signalling molecule in the canonical Wnt pathway, which plays a role in cell adhesion, embryogenesis and sex determination. However, little is known about its function in teleosts. We cloned and characterized the full-length ß-catenin1 gene from half-smooth tongue sole (Cynoglossus semilaevis), which was designated CS-ß-catenin1. The CS-ß-catenin1 cDNA consists of 2,346 nucleotides and encodes a protein with 782 amino acids. Although CS-ß-catenin1 was transcribed in the gonads of both sexes, the level was significantly higher in ovaries compared to testes. Furthermore, the mRNA level of CS-ß-catenin1 was significantly upregulated at 160 days and constantly increased until 2 years of age. In situ hybridization revealed that CS-ß-catenin1 mRNA was mainly localized in oocyte cells, especially in stage I, II and III oocytes. When CS-ß-catenin1 expression was inhibited by injection of quercetin in the ovaries, levels of CS-Figla and CS-foxl2 mRNA were significantly down-regulated, and CS-dmrt1 was up-regulated, which suggested that CS-ß-catenin1 is a potential upstream gene of CS-Figla and is involved in the development of the ovaries, i.e., folliculogenesis.
[Mh] Termos MeSH primário: Linguados/genética
beta Catenina/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Sequência de Bases
Metilação de DNA
Feminino
Masculino
Ovário/metabolismo
Filogenia
Testículo/metabolismo
beta Catenina/química
beta Catenina/classificação
beta Catenina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (beta Catenin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170511
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0176122



página 1 de 171 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde