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[PMID]:29256424
[Au] Autor:Bigarré L; Plassiart G; de Boisséson C; Pallandre L; Pozet F; Ledoré Y; Fontaine P; Lieffrig F
[Ad] Endereço:ANSES, Laboratoire Ploufragan-Plouzané, 29280 Plouzané, France.
[Ti] Título:Molecular investigations of outbreaks of Perch perhabdovirus infections in pike-perch.
[So] Source:Dis Aquat Organ;127(1):19-27, 2017 Dec 19.
[Is] ISSN:0177-5103
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:In 2016, a total of 5 massive mortality episodes each affecting hundreds of thousands of pike-perch Sander lucioperca larvae occurred at 2 sites in 2 Western European countries. For each episode, perhabdoviruses related to the perch rhabdovirus (PRV) were detected in samples, using either PCR or cell culture combined with PCR. The sequences of the glycoprotein (g), phosphoprotein (p) and nucleoprotein (n) genes of these samples demonstrated that 2 different genotypes were present at 1 site, each associated with 1 of the 3 episodes. At the other site, a single genotype was associated with the 2 outbreaks. Furthermore, this genotype was strictly identical to 1 genotype involved in the outbreaks of the first site, strongly suggesting a common origin for these 2 viruses. The common origin was confirmed a posteriori because some larvae introduced to both sites had exactly the same geographic origin in Eastern Europe. Taken together, the molecular and epidemiological data suggest that both horizontal and vertical transmission of 2 distinct strains of perhabdoviruses were involved in the various outbreaks affecting pike-perch.
[Mh] Termos MeSH primário: Surtos de Doenças/veterinária
Doenças dos Peixes/virologia
Perciformes/virologia
Infecções por Rhabdoviridae/veterinária
Rhabdoviridae
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Europa (Continente)/epidemiologia
Doenças dos Peixes/epidemiologia
Larva/virologia
Rhabdoviridae/genética
Infecções por Rhabdoviridae/epidemiologia
Infecções por Rhabdoviridae/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171220
[St] Status:MEDLINE
[do] DOI:10.3354/dao03177


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[PMID]:29278825
[Au] Autor:Di Azevedo MIN; Iñiguez AM
[Ad] Endereço:LABTRIP, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro 21045-900, Brazil.
[Ti] Título:Nematode parasites of commercially important fish from the southeast coast of Brazil: Morphological and genetic insight.
[So] Source:Int J Food Microbiol;267:29-41, 2018 02 21.
[Is] ISSN:1879-3460
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Studies of nematofauna of teleost fish from the Brazilian coast are relatively scarce and limited to identification based on morphology. The objective of the present study was to determine the diversity and prevalence of nematode parasites in teleost fish from the southeast Atlantic coast of Rio de Janeiro, through morphological, molecular, and ecological approaches. Parasites were collected from sixty specimens each of Genypterus brasiliensis, Micropogonias furnieri, and Mullus argentinae obtained in winters and summers of 2012­2014. Morphological and genetic characterization was conducted using light microscopy and the molecular targets 18S rDNA, ITS1, and mtDNA cox2. Nematodes identified in M. furnieri were Cucculanus genypteri (n = 1575, P = 98.3%) and Hysterothylacium deardorffoverstreetorum (s.l.) (n = 2, P = 3.3%); in G. brasiliensis were Dichelyne (Cucullanellus) sciaenidicola (n = 99, P = 33.3%), Cucculanus pulcherrimus (n = 45, P = 18.3%), Hysterothylacium deardorffoverstreetorum (s.l.) (n = 3, P = 5%), and Anisakis typica (n = 1, P = 1.7%); and, in M. argentinae, were H. deardorffoverstreetorum (s.l.) (n = 146, P = 48.3%), and Procamallanus (Spirocamallanus) halitrophus (n = 4, P = 6.7%). DNA sequence data of C. genypteri, C. pulcherrimus, D. (C.) sciaenidicola, and P. (S.) halitrophus were reported for the first time. New host records are M. argentinae for P. (S.) halitrophus, M. furnieri for A. typica, while H. deardorffoverstreetorum (s.l.) was found in all three fish species. Intestine showed significantly higher intensity than other sites, and no significant seasonal variation in parasitological indices was observed. Hysterothylacium specimens (n = 6) were found in fish muscle, potentially a public health concern.
[Mh] Termos MeSH primário: Doenças dos Peixes/parasitologia
Nematoides/genética
Infecções por Nematoides/parasitologia
Perciformes/parasitologia
[Mh] Termos MeSH secundário: Animais
Brasil
DNA Mitocondrial/genética
DNA Ribossômico/genética
Nematoides/classificação
Nematoides/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Mitochondrial); 0 (DNA, Ribosomal)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171227
[St] Status:MEDLINE


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[PMID]:29037697
[Au] Autor:Hu Y; Shao Y; Wu C; Yuan C; Ishimura G; Liu W; Chen S
[Ad] Endereço:National Engineering Laboratory of Intelligent Food Technology and Equipment, Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture, Key Laboratory for Agro-Products Nutritional Evaluation of Ministry of Agriculture, Zhejiang Key Laboratory for Agro-Food Processing, Fuli I
[Ti] Título:γ-PGA and MTGase improve the formation of ε-(γ-glutamyl) lysine cross-links within hairtail (Trichiurus haumela) surimi protein.
[So] Source:Food Chem;242:330-337, 2018 Mar 01.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The present study investigated the mechanism of ε-(γ-glutamyl) lysine cross-links within hairtail (Trichiurus haumela) surimi protein via γ-polyglutamic acid (γ-PGA) and MTGase. The results indicated that the addition of MTGase and γ-PGA markedly improved the gelation properties of hairtail surimi protein, including its maximum breaking force and deformation, water holding capacity and gel strength. The maximum improvements were achieved by adding 0.5units MTGase/g meat paste in combination with 0.06% γ-PGA. SDS-PAGE showed that the band intensity of cross-linked proteins increased, whereas that of myosin heavy chain decreased after treatments. Further scanning electron microscopy (SEM) analysis showed the formation of a denser gel matrix, which was caused by much stronger and more inter- and intra-molecular cross-linking of proteins, via MTGase catalysing ε-(γ-glutamyl) lysine cross-links formed between lysine residues in the gel protein and glutamic residues in the hydrolytic γ-PGA. The results provide reliable guidance for the improvement of hairtail surimi protein gelation properties.
[Mh] Termos MeSH primário: Produtos Pesqueiros
Manipulação de Alimentos/métodos
Géis/química
Lisina/química
Perciformes
Ácido Poliglutâmico/análogos & derivados
[Mh] Termos MeSH secundário: Animais
Eletroforese em Gel de Poliacrilamida
Lisina/metabolismo
Ácido Poliglutâmico/química
Ácido Poliglutâmico/metabolismo
Transglutaminases/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Gels); 0 (poly(gamma-glutamic acid)); 25513-46-6 (Polyglutamic Acid); EC 2.3.2.13 (Transglutaminases); K3Z4F929H6 (Lysine)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171018
[St] Status:MEDLINE


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[PMID]:28744874
[Au] Autor:Broach JS; Ohs CL; Breen NE
[Ad] Endereço:Program of Fisheries and Aquatic Sciences, School of Forest Resources and Conservation, Institute of Food and Agricultural Sciences, University of Florida, 7922 NW 71st St, Gainesville, FL 32653, U.S.A.
[Ti] Título:Protracted volitional spawning of pinfish Lagodon rhomboides and changes in egg quality and fatty-acid composition throughout the spawning season.
[So] Source:J Fish Biol;91(3):806-817, 2017 Sep.
[Is] ISSN:1095-8649
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Spawning performance of pinfish Lagodon rhomboides without use of hormonal aids was monitored over an extended season. Nearly three million eggs were obtained from 75 spawns collected over a 90-day consecutive period from a single population of four brood fish (1M:1F). A mean ± s.d. batch fecundity of 30·27 ± 22·64 eggs g female was estimated with 98·0 ± 0·06% of the batch composed of floating eggs which were 1·04 ± 0·04 mm in diameter and 85·71 ± 27·59% fertile. Floating eggs successfully hatched 54·65 ± 29·13% of the time which yielded larvae that were 2·59 ± 0·24 mm in length. Fatty acids within floating eggs were largely represented by polyunsaturated fatty acids (45·30 ± 2·14% of total fatty acids) of which linoleic acid [(c18:2n-6cis) 3·49 ± 1·69% trifluoroacetic acid (TFA)] and docosahexaenoic acid (DHA) [(c22:6n-3) 28·47 ± 1·48% TFA] represented the majority of fatty acids for n-6 and n-3 polyunsaturated fatty acids, respectively. The strongest correlations between fatty acids and hatching success and larval survival to first feeding were observed for the DHA:EPA (eicosapentaenoic acid; c20:5n-3) ratio and total n-6 polyunsaturated fatty-acids levels, respectively. These data demonstrate potential for producers to rely on natural spawns for extensive egg production and provide a baseline for future development of natural spawning protocols of captive L. rhomboides.
[Mh] Termos MeSH primário: Óvulo/metabolismo
Perciformes/fisiologia
Comportamento Sexual Animal
[Mh] Termos MeSH secundário: Animais
Aquicultura
Ácidos Docosa-Hexaenoicos/metabolismo
Ácido Eicosapentaenoico/metabolismo
Ácidos Graxos Insaturados/metabolismo
Feminino
Óvulo/fisiologia
Estações do Ano
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fatty Acids, Unsaturated); 25167-62-8 (Docosahexaenoic Acids); AAN7QOV9EA (Eicosapentaenoic Acid)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE
[do] DOI:10.1111/jfb.13381


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[PMID]:29284035
[Au] Autor:Handegard NO; Tenningen M; Howarth K; Anders N; Rieucau G; Breen M
[Ad] Endereço:Marine Ecosystem Acoustics, Institute of Marine Research, Bergen, Norway.
[Ti] Título:Effects on schooling function in mackerel of sub-lethal capture related stressors: Crowding and hypoxia.
[So] Source:PLoS One;12(12):e0190259, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The selectivity of fishing gears with respect to fish species and size is important, both for fisheries management and fishing operations. Purse seining is an efficient, environmentally friendly fish capture methodology generally targeting single species aggregations, but once a fish school has been selected and surrounded by the seine, there is no selections for individual size, species or catch quantity. A common practice for evaluating the catch is to haul the seine to a point where physical samples or inspections of catch composition can be made. The release process is called slipping and may lead to mortality in the released fish. The objective of this study was to simulate a crowding situation and investigate how the behaviour was affected in response to increased fish density, decreased oxygen levels, or a combination of the two, and to see if there is a behavioural measure that can be used to set safe crowding limits. The experiment was conducted on Mackerel (Scomber scombrus) held in net pens. The volume of the net pen was reduced to increase fish density, and a tarpaulin bag was wrapped around the pen to reduce the oxygen levels. Oxygen, fish density and space occupancy was monitored during the experiment, and the behavioural reactions was assessed using an imaging sonar. The main result was that the schooling function, i.e. the response to a predator model, was significantly reduced during crowding but not in response to hypoxia. There were some indications of a slow recovery of the function post-treatment. We conclude that crowding causes behavioural responses that occur before densities that induce fish mortality. Consequently, there is a behavioural response that could be used as a proxy for setting safe crowding limits.
[Mh] Termos MeSH primário: Comportamento Animal
Perciformes/fisiologia
Estresse Fisiológico
[Mh] Termos MeSH secundário: Animais
Conservação dos Recursos Naturais
Aglomeração
Projetos Piloto
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171229
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190259


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[PMID]:29331479
[Au] Autor:Zhao C; Zhu W; Yin S; Cao Q; Zhang H; Wen X; Zhang G; Xie W; Chen S
[Ad] Endereço:College of Life Sciences, Key Laboratory of Biodiversity and Biotechnology of Jiangsu Province, Nanjing Normal University, Nanjing, Jiangsu 210023, China; Co-Innovation Center for Marine Bio-Industry Technology of Jiangsu Province, Lianyungang, Jiangsu 222005, China.
[Ti] Título:Molecular characterization and expression of Piwil1 and Piwil2 during gonadal development and treatment with HCG and LHRH-A in Odontobutis potamophila.
[So] Source:Gene;647:181-191, 2018 Mar 20.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Piwi proteins play an important regulatory role in germ cell division during gametogenesis and gonad development. In order to understand the function of Piwi genes in the reproductive process of the dark sleeper, we identified and characterized Piwil1 and Piwil2 from gonad tissue. The tissue distribution demonstrated that Piwils were highly expressed in the gonad of the dark sleeper. During gonad development, higher expression was observed in stage I of both the testes and ovaries than in subsequent stages at mRNA and protein levels. The results of immunohistochemistry demonstrated that Piwils were predominantly distributed in the spermatogonia, spermatocytes, and early oocytes. When treated with the HPG axis hormone (HCG and LHRH-A2), the expression of Piwils was significantly decreased in the testes and ovaries at mRNA and protein levels. All of these results indicated that Piwils play a vital role in gonad development and gametogenesis. Our findings provide valuable evidence to further clarify the underlying modulation mechanism of Piwils in teleosts.
[Mh] Termos MeSH primário: Proteínas Argonauta/genética
Gonadotropina Coriônica/farmacologia
Regulação da Expressão Gênica no Desenvolvimento/genética
Hormônio Liberador de Gonadotropina/farmacologia
Gônadas/efeitos dos fármacos
Perciformes/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Sequência de Bases
Gametogênese/efeitos dos fármacos
Gametogênese/genética
Regulação da Expressão Gênica no Desenvolvimento/efeitos dos fármacos
Masculino
Oócitos/efeitos dos fármacos
Filogenia
RNA Mensageiro/genética
Espermatócitos/efeitos dos fármacos
Espermatogônias/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Argonaute Proteins); 0 (Chorionic Gonadotropin); 0 (RNA, Messenger); 33515-09-2 (Gonadotropin-Releasing Hormone)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180209
[Lr] Data última revisão:
180209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180115
[St] Status:MEDLINE


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[PMID]:29329926
[Au] Autor:Li Y; Luo J; Xu Q; Hou Y; Jiang P; Sun Y; Lu H; Han B; Zhang J
[Ad] Endereço:Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources (Shanghai Ocean University), Ministry of Education, Shanghai 201306, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China.
[Ti] Título:Characterization of Selenoprotein P cDNA of the Antarctic toothfish Dissostichus mawsoni and its role under cold pressure.
[So] Source:Gene;647:150-156, 2018 Mar 20.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Our previous study using comparative genome analysis revealed a significant gene copy number gain of Dissostichus mawsoni selenoprotein P (Dm-SEPP) during the evolutionary radiation of Antarctic notothenioids, suggesting that Dm-SEPP contribute to this process, but the detailed structure and function of this gene product remain unclear. In the present study, the Dm-SEPP cDNA was cloned and characterized. The Dm-SEPP cDNA contains 17 selenocysteines (Sec) encoded by TGA codons and 2 typical SECIS elements located in the 3'-UTR. Evolutionary analysis of the Dm-SEPP gene revealed that it's closely related to the SEPP gene of zebrafish (Danio rerio), showing 51% amino acid similarity. Over-expression of Dm-SEPP could protect mammalian cells under cold pressure, probably via eliminating ROS. Further study showed an increase of endogenous SEPP in zebrafish ZF4 cells under cold pressure, and knockdown of SEPP decreased cell viability, accompanied with increased ROS. Our results suggested a protective role of Dm-SEPP in cold adaptation in Antarctic notothenioids.
[Mh] Termos MeSH primário: DNA Complementar/genética
Perciformes/genética
Selenoproteína P/genética
Estresse Fisiológico/genética
[Mh] Termos MeSH secundário: Regiões 3' não Traduzidas/genética
Animais
Regiões Antárticas
Temperatura Baixa
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (3' Untranslated Regions); 0 (DNA, Complementary); 0 (Selenoprotein P)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180209
[Lr] Data última revisão:
180209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180114
[St] Status:MEDLINE


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[PMID]:29304159
[Au] Autor:Brandner J; Cerwenka AF; Schliewen UK; Geist J
[Ad] Endereço:Wasserwirtschaftsamt Regensburg, Regensburg, Germany.
[Ti] Título:Invasion strategies in round goby (Neogobius melanostomus): Is bigger really better?
[So] Source:PLoS One;13(1):e0190777, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Few studies have systematically investigated mid- or long-term temporal changes of biological characteristics in invasive alien species considering the different phases of an invasion. We studied the invasion performance of one of the most invasive species worldwide, the round goby Neogobius melanostomus, from total absence over first occurrence until establishment from 2010 to 2015 in the upper Danube River. After an upstream movement of the invasion front of about 30 river km within four years, the pattern that round goby pioneering populations significantly differ from longer established ones has been confirmed: Pioneering populations at the invasion front comprised more females than males, and adult specimens with a larger body size compared to those at longer inhabited areas. On the population-level, the proportion of juveniles increased with time since invasion. The results of this study provide support for the previously postulated ´bigger is better´ and ´individual trait utility´ hypotheses explaining invasion success in round goby. Pioneering invaders with their greater exploratory behavior, highly adaptive phenotypic plasticity and increased competitive ability seem to act as prime emperors of new habitats, strongly following and benefiting from man-made river-bank structures.
[Mh] Termos MeSH primário: Espécies Introduzidas
Perciformes
Rios
[Mh] Termos MeSH secundário: Fatores Etários
Animais
Tamanho Corporal
Europa (Continente)
Comportamento Exploratório
Feminino
Masculino
Perciformes/anatomia & histologia
Fenótipo
Dinâmica Populacional
Fatores Sexuais
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180210
[Lr] Data última revisão:
180210
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180106
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190777


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[PMID]:28987567
[Au] Autor:Lusher AL; Hernandez-Milian G; Berrow S; Rogan E; O'Connor I
[Ad] Endereço:Marine and Freshwater Research Centre, Galway-Mayo Institute of Technology, Dublin Road, Galway, Ireland. Electronic address: amy.lusher@niva.no.
[Ti] Título:Incidence of marine debris in cetaceans stranded and bycaught in Ireland: Recent findings and a review of historical knowledge.
[So] Source:Environ Pollut;232:467-476, 2018 Jan.
[Is] ISSN:1873-6424
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Interactions between marine mammals and plastic debris have been the focus of studies for many years. Examples of interactions include entanglement in discarded fishing items or the presence of ingested debris in digestive tracts. Plastics, including microplastics, are a form of marine debris globally distributed in coastal areas, oceanic waters and deep seas. Cetaceans which strand along the coast present a unique opportunity to study interactions between animals with macro- and microplastics. A combination of novel techniques and a review of historical data was used to complete an extensive study of cetaceans interacting with marine debris within Irish waters. Of the 25 species of marine mammals reported in Irish waters, at least 19 species were reported stranded between 1990 and 2015 (n = 2934). Two hundred and forty-one of the stranded cetaceans presented signs of possible entanglement or interactions with fisheries. Of this number, 52.7% were positively identified as bycatch or as entangled in fisheries items, 26.6% were classified as mutilated and 20.7% could not be related to fisheries but showed signs of entanglement. In addition, 274 cetaceans were recorded as by-catch during observer programmes targeting albacore tuna. Post-mortem examinations were carried out on a total of 528 stranded and bycaught individuals and 45 (8.5%) had marine debris in their digestive tracts: 21 contained macrodebris, 21 contained microdebris and three had both macro- and microdebris. Forty percent of the ingested debris were fisheries related items. All 21 individuals investigated with the novel method for microplastics contained microplastics, composed of fibres (83.6%) and fragments (16.4%). Deep diving species presented more incidences of macrodebris ingestion but it was not possible to investigate this relationship to ecological habitat. More research on the plastic implications to higher trophic level organisms is required to understand the effects of these pollutants.
[Mh] Termos MeSH primário: Cetáceos
Monitoramento Ambiental
Conteúdo Gastrointestinal/química
Resíduos/análise
Poluentes da Água/análise
[Mh] Termos MeSH secundário: Animais
Ecossistema
Pesqueiros
Incidência
Irlanda
Oceanos e Mares
Perciformes
Plásticos/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plastics); 0 (Waste Products); 0 (Water Pollutants)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180123
[Lr] Data última revisão:
180123
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171009
[St] Status:MEDLINE


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[PMID]:29198193
[Au] Autor:Cai Z; Mai K; Ai Q
[Ad] Endereço:Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) & Key Laboratory of Mariculture (Ministry of Education),Ocean University of China,5 Yushan Road,Qingdao,Shandong 266003,People's Republic of China.
[Ti] Título:Regulation of hepatic lipid deposition by phospholipid in large yellow croaker.
[So] Source:Br J Nutr;118(12):999-1009, 2017 Dec.
[Is] ISSN:1475-2662
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Dietary phospholipid (PL) supplementation has been shown to reduce lipid accumulation in the tissues of farmed fish; however, the mechanisms underlying this effect are largely unknown. Thus, the present study was conducted to evaluate the potential impacts of PL on hepatic lipid metabolism both in vivo and in vitro. For in vivo study, four experimental diets - low lipid and low PL diet, as control diet (LL-LP diet, containing 12 % lipid and 1·5 % PL), low-lipid and high-PL diet (containing 12 % lipid and 8 % PL), high-lipid and low-PL diet (HL-LP diet, containing 20 % lipid and 1·5 % PL) and high-lipid and high-PL diet (HL-HP diet, containing 20 % lipid and 8 % PL) - were randomly allocated to four groups of large yellow croaker (Larimichthys crocea) (three cages per group) with similar initial body weight (approximately 8 g). For in vitro study, primary hepatocytes isolated from large yellow croaker were incubated either with graded levels of phosphatidylcholine (PC) (0-250 µm) or small interfering RNA (siRNA) for CTP: choline phosphate cytidylyltranferase α (CCTα) (siRNA-CCTα). Results showed that survival was independent of dietary treatments (P>0·05). Weight gain and feed efficiency in the HL-HP group were significantly higher than in the LL-LP and HL-LP groups (P<0·05). High level of dietary PL could markedly reduce abnormal hepatic lipid accumulation induced by the HL-LP diet (P<0·05). Similarly, compared with the corresponding controls, a significant decrease/increase in lipid content was observed in primary hepatocytes incubated with PC/siRNA-CCTα (P<0·05). High level of dietary PL reversed the HL-LP diet-induced increased levels of mRNA of fatty acid uptake and lipid synthesis related genes (P<0·05). In addition, High level of dietary PL markedly down-regulated the transcript levels of fatty acid oxidation-related genes and enhanced the transcript levels of VLDL assembly-related genes regardless of dietary lipid levels (P<0·05). Compared with corresponding controls, primary hepatocytes treated with PC showed significantly higher mRNA expression of lipid synthesis and VLDL assembly-related genes and lower mRNA expression of fatty acid oxidation-related genes, with hepatocytes treated with siRNA-CCTα exhibiting the opposite trend (P<0·05). In summary, these results demonstrated that high level of dietary PL might reverse the HL-LP diet-induced abnormal lipid accumulation in the liver through inhibiting fatty acid uptake and lipid synthesis, together with promoting the lipid export at the transcriptional level. Lipid export-promoting effect of PC was confirmed by in vitro studies. The present study showed for the first time that PL or PC could influence various metabolic pathways to regulate hepatic lipid deposition in fish at least at the transcriptional level.
[Mh] Termos MeSH primário: Dieta/veterinária
Metabolismo dos Lipídeos
Fígado/metabolismo
Perciformes/metabolismo
Fosfolipídeos/administração & dosagem
[Mh] Termos MeSH secundário: Ração Animal
Animais
Antígenos CD36/genética
Antígenos CD36/metabolismo
Células Cultivadas
Diacilglicerol O-Aciltransferase/genética
Diacilglicerol O-Aciltransferase/metabolismo
Ácido Graxo Sintases/genética
Ácido Graxo Sintases/metabolismo
Proteínas de Transporte de Ácido Graxo/genética
Proteínas de Transporte de Ácido Graxo/metabolismo
Proteínas de Ligação a Ácido Graxo/genética
Proteínas de Ligação a Ácido Graxo/metabolismo
Ácidos Graxos/metabolismo
Proteínas de Peixes/genética
Proteínas de Peixes/metabolismo
Hepatócitos/metabolismo
Lipase/genética
Lipase/metabolismo
Lipase Lipoproteica/genética
Lipase Lipoproteica/metabolismo
Fosfatidilcolinas/administração & dosagem
Proteína de Ligação a Elemento Regulador de Esterol 1/genética
Proteína de Ligação a Elemento Regulador de Esterol 1/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (CD36 Antigens); 0 (Fatty Acid Transport Proteins); 0 (Fatty Acid-Binding Proteins); 0 (Fatty Acids); 0 (Fish Proteins); 0 (Phosphatidylcholines); 0 (Phospholipids); 0 (Sterol Regulatory Element Binding Protein 1); EC 2.3.1.20 (Diacylglycerol O-Acyltransferase); EC 2.3.1.85 (Fatty Acid Synthases); EC 3.1.1.3 (Lipase); EC 3.1.1.34 (Lipoprotein Lipase)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171227
[Lr] Data última revisão:
171227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171205
[St] Status:MEDLINE
[do] DOI:10.1017/S000711451700294X



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