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[PMID]:29175483
[Au] Autor:White DP; Baumgarner BL; Watanabe WO; Alam MS; Kinsey ST
[Ad] Endereço:Department of Biology and Marine Biology, University of North Carolina Wilmington, Wilmington, NC 28403-5915, United States. Electronic address: dpwhite4@illinois.edu.
[Ti] Título:The effects of dietary ß-guanidinopropionic acid on growth and muscle fiber development in juvenile red porgy, Pagrus pagrus.
[So] Source:Comp Biochem Physiol B Biochem Mol Biol;216:48-58, 2018 Feb.
[Is] ISSN:1879-1107
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:ß-guanidinopropionic acid (ß-GPA) has been used in mammalian models to reduce intracellular phosphocreatine (PCr) concentration, which in turn lowers the energetic state of cells. This leads to changes in signaling pathways that attempt to re-establish energetic homeostasis. Changes in those pathways elicit effects similar to those of exercise such as changes in body and muscle growth, metabolism, endurance and health. Generally, exercise effects are beneficial to fish health and aquaculture, but inducing exercise in fishes can be impractical. Therefore, this study evaluated the potential use of supplemental ß-GPA to induce exercise-like effects in a rapidly growing juvenile teleost, the red porgy (Pagrus pagrus). We demonstrate for the first time that ß-GPA can be transported into teleost muscle fibers and is phosphorylated, and that this perturbs the intracellular energetic state of the cells, although to a lesser degree than typically seen in mammals. ß-GPA did not affect whole animal growth, nor did it influence skeletal muscle fiber size or myonuclear recruitment. There was, however, an increase in mitochondrial volume within myofibers in treated fish. GC/MS metabolomic analysis revealed shifts in amino acid composition of the musculature, putatively reflecting increases in connective tissue and decreases in protein synthesis that are associated with ß-GPA treatment. These results suggest that ß-GPA modestly affects fish muscle in a manner similar to that observed in mammals, and that ß-GPA may have application to aquaculture by providing a more practical means of generating some of the beneficial effects of exercise in fishes.
[Mh] Termos MeSH primário: Suplementos Nutricionais
Guanidinas/farmacologia
Fibras Musculares Esqueléticas/metabolismo
Propionatos/farmacologia
Dourada/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Animais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Guanidines); 0 (Propionates); UL1984YRKA (guanidinopropionic acid)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180210
[Lr] Data última revisão:
180210
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE


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[PMID]:29324758
[Au] Autor:Cuadros A; Basterretxea G; Cardona L; Cheminée A; Hidalgo M; Moranta J
[Ad] Endereço:Instituto Español de Oceanografía (IEO), Estació d'Investigació Jaume Ferrer, Maó, Spain.
[Ti] Título:Settlement and post-settlement survival rates of the white seabream (Diplodus sargus) in the western Mediterranean Sea.
[So] Source:PLoS One;13(1):e0190278, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Survival during the settlement window is a limiting variable for recruitment. The survival is believed to be strongly determined by biological interactions and sea conditions, however it has been poorly investigated. We examined the settlement patterns related to relevant biotic and abiotic factors (i.e. Density-dependence, wind stress, wave height and coastal current velocity) potentially determining post-settler survival rates of a coastal necto-benthic fish of wide distribution in the Mediterranean and eastern Atlantic, the white seabream (Diplodus sargus). An observational study of the demography of juveniles of this species was carried out at six coves in Menorca Island (Balearic Islands, western Mediterranean). Three of the coves were located in the northern and wind exposed coast, at the Northeast (NE) side; while the other three were found along the southern and sheltered coast, at the Southwest (SW) side of the island. The settlement period extended from early May to late June and maximum juvenile densities at the sampling sites varied between 5 and 11 ind. m-1 with maximum values observed in late May simultaneously occurring in the two coasts. Our analysis of juvenile survival, based on the interpretation of the observed patters using an individual based model (IBM), revealed two stages in the size-mortality relationships. An initial density-dependent stage was observed for juveniles up to 20 mm TL, followed by a density independent stage when other factors dominated the survival at sizes > 20 mm TL. No significant environmental effects were observed for the small size class (<20mm TL). Different significant environmental effects affecting NE and SW coves were observed for the medium (20-30mm TL) and large (>30mm TL) size class. In the NE, the wind stress consistently affected the density of fish of 20-30 mm and >30 mm TL with a dome-shape effect with higher densities at intermediate values of wind stress and negative effect at the extremes. The best models applied in the SW coves showed a significant non-linear negative effect on fish density that was also consistent for both groups 20-30 mm and >30 mm TL. Higher densities were observed at low values of wave height in the two groups. Because of these variations, the number of juveniles present at the end of the period was unrelated to their initial density and average survival varied among locations. In consequence, recruitment was (1) primarily limited by denso-dependient procedures at settlement stage, and (2) by sea conditions at post-settlement, where extreme wave conditions depleted juveniles. Accordingly, regional hydrodynamic conditions during the settlement season produced significant impacts on the juvenile densities depending on their size and with contrasted effects in respectto cove orientation. The similar strength in larval supply between coves, in addition to the similar mean phenology for settlers in the north and south of the Island, suggests that all fish may come from the same parental reproductive pool. These factors should be taken into account when assessing relationships between settlers, recruits and adults of white seabream.
[Mh] Termos MeSH primário: Ecossistema
Dourada/fisiologia
[Mh] Termos MeSH secundário: Animais
Mar Mediterrâneo
Taxa de Sobrevida
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180206
[Lr] Data última revisão:
180206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180112
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190278


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[PMID]:27775556
[Au] Autor:Ivorra E; Verdu S; Sánchez AJ; Grau R; Barat JM
[Ad] Endereço:Departamento de Ingeniería de Sistemas y Automática, Universidad Politècnica de València, Valencia 46022, Spain. euivmar@upvnet.upv.es.
[Ti] Título:Predicting Gilthead Sea Bream (Sparus aurata) Freshness by a Novel Combined Technique of 3D Imaging and SW-NIR Spectral Analysis.
[So] Source:Sensors (Basel);16(10), 2016 Oct 19.
[Is] ISSN:1424-8220
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:A technique that combines the spatial resolution of a 3D structured-light (SL) imaging system with the spectral analysis of a hyperspectral short-wave near infrared system was developed for freshness predictions of gilthead sea bream on the first storage days (Days 0-6). This novel approach allows the hyperspectral analysis of very specific fish areas, which provides more information for freshness estimations. The SL system obtains a 3D reconstruction of fish, and an automatic method locates gilthead's pupils and irises. Once these regions are positioned, the hyperspectral camera acquires spectral information and a multivariate statistical study is done. The best region is the pupil with an R² of 0.92 and an RMSE of 0.651 for predictions. We conclude that the combination of 3D technology with the hyperspectral analysis offers plenty of potential and is a very promising technique to non destructively predict gilthead freshness.
[Mh] Termos MeSH primário: Técnicas Biossensoriais/métodos
Conservação de Alimentos/métodos
Imagem Tridimensional/métodos
Dourada
Espectroscopia de Luz Próxima ao Infravermelho/métodos
[Mh] Termos MeSH secundário: Animais
Análise Multivariada
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


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[PMID]:29248578
[Au] Autor:Rosa J; Cox CJ; Cancela ML; Laizé V
[Ad] Endereço:Centre of Marine Sciences (CCMAR), University of Algarve, Faro, Portugal; PhD Program in Biomedical Sciences, Department of Biomedical Sciences and Medicine (DCBM), University of Algarve, Faro, Portugal.
[Ti] Título:Identification of a fish short-chain dehydrogenase/reductase associated with bone metabolism.
[So] Source:Gene;645:137-145, 2018 Mar 01.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Although human and mouse genetics have largely contributed to the better understanding of the mechanisms underlying skeletogenesis, much more remains to be uncovered. In this regard alternative and complementary systems have been sought and cell systems capable of in vitro calcification have been developed to study the mechanisms underlying bone formation. In gilthead seabream (Sparus aurata), a gene coding for an unknown protein that is strongly up-regulated during extracellular matrix (ECM) mineralization of a pre-osteoblast cell line was recently identified as a potentially important player in bone formation. In silico analysis of the deduced protein revealed the presence of domains typical of short-chain dehydrogenase/reductases (SDR). Closely related to carbonyl reductase 1, seabream protein belongs to a novel subfamily of SDR proteins with no orthologs in mammals. Analysis of gene expression by qPCR confirmed the strong up-regulation of sdr-like expression during in vitro mineralization but also revealed high expression levels in calcified tissues. A possible role for Sdr-like in osteoblast and bone metabolism was further evidenced through (i) the localization by in situ hybridization of sdr-like transcript in pre-osteoblasts of the operculum and (ii) the regulation of sdr-like gene transcription by Runx2 and retinoic acid receptor, two regulators of osteoblast differentiation and mineralization. Expression data also indicated a role for Sdr-like in gastrointestinal tract homeostasis and during gilthead seabream development at gastrulation and metamorphosis. This study reports a new subfamily of short-chain dehydrogenases/reductases in vertebrates and, for the first time, provides evidence of a role for SDRs in bone metabolism, osteoblast differentiation and/or tissue mineralization.
[Mh] Termos MeSH primário: Clonagem Molecular/métodos
Dourada/genética
Redutases-Desidrogenases de Cadeia Curta/genética
Redutases-Desidrogenases de Cadeia Curta/metabolismo
[Mh] Termos MeSH secundário: Animais
Osso e Ossos/citologia
Osso e Ossos/metabolismo
Calcificação Fisiológica
Diferenciação Celular
Linhagem Celular
Simulação por Computador
Matriz Extracelular/metabolismo
Proteínas de Peixes/genética
Proteínas de Peixes/metabolismo
Trato Gastrointestinal/metabolismo
Filogenia
Dourada/metabolismo
Regulação para Cima
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fish Proteins); EC 1.1.1.- (Short Chain Dehydrogenase-Reductases)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171218
[St] Status:MEDLINE


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[PMID]:29261652
[Au] Autor:Vélez EJ; Azizi S; Verheyden D; Salmerón C; Lutfi E; Sánchez-Moya A; Navarro I; Gutiérrez J; Capilla E
[Ad] Endereço:Departament de Biologia Cellular, Fisiologia i Immunologia, Facultat de Biologia, Universitat de Barcelona, Barcelona, Spain.
[Ti] Título:Proteolytic systems' expression during myogenesis and transcriptional regulation by amino acids in gilthead sea bream cultured muscle cells.
[So] Source:PLoS One;12(12):e0187339, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Proteolytic systems exert an important role in vertebrate muscle controlling protein turnover, recycling of amino acids (AA) or its use for energy production, as well as other functions like myogenesis. In fish, proteolytic systems are crucial for the relatively high muscle somatic index they possess, and because protein is the most important dietary component. Thus in this study, the molecular profile of proteolytic markers (calpains, cathepsins and ubiquitin-proteasome system (UbP) members) were analyzed during gilthead sea bream (Sparus aurata) myogenesis in vitro and under different AA treatments. The gene expression of calpains (capn1, capn3 and capns1b) decreased progressively during myogenesis together with the proteasome member n3; whereas capn2, capns1a, capns1b and ubiquitin (ub) remained stable. Contrarily, the cathepsin D (ctsd) paralogs and E3 ubiquitin ligases mafbx and murf1, showed a significant peak in gene expression at day 8 of culture that slightly decreased afterwards. Moreover, the protein expression analyzed for selected molecules presented in general the same profile of the mRNA levels, which was confirmed by correlation analysis. These data suggest that calpains seem to be more important during proliferation, while cathepsins and the UbP system appear to be required for myogenic differentiation. Concerning the transcriptional regulation by AA, the recovery of their levels after a short starvation period did not show effects on cathepsins expression, whereas it down-regulated the expression of capn3, capns1b, mafbx, murf1 and up-regulated n3. With regards to AA deficiencies, the major changes occurred at day 2, when leucine limitation suppressed ctsb and ctsl expression. Besides at the same time, both leucine and lysine deficiencies increased the expression of mafbx and murf1 and decreased that of n3. Overall, the opposite nutritional regulation observed, especially for the UbP members, points out an efficient and complementary role of these factors that could be useful in gilthead sea bream diets optimization.
[Mh] Termos MeSH primário: Aminoácidos/farmacologia
Regulação da Expressão Gênica
Desenvolvimento Muscular
Músculos/metabolismo
Transcrição Genética
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Proteínas Musculares/metabolismo
Músculos/citologia
Proteólise
Reação em Cadeia da Polimerase em Tempo Real
Dourada
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (Muscle Proteins)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171221
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0187339


  6 / 1346 MEDLINE  
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[PMID]:28965514
[Au] Autor:Turkmen S; Zamorano MJ; Fernández-Palacios H; Hernández-Cruz CM; Montero D; Robaina L; Izquierdo M
[Ad] Endereço:Aquaculture Research Group (GIA),Research Institute in Sustainable Aquaculture and Marine Conservation (IU-ECOAQUA),Universidad de Las Palmas de Gran Canaria,Crta. Taliarte s/n,35214 Telde,Spain.
[Ti] Título:Parental nutritional programming and a reminder during juvenile stage affect growth, lipid metabolism and utilisation in later developmental stages of a marine teleost, the gilthead sea bream (Sparus aurata).
[So] Source:Br J Nutr;118(7):500-512, 2017 Oct.
[Is] ISSN:1475-2662
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Nutrition during periconception and early development can modulate metabolic routes to prepare the offspring for adverse conditions through a process known as nutritional programming. In gilthead sea bream, replacement of fish oil (FO) with linseed oil (LO) in broodstock diets improves growth in the 4-month-old offspring challenged with low-FO and low-fishmeal (FM) diets for 1 month. The present study further investigated the effects of broodstock feeding on the same offspring when they were 16 months old and were challenged for a second time with the low-FM and low-FO diet for 2 months. The results showed that replacement of parental moderate-FO feeding with LO, combined with juvenile feeding at 4 months old with low-FM and low-FO diets, significantly (P<0·05) improved offspring growth and feed utilisation of low-FM/FO diets even when they were 16 months old: that is, when they were on the verge of their first reproductive season. Liver fatty acid composition was significantly affected by broodstock or reminder diets as well as by their interaction. Moreover, the reduction of long-chain PUFA and increase in α-linolenic acid and linoleic acid in broodstock diets lead to a significant down-regulation of hepatic lipoprotein lipase (P<0·001) and elongation of very long-chain fatty acids protein 6 (P<0·01). Besides, fatty acid desaturase 2 values were positively correlated to hepatic levels of 18 : 4n-3, 18 : 3n-6, 20 : 5n-3, 22 : 6n-3 and 22 : 5n-6. Thus, this study demonstrated the long-term nutritional programming of gilthead sea bream through broodstock feeding, the effect of feeding a 'reminder' diet during juvenile stages to improve utilisation of low-FM/FO diets and fish growth as well as the regulation of gene expression along the fish's life-cycle.
[Mh] Termos MeSH primário: Ração Animal/análise
Dieta/veterinária
Metabolismo dos Lipídeos
Dourada/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Animais
Regulação para Baixo
Ácidos Graxos Dessaturases/genética
Ácidos Graxos Dessaturases/metabolismo
Ácidos Graxos/administração & dosagem
Ácidos Graxos Insaturados/administração & dosagem
Óleos de Peixe/administração & dosagem
Ácido Linoleico/administração & dosagem
Óleo de Semente do Linho/administração & dosagem
Lipase Lipoproteica/genética
Lipase Lipoproteica/metabolismo
Fígado/metabolismo
Ácido alfa-Linolênico/administração & dosagem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fatty Acids); 0 (Fatty Acids, Unsaturated); 0 (Fish Oils); 0RBV727H71 (alpha-Linolenic Acid); 8001-26-1 (Linseed Oil); 9KJL21T0QJ (Linoleic Acid); EC 1.14.19.- (Fatty Acid Desaturases); EC 3.1.1.34 (Lipoprotein Lipase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171003
[St] Status:MEDLINE
[do] DOI:10.1017/S0007114517002434


  7 / 1346 MEDLINE  
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[PMID]:28961266
[Au] Autor:Magliozzi L; Almada F; Robalo J; Mollo E; Polese G; Gonçalves EJ; Felline S; Terlizzi A; D'Aniello B
[Ad] Endereço:Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Universita`del Salento, Lecce, Italy.
[Ti] Título:Cryptic effects of biological invasions: Reduction of the aggressive behaviour of a native fish under the influence of an "invasive" biomolecule.
[So] Source:PLoS One;12(9):e0185620, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The invasive green alga Caulerpa cylindracea has become an important component of the diet of the Mediterranean white seabream Diplodus sargus. As a consequence of this "exotic diet", the algal bisindolic alkaloid caulerpin accumulates in the fish tissues. Although the compound shows structural similarity to endogenous indolamines that modulate animal behaviour, the potential impact of caulerpin on fish behaviour still remains unexplored. In this report, behavioural experiments both on groups and on single fish responding towards a mirror were performed under different doses of dietary caulerpin. Differences between treated and control groups for each behaviour and for the overall aggressive pattern during the different experimental phases showed that the aggressiveness of D. sargus decreased with the administration of caulerpin. These results call the attention to a still unexplored potential ability of bioactive metabolites from marine invasive species, to alter the behaviour on native species, with putative negative effects on patterns of fish growth and population dynamics.
[Mh] Termos MeSH primário: Agressão
Comportamento Animal
Clorófitas
Espécies Introduzidas
Dourada/fisiologia
[Mh] Termos MeSH secundário: Animais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170930
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185620


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[PMID]:28739284
[Au] Autor:García-García M; Sánchez-Hernández M; García-Hernández MP; García-Ayala A; Chaves-Pozo E
[Ad] Endereço:Sección de Microscopía, Servicio de Apoyo a la Investigación Regional, Campus of International Excellence "Campus Mare Nostrum", University of Murcia, Murcia 30100, Spain.
[Ti] Título:Role of 5α-dihydrotestosterone in testicular development of gilthead seabream following finasteride administration.
[So] Source:J Steroid Biochem Mol Biol;174:48-55, 2017 Nov.
[Is] ISSN:1879-1220
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In teleosts, spermatogenesis is regulated by pituitary gonadotropins and sex steroids. 5α-dihydrotestosterone (DHT), derived from testosterone (T) through the action of 5α-reductase, has recently been suggested to play a physiologically important role in some fish species. In this study, gilthead seabream, Sparus aurata L., males received an implant of 1µgT/g body mass (bm) or vehicle alone and, 7days later, 1mg finasteride (FIN, an inhibitor of 5α-reductase)/kg bm or vehicle. Serum levels of T, 11-ketotestosterone (11KT), DHT and 17ß-estradiol (E ), and the mRNA levels of the main enzymes involved in their synthesis, were analysed. T promoted a transient increase in the serum levels of T, 11KT and E but a decrease in those of DHT at day 15 following T injection, in accordance with the up-regulation of mRNA levels of the enzymes involved in T transformation to 11KT (coding genes: cyp11b1 and hsd11b) and the down-regulation of mRNA levels of the enzyme responsible for T transformation to DHT (coding gene: srd5a). Interestingly, a similar effect was observed when FIN was injected. However, when fish were injected with T and FIN successively (T+FIN), control levels were not recovered at the end of the experimental period (28days). DHT seems to regulate E serum levels via the down-regulation of mRNA levels of aromatase (coding gene: cyp19a1a), which is needed for the transformation of T into E . The testis histology, together with the proliferative rates recorded upon T, FIN or T+FIN treatment, suggests that DHT is involved in the onset of the meiotic phase of spermatogenesis.
[Mh] Termos MeSH primário: 3-Oxo-5-alfa-Esteroide 4-Desidrogenase/genética
Inibidores de 5-alfa Redutase/farmacologia
Androgênios/farmacologia
Di-Hidrotestosterona/sangue
Finasterida/farmacologia
Testosterona/farmacologia
[Mh] Termos MeSH secundário: 11-beta-Hidroxiesteroide Desidrogenase Tipo 1/genética
Androgênios/sangue
Animais
Aromatase/genética
Estradiol/sangue
Proteínas de Peixes/genética
Masculino
Dourada
Esteroide 11-beta-Hidroxilase/genética
Testículo/efeitos dos fármacos
Testículo/crescimento & desenvolvimento
Testosterona/análogos & derivados
Testosterona/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (5-alpha Reductase Inhibitors); 0 (Androgens); 0 (Fish Proteins); 08J2K08A3Y (Dihydrotestosterone); 3XMK78S47O (Testosterone); 4TI98Z838E (Estradiol); 57GNO57U7G (Finasteride); EC 1.1.1.146 (11-beta-Hydroxysteroid Dehydrogenase Type 1); EC 1.14.14.1 (Aromatase); EC 1.14.15.4 (Steroid 11-beta-Hydroxylase); EC 1.3.99.5 (3-Oxo-5-alpha-Steroid 4-Dehydrogenase)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170726
[St] Status:MEDLINE


  9 / 1346 MEDLINE  
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[PMID]:28735742
[Au] Autor:Barreto A; Luis LG; Soares AMVM; Paíga P; Santos LHMLM; Delerue-Matos C; Hylland K; Loureiro S; Oliveira M
[Ad] Endereço:Department of Biology & CESAM, University of Aveiro, 3810-193 Aveiro, Portugal. Electronic address: abarreto@ua.pt.
[Ti] Título:Genotoxicity of gemfibrozil in the gilthead seabream (Sparus aurata).
[So] Source:Mutat Res;821:36-42, 2017 Sep.
[Is] ISSN:1873-135X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Widespread use of pharmaceuticals and suboptimal wastewater treatment have led to increased levels of these substances in aquatic ecosystems. Lipid-lowering drugs such as gemfibrozil, which are among the most abundant human pharmaceuticals in the environment, may have deleterious effects on aquatic organisms. We examined the genotoxicity of gemfibrozil in a fish species, the gilthead seabream (Sparus aurata), which is commercially important in southern Europe. Following 96-h waterborne exposure, molecular (erythrocyte DNA strand breaks) and cytogenetic (micronuclei and other nuclear abnormalities in cells) endpoints were measured. Gemfibrozil was positive in both endpoints, at environmentally relevant concentrations, a result that raises concerns about the potential genotoxic effects of the drug in recipient waters.
[Mh] Termos MeSH primário: Quebras de DNA
Eritrócitos/efeitos dos fármacos
Genfibrozila/toxicidade
Micronúcleos com Defeito Cromossômico/induzido quimicamente
Dourada/genética
Poluentes Químicos da Água/toxicidade
[Mh] Termos MeSH secundário: Animais
Ensaio Cometa
Relação Dose-Resposta a Droga
Eritrócitos/patologia
Testes para Micronúcleos
Dourada/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Water Pollutants, Chemical); Q8X02027X3 (Gemfibrozil)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170725
[St] Status:MEDLINE


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[PMID]:28668330
[Au] Autor:Jin M; Monroig Ó; Navarro JC; Tocher DR; Zhou QC
[Ad] Endereço:Laboratory of Fish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, China; Institute of Aquaculture, Faculty of Natural Sciences, University of Stirling, Stirling FK9 4LA, Scotland, UK; Mariculture Efficient Healthy Breeding Synergy and Innovation Center of Zhejiang, China.
[Ti] Título:Molecular and functional characterisation of two elovl4 elongases involved in the biosynthesis of very long-chain (>C ) polyunsaturated fatty acids in black seabream Acanthopagrus schlegelii.
[So] Source:Comp Biochem Physiol B Biochem Mol Biol;212:41-50, 2017 Oct.
[Is] ISSN:1879-1107
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Elongation of very long-chain fatty acid (Elovl) 4 proteins are important fatty acyl elongases that participate in the biosynthesis of long-chain (C ) and very long-chain (˃C ) polyunsaturated fatty acids (LC-PUFA and VLC-PUFA, respectively) in teleost fish, especially in marine species. Moreover, knowledge of Elovl4 and other elongases such as Elovl2 has contributed to an advanced understanding of the LC-PUFA biosynthetic pathway in marine fish. In the present study, elovl4a and elovl4b were cloned from black seabream Acanthopagrus schlegelii and functionally characterised using recombinant expression in yeast. The elovl4a and elovl4b cDNA sequences included open reading frames (ORF) of 969 and 918 base pairs (bp), encoding proteins of 322 and 315 amino acids (aa), respectively. The functional characterisation of A. schlegelii Elovl4 proteins showed they were able to utilise all assayed C PUFA substrates except 22:6n-3. Moreover, it was particularly noteworthy that both A. schlegelii Elovl4a and Elovl4b proteins had the ability to elongate 20:5n-3 and 22:5n-3 to 24:5n-3, which can be potentially desaturated and ß-oxidised to 22:6n-3. Tissue transcript abundance analysis showed the highest expression of elovl4a and elovl4b in brain and eye, respectively, suggesting these tissues were major sites for VLC-PUFA biosynthesis in black seabream. The functions of the A. schlegelii Elovl4-like elongases, Elovl4a and Elovl4b, characterised in the present study, along with those of the Elovl5 and fatty acyl desaturase (Fads2) proteins of A. schlegelii characterised previously, provided evidence of the biosynthetic pathways of LC-PUFA and VLC-PUFA in this teleost species.
[Mh] Termos MeSH primário: Acetiltransferases/genética
Acetiltransferases/metabolismo
Ácidos Graxos Insaturados/biossíntese
Proteínas de Peixes/genética
Proteínas de Peixes/metabolismo
Dourada/genética
Dourada/metabolismo
[Mh] Termos MeSH secundário: Acetiltransferases/química
Sequência de Aminoácidos
Animais
Sequência de Bases
Vias Biossintéticas
Clonagem Molecular
DNA Complementar/genética
Ácidos Graxos Insaturados/química
Proteínas de Peixes/química
Filogenia
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Saccharomyces cerevisiae/genética
Saccharomyces cerevisiae/metabolismo
Homologia de Sequência de Aminoácidos
Distribuição Tecidual
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Complementary); 0 (Fatty Acids, Unsaturated); 0 (Fish Proteins); 0 (Recombinant Proteins); EC 2.3.1.- (Acetyltransferases); EC 2.3.1.- (fatty acid elongases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170703
[St] Status:MEDLINE



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