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[PMID]:29339143
[Au] Autor:Komisarczuk AZ; Kongshaug H; Nilsen F
[Ad] Endereço:Sea Lice Research Centre, Department of Biology, University of Bergen, Thormøhlensgate 55, 5008, Bergen, Norway. Electronic address: Anna.Komisarczuk@uib.no.
[Ti] Título:Gene silencing reveals multiple functions of Na /K -ATPase in the salmon louse (Lepeophtheirus salmonis).
[So] Source:Exp Parasitol;185:79-91, 2018 Feb.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Na /K -ATPase has a key function in a variety of physiological processes including membrane excitability, osmoregulation, regulation of cell volume, and transport of nutrients. While knowledge about Na /K -ATPase function in osmoregulation in crustaceans is extensive, the role of this enzyme in other physiological and developmental processes is scarce. Here, we report characterization, transcriptional distribution and likely functions of the newly identified L. salmonis Na /K -ATPase (LsalNa /K -ATPase) α subunit in various developmental stages. The complete mRNA sequence was identified, with 3003 bp open reading frame encoding a putative protein of 1001 amino acids. Putative protein sequence of LsalNa /K -ATPase revealed all typical features of Na /K -ATPase and demonstrated high sequence identity to other invertebrate and vertebrate species. Quantitative RT-PCR analysis revealed higher LsalNa /K -ATPase transcript level in free-living stages in comparison to parasitic stages. In situ hybridization analysis of copepodids and adult lice revealed LsalNa /K -ATPase transcript localization in a wide variety of tissues such as nervous system, intestine, reproductive system, and subcuticular and glandular tissue. RNAi mediated knock-down of LsalNa /K -ATPase caused locomotion impairment, and affected reproduction and feeding. Morphological analysis of dsRNA treated animals revealed muscle degeneration in larval stages, severe changes in the oocyte formation and maturation in females and abnormalities in tegmental glands. Thus, the study represents an important foundation for further functional investigation and identification of physiological pathways in which Na /K -ATPase is directly or indirectly involved.
[Mh] Termos MeSH primário: Copépodes/enzimologia
Inativação Gênica
ATPase Trocadora de Sódio-Potássio/fisiologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Copépodes/genética
Copépodes/crescimento & desenvolvimento
Copépodes/fisiologia
DNA Complementar/química
Ectoparasitoses/parasitologia
Ectoparasitoses/veterinária
Feminino
Doenças dos Peixes/parasitologia
Pesqueiros
Regulação Enzimológica da Expressão Gênica
Técnicas de Silenciamento de Genes
Hibridização In Situ
Masculino
Fases de Leitura Aberta/genética
Filogenia
Interferência de RNA
RNA de Cadeia Dupla
RNA Mensageiro/química
Reação em Cadeia da Polimerase em Tempo Real
Salmo salar/parasitologia
Água do Mar
Alinhamento de Sequência
ATPase Trocadora de Sódio-Potássio/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Complementary); 0 (RNA, Double-Stranded); 0 (RNA, Messenger); EC 3.6.3.9 (Sodium-Potassium-Exchanging ATPase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180118
[St] Status:MEDLINE


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[PMID]:28467401
[Au] Autor:He X; Houde ALS; Pitcher TE; Heath DD
[Ad] Endereço:Great Lakes Institute for Environmental Research, University of Windsor, Windsor, Ontario, Canada.
[Ti] Título:Genetic architecture of gene transcription in two Atlantic salmon (Salmo salar) populations.
[So] Source:Heredity (Edinb);119(2):117-124, 2017 08.
[Is] ISSN:1365-2540
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Gene expression regulation has an important role in short-term acclimation and long-term adaptation to changing environments. However, the genetic architecture of gene expression has received much less attention than that of traditional phenotypic traits. In this study, we used a 5 × 5 full-factorial breeding design within each of two Atlantic salmon (Salmo salar) populations to characterize the genetic architecture of gene transcription. The two populations (LaHave and Sebago) are being used for reintroduction efforts into Lake Ontario, Canada. We used high-throughput quantitative real-time PCR to measure gene transcription levels for 22 genes in muscle tissue of Atlantic salmon fry. We tested for population differences in gene transcription and partitioned the transcription variance into additive genetic, non-additive genetic and maternal effects within each population. Interestingly, average additive genetic effects for gene transcription were smaller than those reported for traditional phenotypic traits in salmonids, suggesting that the evolutionary potential of gene transcription is lower than that of traditional traits. Contrary to expectations for early life stage traits, maternal effects were small. In general, the LaHave population had higher additive genetic effects for gene transcription than the Sebago population had, indicating that the LaHave fish have a higher adaptive potential to respond to the novel selection pressures associated with reintroduction into a novel environment. This study highlights not only the profound variation in gene transcription possible among salmonid populations but also the among-population variation in the underlying genetic architecture of such traits.
[Mh] Termos MeSH primário: Genética Populacional
Salmo salar/genética
Transcrição Genética
[Mh] Termos MeSH secundário: Animais
Cruzamento
Meio Ambiente
Regulação da Expressão Gênica
Ontário
Fenótipo
Reação em Cadeia da Polimerase em Tempo Real
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1708
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1038/hdy.2017.24


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[PMID]:27776996
[Au] Autor:Holm HJ; Skugor S; Bjelland AK; Radunovic S; Wadsworth S; Koppang EO; Evensen Ø
[Ad] Endereço:Faculty of Veterinary Medicine and Biosciences, Department of Basic Sciences and Aquatic Medicine, Sea Lice Research Centre, Norwegian University of Life Sciences, Oslo, Norway. Electronic address: Helle.Holm@nmbu.no.
[Ti] Título:Contrasting expression of immune genes in scaled and scaleless skin of Atlantic salmon infected with young stages of Lepeophtheirus salmonis.
[So] Source:Dev Comp Immunol;67:153-165, 2017 02.
[Is] ISSN:1879-0089
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Atlantic salmon skin tissues with and without scales were taken from two preferred sites of salmon louse (Lepeophtheirus salmonis) attachment, behind the dorsal fin (scaled) and from the top of the head (scaleless), respectively. Tissues were profiled by qPCR of 32 genes to study responses to copepodids, 4 days post infection (dpi), and during the moult of copepodids to the chalimus stage, at 8 dpi. Basal/constitutive differences were found for many immune-related genes between the two skin sites; e.g., mannose binding protein C was over 100 fold higher expressed in the scaled skin from the back in comparison to the skin without scales from the head. With lice-infection, at 4 dpi most genes in both tissues showed lower values than in the non-infected control. By 8 dpi, the majority of responses increased towards the control levels, including cytokines of Th1, Th17 and Th2 pathways. Immunohistochemistry of three immune factors revealed an even distribution of MHC class II positive cells throughout epidermis, including the top layer of keratinocytes, marked compartmentalization of Mx and CD8α cells close to stratum basale, and an increase in numbers of CD8α cells in response to infection. In conclusion, suppression of immune genes during the copepodid stage likely sets off a beneficial situation for the parasite. At the moult to chalimus stage 8 dpi, only few genes surpassed the non-infected control levels, including CD8α. The gene expression pattern was reflected in the increased number of CD8α expressing cells, thus revealing a relatively minor activation of skin T-cell defenses in Atlantic salmon in response to L. salmonis infection.
[Mh] Termos MeSH primário: Escamas de Animais/fisiologia
Copépodes/imunologia
Proteínas de Peixes/metabolismo
Infestações por Piolhos/imunologia
Lectina de Ligação a Manose/metabolismo
Salmo salar/imunologia
Pele/imunologia
[Mh] Termos MeSH secundário: Escamas de Animais/parasitologia
Animais
Células Cultivadas
Citocinas/metabolismo
Proteínas de Peixes/genética
Imunidade/genética
Infestações por Piolhos/genética
Estágios do Ciclo de Vida
Lectina de Ligação a Manose/genética
Salmo salar/parasitologia
Pele/parasitologia
Fenômenos Fisiológicos da Pele/genética
Células Th1/imunologia
Células Th17/imunologia
Células Th2/imunologia
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cytokines); 0 (Fish Proteins); 0 (MBL2 protein, human); 0 (Mannose-Binding Lectin)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:180210
[Lr] Data última revisão:
180210
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161026
[St] Status:MEDLINE


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[PMID]:28743463
[Au] Autor:Rivas-Aravena A; Muñoz P; Jorquera P; Diaz A; Reinoso C; González-Catrilelbún S; Sandino AM
[Ad] Endereço:Comisión Chilena de Energía Nuclear, Departamento de Aplicaciones Nucleares, Laboratorio de Radiobiología Celular y Molecular. Nueva Bilbao 12501, Las Condes, Santiago, Chile; Universidad San Sebastián, Facultad de Ciencias, Lota 2465, Providencia, Santiago, Chile. Electronic address: andrea.rivas@c
[Ti] Título:Study of RNA-A Initiation Translation of The Infectious Pancreatic Necrosis Virus.
[So] Source:Virus Res;240:121-129, 2017 08 15.
[Is] ISSN:1872-7492
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The infectious pancreatic necrosis virus (IPNV) is a salmonid pathogen that causes significant economic losses to the aquaculture industry. IPNV is a non-enveloped virus containing two uncapped and non-polyadenylated double strand RNA genomic segments, RNA-A and RNA-B. The viral protein Vpg is covalently attached to the 5' end of both segments. There is little knowledge about its viral cycle, particularly about the translation of the RNAs. Through experiments using mono and bicistronic reporters, in this work we show that the 120-nucleotide-long 5'-UTR of RNA-A contains an internal ribosome entry site (IRES) that functions efficiently both in vitro and in salmon cells. IRES activity is strongly dependent on temperature. Also, the IRES structure is confined to the 5'UTR and is not affected by the viral coding sequence. This is the first report of IRES activity in a fish virus and can give us tools to generate antivirals to attack the virus without affecting fish directly.
[Mh] Termos MeSH primário: Infecções por Birnaviridae/veterinária
Doenças dos Peixes/virologia
Vírus da Necrose Pancreática Infecciosa/genética
Biossíntese de Proteínas
RNA Viral/genética
[Mh] Termos MeSH secundário: Regiões 5' não Traduzidas
Animais
Infecções por Birnaviridae/virologia
Regulação Viral da Expressão Gênica
Vírus da Necrose Pancreática Infecciosa/química
Vírus da Necrose Pancreática Infecciosa/metabolismo
Sítios Internos de Entrada Ribossomal
Conformação de Ácido Nucleico
RNA Mensageiro/química
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
RNA Viral/química
RNA Viral/metabolismo
Ribossomos/genética
Ribossomos/metabolismo
Salmo salar
Proteínas Virais/genética
Proteínas Virais/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (5' Untranslated Regions); 0 (Internal Ribosome Entry Sites); 0 (RNA, Messenger); 0 (RNA, Viral); 0 (Viral Proteins)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180209
[Lr] Data última revisão:
180209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE


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[PMID]:29205039
[Au] Autor:Rodrigues DB; Chitchumroonchokchai C; Mariutti LRB; Mercadante AZ; Failla ML
[Ad] Endereço:Department of Food Science, Faculty of Food Engineering, University of Campinas , Campinas, São Paulo 13083-862, Brazil.
[Ti] Título:Comparison of Two Static in Vitro Digestion Methods for Screening the Bioaccessibility of Carotenoids in Fruits, Vegetables, and Animal Products.
[So] Source:J Agric Food Chem;65(51):11220-11228, 2017 Dec 27.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In vitro digestion methods are routinely used to assess the bioaccessibility of carotenoids and other dietary lipophilic compounds. Here, we compared the recovery of carotenoids and their efficiency of micellarization in digested fruits, vegetables, egg yolk, and salmon and also in mixed-vegetable salads with and without either egg yolk or salmon using the static INFOGEST method22 and the procedure of Failla et al.16 Carotenoid stability during the simulated digestion was ≥70%. The efficiencies of the partitioning of carotenoids into mixed micelles were similar when individual plant foods and salad meals were digested using the two static methods. Furthermore, the addition of cooked egg or salmon to vegetable salads increased the bioaccessibility of some carotenoids. Our findings showed that the two methods of in vitro digestion generated similar estimates of carotenoid retention and bioaccessibility for diverse foods.
[Mh] Termos MeSH primário: Carotenoides/metabolismo
Digestão
Ovos/análise
Produtos Pesqueiros/análise
Frutas/metabolismo
Técnicas In Vitro/métodos
Verduras/metabolismo
[Mh] Termos MeSH secundário: Animais
Disponibilidade Biológica
Salmo salar
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
36-88-4 (Carotenoids)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180108
[Lr] Data última revisão:
180108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171206
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b04854


  6 / 2443 MEDLINE  
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[PMID]:27777106
[Au] Autor:Pereira-Torres D; Gonçalves AT; Ulloa V; Martínez R; Carrasco H; Olea AF; Espinoza L; Gallardo-Escárate C; Astuya A
[Ad] Endereço:Laboratory of Cell Culture and Marine Genomics, Department of Oceanography and COPAS Sur-Austral, University of Concepción, Casilla 160-C, Concepción, Chile.
[Ti] Título:In vitro modulation of Drimys winteri bark extract and the active compound polygodial on Salmo salar immune genes after exposure to Saprolegnia parasitica.
[So] Source:Fish Shellfish Immunol;59:103-108, 2016 Dec.
[Is] ISSN:1095-9947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The rapid development of the aquaculture industry has global concerns with health management and control strategies to prevent and/or treat diseases and increase sustainability standards. Saprolegniosis is a disease caused by Saprolegnia parasitica, and is characterized by promoting an immunosuppression in the host. This study evaluated in vitro the extract and one active compound (polygodial) of Drimys winteri, a Chilean medicinal tree as a potential early immunostimulatory aid in Saprolegniosis control. Atlantic salmon (Salmo salar) head kidney cells (ASK-1) were incubated with both extract and pure polygodial before exposure to S. parasitica mycelium, and the expression of the immune-related genes interleukin 1ß (IL-1ß), interferon α (IFNα), and major histocompatibility complex II (MHCII) was evaluated. Both evidenced immunomodulatory capacities by increasing gene expressions. This immunomodulation related to a mitigatory action counteracting the immunosuppressing effects of S. parasitica. Despite that most immune-related genes were up-regulated, the down-regulation of MHCII, characteristic of S. parasitica infection, was lessened by pre-incubation with the compounds. This study provides the first insight on the potential of D. winteri bark extract as a possible immunomodulatory and defensive strategy against this oomycete infection in fish.
[Mh] Termos MeSH primário: Drimys/química
Doenças dos Peixes/imunologia
Infecção/veterinária
Extratos Vegetais/farmacologia
Salmo salar
Sesquiterpenos/farmacologia
[Mh] Termos MeSH secundário: Ração Animal/análise
Animais
Dieta/veterinária
Doenças dos Peixes/microbiologia
Infecção/imunologia
Infecção/microbiologia
Casca de Planta/química
Extratos Vegetais/química
Saprolegnia/fisiologia
Sesquiterpenos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Extracts); 0 (Sesquiterpenes); 5FAF7T66M7 (polygodial)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171203
[Lr] Data última revisão:
171203
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161106
[St] Status:MEDLINE


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[PMID]:28949966
[Au] Autor:Herath TK; Ashby AJ; Jayasuriya NS; Bron JE; Taylor JF; Adams A; Richards RH; Weidmann M; Ferguson HW; Taggart JB; Migaud H; Fordyce MJ; Thompson KD
[Ad] Endereço:Institute of Aquaculture, University of Stirling, Stirling, United Kingdom.
[Ti] Título:Impact of Salmonid alphavirus infection in diploid and triploid Atlantic salmon (Salmo salar L.) fry.
[So] Source:PLoS One;12(9):e0179192, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:With increasing interest in the use of triploid salmon in commercial aquaculture, gaining an understanding of how economically important pathogens affect triploid stocks is important. To compare the susceptibility of diploid and triploid Atlantic salmon (Salmo salar L.) to viral pathogens, fry were experimentally infected with Salmonid alphavirus sub-type 1 (SAV1), the aetiological agent of pancreas disease (PD) affecting Atlantic salmon aquaculture in Europe. Three groups of fry were exposed to the virus via different routes of infection: intraperitoneal injection (IP), bath immersion, or cohabitation (co-hab) and untreated fry were used as a control group. Mortalities commenced in the co-hab challenged diploid and triploid fish from 11 days post infection (dpi), and the experiment was terminated at 17 dpi. Both diploid and triploid IP challenged groups had similar levels of cumulative mortality at the end of the experimental period (41.1% and 38.9% respectively), and these were significantly higher (p < 0.01) than for the other challenge routes. A TaqMan-based quantitative PCR was used to assess SAV load in the heart, a main target organ of the virus, and also liver, which does not normally display any pathological changes during clinical infections, but exhibited severe degenerative lesions in the present study. The median viral RNA copy number was higher in diploid fish compared to triploid fish in both the heart and the liver of all three challenged groups. However, a significant statistical difference (p < 0.05) was only apparent in the liver of the co-hab groups. Diploid fry also displayed significantly higher levels of pancreatic and myocardial degeneration than triploids. This study showed that both diploid and triploid fry are susceptible to experimental SAV1 infection. The lower virus load seen in the triploids compared to the diploids may possibly be related to differences in cell metabolism between the two groups, however, further investigation is necessary to confirm this and also to assess the outcome of PD outbreaks in other developmental stages of the fish when maintained in commercial production systems.
[Mh] Termos MeSH primário: Infecções por Alphavirus/virologia
Diploide
Salmo salar/virologia
Triploidia
[Mh] Termos MeSH secundário: Alphavirus/genética
Animais
Aquicultura
Rim/patologia
Fígado/patologia
Músculo Esquelético/patologia
Miocárdio/patologia
Pâncreas/patologia
RNA Viral/análise
RNA Viral/genética
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Salmo salar/genética
Carga Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170927
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0179192


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[PMID]:28868602
[Au] Autor:Smart D
[Ad] Endereço:Department of Diving and Hyperbaric Medicine, Royal Hobart Hospital and School of Medicine, and Faculty of Health Sciences, University of Tasmania, GPO Box 1061, Hobart, Tasmania 7001, Australia, david.smart@ths.tas.gov.au.
[Ti] Título:Ruptured tympanic membrane from underwater impact with an Atlantic salmon, Salmo salar.
[So] Source:Diving Hyperb Med;47(3):201-202, 2017 Sep.
[Is] ISSN:1833-3516
[Cp] País de publicação:Australia
[La] Idioma:eng
[Ab] Resumo:An unusual case of tympanic membrane rupture in a diver due to impact from an Atlantic salmon, Salmo salar is described. The diver was treated conservatively and the injury fully healed by eight weeks after which the diver successfully returned to diving. The author contends that the appearances of such traumatic perforations differ from barotrauma in divers secondary to ear clearing problems on descent.
[Mh] Termos MeSH primário: Acidentes de Trabalho
Mergulho
Pesqueiros
Salmo salar
Perfuração da Membrana Timpânica/etiologia
Membrana Timpânica/lesões
[Mh] Termos MeSH secundário: Adulto
Animais
Seres Humanos
Masculino
Ruptura/diagnóstico por imagem
Ruptura/etiologia
Membrana Timpânica/diagnóstico por imagem
Perfuração da Membrana Timpânica/diagnóstico por imagem
Conduta Expectante
[Pt] Tipo de publicação:CASE REPORTS
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170905
[St] Status:MEDLINE


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[PMID]:28800825
[Au] Autor:Khazandi M; Deo P; Ferro S; Venter H; Pi H; Crabb S; Amorico T; Ogunniyi AD; Trott DJ
[Ad] Endereço:Australian Centre for Antimicrobial Resistance Ecology, School of Animal and Veterinary Sciences, The University of Adelaide, Roseworthy 5371, Australia.
[Ti] Título:Efficacy evaluation of a new water sanitizer for increasing the shelf life of Southern Australian King George Whiting and Tasmanian Atlantic Salmon fillets.
[So] Source:Food Microbiol;68:51-60, 2017 Dec.
[Is] ISSN:1095-9998
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The bacterial species and specific spoilage organisms associated with the Southern Australian King George Whiting (KGW) and Tasmanian Atlantic Salmon (TAS), and the efficacy of a HOCl-containing water-based sanitization product (Electro-Chemically Activated Solution, by ECAS4) in extending the shelf life of KGW and TAS fillets were evaluated. Fillets were washed with an ECAS4 solution containing either 45 ppm or 150 ppm of free chlorine and bacterial species enumerated on selective and non-selective media, followed by identification of pure isolates by 16 S rRNA gene sequencing. The dominant spoilage microbiota in KGW and TAS fillets stored at 4 ± 1 °C were Pseudomonas spp. and Shewanella spp. At either concentration, ECAS4 significantly reduced total bacterial load and specific spoilage organisms on KGW and TAS fillets (approx. 1-2 log colony-forming units) during storage and significantly extended the shelf life of the fillets by 2 and 4 days, respectively. The significant increase in shelf life and quality of fillets was corroborated by raw and cooked sensory evaluation. ECAS4 sanitization could have a significant impact on the overall food industry, translating into health and economic benefits through reduction of food spoilage bacteria and potentially, foodborne pathogens without many of the disadvantages of currently approved biocides.
[Mh] Termos MeSH primário: Bactérias/efeitos dos fármacos
Desinfetantes/farmacologia
Produtos Pesqueiros/microbiologia
Contaminação de Alimentos/prevenção & controle
Conservação de Alimentos/métodos
Conservantes de Alimentos/farmacologia
Salmo salar/microbiologia
[Mh] Termos MeSH secundário: Animais
Bactérias/crescimento & desenvolvimento
Bactérias/isolamento & purificação
Conservação de Alimentos/instrumentação
Armazenamento de Alimentos
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Disinfectants); 0 (Food Preservatives)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170813
[St] Status:MEDLINE


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[PMID]:28735572
[Au] Autor:Clarkson M; Migaud H; Metochis C; Vera LM; Leeming D; Tocher DR; Taylor JF
[Ad] Endereço:1Institute of Aquaculture, Faculty of Natural Sciences,University of Stirling,Stirling FK9 4LA,UK.
[Ti] Título:Early nutritional intervention can improve utilisation of vegetable-based diets in diploid and triploid Atlantic salmon (Salmo salar L.).
[So] Source:Br J Nutr;118(1):17-29, 2017 Jul.
[Is] ISSN:1475-2662
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The present study investigated nutritional programming in Atlantic salmon to improve utilisation of a vegetable-based diet. At first exogenous feeding, fry were fed either a marine-based diet (Diet Mstimulus, 80% fishmeal (FM)/4% fish oil (FO)) or a vegetable-based diet (Diet Vstimulus, 10% FM/0% FO) for 3 weeks. Subsequently, all fish were then fed under the same conditions with a commercial, marine-based, diet for 15 weeks and thereafter challenged with a second V diet (Diet Vchallenge, 10% FM/0% FO) for 6 weeks. Diploid and triploid siblings were run in parallel to examine ploidy effects. Growth performance, feed intake, nutrient utilisation and intestinal morphology were monitored. Fish initially given Diet Vstimulus (V-fish) showed 24 % higher growth rate and 23 % better feed efficiency compared with M-fish when later challenged with Diet Vchallenge. There was no difference in feed intake between nutritional histories, but increased nutrient retentions highlighted the improved utilisation of a V diet in V-fish. There were generally few significant effects of nutritional history or ploidy on enteritis scores in the distal intestine after the challenge phase as only V-triploids showed a significant increase (P<0·05) in total score. The data highlighted that the positive effects were most likely a result of nutritional programming and the ability to respond better when challenged later in life may be attributed to physiological and/or metabolic changes induced by the stimulus. This novel study showed the potential of nutritional programming to improve the use of plant raw material ingredients in feeds for Atlantic salmon.
[Mh] Termos MeSH primário: Fenômenos Fisiológicos da Nutrição Animal
Dieta
Estado Nutricional
Preparações de Plantas/farmacologia
Ploidias
Salmo salar
Verduras
[Mh] Termos MeSH secundário: Ração Animal
Animais
Animais Recém-Nascidos
Aquicultura
Diploide
Ingestão de Energia
Crescimento
Intestinos/efeitos dos fármacos
Triploidia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Preparations)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170901
[Lr] Data última revisão:
170901
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170725
[St] Status:MEDLINE
[do] DOI:10.1017/S0007114517001842



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