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[PMID]:29382830
[Au] Autor:Aramillo Irizar P; Schäuble S; Esser D; Groth M; Frahm C; Priebe S; Baumgart M; Hartmann N; Marthandan S; Menzel U; Müller J; Schmidt S; Ast V; Caliebe A; König R; Krawczak M; Ristow M; Schuster S; Cellerino A; Diekmann S; Englert C; Hemmerich P; Sühnel J; Guthke R; Witte OW; Platzer M; Ruppin E; Kaleta C
[Ad] Endereço:Research Group Medical Systems Biology, Institute of Experimental Medicine, Christian-Albrechts-University Kiel, D-24105, Kiel, Germany.
[Ti] Título:Transcriptomic alterations during ageing reflect the shift from cancer to degenerative diseases in the elderly.
[So] Source:Nat Commun;9(1):327, 2018 01 30.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Disease epidemiology during ageing shows a transition from cancer to degenerative chronic disorders as dominant contributors to mortality in the old. Nevertheless, it has remained unclear to what extent molecular signatures of ageing reflect this phenomenon. Here we report on the identification of a conserved transcriptomic signature of ageing based on gene expression data from four vertebrate species across four tissues. We find that ageing-associated transcriptomic changes follow trajectories similar to the transcriptional alterations observed in degenerative ageing diseases but are in opposite direction to the transcriptomic alterations observed in cancer. We confirm the existence of a similar antagonism on the genomic level, where a majority of shared risk alleles which increase the risk of cancer decrease the risk of chronic degenerative disorders and vice versa. These results reveal a fundamental trade-off between cancer and degenerative ageing diseases that sheds light on the pronounced shift in their epidemiology during ageing.
[Mh] Termos MeSH primário: Envelhecimento/genética
Doenças Cardiovasculares/genética
Diabetes Mellitus/genética
Neoplasias/genética
Doenças Neurodegenerativas/genética
Transcriptoma
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Idoso de 80 Anos ou mais
Envelhecimento/metabolismo
Envelhecimento/patologia
Animais
Encéfalo/crescimento & desenvolvimento
Encéfalo/metabolismo
Doenças Cardiovasculares/sangue
Doenças Cardiovasculares/patologia
Criança
Pré-Escolar
Doença Crônica
Diabetes Mellitus/sangue
Diabetes Mellitus/patologia
Fundulidae/genética
Fundulidae/crescimento & desenvolvimento
Fundulidae/metabolismo
Ontologia Genética
Genoma Humano
Seres Humanos
Lactente
Fígado/crescimento & desenvolvimento
Fígado/metabolismo
Camundongos
Meia-Idade
Anotação de Sequência Molecular
Neoplasias/metabolismo
Neoplasias/patologia
Doenças Neurodegenerativas/sangue
Doenças Neurodegenerativas/patologia
Pele/crescimento & desenvolvimento
Pele/metabolismo
Peixe-Zebra/genética
Peixe-Zebra/crescimento & desenvolvimento
Peixe-Zebra/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180307
[Lr] Data última revisão:
180307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180201
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02395-2


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[PMID]:28882485
[Au] Autor:Zaremba A; Miller DS; Fricker G
[Ad] Endereço:Institute of Pharmacy and Molecular Biotechnology, University of Heidelberg, 69120 Heidelberg, Germany; Mount Desert Island Biological Laboratory, Salisbury Cove, ME 04672, United States.
[Ti] Título:Zinc chloride rapidly stimulates efflux transporters in renal proximal tubules of killifish (Fundulus heteroclitus).
[So] Source:Toxicol Appl Pharmacol;334:88-99, 2017 Nov 01.
[Is] ISSN:1096-0333
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Multidrug resistance-related protein 2 (Mrp2) is an ATP-driven efflux pump at the luminal membrane in renal proximal tubules. It acts as detoxification mechanism by transporting xenobiotics and metabolic products into urine. The trace element zinc is essential for cellular growth, differentiation and survival. It modulates immune response and is used as dietary supplement. Here, we found that 0.1-10µM ZnCl rapidly stimulated transport of the Mrp2 probe substrate Texas Red (TR) in isolated killifish renal proximal tubules, which provide an established model system to measure efflux transporter activity by using fluorescent probe substrates, confocal microscopy and image analysis. This stimulation was insensitive to the translation inhibitor cycloheximide (CHX), but it was quickly reversed by removing ZnCl from the incubation medium. ZnCl -induced transport stimulation was abolished by inhibitors and antagonists of the endothelin receptor type B (ET )/nitric oxide synthase (NOS)/protein kinase C (PKC) pathway. Moreover, ZnCl -induced effects were blocked by inhibition of PKCα using Gö6976 and PKCα inhibitor peptide C2-4. Both the phosphatidylinositol 3-kinase (PI3K) inhibitor LY 294002 and the mammalian target of rapamycin (mTOR) inhibitor rapamycin abolished ZnCl -induced transport stimulation. Furthermore, the stimulating effects of ZnCl were blocked by GSK650394, an inhibitor of the downstream target serum- and glucocorticoid-inducible kinase 1 (SGK1). ZnCl also stimulated transport mediated by P-glycoprotein (P-gp) and Breast cancer resistance protein (Bcrp). This is the first report about zinc affecting efflux transporter activity and demonstrates that ZnCl triggers a suite of signaling events to evoke a rapid stimulation of ABC transporter-mediated efflux in killifish proximal tubules.
[Mh] Termos MeSH primário: Proteínas de Transporte/metabolismo
Cloretos/toxicidade
Regulação da Expressão Gênica/efeitos dos fármacos
Túbulos Renais Proximais/efeitos dos fármacos
Compostos de Zinco/toxicidade
[Mh] Termos MeSH secundário: Animais
Proteínas de Transporte/genética
Fundulidae
Proteínas Imediatamente Precoces/genética
Proteínas Imediatamente Precoces/metabolismo
Fosfatidilinositol 3-Quinases/genética
Fosfatidilinositol 3-Quinases/metabolismo
Proteína Quinase C-alfa/genética
Proteína Quinase C-alfa/metabolismo
Proteínas Serina-Treonina Quinases/genética
Proteínas Serina-Treonina Quinases/metabolismo
Transdução de Sinais
Serina-Treonina Quinases TOR/genética
Serina-Treonina Quinases TOR/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carrier Proteins); 0 (Chlorides); 0 (Immediate-Early Proteins); 0 (Zinc Compounds); 86Q357L16B (zinc chloride); EC 2.7.1.- (Phosphatidylinositol 3-Kinases); EC 2.7.1.1 (TOR Serine-Threonine Kinases); EC 2.7.11.1 (Protein-Serine-Threonine Kinases); EC 2.7.11.1 (serum-glucocorticoid regulated kinase); EC 2.7.11.13 (Protein Kinase C-alpha)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170909
[St] Status:MEDLINE


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[PMID]:28768928
[Au] Autor:Higa M; Takashima Y; Yokaryo H; Harie Y; Suzuka T; Ogihara K
[Ad] Endereço:Department of Chemistry, Biology, and Marine Science, Faculty of Science, University of the Ryukyus.
[Ti] Título:Naphthoquinone Derivatives from Diospyros maritima.
[So] Source:Chem Pharm Bull (Tokyo);65(8):739-745, 2017.
[Is] ISSN:1347-5223
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:From the chloroform extract of the fresh fruits of Diospyros maritima BLUME (Ebenaceae), five new naphthoquinone derivatives, 2,7'-dimethyl-2',3-bijuglone (27), 2,7'-dimethyl-3,3'-bijuglone (28), 2,7'-dimethyl-6,8'-bijuglone (29), 7,7'-dimethyl-3,3'-ethylidenebijuglone (30), and 2',7-dimethyl-3,6'-ethylidenebijuglone (31), were isolated, in addition to twenty-one known naphthoquinone derivatives: plumbagin (4), droserone (5), 2,3-epoxyplumbagin (8), 3,3'-biplumbagin (9), chitranone (10), 3,8'-biplumbagin (11), elliptinone (12), maritinone (13), isozeylanone (14), methylene-3,3'-biplumbagin (15), ethylidene-3,3'-biplumbagin (16), ethylidene-3,6'-biplumbagin (17), ethylidene-6,6'-biplumbagin (18), 7-methyl-ß-dihydrojuglone (19), 7-methyljuglone (20), 2,3-epoxy-7-methyljuglone (21), neodiospyrin (22), mamegakinone (23), ehretione (24), isoxylospyrin (25) and ß-dihydroplumbagin (26). The structures of the new compounds were established by spectral analysis. The quinones obtained from the chloroform extract of the fruits were compared with previously reported quinones obtained from ethanol extracts. The quinones in the fruits were categorized in three groups: quinones from ethanol extract only, quinones from chloroform extract only, and quinones from both extracts. The six naphthoquinones, 19-21, 25, 26, and 29, were examined for their ichthyotoxic activity and germination inhibitory activity. Quinones 19-21, 26, and 29 showed ichthyotoxic activity against Japanese killifish (Oryzias latipes var.) at 10 ppm; quinones 19 to 21 and 26 showed germination inhibitory activity toward lettuce seeds (Lactuca sativa L. var. Great Lakes) at 100 ppm.
[Mh] Termos MeSH primário: Diospyros/química
Naftoquinonas/isolamento & purificação
Naftoquinonas/farmacologia
[Mh] Termos MeSH secundário: Animais
Fundulidae
Estrutura Molecular
Naftoquinonas/química
Oryzias
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Naphthoquinones)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170829
[Lr] Data última revisão:
170829
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170804
[St] Status:MEDLINE
[do] DOI:10.1248/cpb.c17-00178


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[PMID]:28695255
[Au] Autor:Dubansky B; Rice CD; Barrois LF; Galvez F
[Ad] Endereço:Department of Biological Sciences, Developmental Integrative Biology Cluster, University of North Texas, 225B Life Sciences Building, Denton, TX, 76203, USA. bd@unt.edu.
[Ti] Título:Biomarkers of Aryl-hydrocarbon Receptor Activity in Gulf Killifish (Fundulus grandis) From Northern Gulf of Mexico Marshes Following the Deepwater Horizon Oil Spill.
[So] Source:Arch Environ Contam Toxicol;73(1):63-75, 2017 Jul.
[Is] ISSN:1432-0703
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Following the Deepwater Horizon oil spill, shorelines throughout the Barataria Basin of the northern Gulf of Mexico in Louisiana were heavily oiled for months with Macondo-252 oil, potentially impacting estuarine species. The Gulf killifish (Fundulus grandis) has been identified as a sentinel species for the study of site-specific effects of crude oil contamination on biological function. In November and December 2010, 4-5 months after the Macondo well was plugged and new oil was no longer spilling into the Gulf waters, Gulf killifish were collected across the Barataria Basin from 14 sites with varying degrees of oiling. Fish collected from oiled sites exhibited biological indications of exposure to oil, including increase in cytochrome P4501A (CYP1A) mRNA transcript and protein abundances in liver tissues. Immunohistochemistry revealed increases in gill, head kidney, and intestinal CYP1A protein at heavily oiled sites. Intestinal CYP1A protein was a sensitive indicator of exposure, indicating that intestinal tissue plays a key role in biotransformation of AHR ligands and that ingestion is a probable route of exposure, warranting additional consideration in future studies.
[Mh] Termos MeSH primário: Monitoramento Ambiental
Fundulidae/metabolismo
Poluição por Petróleo
Petróleo/toxicidade
Receptores de Hidrocarboneto Arílico/metabolismo
Poluentes Químicos da Água/toxicidade
[Mh] Termos MeSH secundário: Animais
Biomarcadores/metabolismo
Golfo do México
Zonas Úmidas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Petroleum); 0 (Receptors, Aryl Hydrocarbon); 0 (Water Pollutants, Chemical)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170719
[Lr] Data última revisão:
170719
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170712
[St] Status:MEDLINE
[do] DOI:10.1007/s00244-017-0417-6


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[PMID]:28609996
[Au] Autor:Ghedotti MJ; Davis MP
[Ad] Endereço:Regis University, Department of Biology, 3333 Regis Boulevard, Denver, Colorado 80221, USA.. mghedott@regis.edu.
[Ti] Título:The taxonomic placement of three fossil Fundulus species and the timing of divergence within the North American topminnows (Teleostei: Fundulidae).
[So] Source:Zootaxa;4250(6):577-586, 2017 Apr 10.
[Is] ISSN:1175-5334
[Cp] País de publicação:New Zealand
[La] Idioma:eng
[Ab] Resumo:The fossils species †Fundulus detillae, †F. lariversi, and †F. nevadensis from localities in the western United States are represented by well-preserved material with date estimations. We combined morphological data for these fossil taxa with morphological and DNA-sequence data to conduct a phylogenetic analysis and a tip-based divergence-time estimation for the family Fundulidae. The resultant phylogeny is largely concordant with the prior total-evidence phylogeny. The fossil species do not form a monophyletic group, and do not represent a discrete western radiation of Fundulus as previously proposed. The genus Fundulus diverged into subgeneric clades likely in the Eocene or Oligocene (mean age 34.6 mya, 53-23 mya), and all subgeneric and most species-group clades had evolved by the middle Miocene. †Fundulus lariversi is a member of subgenus Fundulus in which all extant species are found only in eastern North America, demonstrating that fundulids had a complicated biogeographic history. We confirmed †Fundulus detillae as a member of the subgenus Plancterus. †F. nevadensis is not classified in a subgenus but likely is related to the subgenera Plancterus and Wileyichthys.
[Mh] Termos MeSH primário: Fósseis
Fundulidae
Análise de Sequência de DNA
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
Evolução Molecular
Filogenia
Estados Unidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170809
[Lr] Data última revisão:
170809
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170615
[St] Status:MEDLINE
[do] DOI:10.11646/zootaxa.4250.6.5


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[PMID]:28589335
[Au] Autor:Oziolor EM; Carey AN; Matson CW
[Ad] Endereço:Department of Environmental Science, Center for Reservoir and Aquatic Systems Research (CRASR), Baylor University, Waco, TX, 76798, USA. elias_oziolor@baylor.edu.
[Ti] Título:A non-destructive BFCOD assay for in vivo measurement of cytochrome P450 3A (CYP3A) enzyme activity in fish embryos and larvae.
[So] Source:Ecotoxicology;26(6):809-819, 2017 Aug.
[Is] ISSN:1573-3017
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:There is increasing interest in quantifying the exposure and effects of anthropogenic contaminants in fish. Determination of exposures in wild fish is routinely performed, but methods to investigate potential effects are less established. One of the most relevant approaches would be the use of in vivo assays, but existing assays are often limited to in vitro determination of enzyme activity. Many pharmaceuticals and some persistent pollutants activate, and are metabolized by cytochrome P4503A (CYP3A), which make it a relevant and desirable target for biomarker research. We altered the established 7-benzyloxy-4-trifluoromethylcoumarin-O-debenzylation (BFCOD) in vitro protocol for CYP3A activity determination, developing a rapid and inexpensive method to measure in vivo (and in ovo) CYP3A activity in two fish systems: Gulf killifish (Fundulus grandis) and zebrafish (Danio rerio) early life stages. Even with very low concentrations of 7-benzyloxy-4-trifluoromethyl coumarin (BFC, 0.06 µM or 20 µg/L), we were able to detect significant induction in CYP3A activity in embryos of F. grandis, as well as in larvae of D. rerio in response to benzo[a]pyrene (BaP) and fluoranthene (FL) exposures. Because of concerns regarding the possible contribution of CYP1A to BFCOD activity from previous research, we have used a CYP1A post-translational inhibitor (FL) in order to calculate the contribution of CYP1A to the BFCOD assay. We also dosed with benzo[k]fluoranthene (BkF) and showed significant induction of CYP1A activity, with no concurrent increase in CYP3A activity. In this paper, we have taken an established in vitro CYP3A activity assay, and utilized the reaction in a novel way to allow for the non-destructive determination of CYP3A. In summary, we describe a sensitive, cheap, fast and easy modified BFCOD assay for in ovo and in vivo determination of CYP3A activity for use in moderate throughput early-life-stage fish experiments.
[Mh] Termos MeSH primário: Bioensaio/métodos
Citocromo P-450 CYP3A/metabolismo
Testes de Toxicidade/métodos
Poluentes Químicos da Água/toxicidade
[Mh] Termos MeSH secundário: Animais
Benzo(a)pireno/toxicidade
Fluorenos/toxicidade
Fundulidae/embriologia
Fundulidae/fisiologia
Hidrocarbonetos Aromáticos Policíclicos/toxicidade
Peixe-Zebra/embriologia
Peixe-Zebra/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fluorenes); 0 (Polycyclic Aromatic Hydrocarbons); 0 (Water Pollutants, Chemical); 3417WMA06D (Benzo(a)pyrene); 360UOL779Z (fluoranthene); EC 1.14.14.1 (Cytochrome P-450 CYP3A); U0P6LY48VF (benzo(k)fluoranthene)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170608
[St] Status:MEDLINE
[do] DOI:10.1007/s10646-017-1812-5


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[PMID]:28406214
[Au] Autor:Callaway E
[Ti] Título:'Young poo' makes aged fish live longer.
[So] Source:Nature;544(7649):147, 2017 04 04.
[Is] ISSN:1476-4687
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Envelhecimento
Transplante de Microbiota Fecal
Fezes/microbiologia
Fundulidae/microbiologia
Fundulidae/fisiologia
Longevidade
[Mh] Termos MeSH secundário: Envelhecimento/imunologia
Envelhecimento/fisiologia
Animais
Drosophila melanogaster
Fundulidae/imunologia
Trato Gastrointestinal/imunologia
Trato Gastrointestinal/microbiologia
Longevidade/imunologia
Longevidade/fisiologia
Camundongos
Modelos Biológicos
Rejuvenescimento
[Pt] Tipo de publicação:NEWS
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170822
[Lr] Data última revisão:
170822
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170414
[St] Status:MEDLINE
[do] DOI:10.1038/nature.2017.21770


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[PMID]:28362806
[Au] Autor:Baris TZ; Wagner DN; Dayan DI; Du X; Blier PU; Pichaud N; Oleksiak MF; Crawford DL
[Ad] Endereço:Marine Biology and Ecology, Rosenstiel School of Marine and Atmospheric Sciences, University of Miami, Rickenbacker Causeway, Miami, FL, United States of America.
[Ti] Título:Evolved genetic and phenotypic differences due to mitochondrial-nuclear interactions.
[So] Source:PLoS Genet;13(3):e1006517, 2017 Mar.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The oxidative phosphorylation (OxPhos) pathway is responsible for most aerobic ATP production and is the only pathway with both nuclear and mitochondrial encoded proteins. The importance of the interactions between these two genomes has recently received more attention because of their potential evolutionary effects and how they may affect human health and disease. In many different organisms, healthy nuclear and mitochondrial genome hybrids between species or among distant populations within a species affect fitness and OxPhos functions. However, what is less understood is whether these interactions impact individuals within a single natural population. The significance of this impact depends on the strength of selection for mito-nuclear interactions. We examined whether mito-nuclear interactions alter allele frequencies for ~11,000 nuclear SNPs within a single, natural Fundulus heteroclitus population containing two divergent mitochondrial haplotypes (mt-haplotypes). Between the two mt-haplotypes, there are significant nuclear allele frequency differences for 349 SNPs with a p-value of 1% (236 with 10% FDR). Unlike the rest of the genome, these 349 outlier SNPs form two groups associated with each mt-haplotype, with a minority of individuals having mixed ancestry. We use this mixed ancestry in combination with mt-haplotype as a polygenic factor to explain a significant fraction of the individual OxPhos variation. These data suggest that mito-nuclear interactions affect cardiac OxPhos function. The 349 outlier SNPs occur in genes involved in regulating metabolic processes but are not directly associated with the 79 nuclear OxPhos proteins. Therefore, we postulate that the evolution of mito-nuclear interactions affects OxPhos function by acting upstream of OxPhos.
[Mh] Termos MeSH primário: Núcleo Celular/genética
Evolução Molecular
Fundulidae/genética
Mitocôndrias/genética
[Mh] Termos MeSH secundário: Transporte Ativo do Núcleo Celular/genética
Animais
Núcleo Celular/metabolismo
DNA Mitocondrial/genética
DNA Mitocondrial/metabolismo
Proteínas de Peixes/genética
Proteínas de Peixes/metabolismo
Fundulidae/metabolismo
Frequência do Gene
Genética Populacional
Genótipo
Haplótipos
Desequilíbrio de Ligação
Mitocôndrias/metabolismo
Proteínas Mitocondriais/genética
Proteínas Mitocondriais/metabolismo
Proteínas Nucleares/genética
Proteínas Nucleares/metabolismo
Fosforilação Oxidativa
Fenótipo
Polimorfismo de Nucleotídeo Único
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Mitochondrial); 0 (Fish Proteins); 0 (Mitochondrial Proteins); 0 (Nuclear Proteins)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170401
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1006517


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[PMID]:28295034
[Au] Autor:Wagner DN; Baris TZ; Dayan DI; Du X; Oleksiak MF; Crawford DL
[Ad] Endereço:University of Miami, Rosenstiel School of Marine and Atmospheric Science, Miami, FL, USA.
[Ti] Título:Fine-scale genetic structure due to adaptive divergence among microhabitats.
[So] Source:Heredity (Edinb);118(6):594-604, 2017 Jun.
[Is] ISSN:1365-2540
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:It has been suggested that adaptive evolution on ecological timescales shapes communities. However, adaptation among environments relies on isolation or large selection coefficients that exceed migration effects. This reliance is tempered if adaptation is polygenic-does not depend on one allele completely replacing another but instead requires small allele frequency changes at many loci. Thus, whether individuals can evolve adaptation to fine-scale habitat variation (for example, microhabitats) is not resolved. Here we analyze the genetic divergence of the teleost fish, Fundulus heteroclitus, among microhabitats that are <200 m apart in three separate saltmarshes using 4741 single-nucleotide polymorphisms (SNPs). Among these SNPs, 1.3-2.3% have large and highly significant differences among microhabitats (mean F =0.15; false discovery rate ⩽1%). The divergence among microhabitats for these outlier SNPs is larger than that among populations, exceeds neutral expectation and indicates surprising population structure among microhabitats. Thus, we suggest that polygenic selection is surprisingly effective in altering allele frequencies among many different SNPs that share similar biological functions in response to environmental and ecological differences over very small geographic distances. We acknowledge the evolutionary difficulty of large genetic divergence among well-connected habitats. Therefore, these studies are only the first step to discern whether natural selection is responsible and capable of effecting genetic divergence on such a fine scale.
[Mh] Termos MeSH primário: Adaptação Fisiológica/genética
Evolução Biológica
Ecossistema
Fundulidae/genética
Genética Populacional
[Mh] Termos MeSH secundário: Alelos
Animais
Frequência do Gene
Genótipo
Desequilíbrio de Ligação
Modelos Genéticos
New Jersey
Polimorfismo de Nucleotídeo Único
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170629
[Lr] Data última revisão:
170629
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170316
[St] Status:MEDLINE
[do] DOI:10.1038/hdy.2017.6


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[PMID]:28259584
[Au] Autor:Oziolor EM; Howard W; Lavado R; Matson CW
[Ad] Endereço:Department of Environmental Science, Baylor University, Waco, TX, 76798, USA; Center for Reservoir and Aquatic Systems Research, Institute for Biomedical Studies, Baylor University, Waco, TX, 76798, USA. Electronic address: elias_oziolor@baylor.edu.
[Ti] Título:Induced pesticide tolerance results from detoxification pathway priming.
[So] Source:Environ Pollut;224:615-621, 2017 May.
[Is] ISSN:1873-6424
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Few studies in developmental toxicology have focused on whether early life contaminant exposure affects future susceptibility. Investigations in frogs suggested that early life exposure to a pesticide resulted in higher tolerance to a subsequent challenge. This led to the hypothesis that early-life stage exposures can alter phenotypically plastic traits during development, resulting in induced tolerance. Here, we used Gulf killifish (Fundulus grandis) to test the role of detoxification pathway priming in this inducible tolerance. In frogs, the induced tolerance is present five days after the end of the pre-exposure, but absent after a month. We show that a pre-exposure early in life with carbaryl, induces the activity of cytochrome P450 1A (CYP1A) and increases the ability of pre-exposed groups to metabolize carbaryl, likely because of activation of the aryl hydrocarbon receptor (AHR) pathway. Embryos pre-exposed to carbaryl had a 350-500% increase in CYP1A activity, threefold greater capacity to metabolize carbaryl and were more tolerant to a lethal challenge five days after the end of pre-exposure. However, ten days later the differences in CYP1A activity, metabolic capacity and tolerance between pre-exposed and control groups were no longer present. Thus, we conclude that the increase in tolerance observed in pre-exposed fish embryos was due to the activation of the AHR and other metabolic pathways, resulting in a prolonged increase in biotransformation capacity. This allowed individuals to more efficiently deal with subsequent chemical challenges for a short period after the initial pre-exposure. However, this induced tolerance was only short-lived due to the recycling of biotransformation enzymes in the cells as part of general cellular protein maintenance. These findings suggest that induced tolerance was likely due to induction of defense mechanisms during the duration of response to the original stressor, rather than a more permanent change in their ability to respond to future challenges.
[Mh] Termos MeSH primário: Carbaril/toxicidade
Fundulidae/metabolismo
Estágios do Ciclo de Vida/efeitos dos fármacos
Praguicidas/metabolismo
Poluentes Químicos da Água/metabolismo
Poluentes Químicos da Água/toxicidade
[Mh] Termos MeSH secundário: Animais
Texas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Pesticides); 0 (Water Pollutants, Chemical); R890C8J3N1 (Carbaryl)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170522
[Lr] Data última revisão:
170522
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170306
[St] Status:MEDLINE



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