Base de dados : MEDLINE
Pesquisa : B01.050.150.900.649.313.500.190.180 [Categoria DeCS]
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[PMID]:29484747
[Au] Autor:Hirad AH; Ahmad J; Alkhedhairy AA; Bahkali AH; Khan ST
[Ad] Endereço:Botany and Microbiology Department, College of Science, King Saud University, Riyadh, Saudi Arabia.
[Ti] Título:Bacterial isolates exhibiting multidrug resistance, hemolytic activity, and high 16S rRNA gene similarity with well-known pathogens found in camel milk samples of Riyadh region.
[So] Source:APMIS;126(3):215-226, 2018 Mar.
[Is] ISSN:1600-0463
[Cp] País de publicação:Denmark
[La] Idioma:eng
[Ab] Resumo:Customary consumption of unpasteurized milk by the population in the central Najed region of Saudi Arabia may pose a health risk. Therefore, 80 camel milk samples were collected aseptically from seven different stations of Riyadh region. The biochemical and microbiological properties of these milk samples were determined. Nutrient agar and brain heart infusion agar were used to determine mesophilic aerobic counts (MACs). The MAC in each mL of milk varied from 60 to 16 × 10  CFU/mL on nutrient agar. Based on the colony morphology, 176 colonies were collected from different samples, and these isolates were de-replicated into 80 unique isolates using rep-PCR analysis. Surprisingly, the 16S rRNA sequence analysis of these strains revealed that more than one-third of the collected milk samples contained strains that share maximum sequence similarities with well-known pathogens, such as Brucella, Bacillus anthracis, Listeria monocytogenes, and MRSA. Furthermore, many strains exhibit 16S rRNA gene similarity with opportunistic pathogens such as Citrobacter freundii and Kytococcus schroeteri. Many strains exhibit ß-hemolytic activity and resistant to six different antibiotics. Our study suggested that consumption of raw camel milk from this region constitutes a great health risk.
[Mh] Termos MeSH primário: Bactérias/isolamento & purificação
Leite/química
Leite/microbiologia
[Mh] Termos MeSH secundário: Animais
Bacillus anthracis/genética
Bacillus anthracis/isolamento & purificação
Bactérias/genética
Carga Bacteriana
Brucella/genética
Brucella/isolamento & purificação
Camelus
Citrobacter freundii/genética
Citrobacter freundii/isolamento & purificação
Microbiologia de Alimentos
Seres Humanos
Listeria monocytogenes/genética
Listeria monocytogenes/isolamento & purificação
Staphylococcus aureus Resistente à Meticilina/genética
Staphylococcus aureus Resistente à Meticilina/isolamento & purificação
Micrococcaceae/genética
Micrococcaceae/isolamento & purificação
Pasteurização
RNA Ribossômico 16S/genética
Arábia Saudita
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180228
[St] Status:MEDLINE
[do] DOI:10.1111/apm.12802


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[PMID]:29293334
[Au] Autor:Qiu Y; Li P; Dong S; Zhang X; Yang Q; Wang Y; Ge J; Hammock BD; Zhang C; Liu X
[Ad] Endereço:Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology,Key Laboratory of Control Technology and Standard for Agro-product Safety and Quality (Ministry of Agriculture), Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural
[Ti] Título:Phage-Mediated Competitive Chemiluminescent Immunoassay for Detecting Cry1Ab Toxin by Using an Anti-Idiotypic Camel Nanobody.
[So] Source:J Agric Food Chem;66(4):950-956, 2018 Jan 31.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cry toxins have been widely used in genetically modified organisms for pest control, raising public concern regarding their effects on the natural environment and food safety. In this work, a phage-mediated competitive chemiluminescent immunoassay (c-CLIA) was developed for determination of Cry1Ab toxin using anti-idiotypic camel nanobodies. By extracting RNA from camels' peripheral blood lymphocytes, a naive phage-displayed nanobody library was established. Using anti-Cry1Ab toxin monoclonal antibodies (mAbs) against the library for anti-idiotypic antibody screening, four anti-idiotypic nanobodies were selected and confirmed to be specific for anti-Cry1Ab mAb binding. Thereafter, a c-CLIA was developed for detection of Cry1Ab toxin based on anti-idiotypic camel nanobodies and employed for sample testing. The results revealed a half-inhibition concentration of developed assay to be 42.68 ± 2.54 ng/mL, in the linear range of 10.49-307.1 ng/mL. The established method is highly specific for Cry1Ab recognition, with negligible cross-reactivity for other Cry toxins. For spiked cereal samples, the recoveries of Cry1Ab toxin ranged from 77.4% to 127%, with coefficient of variation of less than 9%. This study demonstrated that the competitive format based on phage-displayed anti-idiotypic nanobodies can provide an alternative strategy for Cry toxin detection.
[Mh] Termos MeSH primário: Anticorpos Anti-Idiotípicos
Proteínas de Bactérias/análise
Bacteriófagos
Camelus/imunologia
Endotoxinas/análise
Proteínas Hemolisinas/análise
Medições Luminescentes/métodos
Anticorpos de Domínio Único
[Mh] Termos MeSH secundário: Animais
Anticorpos Monoclonais
Proteínas de Bactérias/imunologia
Camelus/sangue
Grãos Comestíveis/química
Endotoxinas/imunologia
Contaminação de Alimentos/análise
Proteínas Hemolisinas/imunologia
Linfócitos/química
Biblioteca de Peptídeos
RNA/isolamento & purificação
Anticorpos de Domínio Único/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Anti-Idiotypic); 0 (Antibodies, Monoclonal); 0 (Bacterial Proteins); 0 (Endotoxins); 0 (Hemolysin Proteins); 0 (Peptide Library); 0 (Single-Domain Antibodies); 0 (insecticidal crystal protein, Bacillus Thuringiensis); 63231-63-0 (RNA)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180103
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b04923


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[PMID]:28993122
[Au] Autor:Fukushi S; Fukuma A; Kurosu T; Watanabe S; Shimojima M; Shirato K; Iwata-Yoshikawa N; Nagata N; Ohnishi K; Ato M; Melaku SK; Sentsui H; Saijo M
[Ad] Endereço:Department of Virology I, National Institute of Infectious Diseases, Japan. Electronic address: fukushi@nih.go.jp.
[Ti] Título:Characterization of novel monoclonal antibodies against the MERS-coronavirus spike protein and their application in species-independent antibody detection by competitive ELISA.
[So] Source:J Virol Methods;251:22-29, 2018 Jan.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Since discovering the Middle East respiratory syndrome coronavirus (MERS-CoV) as a causative agent of severe respiratory illness in the Middle East in 2012, serological testing has been conducted to assess antibody responses in patients and to investigate the zoonotic reservoir of the virus. Although the virus neutralization test is the gold standard assay for MERS diagnosis and for investigating the zoonotic reservoir, it uses live virus and so must be performed in high containment laboratories. Competitive ELISA (cELISA), in which a labeled monoclonal antibody (MAb) competes with test serum antibodies for target epitopes, may be a suitable alternative because it detects antibodies in a species-independent manner. In this study, novel MAbs against the spike protein of MERS-CoV were produced and characterized. One of these MAbs was used to develop a cELISA. The cELISA detected MERS-CoV-specific antibodies in sera from MERS-CoV-infected rats and rabbits immunized with the spike protein of MERS-CoV. The MAb-based cELISA was validated using sera from Ethiopian dromedary camels. Relative to the neutralization test, the cELISA detected MERS-CoV-specific antibodies in 66 Ethiopian dromedary camels with a sensitivity and specificity of 98% and 100%, respectively. The cELISA and neutralization test results correlated well (Pearson's correlation coefficients=0.71-0.76, depending on the cELISA serum dilution). This cELISA may be useful for MERS epidemiological investigations on MERS-CoV infection.
[Mh] Termos MeSH primário: Anticorpos Monoclonais/sangue
Anticorpos Monoclonais/imunologia
Anticorpos Antivirais/imunologia
Ensaio de Imunoadsorção Enzimática/métodos
Coronavírus da Síndrome Respiratória do Oriente Médio/imunologia
Glicoproteína da Espícula de Coronavírus/imunologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Monoclonais/isolamento & purificação
Anticorpos Antivirais/isolamento & purificação
Camelus
Coelhos
Ratos
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Antibodies, Monoclonal); 0 (Antibodies, Viral); 0 (Spike Glycoprotein, Coronavirus)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180201
[Lr] Data última revisão:
180201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171011
[St] Status:MEDLINE


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[PMID]:29287083
[Au] Autor:Manee MM; Alharbi SN; Algarni AT; Alghamdi WM; Altammami MA; Alkhrayef MN; Alnafjan BM
[Ad] Endereço:National Center for Genomic Technology, King Abdulaziz City for Science and Technology, Riyadh, Saudi Arabia.
[Ti] Título:Molecular cloning, bioinformatics analysis, and expression of small heat shock protein beta-1 from Camelus dromedarius, Arabian camel.
[So] Source:PLoS One;12(12):e0189905, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Small heat shock protein beta-1 (HSPB-1) plays an essential role in the protection of cells against environmental stress.Elucidation of its molecular, structural, and biological characteristics in a naturally wild-type model is essential. Although the sequence information of the HSPB-1 gene is available for many mammalian species, the HSPB-1 gene of Arabian camel (Arabian camel HSPB-1) has not yet been structurally characterized. We cloned and functionally characterized a full-length of Arabian camel HSPB-1 cDNA. It is 791 bp long, with a 5'-untranslated region (UTR) of 34 bp, a 3'-UTR of 151 bp with a poly(A) tail, and an open reading frame (ORF) of 606 bp encoding a protein of 201 amino acids (accession number: MF278354). The tissue-specific expression analysis of Arabian camel HSPB-1 mRNA was examined using quantitative real-time PCR (qRT-PCR); which suggested that Arabian camel HSPB-1 mRNA was constitutionally expressed in all examined tissues of Arabian camel, with the predominately level in the esophagus tissue. Peptide mass fingerprint-mass spectrometry (PMF-MS) analysis of the purified Arabian camel HSPB-1 protein confirmed the identity of this protein. Phylogenetic analysis showed that the HSPB-1 protein of Arabian camel is grouped together with those of Bactrian camel and Alpaca. Comparing the modelled 3D structure of Arabian camel HSPB-1 protein with the available protein 3D structure of HSPB-1 from human confirmed the presence of α-crystallin domain, and high similarities were noted between the two structures by using super secondary structure prediction.
[Mh] Termos MeSH primário: Camelus/genética
Biologia Computacional
Proteínas de Choque Térmico/genética
[Mh] Termos MeSH secundário: Regiões 5' não Traduzidas
Sequência de Aminoácidos
Animais
Cromatografia Líquida
Clonagem Molecular
DNA Complementar/genética
Expressão Gênica
Proteínas de Choque Térmico/química
Modelos Moleculares
Filogenia
Estrutura Secundária de Proteína
RNA Mensageiro/genética
Reação em Cadeia da Polimerase em Tempo Real
Homologia de Sequência de Aminoácidos
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (5' Untranslated Regions); 0 (DNA, Complementary); 0 (Heat-Shock Proteins); 0 (RNA, Messenger)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171230
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189905


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[PMID]:28471361
[Au] Autor:Duhoo Y; Roche J; Trinh TTN; Desmyter A; Gaubert A; Kellenberger C; Cambillau C; Roussel A; Leone P
[Ad] Endereço:Centre National de la Recherche Scientifique, Architecture et Fonction des Macromolécules Biologiques, UMR 7257, Marseille, France.
[Ti] Título:Camelid nanobodies used as crystallization chaperones for different constructs of PorM, a component of the type IX secretion system from Porphyromonas gingivalis.
[So] Source:Acta Crystallogr F Struct Biol Commun;73(Pt 5):286-293, 2017 May 01.
[Is] ISSN:2053-230X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PorM is a membrane protein that is involved in the assembly of the type IX secretion system (T9SS) in Porphyromonas gingivalis, a major bacterial pathogen that is responsible for periodontal disease in humans. In the context of structural studies of PorM to better understand T9SS assembly, four camelid nanobodies were selected, produced and purified, and their specific interaction with the N-terminal or C-terminal part of the periplasmic domain of PorM was investigated. Diffracting crystals were also obtained, and the structures of the four nanobodies were solved by molecular replacement. Furthermore, two nanobodies were used as crystallization chaperones and turned out to be valuable tools in the structure-determination process of the periplasmic domain of PorM.
[Mh] Termos MeSH primário: Proteínas de Bactérias/química
Chaperonas Moleculares/química
Porphyromonas gingivalis/química
Anticorpos de Domínio Único/química
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Sistemas de Secreção Bacterianos/genética
Sistemas de Secreção Bacterianos/metabolismo
Sítios de Ligação
Camelídeos Americanos/imunologia
Camelus/imunologia
Clonagem Molecular
Cristalização
Cristalografia por Raios X
Escherichia coli/genética
Escherichia coli/metabolismo
Expressão Gênica
Vetores Genéticos/química
Vetores Genéticos/metabolismo
Cinética
Modelos Moleculares
Chaperonas Moleculares/biossíntese
Chaperonas Moleculares/isolamento & purificação
Biblioteca de Peptídeos
Porphyromonas gingivalis/metabolismo
Ligação Proteica
Conformação Proteica em alfa-Hélice
Conformação Proteica em Folha beta
Domínios e Motivos de Interação entre Proteínas
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Alinhamento de Sequência
Homologia de Sequência de Aminoácidos
Anticorpos de Domínio Único/biossíntese
Anticorpos de Domínio Único/isolamento & purificação
Termodinâmica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Bacterial Secretion Systems); 0 (Molecular Chaperones); 0 (Peptide Library); 0 (Recombinant Proteins); 0 (Single-Domain Antibodies)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171204
[Lr] Data última revisão:
171204
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1107/S2053230X17005969


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[PMID]:28946275
[Au] Autor:Lajnaf R; Picart-Palmade L; Cases E; Attia H; Marchesseau S; Ayadi MA
[Ad] Endereço:Alimentary Analysis Unit, National Engineering School of Sfax, BPW 3038 Sfax, Tunisia; Montpellier University, UMR IATE, Place E. Bataillon, 34095 Montpellier Cedex 5, France.
[Ti] Título:The foaming properties of camel and bovine whey: The impact of pH and heat treatment.
[So] Source:Food Chem;240:295-303, 2018 Feb 01.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The effect of heat treatment (70°C or 90°C for 30min) on the foaming and interfacial properties of acid and sweet whey obtained from bovine and camel fresh milk was examined. The maximum foamability and foam stability were observed for acid whey when compared to sweet whey for both milks, with higher values for the camel whey. This behavior for acid whey was explained by the proximity of the pI of whey protein (4.9-5.2), where proteins were found to carry the lowest negative charge as confirmed by the zeta potential measurements. Interfacial properties of acid camel whey and acid bovine whey were preserved at air water interface even after a heat treatment at 90°C. These results confirmed the pronounced foaming and interfacial properties of acid camel whey, even if acid and sweet bovine whey exhibited the highest viscoelastic modulus after heating.
[Mh] Termos MeSH primário: Camelus
Bovinos
Soro do Leite/química
[Mh] Termos MeSH secundário: Animais
Temperatura Alta
Concentração de Íons de Hidrogênio
Proteínas do Soro do Leite
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Whey Proteins)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170927
[St] Status:MEDLINE


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[PMID]:28873609
[Au] Autor:Ayyash M; Al-Nuaimi AK; Al-Mahadin S; Liu SQ
[Ad] Endereço:Food Science Department, College of Food and Agriculture, United Arab Emirates University (UAEU), PO Box 1555, Al Ain, United Arab Emirates. Electronic address: mutamed.ayyash@uaeu.ac.ae.
[Ti] Título:In vitro investigation of anticancer and ACE-inhibiting activity, α-amylase and α-glucosidase inhibition, and antioxidant activity of camel milk fermented with camel milk probiotic: A comparative study with fermented bovine milk.
[So] Source:Food Chem;239:588-597, 2018 Jan 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This study aimed to investigate in vitro the health-promoting benefits (anticancer activity, α-amylase and α-glucosidase inhibition, angiotensin-converting-enzyme (ACE)-inhibition, antioxidant and proteolytic activity) of camel milk fermented with indigenous probiotic strains of Lactobacillus spp., compared with fermented bovine milk. The three camel milk probiotic strains Lb. reuteri-KX881777, Lb. plantarum-KX881772, Lb. plantarum-KX881779 and a control strain Lb. plantarum DSM2468 were employed to ferment camel and bovine milks separately. The proteolytic and antioxidant activity of water soluble extracts (WSEs) from all fermented camel milks were higher than those of fermented bovine milk. α-Amylase inhibition of WSEs were >34% in both milk types fermented with all strains during storage periods, except the WSE of camel milk fermented by Lp.K772. The highest ACE-inhibition of the WSE from camel milk fermented by Lr.K777 was >80%. The proliferations of Caco-2, MCF-7 and HELA cells were more inhibited when treated with the WSE of fermented camel milk.
[Mh] Termos MeSH primário: Camelus
[Mh] Termos MeSH secundário: Animais
Antioxidantes
Células CACO-2
Fermentação
Seres Humanos
Leite
Probióticos
alfa-Amilases
alfa-Glucosidases
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); EC 3.2.1.1 (alpha-Amylases); EC 3.2.1.20 (alpha-Glucosidases)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE


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[PMID]:26363117
[Au] Autor:Saeed H; Ismaeil M; Embaby A; Ataya F; Malik A; Shalaby M; El-Banna S; Ali AAM; Bassiouny K
[Ad] Endereço:Department of Biotechnology, Institute of Graduate Studies and Research, Alexandria University, Alexandria, Egypt. Electronic address: hesham25166@hotmail.com.
[Ti] Título:Overexpression, purification and enzymatic characterization of a recombinant Arabian camel Camelus dromedarius glucose-6-phosphate dehydrogenase.
[So] Source:Protein Expr Purif;142:88-94, 2018 Feb.
[Is] ISSN:1096-0279
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In a previous study the full-length open reading frame of the Arabian camel, Camelus dromedarius liver cytosolic glucose-6-phosphate dehydrogenase (G6PD) cDNA was determined using reverse transcription polymerase chain reaction. The C. dromedarius cDNA was found to be 1545 nucleotides (accession number JN098421) that encodes a protein of 515 amino acids residues. In the present study, C. dromedarius recombinant G6PD was heterologously overexpressed in Escherichia coli BL21 (DE3) pLysS and purified by immobilized metal affinity fast protein liquid chromatography (FPLC) in a single step. The purity and molecular weight of the enzyme were analyzed on SDS-PAGE and the purified enzyme showed a single band on the gel with a molecular weight of 63.0 KDa. The specific activity was determined to be 2000 EU/mg protein. The optimum temperature and pH were found to be 60 °C and 7.4, respectively. The isoelectric point (pI) for the purified G6PD was determined to be 6.4. The apparent K values for the two substrates NADP and G6P were found to be 23.2 µM and 66.7 µM, respectively. The far-UV circular dichroism (CD) spectra of G6PD showed that it has two minima at 208 and 222 nm as well as maxima at 193 nm which is characteristic of high content of α-helix. Moreover, the far-UV CD spectra of the G6PD in the presence or absence of NADP were nearly identical.
[Mh] Termos MeSH primário: Glucose-6-Fosfato/química
Glucosefosfato Desidrogenase/metabolismo
NADP/química
Plasmídeos/química
[Mh] Termos MeSH secundário: Animais
Camelus
Clonagem Molecular
Ensaios Enzimáticos
Escherichia coli/genética
Escherichia coli/metabolismo
Expressão Gênica
Glucosefosfato Desidrogenase/genética
Concentração de Íons de Hidrogênio
Ponto Isoelétrico
Cinética
Fígado/química
Fígado/enzimologia
Peso Molecular
Plasmídeos/metabolismo
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Recombinant Proteins); 53-59-8 (NADP); 56-73-5 (Glucose-6-Phosphate); EC 1.1.1.49 (Glucosephosphate Dehydrogenase)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150913
[St] Status:MEDLINE


  9 / 3196 MEDLINE  
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[PMID]:28949782
[Au] Autor:Uversky VN; El-Fakharany EM; Abu-Serie MM; Almehdar HA; Redwan EM
[Ad] Endereço:a Department of Biological Sciences, Faculty of Sciences , King Abdulaziz University , Jeddah , Saudi Arabia.
[Ti] Título:Divergent Anticancer Activity of Free and Formulated Camel Milk α-Lactalbumin.
[So] Source:Cancer Invest;35(9):610-623, 2017 Oct 21.
[Is] ISSN:1532-4192
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Alpha-lactalbumin (α-LA), a small milk calcium-binding globular protein, is known to possess noticeable anticancer activity, which is determined by the ability of this protein to form complexes with oleic acid (OA). To date, in addition to human and bovine α-LA, the ability to form such anti-tumor complexes with OA was described for goat and camel α-LA. Although the mechanisms of the anticancer activity of human and bovine α-LA are already well-studied, little is currently known about the anticancer action of this camel protein. The goal of this study was to fill this gap and to analyze the anticancer and pro-apoptotic activities of camel α-LA in its free form (α-cLA) and as an OA-containing complex (OA-α-cLA) using four human cancer cell lines, including Caco-2 colon cancer cells, PC-3 prostate cancer cells, HepG-2 hepatoma cells, and MCF-7 breast cancer cells as targets. The anti-tumor activities of OA-α-cLA and α-cLA were analyzed using MTT test, annexin/PI staining, cell cycle analysis, nuclear staining, and tyrosine kinase (TK) inhibition methods. We show here that the OA-α-cLA complex does not affect normal cells but has noticeable anti-cancer activity, especially against MCF-7 cells, thus boosting the anticancer activity of α-cLA and improving the selectivity of OA. The OA-α-cLA complex mediated cancer cell death via selective induction of apoptosis and cell-cycle arrest at lower IC than that of free α-cLA by more than two folds. However, OA induced apoptosis at higher extent than OA-α-cLA and α-cLA. OA also caused unselective apoptosis-dependent cell death in both normal and cancer cells to a similar degree. The apoptosis and cell-cycle arresting effect of OA-α-cLA may be attributed to the TK inhibition activity of OA. Therefore, OA-α-cLA serves as efficient anticancer complex with two functional components, α-cLA and OA, possessing different activities. This study declared the effectiveness of OA-α-cLA complex as a promising entity with anticancer activity, and these formulated OA-camel protein complexes constitute an auspicious approach for cancer remedy, particularly for breast cancer.
[Mh] Termos MeSH primário: Antineoplásicos/farmacologia
Camelus
Lactalbumina/farmacologia
Leite/química
Neoplasias/tratamento farmacológico
Ácido Oleico/farmacologia
Inibidores de Proteínas Quinases/farmacologia
[Mh] Termos MeSH secundário: Animais
Antineoplásicos/toxicidade
Apoptose/efeitos dos fármacos
Células CACO-2
Pontos de Checagem do Ciclo Celular/efeitos dos fármacos
Proliferação Celular/efeitos dos fármacos
Cercopithecus aethiops
Composição de Medicamentos
Feminino
Células Hep G2
Seres Humanos
Lactalbumina/isolamento & purificação
Lactalbumina/toxicidade
Células MCF-7
Masculino
Neoplasias/enzimologia
Neoplasias/patologia
Ácido Oleico/toxicidade
Inibidores de Proteínas Quinases/toxicidade
Proteínas Tirosina Quinases/antagonistas & inibidores
Proteínas Tirosina Quinases/metabolismo
Células Vero
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Protein Kinase Inhibitors); 2UMI9U37CP (Oleic Acid); 9013-90-5 (Lactalbumin); EC 2.7.10.1 (Protein-Tyrosine Kinases)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170927
[St] Status:MEDLINE
[do] DOI:10.1080/07357907.2017.1373783


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[PMID]:28930753
[Au] Autor:Arab HH; Salama SA; Abdelghany TM; Omar HA; Arafa EA; Alrobaian MM; Maghrabi IA
[Ad] Endereço:Biochemistry Division and GTMR Unit, Department of Pharmacology and Toxicology, Faculty of Pharmacy, Taif University, Taif, Saudi Arabia.
[Ti] Título:Camel Milk Attenuates Rheumatoid Arthritis Via Inhibition of Mitogen Activated Protein Kinase Pathway.
[So] Source:Cell Physiol Biochem;43(2):540-552, 2017.
[Is] ISSN:1421-9778
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND/AIMS: Camel milk (CM) has shown beneficial anti-inflammatory actions in several experimental and clinical settings. So far, its effect on rheumatoid arthritis (RA) has not been previously explored. Thus, the current work aimed to evaluate the effects of CM in Adjuvant-induced arthritis and air pouch edema models in rats, which mimic human RA. METHODS: CM was administered at 10 ml/kg orally for 3 weeks starting on the day of Freund's adjuvant paw inoculation. The levels of TNF-α and IL-10 were measured by ELISA while the protein expression of NF-κBp65, COX-2 and iNOS was detected by immunohistochemistry. The expression of MAPK target proteins was assessed by Western blotting. RESULTS: CM attenuated paw edema, arthritic index and gait score along with dorsal pouch inflammatory cell migration. CM lowered the TNF-α and augmented the anti-inflammatory IL-10 levels in sera and exudates of arthritic rats. It also attenuated the expression of activated NF-κBp65, COX-2 and iNOS in the lining of the dorsal pouch. Notably, CM inhibited the MAPK pathway signal transduction via lowering the phosphorylation of p38 MAPK, ERK1/2 and JNK1/2 in rat hind paws. Additionally, CM administration lowered the lipid peroxide and nitric oxide levels and boosted glutathione and total anti-oxidant capacity in sera and exudates of animals. CONCLUSION: The observed CM downregulation of the arthritic process may support the interest of CM consumption as an adjunct approach for the management of RA.
[Mh] Termos MeSH primário: Anti-Inflamatórios/imunologia
Artrite Reumatoide/terapia
Leite/imunologia
Proteínas Quinases Ativadas por Mitógeno/imunologia
Transdução de Sinais
[Mh] Termos MeSH secundário: Animais
Artrite Experimental/imunologia
Artrite Experimental/patologia
Artrite Experimental/terapia
Artrite Reumatoide/imunologia
Artrite Reumatoide/patologia
Camelus/imunologia
Interleucina-10/análise
Interleucina-10/imunologia
Proteínas Quinases Ativadas por Mitógeno/análise
NF-kappa B/análise
NF-kappa B/imunologia
Óxido Nítrico Sintase Tipo II/análise
Óxido Nítrico Sintase Tipo II/imunologia
Ratos Wistar
Fator de Necrose Tumoral alfa/análise
Fator de Necrose Tumoral alfa/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (NF-kappa B); 0 (Tumor Necrosis Factor-alpha); 130068-27-8 (Interleukin-10); EC 1.14.13.39 (Nitric Oxide Synthase Type II); EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170921
[St] Status:MEDLINE
[do] DOI:10.1159/000480527



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