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[PMID]:29181905
[Au] Autor:Li GG; Zhang MX; Swa K; Maung KW; Quan RC
[Ad] Endereço:Southeast Asia Biodiversity Research Institute, Chinese Academy of Sciences, Yezin Nay Pyi Taw 05282, Myanmar.
[Ti] Título:Complete mitochondrial genome of the leaf muntjac ( ) and phylogenetics of the genus .
[So] Source:Zool Res;38(5):310-316, 2017 Sep 18.
[Is] ISSN:2095-8137
[Cp] País de publicação:China
[La] Idioma:eng
[Ab] Resumo:The leaf muntjac ( ) is an endemic deer species found in the east trans-Himalayan region. In recent years, population numbers have decreased due to heavy hunting and habitat loss, and little genetic data exists for this species, thus our knowledge of distribution rangs and population sizes likewise remain limited. We obtained mtDNA genes and the complete mitochondrial genome sequence of using PCR, followed by direct sequencing. The complete mitogenome sequence was determined as a circular 16 349 bp mitochondrial genome, containing 13 protein-coding genes, two rRNA genes, 22 tRNA genes, and one control region, the gene composition and order of which were similar to most other vertebrates so far reported. Most mitochondrial genes, except for and eight tRNAs, were encoded on the heavy strand. The overall base composition of the heavy strand was 33.1% A, 29.3% T, 24.2% C, and 13.4% G, with a strong AT bias of 62.4%. There were seven regions of gene overlap totaling 95 bp and 11 intergenic spacer regions totaling 74 bp. Phylogenetic analyses (ML and BI) among the genus based on the sequenced of mitogenome and supported as a member of , most closely related to . However, when analyses based on cyt included two more muntjacs, was most closely related to rather than , and together with , likely forming a complex of the species. This study will help in the exploration of the evolutionary history and taxonomic status of the leaf muntjac, as well as its protection as a genetic resource.
[Mh] Termos MeSH primário: DNA Mitocondrial/genética
Genoma Mitocondrial
Cervo Muntjac/genética
Filogenia
[Mh] Termos MeSH secundário: Animais
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Mitochondrial)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171129
[St] Status:MEDLINE
[do] DOI:10.24272/j.issn.2095-8137.2017.058


  2 / 127 MEDLINE  
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[PMID]:28539446
[Au] Autor:Nalls AV; McNulty E; Hoover CE; Pulscher LA; Hoover EA; Mathiason CK
[Ad] Endereço:Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, USA.
[Ti] Título:Infectious Prions in the Pregnancy Microenvironment of Chronic Wasting Disease-Infected Reeves' Muntjac Deer.
[So] Source:J Virol;91(15), 2017 Aug 01.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Ample evidence exists for the presence of infectious agents at the maternal-fetal interface, often with grave outcomes to the developing fetus (i.e., Zika virus, brucella, cytomegalovirus, and toxoplasma). While less studied, pregnancy-related transmissible spongiform encephalopathies (TSEs) have been implicated in several species, including humans. Our previous work has shown that prions can be transferred from mother to offspring, resulting in the development of clinical TSE disease in offspring born to muntjac dams infected with chronic wasting disease (CWD) (1). We further demonstrated protein misfolding cyclic amplification (PMCA)-competent prions within the female reproductive tract and in fetal tissues harvested from CWD experimentally and naturally exposed cervids (1, 2). To assess whether the PMCA-competent prions residing at the maternal-fetal interface were infectious and to determine if the real-time quaking-induced conversion (RT-QuIC) methodology may enhance our ability to detect amyloid fibrils within the pregnancy microenvironment, we employed a mouse bioassay and RT-QuIC. In this study, we have demonstrated RT-QuIC seeding activity in uterus, placentome, ovary, and amniotic fluid but not in allantoic fluids harvested from CWD-infected Reeves' muntjac dams showing clinical signs of infection (clinically CWD-infected) and in some placentomes from pre-clinically CWD-infected dams. Prion infectivity was confirmed within the uterus, amniotic fluid, and the placentome, the semipermeable interface that sustains the developing fetus, of CWD-infected dams. This is the first report of prion infectivity within the cervid pregnancy microenvironment, revealing a source of fetal CWD exposure prior to the birthing process, maternal grooming, or encounters with contaminated environments. The facile dissemination of chronic wasting disease within captive and free-range cervid populations has led to questions regarding the transmission dynamics of this disease. Direct contact with infected animals and indirect contact with infectious prions in bodily fluids and contaminated environments are suspected to explain the majority of this transmission. A third mode of transmission, from mother to offspring, may be underappreciated. The presence of pregnancy-related prion infectivity within the uterus, amniotic fluid, and the placental structure reveals that the developing fetus is exposed to a source of prions long before exposure to the infectious agent during and after the birthing process or via contact with contaminated environments. These findings have impact on our current concept of CWD disease transmission.
[Mh] Termos MeSH primário: Estruturas Animais/química
Cervo Muntjac
Complicações Infecciosas na Gravidez/veterinária
Príons/análise
Doença de Emaciação Crônica/patologia
[Mh] Termos MeSH secundário: Animais
Bioensaio
Técnicas de Química Analítica
Feminino
Camundongos
Gravidez
Complicações Infecciosas na Gravidez/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Prions)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171025
[Lr] Data última revisão:
171025
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170526
[St] Status:MEDLINE


  3 / 127 MEDLINE  
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[PMID]:28204896
[Au] Autor:McKillen J; Hogg K; Lagan P; Ball C; Doherty S; Reid N; Collins L; Dick JTA
[Ad] Endereço:Veterinary Sciences Division, Agri-Food and Biosciences Institute, Belfast, BT4 3SD, UK. john.mckillen@afbini.gov.uk.
[Ti] Título:Detection of a novel gammaherpesvirus (genus Rhadinovirus) in wild muntjac deer in Northern Ireland.
[So] Source:Arch Virol;162(6):1737-1740, 2017 Jun.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:This study represents the initial part of an investigation into the potential for non-native, wild, free-living muntjac deer (Muntiacus reevesi) to carry viruses that could be a threat to livestock. A degenerate PCR assay was used to screen a range of tissues from muntjac deer culled in Northern Ireland for the presence of herpesviral nucleic acids. This was followed by sequencing of PCR amplicons and phylogenetic analysis. We report the detection of a novel gammaherpesvirus most closely related to a type 2 ruminant rhadinovirus from mule deer. It remains to be determined if this new virus is pathogenic to deer or presents a risk to food security through the susceptibility of domestic livestock.
[Mh] Termos MeSH primário: Reservatórios de Doenças/veterinária
Rhadinovirus/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Animais Selvagens/virologia
Reservatórios de Doenças/virologia
Cervo Muntjac
Irlanda do Norte
Filogenia
Rhadinovirus/classificação
Rhadinovirus/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170630
[Lr] Data última revisão:
170630
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170217
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3254-z


  4 / 127 MEDLINE  
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[PMID]:28122497
[Au] Autor:Martins RF; Fickel J; Le M; van Nguyen T; Nguyen HM; Timmins R; Gan HM; Rovie-Ryan JJ; Lenz D; Förster DW; Wilting A
[Ad] Endereço:Leibniz Institute for Zoo and Wildlife Research (IZW), Alfred-Kowalke-Straße 17, 10315, Berlin, Germany. martins@izw-berlin.de.
[Ti] Título:Phylogeography of red muntjacs reveals three distinct mitochondrial lineages.
[So] Source:BMC Evol Biol;17(1):34, 2017 Jan 26.
[Is] ISSN:1471-2148
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The members of the genus Muntiacus are of particular interest to evolutionary biologists due to their extreme chromosomal rearrangements and the ongoing discussions about the number of living species. Red muntjacs have the largest distribution of all muntjacs and were formerly considered as one species. Karyotype differences led to the provisional split between the Southern Red Muntjac (Muntiacus muntjak) and the Northern Red Muntjac (M. vaginalis), but uncertainties remain as, so far, no phylogenetic study has been conducted. Here, we analysed whole mitochondrial genomes of 59 archival and 16 contemporaneous samples to resolve uncertainties about their taxonomy and used red muntjacs as model for understanding the evolutionary history of other species in Southeast Asia. RESULTS: We found three distinct matrilineal groups of red muntjacs: Sri Lankan red muntjacs (including the Western Ghats) diverged first from other muntjacs about 1.5 Mya; later northern red muntjacs (including North India and Indochina) and southern red muntjacs (Sundaland) split around 1.12 Mya. The diversification of red muntjacs into these three main lineages was likely promoted by two Pleistocene barriers: one through the Indian subcontinent and one separating the Indochinese and Sundaic red muntjacs. Interestingly, we found a high level of gene flow within the populations of northern and southern red muntjacs, indicating gene flow between populations in Indochina and dispersal of red muntjacs over the exposed Sunda Shelf during the Last Glacial Maximum. CONCLUSIONS: Our results provide new insights into the evolution of species in South and Southeast Asia as we found clear genetic differentiation in a widespread and generalist species, corresponding to two known biogeographical barriers: The Isthmus of Kra and the central Indian dry zone. In addition, our molecular data support either the delineation of three monotypic species or three subspecies, but more importantly these data highlight the conservation importance of the Sri Lankan/South Indian red muntjac.
[Mh] Termos MeSH primário: Mitocôndrias/genética
Cervo Muntjac/classificação
[Mh] Termos MeSH secundário: Animais
Ásia Sudeste
DNA Mitocondrial
Evolução Molecular
Índia
Cariotipagem
Tipagem Molecular
Cervo Muntjac/genética
Filogenia
Filogeografia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Mitochondrial)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170809
[Lr] Data última revisão:
170809
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170127
[St] Status:MEDLINE
[do] DOI:10.1186/s12862-017-0888-0


  5 / 127 MEDLINE  
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[PMID]:26959372
[Au] Autor:Schmid M; Steinlein C; Lomb C; Sperling K; Neitzel H
[Ad] Endereço:Department of Human Genetics, University of Wx00FC;rzburg, Wx00FC;rzburg, Germany.
[Ti] Título:5-Methylcytosine-Rich Heterochromatin in the Indian Muntjac.
[So] Source:Cytogenet Genome Res;147(4):240-6, 2015.
[Is] ISSN:1424-859X
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Two 5-methylcytosine (5-MeC)-rich heterochromatic regions were demonstrated in metaphase chromosomes of the Indian muntjac by indirect immunofluorescence using a monoclonal anti-5-MeC antibody. The metaphases were obtained from diploid and triploid cell lines. A major region is located in the 'neck' of the 3;X fusion chromosome and can be detected after denaturation of the chromosomal DNA with UV-light irradiation for 1 h. It is located exactly at the border of the X chromosome and the translocated autosome 3. A minor region is found in the centromeric region of the free autosome 3 after denaturing the chromosomal DNA for 3 h or longer. The structure and possible function of the major hypermethylated region as barrier against spreading of the X-inactivation process into the autosome 3 is discussed.
[Mh] Termos MeSH primário: 5-Metilcitosina/análise
Heterocromatina/química
Cervo Muntjac/genética
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Masculino
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Heterochromatin); 6R795CQT4H (5-Methylcytosine)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170223
[Lr] Data última revisão:
170223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160310
[St] Status:MEDLINE
[do] DOI:10.1159/000444431


  6 / 127 MEDLINE  
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[PMID]:25352187
[Au] Autor:Choi H; Wadduwage DN; Tu TY; Matsudaira P; So PT
[Ad] Endereço:Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139.
[Ti] Título:Three-dimensional image cytometer based on widefield structured light microscopy and high-speed remote depth scanning.
[So] Source:Cytometry A;87(1):49-60, 2015 Jan.
[Is] ISSN:1552-4930
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A high throughput 3D image cytometer have been developed that improves imaging speed by an order of magnitude over current technologies. This imaging speed improvement was realized by combining several key components. First, a depth-resolved image can be rapidly generated using a structured light reconstruction algorithm that requires only two wide field images, one with uniform illumination and the other with structured illumination. Second, depth scanning is implemented using the high speed remote depth scanning. Finally, the large field of view, high NA objective lens and the high pixelation, high frame rate sCMOS camera enable high resolution, high sensitivity imaging of a large cell population. This system can image at 800 cell/sec in 3D at submicron resolution corresponding to imaging 1 million cells in 20 min. The statistical accuracy of this instrument is verified by quantitatively measuring rare cell populations with ratio ranging from 1:1 to 1:10(5) . © 2014 International Society for Advancement of Cytometry.
[Mh] Termos MeSH primário: Algoritmos
Citometria por Imagem/instrumentação
Imagem Tridimensional/instrumentação
Microscopia/instrumentação
[Mh] Termos MeSH secundário: Animais
Fibroblastos/ultraestrutura
Corantes Fluorescentes
Citometria por Imagem/métodos
Imagem Tridimensional/métodos
Rim/ultraestrutura
Lentes
Luz
Iluminação
Camundongos
Microscopia/métodos
Cervo Muntjac
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Fluorescent Dyes)
[Em] Mês de entrada:1508
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141030
[St] Status:MEDLINE
[do] DOI:10.1002/cyto.a.22584


  7 / 127 MEDLINE  
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[PMID]:25079295
[Au] Autor:Willingham K; McNulty E; Anderson K; Hayes-Klug J; Nalls A; Mathiason C
[Ad] Endereço:Department of Microbiology, Immunology, and Pathology, Prion Research Center, Colorado State University; kassandrawillingham@gmail.com.
[Ti] Título:Milk collection methods for mice and Reeves' muntjac deer.
[So] Source:J Vis Exp;(89), 2014 Jul 19.
[Is] ISSN:1940-087X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Animal models are commonly used throughout research laboratories to accomplish what would normally be considered impractical in a pathogen's native host. Milk collection from animals allows scientists the opportunity to study many aspects of reproduction including vertical transmission, passive immunity, mammary gland biology, and lactation. Obtaining adequate volumes of milk for these studies is a challenging task, especially from small animal models. Here we illustrate an inexpensive and facile method for milk collection in mice and Reeves' muntjac deer that does not require specialized equipment or extensive training. This particular method requires two researchers: one to express the milk and to stabilize the animal, and one to collect the milk in an appropriate container from either a Muntjac or mouse model. The mouse model also requires the use of a P-200 pipetman and corresponding pipette tips. While this method is low cost and relatively easy to perform, researchers should be advised that anesthetizing the animal is required for optimal milk collection.
[Mh] Termos MeSH primário: Camundongos
Leite
Cervo Muntjac
Manejo de Espécimes/métodos
[Mh] Termos MeSH secundário: Animais
Camundongos Transgênicos
Modelos Animais
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; VIDEO-AUDIO MEDIA
[Em] Mês de entrada:1506
[Cu] Atualização por classe:161019
[Lr] Data última revisão:
161019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140801
[St] Status:MEDLINE
[do] DOI:10.3791/51007


  8 / 127 MEDLINE  
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[PMID]:24668678
[Au] Autor:Wang H; Luo X; Shi WB; Zhang BW
[Ad] Endereço:School of Life Science, Anhui University, Hefei, China.
[Ti] Título:Isolation and characterization of polymorphic microsatellite loci of the Chinese muntjac (Muntiacus reevesi).
[So] Source:Genet Mol Res;13(1):1905-8, 2014 Mar 17.
[Is] ISSN:1676-5680
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:Eight polymorphic microsatellite markers for Muntiacus reevesi were identified and characterized in this study. The number of alleles per locus ranged from 3 to 10 across 24-48 samples. The loci showed expected and observed heterozygosities of 0.577-0.876 and 0.387-0.933, respectively, with an average polymorphic information content value of 0.682. These markers should be a useful tool for further population and conservation genetic studies of Muntiacus reevesi.
[Mh] Termos MeSH primário: Repetições de Microssatélites/genética
Cervo Muntjac/genética
Polimorfismo Genético
[Mh] Termos MeSH secundário: Alelos
Animais
China
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1411
[Cu] Atualização por classe:140326
[Lr] Data última revisão:
140326
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140327
[St] Status:MEDLINE
[do] DOI:10.4238/2014.March.17.18


  9 / 127 MEDLINE  
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[PMID]:24430673
[Au] Autor:Walton KD; McNulty E; Nalls AV; Mathiason CK
[Ad] Endereço:Department of Microbiology, Immunology, and Pathology, Colorado State University.
[Ti] Título:Transabdominal ultrasound for pregnancy diagnosis in Reeves' muntjac deer.
[So] Source:J Vis Exp;(83):e50855, 2014 Jan 07.
[Is] ISSN:1940-087X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Reeves' muntjac deer (Muntiacus reevesi) are a small cervid species native to southeast Asia, and are currently being investigated as a potential model of prion disease transmission and pathogenesis. Vertical transmission is an area of interest among researchers studying infectious diseases, including prion disease, and these investigations require efficient methods for evaluating the effects of maternal infection on reproductive performance. Ultrasonographic examination is a well-established tool for diagnosing pregnancy and assessing fetal health in many animal species(1-7), including several species of farmed cervids(8-19), however this technique has not been described in Reeves' muntjac deer. Here we describe the application of transabdominal ultrasound to detect pregnancy in muntjac does and to evaluate fetal growth and development throughout the gestational period. Using this procedure, pregnant animals were identified as early as 35 days following doe-buck pairing and this was an effective means to safely monitor the pregnancy at regular intervals. Future goals of this work will include establishing normal fetal measurement references for estimation of gestational age, determining sensitivity and specificity of the technique for diagnosing pregnancy at various stages of gestation, and identifying variations in fetal growth and development under different experimental conditions.
[Mh] Termos MeSH primário: Cervo Muntjac/fisiologia
Prenhez/fisiologia
Ultrassonografia Pré-Natal/veterinária
[Mh] Termos MeSH secundário: Abdome/diagnóstico por imagem
Animais
Feminino
Gravidez
Ultrassonografia Pré-Natal/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; VIDEO-AUDIO MEDIA
[Em] Mês de entrada:1410
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140117
[St] Status:MEDLINE
[do] DOI:10.3791/50855


  10 / 127 MEDLINE  
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[PMID]:24220360
[Au] Autor:Lin CS; Pei KJ; Wang PS; Liu BT
[Ad] Endereço:Graduate Institute of Bioresources, National Pingtung University of Science and Technology, Neipu, Pingtung, Taiwan.
[Ti] Título:Evidence of higher levels of testosterone during the velvet period in muntjac than in other cervids.
[So] Source:Theriogenology;81(3):403-6, 2014 Feb.
[Is] ISSN:1879-3231
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Previous studies have shown that despite having a clear seasonal fluctuation in fecal testosterone concentration, the significantly lower testosterone levels found in velvet stags of the nonseasonal breeder muntjac (Muntiacus sp.) apparently did not stop their spermatogenesis as in other deer species. In the present study, in vitro cultivated Leydig cells isolated from adult stags of three native deer species of Taiwan were treated with androstenedione, with or without adding human chorionic gonadotropin. Results showed that, unlike the two seasonal breeders, sika deer (Cervus nippon) and sambar deer (Rusa unicolor), Leydig cells of velvet muntjac had no dramatic reduction in or even maintained the full capability of their testosterone productivity compared with the hard-antlered stage. The decrease in fecal testosterone level observed earlier in muntjac during the velvet period was probably due to a reduction of number of Leydig cells. These results support the hypothesis that testosterone production in muntjac during its velvet period might never be low enough to trigger the quiescent phase of the reproduction cycle.
[Mh] Termos MeSH primário: Cervo Muntjac/metabolismo
Testosterona/metabolismo
[Mh] Termos MeSH secundário: Androstenodiona/farmacologia
Animais
Gonadotropina Coriônica/farmacologia
Fezes/química
Células Intersticiais do Testículo/efeitos dos fármacos
Masculino
Cervo Muntjac/crescimento & desenvolvimento
Cervo Muntjac/fisiologia
Reprodução
Comportamento Sexual Animal
Espermatogênese
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Chorionic Gonadotropin); 3XMK78S47O (Testosterone); 409J2J96VR (Androstenedione)
[Em] Mês de entrada:1409
[Cu] Atualização por classe:140109
[Lr] Data última revisão:
140109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131114
[St] Status:MEDLINE



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