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Pesquisa : B01.050.150.900.649.313.500.880.399.800 [Categoria DeCS]
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[PMID]:29377941
[Au] Autor:Kunio M; Wong G; Markham PM; Edelman ER
[Ad] Endereço:Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America.
[Ti] Título:Sex differences in the outcomes of stent implantation in mini-swine model.
[So] Source:PLoS One;13(1):e0192004, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Sex-related differences have been noted in cardiovascular anatomy, pathophysiology, and treatment responses, yet we continued to drive evaluation of vascular device development in animal models without consideration of animal sex. We aimed to understand sex-related differences in the vascular responses to stent implantation by analyzing the pooled data of endovascular interventions in 164 Yucatan mini-swine (87 female, 77 male). Bare metal stents (BMS) or drug-eluting stents (DES) were implanted in 212 coronary arteries (63 single BMS implantation, 68 single DES implantation, 33 overlapped BMS implantation, and 48 overlapped DES implantation). Histomorphological parameters were evaluated from vascular specimens at 3-365 days after stent implantation and evaluated values were compared between female and male groups. While neointima formation at all times after implantation was invariant to sex, statistically significant differences between female and male groups were observed in injury, inflammation, adventitial fibrosis, and neointimal fibrin deposition. These differences were observed independently, i.e., for different procedure types and at different follow-up timings. Only subtle temporal sex-related differences were observed in extent and timing of resolution of inflammation and fibrin clearance. These subtle sex-related differences may be increasingly important as interventional devices meld novel materials that erode and innovations in drug delivery. Erodible materials may act differently if inflammation has a different temporal sequence with sex, and drug distribution after balloon or stent delivery might be different if the fibrin clearance speaks to different modes of pharmacokinetics in male and female swine.
[Mh] Termos MeSH primário: Modelos Animais
Fatores Sexuais
Stents
[Mh] Termos MeSH secundário: Animais
Feminino
Masculino
Suínos
Porco Miniatura
Túnica Íntima
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180130
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0192004


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[PMID]:29216295
[Au] Autor:Park J; Masaki T; Mezaki Y; Yokoyama H; Nakamura M; Maehashi H; Fujimi TJ; Gouraud SS; Nagatsuma K; Nakagomi M; Kimura N; Matsuura T
[Ad] Endereço:Department of Laboratory Medicine, The Jikei University School of Medicine, Minato-ku, Tokyo, Japan.
[Ti] Título:Alpha-1 antichymotrypsin is involved in astrocyte injury in concert with arginine-vasopressin during the development of acute hepatic encephalopathy.
[So] Source:PLoS One;12(12):e0189346, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND AND AIMS: We developed a bio-artificial liver (BAL) using a radial-flow bioreactor and rescued mini-pig models with lethal acute liver failure (ALF). The point of the rescue is the recovery from hepatic encephalopathy (HE). HE on ALF has sometimes resulted in brain death following brain edema with astrocyte swelling. Several factors, including ammonia and glutamine, have been reported to induce astrocyte swelling and injury. However, many clinicians believe that there are any other factors involved in the development of HE. Therefore, the aim of this study was to identify novel HE-inducible factors, particularly those inducing astrocyte dysfunction. METHODS: Mini-pig plasma samples were collected at three time points: before the administration of toxins (α-amanitin and LPS), when HE occurred after the administration of toxins, and after treatment with extracorporeal circulation (EC) by the BAL. To identify the causative factors of HE, each plasma sample was subjected to a comparative proteome analysis with two-dimensional gel electrophoresis and mass spectrometry. To assess the direct effects of candidate factors on the astrocyte function and injury, in vitro experiments with human astrocytes were performed. RESULTS: Using a proteome analysis, we identified alpha-1 antichymotrypsin (ACT), which was increased in plasma samples from mini-pigs with HE and decreased in those after treatment with EC by BAL. In in vitro experiments with human astrocytes, ACT showed growth-inhibitory and cytotoxic effects on astrocytes. In addition, the expression of water channel protein aquaporin-4, which is induced in injured astrocytes, was increased following ACT treatment. Interestingly, these effects of ACT were additively enhanced by adding arginine-vasopressin (AVP) and were canceled by adding an AVP receptor antagonist. CONCLUSIONS: These results suggest that ACT is involved in astrocyte injury and dysfunction in concert with AVP during the development of acute HE.
[Mh] Termos MeSH primário: Arginina Vasopressina/metabolismo
Astrócitos/metabolismo
Encefalopatia Hepática/metabolismo
alfa 1-Antiquimotripsina/farmacologia
[Mh] Termos MeSH secundário: Doença Aguda
Cloreto de Amônio/farmacologia
Animais
Astrócitos/efeitos dos fármacos
Linhagem Celular
Encefalopatia Hepática/patologia
Seres Humanos
Fígado Artificial
Masculino
Suínos
Porco Miniatura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (alpha 1-Antichymotrypsin); 01Q9PC255D (Ammonium Chloride); 113-79-1 (Arginine Vasopressin)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180122
[Lr] Data última revisão:
180122
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171208
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189346


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[PMID]:28463938
[Au] Autor:Clendenen N; Nunns GR; Moore EE; Reisz JA; Gonzalez E; Peltz E; Silliman CC; Fragoso M; Nemkov T; Wither MJ; Hansen K; Banerjee A; Moore HB; DʼAlessandro A
[Ad] Endereço:From the Department of Anesthesiology (N.C.), Department of Surgery (G.R.N., E.G., E.P., C.C.S., M.F., A.B., H.B.M.), University of Colorado Denver; Denver Health Hospital (E.E.M.); 4Department of Biochemistry and Molecular Genetics (J.A.R., T.N., T.N., M.J.W., A.D.A.), University of Colorado Denver; Bonfils Blood Center (C.C.S., K.H.,); and Department of Pediatrics (C.C.S.), University of Colorado Denver, Aurora, Colorado.
[Ti] Título:Hemorrhagic shock and tissue injury drive distinct plasma metabolome derangements in swine.
[So] Source:J Trauma Acute Care Surg;83(4):635-642, 2017 Oct.
[Is] ISSN:2163-0763
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Tissue injury and hemorrhagic shock induce significant systemic metabolic reprogramming in animal models and critically injured patients. Recent expansions of the classic concepts of metabolomic aberrations in tissue injury and hemorrhage opened the way for novel resuscitative interventions based on the observed abnormal metabolic demands. We hypothesize that metabolic demands and resulting metabolic signatures in pig plasma will vary in response to isolated or combined tissue injury and hemorrhagic shock. METHODS: A total of 20 pigs underwent either isolated tissue injury, hemorrhagic shock, or combined tissue injury and hemorrhagic shock referenced to a sham protocol (n = 5/group). Plasma samples were analyzed by UHPLC-MS. RESULTS: Hemorrhagic shock promoted a hypermetabolic state. Tissue injury alone dampened metabolic responses in comparison to sham and hemorrhagic shock, and attenuated the hypermetabolic state triggered by shock with respect to energy metabolism (glycolysis, glutaminolysis, and Krebs cycle). Tissue injury and hemorrhagic shock had a more pronounced effect on nitrogen metabolism (arginine, polyamines, and purine metabolism) than hemorrhagic shock alone. CONCLUSION: Isolated or combined tissue injury and hemorrhagic shock result in distinct plasma metabolic signatures. These findings indicate that optimized resuscitative interventions in critically ill patients are possible based on identifying the severity of tissue injury and hemorrhage.
[Mh] Termos MeSH primário: Metaboloma
Metabolômica/métodos
Choque Hemorrágico/sangue
Ferimentos e Lesões/sangue
[Mh] Termos MeSH secundário: Animais
Lesões por Esmagamento/sangue
Modelos Animais de Doenças
Metabolismo Energético
Fraturas do Fêmur/sangue
Intestinos/lesões
Masculino
Plasma
Suínos
Porco Miniatura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:180110
[Lr] Data última revisão:
180110
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1097/TA.0000000000001504


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[PMID]:29236794
[Au] Autor:Arruda EGP; Munhoz AM; Matsumoto W; Ueda T; Coudry RA; Gemperli R
[Ad] Endereço:Assistant Professor, Plastic Surgery Division, Department of Surgery, School of Medicine, Universidade de São Paulo (USP), Brazil. Conception and design of the study, acquisition and interpretation of data.
[Ti] Título:Qualitative analysis of the viability of autogenous fat grafts grafted in different environments of interstitial pressure. Preliminary results and description of a new experimental model in mini-pigs.
[So] Source:Acta Cir Bras;32(11):891-902, 2017 Nov.
[Is] ISSN:1678-2674
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:PURPOSE: To evaluate the feasibility of an experimental model of autologous fat graft (AFG) in different interstitial pressure (IP) environments. METHODS: Three mini-pigs(Minipig-BR) with age of 8 months (weight: 25-30 kg) were used. AFG were collected from the bucal fat pad, and grafted in the intramuscular pocket (biceps femoralis muscle). IP model was based on a fusiform ressection followed by primary closure "under tension". A blood pressure catheter located in the intramuscular region connected to a pressure module was applied to quantify IP. RESULTS: The mean operative time was 236 min (210 - 272 min). All the AFG and muscular segments were removed successfully. Average interstitial pressure CP and H were 3 and 10.6 mmHg respectively. The AFG were biopsied for histopathological analysis 30 days after graft. Hematoxylin-eosin staining and immunohistochemical analyzes (TNF-alpha, CD31 and Perilipine with monoclonal antibodies) were employed. CONCLUSION: The data show that minipigs model could be used as a recipient site for autologous fat graft techniques and allow the development of studies to explore the AFG intake and pathophysiology response.
[Mh] Termos MeSH primário: Tecido Adiposo/transplante
Modelos Animais de Doenças
Procedimentos Cirúrgicos Reconstrutivos/métodos
Transplante Autólogo/métodos
[Mh] Termos MeSH secundário: Animais
Estudos de Viabilidade
Sobrevivência de Enxerto
Imuno-Histoquímica
Masculino
Perilipinas/análise
Molécula-1 de Adesão Celular Endotelial de Plaquetas/análise
Pressão
Procedimentos Cirúrgicos Reconstrutivos/normas
Suínos
Porco Miniatura
Transplante Autólogo/normas
Fator de Necrose Tumoral alfa
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Perilipins); 0 (Platelet Endothelial Cell Adhesion Molecule-1); 0 (Tumor Necrosis Factor-alpha)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171220
[Lr] Data última revisão:
171220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE


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[PMID]:29180176
[Au] Autor:Vaena MLHT; Sinnecker JP; Vargas TJS; Serra-Guimarães F; Marques RG
[Ad] Endereço:Hospital Universitário Pedro Ernesto, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brazil. Electronic address: michel.vaena@hotmail.com.
[Ti] Título:Magnetic transcutaneous fixation: an experimental study in pigs.
[So] Source:J Surg Res;220:139-146, 2017 Dec.
[Is] ISSN:1095-8673
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Magnetic subdermal implants have never been studied in the context of magnetic fixation of an external device to the body's surface. Excessive attractive force between the implant and the external device may compromise local circulation due to mechanical compression, leading to necrosis. OBJECTIVE: To evaluate the feasibility of transcutaneous magnetic fixation and assess secondary skin changes when subjected to a continuous static magnetic field. METHODS: Using the pig as an animal model, 72 implants were introduced in 12 animals. After wound healing, ultrasonography was performed to measure implant depths. Computer simulations were applied to allow magnetic attachment between implants and external devices without impairing local blood flow. External devices of different magnetic strengths were applied over the skin for 7 days. Local skin was examined and collected for analysis. A senior dermatopathologist blindly examined skin specimens and controls for abnormal findings, measuring dermal and epidermal thickness. Statistical analysis (P <0.05) was performed over the data. RESULTS: Nineteen implants presented extrusion. The remaining 53 skin sites underwent magnetic compression, of which 43 (81%) evolved uneventfully. Implant depth varied between 4.6 mm and 8.3 mm (5.8 mm; ± 8.6 mm) with estimated pressure levels between 13.28 mmHg and 37.04 mmHg (27.6 mmHg; ±6.0 mmHg). Stronger magnets were associated with an increase in dermal thickness (P = 0.011) and neovascularization (P = 0.045). CONCLUSIONS: Transcutaneous magnetic fixation is compatible with skin viability in vivo, under experimental conditions. Skin interposition between two permanent magnets resulted in a continuous static magnetic field stimulation, which showed similar effects to pulsed electromagnetic fields reported on scientific literature.
[Mh] Termos MeSH primário: Imãs/efeitos adversos
Neovascularização Fisiológica
Próteses e Implantes/efeitos adversos
Pele/patologia
Estresse Mecânico
[Mh] Termos MeSH secundário: Animais
Estudos de Viabilidade
Campos Magnéticos/efeitos adversos
Masculino
Modelos Animais
Necrose/etiologia
Pele/irrigação sanguínea
Pele/diagnóstico por imagem
Suínos
Porco Miniatura
Ultrassonografia
Cicatrização
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171211
[Lr] Data última revisão:
171211
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171129
[St] Status:MEDLINE


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[PMID]:27773723
[Au] Autor:Langston JL; Myers TM
[Ad] Endereço:Analytical Toxicology Division, United States Army Medical Research Institute of Chemical Defense, Aberdeen Proving Ground, MD, USA.
[Ti] Título:VX toxicity in the Göttingen minipig.
[So] Source:Toxicol Lett;264:12-19, 2016 Dec 15.
[Is] ISSN:1879-3169
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The present experiments determined the intramuscular LD of VX in male Göttingen minipigs at two stages of development. In pubertal animals (115 days old), the LD of VX was indeterminate, but approximated 33.3µg/kg. However, in sexually mature animals (152 days old), the LD was estimated to be only 17.4µg/kg. Signs of nerve agent toxicity in the Göttingen minipig were similar to those described for other species, with some notable exceptions (such as urticaria and ejaculation). Latencies to the onset of sustained convulsions were inversely related to the administered dose of VX in both ages of minipigs. Additionally, actigraphy was used to quantify the presence of tremor and convulsions and, in some cases, was useful for precisely estimating time of death. The main finding indicates that in minipigs, as in other species, even relatively small differences in age can substantially alter the toxicity of nerve agents. Additionally, actigraphy can serve as a non-invasive method of characterizing the tremors and convulsions that often accompany nerve agent intoxication.
[Mh] Termos MeSH primário: Substâncias para a Guerra Química/toxicidade
Compostos Organotiofosforados/toxicidade
[Mh] Termos MeSH secundário: Envelhecimento
Animais
Injeções Intramusculares
Dose Letal Mediana
Masculino
Atividade Motora/efeitos dos fármacos
Compostos Organotiofosforados/administração & dosagem
Convulsões/induzido quimicamente
Maturidade Sexual/efeitos dos fármacos
Suínos
Porco Miniatura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chemical Warfare Agents); 0 (Organothiophosphorus Compounds); 9A4381183B (VX)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:171201
[Lr] Data última revisão:
171201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


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[PMID]:28966132
[Au] Autor:Choi MK; Le MT; Cho H; Yum J; Kang M; Song H; Kim JH; Chung HJ; Hong K; Park C
[Ad] Endereço:Department of Stem Cell and Regenerative Biotechnology, Konkuk University, Seoul, South Korea.
[Ti] Título:Determination of complete sequence information of the human ABO blood group orthologous gene in pigs and breed difference in blood type frequencies.
[So] Source:Gene;640:1-5, 2018 Jan 15.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The sequence information of the genomic form of the human ABO blood group orthologous gene (erythrocyte antigen A, EAA) is not complete in pigs. Therefore, we cloned and characterized the nucleotide sequence of EAA intron 7, which is critical to understand genetic difference between A and 0 blood groups in pigs, covering complete genomic sequence information of EAA excluding a ~560bp unsequencible gap. We also analyzed genetic polymorphisms within EAA intron 7 and exon 8. We found difference in A0 blood group frequencies among pig breeds. In addition, we designed a new genomic DNA-based A0 blood group typing method and improved the accuracy and simplicity of the typing.
[Mh] Termos MeSH primário: Sistema do Grupo Sanguíneo ABO/genética
Cruzamento
Polimorfismo Genético
Análise de Sequência de DNA
[Mh] Termos MeSH secundário: Animais
Frequência do Gene
Genótipo
Seres Humanos
Fenótipo
Suínos
Porco Miniatura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (ABO Blood-Group System)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171003
[St] Status:MEDLINE


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[PMID]:29045496
[Au] Autor:Revel A; Jarzaguet M; Peyron MA; Papet I; Hafnaoui N; Migné C; Mosoni L; Polakof S; Savary-Auzeloux I; Rémond D; Dardevet D
[Ad] Endereço:Université Clermont Auvergne, INRA, UNH, Unité de Nutrition Humaine, PFEM, MetaboHUB-Clermont, CRNH Auvergne, Clermont-Ferrand, France.
[Ti] Título:At same leucine intake, a whey/plant protein blend is not as effective as whey to initiate a transient post prandial muscle anabolic response during a catabolic state in mini pigs.
[So] Source:PLoS One;12(10):e0186204, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Muscle atrophy has been explained by an anabolic resistance following food intake and an increase of dietary protein intake is recommended. To be optimal, a dietary protein has to be effective not only to initiate but also to prolong a muscle anabolic response in a catabolic state. To our knowledge, whether or not a dairy or a dairy/plant protein blend fulfills these criterions is unknown in a muscle wasting situation. OBJECTIVE: Our aim was, in a control and a catabolic state, to measure continuously muscle anabolism in term of intensity and duration in response to a meal containing casein (CAS), whey (WHEY) or a whey/ plant protein blend (BLEND) and to evaluate the best protein source to elicit the best post prandial anabolism according to the physio-pathological state. METHODS: Adult male Yucatan mini pigs were infused with U-13C-Phenylalanine and fed either CAS, WHEY or BLEND. A catabolic state was induced by a glucocorticoid treatment for 8 days (DEX). Muscle protein synthesis, proteolysis and balance were measured with the hind limb arterio-venous differences technique. Repeated time variance analysis were used to assess significant differences. RESULTS: In a catabolic situation, whey proteins were able to initiate muscle anabolism which remained transient in contrast to the stimulated muscle protein accretion with WHEY, CAS or BLEND in healthy conditions. Despite the same leucine intake compared to WHEY, BLEND did not restore a positive protein balance in DEX animals. CONCLUSIONS: Even with WHEY, the duration of the anabolic response was not optimal and has to be improved in a catabolic state. The use of BLEND remained of lower efficiency even at same leucine intake than whey.
[Mh] Termos MeSH primário: Anabolizantes/administração & dosagem
Caseínas/administração & dosagem
Leucina/metabolismo
Atrofia Muscular/dietoterapia
Proteínas de Vegetais Comestíveis/administração & dosagem
[Mh] Termos MeSH secundário: Animais
Ingestão de Alimentos
Glucocorticoides/administração & dosagem
Metabolismo/efeitos dos fármacos
Músculo Esquelético/efeitos dos fármacos
Músculo Esquelético/metabolismo
Atrofia Muscular/metabolismo
Atrofia Muscular/patologia
Período Pós-Prandial/efeitos dos fármacos
Suínos
Porco Miniatura
Soro do Leite/administração & dosagem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anabolic Agents); 0 (Caseins); 0 (Glucocorticoids); 0 (Vegetable Proteins); GMW67QNF9C (Leucine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171019
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186204


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[PMID]:29016656
[Au] Autor:Schuldenzucker V; Schubert R; Muratori LM; Freisfeld F; Rieke L; Matheis T; Schramke S; Motlik J; Kemper N; Radespiel U; Reilmann R
[Ad] Endereço:George-Huntington-Institute, Technology-Park, Muenster, Germany.
[Ti] Título:Behavioral testing of minipigs transgenic for the Huntington gene-A three-year observational study.
[So] Source:PLoS One;12(10):e0185970, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Large animal models of Huntington's disease (HD) may increase the reliability of translating preclinical findings to humans. Long live expectancy offers opportunities particularly for disease modifying approaches, but also challenges. The transgenic (tg) HD minipig model assessed in this study exhibits a high genetic homology with humans, similar body weight, and comparable brain structures. To test long-term safety, tolerability, and efficacy of novel therapeutic approaches in this model reliable assessments applicable longitudinally for several years are warranted for all phenotypical domains relevant in HD. OBJECTIVE: To investigate whether the tests proposed assessing motor, cognitive and behavioral domains can be applied repetitively over a 3-year period in minipigs with acceptable variability or learning effects and whether tgHD minipigs reveal changes in these domains compared to wildtype (wt) minipigs suggesting the development of an HD phenotype. METHODS: A cohort of 14 tgHD and 18 wt minipigs was followed for three years. Tests applied every six months included a tongue coordination and hurdle test for the motor domain, a color discrimination test for cognition, and a dominance test for assessing behavior. Statistical analyses were performed using repeated ANOVA for longitudinal group comparisons and Wilcoxon-tests for intra-visit differences between tgHD and wt minipigs. RESULTS: All tests applied demonstrated feasibility, acceptable variance and good consistency during the three-year period. No significant differences between tgHD and wt minipigs were detected suggesting lack of a phenotype before the age of four years. CONCLUSIONS: The assessment battery presented offers measures in all domains relevant for HD and can be applied in long-term phenotyping studies with tgHD minipigs. The observation of this cohort should be continued to explore the timeline of phenotype development and provide information for future interventional studies.
[Mh] Termos MeSH primário: Comportamento Animal/fisiologia
Doença de Huntington/fisiopatologia
Porco Miniatura/fisiologia
Suínos/fisiologia
[Mh] Termos MeSH secundário: Animais
Animais Geneticamente Modificados
Feminino
Seres Humanos
Proteína Huntingtina/genética
Proteína Huntingtina/fisiologia
Aprendizagem/fisiologia
Língua/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Huntingtin Protein)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171011
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185970


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[PMID]:28961259
[Au] Autor:Li XD; Yang YJ; Wang LY; Qiao SB; Lu XF; Wu YJ; Xu B; Li HF; Gu DF
[Ad] Endereço:Department of Cardiology, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
[Ti] Título:Elevated plasma miRNA-122, -140-3p, -720, -2861, and -3149 during early period of acute coronary syndrome are derived from peripheral blood mononuclear cells.
[So] Source:PLoS One;12(9):e0184256, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: Our previous study has found that circulating microRNA (miRNA, or miR) -122, -140-3p, -720, -2861, and -3149 are significantly elevated during early stage of acute coronary syndrome (ACS). This study was conducted to determine the origin of these elevated plasma miRNAs in ACS. METHODS: qRT-PCR was performed to detect the expression profiles of these 5 miRNAs in liver, spleen, lung, kidney, brain, skeletal muscles, and heart. To determine their origins, these miRNAs were detected in myocardium of acute myocardial infarction (AMI), and as well in platelets and peripheral blood mononuclear cells (PBMCs, including monocytes, circulating endothelial cells (CECs) and lymphocytes) of the AMI pigs and ACS patients. RESULTS: MiR-122 was specifically expressed in liver, and miR-140-3p, -720, -2861, and -3149 were highly expressed in heart. Compared with the sham pigs, miR-122 was highly expressed in the border zone of the ischemic myocardium in the AMI pigs without ventricular fibrillation (P < 0.01), miR-122 and -720 were decreased in platelets of the AMI pigs, and miR-122, -140-3p, -720, -2861, and -3149 were increased in PBMCs of the AMI pigs (all P < 0.05). Compared with the non-ACS patients, platelets miR-720 was decreased and PBMCs miR-122, -140-3p, -720, -2861, and -3149 were increased in the ACS patients (all P < 0.01). Furthermore, PBMCs miR-122, -720, and -3149 were increased in the AMI patients compared with the unstable angina (UA) patients (all P < 0.05). Further origin identification revealed that the expression levels of miR-122 in CECs and lymphocytes, miR-140-3p and -2861 in monocytes and CECs, miR-720 in monocytes, and miR-3149 in CECs were greatly up-regulated in the ACS patients compared with the non-ACS patients, and were higher as well in the AMI patients than that in the UA patients except for the miR-122 in CECs (all P < 0.05). CONCLUSION: The elevated plasma miR-122, -140-3p, -720, -2861, and -3149 in the ACS patients were mainly originated from CECs and monocytes.
[Mh] Termos MeSH primário: Síndrome Coronariana Aguda/sangue
Leucócitos Mononucleares/metabolismo
MicroRNAs/sangue
[Mh] Termos MeSH secundário: Síndrome Coronariana Aguda/genética
Animais
Perfilação da Expressão Gênica
Seres Humanos
Masculino
Reação em Cadeia da Polimerase em Tempo Real
Suínos
Porco Miniatura
Distribuição Tecidual
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (MicroRNAs)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170930
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184256



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