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  1 / 1701 MEDLINE  
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[PMID]:29236723
[Au] Autor:Ververs C; van Zijll Langhout M; Hostens M; Otto M; Govaere J; Durrant B; Van Soom A
[Ad] Endereço:Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan, 9820 Merelbeke, Belgium.
[Ti] Título:Reproductive performance parameters in a large population of game-ranched white rhinoceroses (Ceratotherium simum simum).
[So] Source:PLoS One;12(12):e0187751, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The population of free-roaming white rhinoceroses (Ceratotherium simum) is under serious threat. Captive breeding of this species is therefore becoming more important, but this is challenging and often not successful. Obtaining reproductive reference values is a crucial aspect of improving these breeding results. In this study performed between 2008 and 2016, reproductive performance was analysed in 1,354 animals kept in a 8000 hectares game-ranched environment. Descriptive statistics of this captive population showed an average annual herd growth (%) of 7 .0±0.1 (min -9 -max 15). Average calving rates were calculated as an annual calving rate of 20% and biennial calving rate of 37% adult females calving per year. Females had a median age of 83.2 months at first calving (IQR 72.9-110.7) and inter-calving intervals of 29.2 (IQR 24.6-34.8) months. Furthermore, translocations of animals did not interfere with reproductive success in terms of inter-calving periods or age at first calving. Multivariate models showed a clear seasonal calving pattern with a significant increase of the number of calvings during December-April when compared to April-December. Our results did not show any significant skewed progeny sex ratios. Weather observations showed no significant influence of rain or season on sex ratios of the calves.
[Mh] Termos MeSH primário: Perissodáctilos/fisiologia
[Mh] Termos MeSH secundário: Animais
Feminino
Masculino
Perissodáctilos/crescimento & desenvolvimento
Reprodução
Estações do Ano
Razão de Masculinidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180104
[Lr] Data última revisão:
180104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0187751


  2 / 1701 MEDLINE  
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[PMID]:28686714
[Au] Autor:Michel AL; Lane EP; de Klerk-Lorist LM; Hofmeyr M; van der Heijden EMDL; Botha L; van Helden P; Miller M; Buss P
[Ad] Endereço:Department of Veterinary Tropical Diseases, Bovine Tuberculosis and Brucellosis Research Programme, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa.
[Ti] Título:Experimental Mycobacterium bovis infection in three white rhinoceroses (Ceratotherium simum): Susceptibility, clinical and anatomical pathology.
[So] Source:PLoS One;12(7):e0179943, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Tuberculosis caused by Mycobacterium bovis is endemic in the African buffalo (Syncerus caffer) population in the Kruger National Park and other conservation areas in South Africa. The disease has been diagnosed in a total of 21 free ranging or semi-free ranging wildlife species in the country with highly variable presentations in terms of clinical signs as well as severity and distribution of tuberculous lesions. Most species are spillover or dead-end hosts without significant role in the epidemiology of the disease. White rhinoceroses (Ceratotherium simum) are translocated from the Kruger National Park in substantial numbers every year and a clear understanding of their risk to manifest overt tuberculosis disease and to serve as source of infection to other species is required. We report the findings of experimental infection of three white rhinoceroses with a moderately low dose of a virulent field isolate of Mycobacterium bovis. None of the animals developed clinical signs or disseminated disease. The susceptibility of the white rhinoceros to bovine tuberculosis was confirmed by successful experimental infection based on the ante mortem isolation of M. bovis from the respiratory tract of one rhinoceros, the presence of acid-fast organisms and necrotizing granulomatous lesions in the tracheobronchial lymph nodes and the detection of M. bovis genetic material by PCR in the lungs of two animals.
[Mh] Termos MeSH primário: Animais Selvagens/microbiologia
Mycobacterium bovis/patogenicidade
Perissodáctilos/microbiologia
Tuberculose Bovina/microbiologia
[Mh] Termos MeSH secundário: Animais
Búfalos/microbiologia
Bovinos
Mycobacterium bovis/isolamento & purificação
Tuberculose Bovina/transmissão
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170708
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0179943


  3 / 1701 MEDLINE  
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[PMID]:28495194
[Au] Autor:Peters M; Osmann C; Wohlsein P; Schares G
[Ad] Endereço:Chemisches und Veterinäruntersuchungsamt Westfalen (CVUA) Westfalen, Zur Taubeneiche 10-12, D-59821 Arnsberg, Germany. Electronic address: martin.peters@cvua-westfalen.de.
[Ti] Título:Neospora caninum abortion in a Malayan tapir (Tapirus indicus).
[So] Source:Vet Parasitol;239:37-41, 2017 May 30.
[Is] ISSN:1873-2550
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A captive 17-year old female Malayan tapir (Tapirus indicus) aborted a fetus with a crown rump length of 19cm in early pregnancy. The fetus showed an early state of mummification. Histologically, a multifocal mononuclear encephalitis, myocarditis and periportal hepatitis was present indicating a possible protozoal cause of abortion. Although immunohistologically, Neospora (N.) caninum antigen could not be demonstrated, N. caninum DNA was detected by Polymerase Chain Reaction (PCR) in brain, heart, liver and lung of the fetus. N. caninum DNA was extracted from the aborted fetus and the microsatellite marker MS10 was amplified by PCR and sequenced. The obtained MS10 microsatellite pattern has not been described in Germany yet. Nevertheless, the MS10 pattern was very similar to those reported for N. caninum isolated from dogs and cattle in Germany. Because of the histological pattern and extent of the lesions, neosporosis was suspected as the cause of fetal death and abortion. This case report describes for the first time transplacental transmission of N. caninum and abortion due to neosporosis in a tapir.
[Mh] Termos MeSH primário: Aborto Animal/parasitologia
Coccidiose/veterinária
Neospora
Perissodáctilos
[Mh] Termos MeSH secundário: Feto Abortado
Animais
Animais de Zoológico
Coccidiose/parasitologia
Coccidiose/patologia
Feminino
Gravidez
Complicações Parasitárias na Gravidez/veterinária
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170513
[St] Status:MEDLINE


  4 / 1701 MEDLINE  
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[PMID]:28441468
[Au] Autor:Plochocki JH; Ruiz S; Rodriguez-Sosa JR; Hall MI
[Ad] Endereço:Department of Anatomy, Midwestern University, Glendale, Arizona, United States of America.
[Ti] Título:Histological study of white rhinoceros integument.
[So] Source:PLoS One;12(4):e0176327, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In this study, we report findings from a microscopic analysis of the white rhinoceros (Ceratotherium simum) integumentary ultrastructure. Skin samples from the cheek, shoulder, flank and rump were taken from a 46-year-old female southern white rhinoceros and examined using H&E and elastic histological stains. The epidermis was thickest in the flank (1.003 mm) followed by the rump, cheek and shoulder. The stratum corneum comprised more than half the epidermal thickness. Numerous melanin granules were found in the basal and spinosum layers. The epidermal-dermal junction was characterized by abundant papillary folds increasing surface contact between integument layers. Most of the dermal thickness consisted of organized collagen bundles with scattered elastic fibers. Collagen fiber bundles were thickest in the flank (210.9 µm) followed by shoulder, rump and cheek. Simple coiled sweat glands were present in the dermis, but hair and sebaceous glands were absent. Together, these data suggest the white rhinoceros has a unique integumentary system among large terrestrial herbivores.
[Mh] Termos MeSH primário: Tegumento Comum/anatomia & histologia
Perissodáctilos/anatomia & histologia
Pele/anatomia & histologia
Glândulas Sudoríparas/anatomia & histologia
[Mh] Termos MeSH secundário: Animais
Colágeno/ultraestrutura
Epiderme/anatomia & histologia
Epiderme/ultraestrutura
Feminino
Pele/ultraestrutura
Glândulas Sudoríparas/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9007-34-5 (Collagen)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170426
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0176327


  5 / 1701 MEDLINE  
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[PMID]:28341928
[Au] Autor:Apanaskevich DA; Bermúdez SE
[Ad] Endereço:United States National Tick Collection, The James H. Oliver, Jr. Institute for Coastal Plain Science, Georgia Southern University, Statesboro, Georgia, 30460-8056, USA. dapanaskevich@georgiasouthern.edu.
[Ti] Título:Description of a new species of Ixodes Latreille, 1795 (Acari: Ixodidae) and redescription of I. lasallei Méndez & Ortiz, 1958, parasites of agoutis and pacas (Rodentia: Dasyproctidae, Cuniculidae) in Central and South America.
[So] Source:Syst Parasitol;94(4):463-475, 2017 May.
[Is] ISSN:1573-5192
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Ixodes bocatorensis n. sp. (Acari: Ixodidae), is described based on adults ex agoutis (Rodentia: Dasyproctidae), pacas (Rodentia: Cuniculidae) and "tapir and sloth" (Perissodactyla: Tapiridae and Pilosa) from Colombia, Panama and Venezuela. Adults of I. bocatorensis n. sp. are similar to those of I. lasallei Méndez & Ortiz, 1958 but can be distinguished by the scutum dimensions, punctation pattern, gnathosoma and palpi measurements and their ratios, basis capituli anterior angle and shape of the spur of palpal segment I ventrally. For comparative purposes the female of I. lasallei is redescribed and the true male of this species is described for the first time. Studied adults of I. lasallei were found on agoutis, pacas and ocelot (Carnivora: Felidae) in Colombia, Peru and Venezuela.
[Mh] Termos MeSH primário: Cuniculidae/parasitologia
Ixodes/anatomia & histologia
Ixodes/classificação
[Mh] Termos MeSH secundário: Animais
América Central
Felidae/parasitologia
Feminino
Especificidade de Hospedeiro
Masculino
Perissodáctilos/parasitologia
América do Sul
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171101
[Lr] Data última revisão:
171101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170326
[St] Status:MEDLINE
[do] DOI:10.1007/s11230-017-9718-4


  6 / 1701 MEDLINE  
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[PMID]:28281770
[Au] Autor:De Lange SS; Fuller A; Haw A; Hofmeyr M; Buss P; Miller M; Meyer LC
[Ti] Título:Tremors in white rhinoceroses (Ceratotherium simum) during etorphine-azaperone immobilisation.
[So] Source:J S Afr Vet Assoc;88(0):e1-e10, 2017 Feb 24.
[Is] ISSN:2224-9435
[Cp] País de publicação:South Africa
[La] Idioma:eng
[Ab] Resumo:Little is known about the mechanisms causing tremors during immobilisation of rhinoceros and whether cardiorespiratory supportive interventions alter their intensity. Therefore, we set out to determine the possible mechanisms that lead to muscle tremors and ascertain whether cardiorespiratory supportive interventions affect tremor intensity. We studied tremors and physiological responses during etorphine-azaperone immobilisation in eight boma-held and 14 free-living white rhinoceroses. Repeated measures analysis of variance and a Friedman test were used to determine differences in variables over time and between interventions. Spearman and Pearson correlations were used to test for associations between variables. Tremor intensity measured objectively by activity loggers correlated well (p < 0.0001; r2 = 0.9) with visual observations. Tremor intensity was greatest when animals were severely hypoxaemic and acidaemic. Tremor intensity correlated strongly and negatively with partial pressure of oxygen (PaO2 ) (p = 0.0003; r2 = 0.9995) and potential of hydrogen (pH) (p = 0.02, r2 = 0.97). It correlated strongly and positively with adrenaline concentrations (p = 0.003; r2 = 0.96), and adrenaline correlated strongly and negatively with PaO2 (p = 0.03; r2 = 0.95) and pH (p = 0.03; r2 = 0.94). Therefore, hypoxaemia and acidaemia were likely associated with the intensity of tremors through their activation of the release of tremorgenic levels of adrenaline. Tremors can be reduced if circulating adrenaline is reduced, and this can be achieved by the administration of butorphanol plus oxygen insufflation. Furthermore, to assist with reducing the risks associated with rhinoceros immobilisation, tremor intensity could be used as a clinical indicator of respiratory and metabolic compromise.
[Mh] Termos MeSH primário: Azaperona/efeitos adversos
Etorfina/efeitos adversos
Hipnóticos e Sedativos/efeitos adversos
Hipóxia/veterinária
Perissodáctilos
Tremor/veterinária
[Mh] Termos MeSH secundário: Análise de Variância
Animais
Butorfanol/uso terapêutico
Epinefrina/sangue
Hipóxia/induzido quimicamente
Hipóxia/fisiopatologia
Imobilização/métodos
Imobilização/veterinária
Masculino
Monitorização Fisiológica
Antagonistas de Entorpecentes/uso terapêutico
Perissodáctilos/sangue
Perissodáctilos/fisiologia
Distribuição Aleatória
África do Sul
Tremor/induzido quimicamente
Tremor/tratamento farmacológico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hypnotics and Sedatives); 0 (Narcotic Antagonists); 19BV78AK7W (Azaperone); 42M2Y6NU9O (Etorphine); QV897JC36D (Butorphanol); YKH834O4BH (Epinephrine)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170912
[Lr] Data última revisão:
170912
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170311
[St] Status:MEDLINE
[do] DOI:10.4102/jsava.v88i0.1466


  7 / 1701 MEDLINE  
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[PMID]:28273061
[Au] Autor:Weyrich LS; Duchene S; Soubrier J; Arriola L; Llamas B; Breen J; Morris AG; Alt KW; Caramelli D; Dresely V; Farrell M; Farrer AG; Francken M; Gully N; Haak W; Hardy K; Harvati K; Held P; Holmes EC; Kaidonis J; Lalueza-Fox C; de la Rasilla M; Rosas A; Semal P; Soltysiak A; Townsend G; Usai D; Wahl J; Huson DH; Dobney K; Cooper A
[Ad] Endereço:Australian Centre for Ancient DNA, School of Biological Sciences and The Environment Institute, University of Adelaide, Adelaide, South Australia, Australia.
[Ti] Título:Neanderthal behaviour, diet, and disease inferred from ancient DNA in dental calculus.
[So] Source:Nature;544(7650):357-361, 2017 04 20.
[Is] ISSN:1476-4687
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Recent genomic data have revealed multiple interactions between Neanderthals and modern humans, but there is currently little genetic evidence regarding Neanderthal behaviour, diet, or disease. Here we describe the shotgun-sequencing of ancient DNA from five specimens of Neanderthal calcified dental plaque (calculus) and the characterization of regional differences in Neanderthal ecology. At Spy cave, Belgium, Neanderthal diet was heavily meat based and included woolly rhinoceros and wild sheep (mouflon), characteristic of a steppe environment. In contrast, no meat was detected in the diet of Neanderthals from El Sidrón cave, Spain, and dietary components of mushrooms, pine nuts, and moss reflected forest gathering. Differences in diet were also linked to an overall shift in the oral bacterial community (microbiota) and suggested that meat consumption contributed to substantial variation within Neanderthal microbiota. Evidence for self-medication was detected in an El Sidrón Neanderthal with a dental abscess and a chronic gastrointestinal pathogen (Enterocytozoon bieneusi). Metagenomic data from this individual also contained a nearly complete genome of the archaeal commensal Methanobrevibacter oralis (10.2× depth of coverage)-the oldest draft microbial genome generated to date, at around 48,000 years old. DNA preserved within dental calculus represents a notable source of information about the behaviour and health of ancient hominin specimens, as well as a unique system that is useful for the study of long-term microbial evolution.
[Mh] Termos MeSH primário: DNA Antigo/análise
Cálculos Dentários/química
Dieta/história
Preferências Alimentares
Saúde/história
Homem de Neandertal/microbiologia
Homem de Neandertal/psicologia
[Mh] Termos MeSH secundário: Animais
Bélgica
Carnivoridade
Cavernas
Enterocytozoon/genética
Enterocytozoon/isolamento & purificação
Genoma Bacteriano/genética
História Antiga
Seres Humanos
Intestinos/microbiologia
Carne/história
Methanobrevibacter/genética
Methanobrevibacter/isolamento & purificação
Boca/microbiologia
Pan troglodytes/microbiologia
Penicillium/química
Perissodáctilos
Ovinos
Espanha
Estômago/microbiologia
Simbiose
Fatores de Tempo
Vegetarianos/história
[Pt] Tipo de publicação:HISTORICAL ARTICLE; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Ancient)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170706
[Lr] Data última revisão:
170706
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170309
[St] Status:MEDLINE
[do] DOI:10.1038/nature21674


  8 / 1701 MEDLINE  
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[PMID]:28210960
[Au] Autor:Apanaskevich DA; Domínguez LG; Torres SS; Bernal JA; Montenegro VM; Bermúdez SE
[Ad] Endereço:United States National Tick Collection, James H. Oliver, Jr. Institute for Coastal Plain Science, Georgia Southern University, Statesboro, GA, 30460-8056, USA.
[Ti] Título:First description of the male and redescription of the female of Ixodes tapirus Kohls, 1956 (Acari: Ixodidae), a parasite of tapirs (Perissodactyla: Tapiridae) from the mountains of Colombia, Costa Rica and Panama.
[So] Source:Syst Parasitol;94(3):413-422, 2017 Mar.
[Is] ISSN:1573-5192
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The male of Ixodes tapirus Kohls, 1956 (Acari: Ixodidae) is described for the first time and the female is redescribed in greater detail. Adults of I. tapirus are similar to those of Ixodes guatemalensis Kohls, 1956, Ixodes lasallei Méndez & Ortiz, 1958, Ixodes montoyanus Cooley, 1944 and Ixodes venezuelensis Kohls, 1953 but can be distinguished by their overall size, the amount of sclerotisation of the conscutum and accessory plates, the shape of the scutum, the number of punctations and their pattern on the conscutum and scutum, the depth of the punctations on the basis capituli dorsally, the shape and size of the porose areas and the size and shape of the auriculae. Adults of I. tapirus were collected from tapirs and vegetation in the mountains of Colombia, Panama and recorded from Costa Rica for the first time.
[Mh] Termos MeSH primário: Ixodes/classificação
Perissodáctilos/parasitologia
[Mh] Termos MeSH secundário: Animais
Colômbia
Costa Rica
Feminino
Ixodes/anatomia & histologia
Masculino
Panamá
Plantas/parasitologia
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171101
[Lr] Data última revisão:
171101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170218
[St] Status:MEDLINE
[do] DOI:10.1007/s11230-017-9706-8


  9 / 1701 MEDLINE  
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[PMID]:28155285
[Au] Autor:Horak IG; Boshoff CR; Cooper DV; Foggin CM; Govender D; Harrison A; Hausler G; Hofmeyr M; Kilian JW; MacFadyen DN; Nel PJ; Peinke D; Squarre D; Zimmermann D
[Ad] Endereço:Department of Veterinary Tropical Diseases, University of Pretoria. ivan.horak@up.ac.za.
[Ti] Título:Parasites of domestic and wild animals in South Africa. XLIX. Ticks (Acari: Ixodidae) infesting white and black rhinoceroses in southern Africa.
[So] Source:Onderstepoort J Vet Res;84(1):e1-e11, 2017 Jan 30.
[Is] ISSN:2219-0635
[Cp] País de publicação:South Africa
[La] Idioma:eng
[Ab] Resumo:The objectives of the study were to determine the species composition of ticks infesting white and black rhinoceroses in southern Africa as well as the conservation status of those tick species that prefer rhinos as hosts. Ticks were collected opportunistically from rhinos that had been immobilised for management purposes, and 447 white rhinoceroses (Ceratotherium simum) and 164 black rhinoceroses (Diceros bicornis) were sampled in South Africa, 61 black rhinos in Namibia, 18 white and 12 black rhinos in Zimbabwe, and 24 black rhinos in Zambia. Nineteen tick species were recovered, of which two species, Amblyomma rhinocerotis and Dermacentor rhinocerinus, prefer rhinos as hosts. A. rhinocerotis was collected only in the northeastern KwaZulu-Natal reserves of South Africa and is endangered, while D. rhinocerinus is present in these reserves as well as in the Kruger National Park and surrounding conservancies. Eight of the tick species collected from the rhinos are ornate, and seven species are regularly collected from cattle. The species present on rhinos in the eastern, moister reserves of South Africa were amongst others Amblyomma hebraeum, A. rhinocerotis, D. rhinocerinus, Rhipicephalus maculatus, Rhipicephalus simus and Rhipicephalus zumpti, while those on rhinos in the Karoo and the drier western regions, including Namibia, were the drought-tolerant species, Hyalomma glabrum, Hyalomma rufipes, Hyalomma truncatum and Rhipicephalus gertrudae. The species composition of ticks on rhinoceroses in Zambia differed markedly from those of the other southern African countries in that Amblyomma sparsum, Amblyomma tholloni and Amblyomma variegatum accounted for the majority of infestations.
[Mh] Termos MeSH primário: Ixodidae/fisiologia
Perissodáctilos/parasitologia
Infestações por Carrapato/veterinária
[Mh] Termos MeSH secundário: Animais
Animais Selvagens/parasitologia
Dermacentor/fisiologia
Feminino
Gado/parasitologia
Masculino
Namíbia/epidemiologia
Rhipicephalus/fisiologia
África do Sul/epidemiologia
Especificidade da Espécie
Infestações por Carrapato/epidemiologia
Zâmbia/epidemiologia
Zimbábue/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170223
[Lr] Data última revisão:
170223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170204
[St] Status:MEDLINE
[do] DOI:10.4102/ojvr.v84i1.1301


  10 / 1701 MEDLINE  
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[PMID]:28152184
[Au] Autor:Hooijberg EH; Steenkamp G; du Preez JP; Goddard A
[Ad] Endereço:Department of Companion Animal Clinical Studies, Faculty of Veterinary Science, University of Pretoria, Pretoria, South Africa.
[Ti] Título:Analytic and quality control validation and assessment of field performance of a point-of-care chemistry analyzer for use in the White rhinoceros.
[So] Source:Vet Clin Pathol;46(1):100-110, 2017 Mar.
[Is] ISSN:1939-165X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: A chemistry point-of-care analyzer would be useful for evaluating injured wildlife, particularly White rhinoceros (Ceratotherium simum) that survive poaching attempts. The IDEXX VetTest could be suitable, but species-specific validation, development of a statistical quality control (QC) strategy, and evaluation under field conditions are necessary. OBJECTIVES: The objectives were to (1) validate the VetTest for the White rhinoceros, (2) perform QC validation on the VetTest and generate a statistical QC strategy, and (3) apply this QC strategy to monitor performance under typical field conditions. METHODS: Differences between White rhinoceros heparin plasma and serum, short-term imprecision, and reportable range using rhinoceros plasma and long-term imprecision using commercial quality control material (QCM) were assessed against prescribed total allowable error (TE ) for up to 15 analytes. Quality control validation was performed using data from the long-term imprecision study and TE . A QC strategy using QCM was developed and used to monitor performance under field conditions. RESULTS: Imprecision was acceptable for all analytes except for ALP, ALT, and AST at low activities. The reportable range for AST and LDH differed from the manufacturer's specifications. Eleven analytes were suitable for statistical QC using the 1 rule, 3 using the 2 rule; ALP was not suitable. In the field, observed error was < TE for all 15 analytes and the sigma metric was > 3.0 for 12 analytes. CONCLUSIONS: The VetTest is suitable for use in the White rhinoceros. Statistical QC is possible for most analytes and useful for evaluation of field performance.
[Mh] Termos MeSH primário: Análise Química do Sangue/veterinária
Erros de Diagnóstico/veterinária
Perissodáctilos/sangue
Sistemas Automatizados de Assistência Junto ao Leito/normas
[Mh] Termos MeSH secundário: Animais
Análise Química do Sangue/instrumentação
Análise Química do Sangue/normas
Controle de Qualidade
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170203
[St] Status:MEDLINE
[do] DOI:10.1111/vcp.12456



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