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Pesquisa : B01.050.150.900.649.313.988.400.600.037 [Categoria DeCS]
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[PMID]:28466296
[Au] Autor:Suryawanashi YR; Zhang T; Woyczesczyk HM; Christie J; Byers E; Kohler S; Eversole R; Mackenzie C; Essani K
[Ad] Endereço:Laboratory of Virology, Department of Biological Sciences, Western Michigan University, Kalamazoo, MI, 49008-5410, USA.
[Ti] Título:T-independent response mediated by oncolytic tanapoxvirus recombinants expressing interleukin-2 and monocyte chemoattractant protein-1 suppresses human triple negative breast tumors.
[So] Source:Med Oncol;34(6):112, 2017 Jun.
[Is] ISSN:1559-131X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Human triple negative breast cancer (TNBC) is an aggressive disease, associated with a high rate of recurrence and metastasis. Current therapeutics for TNBC are limited, highly toxic and show inconsistent efficacy due to a high degree of intra-tumoral and inter-tumoral heterogeneity. Oncolytic viruses (OVs) are an emerging treatment option for cancers. Several OVs are currently under investigation in preclinical and clinical settings. Here, we examine the oncolytic potential of two tanapoxvirus (TPV) recombinants expressing mouse monocyte chemoattractant protein (mMCP)-1 [also known as mCCL2] and mouse interleukin (mIL)-2, in human TNBC, in vitro and in vivo. Both wild-type (wt) TPV and TPV recombinants demonstrated efficient replicability in human TNBC cells and killed cancer cell efficiently in a dose-dependent manner in vitro. TPV/∆66R/mCCL2 and TPV/∆66R/mIL-2 expressing mCCL2 and mIL-2, respectively, suppressed the growth of MDA-MB-231 tumor xenografts in nude mice significantly, as compared to the mock-injected tumors. Histological analysis of tumors showed areas of viable tumor cells, necrotic foci and immune cell accumulation in virus-treated tumors. Moreover, TPV/∆66R/mIL-2-treated tumors showed a deep infiltration of mononuclear immune cells into the tumor capsule and focal cell death in tumors. In conclusion, TPV recombinants expressing mCCL2 and mIL-2 showed a significant therapeutic effect in MDA-MB-231 tumor xenografts, in nude mice through induction of potent antitumor immune responses. Considering the oncolytic potency of armed oncolytic TPV recombinants expressing mCCL2 and mIL-2 in an experimental nude mouse model, these viruses merit further investigation as alternative treatment options for human breast cancer.
[Mh] Termos MeSH primário: Quimiocina CCL2/metabolismo
Imunoterapia/métodos
Interleucina-2/metabolismo
Vírus Oncolíticos/genética
Neoplasias de Mama Triplo Negativas/metabolismo
Yatapoxvirus/genética
[Mh] Termos MeSH secundário: Animais
Aotidae
Linhagem Celular
Quimiocina CCL2/genética
Quimiocina CCL2/imunologia
Seres Humanos
Interleucina-2/genética
Interleucina-2/imunologia
Masculino
Camundongos
Camundongos Nus
Vírus Oncolíticos/metabolismo
Neoplasias de Mama Triplo Negativas/genética
Neoplasias de Mama Triplo Negativas/imunologia
Ensaios Antitumorais Modelo de Xenoenxerto
Yatapoxvirus/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chemokine CCL2); 0 (Interleukin-2)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180111
[Lr] Data última revisão:
180111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1007/s12032-017-0973-7


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[PMID]:28972927
[Au] Autor:Nahabedian J; Sharma A; Kaczmarek ME; Wilkerson GK; Sawyer SL; Overbaugh J
[Ad] Endereço:Human Biology Division, Fred Hutchinson Cancer Research Center, Seattle, WA, United States; Pathobiology, University of Washington, Seattle, WA, United States.
[Ti] Título:Owl monkey CCR5 reveals synergism between CD4 and CCR5 in HIV-1 entry.
[So] Source:Virology;512:180-186, 2017 Dec.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Studying HIV-1 replication in the presence of functionally related proteins from different species has helped define host determinants of HIV-1 infection. Humans and owl monkeys, but not macaques, encode a CD4 receptor that permits entry of transmissible HIV-1 variants due to a single residue difference. However, little is known about whether divergent CCR5 receptor proteins act as determinants of host-range. Here we show that both owl monkey (Aotus vociferans) CD4 and CCR5 receptors are functional for the entry of transmitted HIV-1 when paired with human versions of the other receptor. By contrast, the owl monkey CD4/CCR5 pair is generally a suboptimal receptor combination, although there is virus-specific variation in infection with owl monkey receptors. Introduction of the human residues 15Y and 16T within a sulfation motif into owl monkey CCR5 resulted in a gain of function. These findings suggest there is cross-talk between CD4 and CCR5 involving the sulfation motif.
[Mh] Termos MeSH primário: Antígenos CD4/fisiologia
HIV-1/fisiologia
Receptores CCR5/metabolismo
Internalização do Vírus
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Aotidae
Células HEK293
Seres Humanos
Mutação
Receptores CCR5/genética
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (CD4 Antigens); 0 (Receptors, CCR5)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171004
[St] Status:MEDLINE


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[PMID]:28782517
[Au] Autor:Suárez CF; Pabón L; Barrera A; Aza-Conde J; Patarroyo MA; Patarroyo ME
[Ad] Endereço:Fundación Instituto de Inmunología de Colombia (FIDIC), Bogotá D.C., Colombia; Universidad del Rosario, Bogotá D.C., Colombia; Universidad de Ciencias Aplicadas y Ambientales (UDCA), Bogotá, Colombia.
[Ti] Título:Structural analysis of owl monkey MHC-DR shows that fully-protective malaria vaccine components can be readily used in humans.
[So] Source:Biochem Biophys Res Commun;491(4):1062-1069, 2017 Sep 30.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:More than 50 years ago the owl monkey (genus Aotus) was found to be highly susceptible to developing human malaria, making it an excellent experimental model for this disease. Microbes and parasites' (especially malaria) tremendous genetic variability became resolved during our malaria vaccine development, involving conserved peptides having high host cell binding activity (cHABPs); however, cHABPs are immunologically silent and must be specially modified (mHABPs) to induce a perfect fit into major histocompatibility complex (MHC) molecules (HLA in humans). Since malarial immunity is mainly antibody-mediated and controlled by the HLA-DRB genetic region, ∼1000 Aotus have been molecularly characterised for MHC-DRB, revealing striking similarity between human and Aotus MHC-DRB repertories. Such convergence suggested that a large group of immune protection-inducing protein structures (IMPIPS), highly immunogenic and protection inducers against malarial intravenous challenge in Aotus, could easily be used in humans for inducing full protection against malaria. We highlight the value of a logical and rational methodology for developing a vaccine in an appropriate animal model: Aotus monkeys.
[Mh] Termos MeSH primário: Antígenos de Histocompatibilidade Classe II/química
Antígenos de Histocompatibilidade Classe II/imunologia
Vacinas Antimaláricas/química
Vacinas Antimaláricas/imunologia
[Mh] Termos MeSH secundário: Animais
Reações Antígeno-Anticorpo
Aotidae
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Histocompatibility Antigens Class II); 0 (Malaria Vaccines)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170808
[St] Status:MEDLINE


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[PMID]:28549586
[Au] Autor:Reyes C; Rojas-Luna R; Aza-Conde J; Tabares L; Patarroyo MA; Patarroyo ME
[Ad] Endereço:Fundación Instituto de Inmunología de Colombia (FIDIC), Bogotá, Colombia; Universidad Nacional de Colombia, Bogotá, Colombia.
[Ti] Título:Critical role of HLA-DRß* binding peptides' peripheral flanking residues in fully-protective malaria vaccine development.
[So] Source:Biochem Biophys Res Commun;489(3):339-345, 2017 Jul 29.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A vaccine candidate component must fit perfectly into the antigen presenting HLA-DRß* molecule's groove (or canonical nonapeptide) peptide binding region (PBR) during antigen presentation to the T-cell receptor (TCR), conforming a specific and stable macromolecular complex and induce an appropriate immune response. Antigen's peripheral flanking residues (PFR, positions (p) -p2 and p10) must thus establish strong interactions with the HLA-DRß* - TCR complex. These amino acids (aa) have specific physico-chemical characteristics enabling differentiation between non-protective but antibody-inducer (NPAI), short-lived protection inducer (SLPI) and long-lasting protection inducer (LLPI) peptides when used as an antimalarial vaccine component. Their identification (through H-NMR and Aotus monkey immunization) and proper modification contributes to a logical and rational methodology for long-lasting and protective immunological memory.
[Mh] Termos MeSH primário: Cadeias HLA-DRB1/química
Cadeias HLA-DRB1/imunologia
Vacinas Antimaláricas/química
Vacinas Antimaláricas/imunologia
Peptídeos/química
Peptídeos/imunologia
[Mh] Termos MeSH secundário: Animais
Aotidae
Sítios de Ligação
Peptídeos/síntese química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (HLA-DRB1 Chains); 0 (Malaria Vaccines); 0 (Peptides)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170528
[St] Status:MEDLINE


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[PMID]:28369492
[Au] Autor:Hirai H; Hirai Y; Morimoto M; Kaneko A; Kamanaka Y; Koga A
[Ad] Endereço:Primate Research Institute, Kyoto University, Inuyama, Aichi, Japan.
[Ti] Título:Night Monkey Hybrids Exhibit De Novo Genomic and Karyotypic Alterations: The First Such Case in Primates.
[So] Source:Genome Biol Evol;9(4):945-955, 2017 Apr 01.
[Is] ISSN:1759-6653
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Using molecular chromosomal analyses, we discovered night monkey hybrids produced in captivity from matings between a female Aotus azarae boliviensis (2n = 50) and a male Aotus lemurinus griseimembra (2n = 53). The parents produced seven offspring in total, including one male and six females-a pattern consistent with Haldane's rule. Chromosomal studies were conducted on four of the hybrid offspring. Two of them showed relatively "simple" mixture karyotypes, including different chromosome numbers (2n = 51, 52), which were formed because of a heteromorphic autosome pair in the father (n = 26, 27). The other two hybrid monkeys exhibited de novo genomic and karyotypic alterations. Detailed analysis of the alterations revealed that one individual carried a mixture karyotype of the two parental species and an X chromosome trisomy (53,XXX). The second individual displayed trisomy of chromosome 18 (52,XX,+18) and a reciprocal translocation between autosomes 21 and 23 (52,XX,+18,t(21;23)). Interestingly, the second monkey exhibited mosaicism among blood cells (mos52,XX,+18[87]/52,XX,+18,t(21;23)[85]), but only a single karyotype (52,XX,+18) in skin fibroblast cells. The X- and 18-trisomies were derived from a doubling of the mother's chromosomes in early embryonic cell division, and the reciprocal translocation likely developed in the bone marrow of the offspring, considering that it was observed only in blood cells. Such occurrence of trisomies in hybrid individuals is a unique finding in placental mammals.
[Mh] Termos MeSH primário: Aotidae/genética
Primatas/genética
Cromossomo X/genética
[Mh] Termos MeSH secundário: Animais
Bandeamento Cromossômico
Cromossomos Humanos X/genética
Feminino
Fibroblastos
Seres Humanos
Hibridização Genética
Cariotipagem
Masculino
Aberrações dos Cromossomos Sexuais
Transtornos do Cromossomo Sexual no Desenvolvimento Sexual/genética
Trissomia/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170505
[Lr] Data última revisão:
170505
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170404
[St] Status:MEDLINE
[do] DOI:10.1093/gbe/evx058


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[PMID]:28222841
[Au] Autor:Nehete PN; Nehete BP; Chitta S; Williams LE; Abee CR
[Ad] Endereço:Department of Veterinary Sciences, MD Anderson Cancer Center, University of Texas, Bastrop, Graduate School of Biomedical Sciences, The University of Texas, Houston, Texas;, Email: pnehete@mdanderson.org.
[Ti] Título:Phenotypic and Functional Characterization of Peripheral Blood Lymphocytes from Various Age- and Sex-Specific Groups of Owl Monkeys ( ).
[So] Source:Comp Med;67(1):67-78, 2017 Feb 01.
[Is] ISSN:1532-0820
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Owl monkeys (Aotus nancymaae) are New World NHP that serve an important role in vaccine development and as a model for human disease conditions such as malaria. Despite the past contributions of this animal model, limited information is available about the phenotype and functional properties of peripheral blood lymphocytes in reference to sex and age. Using a panel of human antibodies and a set of standardized human immune assays, we identified and characterized various peripheral blood lymphocyte subsets, evaluated the immune functions of T cells, and analyzed cytokines relative to sex and age in healthy owl monkeys. We noted age- and sex-dependent changes in CD28+ (an essential T cell costimulatory molecule) and CD95+ (an apoptotic surface marker) T cells and various levels of cytokines in the plasma. In immune assays of freshly isolated peripheral blood mononuclear cells, IFNγ and perforin responses were significantly higher in female than in male monkeys and in young adults than in juvenile and geriatric groups, despite similar lymphocyte (particularly T cell) populations in these groups. Our current findings may be useful in exploring Aotus monkeys as a model system for the study of aging, susceptibility to infectious diseases, and age-associated differences in vaccine efficacy, and other challenges particular to pediatric and geriatric patients.
[Mh] Termos MeSH primário: Fatores Etários
Anticorpos Monoclonais/imunologia
Aotidae/imunologia
Modelos Animais de Doenças
Fatores Sexuais
Linfócitos T/imunologia
[Mh] Termos MeSH secundário: Envelhecimento
Animais
Biomarcadores/sangue
Ensaio de Imunoadsorção Enzimática
ELISPOT
Feminino
Seres Humanos
Interferon gama/imunologia
Masculino
Perforina/imunologia
Fenótipo
Distribuição Aleatória
Linfócitos T/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Monoclonal); 0 (Biomarkers); 126465-35-8 (Perforin); 82115-62-6 (Interferon-gamma)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170223
[St] Status:MEDLINE


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[PMID]:28179404
[Au] Autor:Obaldia N; Stockelman MG; Otero W; Cockrill JA; Ganeshan H; Abot EN; Zhang J; Limbach K; Charoenvit Y; Doolan DL; Tang DC; Richie TL
[Ad] Endereço:Center for the Evaluation of Antimalarial Drugs and Vaccines, Tropical Medicine Research/Gorgas Memorial Institute of Health Studies, Panama City, Panama nobaldia@hsph.harvard.edu trichie@sanaria.com.
[Ti] Título:A Plasmodium vivax Plasmid DNA- and Adenovirus-Vectored Malaria Vaccine Encoding Blood-Stage Antigens AMA1 and MSP1 in a Prime/Boost Heterologous Immunization Regimen Partially Protects Aotus Monkeys against Blood-Stage Challenge.
[So] Source:Clin Vaccine Immunol;24(4), 2017 Apr.
[Is] ISSN:1556-679X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Malaria is caused by parasites of the genus , which are transmitted to humans by the bites of mosquitoes. After the elimination of , it is predicted that will remain an important cause of morbidity and mortality outside Africa, stressing the importance of developing a vaccine against malaria. In this study, we assessed the immunogenicity and protective efficacy of two antigens, apical membrane antigen 1 (AMA1) and the 42-kDa C-terminal fragment of merozoite surface protein 1 (MSP1 ) in a plasmid recombinant DNA prime/adenoviral (Ad) vector boost regimen in monkeys. Groups of 4 to 5 monkeys were immunized with plasmid DNA alone, Ad alone, prime/boost regimens with each antigen, prime/boost regimens with both antigens, and empty vector controls and then subjected to blood-stage challenge. The heterologous immunization regimen with the antigen pair was more protective than either antigen alone or both antigens delivered with a single vaccine platform, on the basis of their ability to induce the longest prepatent period and the longest time to the peak level of parasitemia, the lowest peak and mean levels of parasitemia, the smallest area under the parasitemia curve, and the highest self-cure rate. Overall, prechallenge MSP1 antibody titers strongly correlated with a decreased parasite burden. Nevertheless, a significant proportion of immunized animals developed anemia. In conclusion, the plasmid DNA/Ad serotype 5 vaccine encoding blood-stage parasite antigens AMA1 and MSP1 in a heterologous prime/boost immunization regimen provided significant protection against blood-stage challenge in monkeys, indicating the suitability of these antigens and this regimen for further development.
[Mh] Termos MeSH primário: Antígenos de Protozoários/imunologia
Vacinas Antimaláricas/imunologia
Malária Vivax/prevenção & controle
Proteínas de Membrana/imunologia
Proteína 1 de Superfície de Merozoito/imunologia
Proteínas de Protozoários/imunologia
Vacinas de DNA/imunologia
[Mh] Termos MeSH secundário: Anemia/prevenção & controle
Animais
Anticorpos Antiprotozoários/sangue
Aotidae
Modelos Animais de Doenças
Feminino
Vacinas Antimaláricas/administração & dosagem
Malária Vivax/imunologia
Masculino
Parasitemia/prevenção & controle
Resultado do Tratamento
Vacinas de DNA/administração & dosagem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Protozoan); 0 (Antigens, Protozoan); 0 (Malaria Vaccines); 0 (Membrane Proteins); 0 (Merozoite Surface Protein 1); 0 (Protozoan Proteins); 0 (Vaccines, DNA); 0 (apical membrane antigen I, Plasmodium)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171005
[Lr] Data última revisão:
171005
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170210
[St] Status:MEDLINE


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[PMID]:28095375
[Au] Autor:Svensson MS; Shanee S; Shanee N; Bannister FB; Cervera L; Donati G; Huck M; Jerusalinsky L; Juarez CP; Maldonado AM; Martinez Mollinedo J; Méndez-Carvajal PG; Molina Argandoña MA; Mollo Vino AD; Nekaris KA; Peck M; Rey-Goyeneche J; Spaan D; Nijman V
[Ad] Endereço:Nocturnal Primate Research Group, Oxford Brookes University, Oxford, UK.
[Ti] Título:Disappearing in the Night: An Overview on Trade and Legislation of Night Monkeys in South and Central America.
[So] Source:Folia Primatol (Basel);87(5):332-348, 2016.
[Is] ISSN:1421-9980
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The international trade in night monkeys (Aotus spp.), found throughout Central and South America, has been regulated by the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) since 1975. We present a quantitative analysis of this trade from all 9 range countries, over 4 decades, and compare domestic legislation to CITES regulations. Night monkeys were exported from 8 of the 9 habitat countries, totalling 5,968 live individuals and 7,098 specimens, with trade of live individuals declining over time. In terms of species, the most commonly traded was Aotus nancymaae (present in Brazil, Colombia, Peru) followed by A. vociferans (Brazil, Colombia, Ecuador, Peru) and A. zonalis (Colombia, Panama). There was no significant correlation between levels of trade and species' geographic range size or the number of countries in which a species occurs. Five countries have legislation that meets CITES requirements for implementation, whereas the other 4 countries' legislation showed deficiencies. Research conducted in Colombia, Peru, and Brazil suggests significant cross-border trade not captured in official international trade registers. Although international trade has diminished, current trends suggest that populations of rarer species may be under unsustainable pressure. Further research is needed to quantify real trade numbers occurring between habitat countries.
[Mh] Termos MeSH primário: Aotidae
Comércio/legislação & jurisprudência
Espécies em Perigo de Extinção/legislação & jurisprudência
[Mh] Termos MeSH secundário: Animais
América Central
Conservação dos Recursos Naturais/legislação & jurisprudência
Internacionalidade
América do Sul
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170131
[Lr] Data última revisão:
170131
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170118
[St] Status:MEDLINE
[do] DOI:10.1159/000454803


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[PMID]:27519457
[Au] Autor:Perea-Rodriguez JP; Fernandez-Duque E; Corley M; Spence-Aizenberg A
[Ad] Endereço:Department of Anthropology, Yale University, New Haven, Connecticut. Juan.Perea-Rodriguez@Yale.edu.
[Ti] Título:An international workshop to launch P.A.I.R., a program on Aotus integrated research.
[So] Source:Evol Anthropol;25(4):183, 2016 Jul.
[Is] ISSN:1520-6505
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Antropologia Física/educação
Antropologia Física/organização & administração
Aotidae
Pesquisa/educação
Pesquisa/organização & administração
[Mh] Termos MeSH secundário: Animais
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170124
[Lr] Data última revisão:
170124
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160814
[St] Status:MEDLINE
[do] DOI:10.1002/evan.21499


  10 / 498 MEDLINE  
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[PMID]:27463518
[Au] Autor:Shaw-Saliba K; Thomson-Luque R; Obaldía N; Nuñez M; Dutary S; Lim C; Barnes S; Kocken CH; Duraisingh MT; Adams JH; Pasini EM
[Ad] Endereço:Department of Immunology and Infectious Diseases, Harvard T. H. Chan School of Public Health, Boston, Massachusetts, United States of America.
[Ti] Título:Insights into an Optimization of Plasmodium vivax Sal-1 In Vitro Culture: The Aotus Primate Model.
[So] Source:PLoS Negl Trop Dis;10(7):e0004870, 2016 Jul.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Malaria is one of the most significant tropical diseases, and of the Plasmodium species that cause human malaria, P. vivax is the most geographically widespread. However, P. vivax remains a relatively neglected human parasite since research is typically limited to laboratories with direct access to parasite isolates from endemic field settings or from non-human primate models. This restricted research capacity is in large part due to the lack of a continuous P. vivax in vitro culture system, which has hampered the ability for experimental research needed to gain biological knowledge and develop new therapies. Consequently, efforts to establish a long-term P. vivax culture system are confounded by our poor knowledge of the preferred host cell and essential nutrients needed for in vitro propagation. Reliance on very heterogeneous P. vivax field isolates makes it difficult to benchmark parasite characteristics and further complicates development of a robust and reliable culture method. In an effort to eliminate parasite variability as a complication, we used a well-defined Aotus-adapted P. vivax Sal-1 strain to empirically evaluate different short-term in vitro culture conditions and compare them with previous reported attempts at P. vivax in vitro culture Most importantly, we suggest that reticulocyte enrichment methods affect invasion efficiency and we identify stabilized forms of nutrients that appear beneficial for parasite growth, indicating that P. vivax may be extremely sensitive to waste products. Leuko-depletion methods did not significantly affect parasite development. Formatting changes such as shaking and static cultures did not seem to have a major impact while; in contrast, the starting haematocrit affected both parasite invasion and growth. These results support the continued use of Aotus-adapted Sal-1 for development of P. vivax laboratory methods; however, further experiments are needed to optimize culture conditions to support long-term parasite development.
[Mh] Termos MeSH primário: Malária Vivax/parasitologia
Plasmodium vivax
[Mh] Termos MeSH secundário: Animais
Aotidae
Técnicas de Cultura de Células/métodos
Meios de Cultura
Feminino
Plasmodium vivax/classificação
Reticulócitos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Culture Media)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170614
[Lr] Data última revisão:
170614
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160728
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0004870



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