Base de dados : MEDLINE
Pesquisa : B01.050.150.900.649.313.988.400.600.150.150.114 [Categoria DeCS]
Referências encontradas : 3390 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 339 ir para página                         

  1 / 3390 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29385211
[Au] Autor:Hiwaki O; Miyaguchi H
[Ad] Endereço:Graduate School of Information Sciences, Hiroshima City University, Hiroshima, Japan.
[Ti] Título:Noninvasive measurement of dynamic brain signals using light penetrating the brain.
[So] Source:PLoS One;13(1):e0192095, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Conventional techniques for the noninvasive measurement of brain activity involve critical limitations in spatial or temporal resolution. Here, we propose the method for noninvasive brain function measurement with high spatiotemporal resolution using optical signals. We verified that diffused near-infrared light penetrating through the upper jaw and into the skull, which we term as optoencephalography (OEG), leads to the detection of dynamic brain signals that vary concurrently with the electrophysiological neural activity. We measured the OEG signals following the stimulation of the median nerve in common marmosets. The OEG signal response was tightly coupled with the electrophysiological response represented by the somatosensory evoked potential (SSEP). The OEG measurement is also shown to offer rather clear discrimination of brain signals.
[Mh] Termos MeSH primário: Encéfalo/fisiopatologia
Raios Infravermelhos
[Mh] Termos MeSH secundário: Animais
Callithrix
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180201
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0192095


  2 / 3390 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28989098
[Au] Autor:Garbino GST; Martins-Junior AMG
[Ad] Endereço:PPG-Zoologia, Departamento de Zoologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil. Electronic address: antonio_mgmartins@hotmail.com.
[Ti] Título:Phenotypic evolution in marmoset and tamarin monkeys (Cebidae, Callitrichinae) and a revised genus-level classification.
[So] Source:Mol Phylogenet Evol;118:156-171, 2018 Jan.
[Is] ISSN:1095-9513
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Marmosets and tamarins (Cebidae, Callitrichinae) constitute the most species-rich subfamily of New World monkeys and one of the most diverse phenotypically. Despite the profusion of molecular phylogenies of the group, the evolution of phenotypic characters under the rapidly-emerging consensual phylogeny of the subfamily has been little studied, resulting in taxonomic proposals that have limited support from other datasets. We examined the evolution of 18 phenotypic traits (5 continuous and 13 discrete), including pelage, skull, dentition, postcrania, life-history and vocalization variables in a robust molecular phylogeny of marmoset and tamarin monkeys, quantifying their phylogenetic signal and correlations among some of the traits. At the family level, our resulting topology supports owl monkeys (Aotinae) as sister group of Callitrichinae. The topology of the callitrichine tree was congruent with previous studies except for the position of the midas group of Saguinus tamarins, which placement as sister of the bicolor group did not receive significant statistical support in both Maximum Parsimony and Bayesian Inference analyses. Our results showed that the highest value of phylogenetic signal among continuous traits was displayed by the long call character and the lowest was exhibited in the home range, intermediate values were found in characters related to osteology and skull size. Among discrete traits, pelage and osteology had similar phylogenetic signal. Based on genetic, osteological, pelage and vocalization data, we present an updated genus-level taxonomy of Callitrichinae, which recognizes six genera in the subfamily: Callimico, Callithrix, Cebuella, Mico, Leontopithecus and Saguinus. To reflect their phenotypic distinctiveness and to avoid the use of the informal "species group", we subdivided Saguinus in the subgenera Leontocebus, Saguinus and Tamarinus (revalidated here).
[Mh] Termos MeSH primário: Evolução Biológica
Callitrichinae/classificação
[Mh] Termos MeSH secundário: Animais
Teorema de Bayes
Tamanho Corporal
Callithrix/anatomia & histologia
Callithrix/classificação
Callitrichinae/anatomia & histologia
Geografia
Comportamento de Retorno ao Território Vital
Funções Verossimilhança
Fenótipo
Filogenia
Crânio/anatomia & histologia
Vocalização Animal/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180302
[Lr] Data última revisão:
180302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171010
[St] Status:MEDLINE


  3 / 3390 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29300153
[Au] Autor:Modesto M; Michelini S; Oki K; Biavati B; Watanabe K; Mattarelli P
[Ad] Endereço:1​Department of Agricultural Sciences, University of Bologna, Italy.
[Ti] Título:Bifidobacterium catulorum sp. nov., a novel taxon from the faeces of the baby common marmoset (Callithrix jacchus).
[So] Source:Int J Syst Evol Microbiol;68(2):575-581, 2018 Feb.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In our previous study based on hsp60 PCR-restriction fragment length polymorphism and 16S rRNA gene sequencing, we stated that the bifidobacterial strains isolated from the individual faecal samples of five baby common marmosets constituted different phylogenetically isolated groups of the genus Bifidobacterium. In that study, we also proposed that these isolated groups potentially represented novel species of the genus Bifidobacterium. Out of them, Bifidobacterium aesculapii, Bifidobacterium myosotis, Bifidobacterium tissieri and Bifidobacterium hapali, have been described recently. Another strain, designated MRM 8.19 , has been classified as member of the genus Bifidobacterium on the basis of positive results for fructose-6-phosphate phosphoketolase activity and analysis of partial 16S rRNA, hsp60, clpC, dnaJ, dnaG and rpoB gene sequences. Analysis of 16S rRNA and hsp60 gene sequences revealed that strain MRM 8.19 was related to B. tissieri DSM 100201 (95.8 %) and to Bifidobacterium bifidum ATCC 29521 (93.7 %), respectively. The DNA G+C composition was 63.7 mol% and the peptidoglycan structure was l-Orn(Lys)-l-Ser. Based on the phylogenetic, genotypic and phenotypic data reported, strain MRM 8.19 represents a novel taxon within the genus Bifidobacterium for which the name Bifidobacterium catulorum sp. nov. (type strain MRM 8.19 =DSM 103154 =JCM 31794 ) is proposed.
[Mh] Termos MeSH primário: Bifidobacterium/classificação
Callithrix/microbiologia
Filogenia
[Mh] Termos MeSH secundário: Aldeído Liases/genética
Animais
Técnicas de Tipagem Bacteriana
Composição de Bases
Bifidobacterium/genética
Bifidobacterium/isolamento & purificação
Chaperonina 60/genética
DNA Bacteriano/genética
Fezes/microbiologia
Genes Bacterianos
Peptidoglicano/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chaperonin 60); 0 (DNA, Bacterial); 0 (Peptidoglycan); 0 (RNA, Ribosomal, 16S); EC 4.1.2.- (Aldehyde-Lyases); EC 4.1.2.22 (fructose-6-phosphate phosphoketolase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180105
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002545


  4 / 3390 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28957433
[Au] Autor:Ziegler TE; Sosa ME; Colman RJ
[Ad] Endereço:Wisconsin National Primate Research Center, University of Wisconsin, Madison, Wisconsin, United States of America.
[Ti] Título:Fathering style influences health outcome in common marmoset (Callithrix jacchus) offspring.
[So] Source:PLoS One;12(9):e0185695, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In the cooperative breeding common marmoset monkey, Callithrix jacchus, fathers share the care responsibility and energetic load with their mate from the time their infants are born. However, not all fathers show the same level of participation in direct infant care. Here we present the first results demonstrating that fathering style can improve both survival and growth trajectory of a male's offspring during the first 30 weeks of life and that these infant outcomes are consistent within a father throughout successive births. Twenty-four marmoset fathers were tested for their responsiveness to an infant distress call when their infants were approximately two weeks old. These fathers were categorized as either responsive (RS) or nonresponsive (NRS) based on their response to the calls. Survival past 1 month was then determined and bi-monthly weights of current infants through 30 weeks of age were taken. Infant survival to the first month was significantly higher with RS fathers than with NRS fathers during this critical time period. Infants from RS fathers also had a higher growth trajectory with significant differences in body weight in the 28th and 30th week after birth. Only the RS fathers showed a significant increase in serum testosterone in response to infant cries suggesting a physiological role of testosterone in the motivation to search for the infant. Furthermore, all offspring born to RS fathers from subsequent births also showed a significantly higher survival rate and higher growth trajectory than for offspring of NRS fathers. These results suggest that fathering style is a consistent trait and responsive fathers improve infant survival rate and growth during the first 30 weeks. Such fathering style traits may be passed on to the male offspring due to environmental or genetic factors.
[Mh] Termos MeSH primário: Comportamento Animal
Callithrix/fisiologia
[Mh] Termos MeSH secundário: Comunicação Animal
Animais
Animais Recém-Nascidos
Masculino
Testosterona/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
3XMK78S47O (Testosterone)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170929
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185695


  5 / 3390 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28915405
[Au] Autor:Manickam C; Martinot AJ; Jones RA; Varner V; Reeves RK
[Ad] Endereço:Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Boston, MA, USA.
[Ti] Título:Hepatic immunopathology during occult hepacivirus re-infection.
[So] Source:Virology;512:48-55, 2017 Dec.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Despite drug advances for Hepatitis C virus (HCV), re-infections remain prevalent in high-risk populations. Unfortunately, the role of preexisting viral immunity and how it modulates re-infection is unclear. GBV-B infection of common marmosets is a useful model to study tissue immune responses in hepacivirus infections, and in this study we re-challenged 4 animals after clearance of primary viremia. Although only low-to-absent viremia was observed following re-challenge, GBV-B viral RNA was detectable in liver, confirming re-infection. Microscopic hepatic lesions indicated severe-to-mild lymphocyte infiltration and fibrosis in 3 out of 4 animals. Further, GBV-B-specific T cells were elevated in animals with moderate-to-severe hepatopathology, and up to 3-fold increases in myeloid dendritic and activated natural killer cells were observed after infection. Our data indicate that occult hepacivirus re-infections occur and that new liver pathology is possible even in the presence of anti-hepacivirus T cells and in the absence of high viremia.
[Mh] Termos MeSH primário: Infecções por Flaviviridae/imunologia
Vírus GB B/fisiologia
Hepatite Viral Animal/imunologia
[Mh] Termos MeSH secundário: Animais
Callithrix
Infecções por Flaviviridae/patologia
Hepatite Viral Animal/patologia
Imunidade Inata/fisiologia
Fígado/patologia
Fígado/virologia
Linfócitos T/fisiologia
Carga Viral
Viremia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170916
[St] Status:MEDLINE


  6 / 3390 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28912155
[Au] Autor:Townsend RG; Solomon SS; Martin PR; Solomon SG; Gong P
[Ad] Endereço:School of Physics.
[Ti] Título:Visual Motion Discrimination by Propagating Patterns in Primate Cerebral Cortex.
[So] Source:J Neurosci;37(42):10074-10084, 2017 Oct 18.
[Is] ISSN:1529-2401
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Visual stimuli can evoke waves of neural activity that propagate across the surface of visual cortical areas. The relevance of these waves for visual processing is unknown. Here, we measured the phase and amplitude of local field potentials (LFPs) in electrode array recordings from the motion-processing medial temporal (MT) area of anesthetized male marmosets. Animals viewed grating or dot-field stimuli drifting in different directions. We found that, on individual trials, the direction of LFP wave propagation is sensitive to the direction of stimulus motion. Propagating LFP patterns are also detectable in trial-averaged activity, but the trial-averaged patterns exhibit different dynamics and behaviors from those in single trials and are similar across motion directions. We show that this difference arises because stimulus-sensitive propagating patterns are present in the phase of single-trial oscillations, whereas the trial-averaged signal is dominated by additive amplitude effects. Our results demonstrate that propagating LFP patterns can represent sensory inputs at timescales relevant to visually guided behaviors and raise the possibility that propagating activity patterns serve neural information processing in area MT and other cortical areas. Propagating wave patterns are widely observed in the cortex, but their functional relevance remains unknown. We show here that visual stimuli generate propagating wave patterns in local field potentials (LFPs) in a movement-sensitive area of the primate cortex and that the propagation direction of these patterns is sensitive to stimulus motion direction. We also show that averaging LFP signals across multiple stimulus presentations (trial averaging) yields propagating patterns that capture different dynamic properties of the LFP response and show negligible direction sensitivity. Our results demonstrate that sensory stimuli can modulate propagating wave patterns reliably in the cortex. The relevant dynamics are normally masked by trial averaging, which is a conventional step in LFP signal processing.
[Mh] Termos MeSH primário: Córtex Cerebral/fisiologia
Percepção de Movimento/fisiologia
Reconhecimento Visual de Modelos/fisiologia
Estimulação Luminosa/métodos
[Mh] Termos MeSH secundário: Animais
Callithrix
Potenciais Evocados Visuais/fisiologia
Masculino
Córtex Visual/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171027
[Lr] Data última revisão:
171027
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170916
[St] Status:MEDLINE
[do] DOI:10.1523/JNEUROSCI.1538-17.2017


  7 / 3390 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28854243
[Au] Autor:Michel A; Nicolas JM; Rose S; Jackson M; Colman P; Briône W; Sciberras D; Muglia P; Scheller DK; Citron M; Downey P
[Ad] Endereço:UCB BioPharma, Braine L'Alleud, Belgium.
[Ti] Título:Antiparkinsonian effects of the "Radiprodil and Tozadenant" combination in MPTP-treated marmosets.
[So] Source:PLoS One;12(8):e0182887, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: Investigate a combination of two clinically tested drugs, the NR2B antagonist Radiprodil and the A2A antagonist Tozadenant in the MPTP-treated marmoset model of Parkinson's Disease (PD). BACKGROUND: In PD, there remains a need for the development of non-dopaminergic drugs to effectively treat the motor symptoms without the induction of L-Dopa-induced motor complications. METHODS: Clinically relevant doses of Radiprodil and Tozadenant were given both alone and in combination without the addition of L-Dopa, and the antiparkinsonian efficacy of the treatments was assessed in a primate model of PD. RESULTS: When compared to the drugs tested alone, the drug combination led to a significant increase of motor activity and an improvement of motor disability in MPTP-treated marmosets. In addition, the motor restoration brought about by the combination was almost completely devoid of dyskinesia. Interestingly, treated primates were not overstimulated, but were able to move normally when motivated by the exploration of novel objects. CONCLUSION: We have demonstrated in a primate model that, the "Radiprodil/Tozadenant" combination significantly improves motor activity, extending previous results obtained in unilaterally lesioned 6-OHDA-rats. The strength of the preclinical data accumulated so far suggests that the use of such an A2A and NR2B antagonist combination could bring significant motor improvement to PD patients, without inducing the motor complications induced by L-Dopa therapy. Although encouraging, these preclinical data need to be confirmed in the clinic.
[Mh] Termos MeSH primário: Antiparkinsonianos/farmacologia
Benzotiazóis/farmacologia
Intoxicação por MPTP/tratamento farmacológico
Atividade Motora/efeitos dos fármacos
Receptores A2 de Adenosina/genética
Receptores de N-Metil-D-Aspartato/antagonistas & inibidores
[Mh] Termos MeSH secundário: Animais
Callithrix
Esquema de Medicação
Combinação de Medicamentos
Avaliação Pré-Clínica de Medicamentos
Sinergismo Farmacológico
Discinesia Induzida por Medicamentos/prevenção & controle
Feminino
Expressão Gênica
Intoxicação por MPTP/genética
Intoxicação por MPTP/metabolismo
Intoxicação por MPTP/fisiopatologia
Masculino
Atividade Motora/fisiologia
Receptores A2 de Adenosina/metabolismo
Receptores de N-Metil-D-Aspartato/genética
Receptores de N-Metil-D-Aspartato/metabolismo
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antiparkinson Agents); 0 (Benzothiazoles); 0 (Drug Combinations); 0 (NR2B NMDA receptor); 0 (Receptors, Adenosine A2); 0 (Receptors, N-Methyl-D-Aspartate); D9K857J81I (tozadenant)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170831
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182887


  8 / 3390 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28841690
[Au] Autor:Kücklich M; Möller M; Marcillo A; Einspanier A; Weiß BM; Birkemeyer C; Widdig A
[Ad] Endereço:Junior Research Group of Primate Kin Selection, Department of Primatology, Max-Planck-Institute for Evolutionary Anthropology, Leipzig, Germany.
[Ti] Título:Different methods for volatile sampling in mammals.
[So] Source:PLoS One;12(8):e0183440, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Previous studies showed that olfactory cues are important for mammalian communication. However, many specific compounds that convey information between conspecifics are still unknown. To understand mechanisms and functions of olfactory cues, olfactory signals such as volatile compounds emitted from individuals need to be assessed. Sampling of animals with and without scent glands was typically conducted using cotton swabs rubbed over the skin or fur and analysed by gas chromatography-mass spectrometry (GC-MS). However, this method has various drawbacks, including a high level of contaminations. Thus, we adapted two methods of volatile sampling from other research fields and compared them to sampling with cotton swabs. To do so we assessed the body odor of common marmosets (Callithrix jacchus) using cotton swabs, thermal desorption (TD) tubes and, alternatively, a mobile GC-MS device containing a thermal desorption trap. Overall, TD tubes comprised most compounds (N = 113), with half of those compounds being volatile (N = 52). The mobile GC-MS captured the fewest compounds (N = 35), of which all were volatile. Cotton swabs contained an intermediate number of compounds (N = 55), but very few volatiles (N = 10). Almost all compounds found with the mobile GC-MS were also captured with TD tubes (94%). Hence, we recommend TD tubes for state of the art sampling of body odor of mammals or other vertebrates, particularly for field studies, as they can be easily transported, stored and analysed with high performance instruments in the lab. Nevertheless, cotton swabs capture compounds which still may contribute to the body odor, e.g. after bacterial fermentation, while profiles from mobile GC-MS include only the most abundant volatiles of the body odor.
[Mh] Termos MeSH primário: Callithrix/fisiologia
Compostos Orgânicos Voláteis/análise
[Mh] Termos MeSH secundário: Comunicação Animal
Animais
Feminino
Cromatografia Gasosa-Espectrometria de Massas
Odorantes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Volatile Organic Compounds)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170826
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183440


  9 / 3390 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Antunes, Edson
Texto completo
[PMID]:28837636
[Au] Autor:Campos R; Mónica FZ; Rodrigues RL; Rojas-Moscoso JA; Moreno RA; Cogo JC; de Oliveira MA; Antunes E; De Nucci G
[Ad] Endereço:Faculty of Medical Sciences, Department of Pharmacology, University of Campinas (UNICAMP), Campinas, Brazil.
[Ti] Título:Tetrodotoxin-insensitive electrical field stimulation-induced contractions on Crotalus durissus terrificus corpus cavernosum.
[So] Source:PLoS One;12(8):e0183766, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Reptiles are the first amniotes to develop an intromitent penis, however until now the mechanisms involved in the electrical field stimulation-induced contraction on corpora cavernosa isolated from Crotalus durissus terrificus were not investigated. Crotalus and rabbit corpora cavernosa were mounted in 10 mL organ baths for isometric tension recording. Electrical field stimulation (EFS)-induced contractions were performed in presence/absence of phentolamine (10 µM), guanethidine (30 µM), tetrodotoxin (1 µM and 1mM), A-803467 (10 µM), 3-iodo-L-Tyrosine (1 mM), salsolinol (3 µM) and a modified Krebs solution (equimolar substitution of NaCl by N-methyl-D-glucamine). Immuno-histochemistry for tyrosine hydroxylase was also performed. Electrical field stimulation (EFS; 8 Hz and 16 Hz) caused contractions in both Crotalus and rabbit corpora cavernosa. The contractions were abolished by previous incubation with either phentolamine or guanethidine. Tetrodotoxin (1 µM) also abolished the EFS-induced contractions of rabbit CC, but did not affect EFS-induced contractions of Crotalus CC. Addition of A-803467 (10 µM) did not change the EFS-induced contractions of Crotalus CC but abolished rabbit CC contractions. 3-iodo-L-Tyrosine and salsolinol had no effect on EFS-induced contractions of Crotalus CC and Rabbit CC. Replacement of NaCl by N- Methyl-D-glucamine (NMDG) abolished EFS-induced contractions of rabbit CC, but did not affect Crotalus CC. The presence of tyrosine hydroxylase was identified in endothelial cells only of Crotalus CC. Since the EFS-induced contractions of Crotalus CC is dependent on catecholamine release, insensitive to TTX, insensitive to A803467 and to NaCl replacement, it indicates that the source of cathecolamine is unlikely to be from adrenergic terminals. The finding that tyrosine hydroxylase is present in endothelial cells suggests that these cells can modulate Crotalus CC tone.
[Mh] Termos MeSH primário: Crotalus/fisiologia
Estimulação Elétrica
Pênis/efeitos dos fármacos
Tetrodotoxina/farmacologia
[Mh] Termos MeSH secundário: Compostos de Anilina/farmacologia
Animais
Callithrix
Furanos/farmacologia
Imuno-Histoquímica
Masculino
Contração Muscular/efeitos dos fármacos
Pênis/fisiologia
Coelhos
Receptores Adrenérgicos/fisiologia
Canais de Sódio/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (A 803467); 0 (Aniline Compounds); 0 (Furans); 0 (Receptors, Adrenergic); 0 (Sodium Channels); 4368-28-9 (Tetrodotoxin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170825
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183766


  10 / 3390 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28802219
[Au] Autor:Gaudette F; Hamadjida A; Bédard D; Nuara SG; Beaudry F; Huot P
[Ad] Endereço:CRCHUM, Centre de Recherche du Centre Hospitalier de l'Université de Montréal, Montreal, Quebec, Canada. Electronic address: fleur.gaudette.chum@ssss.gouv.qc.ca.
[Ti] Título:Development and validation of a high-performance liquid chromatography-tandem mass spectrometry method to quantify LY-354,740 in rat and marmoset plasma.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1061-1062:392-398, 2017 Sep 01.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:LY-354,740 (eglumegad) is a selective and potent agonist of the metabotropic glutamate group II receptors (mGluR2,3) that has already entered clinical trials as a potential anti-psychotic agent and therefore has well-documented pharmacokinetic (PK), safety and tolerability profiles in human. Whereas its development as an anti-psychotic agent has not been pursued, LY-354,740 may have potential in other neuroscience-related fields, notably anxiety and neuro-protection. The common marmoset is a small primate that has long been used in neuroscience. However, given its small size and small circulating blood volume, conducting PK studies to determine the therapeutic effectiveness of LY-354,740 at clinically-relevant doses is challenging. Here, we have developed and validated a simple, sensitive and selective analytical method that enables quantification of LY-354,740 using a small volume of plasma. The analytical method consisted of protein precipitation followed by high-performance liquid chromatography with heat assisted electrospray ionisation mass spectrometry (UHPLC-HESI-MS/MS). The chromatographic separation was achieved using gradient elution with a mobile phase consisting of acetonitrile and 10mM ammonium formate (pH 3) on a Thermo Scientific Acclaim Trinity P1 analytical column (100x3.0mm I.D., 3µm) operating at 45°C and at a flow rate of 900µl/min. The method displays a linear relationship ranging from 20.0 to 5000ng/ml. Intra- and inter-day relative standard deviations are less than 5.7% and 7.0%, respectively and the accuracy ranged from 91.0 to 106.0%. The UHPLC-HESI-MS/MS analytical method we describe here is simple, sensitive, specific and capable of quantifying LY-354,740 in both rat and marmoset plasma, and is suitable to conduct PK studies after a single sub-cutaneous dose of 1.0mg/kg or lower in both species.
[Mh] Termos MeSH primário: Compostos Bicíclicos com Pontes/sangue
Cromatografia Líquida de Alta Pressão/métodos
Espectrometria de Massas em Tandem/métodos
[Mh] Termos MeSH secundário: Animais
Compostos Bicíclicos com Pontes/química
Compostos Bicíclicos com Pontes/farmacocinética
Callithrix
Estabilidade de Medicamentos
Limite de Detecção
Modelos Lineares
Ratos
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bridged Bicyclo Compounds); ONU5A67T2S (eglumetad)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170813
[St] Status:MEDLINE



página 1 de 339 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde