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  1 / 9782 MEDLINE  
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[PMID]:28454881
[Au] Autor:Foroutan M; Khademvatan S; Majidiani H; Khalkhali H; Hedayati-Rad F; Khashaveh S; Mohammadzadeh H
[Ad] Endereço:Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran; Student Research Committee, Urmia University of Medical Sciences, Urmia, Iran. Electronic address: m.foroutan@modares.ac.ir.
[Ti] Título:Prevalence of Leishmania species in rodents: A systematic review and meta-analysis in Iran.
[So] Source:Acta Trop;172:164-172, 2017 Aug.
[Is] ISSN:1873-6254
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Leishmaniasis are diverse group of diseases caused by numerous species of genus Leishmania. Herein we have contrived a systematic review and meta-analysis on the prevalence of Leishmania species in rodents of Iran. For this purpose, following the general methodology recommended for systematic reviews and meta-analysis, six English databases (PubMed, Science Direct, Scopus, Ovid, Web of Science and Google Scholar) and four Persian databases (Magiran, SID, Iran Doc and Iran Medex) were explored during January 1995 till June 2015. Papers were selected based on 8 pre-defined inclusion criteria. During the years, a total number of 4485 different rodents were captured; among which 1291 cases were Leishmania positive. The calculated weighted prevalence of Leishmania species in rodents was 23% (95% CI=18-28). Given geographical zones of Iran, the highest and lowest prevalence rate was belonged to North 50% (95% CI=40-61) and West 11% (95% CI=5-17), respectively. Rhombomys opimus (1766), Meriones lybicus (1258) and Tatera indica (488) were the three most abundant captured rodents, while the highest prevalence of Leishmania species was observed in Nesokia indica 48% (95% CI=42-54) and followed by R. opimus 39% (95% CI=30-47). Egger's regression test was performed to detect publication bias, which revealed it may not have a significant influence on overall weighted prevalence estimate (P=0.317). Meta-regression analysis demonstrated that there is no significant relationship between overall prevalence with sample size (P=0.1) and year of publication (P=0.7). The results showed remarkable prevalence of Leishmania species in rodent reservoirs. In future, adopting a suitable strategy for control and combat with rodents is necessary.
[Mh] Termos MeSH primário: Gerbillinae
Leishmania/isolamento & purificação
Leishmaniose Cutânea/veterinária
Doenças dos Roedores/parasitologia
[Mh] Termos MeSH secundário: Animais
Irã (Geográfico)/epidemiologia
Leishmania/classificação
Leishmaniose Cutânea/epidemiologia
Leishmaniose Cutânea/parasitologia
Prevalência
Doenças dos Roedores/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; META-ANALYSIS; REVIEW
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180202
[Lr] Data última revisão:
180202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE


  2 / 9782 MEDLINE  
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[PMID]:28902909
[Au] Autor:Vange P; Bruland T; Doseth B; Fossmark R; Sousa MML; Beisvag V; Sørdal Ø; Qvigstad G; Waldum HL; Sandvik AK; Bakke I
[Ad] Endereço:Department of Clinical and Molecular Medicine, NTNU, Norwegian University of Science and Technology, Trondheim, Norway.
[Ti] Título:The cytoprotective protein clusterin is overexpressed in hypergastrinemic rodent models of oxyntic preneoplasia and promotes gastric cancer cell survival.
[So] Source:PLoS One;12(9):e0184514, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The cytoprotective protein clusterin is often dysregulated during tumorigenesis, and in the stomach, upregulation of clusterin marks emergence of the oxyntic atrophy (loss of acid-producing parietal cells)-associated spasmolytic polypeptide-expressing metaplasia (SPEM). The hormone gastrin is important for normal function and maturation of the gastric oxyntic mucosa and hypergastrinemia might be involved in gastric carcinogenesis. Gastrin induces expression of clusterin in adenocarcinoma cells. In the present study, we examined the expression patterns and gastrin-mediated regulation of clusterin in gastric tissue from: humans; rats treated with proton pump (H+/K+-ATPase) inhibitors and/or a gastrin receptor (CCK2R) antagonist; H+/K+-ATPase ß-subunit knockout (H/K-ß KO) mice; and Mongolian gerbils infected with Helicobacter pylori and given a CCK2R antagonist. Biological function of secretory clusterin was studied in human gastric cancer cells. Clusterin was highly expressed in neuroendocrine cells in normal oxyntic mucosa of humans and rodents. In response to hypergastrinemia, expression of clusterin increased significantly and its localization shifted to basal groups of proliferative cells in the mucous neck cell-chief cell lineage in all animal models. That shift was partially inhibited by antagonizing the CCK2R in rats and gerbils. The oxyntic mucosa of H/K-ß KO mice contained areas with clusterin-positive mucous cells resembling SPEM. In gastric adenocarcinomas, clusterin mRNA expression was higher in diffuse tumors containing signet ring cells compared with diffuse tumors without signet ring cells, and clusterin seemed to be secreted by tumor cells. In gastric cancer cell lines, gastrin increased secretion of clusterin, and both gastrin and secretory clusterin promoted survival after starvation- and chemotherapy-induced stress. Overall, our results indicate that clusterin is overexpressed in hypergastrinemic rodent models of oxyntic preneoplasia and stimulates gastric cancer cell survival.
[Mh] Termos MeSH primário: Clusterina/fisiologia
Regulação Neoplásica da Expressão Gênica
Células Parietais Gástricas/patologia
Neoplasias Gástricas/patologia
[Mh] Termos MeSH secundário: Idoso
Idoso de 80 Anos ou mais
Animais
Carcinogênese/genética
Carcinogênese/metabolismo
Linhagem Celular Tumoral
Clusterina/genética
Clusterina/metabolismo
Feminino
Gastrinas/metabolismo
Gastrinas/fisiologia
Perfilação da Expressão Gênica
Gerbillinae
Seres Humanos
Masculino
Camundongos Knockout
Meia-Idade
Células Parietais Gástricas/metabolismo
Inibidores da Bomba de Prótons/farmacologia
Ratos
Ratos Sprague-Dawley
Receptor de Colecistocinina B/antagonistas & inibidores
Neoplasias Gástricas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Clusterin); 0 (Gastrins); 0 (Proton Pump Inhibitors); 0 (Receptor, Cholecystokinin B)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170914
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184514


  3 / 9782 MEDLINE  
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[PMID]:28771568
[Au] Autor:Schaefer MK; Kössl M; Hechavarría JC
[Ad] Endereço:Institute for Cell Biology and Neuroscience, AK Neurobiology and Biosensorics, Goethe University, Frankfurt/Main, Germany.
[Ti] Título:Laminar differences in response to simple and spectro-temporally complex sounds in the primary auditory cortex of ketamine-anesthetized gerbils.
[So] Source:PLoS One;12(8):e0182514, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In mammals, acoustic communication plays an important role during social behaviors. Despite their ethological relevance, the mechanisms by which the auditory cortex represents different communication call properties remain elusive. Recent studies have pointed out that communication-sound encoding could be based on discharge patterns of neuronal populations. Following this idea, we investigated whether the activity of local neuronal networks, such as those occurring within individual cortical columns, is sufficient for distinguishing between sounds that differed in their spectro-temporal properties. To accomplish this aim, we analyzed simple pure-tone and complex communication call elicited multi-unit activity (MUA) as well as local field potentials (LFP), and current source density (CSD) waveforms at the single-layer and columnar level from the primary auditory cortex of anesthetized Mongolian gerbils. Multi-dimensional scaling analysis was used to evaluate the degree of "call-specificity" in the evoked activity. The results showed that whole laminar profiles segregated 1.8-2.6 times better across calls than single-layer activity. Also, laminar LFP and CSD profiles segregated better than MUA profiles. Significant differences between CSD profiles evoked by different sounds were more pronounced at mid and late latencies in the granular and infragranular layers and these differences were based on the absence and/or presence of current sinks and on sink timing. The stimulus-specific activity patterns observed within cortical columns suggests that the joint activity of local cortical populations (as local as single columns) could indeed be important for encoding sounds that differ in their acoustic attributes.
[Mh] Termos MeSH primário: Estimulação Acústica/métodos
Córtex Auditivo/fisiologia
Gerbillinae/fisiologia
[Mh] Termos MeSH secundário: Animais
Fenômenos Eletrofisiológicos
Potenciais Evocados Auditivos
Ketamina/administração & dosagem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
690G0D6V8H (Ketamine)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170829
[Lr] Data última revisão:
170829
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170804
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182514


  4 / 9782 MEDLINE  
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[PMID]:28706081
[Au] Autor:Green DB; Mattingly MM; Ye Y; Gay JD; Rosen MJ
[Ad] Endereço:Department of Anatomy & Neurobiology, Northeast Ohio Medical University, Rootstown, Ohio 44272.
[Ti] Título:Brief Stimulus Exposure Fully Remediates Temporal Processing Deficits Induced by Early Hearing Loss.
[So] Source:J Neurosci;37(32):7759-7771, 2017 Aug 09.
[Is] ISSN:1529-2401
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In childhood, partial hearing loss can produce prolonged deficits in speech perception and temporal processing. However, early therapeutic interventions targeting temporal processing may improve later speech-related outcomes. Gap detection is a measure of auditory temporal resolution that relies on the auditory cortex (ACx), and early auditory deprivation alters intrinsic and synaptic properties in the ACx. Thus, early deprivation should induce deficits in gap detection, which should be reflected in ACx gap sensitivity. We tested whether earplugging-induced, early transient auditory deprivation in male and female Mongolian gerbils caused correlated deficits in behavioral and cortical gap detection, and whether these could be rescued by a novel therapeutic approach: brief exposure to gaps in background noise. Two weeks after earplug removal, animals that had been earplugged from hearing onset throughout auditory critical periods displayed impaired behavioral gap detection thresholds (GDTs), but this deficit was fully reversed by three 1 h sessions of exposure to gaps in noise. In parallel, after earplugging, cortical GDTs increased because fewer cells were sensitive to short gaps, and gap exposure normalized this pattern. Furthermore, in deprived animals, both first-spike latency and first-spike latency jitter increased, while spontaneous and evoked firing rates decreased, suggesting that deprivation causes a wider range of perceptual problems than measured here. These cortical changes all returned to control levels after gap exposure. Thus, brief stimulus exposure, perhaps in a salient context such as the unfamiliar placement into a testing apparatus, rescued impaired gap detection and may have potential as a remediation tool for general auditory processing deficits. Hearing loss in early childhood leads to impairments in auditory perception and language processing that can last well beyond the restoration of hearing sensitivity. Perceptual deficits can be improved by training, or by acoustic enrichment in animal models, but both approaches involve extended time and effort. Here, we used a novel remediation technique, brief periods of auditory stimulus exposure, to fully remediate cortical and perceptual deficits in gap detection induced by early transient hearing loss. This technique also improved multiple cortical response properties. Rescue by this efficient exposure regime may have potential as a therapeutic tool to remediate general auditory processing deficits in children with perceptual challenges arising from early hearing loss.
[Mh] Termos MeSH primário: Estimulação Acústica/métodos
Córtex Auditivo/fisiologia
Córtex Auditivo/fisiopatologia
Percepção Auditiva/fisiologia
Potenciais Evocados Auditivos do Tronco Encefálico/fisiologia
Perda Auditiva/fisiopatologia
[Mh] Termos MeSH secundário: Fatores Etários
Animais
Feminino
Gerbillinae
Masculino
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170911
[Lr] Data última revisão:
170911
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170715
[St] Status:MEDLINE
[do] DOI:10.1523/JNEUROSCI.0916-17.2017


  5 / 9782 MEDLINE  
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Taboga, Sebastiäo Roberto
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[PMID]:28664583
[Au] Autor:Antoniassi JQ; Fochi RA; Góes RM; Vilamaior PSL; Taboga SR
[Ad] Endereço:Department of Structural and Functional Biology, Institute of Biology, State University of Campinas (UNICAMP), São Paulo, Brazil.
[Ti] Título:Corticosterone influences gerbil (Meriones unguiculatus) prostatic morphophysiology and alters its proliferation and apoptosis rates.
[So] Source:Int J Exp Pathol;98(3):134-146, 2017 Jun.
[Is] ISSN:1365-2613
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Glucocorticoids (GCs) are hormones that are widely used in medicine; but although side effects are generally recognised, little is known about the precise mechanisms that is implicated in many of these side effects. Furthermore, GCs are highly correlated with stress and behaviour disorders. This study evaluated the effects of the glucocorticoid corticosterone on the ventral prostate of the Mongolian gerbil. Male gerbils (Meriones unguiculatus) (n = 5) received intraperitoneal injections of saline or corticosterone in doses of 0.5 mg/kg/day and 1.5 mg/kg/day for 5 days; while some of the animals were killed immediately after the treatment, the others were killed 5 days after the treatment period. The data show that corticosterone influences the structure and functionality of this organ. This hormone has anti-proliferative and anti-apoptotic properties in the prostate. In addition, the frequencies of the androgen (AR), oestrogen (ERα, ERß) and glucocorticoid (GR) receptors changed. The frequencies of AR, GR and ERß decreased in the Ct1/5 group; in the groups with rest period, the frequencies of GR increased and ERß decreased in the epithelium. Changes in the proliferative index, apoptotic index and receptor activity may have contributed to the emergence of prostatic morphological alterations, such as the presence of cellular debris and inflammatory cells. Different doses of corticosterone had variable effects on the prostate, with a higher dose showing subtler effects and a lower dose showing more striking effects. The corticosterone effects on nuclear receptors were reverted or attenuated after a rest period, which was not observed for proliferation and apoptosis. In summary, we have demonstrated that corticosterone might influence the prostatic morphophysiology and that these changes may be linked in some way to the altered receptor distribution.
[Mh] Termos MeSH primário: Corticosterona/farmacologia
Próstata/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Apoptose/efeitos dos fármacos
Proliferação Celular/efeitos dos fármacos
Corticosterona/administração & dosagem
Corticosterona/sangue
Relação Dose-Resposta a Droga
Gerbillinae
Masculino
Tamanho do Órgão/efeitos dos fármacos
Próstata/metabolismo
Próstata/patologia
Receptores Androgênicos/metabolismo
Receptores Estrogênicos/metabolismo
Receptores de Glucocorticoides/metabolismo
Testosterona/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Receptors, Androgen); 0 (Receptors, Estrogen); 0 (Receptors, Glucocorticoid); 3XMK78S47O (Testosterone); W980KJ009P (Corticosterone)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170701
[St] Status:MEDLINE
[do] DOI:10.1111/iep.12232


  6 / 9782 MEDLINE  
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[PMID]:28659280
[Au] Autor:Plauska A; van der Heijden M; Borst JGG
[Ad] Endereço:Department of Neuroscience, Erasmus MC, University Medical Center Rotterdam, 3000 CA, Rotterdam, The Netherlands.
[Ti] Título:A Test of the Stereausis Hypothesis for Sound Localization in Mammals.
[So] Source:J Neurosci;37(30):7278-7289, 2017 Jul 26.
[Is] ISSN:1529-2401
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The relative arrival times of sounds at both ears constitute an important cue for localization of low-frequency sounds in the horizontal plane. The binaural neurons of the medial superior olive (MSO) act as coincidence detectors that fire when inputs from both ears arrive near simultaneously. Each principal neuron in the MSO is tuned to its own best interaural time difference (ITD), indicating the presence of an internal delay, a difference in the travel times from either ear to the MSO. According to the stereausis hypothesis, differences in wave propagation along the cochlea could provide the delays necessary for coincidence detection if the ipsilateral and contralateral inputs originated from different cochlear positions, with different frequency tuning. We therefore investigated the relation between interaural mismatches in frequency tuning and ITD tuning during loose-patch (juxtacellular) recordings from principal neurons of the MSO of anesthetized female gerbils. Cochlear delays can be bypassed by directly stimulating the auditory nerve; in agreement with the stereausis hypothesis, tuning for timing differences during bilateral electrical stimulation of the round windows differed markedly from ITD tuning in the same cells. Moreover, some neurons showed a frequency tuning mismatch that was sufficiently large to have a potential impact on ITD tuning. However, we did not find a correlation between frequency tuning mismatches and best ITDs. Our data thus suggest that axonal delays dominate ITD tuning. Neurons in the medial superior olive (MSO) play a unique role in sound localization because of their ability to compare the relative arrival time of low-frequency sounds at both ears. They fire maximally when the difference in sound arrival time exactly compensates for the internal delay: the difference in travel time from either ear to the MSO neuron. We tested whether differences in cochlear delay systematically contribute to the total travel time by comparing for individual MSO neurons the best difference in arrival times, as predicted from the frequency tuning for either ear, and the actual best difference. No systematic relation was observed, emphasizing the dominant contribution of axonal delays to the internal delay.
[Mh] Termos MeSH primário: Vias Auditivas/fisiologia
Cóclea/fisiologia
Modelos Neurológicos
Condução Nervosa/fisiologia
Células Receptoras Sensoriais/fisiologia
Localização de Som/fisiologia
Complexo Olivar Superior/fisiologia
[Mh] Termos MeSH secundário: Animais
Simulação por Computador
Feminino
Gerbillinae
Percepção do Tempo/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170822
[Lr] Data última revisão:
170822
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170630
[St] Status:MEDLINE
[do] DOI:10.1523/JNEUROSCI.0233-17.2017


  7 / 9782 MEDLINE  
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[PMID]:28541836
[Au] Autor:Alsarraf M; Mohallal EME; Mierzejewska EJ; Behnke-Borowczyk J; Welc-Faleciak R; Bednarska M; Dziewit L; Zalat S; Gilbert F; Behnke JM; Bajer A
[Ad] Endereço:1 Department of Parasitology, Faculty of Biology, Institute of Zoology, University of Warsaw , Warsaw, Poland .
[Ti] Título:Description of Candidatus Bartonella fadhilae n. sp. and Candidatus Bartonella sanaae n. sp. (Bartonellaceae) from Dipodillus dasyurus and Sekeetamys calurus (Gerbillinae) from the Sinai Massif (Egypt).
[So] Source:Vector Borne Zoonotic Dis;17(7):483-494, 2017 Jul.
[Is] ISSN:1557-7759
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bartonella spp. are parasites of mammalian erythrocytes and endothelial cells, transmitted by blood-feeding arthropod ectoparasites. Different species of rodents may constitute the main hosts of Bartonella, including several zoonotic species of Bartonella. The aim of this study was to identify and compare Bartonella species and genotypes isolated from rodent hosts from the South Sinai, Egypt. Prevalence of Bartonella infection was assessed in rodents (837 Acomys dimidiatus, 73 Acomys russatus, 111 Dipodillus dasyurus, and 65 Sekeetamys calurus) trapped in 2000, 2004, 2008, and 2012 in four dry montane wadis around St. Katherine town in the Sinai Mountains. Total DNA was extracted from blood samples, and PCR amplification and sequencing of the Bartonella-specific 860-bp gene fragment of rpoB and the 810-bp gene fragment of gltA were used for molecular and phylogenetic analyses. The overall prevalence of Bartonella in rodents was 7.2%. Prevalence differed between host species, being 30.6%, 10.8%, 9.6%, and 3.6% in D. dasyurus, S. calurus, A. russatus, and A. dimidiatus, respectively. The phylogenetic analyses of six samples of Bartonella (five from D. dasyurus and one from S. calurus) based on a fragment of the rpoB gene, revealed the existence of two distinct genetic groups (with 95-96% reciprocal sequence identity), clustering with several unidentified isolates obtained earlier from the same rodent species, and distant from species that have already been described (90-92% of sequence identity to the closest match from the GenBank reference database). Thus, molecular and phylogenetic analyses led to the description of two species: Candidatus Bartonella fadhilae n. sp. and Candidatus Bartonella sanaae n. sp. The identification of their vectors and the medical significance of these species need further investigation.
[Mh] Termos MeSH primário: Infecções por Bartonella/veterinária
Bartonella/classificação
Gerbillinae/microbiologia
[Mh] Termos MeSH secundário: Animais
Bartonella/genética
Bartonella/isolamento & purificação
Infecções por Bartonella/epidemiologia
Infecções por Bartonella/microbiologia
DNA Bacteriano
Egito/epidemiologia
Genótipo
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170921
[Lr] Data última revisão:
170921
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170526
[St] Status:MEDLINE
[do] DOI:10.1089/vbz.2016.2093


  8 / 9782 MEDLINE  
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[PMID]:28520724
[Au] Autor:Ritler D; Rufener R; Sager H; Bouvier J; Hemphill A; Lundström-Stadelmann B
[Ad] Endereço:Institute of Parasitology, Vetsuisse Faculty, University of Berne, Berne, Switzerland.
[Ti] Título:Development of a movement-based in vitro screening assay for the identification of new anti-cestodal compounds.
[So] Source:PLoS Negl Trop Dis;11(5):e0005618, 2017 May.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Intestinal cestodes are infecting millions of people and livestock worldwide, but treatment is mainly based on one drug: praziquantel. The identification of new anti-cestodal compounds is hampered by the lack of suitable screening assays. It is difficult, or even impossible, to evaluate drugs against adult cestodes in vitro due to the fact that these parasites cannot be cultured in microwell plates, and adult and larval stages in most cases represent different organisms in terms of size, morphology, and metabolic requirements. We here present an in vitro-drug screening assay based on Echinococcus multilocularis protoscoleces, which represent precursors of the scolex (hence the anterior part) of the adult tapeworm. This movement-based assay can serve as a model for an adult cestode screen. Protoscoleces are produced in large numbers in Mongolian gerbils and mice, their movement is measured and quantified by image analysis, and active compounds are directly assessed in terms of morphological effects. The use of the 384-well format minimizes the amount of parasites and compounds needed and allows rapid screening of a large number of chemicals. Standard drugs showed the expected dose-dependent effect on movement and morphology of the protoscoleces. Interestingly, praziquantel inhibited movement only partially within 12 h of treatment (at concentrations as high as 100 ppm) and did thus not act parasiticidal, which was also confirmed by trypan blue staining. Enantiomers of praziquantel showed a clear difference in their minimal inhibitory concentration in the motility assay and (R)-(-)-praziquantel was 185 times more active than (S)-(-)-praziquantel. One compound named MMV665807, which was obtained from the open access MMV (Medicines for Malaria Venture) Malaria box, strongly impaired motility and viability of protoscoleces. Corresponding morphological alterations were visualized by scanning electron microscopy, and demonstrated that this compound exhibits a mode of action clearly distinct from praziquantel. Thus, MMV665807 represents an interesting lead for further evaluation.
[Mh] Termos MeSH primário: Anti-Helmínticos/farmacologia
Bioensaio/métodos
Avaliação Pré-Clínica de Medicamentos/métodos
Echinococcus multilocularis/efeitos dos fármacos
Echinococcus multilocularis/fisiologia
Locomoção/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Benzamidas/farmacologia
Echinococcus multilocularis/anatomia & histologia
Gerbillinae
Ensaios de Triagem em Larga Escala/métodos
Processamento de Imagem Assistida por Computador/métodos
Camundongos
Testes de Sensibilidade Microbiana
Microscopia Eletrônica de Varredura
Imagem Óptica/métodos
Praziquantel/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (5-chloro-2-hydroxy-N-(3-(trifluoromethyl)phenyl)benzamide); 0 (Anthelmintics); 0 (Benzamides); 6490C9U457 (Praziquantel)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170519
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005618


  9 / 9782 MEDLINE  
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[PMID]:28420418
[Au] Autor:Mitutsova V; Yeo WWY; Davaze R; Franckhauser C; Hani EH; Abdullah S; Mollard P; Schaeffer M; Fernandez A; Lamb NJC
[Ad] Endereço:Mammalian Cell Biology group, IGH CNRS, UM, UMR 9002, 141 rue de la Cardonille, 34396, Montpellier cedex 05, France.
[Ti] Título:Adult muscle-derived stem cells engraft and differentiate into insulin-expressing cells in pancreatic islets of diabetic mice.
[So] Source:Stem Cell Res Ther;8(1):86, 2017 Apr 18.
[Is] ISSN:1757-6512
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Pancreatic beta cells are unique effectors in the control of glucose homeostasis and their deficiency results in impaired insulin production leading to severe diabetic diseases. Here, we investigated the potential of a population of nonadherent muscle-derived stem cells (MDSC) from adult mouse muscle to differentiate in vitro into beta cells when transplanted as undifferentiated stem cells in vivo to compensate for beta-cell deficiency. RESULTS: In vitro, cultured MDSC spontaneously differentiated into insulin-expressing islet-like cell clusters as revealed using MDSC from transgenic mice expressing GFP or mCherry under the control of an insulin promoter. Differentiated clusters of beta-like cells co-expressed insulin with the transcription factors Pdx1, Nkx2.2, Nkx6.1, and MafA, and secreted significant levels of insulin in response to glucose challenges. In vivo, undifferentiated MDSC injected into streptozotocin (STZ)-treated mice engrafted within 48 h specifically to damaged pancreatic islets and were shown to differentiate and express insulin 10-12 days after injection. In addition, injection of MDSC into hyperglycemic diabetic mice reduced their blood glucose levels for 2-4 weeks. CONCLUSION: These data show that MDSC are capable of differentiating into mature pancreatic beta islet-like cells, not only upon culture in vitro, but also in vivo after systemic injection in STZ-induced diabetic mouse models. Being nonteratogenic, MDSC can be used directly by systemic injection, and this potential reveals a promising alternative avenue in stem cell-based treatment of beta-cell deficiencies.
[Mh] Termos MeSH primário: Células-Tronco Adultas/citologia
Diferenciação Celular
Diabetes Mellitus Experimental/terapia
Células Secretoras de Insulina/citologia
Fatores de Transcrição Maf Maior
Fibras Musculares Esqueléticas/citologia
Transplante de Células-Tronco
[Mh] Termos MeSH secundário: Células-Tronco Adultas/metabolismo
Animais
Linhagem Celular Tumoral
Células Cultivadas
Gerbillinae
Proteínas de Homeodomínio/genética
Proteínas de Homeodomínio/metabolismo
Células Secretoras de Insulina/metabolismo
Fatores de Transcrição Maf Maior/genética
Fatores de Transcrição Maf Maior/metabolismo
Camundongos
Camundongos Endogâmicos C57BL
Fibras Musculares Esqueléticas/metabolismo
Ratos
Ratos Sprague-Dawley
Transativadores/genética
Transativadores/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Homeodomain Proteins); 0 (Maf Transcription Factors, Large); 0 (Trans-Activators); 0 (pancreatic and duodenal homeobox 1 protein)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170508
[Lr] Data última revisão:
170508
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170420
[St] Status:MEDLINE
[do] DOI:10.1186/s13287-017-0539-9


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[PMID]:28395471
[Au] Autor:Meng WW; Wang XH; Luo T; Li B; Wang QG; Guo R; Dai X; Zhang YJ; Abulikemu; Burenmingde; Azati; Abulimiti
[Ad] Endereço:The Center for Disease Control and Prevention of Xinjiang Uygur Autonomous Region, Urumqi 830002, China.
[Ti] Título:[Dynamics of F1 antibody responses to infection in ].
[So] Source:Zhonghua Yu Fang Yi Xue Za Zhi;51(4):353-357, 2017 Apr 06.
[Is] ISSN:0253-9624
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:To observe the dynamics of antibody response in great gerbils infected with in experiment. A total of 211 great gerbils were captured in the southern margin of plague natural focus of Junggar Basin of the Xinjiang Uygur Autonomous Region in 2011. Among them, there were 167 great gerbils without infection of and 44 great gerbils infected by . No. 2504 was employed for this experimental strain, which was strong toxic strain with negativity in the reduction experiment of nitrate. 35 great gerbils without the infection of were divided randomly and averagely into 7 groups including 6 experimental groups and 1 control group. Great gerbils in the 1st to 6th experimental groups were exposed first with 1 × 10(6)-1 × 10(11) CFU/ml of bacterial fluid with 10 times of gradient dilution; groin areas of great gerbils in the control group were injected subcutaneously with physiological saline; and the amount of infection was all 1 ml. 17 great gerbils infected with and the first detection of F1-antibody titer in 1∶256-1∶4 096 were grouped according to F1-antibody titer: group 1∶4 096 ( 4), group 1∶2 048 ( 4), group 1∶1 024 ( 3), group 1∶512 ( 3) and group 1∶256 ( 3); and blood in caudal regions was collected in asepsis for the detection of F1-antibody, with a total of 5 times. 9 great gerbils which were selected from the remaining great gerbils infected with and detected F1-antibody negative 2 times were exposed 1×10(6) CFU/ml of bacterial fluid for the second infection, with the amount of infection being 1 ml. Blood in caudal regions of great gerbils after the first and second infection were collected for the detection of plague F1-antibody on the 3rd, 5th, 7th, 15th, 30th, 60th, 90th and 120th day after infection. Declined regression models for great gerbils' antibodies were established with unary linear regression equation; declined change diagrams for the antibodies were drawn to observe the declined F1-antibody after great gerbils were exposed to . In great gerbils with the first infection of , antibodies were detected in the 1 × 10(6)-1 × 10(8) CFU/ml of group on the 30th, 15th and 15th day, respectively; the positive rates of antibody were 1/4, 3/4 and 4/5, respectively; the group 1×10(7) and 1× 10(8) CFU/ml reached to the highest antibody titer with 1∶256 on the 120th day; antibodies were revealed in the group 1×10(9), 1×10(10) and 1×10(11) CFU/ml from the 5th to 7th day when the seroconversion of all antibodies was observed; group 1×10(11) CFU/ml reached to the highest antibody titer on the 120th day with 1∶4 096. In the great gerbils with the second exposure to , positive antibodies were detected on the 3rd day with the positive rate being 2/9; and the highest antibody titer with 1∶2 048 was noted on the 90th day. Unary linear regression equation of declined F1 antibody of great gerbils was =0.045 0.321 ( 115.40, 0.001), and the shortest duration for F1-antibody titer declining from 1∶4 096 to 0 was 140 d and the longest duration 200 d. Great gerbils infected with the high concentration of fluid show shorter duration in producing F1-antibody, the antibody positive rate is also higher, and the highest antibody titer can reach 1∶4 096. The great gerbils could hold the plague F1 antibodies for a long time which was about 140 to 200 days from the highest titer.
[Mh] Termos MeSH primário: Anticorpos Antibacterianos/imunologia
Gerbillinae/imunologia
Peste/imunologia
Yersinia pestis/imunologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Antibacterianos/sangue
Formação de Anticorpos
China
Gerbillinae/microbiologia
Peste/sangue
Peste/microbiologia
Distribuição Aleatória
Fatores de Tempo
Yersinia pestis/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Bacterial)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170412
[St] Status:MEDLINE
[do] DOI:10.3760/cma.j.issn.0253-9624.2017.04.014



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