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  1 / 998 MEDLINE  
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[PMID]:29444087
[Au] Autor:Balsitis S; Gali V; Mason PJ; Chaniewski S; Levine SM; Wichroski MJ; Feulner M; Song Y; Granaldi K; Loy JK; Thompson CM; Lesniak JA; Brockus C; Kishnani N; Menne S; Cockett MI; Iyer R; Mason SW; Tenney DJ
[Ad] Endereço:Virology Discovery, Bristol-Myers Squibb, Wallingford, Connecticut, United States of America.
[Ti] Título:Safety and efficacy of anti-PD-L1 therapy in the woodchuck model of HBV infection.
[So] Source:PLoS One;13(2):e0190058, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Immune clearance of Hepatitis B virus (HBV) is characterized by broad and robust antiviral T cell responses, while virus-specific T cells in chronic hepatitis B (CHB) are rare and exhibit immune exhaustion that includes programmed-death-1 (PD-1) expression on virus-specific T cells. Thus, an immunotherapy able to expand and activate virus-specific T cells may have therapeutic benefit for CHB patients. Like HBV-infected patients, woodchucks infected with woodchuck hepatitis virus (WHV) can have increased hepatic expression of PD-1-ligand-1 (PD-L1), increased PD-1 on CD8+ T cells, and a limited number of virus-specific T cells with substantial individual variation in these parameters. We used woodchucks infected with WHV to assess the safety and efficacy of anti-PD-L1 monoclonal antibody therapy (αPD-L1) in a variety of WHV infection states. Experimentally-infected animals lacked PD-1 or PD-L1 upregulation compared to uninfected controls, and accordingly, αPD-L1 treatment in lab-infected animals had limited antiviral effects. In contrast, animals with naturally acquired WHV infections displayed elevated PD-1 and PD-L1. In these same animals, combination therapy with αPD-L1 and entecavir (ETV) improved control of viremia and antigenemia compared to ETV treatment alone, but with efficacy restricted to a minority of animals. Pre-treatment WHV surface antigen (sAg) level was identified as a statistically significant predictor of treatment response, while PD-1 expression on peripheral CD8+ T cells, T cell production of interferon gamma (IFN-γ) upon in vitro antigen stimulation (WHV ELISPOT), and circulating levels of liver enzymes were not. To further assess the safety of this strategy, αPD-L1 was tested in acute WHV infection to model the risk of liver damage when the extent of hepatic infection and antiviral immune responses were expected to be the greatest. No significant increase in serum markers of hepatic injury was observed over those in infected, untreated control animals. These data support a positive benefit/risk assessment for blockade of the PD-1:PD-L1 pathway in CHB patients and may help to identify patient groups most likely to benefit from treatment. Furthermore, the efficacy of αPD-L1 in only a minority of animals, as observed here, suggests that additional agents may be needed to achieve a more robust and consistent response leading to full sAg loss and durable responses through anti-sAg antibody seroconversion.
[Mh] Termos MeSH primário: Anticorpos Monoclonais/uso terapêutico
Antígeno B7-H1/imunologia
Modelos Animais de Doenças
Hepatite B/terapia
[Mh] Termos MeSH secundário: Animais
Anticorpos Monoclonais/efeitos adversos
Marmota
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Monoclonal); 0 (B7-H1 Antigen); 0 (CD274 protein, human)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180215
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190058


  2 / 998 MEDLINE  
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[PMID]:28809943
[Au] Autor:Yan J; Chen H; Lin G; Li Q; Chen J; Qin W; Su J; Zhang T
[Ad] Endereço:Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai, China.
[Ti] Título:Genetic evidence for subspecies differentiation of the Himalayan marmot, Marmota himalayana, in the Qinghai-Tibet Plateau.
[So] Source:PLoS One;12(8):e0183375, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The primary host of plague in the Qinghai-Tibet Plateau (QTP), China, is Marmota himalayana, which plays an essential role in the maintenance, transmission, and prevalence of plague. To achieve a more clear insight into the differentiation of M. himalayana, complete cytochrome b (cyt b) gene and 11 microsatellite loci were analyzed for a total of 423 individuals from 43 localities in the northeast of the QTP. Phylogenetic analyses with maximum likelihood and Bayesian inference methods showed that all derived haplotypes diverged into two primary well-supported monophyletic lineages, I and II, which corresponded to the referential sequences of two recognized subspecies, M. h. himalayana and M. h. robusta, respectively. The divergence between the two lineages was estimated to be at about 1.03 million years ago, nearly synchronously with the divergence between M. baibacina and M. kastschenkoi and much earlier than that between M. vancouverensis and M. caligata. Genetic structure analyses based on the microsatellite dataset detected significant admixture between the two lineages in the mixed region, which verified the intraspecies level of the differentiation between the two lineages. Our results for the first time demonstrated the coexistence of M. h. himalayana and M. h. robusta, and also, determined the distribution range of the two subspecies in the northeast of QTP. We provided fundamental information for more effective plague control in the QTP.
[Mh] Termos MeSH primário: Peste/transmissão
[Mh] Termos MeSH secundário: Animais
Teorema de Bayes
Marmota
Repetições de Microssatélites/genética
Filogenia
Peste/genética
Análise de Sequência de DNA
Tibet
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171011
[Lr] Data última revisão:
171011
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170816
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183375


  3 / 998 MEDLINE  
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[PMID]:28763517
[Au] Autor:Zanet S; Miglio G; Ferrari C; Bassano B; Ferroglio E; von Hardenberg A
[Ad] Endereço:Department of Veterinary Sciences, University of Turin, Grugliasco (To), Italy.
[Ti] Título:Higher risk of gastrointestinal parasite infection at lower elevation suggests possible constraints in the distributional niche of Alpine marmots.
[So] Source:PLoS One;12(8):e0182477, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Alpine marmots Marmota marmota occupy a narrow altitudinal niche within high elevation alpine environments. For animals living at such high elevations where resources are limited, parasitism represents a potential major cost in life history. Using occupancy models, we tested if marmots living at higher elevation have a reduced risk of being infected with gastro-intestinal helminths, possibly compensating the lower availability of resources (shorter feeding season, longer snow cover and lower temperature) than marmots inhabiting lower elevations. Detection probability of eggs and oncospheres of two gastro-intestinal helminthic parasites, Ascaris laevis and Ctenotaenia marmotae, sampled in marmot feces, was used as a proxy of parasite abundance. As predicted, the models showed a negative relationship between elevation and parasite detectability (i.e. abundance) for both species, while there appeared to be a negative effect of solar radiance only for C. marmotae. Site-occupancy models are used here for the first time to model the constrains of gastrointestinal parasitism on a wild species and the relationship existing between endoparasites and environmental factors in a population of free-living animals. The results of this study suggest the future use of site-occupancy models as a viable tool to account for parasite imperfect detection in eco-parasitological studies, and give useful insights to further investigate the hypothesis of the contribution of parasite infection in constraining the altitudinal niche of Alpine marmots.
[Mh] Termos MeSH primário: Altitude
Cestoides/isolamento & purificação
Enteropatias Parasitárias/diagnóstico
Enteropatias Parasitárias/veterinária
Marmota/parasitologia
[Mh] Termos MeSH secundário: Animais
Animais Selvagens/parasitologia
Doenças Transmissíveis
Itália
Probabilidade
Estações do Ano
Neve
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170802
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182477


  4 / 998 MEDLINE  
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[PMID]:28213271
[Au] Autor:Fu L; Hu H; Liu Y; Jing Z; Li W
[Ad] Endereço:Graduate School of Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China; National Institute of Biological Sciences, Beijing, China.
[Ti] Título:Woodchuck sodium taurocholate cotransporting polypeptide supports low-level hepatitis B and D virus entry.
[So] Source:Virology;505:1-11, 2017 May.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Sodium taurocholate cotransporting polypeptide (NTCP) is the functional receptor for human hepatitis B virus (HBV) and its satellite hepatitis D virus (HDV). Species barriers to HBV/HDV infection are mainly determined at entry level by variations in the sequences of particular NTCP orthologs. In this study, we sought to determine whether the NTCP ortholog in woodchuck (Marmota monax), woodchuck NTCP (wNTCP) supports viral infection. We found that wNTCP is capable of supporting HBV/HDV infection in HepG2 cells, but to much lower extent than human NTCP (hNTCP), which is about 90% reduction of hNTCP. Comprehensive site-directed mutagenesis mapping of hNTCP and wNTCP revealed that the residue at position 263 is a novel site crucial for viral entry. The important role of site 263 in infection is conserved among NTCP orthologs and may therefore be a potential target for blocking the viral entry.
[Mh] Termos MeSH primário: Vírus da Hepatite B/metabolismo
Vírus Delta da Hepatite/metabolismo
Especificidade de Hospedeiro/genética
Transportadores de Ânions Orgânicos Dependentes de Sódio/genética
Receptores Virais/genética
Simportadores/genética
Internalização do Vírus
[Mh] Termos MeSH secundário: Sequência de Aminoácidos/genética
Animais
Sítios de Ligação/genética
Linhagem Celular Tumoral
Clonagem Molecular
Células Hep G2
Hepatócitos/virologia
Seres Humanos
Fígado/metabolismo
Fígado/virologia
Masculino
Marmota/virologia
Transportadores de Ânions Orgânicos Dependentes de Sódio/metabolismo
Ligação Proteica/genética
Receptores Virais/metabolismo
Simportadores/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Organic Anion Transporters, Sodium-Dependent); 0 (Receptors, Virus); 0 (Symporters); 145420-23-1 (sodium-bile acid cotransporter)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170526
[Lr] Data última revisão:
170526
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170219
[St] Status:MEDLINE


  5 / 998 MEDLINE  
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[PMID]:28152393
[Au] Autor:Liu Y; Wang J; Wang L; Wang B; Yang S; Wang Q; Luo J; Feng X; Yang X; Lu Y; Roggendorf M; Lu M; Yang D; Liu J
[Ad] Endereço:Department of Infectious Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
[Ti] Título:Molecular cloning, characterization and expression analysis of Tim-3 and Galectin-9 in the woodchuck model.
[So] Source:Mol Immunol;83:127-136, 2017 Mar.
[Is] ISSN:1872-9142
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In recent years, a critical role for T cell immunoglobulin mucin domain 3 (Tim-3) and its ligand Galectin-9 (Gal-9) has emerged in infectious disease, autoimmunity and cancer. Manipulating this immune checkpoint may have immunotherapeutic potential and could represent an alternative approach for improving immune responses to viral infections and cancer. The woodchuck (Marmot monax) infected by woodchuck hepatitis virus (WHV) represents an informative animal model to study HBV infection and HCC. In the current study, the cDNA sequences of woodchuck Tim-3 and Gal-9 were cloned, sequenced and characterized. The extracellular domain of Tim-3 cDNA sequence consisted of 576bp coding sequence (CDS) that encoded 192 amino acids. The 1076bp full-length Gal-9 cDNA sequence consisted of 1059bp coding sequence (CDS) that encoded 352 amino acids with a molecular weight of 39.7kDa. The phylogenetic tree analysis revealed that the woodchuck Tim-3 and Gal-9 had the closest genetic relationship with Ictidomys tridecemlineatus. The result of quantification PCR analysis showed that ubiquitous expression of Gal-9 but not Tim-3 in different tissues of naive woodchucks. Elevated liver Gal-9 expression was observed in woodchucks with chronic WHV infection. Moreover, a polyclonal antibody against the extracellular domain of woodchuck Tim-3 were generated and identified by flow cytometry. Our results serve as a foundation for further insight into the role of Tim-3/Galectin-9 signaling pathway in viral hepatitis and HCC in the woodchuck model.
[Mh] Termos MeSH primário: Galectinas/genética
Receptor Celular 2 do Vírus da Hepatite A/genética
Marmota/genética
Marmota/imunologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Sequência de Bases
Clonagem Molecular
DNA Complementar/análise
DNA Complementar/genética
Galectinas/biossíntese
Infecções por Hepadnaviridae/genética
Infecções por Hepadnaviridae/imunologia
Receptor Celular 2 do Vírus da Hepatite A/biossíntese
Vírus da Hepatite B da Marmota
Hepatite Viral Animal/genética
Hepatite Viral Animal/imunologia
Filogenia
Reação em Cadeia da Polimerase
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Complementary); 0 (Galectins); 0 (Hepatitis A Virus Cellular Receptor 2)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170911
[Lr] Data última revisão:
170911
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170203
[St] Status:MEDLINE


  6 / 998 MEDLINE  
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[PMID]:28100306
[Au] Autor:Ao YY; Yu JM; Li LL; Cao JY; Deng HY; Xin YY; Liu MM; Lin L; Lu S; Xu JG; Duan ZJ
[Ad] Endereço:1​National Institute for Viral Disease Control and Prevention, China CDC, Beijing 100052, PR China.
[Ti] Título:Diverse novel astroviruses identified in wild Himalayan marmots.
[So] Source:J Gen Virol;98(4):612-623, 2017 Apr.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:With advances in viral surveillance and next-generation sequencing, highly diverse novel astroviruses (AstVs) and different animal hosts had been discovered in recent years. However, the existence of AstVs in marmots had yet to be shown. Here, we identified two highly divergent strains of AstVs (tentatively named Qinghai Himalayanmarmot AstVs, HHMAstV1 and HHMAstV2), by viral metagenomic analysis in liver tissues isolated from wild Marmota himalayana in China. Overall, 12 of 99 (12.1 %) M. himalayana faecal samples were positive for the presence of genetically diverse AstVs, while only HHMAstV1 and HHMAstV2 were identified in 300 liver samples. The complete genomic sequences of HHMAstV1 and HHMAstV2 were 6681 and 6610 nt in length, respectively, with the typical genomic organization of AstVs. Analysis of the complete ORF 2 sequence showed that these novel AstVs are most closely related to the rabbit AstV, mamastrovirus 23 (with 31.0 and 48.0 % shared amino acid identity, respectively). Phylogenetic analysis of the amino acid sequences of ORF1a, ORF1b and ORF2 indicated that HHMAstV1 and HHMAstV2 form two distinct clusters among the mamastroviruses, and may share a common ancestor with the rabbit-specific mamastrovirus 23. These results suggest that HHMAstV1 and HHMAstV2 are two novel species of the genus Mamastrovirus in the Astroviridae. The remarkable diversity of these novel AstVs will contribute to a greater understanding of the evolution and ecology of AstVs, although additional studies will be needed to understand the clinical significance of these novel AstVs in marmots, as well as in humans.
[Mh] Termos MeSH primário: Infecções por Astroviridae/veterinária
Astroviridae/classificação
Astroviridae/isolamento & purificação
Marmota/virologia
[Mh] Termos MeSH secundário: Animais
Astroviridae/genética
Infecções por Astroviridae/virologia
China
Análise por Conglomerados
Fezes/virologia
Ordem dos Genes
Genoma Viral
Fígado/virologia
Metagenômica
Filogenia
Análise de Sequência de DNA
Homologia de Sequência de Aminoácidos
Sintenia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170626
[Lr] Data última revisão:
170626
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170120
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000709


  7 / 998 MEDLINE  
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[PMID]:28062231
[Au] Autor:Petelle MB; Dang BN; Blumstein DT
[Ad] Endereço:Department of Ecology & Evolutionary Biology, University of California Los Angeles, CA, USA; Department of Zoology & Entomology, University of the Free State Qwaqwa, Phuthaditjhaba, South Africa. Electronic address: matthew.petelle@gmail.com.
[Ti] Título:The effect of maternal glucocorticoid levels on juvenile docility in yellow-bellied marmots.
[So] Source:Horm Behav;89:86-91, 2017 Mar.
[Is] ISSN:1095-6867
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Maternal effects can have significant and long-term consequences on offspring behavior and survival, while consistent individual differences (i.e., personality) can have profound impacts on individual fitness. Thus, both can influence population dynamics. However, the underlying mechanisms that determine variation in personality traits are poorly understood. Maternal effects are one potential mechanism that may explain personality variation. We capitalized on a long-term study of yellow-bellied marmots (Marmota flaviventris) to identify maternal effects on juvenile docility. To do so, we partitioned the variance in juvenile docility using a quantitative genetic modeling approach to isolate potential maternal effects. We also directly tested whether maternal stress, measured through fecal glucocorticoid metabolite levels during lactation of 82 mothers, was associated with offspring docility. Docility scores were estimated for 645 juveniles trapped between 2002 and 2012. We found an interaction between maternal glucocorticoid levels and dam age on juvenile docility. We also found significant maternal, litter, permanent environment, and year effects. These results suggest that a mother's life history stage interacts with stress to influence offspring personality. This early life influence can have long lasting effects on an individual's docility throughout life.
[Mh] Termos MeSH primário: Comportamento Animal/fisiologia
Glucocorticoides/fisiologia
Marmota/fisiologia
Mães
Personalidade/fisiologia
[Mh] Termos MeSH secundário: Animais
Feminino
Glucocorticoides/metabolismo
Masculino
Marmota/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glucocorticoids)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170904
[Lr] Data última revisão:
170904
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170108
[St] Status:MEDLINE


  8 / 998 MEDLINE  
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[PMID]:28056062
[Au] Autor:Suresh M; Korolowicz KE; Balarezo M; Iyer RP; Padmanabhan S; Cleary D; Gimi R; Sheri A; Yon C; Kallakury BV; Tucker RD; Afdhal N; Menne S
[Ad] Endereço:Department of Microbiology & Immunology, Georgetown University Medical Center, Washington, District of Columbia, United States of America.
[Ti] Título:Antiviral Efficacy and Host Immune Response Induction during Sequential Treatment with SB 9200 Followed by Entecavir in Woodchucks.
[So] Source:PLoS One;12(1):e0169631, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:SB 9200, an orally bioavailable dinucleotide, activates the viral sensor proteins, retinoic acid-inducible gene 1 (RIG-I) and nucleotide-binding oligomerization domain-containing protein 2 (NOD2) causing the induction of the interferon (IFN) signaling cascade for antiviral defense. The present study evaluated the overall antiviral response in woodchucks upon induction of immune response, first with SB 9200 followed by Entecavir (ETV) versus reduction of viral burden with ETV followed by SB 9200 immunomodulation. Woodchucks chronically infected with woodchuck hepatitis virus (WHV) were treated orally with SB 9200 (30 mg/kg/day) and ETV (0.5 mg/kg/day). Group 1 received ETV for 4 weeks followed by SB 9200 for 12 weeks. Group 2 received SB 9200 for 12 weeks followed by ETV for 4 weeks. At the end of treatment in Group 2, average reductions of 6.4 log10 in serum WHV DNA and 3.3 log10 in WHV surface antigen were observed whereas in Group 1, average reductions of 4.2 log10 and 1.1 log10 in viremia and antigenemia were noted. Both groups demonstrated marked reductions in hepatic WHV nucleic acid levels which were more pronounced in Group 2. Following treatment cessation and the 8-week follow-up, recrudescence of viral replication was observed in Group 1 while viral relapse in Group 2 was significantly delayed. The antiviral effects observed in both groups were associated with temporally different induction of IFN-α, IFN-ß, and IFN-stimulated genes in blood and liver. These results suggest that the induction of host immune responses by pretreatment with SB 9200 followed by ETV resulted in antiviral efficacy that was superior to that obtained using the strategy of viral reduction with ETV followed by immunomodulation.
[Mh] Termos MeSH primário: Antivirais/uso terapêutico
Guanina/análogos & derivados
Vírus da Hepatite B da Marmota/patogenicidade
Marmota/virologia
[Mh] Termos MeSH secundário: Animais
Guanina/uso terapêutico
Vírus da Hepatite B da Marmota/efeitos dos fármacos
Vírus da Hepatite B da Marmota/imunologia
Fígado/virologia
Replicação Viral/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antiviral Agents); 5968Y6H45M (entecavir); 5Z93L87A1R (Guanine)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170828
[Lr] Data última revisão:
170828
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170106
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0169631


  9 / 998 MEDLINE  
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[PMID]:27908555
[Au] Autor:Winer JN; Arzi B; Leale DM; Kass PH; Verstraete FJ
[Ad] Endereço:William R. Pritchard Veterinary Medical Teaching Hospital, School of Veterinary Medicine, University of California, Davis, CA, USA.
[Ti] Título:Dental Pathology of the Hoary Marmot (Marmota caligata), Groundhog (Marmota monax) and Alaska Marmot (Marmota broweri).
[So] Source:J Comp Pathol;156(1):42-52, 2017 Jan.
[Is] ISSN:1532-3129
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Museum specimens (maxillae and mandibles) of the three marmot species occurring in Alaska (Marmota caligata [n = 108 specimens], Marmota monax [n = 30] and Marmota broweri [n = 24]) were examined macroscopically according to predefined criteria. There were 71 specimens (43.8%) from female animals, 69 (42.6%) from male animals and 22 (13.6%) from animals of unknown sex. The ages of animals ranged from neonatal to adult, with 121 young adults (74.4%) and 41 adults (25.3%) included, and 168 excluded from study due to neonatal/juvenile age or incompleteness of specimens (missing part of the dentition). None of the teeth were missing, so 3,564 teeth were available for examination. All teeth were normal in morphology and none was affected by enamel hypoplasia. Two specimens displayed two supernumerary teeth each. One-third of specimens displayed attrition/abrasion and in 38.9% of these the change involved all premolar and molar teeth. The proportion of adult specimens affected by attrition/abrasion was three times as high as young adult specimens. Dental fractures were rare, noted in only two specimens, affecting 0.08% of teeth (n = 3). Periapical disease was also rare, with a striking lesion in one young adult female specimen. Some degree of periodontitis was seen in 26 specimens (16.1%), affecting 4.6% of premolar and molar teeth. The proportion of adults with periodontitis was over three times as high as that for young adults. Five specimens displayed mild malocclusion. Although the clinical significance of dental pathology in the marmot species of Alaska remains elusive, the occurrence and severity of some dental lesions may play an important role in their morbidity and mortality.
[Mh] Termos MeSH primário: Marmota
[Mh] Termos MeSH secundário: Alaska
Animais
Feminino
Masculino
Periodontite/epidemiologia
Dente/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170525
[Lr] Data última revisão:
170525
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161203
[St] Status:MEDLINE


  10 / 998 MEDLINE  
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[PMID]:27902227
[Au] Autor:Niu L; Lu S; Lai XH; Hu S; Chen C; Zhang G; Yang J; Jin D; Wang Y; Lan R; Lu G; Xie Y; Ye C; Xu J
[Ad] Endereço:1​School of Life Science, Shanxi University, Taiyuan 030006, PR China 2​Key Laboratory of Translation Medicine Tropical Diseases (Hainan Medical University), Ministry of Education, School of Tropical and Laboratory Medicine, Hainan Medical University, Haikoux, PR China 3​State Key Laboratory f
[Ti] Título:Streptococcus himalayensis sp. nov., isolated from the respiratory tract of Marmota himalayana.
[So] Source:Int J Syst Evol Microbiol;67(2):256-261, 2017 Feb.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Five strains of Gram-positive-staining, catalase-negative, coccus-shaped, chain-forming organisms isolated separately from the respiratory tracts of five Marmota himalayana animals in the Qinghai-Tibet Plateau of China were subjected to phenotypic and molecular taxonomic analyses. Comparative analysis of the 16S rRNA gene indicated that these singular organisms represent a new member of the genus Streptococcus, being phylogenetically closest to Streptococcus marmotae DSM 101995T (98.4 % similarity). The groEL, sodA and rpoB sequence analysis showed interspecies similarity values between HTS2T and Streptococcus. marmotae DSM 101995T, its closest phylogenetic relative based on 16S rRNA gene sequences, of 98.2, 78.8 and 93.7 %, respectively. A whole-genome phylogenetic tree built from 82 core genes of genomes from 16 species of the genus Streptococcus validated that HTS2T forms a distinct subline and exhibits specific phylogenetic affinity with S. marmotae. In silico DNA-DNA hybridization of HTS2T showed an estimated DNA reassociation value of 40.5 % with Streptococcus. marmotae DSM 101995T. On the basis of their phenotypic characteristics and phylogenetic findings, it is proposed that the five isolates be classified as representatives of a novel species of the genus Streptococcus, Streptococcus himalayensis sp. nov. The type strain is HTS2T (=DSM 101997T=CGMCC 1.15533T). The genome of Streptococcus himalayensis sp. nov. strain HTS2T contains 2195 genes with a size of 2 275 471 bp and a mean DNA G+C content of 41.3 mol%.
[Mh] Termos MeSH primário: Marmota/microbiologia
Filogenia
Sistema Respiratório/microbiologia
Streptococcus/classificação
[Mh] Termos MeSH secundário: Animais
Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Hibridização de Ácido Nucleico
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Streptococcus/genética
Streptococcus/isolamento & purificação
Tibet
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170818
[Lr] Data última revisão:
170818
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161201
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001609



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