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  1 / 415 MEDLINE  
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[PMID]:28809150
[Au] Autor:Engeland CE; Bossow S; Hudacek AW; Hoyler B; Förster J; Veinalde R; Jäger D; Cattaneo R; Ungerechts G; Springfeld C
[Ad] Endereço:1​Department of Medical Oncology, National Center for Tumor Diseases and University Hospital Heidelberg, Heidelberg, Germany 2​Department of Translational Oncology, National Center for Tumor Diseases and German Cancer Research Center, Heidelberg, Germany.
[Ti] Título:A Tupaia paramyxovirus vector system for targeting and transgene expression.
[So] Source:J Gen Virol;98(9):2248-2257, 2017 Sep.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Viruses from the diverse family of Paramyxoviridae include important pathogens and are applied in gene therapy and for cancer treatment. The Tupaia paramyxovirus (TPMV), isolated from the kidney of a tree shrew, does not infect human cells and neutralizing antibodies against other Paramyxoviridae do not cross-react with TPMV. Here, we present a vector system for de novo generation of infectious TPMV that allows for insertion of additional genes as well as targeting using antibody single-chain variable fragments. We show that the recombinant TPMV specifically infect cells expressing the targeted receptor and replicate in human cells. This vector system provides a valuable tool for both basic research and therapeutic applications.
[Mh] Termos MeSH primário: Técnicas de Transferência de Genes
Vetores Genéticos/genética
Paramyxoviridae/genética
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Vetores Genéticos/fisiologia
Seres Humanos
Paramyxoviridae/fisiologia
Transgenes
Tupaia/virologia
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170919
[Lr] Data última revisão:
170919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170816
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000887


  2 / 415 MEDLINE  
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[PMID]:28758632
[Au] Autor:Feng Y; Feng YM; Lu C; Han Y; Liu L; Sun X; Dai J; Xia X
[Ad] Endereço:1​Faculty of Life Science and Technology, Yunnan Provincial Center for Molecular Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500, PR China.
[Ti] Título:Tree shrew, a potential animal model for hepatitis C, supports the infection and replication of HCV in vitro and in vivo.
[So] Source:J Gen Virol;98(8):2069-2078, 2017 Aug.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The tree shrew (Tupaia belangeri chinensis), a small animal widely distributed in Southeast Asia and southwest China, has the potential to be developed as an animal model for hepatitis C. To determine the susceptibility of the tree shrew to hepatitis C virus (HCV) infection in vitro and in vivo, a well-established HCV, produced from the J6/JFH1-Huh7.5.1 culture system, was used to infect cultured primary tupaia hepatocytes (PTHs) and tree shrews. The in vitro results showed that HCV genomic RNA and HCV-specific nonstructural protein 5A (NS5A) could be detected in the PTH cell culture from days 3-15 post-infection, although the viral load was lower than that observed in Huh7.5.1 cell culture. The occurrence of five sense mutations [S391A, G397A, L402F and M405T in the hypervariable region 1 (HVR1) of envelope glycoprotein 2 and I2750M in NS5B] suggested that HCV undergoes genetic evolution during culture. Fourteen of the 30 experimental tree shrews (46.7 %) were found to be infected, although the HCV viremia was intermittent in vivo. A positive test for HCV RNA in liver tissue provided stronger evidence for HCV infection and replication in tree shrews. The results of an immunohistochemistry assay also demonstrated the presence of four HCV-specific proteins (Core, E2, NS3/4 and NS5A) in the hepatocytes of infected tree shrews. The pathological changes observed in the liver tissue of infected tree shrews could be considered to be representative symptoms of mild hepatitis. These results revealed that the tree shrew can be used as an animal model supporting the infection and replication of HCV in vitro and in vivo.
[Mh] Termos MeSH primário: Modelos Animais de Doenças
Hepacivirus/fisiologia
Hepatite C/virologia
Tupaia
Replicação Viral
[Mh] Termos MeSH secundário: Animais
Feminino
Hepacivirus/genética
Hepatite C/patologia
Seres Humanos
Fígado/patologia
Fígado/virologia
Masculino
Tupaia/virologia
Proteínas não Estruturais Virais/genética
Proteínas não Estruturais Virais/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (NS-5 protein, hepatitis C virus); 0 (Viral Nonstructural Proteins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171115
[Lr] Data última revisão:
171115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170801
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000869


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[PMID]:28427478
[Au] Autor:Uni S; Mat Udin AS; Agatsuma T; Saijuntha W; Junker K; Ramli R; Omar H; Lim YA; Sivanandam S; Lefoulon E; Martin C; Belabut DM; Kasim S; Abdullah Halim MR; Zainuri NA; Bhassu S; Fukuda M; Matsubayashi M; Harada M; Low VL; Chen CD; Suganuma N; Hashim R; Takaoka H; Azirun MS
[Ad] Endereço:Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, 50603, Malaysia. unishigehiko@um.edu.my.
[Ti] Título:Morphological and molecular characteristics of Malayfilaria sofiani Uni, Mat Udin & Takaoka n. g., n. sp. (Nematoda: Filarioidea) from the common treeshrew Tupaia glis Diard & Duvaucel (Mammalia: Scandentia) in Peninsular Malaysia.
[So] Source:Parasit Vectors;10(1):194, 2017 Apr 20.
[Is] ISSN:1756-3305
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The filarial nematodes Wuchereria bancrofti (Cobbold, 1877), Brugia malayi (Brug, 1927) and B. timori Partono, Purnomo, Dennis, Atmosoedjono, Oemijati & Cross, 1977 cause lymphatic diseases in humans in the tropics, while B. pahangi (Buckley & Edeson, 1956) infects carnivores and causes zoonotic diseases in humans in Malaysia. Wuchereria bancrofti, W. kalimantani Palmieri, Pulnomo, Dennis & Marwoto, 1980 and six out of ten Brugia spp. have been described from Australia, Southeast Asia, Sri Lanka and India. However, the origin and evolution of the species in the Wuchereria-Brugia clade remain unclear. While investigating the diversity of filarial parasites in Malaysia, we discovered an undescribed species in the common treeshrew Tupaia glis Diard & Duvaucel (Mammalia: Scandentia). METHODS: We examined 81 common treeshrews from 14 areas in nine states and the Federal Territory of Peninsular Malaysia for filarial parasites. Once any filariae that were found had been isolated, we examined their morphological characteristics and determined the partial sequences of their mitochondrial cytochrome c oxidase subunit 1 (cox1) and 12S rRNA genes. Polymerase chain reaction (PCR) products of the internal transcribed spacer 1 (ITS1) region were then cloned into the pGEM-T vector, and the recombinant plasmids were used as templates for sequencing. RESULTS: Malayfilaria sofiani Uni, Mat Udin & Takaoka, n. g., n. sp. is described based on the morphological characteristics of adults and microfilariae found in common treeshrews from Jeram Pasu, Kelantan, Malaysia. The Kimura 2-parameter distance between the cox1 gene sequences of the new species and W. bancrofti was 11.8%. Based on the three gene sequences, the new species forms a monophyletic clade with W. bancrofti and Brugia spp. The adult parasites were found in tissues surrounding the lymph nodes of the neck of common treeshrews. CONCLUSIONS: The newly described species appears most closely related to Wuchereria spp. and Brugia spp., but differs from these in several morphological characteristics. Molecular analyses based on the cox1 and 12S rRNA genes and the ITS1 region indicated that this species differs from both W. bancrofti and Brugia spp. at the genus level. We thus propose a new genus, Malayfilaria, along with the new species M. sofiani.
[Mh] Termos MeSH primário: Filariose/veterinária
Filarioidea/anatomia & histologia
Filarioidea/genética
Tupaia/parasitologia
[Mh] Termos MeSH secundário: Animais
Brugia/anatomia & histologia
Brugia/genética
DNA Espaçador Ribossômico/genética
Feminino
Filariose/epidemiologia
Filariose/parasitologia
Filarioidea/isolamento & purificação
Malásia
Masculino
Reação em Cadeia da Polimerase
Análise de Sequência de DNA
Wuchereria/anatomia & histologia
Wuchereria/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Ribosomal Spacer)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170422
[St] Status:MEDLINE
[do] DOI:10.1186/s13071-017-2105-9


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[PMID]:28384717
[Au] Autor:Liu HH; Kenning MS; Jobling AI; McBrien NA; Gentle A
[Ad] Endereço:Department of Optometry and Vision Sciences, The University of Melbourne, Melbourne, Victoria, Australia 2School of Optometry and Vision Science, University of California Berkley, Berkley, California, United States.
[Ti] Título:Reduced Scleral TIMP-2 Expression Is Associated With Myopia Development: TIMP-2 Supplementation Stabilizes Scleral Biomarkers of Myopia and Limits Myopia Development.
[So] Source:Invest Ophthalmol Vis Sci;58(4):1971-1981, 2017 Apr 01.
[Is] ISSN:1552-5783
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Purpose: The purpose of this study was to determine the endogenous regulation pattern of tissue inhibitor of metalloproteinase-2 (TIMP-2) in the tree shrew sclera during myopia development and investigate the capacity of exogenous TIMP-2 to inhibit matrix metalloproteinase-2 (MMP-2) in vitro and both scleral collagen degradation and myopia development in vivo. Methods: TIMP-2 expression in the sclera during myopia development was assessed using polymerase chain reaction. In vitro TIMP-2 inhibition of MMP-2 was investigated using a gelatinase activity plate assay and zymography. Tree shrews were injected with a collagen precursor before undergoing monocular form deprivation and concurrent daily subconjunctival injections of either TIMP-2 or vehicle to the form-deprived eye. In vivo ocular biometry changes were monitored, and scleral tissue was collected after 12 days and assayed for collagen degradation. Results: The development of myopia was associated with a mean reduction in TIMP-2 mRNA expression after 5 days of form deprivation (P < 0.01). Both activation and activity of MMP-2 were inhibited by TIMP-2 with an IC50 of 10 to 20 and 2 nM, respectively. In vivo exogenous addition of TIMP-2 significantly reduced myopia development (P < 0.01), due to reduced vitreous chamber elongation (P < 0.01). In vivo TIMP-2 treatment also significantly inhibited posterior scleral collagen degradation relative to vehicle-treated eyes (P < 0.01), with levels similar to those in control eyes. Conclusions: Myopia development in mammals is associated with reduced expression of TIMP-2, which contributes to increased degradative activity in the sclera. It follows that replenishment of this TIMP-2 significantly reduced the rate of both scleral collagen degradation and myopia development.
[Mh] Termos MeSH primário: Regulação da Expressão Gênica no Desenvolvimento
Miopia/genética
RNA/genética
Esclera/enzimologia
Inibidor Tecidual de Metaloproteinase-2/genética
Inibidor Tecidual de Metaloproteinase-2/uso terapêutico
[Mh] Termos MeSH secundário: Animais
Animais Recém-Nascidos
Biomarcadores/metabolismo
Biometria
Células Cultivadas
Colágeno/metabolismo
Modelos Animais de Doenças
Feminino
Fibroblastos/metabolismo
Fibroblastos/patologia
Inibidores de Metaloproteinases de Matriz/uso terapêutico
Miopia/tratamento farmacológico
Miopia/metabolismo
RNA Mensageiro
Reação em Cadeia da Polimerase em Tempo Real
Inibidor Tecidual de Metaloproteinase-2/biossíntese
Tupaia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Matrix Metalloproteinase Inhibitors); 0 (RNA, Messenger); 127497-59-0 (Tissue Inhibitor of Metalloproteinase-2); 63231-63-0 (RNA); 9007-34-5 (Collagen)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170626
[Lr] Data última revisão:
170626
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170407
[St] Status:MEDLINE
[do] DOI:10.1167/iovs.16-21181


  5 / 415 MEDLINE  
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[PMID]:28302963
[Au] Autor:Tang DH; Ye YS; Wang CY; Li ZL; Zheng H; Ma KL
[Ad] Endereço:Medical Primate Research Center of China, Institute of Medical Biology, Chinese Academy of Medical Sciences/Peking Union Medical College, No. 935, Jiaoling Road, Kunming, Yunnan 650118, P.R. China.
[Ti] Título:Potassium oxonate induces acute hyperuricemia in the tree shrew (tupaia belangeri chinensis).
[So] Source:Exp Anim;66(3):209-216, 2017 Aug 05.
[Is] ISSN:1881-7122
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Potassium oxonate, a selectively competitive uricase inhibitor, produced hyperuricemia (HUA) in rodents in a previous study. In this study, we employed the tree shrew as an animal model to study potassium oxonate-induced HUA. The effect of allopurinol (ALLO), a uric acid reducer, was also examined in this model. Potassium oxonate at doses of 5, 20, 40, 60, 80, 100, and 1,000 mg/kg was given intraperitoneally to tree shrews. The results showed that potassium oxonate can effectively increase the levels of uric acid in tree shrews at doses ranging from 40 to 100 mg/kg. Semiquantitative RT-PCR showed that the xanthine dehydrogenase/oxidase (XDH/XO) mRNA expression level was significantly higher in the liver tissue of tree shrews with high levels of uric acid. There were no changes in serum urea nitrogen, or serum creatinine values. ALLO can significantly decrease serum uric acid levels (P<0.01) and raise XDH/XO mRNA expression levels in the liver tissue of tree shrews with HUA. XDH/XO mRNA expression levels did not change in untreated tree shrews. Studies on acute toxicity in the tree shrew did not show any significantly abnormal signs. There were no adverse effects at the macroscopic level up to doses ≤100 mg/kg. Potassium oxonate induced acute HUA in tree shrews at lower doses compared with other animal models. Potassium oxonate-treated tree shrews may be a potential animal model for studying pathogenic mechanism and evaluating a new therapeutic agent for treatment of HUA in humans.
[Mh] Termos MeSH primário: Modelos Animais de Doenças
Inibidores Enzimáticos/efeitos adversos
Hiperuricemia/induzido quimicamente
Ácido Oxônico/efeitos adversos
Tupaia
[Mh] Termos MeSH secundário: Doença Aguda
Alopurinol/farmacologia
Alopurinol/uso terapêutico
Animais
Relação Dose-Resposta a Droga
Inibidores Enzimáticos/administração & dosagem
Expressão Gênica
Seres Humanos
Hiperuricemia/tratamento farmacológico
Injeções Intraperitoneais
Fígado/metabolismo
Ácido Oxônico/administração & dosagem
RNA Mensageiro/metabolismo
Urato Oxidase/antagonistas & inibidores
Ácido Úrico/metabolismo
Xantina Desidrogenase/genética
Xantina Desidrogenase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Enzyme Inhibitors); 0 (RNA, Messenger); 268B43MJ25 (Uric Acid); 4R7FFA00RX (potassium oxonate); 5VT6420TIG (Oxonic Acid); 63CZ7GJN5I (Allopurinol); EC 1.17.1.4 (Xanthine Dehydrogenase); EC 1.7.3.3 (Urate Oxidase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170928
[Lr] Data última revisão:
170928
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170318
[St] Status:MEDLINE
[do] DOI:10.1538/expanim.16-0096


  6 / 415 MEDLINE  
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[PMID]:27171107
[Au] Autor:Bai W; Cui X; Chen R; Tao S; Hong R; Zhang J; Zhang J; Wang Y; Xie Y; Liu J
[Ad] Endereço:Key Laboratory of Medical Molecular Virology (MOH & MOE) and Institutes of Biomedical Sciences, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032, China. bwy.1989@163.com.
[Ti] Título:Re-Designed Recombinant Hepatitis B Virus Vectors Enable Efficient Delivery of Versatile Cargo Genes to Hepatocytes with Improved Safety.
[So] Source:Viruses;8(5), 2016 May 10.
[Is] ISSN:1999-4915
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Hepatitis B virus (HBV) takes humans as its sole natural host, and productive infection in vivo is restricted exclusively to hepatocytes in the liver. Consequently, HBV-derived viral vectors are attractive candidates for liver-targeting gene therapies. Previously, we developed a novel recombinant HBV vector, designated 5c3c, from a highly replicative clinical isolate. 5c3c was demonstrated to be capable of efficiently delivering protein or RNA expression into infected primary tupaia hepatocytes (PTH), but the design of 5c3c imposes stringent restrictions on inserted sequences, which have limited its wider adoption. In this work, we addressed issues with 5c3c by re-designing the insertion strategy. The resultant vector, designated 5dCG, was more replicative than parental 5c3c, imposed no specific restrictions on inserted sequences, and allowed insertion of a variety of cargo genes without significant loss of replication efficiency. 5dCG-based recombinant HBV effectively delivered protein and RNA expression into infected PTH. Furthermore, due to the loss of functional core ORF, 5dCG vectors depend on co-infecting wild type HBV for replication and efficient expression of cargo genes. Development of the improved 5dCG vector makes wider applications of recombinant HBV possible, while dependence on co-infecting wild type HBV results in improved safety for certain in vivo applications.
[Mh] Termos MeSH primário: Vetores Genéticos
Vírus da Hepatite B/genética
Hepatócitos/virologia
Transdução Genética
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Tupaia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170913
[Lr] Data última revisão:
170913
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160513
[St] Status:MEDLINE


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[PMID]:27041457
[Au] Autor:Hai-Ying C; Nagano K; Ezzikouri S; Yamaguchi C; Kayesh ME; Rebbani K; Kitab B; Nakano H; Kouji H; Kohara M; Tsukiyama-Kohara K
[Ad] Endereço:Transboundary Animal Diseases Center, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima-city, Kagoshima 890-0065, Japan.
[Ti] Título:Establishment of an intermittent cold stress model using Tupaia belangeri and evaluation of compound C737 targeting neuron-restrictive silencer factor.
[So] Source:Exp Anim;65(3):285-92, 2016 Jul 29.
[Is] ISSN:1881-7122
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Previous studies have shown that intermittent cold stress (ICS) induces depression-like behaviors in mammals. Tupaia belangeri (the tree shrew) is the only experimental animal other than the chimpanzee that has been shown to be susceptible to infection by hepatitis B and C viruses. Moreover, full genome sequence analysis has revealed strong homology between host proteins in Tupaia and in humans and other primates. Tupaia neuromodulator receptor proteins are also known to have a high degree of homology with their corresponding primate proteins. Based on these similarities, we hypothesized that induction of ICS in Tupaia would provide a useful animal model of stress responses. We exposed young adult Tupaia to ICS and observed decreases in body temperature and body weight in both female and male Tupaia, suggesting that Tupaia are an appropriate animal model for ICS studies. We further examined the efficacy of a new small-molecule compound, C737, against the effects of ICS. C737 mimics the helical structure of neuron-restrictive silencer factor (NRSF/REST), which regulates a wide range of target genes involved in neuronal function and pain modulation. Treatment with C737 significantly reduced stress-induced weight loss in female Tupaia; these effects were stronger than those elicited by the antidepressant agomelatine. These results suggest that Tupaia represents a useful non-rodent ICS model. Our data also provide new insights into the function of NRSF/REST in stress-induced depression and other disorders with epigenetic influences or those with high prevalence in women.
[Mh] Termos MeSH primário: Resposta ao Choque Frio/efeitos dos fármacos
Resposta ao Choque Frio/genética
Modelos Animais
Proteínas Repressoras/fisiologia
Tupaia
[Mh] Termos MeSH secundário: Acetamidas/farmacologia
Animais
Antidepressivos/farmacologia
Resposta ao Choque Frio/fisiologia
Depressão/etiologia
Depressão/genética
Epigênese Genética
Feminino
Seres Humanos
Masculino
Neurônios/fisiologia
Dor/etiologia
Dor/genética
Perda de Peso/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetamides); 0 (Antidepressive Agents); 0 (RE1-silencing transcription factor); 0 (Repressor Proteins); 138112-76-2 (S 20098)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170313
[Lr] Data última revisão:
170313
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160405
[St] Status:MEDLINE
[do] DOI:10.1538/expanim.15-0123


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[PMID]:26880187
[Au] Autor:Yu W; Yang C; Bi Y; Long F; Li Y; Wang J; Huang F
[Ad] Endereço:Medical Faculty, Kunming University of Science and Technology, Kunming, China. wenhaiyu1234@163.com.
[Ti] Título:Characterization of hepatitis E virus infection in tree shrew (Tupaia belangeri chinensis).
[So] Source:BMC Infect Dis;16:80, 2016 Feb 16.
[Is] ISSN:1471-2334
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Hepatitis E virus (HEV) is a major cause of hepatitis in developing countries and poses a threat to public health worldwide. Tree shrew (Tupaia belangeri chinensis) is a useful animal model in studies on hepatitis viruses, such as hepatitis B and C viruses. However, the use of this animal model for HEV research is yet to be developed. METHODS: Tree shrews were intravenously (IV) injected with swine genotype 4 HEV or infected by contact-exposure to IV infected tree shrews. RT-nPCR was performed to detect HEV RNA in the feces, tissues, and blood. HEV capsid protein in the different tissues was detected by Western blot and estimated by quantitative RT-PCR. Anti-HEV antibodies were determined by ELISA. Liver damages were evaluated by histopathologic examination and analysis of liver-specific enzymes activities. RESULTS: Both negative and positive strands of HEV RNA were detected in the feces of the HEV-infected or contact-exposed tree shrews 3-4 days post-inoculation. HEV RNA was detectable in the liver, spleen, kidneys, and bile. Virusemia developed in all the HEV-infected tree shrews. HEV capsid protein was expressed in the liver, spleen, and kidneys. The histological examination and analysis of liver-specific enzymes activities showed that HEV caused acute liver lesions in the tree shrews. Meanwhile, the infected tree shrews showed positive IgG and IgM antibodies. CONCLUSIONS: Tree shrews are susceptible to HEV and may be useful animal models for HEV experimental infection studies on pathogenesis or preclinical drug development.
[Mh] Termos MeSH primário: Modelos Animais de Doenças
Hepatite E
Tupaia
[Mh] Termos MeSH secundário: Animais
Anticorpos Anti-Hepatite
Hepatite E/virologia
Vírus da Hepatite E/isolamento & purificação
Seres Humanos
Masculino
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Hepatitis Antibodies)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160217
[St] Status:MEDLINE
[do] DOI:10.1186/s12879-016-1418-1


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[PMID]:26843607
[Au] Autor:Poirot J; De Luna P; Rainer G
[Ad] Endereço:Visual Cognition Laboratory, Department of Medicine, University of Fribourg, Fribourg, Switzerland.
[Ti] Título:Neural coding of image structure and contrast polarity of Cartesian, hyperbolic, and polar gratings in the primary and secondary visual cortex of the tree shrew.
[So] Source:J Neurophysiol;115(4):2000-13, 2016 Apr.
[Is] ISSN:1522-1598
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We comprehensively characterize spiking and visual evoked potential (VEP) activity in tree shrew V1 and V2 using Cartesian, hyperbolic, and polar gratings. Neural selectivity to structure of Cartesian gratings was higher than other grating classes in both visual areas. From V1 to V2, structure selectivity of spiking activity increased, whereas corresponding VEP values tended to decrease, suggesting that single-neuron coding of Cartesian grating attributes improved while the cortical columnar organization of these neurons became less precise from V1 to V2. We observed that neurons in V2 generally exhibited similar selectivity for polar and Cartesian gratings, suggesting that structure of polar-like stimuli might be encoded as early as in V2. This hypothesis is supported by the preference shift from V1 to V2 toward polar gratings of higher spatial frequency, consistent with the notion that V2 neurons encode visual scene borders and contours. Neural sensitivity to modulations of polarity of hyperbolic gratings was highest among all grating classes and closely related to the visual receptive field (RF) organization of ON- and OFF-dominated subregions. We show that spatial RF reconstructions depend strongly on grating class, suggesting that intracortical contributions to RF structure are strongest for Cartesian and polar gratings. Hyperbolic gratings tend to recruit least cortical elaboration such that the RF maps are similar to those generated by sparse noise, which most closely approximate feedforward inputs. Our findings complement previous literature in primates, rodents, and carnivores and highlight novel aspects of shape representation and coding occurring in mammalian early visual cortex.
[Mh] Termos MeSH primário: Sensibilidades de Contraste
Potenciais Evocados Visuais
Córtex Visual/fisiologia
[Mh] Termos MeSH secundário: Animais
Neurônios/fisiologia
Tupaia
Córtex Visual/citologia
Campos Visuais
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1612
[Cu] Atualização por classe:170403
[Lr] Data última revisão:
170403
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160205
[St] Status:MEDLINE
[do] DOI:10.1152/jn.01000.2015


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[PMID]:26654952
[Au] Autor:Sanada T; Tsukiyama-Kohara K; Yamamoto N; Ezzikouri S; Benjelloun S; Murakami S; Tanaka Y; Tateno C; Kohara M
[Ad] Endereço:Department of Microbiology and Cell Biology, Tokyo Metropolitan Institute of Medical Science, 2-1-6, Kamikitazawa, Setagaya-ku, Tokyo 156-8506, Japan.
[Ti] Título:Property of hepatitis B virus replication in Tupaia belangeri hepatocytes.
[So] Source:Biochem Biophys Res Commun;469(2):229-35, 2016 Jan 08.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The northern treeshrew (Tupaia belangeri) has been reported to be an effective candidate for animal infection model with hepatitis B virus (HBV). The objective of our study was to analyze the growth characteristics of HBV in tupaia hepatocytes and the host response to HBV infection. We established primary tupaia hepatocytes (3-6-week old tupaia) and infected them with HBV genotypes A, B and C, and all the genotypes proliferated as well as those in human primary hepatocytes (>10(5) copies/ml in culture supernatant). We next generated a chimeric mouse with tupaia liver by transplantation of tupaia primary hepatocytes to urokinase-type plasminogen activator cDNA (cDNA-uPA)/severe combined immunodeficient (SCID) mice and the replacement ratio with tupaia hepatocytes was found to be more than 95%. Infection of chimeric mice with HBV (genotypes B, C, and D) resulted in HBV-DNA level of 10(4)-10(6) copies/ml after 8 weeks of infection, which were almost similar to that in humanized chimeric mouse. In contrast, serum HBV level in adult tupaia (1-year-old tupaia) was quite low (<10(3) copies/ml). Understanding the differences in the response to HBV infection in primary tupaia hepatocytes, chimeric mouse, and adult tupaia will contribute to elucidating the mechanism of persistent HBV infection and viral eradication. Thus, T. belangeri was found to be efficient for studying the host response to HBV infection, thereby providing novel insight into the pathogenesis of HBV.
[Mh] Termos MeSH primário: Vírus da Hepatite B/fisiologia
Hepatite B/virologia
Hepatócitos/virologia
Tupaia/virologia
Replicação Viral/fisiologia
[Mh] Termos MeSH secundário: Animais
Camundongos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1605
[Cu] Atualização por classe:160101
[Lr] Data última revisão:
160101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151215
[St] Status:MEDLINE



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