Base de dados : MEDLINE
Pesquisa : B01.050.150.900.649.573.575 [Categoria DeCS]
Referências encontradas : 4043 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 405 ir para página                         

  1 / 4043 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
[PMID]:29211106
[Au] Autor:Vandermark C; Zieman E; Boyles E; Nielsen CK; Davis C; Jiménez FA
[Ad] Endereço:Southern Illinois University, Department of Zoology, Carbondale, IL, USA.
[Ti] Título:Trypanosoma cruzi strain TcIV infects raccoons from Illinois.
[So] Source:Mem Inst Oswaldo Cruz;113(1):30-37, 2018 Jan.
[Is] ISSN:1678-8060
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The northern limits of Trypanosoma cruzi across the territory of the United States remain unknown. The known vectors Triatoma sanguisuga and T. lecticularia find their northernmost limits in Illinois; yet, earlier screenings of those insects did not reveal the presence of the pathogen, which has not been reported in vectors or reservoir hosts in this state. OBJECTIVES: Five species of medium-sized mammals were screened for the presence of T. cruzi. METHODS: Genomic DNA was isolated from heart, spleen and skeletal muscle of bobcats (Lynx rufus, n = 60), raccoons (Procyon lotor, n = 37), nine-banded armadillos (Dasypus novemcinctus, n = 5), Virginia opossums (Didelphis virginiana, n = 3), and a red fox (Vulpes vulpes). Infections were detected targeting DNA from the kinetoplast DNA minicircle (kDNA) and satellite DNA (satDNA). The discrete typing unit (DTU) was determined by amplifying two gene regions: the Spliced Leader Intergenic Region (SL), via a multiplex polymerase chain reaction, and the 24Sα ribosomal DNA via a heminested reaction. Resulting sequences were used to calculate their genetic distance against reference DTUs. FINDINGS: 18.9% of raccoons were positive for strain TcIV; the rest of mammals tested negative. MAIN CONCLUSIONS: These results confirm for the first time the presence of T. cruzi in wildlife from Illinois, suggesting that a sylvatic life cycle is likely to occur in the region. The analyses of sequences of SL suggest that amplicons resulting from a commonly used multiplex reaction may yield non-homologous fragments.
[Mh] Termos MeSH primário: Tatus/parasitologia
Doença de Chagas/veterinária
Raposas/parasitologia
Lynx/parasitologia
Gambás/parasitologia
Guaxinins/parasitologia
Trypanosoma cruzi/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Doença de Chagas/diagnóstico
Illinois
Trypanosoma cruzi/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180131
[Lr] Data última revisão:
180131
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171207
[St] Status:MEDLINE


  2 / 4043 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29068939
[Au] Autor:Israel JS; McCarthy JE; Rose KR; Rao VK
[Ad] Endereço:Madison, Wis.; and Fort Collins, Colo. From the Division of Plastic Surgery, University of Wisconsin, Madison; and Northern Colorado Plastic and Hand Surgery.
[Ti] Título:Watch Out for Wild Animals: A Systematic Review of Upper Extremity Injuries Caused by Uncommon Species.
[So] Source:Plast Reconstr Surg;140(5):1008-1022, 2017 Nov.
[Is] ISSN:1529-4242
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Across the world, many species of nondomesticated animals dwell among humans in metropolitan areas. Rare animal bites pose a dilemma for hand surgeons, as they often result in operative injuries and recalcitrant infections. The authors treated an 85-year-old man who experienced severe cellulitis of the index finger following an opossum bite. This case prompted a systematic review of upper extremity injuries caused by species other than dogs, cats, snakes, and insects. METHODS: The authors conducted a systematic review of PubMed and Scopus databases to identify relevant articles published between 1980 and 2016. Two reviewers critically appraised the studies that met inclusion and exclusion criteria. RESULTS: The hand infection in the man who sustained an opossum bite at the authors' institution was successfully treated with targeted antibiotic therapy, hand elevation, and splinting. Seventy-one articles met inclusion criteria for and were included in this systematic review. The vast majority of existing articles represent level IV and level V evidence. The relevant literature suggests that the majority of hand infections attributable to animal bites and stings are polymicrobial. CONCLUSIONS: Injuries secondary to aquatic animals appear to be the most frequently described in the literature, and hot water immersion should be used for the majority of envenomation attributable to aquatic species. Infections can often be treated with an aminopenicillin antibiotic combined with a beta-lactamase inhibitor. Given the variability in presentation and potential for sequelae such as soft-tissue necrosis and systemic reactions, hand surgeons should approach such upper extremity injuries with a high degree of caution.
[Mh] Termos MeSH primário: Animais Selvagens
Mordeduras e Picadas
Extremidade Superior/lesões
[Mh] Termos MeSH secundário: Idoso de 80 Anos ou mais
Animais
Antibacterianos/uso terapêutico
Mordeduras e Picadas/diagnóstico
Mordeduras e Picadas/etiologia
Mordeduras e Picadas/terapia
Terapia Combinada
Traumatismos da Mão/diagnóstico
Traumatismos da Mão/etiologia
Traumatismos da Mão/terapia
Seres Humanos
Masculino
Gambás
Procedimentos Ortopédicos
Infecção dos Ferimentos
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Anti-Bacterial Agents)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:171026
[St] Status:MEDLINE
[do] DOI:10.1097/PRS.0000000000003754


  3 / 4043 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28759578
[Au] Autor:Vieira SM; da Rocha SLG; Neves-Ferreira AGDC; Almeida RV; Perales J
[Ad] Endereço:Laboratory of Toxinology, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, RJ, Brazil.
[Ti] Título:Heterologous expression of the antimyotoxic protein DM64 in Pichia pastoris.
[So] Source:PLoS Negl Trop Dis;11(7):e0005829, 2017 Jul.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Snakebite envenomation is a neglected condition that constitutes a public health problem in tropical and subtropical countries, including Brazil. Interestingly, some animals are resistant to snake envenomation due to the presence of inhibitory glycoproteins in their serum that target toxic venom components. DM64 is an acidic glycoprotein isolated from Didelphis aurita (opossum) serum that has been characterized as an inhibitor of the myotoxicity induced by bothropic toxins bearing phospholipase A2 (PLA2) structures. This antitoxic protein can serve as an excellent starting template for the design of novel therapeutics against snakebite envenomation, particularly venom-induced local tissue damage. Therefore, the aim of this work was to produce a recombinant DM64 (rDM64) in the methylotrophic yeast Pichia pastoris and to compare its biological properties with those of native DM64. Yeast fermentation in the presence of Pefabloc, a serine protease inhibitor, stimulated cell growth (~1.5-fold), increased the rDM64 production yield approximately 10-fold and significantly reduced the susceptibility of rDM64 to proteolytic degradation. P. pastoris fermentation products were identified by mass spectrometry and Western blotting. The heterologous protein was efficiently purified from the culture medium by affinity chromatography (with immobilized PLA2 myotoxin) and/or an ion exchange column. Although both native and recombinant DM64 exhibit different glycosylation patterns, they show very similar electrophoretic mobilities after PNGase F treatment. rDM64 formed a noncovalent complex with myotoxin II (Lys49-PLA2) from Bothrops asper and displayed biological activity that was similar to that of native DM64, inhibiting the cytotoxicity of myotoxin II by 92% at a 1:1 molar ratio.
[Mh] Termos MeSH primário: Proteínas Sanguíneas/química
Inibidores de Fosfolipase A2/química
Fosfolipases A2/química
Proteínas de Répteis/química
Venenos de Serpentes/química
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Proteínas Sanguíneas/biossíntese
Bothrops
Brasil
Linhagem Celular
Espectrometria de Massas
Camundongos
Gambás
Pichia
Proteínas Recombinantes/biossíntese
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Blood Proteins); 0 (DM64 protein, Didelphis marsupialis); 0 (Phospholipase A2 Inhibitors); 0 (Recombinant Proteins); 0 (Reptilian Proteins); 0 (Snake Venoms); EC 3.1.1.4 (Phospholipases A2)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170827
[Lr] Data última revisão:
170827
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170801
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005829


  4 / 4043 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28665952
[Au] Autor:Lovera R; Fernández MS; Jacob J; Lucero N; Morici G; Brihuega B; Farace MI; Caracostantogolo J; Cavia R
[Ad] Endereço:Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires and Instituto de Ecología, Genética y Evolución de Buenos Aires (IEGEBA), UBA-CONICET, Cdad. Autónoma de Buenos Aires, Argentina.
[Ti] Título:Intrinsic and extrinsic factors related to pathogen infection in wild small mammals in intensive milk cattle and swine production systems.
[So] Source:PLoS Negl Trop Dis;11(6):e0005722, 2017 Jun.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Understanding the ecological processes that are involved in the transmission of zoonotic pathogens by small mammals may aid adequate and effective management measures. Few attempts have been made to analyze the ecological aspects that influence pathogen infection in small mammals in livestock production systems. We describe the infection of small mammals with Leptospira spp., Brucella spp., Trichinella spp. and Cysticercus fasciolaris and assess the related intrinsic and extrinsic factors in livestock production systems in central Argentina at the small mammal community, population and individual levels. METHODOLOGY/PRINCIPAL FINDINGS: Ten pig farms and eight dairy farms were studied by removal trapping of small mammals from 2008 to 2011. Each farm was sampled seasonally over the course of one year with cage and Sherman live traps. The 505 small mammals captured (14,359 trap-nights) included three introduced murine rodents, four native rodents and two opossums. Leptospira spp., anti-Brucella spp. antibodies and Trichinella spp. were found in the three murine rodents and both opossums. Rattus norvegicus was also infected with C. fasciolaris; Akodon azarae and Oligoryzomys flavescens with Leptospira spp.; anti-Brucella spp. antibodies were found in A. azarae. Two or more pathogens occurred simultaneously on 89% of the farms, and each pathogen was found on at least 50% of the farms. Pathogen infections increased with host abundance. Infection by Leptospira spp. also increased with precipitation and during warm seasons. The occurrence of anti-Brucella spp. antibodies was higher on dairy farms and during the winter and summer. The host abundances limit values, from which farms are expected to be free of the studied pathogens, are reported. CONCLUSIONS/SIGNIFICANCE: Murine rodents maintain pathogens within farms, whereas other native species are likely dispersing pathogens among farms. Hence, we recommend preventing and controlling murines in farm dwellings and isolating farms from their surroundings to avoid contact with other wild mammals.
[Mh] Termos MeSH primário: Infecções Bacterianas/veterinária
Gambás/microbiologia
Gambás/parasitologia
Doenças Parasitárias em Animais/epidemiologia
Roedores/microbiologia
Roedores/parasitologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Antibacterianos/sangue
Argentina
Infecções Bacterianas/epidemiologia
Brucella/imunologia
Bovinos
Fazendas
Leptospira/isolamento & purificação
Prevalência
Suínos
Taenia/isolamento & purificação
Trichinella/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Bacterial)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170808
[Lr] Data última revisão:
170808
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170701
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005722


  5 / 4043 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28615248
[Au] Autor:Eshbach ML; Sethi R; Avula R; Lamb J; Hollingshead DJ; Finegold DN; Locker JD; Chandran UR; Weisz OA
[Ad] Endereço:Renal-Electrolyte Division, Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania.
[Ti] Título:The transcriptome of the opossum kidney OK proximal tubule cell line.
[So] Source:Am J Physiol Renal Physiol;313(3):F585-F595, 2017 Sep 01.
[Is] ISSN:1522-1466
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The OK cell line derived from the kidney of a female opossum has proven to be a useful model in which to investigate the unique regulation of ion transport and membrane trafficking mechanisms in the proximal tubule (PT). Sequence data and comparison of the transcriptome of this cell line to eutherian mammal PTs would further broaden the utility of this culture model. However, the genomic sequence for is not available and although a draft genome sequence for the opossum (sequenced in 2012 by the Broad Institute) exists, transcripts sequenced from both species show significant divergence. The sequence is not highly annotated, and the majority of transcripts are predicted rather than experimentally validated. Using deep RNA sequencing of the OK cell line, we characterized its transcriptome via de novo transcriptome assembly and alignment to the genome. The quality of the de novo assembled transcriptome was assessed by the extent of homology to sequences in nucleotide and protein databases. Gene expression levels in the OK cell line, from both the de novo transcriptome and genes aligned to the genome, were compared with publicly available rat kidney nephron segment expression data. Our studies demonstrate the expression in OK cells of numerous PT-specific ion transporters and other key proteins relevant for rodent and human PT function. Additionally, the sequence and expression data reported here provide an important resource for genetic manipulation and other studies on PT cell function using these cells.
[Mh] Termos MeSH primário: Células Epiteliais/metabolismo
Túbulos Renais Proximais/metabolismo
Gambás/genética
Transcriptoma
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Biologia Computacional
Bases de Dados Genéticas
Feminino
Perfilação da Expressão Gênica/métodos
Regulação da Expressão Gênica
Genótipo
Sequenciamento de Nucleotídeos em Larga Escala
Seres Humanos
Transporte de Íons
Túbulos Renais Proximais/citologia
Proteínas de Membrana Transportadoras/genética
Proteínas de Membrana Transportadoras/metabolismo
Fenótipo
Ratos
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Membrane Transport Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171005
[Lr] Data última revisão:
171005
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170616
[St] Status:MEDLINE
[do] DOI:10.1152/ajprenal.00228.2017


  6 / 4043 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28358635
[Au] Autor:Johnson EM; Nagamori Y; Duncan-Decocq RA; Whitley PN; Ramachandran A; Reichard MV
[Ti] Título:Prevalence of Alaria infection in companion animals in north central Oklahoma from 2006 through 2015 and detection in wildlife.
[So] Source:J Am Vet Med Assoc;250(8):881-886, 2017 Apr 15.
[Is] ISSN:1943-569X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE To determine the prevalence of Alaria infection in cats and dogs in north central Oklahoma over various periods and investigate whether wild animal species in this region were also infected. DESIGN Combined cross-sectional study and case series. SAMPLE Results of parasitological testing of fecal samples from 5,417 client-owned dogs and 1,246 client-owned cats (2006 through 2014); fecal samples from 837 shelter or rescue dogs and 331 shelter or rescue cats (2013 and 2014) and 268 feral cats (2015); tongue or jowl samples from cadavers of 43 wild pigs, 3 opossums, and 1 raccoon; and intestinal tract segments from cadavers of 48 cats and 5 coyotes. PROCEDURES Various parasite recovery techniques were performed to detect various Alaria stages in samples. Recovered adult trematodes and mesocercariae were used for PCR assay and sequencing of the 28S rRNA gene. RESULTS Prevalence of Alaria infection was significantly higher in feral cats (9.0%) than in shelter or rescue cats (0.6%) and client-owned cats (1.4%) and in shelter or rescue dogs (1.8%) than in client-owned dogs (0.2%). Mesocercariae were recovered from tissue samples from 11 (26%) wild pigs and 1 opossum. Amplicon sequences from adult trematodes and mesocercariae were 100% identical to each other and 99% homologous to GenBank sequences of Alaria alata and Alaria mustelae. CONCLUSIONS AND CLINICAL RELEVANCE Prevalence of Alaria infection in the study area has increased in dogs and cats since 1990, when infections were rare. Prevalence in wild pigs was similar to that in Eurasia, where A alata is considered an emerging zoonotic parasite.
[Mh] Termos MeSH primário: Animais Selvagens/parasitologia
Doenças do Gato/epidemiologia
Doenças do Cão/epidemiologia
Trematódeos/isolamento & purificação
Infecções por Trematódeos/veterinária
[Mh] Termos MeSH secundário: Animais
Doenças do Gato/etiologia
Gatos
Coiotes/parasitologia
Reservatórios de Doenças/veterinária
Doenças do Cão/etiologia
Cães
Fezes/parasitologia
Oklahoma/epidemiologia
Gambás/parasitologia
Prevalência
Estudos Prospectivos
Guaxinins/parasitologia
Estudos Retrospectivos
Suínos
Infecções por Trematódeos/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170828
[Lr] Data última revisão:
170828
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170331
[St] Status:MEDLINE
[do] DOI:10.2460/javma.250.8.881


  7 / 4043 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28356267
[Au] Autor:Eshbach ML; Kaur A; Rbaibi Y; Tejero J; Weisz OA
[Ad] Endereço:Renal-Electrolyte Division, Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania; and.
[Ti] Título:Hemoglobin inhibits albumin uptake by proximal tubule cells: implications for sickle cell disease.
[So] Source:Am J Physiol Cell Physiol;312(6):C733-C740, 2017 Jun 01.
[Is] ISSN:1522-1563
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Proximal tubule (PT) dysfunction, including tubular proteinuria, is a significant complication in young sickle cell disease (SCD) that can eventually lead to chronic kidney disease. Hemoglobin (Hb) dimers released from red blood cells upon hemolysis are filtered into the kidney and internalized by megalin/cubilin receptors into PT cells. The PT is especially sensitive to heme toxicity, and tubular dysfunction in SCD is thought to result from prolonged exposure to filtered Hb. Here we show that concentrations of Hb predicted to enter the tubule lumen during hemolytic crisis competitively inhibit the uptake of another megalin/cubilin ligand (albumin) by PT cells. These effects were independent of heme reduction state. The Glu7Val mutant of Hb that causes SCD was equally effective at inhibiting albumin uptake compared with wild-type Hb. Addition of the Hb scavenger haptoglobin (Hpt) restored albumin uptake in the presence of Hb, suggesting that Hpt binding to the Hb αß dimer-dimer interface interferes with Hb binding to megalin/cubilin. BLAST searches and structural modeling analyses revealed regions of similarity between Hb and albumin that map to this region and may represent sites of Hb interaction with megalin/cubilin. Our studies suggest that impaired endocytosis of megalin/cubilin ligands, rather than heme toxicity, may be the cause of tubular proteinuria in SCD patients. Additionally, loss of these filtered proteins into the urine may contribute to the extra-renal pathogenesis of SCD.
[Mh] Termos MeSH primário: Anemia Falciforme/metabolismo
Haptoglobinas/química
Hemoglobinas/química
Proteína-2 Relacionada a Receptor de Lipoproteína de Baixa Densidade/química
Albumina Sérica/química
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Anemia Falciforme/genética
Anemia Falciforme/patologia
Animais
Sítios de Ligação
Ligação Competitiva
Linhagem Celular
Linhagem Celular Transformada
Feminino
Haptoglobinas/metabolismo
Heme/química
Hemoglobinas/metabolismo
Hemólise
Seres Humanos
Túbulos Renais Proximais/citologia
Túbulos Renais Proximais/metabolismo
Ligantes
Proteína-2 Relacionada a Receptor de Lipoproteína de Baixa Densidade/metabolismo
Masculino
Gambás
Oxirredução
Ligação Proteica
Conformação Proteica em alfa-Hélice
Alinhamento de Sequência
Homologia de Sequência de Aminoácidos
Albumina Sérica/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (HP protein, human); 0 (Haptoglobins); 0 (Hemoglobins); 0 (Ligands); 0 (Low Density Lipoprotein Receptor-Related Protein-2); 0 (Serum Albumin); 42VZT0U6YR (Heme)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170331
[St] Status:MEDLINE
[do] DOI:10.1152/ajpcell.00021.2017


  8 / 4043 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Araújo, Silvana Marques de
Texto completo
[PMID]:28212811
[Au] Autor:Abegg CP; Abreu AP; Silva JL; Araújo SM; Gomes ML; Ferreira ÉC; Toledo MJ
[Ad] Endereço:Post-Graduation Program in Health Sciences at the State University of Maringá (Universidade Estadual de Maringá-UEM), Maringá, PR, Brazil.
[Ti] Título:Polymorphisms of blood forms and in vitro metacyclogenesis of Trypanosoma cruzi I, II, and IV.
[So] Source:Exp Parasitol;176:8-15, 2017 May.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Trypanosoma cruzi is the etiologic agent of American trypanosomiasis has broad biological and genetic diversity. Remaining to be studied are polymorphisms of the blood forms and metacyclogenesis of different T. cruzi discrete typing units (DTUs). Our goal was to evaluate the relationship between T. cruzi DTUs, the morphology of blood trypomastigotes, and in vitro metacyclogenesis. T. cruzi strains that pertained to DTUs TcI, TcII, and TcIV from different Brazilian states were used. Parameters that were related to the morphology of eight strains were assessed in thin blood smears that were obtained from mice that were inoculated with blood or culture forms, depending on strain. The metacyclogenesis of 12 strains was measured using smears with Liver Infusion Tryptose culture medium and M16 culture medium (which is poor in nutrients and has a low pH) at the exponential phase of growth, both stained with Giemsa. The morphological pattern of TcII strains was consistent with broad forms of the parasite. In TcIV strains, slender forms predominated. The Y strain (TcII) was morphologically more similar to TcIV. Significant differences in polymorphisms were observed between DTUs. Metacyclogenesis parameters, although displaying large standard deviations, differed between the DTUs, with the following descending rank order: TcII > TcI > TcIV. The mean numbers of metacyclic trypomastigotes for TcII were significantly higher than the other DTUs. Although the DTUs presented overlapping characteristics, the general pattern was that different DTUs exhibited significantly different morphologies and metacyclogenesis, suggesting that the genetic diversity of T. cruzi could be related to parameters that are associated with the evolution of infection in mammalian hosts and its ability to disperse in nature.
[Mh] Termos MeSH primário: Doença de Chagas/parasitologia
Parasitemia/parasitologia
Polimorfismo Genético
Trypanosoma cruzi/genética
[Mh] Termos MeSH secundário: Animais
Brasil/epidemiologia
Doença de Chagas/sangue
Doença de Chagas/epidemiologia
Seres Humanos
Camundongos
Gambás
Parasitemia/sangue
Parasitemia/epidemiologia
Rhodnius
Trypanosoma cruzi/classificação
Trypanosoma cruzi/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170411
[Lr] Data última revisão:
170411
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170219
[St] Status:MEDLINE


  9 / 4043 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28062306
[Au] Autor:Yamashita T; Udagawa N; Thirukonda GJ; Uehara S; Yamauchi H; Suzuki N; Li F; Kobayashi Y; Takahashi N
[Ad] Endereço:Institute for Oral Science, Matsumoto Dental University, 1780 Hirooka-Gobara, Shiojiri, Nagano 399-0781, Japan. Electronic address: teru@po.mdu.ac.jp.
[Ti] Título:Platypus and opossum calcitonins exhibit strong activities, even though they belong to mammals.
[So] Source:Gen Comp Endocrinol;246:270-278, 2017 May 15.
[Is] ISSN:1095-6840
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In mammalian assay systems, calcitonin peptides of non-mammalian species exhibit stronger activity than those of mammals. Recently, comparative analyses of a wide-range of species revealed that platypus and opossum, which diverged early from other mammals, possess calcitonins that are more similar in amino acid sequence to those of non-mammals than mammals. We herein determined whether platypus and opossum calcitonins exhibit similar biological activities to those of non-mammalian calcitonins using an assay of actin ring formation in mouse osteoclasts. We also compared the dose-dependent effects of each calcitonin on cAMP production in osteoclasts. Consistent with the strong similarities in their primary amino acid sequences, platypus and opossum calcitonins disrupted actin rings with similar efficacies to that of salmon calcitonin. Human calcitonin exhibited the weakest inhibitory potency and required a 100-fold higher concentration (EC =3×10 M) than that of salmon calcitonin (EC =2×10 M). Platypus and opossum calcitonins also induced cAMP production in osteoclast cultures with the same efficacies as that of salmon calcitonin. Thus, platypus and opossum calcitonins exhibited strong biological activities, similar to those of the salmon. In addition, phylogenetic analysis revealed that platypus and opossum calcitonins clustered with the salmon-type group but not human- or porcine-type group. These results suggest that platypus and opossum calcitonins are classified into the salmon-type group, in terms of the biological activities and amino acid sequences.
[Mh] Termos MeSH primário: Actinas/metabolismo
Conservadores da Densidade Óssea/farmacologia
Calcitonina/farmacologia
AMP Cíclico/metabolismo
Gambás/metabolismo
Osteoclastos/metabolismo
Ornitorrinco/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Animais Recém-Nascidos
Células Cultivadas
Dicroísmo Circular
Seres Humanos
Camundongos
Osteoclastos/citologia
Osteoclastos/efeitos dos fármacos
Fragmentos de Peptídeos
Filogenia
Salmão
Homologia de Sequência de Aminoácidos
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Actins); 0 (Bone Density Conservation Agents); 0 (Peptide Fragments); 9007-12-9 (Calcitonin); E0399OZS9N (Cyclic AMP)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170515
[Lr] Data última revisão:
170515
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170108
[St] Status:MEDLINE


  10 / 4043 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27288013
[Au] Autor:Coady MJ; El Tarazi A; Santer R; Bissonnette P; Sasseville LJ; Calado J; Lussier Y; Dumayne C; Bichet DG; Lapointe JY
[Ad] Endereço:Physics Department & Groupe d'étude des protéines membranaires.
[Ti] Título:MAP17 Is a Necessary Activator of Renal Na+/Glucose Cotransporter SGLT2.
[So] Source:J Am Soc Nephrol;28(1):85-93, 2017 Jan.
[Is] ISSN:1533-3450
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The renal proximal tubule reabsorbs 90% of the filtered glucose load through the Na -coupled glucose transporter SGLT2, and specific inhibitors of SGLT2 are now available to patients with diabetes to increase urinary glucose excretion. Using expression cloning, we identified an accessory protein, 17 kDa membrane-associated protein (MAP17), that increased SGLT2 activity in RNA-injected Xenopus oocytes by two orders of magnitude. Significant stimulation of SGLT2 activity also occurred in opossum kidney cells cotransfected with SGLT2 and MAP17. Notably, transfection with MAP17 did not change the quantity of SGLT2 protein at the cell surface in either cell type. To confirm the physiologic relevance of the MAP17-SGLT2 interaction, we studied a cohort of 60 individuals with familial renal glucosuria. One patient without any identifiable mutation in the SGLT2 coding gene (SLC5A2) displayed homozygosity for a splicing mutation (c.176+1G>A) in the MAP17 coding gene (PDZK1IP1). In the proximal tubule and in other tissues, MAP17 is known to interact with PDZK1, a scaffolding protein linked to other transporters, including Na /H exchanger 3, and to signaling pathways, such as the A-kinase anchor protein 2/protein kinase A pathway. Thus, these results provide the basis for a more thorough characterization of SGLT2 which would include the possible effects of its inhibition on colocalized renal transporters.
[Mh] Termos MeSH primário: Rim/metabolismo
Proteínas de Membrana/fisiologia
Transportador 2 de Glucose-Sódio/fisiologia
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Glicosúria Renal/genética
Seres Humanos
Rim/citologia
Túbulos Renais Proximais
Proteínas de Membrana/genética
Mutação
Gambás
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Membrane Proteins); 0 (PDZK1IP1 protein, human); 0 (Sodium-Glucose Transporter 2)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170602
[Lr] Data última revisão:
170602
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160612
[St] Status:MEDLINE
[do] DOI:10.1681/ASN.2015111282



página 1 de 405 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde